NC2alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NC2alpha
Entrez Gene ID	37471
Full Name	Negative Cofactor 2alpha
Synonyms	CG10318,Dmel\CG10318,Drap1,NC2,Nc2alpha,dDrap,dDrap1,dDrap1/dNC2a,dNC2
General protein information	Preferred Names Negative Cofactor 2alpha Names CG10318 gene product from transcript CG10318-RA CG10318-PA NC2alpha-PA negative cofactor 2alpha
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57F7-57F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_137757.3	NP_611601.2	negative cofactor 2alpha

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010678	XP_559178.3
Drosophila melanogaster (fruit fly)	NC2alpha	NP_611601.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TBP activates TATA-dependent transcription and represses DPE-dependent transcription, whereas Mot1 and NC2 block TBP function and thus repress TATA-dependent transcription and activate DPE-dependent transcription.
Title: TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription.

The following NC2alpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NC2alpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10837	NM_137757.3 Latest version!	Drosophila melanogaster negative cofactor 2alpha (NC2alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NC2alpha ( NM_137757.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611601.2 Drosophila melanogaster negative cofactor 2alpha (NC2alpha), mRNA.

Price & Availability↑

CloneID	OFa10837
Clone ID Related Accession (Same CDS sequence)	NM_137757.3
Accession Version	NM_137757.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	negative cofactor 2alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137757.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCATCGA AAAAGAAAAA ATACAACGCA CGCTTTCCGG CGGGTCGCAT CAAGAAGATC 
ATGCAAAGCG ACGAGGAAAT CGGGAAGGTG GCCCAGGCGG TGCCTGTTAT CATCTCACGC 
ACGCTGGAGC TCTTCGTGGA GTCGCTGCTG ACCAAGACGC TGCGCATCAC GAATGCGCGC 
AACGCCAAGA CGCTATCGCC ATCGCACATG AGGCAGTGTA TCGTATCCGA GAAACGCTTT 
GACTTCCTCA AGGAGCTGGT CCGCAACATA CCCGACATCA GTGTGGCCGA GGAGGCCGCC 
TACAACGAGG ACGATGTGTT ACGCAGCTCG CCAGAGGAAC AGTATCCAGA TTCAGACACG 
CCATATGATC TTAGCCTGCC ATCGACGTCA ATGCGCTCGC AGGCGAACGG CACAGCTGCC 
TACATGCGCT CCATGAGTCT GAACAACGGA GCCGGATCGG GCGGAGGAGC CGCAGCCACC 
AAGCGGCAGT TTCAGTCGCA GCACTCCACC CAGGAAACTC CCACCACCAG CACCACCCTG 
CCCGCCAAGC TGGCCCGCTC CGGGTCCATG CCGGCCTACA CGCCACGGGG CAGACCGCCC 
AATCACCTCA AGAAACAGTC GGTGCAGCTC GATACTGCCA TACCCGCCCC GATCTGCAAC 
TACGAACTCA ACAAGCCCAT TGTCAAGATC GACTACAGCC ACGTGCAAAT GCCGACGGCG 
AACCTGTGCA CACCGGACGC CTCTGATTCT GGGTCAGGAT CGGGCGCCGG ATCTGGAGCT 
TTTAACTTCG ACATAGCTGC TCCGGTGATC AATATCGATC TGACGAATAT CGTGGCCGGT 
GGAGCACCTG GCAGTGGTGT GCCCCCCGCC TCTATTGGAT TACCAGCGGC CACGCCGGGG 
GACAACAATA ATGTGAACCA AATGGCTGGA AAGAGCAGCG CTCCCATCAT TCCAAAGGCC 
ACAGCGGCAT CAGCGACCCC CACTGAAACC GTTTTCGAAT TAGACGAAGA CTATGACAAT 
ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611601.2
CDS	96..1121
Translation	MPSKKKKYNARFPAGRIKKIMQSDEEIGKVAQAVPVIISRTLELFVESLLTKTLRITNARNAKTLSPSHMRQCIVSEKRFDFLKELVRNIPDISVAEEAAYNEDDVLRSSPEEQYPDSDTPYDLSLPSTSMRSQANGTAAYMRSMSLNNGAGSGGGAAATKRQFQSQHSTQETPTTSTTLPAKLARSGSMPAYTPRGRPPNHLKKQSVQLDTAIPAPICNYELNKPIVKIDYSHVQMPTANLCTPDASDSGSGSGAGSGAFNFDIAAPVINIDLTNIVAGGAPGSGVPPASIGLPAATPGDNNNVNQMAGKSSAPIIPKATAASATPTETVFELDEDYDNI

Target ORF information:

RefSeq Version	NM_137757.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster negative cofactor 2alpha (NC2alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137757.3

ORF Insert Sequence:

ATGCCATCGA AAAAGAAAAA ATACAACGCA CGCTTTCCGG CGGGTCGCAT CAAGAAGATC 
ATGCAAAGCG ACGAGGAAAT CGGGAAGGTG GCCCAGGCGG TGCCTGTTAT CATCTCACGC 
ACGCTGGAGC TCTTCGTGGA GTCGCTGCTG ACCAAGACGC TGCGCATCAC GAATGCGCGC 
AACGCCAAGA CGCTATCGCC ATCGCACATG AGGCAGTGTA TCGTATCCGA GAAACGCTTT 
GACTTCCTCA AGGAGCTGGT CCGCAACATA CCCGACATCA GTGTGGCCGA GGAGGCCGCC 
TACAACGAGG ACGATGTGTT ACGCAGCTCG CCAGAGGAAC AGTATCCAGA TTCAGACACG 
CCATATGATC TTAGCCTGCC ATCGACGTCA ATGCGCTCGC AGGCGAACGG CACAGCTGCC 
TACATGCGCT CCATGAGTCT GAACAACGGA GCCGGATCGG GCGGAGGAGC CGCAGCCACC 
AAGCGGCAGT TTCAGTCGCA GCACTCCACC CAGGAAACTC CCACCACCAG CACCACCCTG 
CCCGCCAAGC TGGCCCGCTC CGGGTCCATG CCGGCCTACA CGCCACGGGG CAGACCGCCC 
AATCACCTCA AGAAACAGTC GGTGCAGCTC GATACTGCCA TACCCGCCCC GATCTGCAAC 
TACGAACTCA ACAAGCCCAT TGTCAAGATC GACTACAGCC ACGTGCAAAT GCCGACGGCG 
AACCTGTGCA CACCGGACGC CTCTGATTCT GGGTCAGGAT CGGGCGCCGG ATCTGGAGCT 
TTTAACTTCG ACATAGCTGC TCCGGTGATC AATATCGATC TGACGAATAT CGTGGCCGGT 
GGAGCACCTG GCAGTGGTGT GCCCCCCGCC TCTATTGGAT TACCAGCGGC CACGCCGGGG 
GACAACAATA ATGTGAACCA AATGGCTGGA AAGAGCAGCG CTCCCATCAT TCCAAAGGCC 
ACAGCGGCAT CAGCGACCCC CACTGAAACC GTTTTCGAAT TAGACGAAGA CTATGACAAT 
ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NC2alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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