RUSC2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	RUSC2
Entrez Gene ID	106894067
Full Name	RUN and SH3 domain containing 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014952514.1	XP_014808000.1	iporin isoform X1
XM_014952515.1	XP_014808001.1	iporin isoform X1
XM_014952516.1	XP_014808002.1	iporin isoform X1
XM_014952518.1	XP_014808004.1	iporin isoform X1
XM_014952520.1	XP_014808006.1	iporin isoform X2
XM_014952521.1	XP_014808007.1	iporin isoform X3
XM_014952519.1	XP_014808005.1	iporin isoform X1
XM_014952517.1	XP_014808003.1	iporin isoform X1

Pathways from BioSystems
Gene Ontology

The following RUSC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RUSC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa72709	XM_014952515.1 Latest version!	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa72709	XM_014952514.1 Latest version!	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa72709	XM_014952518.1 Latest version!	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa72709	XM_014952517.1 Latest version!	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa72709	XM_014952516.1 Latest version!	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa72709	XM_014952519.1 Latest version!	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa72710	XM_014952520.1 Latest version!	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa72711	XM_014952521.1 Latest version!	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RUSC2 ( XM_014952514.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808000.1 Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa72709
Clone ID Related Accession (Same CDS sequence)	XM_014952514.1 , XM_014952515.1 , XM_014952516.1 , XM_014952518.1 , XM_014952519.1 , XM_014952517.1
Accession Version	XM_014952514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090963.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808000.1
CDS	387..5231
Translation	MDSPPKLTGETLIVHHIPLVHCQVPDRQCCSVSKRTNPFCQPELSITRNSALPDRDLSQTDSLVYSSFLQTSETSAEASDNKEGKAKDLIVPSVSKRHNPFLLSEGEDLSIFGDDLGQKSFHLHNSLVAGKPPFQLHELALPPFHLHDSNHIVKSWNMASRSGVVDGQEDKISSDDIQKRNNANRCHQASERMELDECSCHRGSSSAFSFDGGDQEWNQNTGELLRNQDALRSRTCSCSSSELQHCRCYSSSSQSEVIDQQMGYISDSSCNSSDGVLVNFSALYNKMNGHSRSNLNSANLSCDSSFCSHSDTGAFYLDLHSSPTESKMSCESHHPENSEKVCGCQHSSSPVLDANCNSYHLHCEPCASESSDLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEDHTKGSPAQPTEYYLFRRPDLRQEEDNVECSEEEAKGEATQNMIEGQVYVNVSPPNLNTSRQRSRSYDQNLDRSPSSRLGSLERMVSCPVKLSESPAIPIQSSPPKRVTSFAELAKGRKKNGTSPPLRSSGDSSLEFSPIPETQRDCPTFLEERARRSQSLPPMPFVHGLNRSCDGFCLNHTFGDSQAACSTKDSVSSEKVPSGHGAGEQASLSLLMEADASFSGGSASGHGQRDVRARADGGGTDGKPVVRYSKDQRPTTLPIQPFVFQHHFSKPPKARALHSHFASSLSQLYSLSSNRPASQQISSNSQSSAPATAAEQPAAVGSQAHSTLLTQRSVDGAASRNDGCVKKAAPETTRPSPLGSYSPVRCNVPFFQSVDSSSSPTAERAGESQPPRSRSCPISASLLPTRSSPAVSAVQPSQATKADALKQKENPKPVPKKEAPLEAGPPLSEYRLHSVSLPPLSVGGMPMSRVGGHADPHWRSGSETSSSGPLNSMGTRPLNANHLSPQALKWREYRRRNPLGLDRVSGLPGLASSLDRRQQEPRLNRGNTIFELPGSLNTSHFHCKLNGQSMRQLQLTYTDFFPDYFSLAEKPPAEFCLSPDGNTESISIDLLQKKGLVKAINTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLILKYLCPAVRDILSDGLKAYVLDMIIGQRRNIPWSVVEASTQLGPSTKLLHSLYTKISQYTELTNHTMRFNAFLFGLLNIRSLEFWFNHLYNHEDIILAHYQPVGFLCLSHSVCQPLFEELLLLLQPLSLLPFNLDLLFEHHLMQMGKEQQQQKELLRVKQDLLLSAHSTLQLMRTRGSSDDPDGCSTAPEAGKVGTTDGDISPGHSSQQAASERVKGVGASCGDVEKDEERRKVRESTWEGKKDKQAGWWYQLMQSSQIYIEGSPEGSKFIRYEKKKKALNDVPRSAETHKAPPPREGVVEGAEACPIAEGTLEERPKAASKPSPEAVEEPLEKPQQVAVSEEVKERSWPFWMGSPPDSVVTELKRSKEKEAGTQQKVGAAAAAPQEESNTSASEGSQTIKWGHLFGSRKAQKEPRQPNRLPSGWLSLDKSVFQLVAQTVGASMWREAAPEPERPQPASPEVPPIGHPARGLSPHPPCEVKALCHHIATEAGQLSFNKGDILQVISKVDGDWLQCSLGSEKGLVPIMYVTHPEDEDY

Target ORF information:

RefSeq Version	XM_014952514.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952514.1

ORF Insert Sequence:

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUSC2 ( XM_014952515.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808001.1 Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa72709
Clone ID Related Accession (Same CDS sequence)	XM_014952514.1 , XM_014952515.1 , XM_014952516.1 , XM_014952518.1 , XM_014952519.1 , XM_014952517.1
Accession Version	XM_014952515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090963.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808001.1
CDS	229..5073
Translation	MDSPPKLTGETLIVHHIPLVHCQVPDRQCCSVSKRTNPFCQPELSITRNSALPDRDLSQTDSLVYSSFLQTSETSAEASDNKEGKAKDLIVPSVSKRHNPFLLSEGEDLSIFGDDLGQKSFHLHNSLVAGKPPFQLHELALPPFHLHDSNHIVKSWNMASRSGVVDGQEDKISSDDIQKRNNANRCHQASERMELDECSCHRGSSSAFSFDGGDQEWNQNTGELLRNQDALRSRTCSCSSSELQHCRCYSSSSQSEVIDQQMGYISDSSCNSSDGVLVNFSALYNKMNGHSRSNLNSANLSCDSSFCSHSDTGAFYLDLHSSPTESKMSCESHHPENSEKVCGCQHSSSPVLDANCNSYHLHCEPCASESSDLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEDHTKGSPAQPTEYYLFRRPDLRQEEDNVECSEEEAKGEATQNMIEGQVYVNVSPPNLNTSRQRSRSYDQNLDRSPSSRLGSLERMVSCPVKLSESPAIPIQSSPPKRVTSFAELAKGRKKNGTSPPLRSSGDSSLEFSPIPETQRDCPTFLEERARRSQSLPPMPFVHGLNRSCDGFCLNHTFGDSQAACSTKDSVSSEKVPSGHGAGEQASLSLLMEADASFSGGSASGHGQRDVRARADGGGTDGKPVVRYSKDQRPTTLPIQPFVFQHHFSKPPKARALHSHFASSLSQLYSLSSNRPASQQISSNSQSSAPATAAEQPAAVGSQAHSTLLTQRSVDGAASRNDGCVKKAAPETTRPSPLGSYSPVRCNVPFFQSVDSSSSPTAERAGESQPPRSRSCPISASLLPTRSSPAVSAVQPSQATKADALKQKENPKPVPKKEAPLEAGPPLSEYRLHSVSLPPLSVGGMPMSRVGGHADPHWRSGSETSSSGPLNSMGTRPLNANHLSPQALKWREYRRRNPLGLDRVSGLPGLASSLDRRQQEPRLNRGNTIFELPGSLNTSHFHCKLNGQSMRQLQLTYTDFFPDYFSLAEKPPAEFCLSPDGNTESISIDLLQKKGLVKAINTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLILKYLCPAVRDILSDGLKAYVLDMIIGQRRNIPWSVVEASTQLGPSTKLLHSLYTKISQYTELTNHTMRFNAFLFGLLNIRSLEFWFNHLYNHEDIILAHYQPVGFLCLSHSVCQPLFEELLLLLQPLSLLPFNLDLLFEHHLMQMGKEQQQQKELLRVKQDLLLSAHSTLQLMRTRGSSDDPDGCSTAPEAGKVGTTDGDISPGHSSQQAASERVKGVGASCGDVEKDEERRKVRESTWEGKKDKQAGWWYQLMQSSQIYIEGSPEGSKFIRYEKKKKALNDVPRSAETHKAPPPREGVVEGAEACPIAEGTLEERPKAASKPSPEAVEEPLEKPQQVAVSEEVKERSWPFWMGSPPDSVVTELKRSKEKEAGTQQKVGAAAAAPQEESNTSASEGSQTIKWGHLFGSRKAQKEPRQPNRLPSGWLSLDKSVFQLVAQTVGASMWREAAPEPERPQPASPEVPPIGHPARGLSPHPPCEVKALCHHIATEAGQLSFNKGDILQVISKVDGDWLQCSLGSEKGLVPIMYVTHPEDEDY

Target ORF information:

RefSeq Version	XM_014952515.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952515.1

ORF Insert Sequence:

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUSC2 ( XM_014952516.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808002.1 Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X3, mRNA.

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CloneID	OCa72709
Clone ID Related Accession (Same CDS sequence)	XM_014952514.1 , XM_014952515.1 , XM_014952516.1 , XM_014952518.1 , XM_014952519.1 , XM_014952517.1
Accession Version	XM_014952516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090963.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808002.1
CDS	223..5067
Translation	MDSPPKLTGETLIVHHIPLVHCQVPDRQCCSVSKRTNPFCQPELSITRNSALPDRDLSQTDSLVYSSFLQTSETSAEASDNKEGKAKDLIVPSVSKRHNPFLLSEGEDLSIFGDDLGQKSFHLHNSLVAGKPPFQLHELALPPFHLHDSNHIVKSWNMASRSGVVDGQEDKISSDDIQKRNNANRCHQASERMELDECSCHRGSSSAFSFDGGDQEWNQNTGELLRNQDALRSRTCSCSSSELQHCRCYSSSSQSEVIDQQMGYISDSSCNSSDGVLVNFSALYNKMNGHSRSNLNSANLSCDSSFCSHSDTGAFYLDLHSSPTESKMSCESHHPENSEKVCGCQHSSSPVLDANCNSYHLHCEPCASESSDLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEDHTKGSPAQPTEYYLFRRPDLRQEEDNVECSEEEAKGEATQNMIEGQVYVNVSPPNLNTSRQRSRSYDQNLDRSPSSRLGSLERMVSCPVKLSESPAIPIQSSPPKRVTSFAELAKGRKKNGTSPPLRSSGDSSLEFSPIPETQRDCPTFLEERARRSQSLPPMPFVHGLNRSCDGFCLNHTFGDSQAACSTKDSVSSEKVPSGHGAGEQASLSLLMEADASFSGGSASGHGQRDVRARADGGGTDGKPVVRYSKDQRPTTLPIQPFVFQHHFSKPPKARALHSHFASSLSQLYSLSSNRPASQQISSNSQSSAPATAAEQPAAVGSQAHSTLLTQRSVDGAASRNDGCVKKAAPETTRPSPLGSYSPVRCNVPFFQSVDSSSSPTAERAGESQPPRSRSCPISASLLPTRSSPAVSAVQPSQATKADALKQKENPKPVPKKEAPLEAGPPLSEYRLHSVSLPPLSVGGMPMSRVGGHADPHWRSGSETSSSGPLNSMGTRPLNANHLSPQALKWREYRRRNPLGLDRVSGLPGLASSLDRRQQEPRLNRGNTIFELPGSLNTSHFHCKLNGQSMRQLQLTYTDFFPDYFSLAEKPPAEFCLSPDGNTESISIDLLQKKGLVKAINTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLILKYLCPAVRDILSDGLKAYVLDMIIGQRRNIPWSVVEASTQLGPSTKLLHSLYTKISQYTELTNHTMRFNAFLFGLLNIRSLEFWFNHLYNHEDIILAHYQPVGFLCLSHSVCQPLFEELLLLLQPLSLLPFNLDLLFEHHLMQMGKEQQQQKELLRVKQDLLLSAHSTLQLMRTRGSSDDPDGCSTAPEAGKVGTTDGDISPGHSSQQAASERVKGVGASCGDVEKDEERRKVRESTWEGKKDKQAGWWYQLMQSSQIYIEGSPEGSKFIRYEKKKKALNDVPRSAETHKAPPPREGVVEGAEACPIAEGTLEERPKAASKPSPEAVEEPLEKPQQVAVSEEVKERSWPFWMGSPPDSVVTELKRSKEKEAGTQQKVGAAAAAPQEESNTSASEGSQTIKWGHLFGSRKAQKEPRQPNRLPSGWLSLDKSVFQLVAQTVGASMWREAAPEPERPQPASPEVPPIGHPARGLSPHPPCEVKALCHHIATEAGQLSFNKGDILQVISKVDGDWLQCSLGSEKGLVPIMYVTHPEDEDY

Target ORF information:

RefSeq Version	XM_014952516.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952516.1

ORF Insert Sequence:

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUSC2 ( XM_014952518.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808004.1 Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X5, mRNA.

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CloneID	OCa72709
Clone ID Related Accession (Same CDS sequence)	XM_014952514.1 , XM_014952515.1 , XM_014952516.1 , XM_014952518.1 , XM_014952519.1 , XM_014952517.1
Accession Version	XM_014952518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090963.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808004.1
CDS	223..5067
Translation	MDSPPKLTGETLIVHHIPLVHCQVPDRQCCSVSKRTNPFCQPELSITRNSALPDRDLSQTDSLVYSSFLQTSETSAEASDNKEGKAKDLIVPSVSKRHNPFLLSEGEDLSIFGDDLGQKSFHLHNSLVAGKPPFQLHELALPPFHLHDSNHIVKSWNMASRSGVVDGQEDKISSDDIQKRNNANRCHQASERMELDECSCHRGSSSAFSFDGGDQEWNQNTGELLRNQDALRSRTCSCSSSELQHCRCYSSSSQSEVIDQQMGYISDSSCNSSDGVLVNFSALYNKMNGHSRSNLNSANLSCDSSFCSHSDTGAFYLDLHSSPTESKMSCESHHPENSEKVCGCQHSSSPVLDANCNSYHLHCEPCASESSDLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEDHTKGSPAQPTEYYLFRRPDLRQEEDNVECSEEEAKGEATQNMIEGQVYVNVSPPNLNTSRQRSRSYDQNLDRSPSSRLGSLERMVSCPVKLSESPAIPIQSSPPKRVTSFAELAKGRKKNGTSPPLRSSGDSSLEFSPIPETQRDCPTFLEERARRSQSLPPMPFVHGLNRSCDGFCLNHTFGDSQAACSTKDSVSSEKVPSGHGAGEQASLSLLMEADASFSGGSASGHGQRDVRARADGGGTDGKPVVRYSKDQRPTTLPIQPFVFQHHFSKPPKARALHSHFASSLSQLYSLSSNRPASQQISSNSQSSAPATAAEQPAAVGSQAHSTLLTQRSVDGAASRNDGCVKKAAPETTRPSPLGSYSPVRCNVPFFQSVDSSSSPTAERAGESQPPRSRSCPISASLLPTRSSPAVSAVQPSQATKADALKQKENPKPVPKKEAPLEAGPPLSEYRLHSVSLPPLSVGGMPMSRVGGHADPHWRSGSETSSSGPLNSMGTRPLNANHLSPQALKWREYRRRNPLGLDRVSGLPGLASSLDRRQQEPRLNRGNTIFELPGSLNTSHFHCKLNGQSMRQLQLTYTDFFPDYFSLAEKPPAEFCLSPDGNTESISIDLLQKKGLVKAINTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLILKYLCPAVRDILSDGLKAYVLDMIIGQRRNIPWSVVEASTQLGPSTKLLHSLYTKISQYTELTNHTMRFNAFLFGLLNIRSLEFWFNHLYNHEDIILAHYQPVGFLCLSHSVCQPLFEELLLLLQPLSLLPFNLDLLFEHHLMQMGKEQQQQKELLRVKQDLLLSAHSTLQLMRTRGSSDDPDGCSTAPEAGKVGTTDGDISPGHSSQQAASERVKGVGASCGDVEKDEERRKVRESTWEGKKDKQAGWWYQLMQSSQIYIEGSPEGSKFIRYEKKKKALNDVPRSAETHKAPPPREGVVEGAEACPIAEGTLEERPKAASKPSPEAVEEPLEKPQQVAVSEEVKERSWPFWMGSPPDSVVTELKRSKEKEAGTQQKVGAAAAAPQEESNTSASEGSQTIKWGHLFGSRKAQKEPRQPNRLPSGWLSLDKSVFQLVAQTVGASMWREAAPEPERPQPASPEVPPIGHPARGLSPHPPCEVKALCHHIATEAGQLSFNKGDILQVISKVDGDWLQCSLGSEKGLVPIMYVTHPEDEDY

Target ORF information:

RefSeq Version	XM_014952518.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952518.1

ORF Insert Sequence:

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUSC2 ( XM_014952519.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808005.1 Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X6, mRNA.

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CloneID	OCa72709
Clone ID Related Accession (Same CDS sequence)	XM_014952514.1 , XM_014952515.1 , XM_014952516.1 , XM_014952518.1 , XM_014952519.1 , XM_014952517.1
Accession Version	XM_014952519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090963.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808005.1
CDS	710..5554
Translation	MDSPPKLTGETLIVHHIPLVHCQVPDRQCCSVSKRTNPFCQPELSITRNSALPDRDLSQTDSLVYSSFLQTSETSAEASDNKEGKAKDLIVPSVSKRHNPFLLSEGEDLSIFGDDLGQKSFHLHNSLVAGKPPFQLHELALPPFHLHDSNHIVKSWNMASRSGVVDGQEDKISSDDIQKRNNANRCHQASERMELDECSCHRGSSSAFSFDGGDQEWNQNTGELLRNQDALRSRTCSCSSSELQHCRCYSSSSQSEVIDQQMGYISDSSCNSSDGVLVNFSALYNKMNGHSRSNLNSANLSCDSSFCSHSDTGAFYLDLHSSPTESKMSCESHHPENSEKVCGCQHSSSPVLDANCNSYHLHCEPCASESSDLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEDHTKGSPAQPTEYYLFRRPDLRQEEDNVECSEEEAKGEATQNMIEGQVYVNVSPPNLNTSRQRSRSYDQNLDRSPSSRLGSLERMVSCPVKLSESPAIPIQSSPPKRVTSFAELAKGRKKNGTSPPLRSSGDSSLEFSPIPETQRDCPTFLEERARRSQSLPPMPFVHGLNRSCDGFCLNHTFGDSQAACSTKDSVSSEKVPSGHGAGEQASLSLLMEADASFSGGSASGHGQRDVRARADGGGTDGKPVVRYSKDQRPTTLPIQPFVFQHHFSKPPKARALHSHFASSLSQLYSLSSNRPASQQISSNSQSSAPATAAEQPAAVGSQAHSTLLTQRSVDGAASRNDGCVKKAAPETTRPSPLGSYSPVRCNVPFFQSVDSSSSPTAERAGESQPPRSRSCPISASLLPTRSSPAVSAVQPSQATKADALKQKENPKPVPKKEAPLEAGPPLSEYRLHSVSLPPLSVGGMPMSRVGGHADPHWRSGSETSSSGPLNSMGTRPLNANHLSPQALKWREYRRRNPLGLDRVSGLPGLASSLDRRQQEPRLNRGNTIFELPGSLNTSHFHCKLNGQSMRQLQLTYTDFFPDYFSLAEKPPAEFCLSPDGNTESISIDLLQKKGLVKAINTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLILKYLCPAVRDILSDGLKAYVLDMIIGQRRNIPWSVVEASTQLGPSTKLLHSLYTKISQYTELTNHTMRFNAFLFGLLNIRSLEFWFNHLYNHEDIILAHYQPVGFLCLSHSVCQPLFEELLLLLQPLSLLPFNLDLLFEHHLMQMGKEQQQQKELLRVKQDLLLSAHSTLQLMRTRGSSDDPDGCSTAPEAGKVGTTDGDISPGHSSQQAASERVKGVGASCGDVEKDEERRKVRESTWEGKKDKQAGWWYQLMQSSQIYIEGSPEGSKFIRYEKKKKALNDVPRSAETHKAPPPREGVVEGAEACPIAEGTLEERPKAASKPSPEAVEEPLEKPQQVAVSEEVKERSWPFWMGSPPDSVVTELKRSKEKEAGTQQKVGAAAAAPQEESNTSASEGSQTIKWGHLFGSRKAQKEPRQPNRLPSGWLSLDKSVFQLVAQTVGASMWREAAPEPERPQPASPEVPPIGHPARGLSPHPPCEVKALCHHIATEAGQLSFNKGDILQVISKVDGDWLQCSLGSEKGLVPIMYVTHPEDEDY

Target ORF information:

RefSeq Version	XM_014952519.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952519.1

ORF Insert Sequence:

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUSC2 ( XM_014952517.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808003.1 Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X4, mRNA.

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CloneID	OCa72709
Clone ID Related Accession (Same CDS sequence)	XM_014952514.1 , XM_014952515.1 , XM_014952516.1 , XM_014952518.1 , XM_014952519.1 , XM_014952517.1
Accession Version	XM_014952517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090963.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808003.1
CDS	336..5180
Translation	MDSPPKLTGETLIVHHIPLVHCQVPDRQCCSVSKRTNPFCQPELSITRNSALPDRDLSQTDSLVYSSFLQTSETSAEASDNKEGKAKDLIVPSVSKRHNPFLLSEGEDLSIFGDDLGQKSFHLHNSLVAGKPPFQLHELALPPFHLHDSNHIVKSWNMASRSGVVDGQEDKISSDDIQKRNNANRCHQASERMELDECSCHRGSSSAFSFDGGDQEWNQNTGELLRNQDALRSRTCSCSSSELQHCRCYSSSSQSEVIDQQMGYISDSSCNSSDGVLVNFSALYNKMNGHSRSNLNSANLSCDSSFCSHSDTGAFYLDLHSSPTESKMSCESHHPENSEKVCGCQHSSSPVLDANCNSYHLHCEPCASESSDLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEDHTKGSPAQPTEYYLFRRPDLRQEEDNVECSEEEAKGEATQNMIEGQVYVNVSPPNLNTSRQRSRSYDQNLDRSPSSRLGSLERMVSCPVKLSESPAIPIQSSPPKRVTSFAELAKGRKKNGTSPPLRSSGDSSLEFSPIPETQRDCPTFLEERARRSQSLPPMPFVHGLNRSCDGFCLNHTFGDSQAACSTKDSVSSEKVPSGHGAGEQASLSLLMEADASFSGGSASGHGQRDVRARADGGGTDGKPVVRYSKDQRPTTLPIQPFVFQHHFSKPPKARALHSHFASSLSQLYSLSSNRPASQQISSNSQSSAPATAAEQPAAVGSQAHSTLLTQRSVDGAASRNDGCVKKAAPETTRPSPLGSYSPVRCNVPFFQSVDSSSSPTAERAGESQPPRSRSCPISASLLPTRSSPAVSAVQPSQATKADALKQKENPKPVPKKEAPLEAGPPLSEYRLHSVSLPPLSVGGMPMSRVGGHADPHWRSGSETSSSGPLNSMGTRPLNANHLSPQALKWREYRRRNPLGLDRVSGLPGLASSLDRRQQEPRLNRGNTIFELPGSLNTSHFHCKLNGQSMRQLQLTYTDFFPDYFSLAEKPPAEFCLSPDGNTESISIDLLQKKGLVKAINTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLILKYLCPAVRDILSDGLKAYVLDMIIGQRRNIPWSVVEASTQLGPSTKLLHSLYTKISQYTELTNHTMRFNAFLFGLLNIRSLEFWFNHLYNHEDIILAHYQPVGFLCLSHSVCQPLFEELLLLLQPLSLLPFNLDLLFEHHLMQMGKEQQQQKELLRVKQDLLLSAHSTLQLMRTRGSSDDPDGCSTAPEAGKVGTTDGDISPGHSSQQAASERVKGVGASCGDVEKDEERRKVRESTWEGKKDKQAGWWYQLMQSSQIYIEGSPEGSKFIRYEKKKKALNDVPRSAETHKAPPPREGVVEGAEACPIAEGTLEERPKAASKPSPEAVEEPLEKPQQVAVSEEVKERSWPFWMGSPPDSVVTELKRSKEKEAGTQQKVGAAAAAPQEESNTSASEGSQTIKWGHLFGSRKAQKEPRQPNRLPSGWLSLDKSVFQLVAQTVGASMWREAAPEPERPQPASPEVPPIGHPARGLSPHPPCEVKALCHHIATEAGQLSFNKGDILQVISKVDGDWLQCSLGSEKGLVPIMYVTHPEDEDY

Target ORF information:

RefSeq Version	XM_014952517.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952517.1

ORF Insert Sequence:

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GCGAACCACC TCTCCCCCCA AGCCCTGAAG TGGAGGGAGT ACAGGCGGAG GAACCCCCTG 
GGTCTGGACC GTGTTTCGGG GCTGCCTGGC TTAGCAAGCA GCCTAGACAG GAGGCAGCAA 
GAGCCCCGGC TGAACCGGGG GAACACCATC TTTGAGCTCC CCGGCAGCCT CAACACCAGC 
CATTTCCACT GCAAGCTGAA CGGACAGTCA ATGAGGCAAC TCCAGCTTAC CTACACCGAC 
TTCTTCCCCG ACTACTTCTC ACTAGCAGAG AAACCCCCAG CTGAGTTCTG CCTCTCCCCA 
GATGGCAACA CAGAGTCTAT CTCCATCGAC TTGCTACAGA AGAAGGGTCT GGTGAAGGCC 
ATCAACACTG CTGTTGACCT GATTGTGGCT CACTTTGGAA CCAGCAGGGA TCCAGGAGTG 
AAGGCCAAAC TTGGGAACAG CTCTGTGAGC CCCAACGTGG GGCATCTCAT CCTGAAATAC 
CTATGCCCTG CTGTCCGGGA CATCCTGAGT GACGGGCTTA AGGCTTACGT CCTGGACATG 
ATCATTGGCC AAAGGAGGAA CATCCCCTGG AGCGTGGTGG AGGCATCGAC TCAGCTTGGC 
CCCTCCACCA AGCTGCTGCA CAGCCTCTAT ACCAAGATCA GCCAGTACAC AGAGCTCACC 
AACCACACCA TGAGGTTCAA CGCGTTCCTC TTTGGCCTCC TCAATATCCG GTCCTTGGAG 
TTCTGGTTCA ACCACCTCTA CAACCATGAA GATATCATCC TGGCACACTA CCAGCCGGTG 
GGCTTCTTGT GCCTGTCTCA CAGCGTGTGC CAGCCTCTTT TCGAGGAGCT CCTGCTCCTG 
CTGCAGCCGC TCTCCCTGCT GCCCTTCAAC CTGGACCTCC TTTTCGAGCA CCACCTGATG 
CAGATGGGCA AAGAGCAGCA GCAGCAAAAG GAGCTGCTGC GTGTGAAGCA GGACCTGCTG 
CTGTCCGCCC ACTCCACCCT GCAGCTGATG CGGACACGGG GCAGCAGCGA CGATCCCGAC 
GGCTGCAGCA CCGCTCCCGA AGCAGGCAAA GTGGGCACCA CAGATGGTGA CATCTCTCCA 
GGCCACAGCT CCCAGCAAGC TGCCAGCGAG AGGGTGAAGG GGGTGGGGGC CTCCTGTGGA 
GATGTGGAGA AGGATGAAGA GCGGAGGAAG GTGCGGGAGA GCACGTGGGA GGGGAAGAAG 
GACAAGCAGG CGGGCTGGTG GTACCAGCTC ATGCAGAGCT CTCAGATTTA CATCGAGGGC 
TCACCCGAAG GCTCGAAGTT CATCCGCTAT GAGAAGAAGA AGAAGGCTCT GAATGATGTT 
CCCAGGTCAG CGGAGACCCA CAAGGCACCA CCACCCCGCG AAGGAGTGGT GGAAGGGGCA 
GAGGCATGCC CCATTGCTGA GGGCACCTTG GAGGAGAGAC CCAAGGCTGC CAGCAAGCCA 
AGTCCTGAAG CTGTGGAAGA GCCCTTGGAA AAGCCCCAGC AGGTAGCAGT CAGTGAAGAG 
GTGAAGGAAC GGAGTTGGCC CTTCTGGATG GGCAGCCCCC CAGACTCGGT GGTTACAGAG 
CTGAAGCGCA GCAAGGAGAA GGAGGCTGGG ACTCAGCAAA AAGTGGGAGC AGCAGCAGCA 
GCCCCTCAAG AGGAGAGCAA CACCAGTGCA TCAGAGGGCA GCCAGACCAT CAAGTGGGGA 
CACTTGTTTG GGTCCAGGAA GGCTCAGAAA GAGCCCAGGC AGCCTAACAG GTTGCCCTCT 
GGCTGGCTGA GTTTGGACAA GTCCGTGTTC CAGCTGGTGG CCCAGACGGT TGGGGCAAGC 
ATGTGGCGAG AGGCAGCTCC TGAGCCAGAA CGCCCCCAGC CAGCGTCACC TGAAGTGCCC 
CCCATAGGGC ATCCAGCCCG GGGGCTGTCT CCTCACCCTC CCTGTGAGGT GAAGGCTCTT 
TGCCATCACA TTGCCACCGA GGCAGGACAA CTGAGCTTCA ACAAAGGGGA TATCTTGCAA 
GTCATCTCCA AAGTGGATGG TGACTGGCTC CAGTGCAGCC TCGGCTCTGA GAAGGGACTG 
GTGCCCATCA TGTACGTCAC CCACCCGGAG GACGAGGACT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUSC2 ( XM_014952520.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808006.1 Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCa72710
Clone ID Related Accession (Same CDS sequence)	XM_014952520.1
Accession Version	XM_014952520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	iporin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090963.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGGTGGCAC AGACGGCAAG CCCGTGGTAC GCTACAGCAA GGACCAGCGT 
CCCACGACTC TGCCCATCCA GCCCTTTGTT TTCCAGCACC ACTTCAGCAA GCCACCCAAG 
GCCCGTGCCC TGCACAGCCA TTTTGCCTCC AGCCTTTCCC AACTCTACAG CTTGTCCAGC 
AACCGACCTG CCAGTCAGCA GATCTCCTCC AATTCCCAGT CCTCGGCCCC GGCCACTGCG 
GCAGAGCAGC CGGCGGCGGT GGGGAGCCAG GCCCACAGCA CGCTCCTGAC CCAACGCTCG 
GTGGATGGAG CTGCCTCCCG CAACGATGGC TGCGTTAAGA AGGCTGCCCC GGAGACAACC 
CGGCCGTCCC CCCTGGGGAG TTACTCTCCT GTGCGATGCA ACGTGCCTTT CTTCCAAAGC 
GTGGACTCTT CTTCCTCGCC CACCGCCGAG AGAGCTGGAG AGAGCCAGCC CCCCAGGAGC 
AGGTCCTGCC CCATCTCCGC CAGCCTGCTT CCCACCAGGT CTTCCCCTGC GGTCAGTGCT 
GTGCAGCCCT CCCAAGCCAC CAAAGCTGAT GCCCTGAAGC AGAAGGAGAA CCCCAAGCCG 
GTTCCCAAGA AGGAGGCACC GCTGGAAGCA GGCCCACCAC TCTCTGAGTA CCGACTCCAC 
AGTGTGTCCC TCCCACCGCT CTCGGTGGGT GGCATGCCCA TGAGCAGAGT GGGAGGCCAC 
GCAGATCCCC ACTGGAGAAG TGGCAGTGAG ACCAGCAGCT CTGGTCCCCT GAACAGCATG 
GGAACACGGC CCCTCAATGC GAACCACCTC TCCCCCCAAG CCCTGAAGTG GAGGGAGTAC 
AGGCGGAGGA ACCCCCTGGG TCTGGACCGT GTTTCGGGGC TGCCTGGCTT AGCAAGCAGC 
CTAGACAGGA GGCAGCAAGA GCCCCGGCTG AACCGGGGGA ACACCATCTT TGAGCTCCCC 
GGCAGCCTCA ACACCAGCCA TTTCCACTGC AAGCTGAACG GACAGTCAAT GAGGCAACTC 
CAGCTTACCT ACACCGACTT CTTCCCCGAC TACTTCTCAC TAGCAGAGAA ACCCCCAGCT 
GAGTTCTGCC TCTCCCCAGA TGGCAACACA GAGTCTATCT CCATCGACTT GCTACAGAAG 
AAGGGTCTGG TGAAGGCCAT CAACACTGCT GTTGACCTGA TTGTGGCTCA CTTTGGAACC 
AGCAGGGATC CAGGAGTGAA GGCCAAACTT GGGAACAGCT CTGTGAGCCC CAACGTGGGG 
CATCTCATCC TGAAATACCT ATGCCCTGCT GTCCGGGACA TCCTGAGTGA CGGGCTTAAG 
GCTTACGTCC TGGACATGAT CATTGGCCAA AGGAGGAACA TCCCCTGGAG CGTGGTGGAG 
GCATCGACTC AGCTTGGCCC CTCCACCAAG CTGCTGCACA GCCTCTATAC CAAGATCAGC 
CAGTACACAG AGCTCACCAA CCACACCATG AGGTTCAACG CGTTCCTCTT TGGCCTCCTC 
AATATCCGGT CCTTGGAGTT CTGGTTCAAC CACCTCTACA ACCATGAAGA TATCATCCTG 
GCACACTACC AGCCGGTGGG CTTCTTGTGC CTGTCTCACA GCGTGTGCCA GCCTCTTTTC 
GAGGAGCTCC TGCTCCTGCT GCAGCCGCTC TCCCTGCTGC CCTTCAACCT GGACCTCCTT 
TTCGAGCACC ACCTGATGCA GATGGGCAAA GAGCAGCAGC AGCAAAAGGA GCTGCTGCGT 
GTGAAGCAGG ACCTGCTGCT GTCCGCCCAC TCCACCCTGC AGCTGATGCG GACACGGGGC 
AGCAGCGACG ATCCCGACGG CTGCAGCACC GCTCCCGAAG CAGGCAAAGT GGGCACCACA 
GATGGTGACA TCTCTCCAGG CCACAGCTCC CAGCAAGCTG CCAGCGAGAG GGTGAAGGGG 
GTGGGGGCCT CCTGTGGAGA TGTGGAGAAG GATGAAGAGC GGAGGAAGGT GCGGGAGAGC 
ACGTGGGAGG GGAAGAAGGA CAAGCAGGCG GGCTGGTGGT ACCAGCTCAT GCAGAGCTCT 
CAGATTTACA TCGAGGGCTC ACCCGAAGGC TCGAAGTTCA TCCGCTATGA GAAGAAGAAG 
AAGGCTCTGA ATGATGTTCC CAGGTCAGCG GAGACCCACA AGGCACCACC ACCCCGCGAA 
GGAGTGGTGG AAGGGGCAGA GGCATGCCCC ATTGCTGAGG GCACCTTGGA GGAGAGACCC 
AAGGCTGCCA GCAAGCCAAG TCCTGAAGCT GTGGAAGAGC CCTTGGAAAA GCCCCAGCAG 
GTAGCAGTCA GTGAAGAGGT GAAGGAACGG AGTTGGCCCT TCTGGATGGG CAGCCCCCCA 
GACTCGGTGG TTACAGAGCT GAAGCGCAGC AAGGAGAAGG AGGCTGGGAC TCAGCAAAAA 
GTGGGAGCAG CAGCAGCAGC CCCTCAAGAG GAGAGCAACA CCAGTGCATC AGAGGGCAGC 
CAGACCATCA AGTGGGGACA CTTGTTTGGG TCCAGGAAGG CTCAGAAAGA GCCCAGGCAG 
CCTAACAGGT TGCCCTCTGG CTGGCTGAGT TTGGACAAGT CCGTGTTCCA GCTGGTGGCC 
CAGACGGTTG GGGCAAGCAT GTGGCGAGAG GCAGCTCCTG AGCCAGAACG CCCCCAGCCA 
GCGTCACCTG AAGTGCCCCC CATAGGGCAT CCAGCCCGGG GGCTGTCTCC TCACCCTCCC 
TGTGAGGTGA AGGCTCTTTG CCATCACATT GCCACCGAGG CAGGACAACT GAGCTTCAAC 
AAAGGGGATA TCTTGCAAGT CATCTCCAAA GTGGATGGTG ACTGGCTCCA GTGCAGCCTC 
GGCTCTGAGA AGGGACTGGT GCCCATCATG TACGTCACCC ACCCGGAGGA CGAGGACTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808006.1
CDS	589..5331
Translation	MDSPPKLTGETLIVHHIPLVHCQVPDRQCCSVSKRTNPFCQPELSITRNSALPDRDLSQTDSLVYSSFLQTSETSAEASDNKEGKAKDLIVPSVSKRHNPFLLSEGEDLSIFGDDLGQKSFHLHNSLVAGKPPFQLHELALPPFHLHDSNHIVKSWNMASRSGVVDGQEDKISSDDIQKRNNANRCHQASERMELDECSCHRGSSSAFSFDGGDQEWNQNTGELLRNQDALRSRTCSCSSSELQHCRCYSSSSQSEVIDQQMGYISDSSCNSSDGVLVNFSALYNKMNGHSRSNLNSANLSCDSSFCSHSDTGAFYLDLHSSPTESKMSCESHHPENSEKVCGCQHSSSPVLDANCNSYHLHCEPCASESSDLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEDHTKGSPAQPTEYYLFRRPDLRQEEDNVECSEEEAKGEATQNMIEGQVYVNVSPPNLNTSRQRSRSYDQNLDRSPSSRLGSLERMVSCPVKLSESPAIPIQSSPPKRVTSFAELAKGRKKNGTSPPLRSSGDSSLEFSPIPETQRDCPTFLEERARRSQSLPPMPFVHGLNRSCDGFCLNHTFGDSQAACSTKDSVSSEKVPSGHGAGGGTDGKPVVRYSKDQRPTTLPIQPFVFQHHFSKPPKARALHSHFASSLSQLYSLSSNRPASQQISSNSQSSAPATAAEQPAAVGSQAHSTLLTQRSVDGAASRNDGCVKKAAPETTRPSPLGSYSPVRCNVPFFQSVDSSSSPTAERAGESQPPRSRSCPISASLLPTRSSPAVSAVQPSQATKADALKQKENPKPVPKKEAPLEAGPPLSEYRLHSVSLPPLSVGGMPMSRVGGHADPHWRSGSETSSSGPLNSMGTRPLNANHLSPQALKWREYRRRNPLGLDRVSGLPGLASSLDRRQQEPRLNRGNTIFELPGSLNTSHFHCKLNGQSMRQLQLTYTDFFPDYFSLAEKPPAEFCLSPDGNTESISIDLLQKKGLVKAINTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLILKYLCPAVRDILSDGLKAYVLDMIIGQRRNIPWSVVEASTQLGPSTKLLHSLYTKISQYTELTNHTMRFNAFLFGLLNIRSLEFWFNHLYNHEDIILAHYQPVGFLCLSHSVCQPLFEELLLLLQPLSLLPFNLDLLFEHHLMQMGKEQQQQKELLRVKQDLLLSAHSTLQLMRTRGSSDDPDGCSTAPEAGKVGTTDGDISPGHSSQQAASERVKGVGASCGDVEKDEERRKVRESTWEGKKDKQAGWWYQLMQSSQIYIEGSPEGSKFIRYEKKKKALNDVPRSAETHKAPPPREGVVEGAEACPIAEGTLEERPKAASKPSPEAVEEPLEKPQQVAVSEEVKERSWPFWMGSPPDSVVTELKRSKEKEAGTQQKVGAAAAAPQEESNTSASEGSQTIKWGHLFGSRKAQKEPRQPNRLPSGWLSLDKSVFQLVAQTVGASMWREAAPEPERPQPASPEVPPIGHPARGLSPHPPCEVKALCHHIATEAGQLSFNKGDILQVISKVDGDWLQCSLGSEKGLVPIMYVTHPEDEDY

Target ORF information:

RefSeq Version	XM_014952520.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952520.1

ORF Insert Sequence:

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGGTGGCAC AGACGGCAAG CCCGTGGTAC GCTACAGCAA GGACCAGCGT 
CCCACGACTC TGCCCATCCA GCCCTTTGTT TTCCAGCACC ACTTCAGCAA GCCACCCAAG 
GCCCGTGCCC TGCACAGCCA TTTTGCCTCC AGCCTTTCCC AACTCTACAG CTTGTCCAGC 
AACCGACCTG CCAGTCAGCA GATCTCCTCC AATTCCCAGT CCTCGGCCCC GGCCACTGCG 
GCAGAGCAGC CGGCGGCGGT GGGGAGCCAG GCCCACAGCA CGCTCCTGAC CCAACGCTCG 
GTGGATGGAG CTGCCTCCCG CAACGATGGC TGCGTTAAGA AGGCTGCCCC GGAGACAACC 
CGGCCGTCCC CCCTGGGGAG TTACTCTCCT GTGCGATGCA ACGTGCCTTT CTTCCAAAGC 
GTGGACTCTT CTTCCTCGCC CACCGCCGAG AGAGCTGGAG AGAGCCAGCC CCCCAGGAGC 
AGGTCCTGCC CCATCTCCGC CAGCCTGCTT CCCACCAGGT CTTCCCCTGC GGTCAGTGCT 
GTGCAGCCCT CCCAAGCCAC CAAAGCTGAT GCCCTGAAGC AGAAGGAGAA CCCCAAGCCG 
GTTCCCAAGA AGGAGGCACC GCTGGAAGCA GGCCCACCAC TCTCTGAGTA CCGACTCCAC 
AGTGTGTCCC TCCCACCGCT CTCGGTGGGT GGCATGCCCA TGAGCAGAGT GGGAGGCCAC 
GCAGATCCCC ACTGGAGAAG TGGCAGTGAG ACCAGCAGCT CTGGTCCCCT GAACAGCATG 
GGAACACGGC CCCTCAATGC GAACCACCTC TCCCCCCAAG CCCTGAAGTG GAGGGAGTAC 
AGGCGGAGGA ACCCCCTGGG TCTGGACCGT GTTTCGGGGC TGCCTGGCTT AGCAAGCAGC 
CTAGACAGGA GGCAGCAAGA GCCCCGGCTG AACCGGGGGA ACACCATCTT TGAGCTCCCC 
GGCAGCCTCA ACACCAGCCA TTTCCACTGC AAGCTGAACG GACAGTCAAT GAGGCAACTC 
CAGCTTACCT ACACCGACTT CTTCCCCGAC TACTTCTCAC TAGCAGAGAA ACCCCCAGCT 
GAGTTCTGCC TCTCCCCAGA TGGCAACACA GAGTCTATCT CCATCGACTT GCTACAGAAG 
AAGGGTCTGG TGAAGGCCAT CAACACTGCT GTTGACCTGA TTGTGGCTCA CTTTGGAACC 
AGCAGGGATC CAGGAGTGAA GGCCAAACTT GGGAACAGCT CTGTGAGCCC CAACGTGGGG 
CATCTCATCC TGAAATACCT ATGCCCTGCT GTCCGGGACA TCCTGAGTGA CGGGCTTAAG 
GCTTACGTCC TGGACATGAT CATTGGCCAA AGGAGGAACA TCCCCTGGAG CGTGGTGGAG 
GCATCGACTC AGCTTGGCCC CTCCACCAAG CTGCTGCACA GCCTCTATAC CAAGATCAGC 
CAGTACACAG AGCTCACCAA CCACACCATG AGGTTCAACG CGTTCCTCTT TGGCCTCCTC 
AATATCCGGT CCTTGGAGTT CTGGTTCAAC CACCTCTACA ACCATGAAGA TATCATCCTG 
GCACACTACC AGCCGGTGGG CTTCTTGTGC CTGTCTCACA GCGTGTGCCA GCCTCTTTTC 
GAGGAGCTCC TGCTCCTGCT GCAGCCGCTC TCCCTGCTGC CCTTCAACCT GGACCTCCTT 
TTCGAGCACC ACCTGATGCA GATGGGCAAA GAGCAGCAGC AGCAAAAGGA GCTGCTGCGT 
GTGAAGCAGG ACCTGCTGCT GTCCGCCCAC TCCACCCTGC AGCTGATGCG GACACGGGGC 
AGCAGCGACG ATCCCGACGG CTGCAGCACC GCTCCCGAAG CAGGCAAAGT GGGCACCACA 
GATGGTGACA TCTCTCCAGG CCACAGCTCC CAGCAAGCTG CCAGCGAGAG GGTGAAGGGG 
GTGGGGGCCT CCTGTGGAGA TGTGGAGAAG GATGAAGAGC GGAGGAAGGT GCGGGAGAGC 
ACGTGGGAGG GGAAGAAGGA CAAGCAGGCG GGCTGGTGGT ACCAGCTCAT GCAGAGCTCT 
CAGATTTACA TCGAGGGCTC ACCCGAAGGC TCGAAGTTCA TCCGCTATGA GAAGAAGAAG 
AAGGCTCTGA ATGATGTTCC CAGGTCAGCG GAGACCCACA AGGCACCACC ACCCCGCGAA 
GGAGTGGTGG AAGGGGCAGA GGCATGCCCC ATTGCTGAGG GCACCTTGGA GGAGAGACCC 
AAGGCTGCCA GCAAGCCAAG TCCTGAAGCT GTGGAAGAGC CCTTGGAAAA GCCCCAGCAG 
GTAGCAGTCA GTGAAGAGGT GAAGGAACGG AGTTGGCCCT TCTGGATGGG CAGCCCCCCA 
GACTCGGTGG TTACAGAGCT GAAGCGCAGC AAGGAGAAGG AGGCTGGGAC TCAGCAAAAA 
GTGGGAGCAG CAGCAGCAGC CCCTCAAGAG GAGAGCAACA CCAGTGCATC AGAGGGCAGC 
CAGACCATCA AGTGGGGACA CTTGTTTGGG TCCAGGAAGG CTCAGAAAGA GCCCAGGCAG 
CCTAACAGGT TGCCCTCTGG CTGGCTGAGT TTGGACAAGT CCGTGTTCCA GCTGGTGGCC 
CAGACGGTTG GGGCAAGCAT GTGGCGAGAG GCAGCTCCTG AGCCAGAACG CCCCCAGCCA 
GCGTCACCTG AAGTGCCCCC CATAGGGCAT CCAGCCCGGG GGCTGTCTCC TCACCCTCCC 
TGTGAGGTGA AGGCTCTTTG CCATCACATT GCCACCGAGG CAGGACAACT GAGCTTCAAC 
AAAGGGGATA TCTTGCAAGT CATCTCCAAA GTGGATGGTG ACTGGCTCCA GTGCAGCCTC 
GGCTCTGAGA AGGGACTGGT GCCCATCATG TACGTCACCC ACCCGGAGGA CGAGGACTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUSC2 ( XM_014952521.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808007.1 Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCa72711
Clone ID Related Accession (Same CDS sequence)	XM_014952521.1
Accession Version	XM_014952521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	iporin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090963.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GGACAGTCAA TGAGGCAACT CCAGCTTACC TACACCGACT TCTTCCCCGA CTACTTCTCA 
CTAGCAGAGA AACCCCCAGC TGAGTTCTGC CTCTCCCCAG ATGGCAACAC AGAGTCTATC 
TCCATCGACT TGCTACAGAA GAAGGGTCTG GTGAAGGCCA TCAACACTGC TGTTGACCTG 
ATTGTGGCTC ACTTTGGAAC CAGCAGGGAT CCAGGAGTGA AGGCCAAACT TGGGAACAGC 
TCTGTGAGCC CCAACGTGGG GCATCTCATC CTGAAATACC TATGCCCTGC TGTCCGGGAC 
ATCCTGAGTG ACGGGCTTAA GGCTTACGTC CTGGACATGA TCATTGGCCA AAGGAGGAAC 
ATCCCCTGGA GCGTGGTGGA GGCATCGACT CAGCTTGGCC CCTCCACCAA GCTGCTGCAC 
AGCCTCTATA CCAAGATCAG CCAGTACACA GAGCTCACCA ACCACACCAT GAGGTTCAAC 
GCGTTCCTCT TTGGCCTCCT CAATATCCGG TCCTTGGAGT TCTGGTTCAA CCACCTCTAC 
AACCATGAAG ATATCATCCT GGCACACTAC CAGCCGGTGG GCTTCTTGTG CCTGTCTCAC 
AGCGTGTGCC AGCCTCTTTT CGAGGAGCTC CTGCTCCTGC TGCAGCCGCT CTCCCTGCTG 
CCCTTCAACC TGGACCTCCT TTTCGAGCAC CACCTGATGC AGATGGGCAA AGAGCAGCAG 
CAGCAAAAGG AGCTGCTGCG TGTGAAGCAG GACCTGCTGC TGTCCGCCCA CTCCACCCTG 
CAGCTGATGC GGACACGGGG CAGCAGCGAC GATCCCGACG GCTGCAGCAC CGCTCCCGAA 
GCAGGCAAAG TGGGCACCAC AGATGGTGAC ATCTCTCCAG GCCACAGCTC CCAGCAAGCT 
GCCAGCGAGA GGGTGAAGGG GGTGGGGGCC TCCTGTGGAG ATGTGGAGAA GGATGAAGAG 
CGGAGGAAGG TGCGGGAGAG CACGTGGGAG GGGAAGAAGG ACAAGCAGGC GGGCTGGTGG 
TACCAGCTCA TGCAGAGCTC TCAGATTTAC ATCGAGGGCT CACCCGAAGG CTCGAAGTTC 
ATCCGCTATG AGAAGAAGAA GAAGGCTCTG AATGATGTTC CCAGGTCAGC GGAGACCCAC 
AAGGCACCAC CACCCCGCGA AGGAGTGGTG GAAGGGGCAG AGGCATGCCC CATTGCTGAG 
GGCACCTTGG AGGAGAGACC CAAGGCTGCC AGCAAGCCAA GTCCTGAAGC TGTGGAAGAG 
CCCTTGGAAA AGCCCCAGCA GGTAGCAGTC AGTGAAGAGG TGAAGGAACG GAGTTGGCCC 
TTCTGGATGG GCAGCCCCCC AGACTCGGTG GTTACAGAGC TGAAGCGCAG CAAGGAGAAG 
GAGGCTGGGA CTCAGCAAAA AGTGGGAGCA GCAGCAGCAG CCCCTCAAGA GGAGAGCAAC 
ACCAGTGCAT CAGAGGGCAG CCAGACCATC AAGTGGGGAC ACTTGTTTGG GTCCAGGAAG 
GCTCAGAAAG AGCCCAGGCA GCCTAACAGG TTGCCCTCTG GCTGGCTGAG TTTGGACAAG 
TCCGTGTTCC AGCTGGTGGC CCAGACGGTT GGGGCAAGCA TGTGGCGAGA GGCAGCTCCT 
GAGCCAGAAC GCCCCCAGCC AGCGTCACCT GAAGTGCCCC CCATAGGGCA TCCAGCCCGG 
GGGCTGTCTC CTCACCCTCC CTGTGAGGTG AAGGCTCTTT GCCATCACAT TGCCACCGAG 
GCAGGACAAC TGAGCTTCAA CAAAGGGGAT ATCTTGCAAG TCATCTCCAA AGTGGATGGT 
GACTGGCTCC AGTGCAGCCT CGGCTCTGAG AAGGGACTGG TGCCCATCAT GTACGTCACC 
CACCCGGAGG ACGAGGACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808007.1
CDS	590..5233
Translation	MDSPPKLTGETLIVHHIPLVHCQVPDRQCCSVSKRTNPFCQPELSITRNSALPDRDLSQTDSLVYSSFLQTSETSAEASDNKEGKAKDLIVPSVSKRHNPFLLSEGEDLSIFGDDLGQKSFHLHNSLVAGKPPFQLHELALPPFHLHDSNHIVKSWNMASRSGVVDGQEDKISSDDIQKRNNANRCHQASERMELDECSCHRGSSSAFSFDGGDQEWNQNTGELLRNQDALRSRTCSCSSSELQHCRCYSSSSQSEVIDQQMGYISDSSCNSSDGVLVNFSALYNKMNGHSRSNLNSANLSCDSSFCSHSDTGAFYLDLHSSPTESKMSCESHHPENSEKVCGCQHSSSPVLDANCNSYHLHCEPCASESSDLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEDHTKGSPAQPTEYYLFRRPDLRQEEDNVECSEEEAKGEATQNMIEGQVYVNVSPPNLNTSRQRSRSYDQNLDRSPSSRLGSLERMVSCPVKLSESPAIPIQSSPPKRVTSFAELAKGRKKNGTSPPLRSSGDSSLEFSPIPETQRDCPTFLEERARRSQSLPPMPFVHGLNRSCDGFCLNHTFGDSQAACSTKDSVSSEKVPSGHGAGEQASLSLLMEADASFSGGSASGHGQRDVRARADGGGTDGKPVVRYSKDQRPTTLPIQPFVFQHHFSKPPKARALHSHFASSLSQLYSLSSNRPASQQISSNSQSSAPATAAEQPAAVGSQAHSTLLTQRSVDGAASRNDGCVKKAAPETTRPSPLGSYSPVRCNVPFFQSVDSSSSPTAERAGESQPPRSRSCPISASLLPTRSSPAVSAVQPSQATKADALKQKENPKPVPKKEAPLEAGPPLSEYRLHSVSLPPLSVGGMPMSRVGGHADPHWRSGSETSSSGPLNSMGTRPLNGQSMRQLQLTYTDFFPDYFSLAEKPPAEFCLSPDGNTESISIDLLQKKGLVKAINTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLILKYLCPAVRDILSDGLKAYVLDMIIGQRRNIPWSVVEASTQLGPSTKLLHSLYTKISQYTELTNHTMRFNAFLFGLLNIRSLEFWFNHLYNHEDIILAHYQPVGFLCLSHSVCQPLFEELLLLLQPLSLLPFNLDLLFEHHLMQMGKEQQQQKELLRVKQDLLLSAHSTLQLMRTRGSSDDPDGCSTAPEAGKVGTTDGDISPGHSSQQAASERVKGVGASCGDVEKDEERRKVRESTWEGKKDKQAGWWYQLMQSSQIYIEGSPEGSKFIRYEKKKKALNDVPRSAETHKAPPPREGVVEGAEACPIAEGTLEERPKAASKPSPEAVEEPLEKPQQVAVSEEVKERSWPFWMGSPPDSVVTELKRSKEKEAGTQQKVGAAAAAPQEESNTSASEGSQTIKWGHLFGSRKAQKEPRQPNRLPSGWLSLDKSVFQLVAQTVGASMWREAAPEPERPQPASPEVPPIGHPARGLSPHPPCEVKALCHHIATEAGQLSFNKGDILQVISKVDGDWLQCSLGSEKGLVPIMYVTHPEDEDY

Target ORF information:

RefSeq Version	XM_014952521.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RUN and SH3 domain containing 2 (RUSC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952521.1

ORF Insert Sequence:

ATGGATAGTC CGCCCAAACT GACTGGTGAG ACGCTGATTG TCCATCACAT TCCCCTGGTG 
CATTGCCAGG TCCCTGATAG ACAGTGCTGC TCCGTGAGCA AAAGGACCAA CCCCTTCTGC 
CAGCCAGAGC TCAGCATTAC ACGGAACTCT GCCCTTCCAG ACAGAGACCT TTCACAGACT 
GACTCCTTGG TGTACAGCAG CTTTCTCCAG ACCTCTGAAA CCTCAGCAGA GGCCTCAGAC 
AACAAAGAAG GCAAAGCGAA AGATTTGATT GTCCCTAGTG TCAGTAAGCG ACACAACCCT 
TTCCTCCTGA GTGAGGGTGA AGACCTCAGC ATCTTTGGGG ATGACTTGGG TCAAAAATCT 
TTCCACCTTC ACAACTCACT TGTGGCTGGC AAACCTCCCT TTCAGCTGCA CGAGCTGGCC 
TTACCCCCTT TCCACCTCCA CGACTCCAAC CACATTGTGA AATCCTGGAA CATGGCCAGC 
CGGTCCGGTG TGGTAGATGG GCAAGAGGAC AAAATCAGCA GTGATGACAT CCAGAAGAGG 
AACAATGCAA ACAGGTGCCA CCAGGCCTCA GAACGCATGG AGCTGGATGA ATGCAGCTGC 
CATCGCGGCA GCTCCTCCGC CTTCTCCTTC GATGGTGGTG ACCAAGAGTG GAACCAGAAC 
ACAGGTGAAT TGCTGAGGAA TCAGGATGCC CTGCGCAGCC GGACATGCAG CTGTTCCAGC 
TCGGAGCTCC AGCACTGTCG CTGCTACAGT TCATCAAGTC AGTCTGAAGT CATTGACCAA 
CAGATGGGCT ACATCAGCGA CTCTTCCTGC AACAGCTCTG ATGGGGTGCT GGTGAACTTC 
AGTGCTCTCT ACAACAAAAT GAATGGTCAT TCTCGATCTA ACCTGAATTC AGCCAACCTG 
TCCTGTGACT CTTCCTTCTG CAGCCACTCA GACACAGGAG CTTTTTACCT GGATTTGCAT 
TCATCACCCA CAGAATCCAA GATGTCTTGC GAGTCGCATC ACCCAGAGAA TTCAGAGAAG 
GTGTGTGGGT GTCAGCACTC CTCCTCACCT GTCCTTGATG CTAACTGCAA CTCCTACCAC 
CTCCACTGTG AGCCTTGCGC TTCGGAGAGC TCAGACCTCA CTGCTTGCTT CCAGAGCCAA 
GCACGGCTCG TTGTGGCTAC TCAGAATTAT TATAAGTTAG TCACGTGTGA CTTGTCTTCC 
CAGTCATCCC CCAGCCCAGC AGGATCTTCC ATAACTAGCT GCTCGGAAGA CCATACCAAA 
GGTAGCCCCG CCCAGCCCAC TGAATATTAT CTGTTTAGAA GGCCTGACCT GAGACAAGAA 
GAAGATAATG TGGAGTGCAG TGAAGAGGAG GCAAAGGGAG AAGCCACACA GAACATGATT 
GAAGGTCAGG TGTATGTGAA TGTGTCACCA CCTAACCTCA ACACAAGCCG TCAGCGCTCC 
AGGAGCTATG ATCAGAACCT AGACAGGAGT CCCAGCAGCC GGCTGGGCTC CTTGGAGCGC 
ATGGTGAGCT GTCCGGTCAA GCTGAGTGAA AGTCCAGCAA TACCCATCCA GAGTTCCCCC 
CCAAAGCGAG TGACATCTTT TGCTGAACTA GCTAAAGGCC GGAAAAAGAA TGGCACCTCC 
CCACCGCTTC GGTCCAGTGG TGATTCCTCC TTGGAGTTCT CCCCCATCCC TGAGACACAA 
CGGGATTGCC CAACCTTCCT TGAAGAGAGA GCTCGCCGCA GCCAGAGTCT TCCACCCATG 
CCCTTTGTCC ATGGCCTGAA CCGGAGCTGT GATGGCTTCT GTTTGAATCA CACTTTTGGG 
GACAGCCAGG CTGCGTGTTC CACCAAAGAC TCGGTCTCCA GTGAGAAGGT CCCCAGTGGG 
CATGGGGCAG GTGAGCAAGC CTCTCTCTCC CTGCTGATGG AGGCAGATGC CAGCTTCTCA 
GGTGGCTCTG CCAGTGGCCA CGGACAAAGA GATGTTAGAG CCCGAGCAGA CGGTGGTGGC 
ACAGACGGCA AGCCCGTGGT ACGCTACAGC AAGGACCAGC GTCCCACGAC TCTGCCCATC 
CAGCCCTTTG TTTTCCAGCA CCACTTCAGC AAGCCACCCA AGGCCCGTGC CCTGCACAGC 
CATTTTGCCT CCAGCCTTTC CCAACTCTAC AGCTTGTCCA GCAACCGACC TGCCAGTCAG 
CAGATCTCCT CCAATTCCCA GTCCTCGGCC CCGGCCACTG CGGCAGAGCA GCCGGCGGCG 
GTGGGGAGCC AGGCCCACAG CACGCTCCTG ACCCAACGCT CGGTGGATGG AGCTGCCTCC 
CGCAACGATG GCTGCGTTAA GAAGGCTGCC CCGGAGACAA CCCGGCCGTC CCCCCTGGGG 
AGTTACTCTC CTGTGCGATG CAACGTGCCT TTCTTCCAAA GCGTGGACTC TTCTTCCTCG 
CCCACCGCCG AGAGAGCTGG AGAGAGCCAG CCCCCCAGGA GCAGGTCCTG CCCCATCTCC 
GCCAGCCTGC TTCCCACCAG GTCTTCCCCT GCGGTCAGTG CTGTGCAGCC CTCCCAAGCC 
ACCAAAGCTG ATGCCCTGAA GCAGAAGGAG AACCCCAAGC CGGTTCCCAA GAAGGAGGCA 
CCGCTGGAAG CAGGCCCACC ACTCTCTGAG TACCGACTCC ACAGTGTGTC CCTCCCACCG 
CTCTCGGTGG GTGGCATGCC CATGAGCAGA GTGGGAGGCC ACGCAGATCC CCACTGGAGA 
AGTGGCAGTG AGACCAGCAG CTCTGGTCCC CTGAACAGCA TGGGAACACG GCCCCTCAAT 
GGACAGTCAA TGAGGCAACT CCAGCTTACC TACACCGACT TCTTCCCCGA CTACTTCTCA 
CTAGCAGAGA AACCCCCAGC TGAGTTCTGC CTCTCCCCAG ATGGCAACAC AGAGTCTATC 
TCCATCGACT TGCTACAGAA GAAGGGTCTG GTGAAGGCCA TCAACACTGC TGTTGACCTG 
ATTGTGGCTC ACTTTGGAAC CAGCAGGGAT CCAGGAGTGA AGGCCAAACT TGGGAACAGC 
TCTGTGAGCC CCAACGTGGG GCATCTCATC CTGAAATACC TATGCCCTGC TGTCCGGGAC 
ATCCTGAGTG ACGGGCTTAA GGCTTACGTC CTGGACATGA TCATTGGCCA AAGGAGGAAC 
ATCCCCTGGA GCGTGGTGGA GGCATCGACT CAGCTTGGCC CCTCCACCAA GCTGCTGCAC 
AGCCTCTATA CCAAGATCAG CCAGTACACA GAGCTCACCA ACCACACCAT GAGGTTCAAC 
GCGTTCCTCT TTGGCCTCCT CAATATCCGG TCCTTGGAGT TCTGGTTCAA CCACCTCTAC 
AACCATGAAG ATATCATCCT GGCACACTAC CAGCCGGTGG GCTTCTTGTG CCTGTCTCAC 
AGCGTGTGCC AGCCTCTTTT CGAGGAGCTC CTGCTCCTGC TGCAGCCGCT CTCCCTGCTG 
CCCTTCAACC TGGACCTCCT TTTCGAGCAC CACCTGATGC AGATGGGCAA AGAGCAGCAG 
CAGCAAAAGG AGCTGCTGCG TGTGAAGCAG GACCTGCTGC TGTCCGCCCA CTCCACCCTG 
CAGCTGATGC GGACACGGGG CAGCAGCGAC GATCCCGACG GCTGCAGCAC CGCTCCCGAA 
GCAGGCAAAG TGGGCACCAC AGATGGTGAC ATCTCTCCAG GCCACAGCTC CCAGCAAGCT 
GCCAGCGAGA GGGTGAAGGG GGTGGGGGCC TCCTGTGGAG ATGTGGAGAA GGATGAAGAG 
CGGAGGAAGG TGCGGGAGAG CACGTGGGAG GGGAAGAAGG ACAAGCAGGC GGGCTGGTGG 
TACCAGCTCA TGCAGAGCTC TCAGATTTAC ATCGAGGGCT CACCCGAAGG CTCGAAGTTC 
ATCCGCTATG AGAAGAAGAA GAAGGCTCTG AATGATGTTC CCAGGTCAGC GGAGACCCAC 
AAGGCACCAC CACCCCGCGA AGGAGTGGTG GAAGGGGCAG AGGCATGCCC CATTGCTGAG 
GGCACCTTGG AGGAGAGACC CAAGGCTGCC AGCAAGCCAA GTCCTGAAGC TGTGGAAGAG 
CCCTTGGAAA AGCCCCAGCA GGTAGCAGTC AGTGAAGAGG TGAAGGAACG GAGTTGGCCC 
TTCTGGATGG GCAGCCCCCC AGACTCGGTG GTTACAGAGC TGAAGCGCAG CAAGGAGAAG 
GAGGCTGGGA CTCAGCAAAA AGTGGGAGCA GCAGCAGCAG CCCCTCAAGA GGAGAGCAAC 
ACCAGTGCAT CAGAGGGCAG CCAGACCATC AAGTGGGGAC ACTTGTTTGG GTCCAGGAAG 
GCTCAGAAAG AGCCCAGGCA GCCTAACAGG TTGCCCTCTG GCTGGCTGAG TTTGGACAAG 
TCCGTGTTCC AGCTGGTGGC CCAGACGGTT GGGGCAAGCA TGTGGCGAGA GGCAGCTCCT 
GAGCCAGAAC GCCCCCAGCC AGCGTCACCT GAAGTGCCCC CCATAGGGCA TCCAGCCCGG 
GGGCTGTCTC CTCACCCTCC CTGTGAGGTG AAGGCTCTTT GCCATCACAT TGCCACCGAG 
GCAGGACAAC TGAGCTTCAA CAAAGGGGAT ATCTTGCAAG TCATCTCCAA AGTGGATGGT 
GACTGGCTCC AGTGCAGCCT CGGCTCTGAG AAGGGACTGG TGCCCATCAT GTACGTCACC 
CACCCGGAGG ACGAGGACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RUSC2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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