GenScript offers hybridoma cell culture services for monoclonal antibody production at flexible scales, up to gram level quantities. We will carry out the hybridoma cell culture and purification, and deliver protein A/G-purified antibodies to you.

Our default production service utilizes the low bovine IgG (2%) roller bottle method and does not include an endotoxin level guarantee. However, serum-free roller bottle (no bovine IgG) and low EU (≤ 3 EU/mg) production options are also available. Additionally, if you generated your rodent IgG hybridoma using GenScript’s antibody services, we are pleased to guarantee an antibody yield greater than 15 mg/L.

Service Highlights

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Competitive pricing
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Flexible production scale ranging from milligram to gram level
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Guaranteed antibody production yield ≥ 15 mg/L (GenScript-generated rodent hybridomas only)
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High purity (≥ 90%) with low bovine IgG (2%) for normal roller bottle service
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Serum free & low endotoxin (≤ 3 EU/mg) antibody production service options

Service Details

  Description Time Line
Phase I: Cell line submission, recovery, adoption and expansion

Customer submits:

  • 2 vials of frozen hybridoma cells
  • Culture medium recipe of submitted hybridoma cells
  • Name of species from which hybridoma cell line was derived
  • Production history of submitted hybridoma cell line (if available)

After the materials and information listed above have been received, the hybridoma cell line will be recovered and examined for contamination. If no contamination is detected, the cell line will be adopted to production medium and expanded.

1-2 weeks
Phase II: Inoculation

The expanded cell culture will be transferred to a roller bottle containing hybridoma cell culture medium and placed on a roller device in a 37°C incubator.

Cell growth status will be monitored daily over the course of the entire hybridoma cell culture process.

2 weeks
Phase III: Harvest and antibody purification

Cells are harvested from culture medium by centrifugation.

The antibody-containing culture supernatant is processed for antibody purification by protein A/G.

After QC, the purified antibodies are ready for delivery.

1 week

Service Packages

Our hybridoma roller bottle cell culture antibody production service is scalable. Additional options such as serum-free and low endotoxin production are also available. Please do not hesitate to inquire by e-mailing [email protected].

Cat. No. Service Package Name
Hybridoma cell culture antibody production service, 10 – 15 mg
Low endotoxin hybridoma cell culture antibody production service, 10 – 15 mg

For upstream hybridoma development, please visit our MonoExpress™ Monoclonal Antibody Services – the fastest monoclonal antibody production services that offer all application guarantees. See details »

For downstream recombinant antibody expression, GenScript has launched its proprietary TurboCHO™ platform, which offers higher yield performance within a shorter time period. This cost-effective production system enables small to large-scale (microgram to kilogram) antibody production with a high level of consistency. See Details »

Get in Touch
with GenScript Custom
Antibody Services

Get in Touch with GenScript Custom Antibody Services
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Quotation and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

Fill out the online form and email to [email protected]
A Technical Account Manager will reply you within 24 hours to finalize the quote

To place order, provide credit card/PO information

Production of your project begins immediately
A Project Manager is in contact with you during the production process to give updates



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