Brca2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brca2
Entrez Gene ID	37916
Full Name	Breast cancer 2, early onset homolog
Synonyms	30169,BRCA2,BcDNA:SD25109,CG13583,CG13584,CG30169,Dmbrca2,Dmel\CG30169,brca2,dmbrca2
General protein information	Preferred Names Breast cancer 2, early onset homolog Names CG30169 gene product from transcript CG30169-RA Brca2-PA CG30169-PA breast cancer 2 early onset homolog breast cancer 2, early onset homolog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60D4-60D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_138081.4	NP_611925.2	BRCA2, DNA repair associated

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

persistent meiotic DNA double-strand breaks might correspond to crossovers, which are mobilized to the nuclear envelope for their repair; Brca2-Pds5 complexes may be key mediators of this process.
Title: Brca2-Pds5 complexes mobilize persistent meiotic recombination sites to the nuclear envelope.

A mutation in Cyp6d2, a cytochrome P450 gene, when combined with a brca2 mutation, resulted in synergistic hypersensitivity to camptothecin.
Title: Common variants of Drosophila melanogaster Cyp6d2 cause camptothecin sensitivity and synergize with loss of Brca2.

DNA repair by homologous recombination is dramatically decreased in CG30169 (brca2 homolog) mutants.
Title: Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpoint.

The following Brca2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brca2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11185	NM_138081.4 Latest version!	Drosophila melanogaster BRCA2, DNA repair associated (Brca2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brca2 ( NM_138081.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611925.2 Drosophila melanogaster BRCA2, DNA repair associated (Brca2), mRNA.

Price & Availability↑

CloneID	OFa11185
Clone ID Related Accession (Same CDS sequence)	NM_138081.4
Accession Version	NM_138081.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	BRCA2, DNA repair associated
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138081.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCAGA ATGGCGCATC GGGAAGTCAT CCGAACAGGC TATCCCAAGG AAGAGGAGCC 
CATGCGCGCG AACGTGGCGC CACAGTTTCC GCGGCGGCAA ATCGGAGTAA CATTATCGAC 
GAAATGGCCA AAATATGCGA AGCCGATCGC CAGACTTTCG CCATCGCTCG ACGGACTCGG 
GGTCACGAGC GGCTTGCGGT GGACAACAGC GACTTCGTCG CCGTGGAGGA TCTTATTTTG 
TCCTACGCAG AGCCCACGCC CGAGGACCAG GTCGAGATGA TCATGAGCGA CTTTTGCTCG 
TCTCCAACAT ACGCAGAGGA TGAGGATGAG CCCAGCCATG AGTCGGAGCC GTGGTTTCGA 
TTTCGCAACA AAAGGATCAG AACCTACAGC CGGAAGAGGG ATCCCAAAAG CCACAAGGCC 
GTTCAAAACG AGAAGCGTAG AGGTTCCTCA GGCCTCTCCG TGCAGAGGGA TCTCAATACT 
TCGTTCACAT CTATGGCTTG TGATTTCGAT GCTTCATCAC AGAAGATACA CGAGGTCCTT 
TTGAACCTCA GTCAATACTT TTCCGCGACC GCGACAGCTT CCGGTCCGAC TCCTGTCCCA 
TCGCAAATAG ATCTGCCAAC CGAAGCAAGG CAGGATTCTG GTGACGAGTG TTTCAACGCT 
GAATTGGAGA ACTTGAGGGA TGACCTGTTG CAGAGCGGAT TTACATTTGA GGCCTCTTTC 
TATGAAAATG AGCATGAGCA GGAAGGTCCA GGAACTATGG CAGACACTAT TTATGGCGAT 
AGCATGCAAT CGGTTCCCAC TACGCGCCTC CGATTGGAAA GTGGAAAACG TAATGTTTGG 
TCTGATGCAG ACTCTACATT GAAAAAAGCT GATGTCGAAA TTGCTGAAAA TAAGAAACTT 
TTGATGGGCC AAACGCAAGC AAAAAATGTT GACATAGAGG AGAACACAAA CTTCATATTA 
GAAGGCATAC CACTAAGTGA ATGGCTAACT CCGATGGAAC TGCCCGAGAT CTCCAAAGAT 
GTAATCAAAC ATATACCCGA TAAAAAGTTA AAGTTGGAGC CAAGTTCCCA GAAAGAACAA 
AAAAGTAGCA AAGATTCAAA TGCATCAAAG GTTAGAGGAG CCTCAAAACA ATCTTGTGAT 
ATTAACACGA AAAACGAAGG AACTACTATT CTGGATCAGC CTAATGCTGC AGAGCAAGAA 
AACCTATTGA ACGACGGCGA GCTTCTTGAG GAGTTTCTTT TTAATGATTG GCAACCAATG 
CAGTGCTCTA ATGGCCCATC AACCTCCAAA AACGCAATTC AAGGGCCTAA AGAAAACATC 
AATTCAATAA ATCTGGACGA CAAGGAGCAA CCAGAAAAGC AGACTCCTAA TAAAAGTCAG 
ACCATAAGCA GTCATCAACT TAATGGCATT CGAAAGAAGT CCTTCAAATT TATCGAGGTT 
TCGGAAGAAA TGAAAATTAA AGGCGAAGAG TTTGTAGACA AAGTTGTATC CGGCCTATAT 
CACTTTAGTC ACAAGTGCAA CTTACGTACT GAGGAATACT CCGATAATCA TAGTCAAGTA 
ATGGAATCAA CACAGTGTGC GGAATTCAAA TCAGCCCCAA GCAAGCCCAT CGAAATATTG 
GATGGTAAGG AAACCTACAA TGCAATAGCT AAAGTTGACG TTGGAGAAAT AAATGGAAAA 
TTCAGTCCTC TCAATAACGA TACTATAGCA GAGCCCGAGT TTTGTGGCTT TCGCACAGCC 
TCAAATAAGG CAATTCCGAT TTCTGAGAAG ATGAAAATCA AAACTGCAGA GTTTATGGCT 
GAGTTTCAAT CCAAAGAAAC AATTCAACAA AATGATTATT TAGTCCATCA GCCAAATGAC 
AAACCGACGT CTGTTGGACT TGATACAGCC TTAAAAAGAT CAATAGAGAG TTCAGAGGAA 
ATGCGATCGA AAGCTTCCAA GTTGGTGGTC GTTGATACTA CAATGCGGGA ACCGCATCAA 
CCCACACTTG ACCCAGTTTG CCGTGACTTA AATGAATCAC AGTTTTTCGG CTTTAGAACA 
GCGTCAAACA AAGCCATTGA GATTACCGAG GCGATGGAGA AGAGAGGAGC CATGTTTCTG 
GCCCAGTCTA AAGCTACAGA CCAATTAAAC GGGTGGCAGC CAAGCGATTT TCCTGATGTT 
CTACCCACCT CTCCAAACAA TGAGATTCAT TCAATAAATG TTGAAAATAA TAAGGCTGTG 
AATACTAAAA CCGTATCTGA GACCGAATTC TTCGGCTTTC GTACCGCTTC AAATAAAGGA 
ATTGTGATTT CGGAAAATAC GAAAATCAAA GTTGCACAGT TCATGAGTGA ATTTCAAGCC 
GCCGATGCAA GCACTGACTC GAACCAACCA ACTGTGATCT CTGAGGAATC GAGAAATATT 
GATGCCAAGT TCGTAGATGA AGCGGCGGCA GAAGACAGTG CCAACAAGCC AACTTTTTGC 
AATGTACAAA GTCTAAAAAA TACCTCAGAT ATTGAGCATT TCAAACACGA CTTCTTCGTG 
GAGCATTCTG CAAAGGAAGA GCATCCGCTG TGTAGCCAGC CATTGGTGAG GACACCCAGA 
AGGTCGCAGG AGATCCACTC CTCACTATCG CAACTTGCCG GCAAATCACC ACTGGACCAG 
GCCACCAAGA AGTCCGTAAT AGCTCGCCGG AACCTCCTGT CACTAAAAAG AAAGCGAAAG 
ATCATTTTGT CAACTGAAAC TTCCACTTCC TGCGCCTTAC CCACTATGGA GCGATTCGCT 
CCCAAGCCTT CCTCGACAAG TACTCCGCTG GCCGACAGGG ACTTAAATCG CTCCAAGGAC 
TGTACAAAGA ATAGACAGGA TGCGGAGGAC ATGTCCCCTA TTTGCATGCA ACCCAAAAAG 
AGTCGAAGAC TTGGTCTGAG TCGAAGTCGC TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611925.2
CDS	130..3045
Translation	MDQNGASGSHPNRLSQGRGAHARERGATVSAAANRSNIIDEMAKICEADRQTFAIARRTRGHERLAVDNSDFVAVEDLILSYAEPTPEDQVEMIMSDFCSSPTYAEDEDEPSHESEPWFRFRNKRIRTYSRKRDPKSHKAVQNEKRRGSSGLSVQRDLNTSFTSMACDFDASSQKIHEVLLNLSQYFSATATASGPTPVPSQIDLPTEARQDSGDECFNAELENLRDDLLQSGFTFEASFYENEHEQEGPGTMADTIYGDSMQSVPTTRLRLESGKRNVWSDADSTLKKADVEIAENKKLLMGQTQAKNVDIEENTNFILEGIPLSEWLTPMELPEISKDVIKHIPDKKLKLEPSSQKEQKSSKDSNASKVRGASKQSCDINTKNEGTTILDQPNAAEQENLLNDGELLEEFLFNDWQPMQCSNGPSTSKNAIQGPKENINSINLDDKEQPEKQTPNKSQTISSHQLNGIRKKSFKFIEVSEEMKIKGEEFVDKVVSGLYHFSHKCNLRTEEYSDNHSQVMESTQCAEFKSAPSKPIEILDGKETYNAIAKVDVGEINGKFSPLNNDTIAEPEFCGFRTASNKAIPISEKMKIKTAEFMAEFQSKETIQQNDYLVHQPNDKPTSVGLDTALKRSIESSEEMRSKASKLVVVDTTMREPHQPTLDPVCRDLNESQFFGFRTASNKAIEITEAMEKRGAMFLAQSKATDQLNGWQPSDFPDVLPTSPNNEIHSINVENNKAVNTKTVSETEFFGFRTASNKGIVISENTKIKVAQFMSEFQAADASTDSNQPTVISEESRNIDAKFVDEAAAEDSANKPTFCNVQSLKNTSDIEHFKHDFFVEHSAKEEHPLCSQPLVRTPRRSQEIHSSLSQLAGKSPLDQATKKSVIARRNLLSLKRKRKIILSTETSTSCALPTMERFAPKPSSTSTPLADRDLNRSKDCTKNRQDAEDMSPICMQPKKSRRLGLSRSRY

Target ORF information:

RefSeq Version	NM_138081.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster BRCA2, DNA repair associated (Brca2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138081.4

ORF Insert Sequence:

ATGGACCAGA ATGGCGCATC GGGAAGTCAT CCGAACAGGC TATCCCAAGG AAGAGGAGCC 
CATGCGCGCG AACGTGGCGC CACAGTTTCC GCGGCGGCAA ATCGGAGTAA CATTATCGAC 
GAAATGGCCA AAATATGCGA AGCCGATCGC CAGACTTTCG CCATCGCTCG ACGGACTCGG 
GGTCACGAGC GGCTTGCGGT GGACAACAGC GACTTCGTCG CCGTGGAGGA TCTTATTTTG 
TCCTACGCAG AGCCCACGCC CGAGGACCAG GTCGAGATGA TCATGAGCGA CTTTTGCTCG 
TCTCCAACAT ACGCAGAGGA TGAGGATGAG CCCAGCCATG AGTCGGAGCC GTGGTTTCGA 
TTTCGCAACA AAAGGATCAG AACCTACAGC CGGAAGAGGG ATCCCAAAAG CCACAAGGCC 
GTTCAAAACG AGAAGCGTAG AGGTTCCTCA GGCCTCTCCG TGCAGAGGGA TCTCAATACT 
TCGTTCACAT CTATGGCTTG TGATTTCGAT GCTTCATCAC AGAAGATACA CGAGGTCCTT 
TTGAACCTCA GTCAATACTT TTCCGCGACC GCGACAGCTT CCGGTCCGAC TCCTGTCCCA 
TCGCAAATAG ATCTGCCAAC CGAAGCAAGG CAGGATTCTG GTGACGAGTG TTTCAACGCT 
GAATTGGAGA ACTTGAGGGA TGACCTGTTG CAGAGCGGAT TTACATTTGA GGCCTCTTTC 
TATGAAAATG AGCATGAGCA GGAAGGTCCA GGAACTATGG CAGACACTAT TTATGGCGAT 
AGCATGCAAT CGGTTCCCAC TACGCGCCTC CGATTGGAAA GTGGAAAACG TAATGTTTGG 
TCTGATGCAG ACTCTACATT GAAAAAAGCT GATGTCGAAA TTGCTGAAAA TAAGAAACTT 
TTGATGGGCC AAACGCAAGC AAAAAATGTT GACATAGAGG AGAACACAAA CTTCATATTA 
GAAGGCATAC CACTAAGTGA ATGGCTAACT CCGATGGAAC TGCCCGAGAT CTCCAAAGAT 
GTAATCAAAC ATATACCCGA TAAAAAGTTA AAGTTGGAGC CAAGTTCCCA GAAAGAACAA 
AAAAGTAGCA AAGATTCAAA TGCATCAAAG GTTAGAGGAG CCTCAAAACA ATCTTGTGAT 
ATTAACACGA AAAACGAAGG AACTACTATT CTGGATCAGC CTAATGCTGC AGAGCAAGAA 
AACCTATTGA ACGACGGCGA GCTTCTTGAG GAGTTTCTTT TTAATGATTG GCAACCAATG 
CAGTGCTCTA ATGGCCCATC AACCTCCAAA AACGCAATTC AAGGGCCTAA AGAAAACATC 
AATTCAATAA ATCTGGACGA CAAGGAGCAA CCAGAAAAGC AGACTCCTAA TAAAAGTCAG 
ACCATAAGCA GTCATCAACT TAATGGCATT CGAAAGAAGT CCTTCAAATT TATCGAGGTT 
TCGGAAGAAA TGAAAATTAA AGGCGAAGAG TTTGTAGACA AAGTTGTATC CGGCCTATAT 
CACTTTAGTC ACAAGTGCAA CTTACGTACT GAGGAATACT CCGATAATCA TAGTCAAGTA 
ATGGAATCAA CACAGTGTGC GGAATTCAAA TCAGCCCCAA GCAAGCCCAT CGAAATATTG 
GATGGTAAGG AAACCTACAA TGCAATAGCT AAAGTTGACG TTGGAGAAAT AAATGGAAAA 
TTCAGTCCTC TCAATAACGA TACTATAGCA GAGCCCGAGT TTTGTGGCTT TCGCACAGCC 
TCAAATAAGG CAATTCCGAT TTCTGAGAAG ATGAAAATCA AAACTGCAGA GTTTATGGCT 
GAGTTTCAAT CCAAAGAAAC AATTCAACAA AATGATTATT TAGTCCATCA GCCAAATGAC 
AAACCGACGT CTGTTGGACT TGATACAGCC TTAAAAAGAT CAATAGAGAG TTCAGAGGAA 
ATGCGATCGA AAGCTTCCAA GTTGGTGGTC GTTGATACTA CAATGCGGGA ACCGCATCAA 
CCCACACTTG ACCCAGTTTG CCGTGACTTA AATGAATCAC AGTTTTTCGG CTTTAGAACA 
GCGTCAAACA AAGCCATTGA GATTACCGAG GCGATGGAGA AGAGAGGAGC CATGTTTCTG 
GCCCAGTCTA AAGCTACAGA CCAATTAAAC GGGTGGCAGC CAAGCGATTT TCCTGATGTT 
CTACCCACCT CTCCAAACAA TGAGATTCAT TCAATAAATG TTGAAAATAA TAAGGCTGTG 
AATACTAAAA CCGTATCTGA GACCGAATTC TTCGGCTTTC GTACCGCTTC AAATAAAGGA 
ATTGTGATTT CGGAAAATAC GAAAATCAAA GTTGCACAGT TCATGAGTGA ATTTCAAGCC 
GCCGATGCAA GCACTGACTC GAACCAACCA ACTGTGATCT CTGAGGAATC GAGAAATATT 
GATGCCAAGT TCGTAGATGA AGCGGCGGCA GAAGACAGTG CCAACAAGCC AACTTTTTGC 
AATGTACAAA GTCTAAAAAA TACCTCAGAT ATTGAGCATT TCAAACACGA CTTCTTCGTG 
GAGCATTCTG CAAAGGAAGA GCATCCGCTG TGTAGCCAGC CATTGGTGAG GACACCCAGA 
AGGTCGCAGG AGATCCACTC CTCACTATCG CAACTTGCCG GCAAATCACC ACTGGACCAG 
GCCACCAAGA AGTCCGTAAT AGCTCGCCGG AACCTCCTGT CACTAAAAAG AAAGCGAAAG 
ATCATTTTGT CAACTGAAAC TTCCACTTCC TGCGCCTTAC CCACTATGGA GCGATTCGCT 
CCCAAGCCTT CCTCGACAAG TACTCCGCTG GCCGACAGGG ACTTAAATCG CTCCAAGGAC 
TGTACAAAGA ATAGACAGGA TGCGGAGGAC ATGTCCCCTA TTTGCATGCA ACCCAAAAAG 
AGTCGAAGAC TTGGTCTGAG TCGAAGTCGC TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Brca2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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