MED15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED15
Entrez Gene ID	33223
Full Name	Mediator complex subunit 15
Synonyms	Arc105,BcDNA:GH03922,CG4184,Dmel\CG4184,Med15,Med9/ARC105,dARC105,med15
General protein information	Preferred Names Mediator complex subunit 15 Names CG4184 gene product from transcript CG4184-RA CG4184-PA MED15-PA mediator complex subunit 15 mediator15
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21D1-21D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_134684.3	NP_608528.1	mediator complex subunit 15

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13354	NM_134684.3 Latest version!	Drosophila melanogaster mediator complex subunit 15 (MED15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED15 ( NM_134684.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608528.1 Drosophila melanogaster mediator complex subunit 15 (MED15), mRNA.

Price & Availability↑

CloneID	OFa13354
Clone ID Related Accession (Same CDS sequence)	NM_134684.3
Accession Version	NM_134684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134684.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAGG ACTGGCAGAG TCAAAAGTTT CGTCAGAATG TCATCTCCAA AATCCACGAC 
TTATTGCCAC CGAATGCGCA GGACCAGACT AAAAATGCCG GCGTTATGGA GAACCACATA 
TTTCGAAAGT CGCGTACTAA GGACGAGTAT TTAGGTCTGG TAGCCAAGCT CTTTATGCAT 
TACAAAGACA TGTCACGAAA GTCCCAGCAA CAGCAGCAGC AGCAACAACA GCAGGGTGGC 
CCGCCCCCAA ATGCGGAAAT GGGCGGCGGG CAGAATATGA TGCAGGATCC ACTGAACGCA 
CTTCAGAATC TTGCCAGTCA AGGAAATCGC AATCCCCAGA TGATGCCCAT GGGCGCCGGA 
GGAGGAGCGC CAGTGCCCGG TGGCCCCGGA ACTGCCTCTA ACTTGCTACA ATCCCTGAAT 
CAGCAGCGCC CTGGACAGCA GCAAATGCAG CCCATGTCAA ATATCCGTGG CCAAATGCCC 
ATGGGTGCCG GAGGAGCTGG TGCCCAGCAG ATGATGCAGG TTCAACAGAT GCAGCAAGGT 
GGGAATGCCC CCGGAGTGAT GAACGTAATG GGCGCCGGCG GCGGGCAAAA TCAGGGACAG 
ATTGTCGGTA ATCCCGGGCA GCAAATGGGC GTGGGCGTTG GCATGCCTAA CCAAATGGTA 
GGACCGGGAC CGAACAGTGG ACCCGCCGTT GGCGGAGCTG GCGGACCAAA TGCTGCTCCA 
GGAGCAGGCG GACCCGGGCC AAATCAGATG CAGGGCGGAC CAATGAACGT AAATGCCATG 
CAACAGATGC CTCCCATGCA GCAGATTCAG CAGAACCAAT TGGGGATGGG CATGAATCCC 
ATGATGCGGA TGGGCCAAGG CAACGGAATG GGCGGGCCAC AAGGAATGCC CGGCCAGGGT 
ATGCAGGGAA TGCCGCAGGG ACCACACAAT GTAGTTGGCG GACCTGCGGG TCAGCAACAA 
GTGGGTGGAG CAGGCCTGCC CCCCAACGCT GTGCAACAGG GAGGAATGAA CCCCATGGGC 
GGTATGGGCG TGAACATGCC ACCGAATTTG CAGCAAAAAC CAAATATGCC CATGGGCCAG 
GCGGGTCAGA TGTTTCCCGG TAATCGAGGA GGTGTGGGAG TGGGTGGACA GCAGCCGGGA 
CAGCCCTTCA TGCGATCCAG TCCCTCTCCA GCAGATGCTC AGCAATTACA GCAACAGGCG 
CAGCTTCAGC AAATGCAACA ACAGCAGCAA CAGCTGGTGG TGGGCAATCA GACGCCAACA 
CAGCAACCTC CGACTCCGCA GATGCCCACG CCCAATATGA TTCCTAGTCC GGCACTGGTT 
CCCCAGTCCA GTCCGCAGAT GATGCAGATG CAGAATTCGC AGCGCAATAT TCGGCAGCAG 
TCTCCAAGTG CCTCGATCAA CACTCCTGGC CAGGTTACTG GTAACAGTCC ATTTAATCCC 
CAGGAGGAGG CATTATACCG CGAGAAATAC AAGCAGCTGA CCAAGTACAT TGAGCCACTG 
AAGCGGATGC TTGCCAAGAT CAGTAACGAC GGAACCAATG TGGAGAAGAT GACAAAGATG 
AGCAAGTTGT TGGAGATTCT CTGCAATCCT ACACAGCGCG TGCCGCTGGA GACTCTACTT 
AAGTGTGAAA AAGCGCTTGA GAAGATGGAT CTCATCTCCT ATTCCGGCCA GCAGTTTGGT 
AAATCCTCAA ATCCATTGCT GGAGGTTATA AATACAACGC TGCAAAGTCC TGTAGCAAAT 
CACACATTGT ATCGCACATT CCGACCCACT TTGGAGCTGC TCTTTGGTAC GGATATAACA 
GCTCCAGTGC CCGCAAAGCG ACCTCGGGTG GAAGAAAAGT CCACGTCTTT TGAGCAGGAA 
GTGCCACATG TGCTTCAAGG GGAAATTGCT AGGCTCGATA CCAAGTTTAA AGTGAAATTG 
GACACCACGT CACAGATCAA CAACAAGGCC ATTCGCTTGA TTTGTTGCCT GGATGACAAA 
AGGTTGCCTA GTGTTCCGCC CGTAAGTGTG AGTGTGCCGG AGGAATATCC GTGGCAGGCT 
CCGGACTGCT CCTTGGCGGA ACAGGAGTAT TCAGCCACCC CCTTCCTGCA GACGGTGCAA 
CAAGCTCTCA TTGCCCGCAT CTCGAAGTTG CCCAAGAACT ACTCGCTCTC ACATTTGTTG 
GACACCTGGG AGATGGCCGT GCGACAGGCG TGTTCCCCGC AATCGAAACC CAGAGCAGTC 
TGTGAACTTT CCACTTTGCT GGGAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608528.1
CDS	101..2350
Translation	MTEDWQSQKFRQNVISKIHDLLPPNAQDQTKNAGVMENHIFRKSRTKDEYLGLVAKLFMHYKDMSRKSQQQQQQQQQQGGPPPNAEMGGGQNMMQDPLNALQNLASQGNRNPQMMPMGAGGGAPVPGGPGTASNLLQSLNQQRPGQQQMQPMSNIRGQMPMGAGGAGAQQMMQVQQMQQGGNAPGVMNVMGAGGGQNQGQIVGNPGQQMGVGVGMPNQMVGPGPNSGPAVGGAGGPNAAPGAGGPGPNQMQGGPMNVNAMQQMPPMQQIQQNQLGMGMNPMMRMGQGNGMGGPQGMPGQGMQGMPQGPHNVVGGPAGQQQVGGAGLPPNAVQQGGMNPMGGMGVNMPPNLQQKPNMPMGQAGQMFPGNRGGVGVGGQQPGQPFMRSSPSPADAQQLQQQAQLQQMQQQQQQLVVGNQTPTQQPPTPQMPTPNMIPSPALVPQSSPQMMQMQNSQRNIRQQSPSASINTPGQVTGNSPFNPQEEALYREKYKQLTKYIEPLKRMLAKISNDGTNVEKMTKMSKLLEILCNPTQRVPLETLLKCEKALEKMDLISYSGQQFGKSSNPLLEVINTTLQSPVANHTLYRTFRPTLELLFGTDITAPVPAKRPRVEEKSTSFEQEVPHVLQGEIARLDTKFKVKLDTTSQINNKAIRLICCLDDKRLPSVPPVSVSVPEEYPWQAPDCSLAEQEYSATPFLQTVQQALIARISKLPKNYSLSHLLDTWEMAVRQACSPQSKPRAVCELSTLLGV

Target ORF information:

RefSeq Version	NM_134684.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 15 (MED15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134684.3

ORF Insert Sequence:

ATGACCGAGG ACTGGCAGAG TCAAAAGTTT CGTCAGAATG TCATCTCCAA AATCCACGAC 
TTATTGCCAC CGAATGCGCA GGACCAGACT AAAAATGCCG GCGTTATGGA GAACCACATA 
TTTCGAAAGT CGCGTACTAA GGACGAGTAT TTAGGTCTGG TAGCCAAGCT CTTTATGCAT 
TACAAAGACA TGTCACGAAA GTCCCAGCAA CAGCAGCAGC AGCAACAACA GCAGGGTGGC 
CCGCCCCCAA ATGCGGAAAT GGGCGGCGGG CAGAATATGA TGCAGGATCC ACTGAACGCA 
CTTCAGAATC TTGCCAGTCA AGGAAATCGC AATCCCCAGA TGATGCCCAT GGGCGCCGGA 
GGAGGAGCGC CAGTGCCCGG TGGCCCCGGA ACTGCCTCTA ACTTGCTACA ATCCCTGAAT 
CAGCAGCGCC CTGGACAGCA GCAAATGCAG CCCATGTCAA ATATCCGTGG CCAAATGCCC 
ATGGGTGCCG GAGGAGCTGG TGCCCAGCAG ATGATGCAGG TTCAACAGAT GCAGCAAGGT 
GGGAATGCCC CCGGAGTGAT GAACGTAATG GGCGCCGGCG GCGGGCAAAA TCAGGGACAG 
ATTGTCGGTA ATCCCGGGCA GCAAATGGGC GTGGGCGTTG GCATGCCTAA CCAAATGGTA 
GGACCGGGAC CGAACAGTGG ACCCGCCGTT GGCGGAGCTG GCGGACCAAA TGCTGCTCCA 
GGAGCAGGCG GACCCGGGCC AAATCAGATG CAGGGCGGAC CAATGAACGT AAATGCCATG 
CAACAGATGC CTCCCATGCA GCAGATTCAG CAGAACCAAT TGGGGATGGG CATGAATCCC 
ATGATGCGGA TGGGCCAAGG CAACGGAATG GGCGGGCCAC AAGGAATGCC CGGCCAGGGT 
ATGCAGGGAA TGCCGCAGGG ACCACACAAT GTAGTTGGCG GACCTGCGGG TCAGCAACAA 
GTGGGTGGAG CAGGCCTGCC CCCCAACGCT GTGCAACAGG GAGGAATGAA CCCCATGGGC 
GGTATGGGCG TGAACATGCC ACCGAATTTG CAGCAAAAAC CAAATATGCC CATGGGCCAG 
GCGGGTCAGA TGTTTCCCGG TAATCGAGGA GGTGTGGGAG TGGGTGGACA GCAGCCGGGA 
CAGCCCTTCA TGCGATCCAG TCCCTCTCCA GCAGATGCTC AGCAATTACA GCAACAGGCG 
CAGCTTCAGC AAATGCAACA ACAGCAGCAA CAGCTGGTGG TGGGCAATCA GACGCCAACA 
CAGCAACCTC CGACTCCGCA GATGCCCACG CCCAATATGA TTCCTAGTCC GGCACTGGTT 
CCCCAGTCCA GTCCGCAGAT GATGCAGATG CAGAATTCGC AGCGCAATAT TCGGCAGCAG 
TCTCCAAGTG CCTCGATCAA CACTCCTGGC CAGGTTACTG GTAACAGTCC ATTTAATCCC 
CAGGAGGAGG CATTATACCG CGAGAAATAC AAGCAGCTGA CCAAGTACAT TGAGCCACTG 
AAGCGGATGC TTGCCAAGAT CAGTAACGAC GGAACCAATG TGGAGAAGAT GACAAAGATG 
AGCAAGTTGT TGGAGATTCT CTGCAATCCT ACACAGCGCG TGCCGCTGGA GACTCTACTT 
AAGTGTGAAA AAGCGCTTGA GAAGATGGAT CTCATCTCCT ATTCCGGCCA GCAGTTTGGT 
AAATCCTCAA ATCCATTGCT GGAGGTTATA AATACAACGC TGCAAAGTCC TGTAGCAAAT 
CACACATTGT ATCGCACATT CCGACCCACT TTGGAGCTGC TCTTTGGTAC GGATATAACA 
GCTCCAGTGC CCGCAAAGCG ACCTCGGGTG GAAGAAAAGT CCACGTCTTT TGAGCAGGAA 
GTGCCACATG TGCTTCAAGG GGAAATTGCT AGGCTCGATA CCAAGTTTAA AGTGAAATTG 
GACACCACGT CACAGATCAA CAACAAGGCC ATTCGCTTGA TTTGTTGCCT GGATGACAAA 
AGGTTGCCTA GTGTTCCGCC CGTAAGTGTG AGTGTGCCGG AGGAATATCC GTGGCAGGCT 
CCGGACTGCT CCTTGGCGGA ACAGGAGTAT TCAGCCACCC CCTTCCTGCA GACGGTGCAA 
CAAGCTCTCA TTGCCCGCAT CTCGAAGTTG CCCAAGAACT ACTCGCTCTC ACATTTGTTG 
GACACCTGGG AGATGGCCGT GCGACAGGCG TGTTCCCCGC AATCGAAACC CAGAGCAGTC 
TGTGAACTTT CCACTTTGCT GGGAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MED15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

ORF Insert Sequence:

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