5-HT2B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	5-HT2B
Entrez Gene ID	41017
Full Name	5-hydroxytryptamine (serotonin) receptor 2B
Synonyms	5-HT[[2B]],CG42796,CG7994,CG8007,Dmel\CG42796,Dmel_CG7994,Dmel_CG8007,anon-WO0170980.94,anon-WO0170980.95,anon-WO0170980.97,anon-WO0170980.98,d5-HT2B
General protein information	Preferred Names 5-hydroxytryptamine (serotonin) receptor 2B Names CG42796 gene product from transcript CG42796-RF 5-HT2B-PD 5-HT2B-PE 5-HT2B-PF CG42796-PD CG42796-PE CG42796-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85A3-85A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275444.2	NP_001262373.1	5-hydroxytryptamine (serotonin) receptor 2B, isoform E
NM_141549.4	NP_649806.2	5-hydroxytryptamine (serotonin) receptor 2B, isoform D
NM_001300309.1	NP_001287238.1	5-hydroxytryptamine (serotonin) receptor 2B, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG42796	NP_001262373.1
Danio rerio (zebrafish)	htr2cl2	XP_001339040.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

5-HT2b receptor is involved in the action of 5-HT on the heart rate in the larval stage.
Title: Pharmacological and genetic identification of serotonin receptor subtypes on Drosophila larval heart and aorta.

The following 5-HT2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the 5-HT2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04249	NM_001275444.2 Latest version!	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08154	NM_001300309.1 Latest version!	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08154	NM_141549.4 Latest version!	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

5-HT2B ( NM_001275444.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262373.1 Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa04249
Clone ID Related Accession (Same CDS sequence)	NM_001275444.2
Accession Version	NM_001275444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	5-hydroxytryptamine (serotonin) receptor 2B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275444.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGAGG ATGTGTATGC CTCGCTAGGT GCCTACAACG ACAGCGGTGG CGACGATTGG 
AGCAGCTCGG AGCACCTGGT CCTGTGGGAG GAGGATGAGA CGCAGCGAAC GACTGCTAAT 
GCCACCAGTC GGCATAATCA ACTGCATGTG GCCAGGTGGA ATGCCACCGG CAATGCGACC 
ATCAGCGCGA CCTTCGAGGA CGTACCCTTC GATGCGAACA ACTACTGGGC CCTATTGGCC 
CTCGTCCTCG TCCTGGGAAC AGCGGCCGGG AACATCCTTG TCTGCCTGGC CATCGCCTGG 
GAGCGCCGGC TGCAGAATGT GACCAACTAC TTCCTTATGT CGCTGGCCAT CACCGATCTC 
ATGGTCGCCG TGCTGGTCAT GCCGCTGGGC ATCCTTACGC TGGTGAAAGG CTACTTTCCT 
TTGGGCTCGG AGCACTGCCT CACCTGGATC TGCCTGGATG TACTCTTCTG CACGGCCAGC 
ATCATGCACC TGTGCACCAT CTCCGTGGAC AGATATTTAT CGCTACGATA CCCAATGCGA 
TTTGGCCGGA ATAAAACACG TCGGCGGGTC ACTCTCAAAA TTGTTTTCGT TTGGCTTCTG 
AGCATCGCCA TGAGCTTGCC CTTGAGCTTG ATGTACTCCA AGAATCACGC CTCGGTGCTG 
GTGAATGGAA CTTGCCAGAT ACCGGATCCG GTGTACAAGC TGGTCGGCTC CATTGTATGC 
TTCTACATTC CACTGGGCGT GATGCTGCTG ACATATTGCC TGACCGTCCG ACTTTTGGCC 
CGGCAGCGCC AGAATCTGGG TGGTGGACAG CAGACGGCGG CGGCCACTCC CGGATGGGCC 
AGTGGATGGC TCGGCCAGGC ACCGGCCTTG GAACGACGAT GCACCTGGCG GCGCCTTTTG 
AAACCGGGTC CAGGTAACGC CTCCTCGGTG CTGCACGCAC ACTCGGCCAA TTCAACGGAT 
ACGGATCTCA GCACTTTGGA CAACCACGAG CTCTGGCTGC CCGATTCCAG CATAAAGGAG 
CCAACGCCCA CGACCATGAC GGCCTTGCAT CAGTTCGGTG CCGAGATGCT GAAACTATCC 
CGAGGCCTGG AGTCGGTGGC CTCGTCATCC ACCACCGGCT CACCCACCAA ATCCGAATTT 
TCCCTATCGA ACCACCTGCA GCTGCAGTAC CCCAGCAGCC CACAGCGATA TGCCGCCACC 
CACCAGCAGT CCCACCACCA CAACCACCAG CAGCATCCGG CCGGAGTTTA CCACCAGCAG 
GCATCGCCCA AAGGTCGCCA CGGAACCACA GTTCTGGGTC TGTCCACCAC CACGCTGGGC 
ATCGAGCGGG AGAGCACCAG GAATTCCCTG GCCAGCAGTC GCATGGGCGG CGAACAGAGC 
GATGGGACTC TTTCACAGCT TTCCCAACGA TTGCGAGCGT ATAAAAAGCG ACGAAGAGCT 
TCGTCAGCTG TTCCTGGACG TGAGAAACGT GCCGGGCATG AGGACGCAGA CGAGGATGTG 
GAAACGCCCA CGTCAACGCT GCGCCGCCAC AAGCGCCATA ACAGTCTGCC CAAGAACGCA 
CTCTATCCGC GACACACCAC TGTACAGGAG AGCCTCGATG ACGACGACGA CGAGGAGGAG 
GAGAGCGCGC AACAGAAGGA TCAGCAGACG AAGTCGAAGA TCTGCCACTC GGACACTGAG 
CTAGATCCTC CGCAGAAGGC GAAGCAGTGC CACATAGCCG GGCAGAATCA TCAGTCGGAC 
TACCTGCAGC TGCCCTCGGT TTGCACTTGT CCGTATTTTG GGGACAGACC GCTCCAGAAT 
TGTGTGAAAT CGGCGGAGGT GAAGATTATT TCGTCCGCCT TCCGAGTGAC CACCACCACG 
ACGGCGGTGA GCAGTTCGCC CAGCGAGATG GAGCTACTCA TGTGCAGTAG TGGCAATAAA 
AAGTCATTGA CCTCCAGTGT GAGTGCCGGC ATAACAGGTG GACCGGCAGG AGCGGCTCCA 
GCCACAGTTG GCTCCACCCT CAGTCCACAC TCTGCTCACA ATCAGGGCAG CTCGCTGACA 
GTTCAGAGCG ATGGTAGTGG GTACTTGGCT GCTCCGGGAA CTCCTTGTCC TGGCCGACGA 
AAGTTGAGCA TCTCAAAGAC CGCCTCGGTG GTCACGTGGG ATTCAAGTCG TCATCGTCGG 
CGGGGCAGCA GTTTTGGCGG AGTGCGAACA TCCCTGCTGT TGACGCCCAC CAAAACAGCG 
ACCACCTCCA ACTCATCCAC ACCGTTGAGA AGATCGGCCA CCTTGAGGAG TCACCAGAAT 
ATGAACTACC AGGGAGCGGG CGAATGTGGT GGCAAGACCA GGACGACAAC ATCCTCACCC 
TGCATGTTGC AGCGTCAGCA GACGGTGCGA TCTCATCACT CGCGGAACTC CAGCGTGATC 
TCCAGAAACT CCTCGCGCCA CGGCAGGATC ATCAGGCTGG AGCAGAAGGC CACCAAGGTG 
CTGGGAGTGG TGTTCTTTAC GTTCGTAATC CTCTGGTCGC CATTCTTCGT CCTCAATCTC 
CTGCCCACGG TGTGCGCGGA GTGCGAGGAA CGAATTAGCC ACTGGGTCTT CGACGTGGTC 
ACCTGGCTGG GCTACGCTAG CTCCATGGTG AACCCCATCT TCTACACCAT CTTCAACAAG 
GTGTTCCGGC AGGCGTTCAA GAAGGTCCTG CTGTGCCGCT ATTCGAGCAC GAGTGCCTGG 
CGACCGAGCA GATAACAGAC GCCCGTTAAA CCGGGCAAAC TGAAGACGAA TACCCACAAG 
TGCGCCAGTG TGGATTTTCA GGATTCCACT GCTCCGGTGC GGGGCGTTCT GAAAAGAAGT 
GTGGCAGTGG CAAGTGACAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262373.1
CDS	644..3487
Translation	MEEDVYASLGAYNDSGGDDWSSSEHLVLWEEDETQRTTANATSRHNQLHVARWNATGNATISATFEDVPFDANNYWALLALVLVLGTAAGNILVCLAIAWERRLQNVTNYFLMSLAITDLMVAVLVMPLGILTLVKGYFPLGSEHCLTWICLDVLFCTASIMHLCTISVDRYLSLRYPMRFGRNKTRRRVTLKIVFVWLLSIAMSLPLSLMYSKNHASVLVNGTCQIPDPVYKLVGSIVCFYIPLGVMLLTYCLTVRLLARQRQNLGGGQQTAAATPGWASGWLGQAPALERRCTWRRLLKPGPGNASSVLHAHSANSTDTDLSTLDNHELWLPDSSIKEPTPTTMTALHQFGAEMLKLSRGLESVASSSTTGSPTKSEFSLSNHLQLQYPSSPQRYAATHQQSHHHNHQQHPAGVYHQQASPKGRHGTTVLGLSTTTLGIERESTRNSLASSRMGGEQSDGTLSQLSQRLRAYKKRRRASSAVPGREKRAGHEDADEDVETPTSTLRRHKRHNSLPKNALYPRHTTVQESLDDDDDEEEESAQQKDQQTKSKICHSDTELDPPQKAKQCHIAGQNHQSDYLQLPSVCTCPYFGDRPLQNCVKSAEVKIISSAFRVTTTTTAVSSSPSEMELLMCSSGNKKSLTSSVSAGITGGPAGAAPATVGSTLSPHSAHNQGSSLTVQSDGSGYLAAPGTPCPGRRKLSISKTASVVTWDSSRHRRRGSSFGGVRTSLLLTPTKTATTSNSSTPLRRSATLRSHQNMNYQGAGECGGKTRTTTSSPCMLQRQQTVRSHHSRNSSVISRNSSRHGRIIRLEQKATKVLGVVFFTFVILWSPFFVLNLLPTVCAECEERISHWVFDVVTWLGYASSMVNPIFYTIFNKVFRQAFKKVLLCRYSSTSAWRPSR*QTPVKPGKLKTNTHKCASVDFQDSTAPVRGVLKRSVAVASDI

Target ORF information:

RefSeq Version	NM_001275444.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275444.2

ORF Insert Sequence:

ATGGAAGAGG ATGTGTATGC CTCGCTAGGT GCCTACAACG ACAGCGGTGG CGACGATTGG 
AGCAGCTCGG AGCACCTGGT CCTGTGGGAG GAGGATGAGA CGCAGCGAAC GACTGCTAAT 
GCCACCAGTC GGCATAATCA ACTGCATGTG GCCAGGTGGA ATGCCACCGG CAATGCGACC 
ATCAGCGCGA CCTTCGAGGA CGTACCCTTC GATGCGAACA ACTACTGGGC CCTATTGGCC 
CTCGTCCTCG TCCTGGGAAC AGCGGCCGGG AACATCCTTG TCTGCCTGGC CATCGCCTGG 
GAGCGCCGGC TGCAGAATGT GACCAACTAC TTCCTTATGT CGCTGGCCAT CACCGATCTC 
ATGGTCGCCG TGCTGGTCAT GCCGCTGGGC ATCCTTACGC TGGTGAAAGG CTACTTTCCT 
TTGGGCTCGG AGCACTGCCT CACCTGGATC TGCCTGGATG TACTCTTCTG CACGGCCAGC 
ATCATGCACC TGTGCACCAT CTCCGTGGAC AGATATTTAT CGCTACGATA CCCAATGCGA 
TTTGGCCGGA ATAAAACACG TCGGCGGGTC ACTCTCAAAA TTGTTTTCGT TTGGCTTCTG 
AGCATCGCCA TGAGCTTGCC CTTGAGCTTG ATGTACTCCA AGAATCACGC CTCGGTGCTG 
GTGAATGGAA CTTGCCAGAT ACCGGATCCG GTGTACAAGC TGGTCGGCTC CATTGTATGC 
TTCTACATTC CACTGGGCGT GATGCTGCTG ACATATTGCC TGACCGTCCG ACTTTTGGCC 
CGGCAGCGCC AGAATCTGGG TGGTGGACAG CAGACGGCGG CGGCCACTCC CGGATGGGCC 
AGTGGATGGC TCGGCCAGGC ACCGGCCTTG GAACGACGAT GCACCTGGCG GCGCCTTTTG 
AAACCGGGTC CAGGTAACGC CTCCTCGGTG CTGCACGCAC ACTCGGCCAA TTCAACGGAT 
ACGGATCTCA GCACTTTGGA CAACCACGAG CTCTGGCTGC CCGATTCCAG CATAAAGGAG 
CCAACGCCCA CGACCATGAC GGCCTTGCAT CAGTTCGGTG CCGAGATGCT GAAACTATCC 
CGAGGCCTGG AGTCGGTGGC CTCGTCATCC ACCACCGGCT CACCCACCAA ATCCGAATTT 
TCCCTATCGA ACCACCTGCA GCTGCAGTAC CCCAGCAGCC CACAGCGATA TGCCGCCACC 
CACCAGCAGT CCCACCACCA CAACCACCAG CAGCATCCGG CCGGAGTTTA CCACCAGCAG 
GCATCGCCCA AAGGTCGCCA CGGAACCACA GTTCTGGGTC TGTCCACCAC CACGCTGGGC 
ATCGAGCGGG AGAGCACCAG GAATTCCCTG GCCAGCAGTC GCATGGGCGG CGAACAGAGC 
GATGGGACTC TTTCACAGCT TTCCCAACGA TTGCGAGCGT ATAAAAAGCG ACGAAGAGCT 
TCGTCAGCTG TTCCTGGACG TGAGAAACGT GCCGGGCATG AGGACGCAGA CGAGGATGTG 
GAAACGCCCA CGTCAACGCT GCGCCGCCAC AAGCGCCATA ACAGTCTGCC CAAGAACGCA 
CTCTATCCGC GACACACCAC TGTACAGGAG AGCCTCGATG ACGACGACGA CGAGGAGGAG 
GAGAGCGCGC AACAGAAGGA TCAGCAGACG AAGTCGAAGA TCTGCCACTC GGACACTGAG 
CTAGATCCTC CGCAGAAGGC GAAGCAGTGC CACATAGCCG GGCAGAATCA TCAGTCGGAC 
TACCTGCAGC TGCCCTCGGT TTGCACTTGT CCGTATTTTG GGGACAGACC GCTCCAGAAT 
TGTGTGAAAT CGGCGGAGGT GAAGATTATT TCGTCCGCCT TCCGAGTGAC CACCACCACG 
ACGGCGGTGA GCAGTTCGCC CAGCGAGATG GAGCTACTCA TGTGCAGTAG TGGCAATAAA 
AAGTCATTGA CCTCCAGTGT GAGTGCCGGC ATAACAGGTG GACCGGCAGG AGCGGCTCCA 
GCCACAGTTG GCTCCACCCT CAGTCCACAC TCTGCTCACA ATCAGGGCAG CTCGCTGACA 
GTTCAGAGCG ATGGTAGTGG GTACTTGGCT GCTCCGGGAA CTCCTTGTCC TGGCCGACGA 
AAGTTGAGCA TCTCAAAGAC CGCCTCGGTG GTCACGTGGG ATTCAAGTCG TCATCGTCGG 
CGGGGCAGCA GTTTTGGCGG AGTGCGAACA TCCCTGCTGT TGACGCCCAC CAAAACAGCG 
ACCACCTCCA ACTCATCCAC ACCGTTGAGA AGATCGGCCA CCTTGAGGAG TCACCAGAAT 
ATGAACTACC AGGGAGCGGG CGAATGTGGT GGCAAGACCA GGACGACAAC ATCCTCACCC 
TGCATGTTGC AGCGTCAGCA GACGGTGCGA TCTCATCACT CGCGGAACTC CAGCGTGATC 
TCCAGAAACT CCTCGCGCCA CGGCAGGATC ATCAGGCTGG AGCAGAAGGC CACCAAGGTG 
CTGGGAGTGG TGTTCTTTAC GTTCGTAATC CTCTGGTCGC CATTCTTCGT CCTCAATCTC 
CTGCCCACGG TGTGCGCGGA GTGCGAGGAA CGAATTAGCC ACTGGGTCTT CGACGTGGTC 
ACCTGGCTGG GCTACGCTAG CTCCATGGTG AACCCCATCT TCTACACCAT CTTCAACAAG 
GTGTTCCGGC AGGCGTTCAA GAAGGTCCTG CTGTGCCGCT ATTCGAGCAC GAGTGCCTGG 
CGACCGAGCA GATAACAGAC GCCCGTTAAA CCGGGCAAAC TGAAGACGAA TACCCACAAG 
TGCGCCAGTG TGGATTTTCA GGATTCCACT GCTCCGGTGC GGGGCGTTCT GAAAAGAAGT 
GTGGCAGTGG CAAGTGACAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

5-HT2B ( NM_141549.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649806.2 Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa08154
Clone ID Related Accession (Same CDS sequence)	NM_141549.4 , NM_001300309.1
Accession Version	NM_141549.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	5-hydroxytryptamine (serotonin) receptor 2B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141549.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGAGG ATGTGTATGC CTCGCTAGGT GCCTACAACG ACAGCGGTGG CGACGATTGG 
AGCAGCTCGG AGCACCTGGT CCTGTGGGAG GAGGATGAGA CGCAGCGAAC GACTGCTAAT 
GCCACCAGTC GGCATAATCA ACTGCATGTG GCCAGGTGGA ATGCCACCGG CAATGCGACC 
ATCAGCGCGA CCTTCGAGGA CGTACCCTTC GATGCGAACA ACTACTGGGC CCTATTGGCC 
CTCGTCCTCG TCCTGGGAAC AGCGGCCGGG AACATCCTTG TCTGCCTGGC CATCGCCTGG 
GAGCGCCGGC TGCAGAATGT GACCAACTAC TTCCTTATGT CGCTGGCCAT CACCGATCTC 
ATGGTCGCCG TGCTGGTCAT GCCGCTGGGC ATCCTTACGC TGGTGAAAGG CTACTTTCCT 
TTGGGCTCGG AGCACTGCCT CACCTGGATC TGCCTGGATG TACTCTTCTG CACGGCCAGC 
ATCATGCACC TGTGCACCAT CTCCGTGGAC AGATATTTAT CGCTACGATA CCCAATGCGA 
TTTGGCCGGA ATAAAACACG TCGGCGGGTC ACTCTCAAAA TTGTTTTCGT TTGGCTTCTG 
AGCATCGCCA TGAGCTTGCC CTTGAGCTTG ATGTACTCCA AGAATCACGC CTCGGTGCTG 
GTGAATGGAA CTTGCCAGAT ACCGGATCCG GTGTACAAGC TGGTCGGCTC CATTGTATGC 
TTCTACATTC CACTGGGCGT GATGCTGCTG ACATATTGCC TGACCGTCCG ACTTTTGGCC 
CGGCAGCGCC AGAATCTGGG TGGTGGACAG CAGACGGCGG CGGCCACTCC CGGATGGGCC 
AGTGGATGGC TCGGCCAGGC ACCGGCCTTG GAACGACGAT GCACCTGGCG GCGCCTTTTG 
AAACCGGGTC CAGGTAACGC CTCCTCGGTG CTGCACGCAC ACTCGGCCAA TTCAACGGAT 
ACGGATCTCA GCACTTTGGA CAACCACGAG CTCTGGCTGC CCGATTCCAG CATAAAGGAG 
CCAACGCCCA CGACCATGAC GGCCTTGCAT CAGTTCGGTG CCGAGATGCT GAAACTATCC 
CGAGGCCTGG AGTCGGTGGC CTCGTCATCC ACCACCGGCT CACCCACCAA ATCCGAATTT 
TCCCTATCGA ACCACCTGCA GCTGCAGTAC CCCAGCAGCC CACAGCGATA TGCCGCCACC 
CACCAGCAGT CCCACCACCA CAACCACCAG CAGCATCCGG CCGGAGTTTA CCACCAGCAG 
GCATCGCCCA AAGGTCGCCA CGGAACCACA GTTCTGGGTC TGTCCACCAC CACGCTGGGC 
ATCGAGCGGG AGAGCACCAG GAATTCCCTG GCCAGCAGTC GCATGGGCGG CGAACAGAGC 
GATGGGACTC TTTCACAGCT TTCCCAACGA TTGCGAGCGT ATAAAAAGCG ACGAAGAGCT 
TCGTCAGCTG TTCCTGGACG TGAGAAACGT GCCGGGCATG AGGACGCAGA CGAGGATGTG 
GAAACGCCCA CGTCAACGCT GCGCCGCCAC AAGCGCCATA ACAGTCTGCC CAAGAACGCA 
CTCTATCCGC GACACACCAC TGTACAGGAG AGCCTCGATG ACGACGACGA CGAGGAGGAG 
GAGAGCGCGC AACAGAAGGA TCAGCAGACG AAGTCGAAGA TCTGCCACTC GGACACTGAG 
CTAGATCCTC CGCAGAAGGC GAAGCAGTGC CACATAGCCG GGCAGAATCA TCAGTCGGAC 
TACCTGCAGC TGCCCTCGGT TTGCACTTGT CCGTATTTTG GGGACAGACC GCTCCAGAAT 
TGTGTGAAAT CGGCGGAGGT GAAGATTATT TCGTCCGCCT TCCGAGTGAC CACCACCACG 
ACGGCGGTGA GCAGTTCGCC CAGCGAGATG GAGCTACTCA TGTGCAGTAG TGGCAATAAA 
AAGTCATTGA CCTCCAGTGT GAGTGCCGGC ATAACAGGTG GACCGGCAGG AGCGGCTCCA 
GCCACAGTTG GCTCCACCCT CAGTCCACAC TCTGCTCACA ATCAGGGCAG CTCGCTGACA 
GTTCAGAGCG ATGGTAGTGG GTACTTGGCT GCTCCGGGAA CTCCTTGTCC TGGCCGACGA 
AAGTTGAGCA TCTCAAAGAC CGCCTCGGTG GTCACGTGGG ATTCAAGTCG TCATCGTCGG 
CGGGGCAGCA GTTTTGGCGG AGTGCGAACA TCCCTGCTGT TGACGCCCAC CAAAACAGCG 
ACCACCTCCA ACTCATCCAC ACCGTTGAGA AGATCGGCCA CCTTGAGGAG TCACCAGAAT 
ATGAACTACC AGGGAGCGGG CGAATGTGGT GGCAAGACCA GGACGACAAC ATCCTCACCC 
TGCATGTTGC AGCGTCAGCA GACGGTGCGA TCTCATCACT CGCGGAACTC CAGCGTGATC 
TCCAGAAACT CCTCGCGCCA CGGCAGGATC ATCAGGCTGG AGCAGAAGGC CACCAAGGTG 
CTGGGAGTGG TGTTCTTTAC GTTCGTAATC CTCTGGTCGC CATTCTTCGT CCTCAATCTC 
CTGCCCACGG TGTGCGCGGA GTGCGAGGAA CGAATTAGCC ACTGGGTCTT CGACGTGGTC 
ACCTGGCTGG GCTACGCTAG CTCCATGGTG AACCCCATCT TCTACACCAT CTTCAACAAG 
GTGTTCCGGC AGGCGTTCAA GAAGGTCCTG CTGTGCCGCT ATTCGAGCAC GAGTGCCTGG 
CGACCGAGCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649806.2
CDS	644..3358
Translation	MEEDVYASLGAYNDSGGDDWSSSEHLVLWEEDETQRTTANATSRHNQLHVARWNATGNATISATFEDVPFDANNYWALLALVLVLGTAAGNILVCLAIAWERRLQNVTNYFLMSLAITDLMVAVLVMPLGILTLVKGYFPLGSEHCLTWICLDVLFCTASIMHLCTISVDRYLSLRYPMRFGRNKTRRRVTLKIVFVWLLSIAMSLPLSLMYSKNHASVLVNGTCQIPDPVYKLVGSIVCFYIPLGVMLLTYCLTVRLLARQRQNLGGGQQTAAATPGWASGWLGQAPALERRCTWRRLLKPGPGNASSVLHAHSANSTDTDLSTLDNHELWLPDSSIKEPTPTTMTALHQFGAEMLKLSRGLESVASSSTTGSPTKSEFSLSNHLQLQYPSSPQRYAATHQQSHHHNHQQHPAGVYHQQASPKGRHGTTVLGLSTTTLGIERESTRNSLASSRMGGEQSDGTLSQLSQRLRAYKKRRRASSAVPGREKRAGHEDADEDVETPTSTLRRHKRHNSLPKNALYPRHTTVQESLDDDDDEEEESAQQKDQQTKSKICHSDTELDPPQKAKQCHIAGQNHQSDYLQLPSVCTCPYFGDRPLQNCVKSAEVKIISSAFRVTTTTTAVSSSPSEMELLMCSSGNKKSLTSSVSAGITGGPAGAAPATVGSTLSPHSAHNQGSSLTVQSDGSGYLAAPGTPCPGRRKLSISKTASVVTWDSSRHRRRGSSFGGVRTSLLLTPTKTATTSNSSTPLRRSATLRSHQNMNYQGAGECGGKTRTTTSSPCMLQRQQTVRSHHSRNSSVISRNSSRHGRIIRLEQKATKVLGVVFFTFVILWSPFFVLNLLPTVCAECEERISHWVFDVVTWLGYASSMVNPIFYTIFNKVFRQAFKKVLLCRYSSTSAWRPSR

Target ORF information:

RefSeq Version	NM_141549.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141549.4

ORF Insert Sequence:

ATGGAAGAGG ATGTGTATGC CTCGCTAGGT GCCTACAACG ACAGCGGTGG CGACGATTGG 
AGCAGCTCGG AGCACCTGGT CCTGTGGGAG GAGGATGAGA CGCAGCGAAC GACTGCTAAT 
GCCACCAGTC GGCATAATCA ACTGCATGTG GCCAGGTGGA ATGCCACCGG CAATGCGACC 
ATCAGCGCGA CCTTCGAGGA CGTACCCTTC GATGCGAACA ACTACTGGGC CCTATTGGCC 
CTCGTCCTCG TCCTGGGAAC AGCGGCCGGG AACATCCTTG TCTGCCTGGC CATCGCCTGG 
GAGCGCCGGC TGCAGAATGT GACCAACTAC TTCCTTATGT CGCTGGCCAT CACCGATCTC 
ATGGTCGCCG TGCTGGTCAT GCCGCTGGGC ATCCTTACGC TGGTGAAAGG CTACTTTCCT 
TTGGGCTCGG AGCACTGCCT CACCTGGATC TGCCTGGATG TACTCTTCTG CACGGCCAGC 
ATCATGCACC TGTGCACCAT CTCCGTGGAC AGATATTTAT CGCTACGATA CCCAATGCGA 
TTTGGCCGGA ATAAAACACG TCGGCGGGTC ACTCTCAAAA TTGTTTTCGT TTGGCTTCTG 
AGCATCGCCA TGAGCTTGCC CTTGAGCTTG ATGTACTCCA AGAATCACGC CTCGGTGCTG 
GTGAATGGAA CTTGCCAGAT ACCGGATCCG GTGTACAAGC TGGTCGGCTC CATTGTATGC 
TTCTACATTC CACTGGGCGT GATGCTGCTG ACATATTGCC TGACCGTCCG ACTTTTGGCC 
CGGCAGCGCC AGAATCTGGG TGGTGGACAG CAGACGGCGG CGGCCACTCC CGGATGGGCC 
AGTGGATGGC TCGGCCAGGC ACCGGCCTTG GAACGACGAT GCACCTGGCG GCGCCTTTTG 
AAACCGGGTC CAGGTAACGC CTCCTCGGTG CTGCACGCAC ACTCGGCCAA TTCAACGGAT 
ACGGATCTCA GCACTTTGGA CAACCACGAG CTCTGGCTGC CCGATTCCAG CATAAAGGAG 
CCAACGCCCA CGACCATGAC GGCCTTGCAT CAGTTCGGTG CCGAGATGCT GAAACTATCC 
CGAGGCCTGG AGTCGGTGGC CTCGTCATCC ACCACCGGCT CACCCACCAA ATCCGAATTT 
TCCCTATCGA ACCACCTGCA GCTGCAGTAC CCCAGCAGCC CACAGCGATA TGCCGCCACC 
CACCAGCAGT CCCACCACCA CAACCACCAG CAGCATCCGG CCGGAGTTTA CCACCAGCAG 
GCATCGCCCA AAGGTCGCCA CGGAACCACA GTTCTGGGTC TGTCCACCAC CACGCTGGGC 
ATCGAGCGGG AGAGCACCAG GAATTCCCTG GCCAGCAGTC GCATGGGCGG CGAACAGAGC 
GATGGGACTC TTTCACAGCT TTCCCAACGA TTGCGAGCGT ATAAAAAGCG ACGAAGAGCT 
TCGTCAGCTG TTCCTGGACG TGAGAAACGT GCCGGGCATG AGGACGCAGA CGAGGATGTG 
GAAACGCCCA CGTCAACGCT GCGCCGCCAC AAGCGCCATA ACAGTCTGCC CAAGAACGCA 
CTCTATCCGC GACACACCAC TGTACAGGAG AGCCTCGATG ACGACGACGA CGAGGAGGAG 
GAGAGCGCGC AACAGAAGGA TCAGCAGACG AAGTCGAAGA TCTGCCACTC GGACACTGAG 
CTAGATCCTC CGCAGAAGGC GAAGCAGTGC CACATAGCCG GGCAGAATCA TCAGTCGGAC 
TACCTGCAGC TGCCCTCGGT TTGCACTTGT CCGTATTTTG GGGACAGACC GCTCCAGAAT 
TGTGTGAAAT CGGCGGAGGT GAAGATTATT TCGTCCGCCT TCCGAGTGAC CACCACCACG 
ACGGCGGTGA GCAGTTCGCC CAGCGAGATG GAGCTACTCA TGTGCAGTAG TGGCAATAAA 
AAGTCATTGA CCTCCAGTGT GAGTGCCGGC ATAACAGGTG GACCGGCAGG AGCGGCTCCA 
GCCACAGTTG GCTCCACCCT CAGTCCACAC TCTGCTCACA ATCAGGGCAG CTCGCTGACA 
GTTCAGAGCG ATGGTAGTGG GTACTTGGCT GCTCCGGGAA CTCCTTGTCC TGGCCGACGA 
AAGTTGAGCA TCTCAAAGAC CGCCTCGGTG GTCACGTGGG ATTCAAGTCG TCATCGTCGG 
CGGGGCAGCA GTTTTGGCGG AGTGCGAACA TCCCTGCTGT TGACGCCCAC CAAAACAGCG 
ACCACCTCCA ACTCATCCAC ACCGTTGAGA AGATCGGCCA CCTTGAGGAG TCACCAGAAT 
ATGAACTACC AGGGAGCGGG CGAATGTGGT GGCAAGACCA GGACGACAAC ATCCTCACCC 
TGCATGTTGC AGCGTCAGCA GACGGTGCGA TCTCATCACT CGCGGAACTC CAGCGTGATC 
TCCAGAAACT CCTCGCGCCA CGGCAGGATC ATCAGGCTGG AGCAGAAGGC CACCAAGGTG 
CTGGGAGTGG TGTTCTTTAC GTTCGTAATC CTCTGGTCGC CATTCTTCGT CCTCAATCTC 
CTGCCCACGG TGTGCGCGGA GTGCGAGGAA CGAATTAGCC ACTGGGTCTT CGACGTGGTC 
ACCTGGCTGG GCTACGCTAG CTCCATGGTG AACCCCATCT TCTACACCAT CTTCAACAAG 
GTGTTCCGGC AGGCGTTCAA GAAGGTCCTG CTGTGCCGCT ATTCGAGCAC GAGTGCCTGG 
CGACCGAGCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

5-HT2B ( NM_001300309.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287238.1 Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant F, mRNA.

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CloneID	OFa08154
Clone ID Related Accession (Same CDS sequence)	NM_141549.4 , NM_001300309.1
Accession Version	NM_001300309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	5-hydroxytryptamine (serotonin) receptor 2B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGAGG ATGTGTATGC CTCGCTAGGT GCCTACAACG ACAGCGGTGG CGACGATTGG 
AGCAGCTCGG AGCACCTGGT CCTGTGGGAG GAGGATGAGA CGCAGCGAAC GACTGCTAAT 
GCCACCAGTC GGCATAATCA ACTGCATGTG GCCAGGTGGA ATGCCACCGG CAATGCGACC 
ATCAGCGCGA CCTTCGAGGA CGTACCCTTC GATGCGAACA ACTACTGGGC CCTATTGGCC 
CTCGTCCTCG TCCTGGGAAC AGCGGCCGGG AACATCCTTG TCTGCCTGGC CATCGCCTGG 
GAGCGCCGGC TGCAGAATGT GACCAACTAC TTCCTTATGT CGCTGGCCAT CACCGATCTC 
ATGGTCGCCG TGCTGGTCAT GCCGCTGGGC ATCCTTACGC TGGTGAAAGG CTACTTTCCT 
TTGGGCTCGG AGCACTGCCT CACCTGGATC TGCCTGGATG TACTCTTCTG CACGGCCAGC 
ATCATGCACC TGTGCACCAT CTCCGTGGAC AGATATTTAT CGCTACGATA CCCAATGCGA 
TTTGGCCGGA ATAAAACACG TCGGCGGGTC ACTCTCAAAA TTGTTTTCGT TTGGCTTCTG 
AGCATCGCCA TGAGCTTGCC CTTGAGCTTG ATGTACTCCA AGAATCACGC CTCGGTGCTG 
GTGAATGGAA CTTGCCAGAT ACCGGATCCG GTGTACAAGC TGGTCGGCTC CATTGTATGC 
TTCTACATTC CACTGGGCGT GATGCTGCTG ACATATTGCC TGACCGTCCG ACTTTTGGCC 
CGGCAGCGCC AGAATCTGGG TGGTGGACAG CAGACGGCGG CGGCCACTCC CGGATGGGCC 
AGTGGATGGC TCGGCCAGGC ACCGGCCTTG GAACGACGAT GCACCTGGCG GCGCCTTTTG 
AAACCGGGTC CAGGTAACGC CTCCTCGGTG CTGCACGCAC ACTCGGCCAA TTCAACGGAT 
ACGGATCTCA GCACTTTGGA CAACCACGAG CTCTGGCTGC CCGATTCCAG CATAAAGGAG 
CCAACGCCCA CGACCATGAC GGCCTTGCAT CAGTTCGGTG CCGAGATGCT GAAACTATCC 
CGAGGCCTGG AGTCGGTGGC CTCGTCATCC ACCACCGGCT CACCCACCAA ATCCGAATTT 
TCCCTATCGA ACCACCTGCA GCTGCAGTAC CCCAGCAGCC CACAGCGATA TGCCGCCACC 
CACCAGCAGT CCCACCACCA CAACCACCAG CAGCATCCGG CCGGAGTTTA CCACCAGCAG 
GCATCGCCCA AAGGTCGCCA CGGAACCACA GTTCTGGGTC TGTCCACCAC CACGCTGGGC 
ATCGAGCGGG AGAGCACCAG GAATTCCCTG GCCAGCAGTC GCATGGGCGG CGAACAGAGC 
GATGGGACTC TTTCACAGCT TTCCCAACGA TTGCGAGCGT ATAAAAAGCG ACGAAGAGCT 
TCGTCAGCTG TTCCTGGACG TGAGAAACGT GCCGGGCATG AGGACGCAGA CGAGGATGTG 
GAAACGCCCA CGTCAACGCT GCGCCGCCAC AAGCGCCATA ACAGTCTGCC CAAGAACGCA 
CTCTATCCGC GACACACCAC TGTACAGGAG AGCCTCGATG ACGACGACGA CGAGGAGGAG 
GAGAGCGCGC AACAGAAGGA TCAGCAGACG AAGTCGAAGA TCTGCCACTC GGACACTGAG 
CTAGATCCTC CGCAGAAGGC GAAGCAGTGC CACATAGCCG GGCAGAATCA TCAGTCGGAC 
TACCTGCAGC TGCCCTCGGT TTGCACTTGT CCGTATTTTG GGGACAGACC GCTCCAGAAT 
TGTGTGAAAT CGGCGGAGGT GAAGATTATT TCGTCCGCCT TCCGAGTGAC CACCACCACG 
ACGGCGGTGA GCAGTTCGCC CAGCGAGATG GAGCTACTCA TGTGCAGTAG TGGCAATAAA 
AAGTCATTGA CCTCCAGTGT GAGTGCCGGC ATAACAGGTG GACCGGCAGG AGCGGCTCCA 
GCCACAGTTG GCTCCACCCT CAGTCCACAC TCTGCTCACA ATCAGGGCAG CTCGCTGACA 
GTTCAGAGCG ATGGTAGTGG GTACTTGGCT GCTCCGGGAA CTCCTTGTCC TGGCCGACGA 
AAGTTGAGCA TCTCAAAGAC CGCCTCGGTG GTCACGTGGG ATTCAAGTCG TCATCGTCGG 
CGGGGCAGCA GTTTTGGCGG AGTGCGAACA TCCCTGCTGT TGACGCCCAC CAAAACAGCG 
ACCACCTCCA ACTCATCCAC ACCGTTGAGA AGATCGGCCA CCTTGAGGAG TCACCAGAAT 
ATGAACTACC AGGGAGCGGG CGAATGTGGT GGCAAGACCA GGACGACAAC ATCCTCACCC 
TGCATGTTGC AGCGTCAGCA GACGGTGCGA TCTCATCACT CGCGGAACTC CAGCGTGATC 
TCCAGAAACT CCTCGCGCCA CGGCAGGATC ATCAGGCTGG AGCAGAAGGC CACCAAGGTG 
CTGGGAGTGG TGTTCTTTAC GTTCGTAATC CTCTGGTCGC CATTCTTCGT CCTCAATCTC 
CTGCCCACGG TGTGCGCGGA GTGCGAGGAA CGAATTAGCC ACTGGGTCTT CGACGTGGTC 
ACCTGGCTGG GCTACGCTAG CTCCATGGTG AACCCCATCT TCTACACCAT CTTCAACAAG 
GTGTTCCGGC AGGCGTTCAA GAAGGTCCTG CTGTGCCGCT ATTCGAGCAC GAGTGCCTGG 
CGACCGAGCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287238.1
CDS	644..3358
Translation	MEEDVYASLGAYNDSGGDDWSSSEHLVLWEEDETQRTTANATSRHNQLHVARWNATGNATISATFEDVPFDANNYWALLALVLVLGTAAGNILVCLAIAWERRLQNVTNYFLMSLAITDLMVAVLVMPLGILTLVKGYFPLGSEHCLTWICLDVLFCTASIMHLCTISVDRYLSLRYPMRFGRNKTRRRVTLKIVFVWLLSIAMSLPLSLMYSKNHASVLVNGTCQIPDPVYKLVGSIVCFYIPLGVMLLTYCLTVRLLARQRQNLGGGQQTAAATPGWASGWLGQAPALERRCTWRRLLKPGPGNASSVLHAHSANSTDTDLSTLDNHELWLPDSSIKEPTPTTMTALHQFGAEMLKLSRGLESVASSSTTGSPTKSEFSLSNHLQLQYPSSPQRYAATHQQSHHHNHQQHPAGVYHQQASPKGRHGTTVLGLSTTTLGIERESTRNSLASSRMGGEQSDGTLSQLSQRLRAYKKRRRASSAVPGREKRAGHEDADEDVETPTSTLRRHKRHNSLPKNALYPRHTTVQESLDDDDDEEEESAQQKDQQTKSKICHSDTELDPPQKAKQCHIAGQNHQSDYLQLPSVCTCPYFGDRPLQNCVKSAEVKIISSAFRVTTTTTAVSSSPSEMELLMCSSGNKKSLTSSVSAGITGGPAGAAPATVGSTLSPHSAHNQGSSLTVQSDGSGYLAAPGTPCPGRRKLSISKTASVVTWDSSRHRRRGSSFGGVRTSLLLTPTKTATTSNSSTPLRRSATLRSHQNMNYQGAGECGGKTRTTTSSPCMLQRQQTVRSHHSRNSSVISRNSSRHGRIIRLEQKATKVLGVVFFTFVILWSPFFVLNLLPTVCAECEERISHWVFDVVTWLGYASSMVNPIFYTIFNKVFRQAFKKVLLCRYSSTSAWRPSR

Target ORF information:

RefSeq Version	NM_001300309.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300309.1

ORF Insert Sequence:

ATGGAAGAGG ATGTGTATGC CTCGCTAGGT GCCTACAACG ACAGCGGTGG CGACGATTGG 
AGCAGCTCGG AGCACCTGGT CCTGTGGGAG GAGGATGAGA CGCAGCGAAC GACTGCTAAT 
GCCACCAGTC GGCATAATCA ACTGCATGTG GCCAGGTGGA ATGCCACCGG CAATGCGACC 
ATCAGCGCGA CCTTCGAGGA CGTACCCTTC GATGCGAACA ACTACTGGGC CCTATTGGCC 
CTCGTCCTCG TCCTGGGAAC AGCGGCCGGG AACATCCTTG TCTGCCTGGC CATCGCCTGG 
GAGCGCCGGC TGCAGAATGT GACCAACTAC TTCCTTATGT CGCTGGCCAT CACCGATCTC 
ATGGTCGCCG TGCTGGTCAT GCCGCTGGGC ATCCTTACGC TGGTGAAAGG CTACTTTCCT 
TTGGGCTCGG AGCACTGCCT CACCTGGATC TGCCTGGATG TACTCTTCTG CACGGCCAGC 
ATCATGCACC TGTGCACCAT CTCCGTGGAC AGATATTTAT CGCTACGATA CCCAATGCGA 
TTTGGCCGGA ATAAAACACG TCGGCGGGTC ACTCTCAAAA TTGTTTTCGT TTGGCTTCTG 
AGCATCGCCA TGAGCTTGCC CTTGAGCTTG ATGTACTCCA AGAATCACGC CTCGGTGCTG 
GTGAATGGAA CTTGCCAGAT ACCGGATCCG GTGTACAAGC TGGTCGGCTC CATTGTATGC 
TTCTACATTC CACTGGGCGT GATGCTGCTG ACATATTGCC TGACCGTCCG ACTTTTGGCC 
CGGCAGCGCC AGAATCTGGG TGGTGGACAG CAGACGGCGG CGGCCACTCC CGGATGGGCC 
AGTGGATGGC TCGGCCAGGC ACCGGCCTTG GAACGACGAT GCACCTGGCG GCGCCTTTTG 
AAACCGGGTC CAGGTAACGC CTCCTCGGTG CTGCACGCAC ACTCGGCCAA TTCAACGGAT 
ACGGATCTCA GCACTTTGGA CAACCACGAG CTCTGGCTGC CCGATTCCAG CATAAAGGAG 
CCAACGCCCA CGACCATGAC GGCCTTGCAT CAGTTCGGTG CCGAGATGCT GAAACTATCC 
CGAGGCCTGG AGTCGGTGGC CTCGTCATCC ACCACCGGCT CACCCACCAA ATCCGAATTT 
TCCCTATCGA ACCACCTGCA GCTGCAGTAC CCCAGCAGCC CACAGCGATA TGCCGCCACC 
CACCAGCAGT CCCACCACCA CAACCACCAG CAGCATCCGG CCGGAGTTTA CCACCAGCAG 
GCATCGCCCA AAGGTCGCCA CGGAACCACA GTTCTGGGTC TGTCCACCAC CACGCTGGGC 
ATCGAGCGGG AGAGCACCAG GAATTCCCTG GCCAGCAGTC GCATGGGCGG CGAACAGAGC 
GATGGGACTC TTTCACAGCT TTCCCAACGA TTGCGAGCGT ATAAAAAGCG ACGAAGAGCT 
TCGTCAGCTG TTCCTGGACG TGAGAAACGT GCCGGGCATG AGGACGCAGA CGAGGATGTG 
GAAACGCCCA CGTCAACGCT GCGCCGCCAC AAGCGCCATA ACAGTCTGCC CAAGAACGCA 
CTCTATCCGC GACACACCAC TGTACAGGAG AGCCTCGATG ACGACGACGA CGAGGAGGAG 
GAGAGCGCGC AACAGAAGGA TCAGCAGACG AAGTCGAAGA TCTGCCACTC GGACACTGAG 
CTAGATCCTC CGCAGAAGGC GAAGCAGTGC CACATAGCCG GGCAGAATCA TCAGTCGGAC 
TACCTGCAGC TGCCCTCGGT TTGCACTTGT CCGTATTTTG GGGACAGACC GCTCCAGAAT 
TGTGTGAAAT CGGCGGAGGT GAAGATTATT TCGTCCGCCT TCCGAGTGAC CACCACCACG 
ACGGCGGTGA GCAGTTCGCC CAGCGAGATG GAGCTACTCA TGTGCAGTAG TGGCAATAAA 
AAGTCATTGA CCTCCAGTGT GAGTGCCGGC ATAACAGGTG GACCGGCAGG AGCGGCTCCA 
GCCACAGTTG GCTCCACCCT CAGTCCACAC TCTGCTCACA ATCAGGGCAG CTCGCTGACA 
GTTCAGAGCG ATGGTAGTGG GTACTTGGCT GCTCCGGGAA CTCCTTGTCC TGGCCGACGA 
AAGTTGAGCA TCTCAAAGAC CGCCTCGGTG GTCACGTGGG ATTCAAGTCG TCATCGTCGG 
CGGGGCAGCA GTTTTGGCGG AGTGCGAACA TCCCTGCTGT TGACGCCCAC CAAAACAGCG 
ACCACCTCCA ACTCATCCAC ACCGTTGAGA AGATCGGCCA CCTTGAGGAG TCACCAGAAT 
ATGAACTACC AGGGAGCGGG CGAATGTGGT GGCAAGACCA GGACGACAAC ATCCTCACCC 
TGCATGTTGC AGCGTCAGCA GACGGTGCGA TCTCATCACT CGCGGAACTC CAGCGTGATC 
TCCAGAAACT CCTCGCGCCA CGGCAGGATC ATCAGGCTGG AGCAGAAGGC CACCAAGGTG 
CTGGGAGTGG TGTTCTTTAC GTTCGTAATC CTCTGGTCGC CATTCTTCGT CCTCAATCTC 
CTGCCCACGG TGTGCGCGGA GTGCGAGGAA CGAATTAGCC ACTGGGTCTT CGACGTGGTC 
ACCTGGCTGG GCTACGCTAG CTCCATGGTG AACCCCATCT TCTACACCAT CTTCAACAAG 
GTGTTCCGGC AGGCGTTCAA GAAGGTCCTG CTGTGCCGCT ATTCGAGCAC GAGTGCCTGG 
CGACCGAGCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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5-HT2B cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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