KTI3 cDNA ORF clone, Glycine max(Soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	KTI3
Entrez Gene ID	547831
Full Name	Kunitz trypsin inhibitor
Synonyms	KTi,Ti-a,Ti-b,Tia,Tic,Tie
Gene Type	protein-coding
Organism	Glycine max(Soybean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001251682.2	NP_001238611.2	trypsin inhibitor A precursor
NM_001251682.1	NP_001238611.1	trypsin inhibitor A precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification of Kunitz trypsin inhibitor mutations using SNAP markers in soybean mutant lines.
Kim DS, Lee KJ, Kim JB, Kim SH, Song JY, Seo YW, Lee BM, Kang SY
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik121(4)751-60(2010 Aug)

Genetic characterization of a novel Tib-derived variant of soybean Kunitz trypsin inhibitor detected in wild soybean (Glycine soja).
Wang KJ, Yamashita T, Watanabe M, Takahata Y
Genome47(1)9-14(2004 Feb)

Structure of the Kunitz-type soybean trypsin inhibitor (STI): implication for the interactions between members of the STI family and tissue-plasminogen activator.
De Meester P, Brick P, Lloyd LF, Blow DM, Onesti S
Acta crystallographica. Section D, Biological crystallography54(Pt 4)589-97(1998 Jul)

Kunitz-type soybean trypsin inhibitor revisited: refined structure of its complex with porcine trypsin reveals an insight into the interaction between a homologous inhibitor from Erythrina caffra and tissue-type plasminogen activator.
Song HK, Suh SW
Journal of molecular biology275(2)347-63(1998 Jan)

Isolation and characterization of a 54-kilodalton precursor of caltrin, the calcium transport inhibitor protein from seminal vesicles of the rat.
Coronel CE, Novella ML, Winnica DE, Lardy HA
Biology of reproduction48(6)1326-33(1993 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that the designed single nucleotide amplified polymorphism marker was capable of identifying the KTi type and that Tia activity was higher than Tib activity in soybean.
Title: Identification of Kunitz trypsin inhibitor mutations using SNAP markers in soybean mutant lines.

The following KTI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KTI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGl59839	NM_001251682.1 Latest version!	Glycine max Kunitz trypsin inhibitor (KTI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OGl84023	NM_001251682.2 Latest version!	Glycine max Kunitz trypsin inhibitor (KTI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KTI3 ( NM_001251682.1 ) cDNA ORF clone, Glycine max(Soybean) -> NP_001238611.1 Glycine max Kunitz trypsin inhibitor (KTI3), mRNA.

Price & Availability↑

CloneID	OGl59839
Clone ID Related Accession (Same CDS sequence)	NM_001251682.1
Accession Version	NM_001251682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Glycine max(Soybean)
Product	trypsin inhibitor A precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S45092.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: S45092.1 [ECO:0000345] ##Evidence-Data-END##

ATGAAGAGCA CCATCTTCTT TCTCTTTCTC TTTTGTGCCT TCACCACCTC ATACCTACCT 
TCAGCCATCG CTGATTTCGT GCTCGATAAT GAAGGTAACC CTCTTGAAAA TGGTGGCACA 
TATTATATCT TGTCAGACAT AACAGCATTT GGTGGAATAA GAGCAGCCCC AACGGGAAAT 
GAAAGATGCC CTCTCACTGT GGTGCAATCT CGCAATGAGC TCGACAAAGG GATTGGAACA 
ATCATCTCGT CCCCATATCG AATCCGTTTT ATCGCCGAAG GCCATCCTTT GAGCCTTAAG 
TTCGATTCAT TTGCAGTTAT AATGCTGTGT GTTGGAATTC CTACCGAGTG GTCTGTTGTG 
GAGGATCTAC CAGAAGGACC TGCTGTTAAA ATTGGTGAGA ACAAAGATGC AATGGATGGT 
TGGTTTAGAC TTGAGAGAGT TTCTGATGAT GAATTCAATA ACTATAAGCT TGTGTTCTGT 
CCACAGCAAG CTGAGGATGA CAAATGTGGG GATATTGGGA TTAGTATTGA TCATGATGAT 
GGAACCAGGC GTTTGGTGGT GTCTAAGAAC AAACCGTTAG TGGTTCAGTT TCAAAAACTT 
GATAAAGAAT CACTGGCCAA GAAAAATCAT GGCCTTTCTC GCAGTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238611.1
CDS	421..1071
Translation	MKSTIFFLFLFCAFTTSYLPSAIADFVLDNEGNPLENGGTYYILSDITAFGGIRAAPTGNERCPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPTEWSVVEDLPEGPAVKIGENKDAMDGWFRLERVSDDEFNNYKLVFCPQQAEDDKCGDIGISIDHDDGTRRLVVSKNKPLVVQFQKLDKESLAKKNHGLSRSE

Target ORF information:

RefSeq Version	NM_001251682.1
Organism	Glycine max(Soybean)
Definition	Glycine max Kunitz trypsin inhibitor (KTI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251682.1

ORF Insert Sequence:

ATGAAGAGCA CCATCTTCTT TCTCTTTCTC TTTTGTGCCT TCACCACCTC ATACCTACCT 
TCAGCCATCG CTGATTTCGT GCTCGATAAT GAAGGTAACC CTCTTGAAAA TGGTGGCACA 
TATTATATCT TGTCAGACAT AACAGCATTT GGTGGAATAA GAGCAGCCCC AACGGGAAAT 
GAAAGATGCC CTCTCACTGT GGTGCAATCT CGCAATGAGC TCGACAAAGG GATTGGAACA 
ATCATCTCGT CCCCATATCG AATCCGTTTT ATCGCCGAAG GCCATCCTTT GAGCCTTAAG 
TTCGATTCAT TTGCAGTTAT AATGCTGTGT GTTGGAATTC CTACCGAGTG GTCTGTTGTG 
GAGGATCTAC CAGAAGGACC TGCTGTTAAA ATTGGTGAGA ACAAAGATGC AATGGATGGT 
TGGTTTAGAC TTGAGAGAGT TTCTGATGAT GAATTCAATA ACTATAAGCT TGTGTTCTGT 
CCACAGCAAG CTGAGGATGA CAAATGTGGG GATATTGGGA TTAGTATTGA TCATGATGAT 
GGAACCAGGC GTTTGGTGGT GTCTAAGAAC AAACCGTTAG TGGTTCAGTT TCAAAAACTT 
GATAAAGAAT CACTGGCCAA GAAAAATCAT GGCCTTTCTC GCAGTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KTI3 ( NM_001251682.2 ) cDNA ORF clone, Glycine max(Soybean) -> NP_001238611.2 Glycine max Kunitz trypsin inhibitor (KTI3), mRNA.

Price & Availability↑

CloneID	OGl84023
Clone ID Related Accession (Same CDS sequence)	NM_001251682.2
Accession Version	NM_001251682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Glycine max(soybean)
Product	trypsin inhibitor A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from ACUP03005359.1 and S45092.1. On or before Jun 2, 2017 this sequence version replaced NM_001248722.1, NM_001249823.1, NM_001251682.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGAAGAGCA CCATCTTCTT TGCTCTCTTT CTCTTTTGTG CCTTCACCAC CTCATACCTA 
CCTTCAGCCA TCGCTGATTT CGTGCTCGAT AATGAAGGTA ACCCTCTTGA AAATGGTGGC 
ACATATTATA TCTTGTCAGA CATAACAGCA TTTGGTGGAA TAAGAGCAGC CCCAACGGGA 
AATGAAAGAT GCCCTCTCAC TGTGGTGCAA TCTCGCAATG AGCTCGACAA AGGGATTGGA 
ACAATCATCT CGTCCCCATA TCGAATCCGT TTTATCGCCG AAGGCCATCC TTTGAGCCTT 
AAGTTCGATT CATTTGCAGT TATAATGCTG TGTGTTGGAA TTCCTACCGA GTGGTCTGTT 
GTGGAGGATC TACCAGAAGG ACCTGCTGTT AAAATTGGTG AGAACAAAGA TGCAATGGAT 
GGTTGGTTTA GACTTGAGAG AGTTTCTGAT GATGAATTCA ATAACTATAA GCTTGTGTTC 
TGTCCACAGC AAGCTGAGGA TGACAAATGT GGGGATATTG GGATTAGTAT TGATCATGAT 
GATGGAACCA GGCGTTTGGT GGTGTCTAAG AACAAACCGT TAGTGGTTCA GTTTCAAAAA 
CTTGATAAAG AATCACTGGC CAAGAAAAAT CATGGCCTTT CTCGCAGTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238611.2
CDS	44..697
Translation	MKSTIFFALFLFCAFTTSYLPSAIADFVLDNEGNPLENGGTYYILSDITAFGGIRAAPTGNERCPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPTEWSVVEDLPEGPAVKIGENKDAMDGWFRLERVSDDEFNNYKLVFCPQQAEDDKCGDIGISIDHDDGTRRLVVSKNKPLVVQFQKLDKESLAKKNHGLSRSE

Target ORF information:

RefSeq Version	NM_001251682.2
Organism	Glycine max(soybean)
Definition	Glycine max Kunitz trypsin inhibitor (KTI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251682.2

ORF Insert Sequence:

ATGAAGAGCA CCATCTTCTT TGCTCTCTTT CTCTTTTGTG CCTTCACCAC CTCATACCTA 
CCTTCAGCCA TCGCTGATTT CGTGCTCGAT AATGAAGGTA ACCCTCTTGA AAATGGTGGC 
ACATATTATA TCTTGTCAGA CATAACAGCA TTTGGTGGAA TAAGAGCAGC CCCAACGGGA 
AATGAAAGAT GCCCTCTCAC TGTGGTGCAA TCTCGCAATG AGCTCGACAA AGGGATTGGA 
ACAATCATCT CGTCCCCATA TCGAATCCGT TTTATCGCCG AAGGCCATCC TTTGAGCCTT 
AAGTTCGATT CATTTGCAGT TATAATGCTG TGTGTTGGAA TTCCTACCGA GTGGTCTGTT 
GTGGAGGATC TACCAGAAGG ACCTGCTGTT AAAATTGGTG AGAACAAAGA TGCAATGGAT 
GGTTGGTTTA GACTTGAGAG AGTTTCTGAT GATGAATTCA ATAACTATAA GCTTGTGTTC 
TGTCCACAGC AAGCTGAGGA TGACAAATGT GGGGATATTG GGATTAGTAT TGATCATGAT 
GATGGAACCA GGCGTTTGGT GGTGTCTAAG AACAAACCGT TAGTGGTTCA GTTTCAAAAA 
CTTGATAAAG AATCACTGGC CAAGAAAAAT CATGGCCTTT CTCGCAGTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of Kunitz trypsin inhibitor mutations using SNAP markers in soybean mutant lines.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik121(4)751-60(2010 Aug)
Kim DS,Lee KJ,Kim JB,Kim SH,Song JY,Seo YW,Lee BM,Kang SY

Genetic characterization of a novel Tib-derived variant of soybean Kunitz trypsin inhibitor detected in wild soybean (Glycine soja).
Genome47(1)9-14(2004 Feb)
Wang KJ,Yamashita T,Watanabe M,Takahata Y

Structure of the Kunitz-type soybean trypsin inhibitor (STI): implication for the interactions between members of the STI family and tissue-plasminogen activator.
Acta crystallographica. Section D, Biological crystallography54(Pt 4)589-97(1998 Jul)
De Meester P,Brick P,Lloyd LF,Blow DM,Onesti S

Kunitz-type soybean trypsin inhibitor revisited: refined structure of its complex with porcine trypsin reveals an insight into the interaction between a homologous inhibitor from Erythrina caffra and tissue-type plasminogen activator.
Journal of molecular biology275(2)347-63(1998 Jan)
Song HK,Suh SW

Isolation and characterization of a 54-kilodalton precursor of caltrin, the calcium transport inhibitor protein from seminal vesicles of the rat.
Biology of reproduction48(6)1326-33(1993 Jun)
Coronel CE,Novella ML,Winnica DE,Lardy HA