LOC112281003 gene cDNA ORF clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPn45884	XM_024517044.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45884	XM_024517045.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45884	XM_024517039.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45884	XM_024517040.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45884	XM_024517041.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45884	XM_024517042.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45884	XM_024517043.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45885	XM_024517046.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45886	XM_024517047.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45887	XM_024517048.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45888	XM_024517050.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45889	XM_024517051.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45890	XM_024517052.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn45891	XM_024517053.1 Latest version!	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

LOC112281003 ( XM_024517043.1 ) cDNA ORF clone, -> XP_024372811.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPn45884
Clone ID Related Accession (Same CDS sequence)	XM_024517043.1 , XM_024517039.1 , XM_024517040.1 , XM_024517045.1 , XM_024517041.1 , XM_024517042.1 , XM_024517044.1
Accession Version	XM_024517043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372811.1
CDS	830..6766
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517043.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517043.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517039.1 ) cDNA ORF clone, -> XP_024372807.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X1, mRNA.

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CloneID	OPn45884
Clone ID Related Accession (Same CDS sequence)	XM_024517043.1 , XM_024517039.1 , XM_024517040.1 , XM_024517045.1 , XM_024517041.1 , XM_024517042.1 , XM_024517044.1
Accession Version	XM_024517039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372807.1
CDS	974..6910
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517039.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517039.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517040.1 ) cDNA ORF clone, -> XP_024372808.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X2, mRNA.

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CloneID	OPn45884
Clone ID Related Accession (Same CDS sequence)	XM_024517043.1 , XM_024517039.1 , XM_024517040.1 , XM_024517045.1 , XM_024517041.1 , XM_024517042.1 , XM_024517044.1
Accession Version	XM_024517040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372808.1
CDS	860..6796
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517040.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517040.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517045.1 ) cDNA ORF clone, -> XP_024372813.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X7, mRNA.

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CloneID	OPn45884
Clone ID Related Accession (Same CDS sequence)	XM_024517043.1 , XM_024517039.1 , XM_024517040.1 , XM_024517045.1 , XM_024517041.1 , XM_024517042.1 , XM_024517044.1
Accession Version	XM_024517045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372813.1
CDS	839..6775
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517045.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517045.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517041.1 ) cDNA ORF clone, -> XP_024372809.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X3, mRNA.

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CloneID	OPn45884
Clone ID Related Accession (Same CDS sequence)	XM_024517043.1 , XM_024517039.1 , XM_024517040.1 , XM_024517045.1 , XM_024517041.1 , XM_024517042.1 , XM_024517044.1
Accession Version	XM_024517041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372809.1
CDS	979..6915
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517041.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517041.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517042.1 ) cDNA ORF clone, -> XP_024372810.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X4, mRNA.

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CloneID	OPn45884
Clone ID Related Accession (Same CDS sequence)	XM_024517043.1 , XM_024517039.1 , XM_024517040.1 , XM_024517045.1 , XM_024517041.1 , XM_024517042.1 , XM_024517044.1
Accession Version	XM_024517042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372810.1
CDS	845..6781
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517042.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517042.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517044.1 ) cDNA ORF clone, -> XP_024372812.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X6, mRNA.

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CloneID	OPn45884
Clone ID Related Accession (Same CDS sequence)	XM_024517043.1 , XM_024517039.1 , XM_024517040.1 , XM_024517045.1 , XM_024517041.1 , XM_024517042.1 , XM_024517044.1
Accession Version	XM_024517044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372812.1
CDS	842..6778
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517044.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517044.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGATGCGGC AGACACATGG AGGACATCCC ACCGCGTCGT CTTCTAGTTT GGTAACCTCG 
TCCGAAATGG CTGGCGCTTC AGGTACAAGT GGGATCACGA AGAGCAGCAT TCCGGGTTCT 
GGAGTCGATC ATAATCCTCC AGCTGCATCG AGGGAGGCAG GGACAGCATC CTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517046.1 ) cDNA ORF clone, -> XP_024372814.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X8, mRNA.

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CloneID	OPn45885
Clone ID Related Accession (Same CDS sequence)	XM_024517046.1
Accession Version	XM_024517046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGTCGTTT 
GTACAACACC AACAATCACT TCAGCAGCAA CAACAGCAGC AGCAGCCAGG ACAGCATCGA 
CAATTGCAAC ATCATCATCA GCATACGAGC AACTCCTCGA CCAAATTGCA AGCACAAATG 
CAGCAACACA GTCAGCCGCT ACCAGTATCC TCGCAGAGTT ATAATGCGGG ACTCACTCGC 
ATGAAAGATC CGCAACAGCA GCAACATCAG AGACAGCAGC AGCAACAGAA GCAAATATTA 
CTCAGAGGTC ATCCAGCTAT TTCACAAGCC CAGCACATGG TGGTTTCTCC CCTTCAACCG 
GGGGTACCCA GCTTCTCTTC TCAGAACTTG AACCCACAAT CCCACATACT CTCTCCAAAA 
GCACACAATA CTCTACAAAA GCATTTAATG CAGCAAGTTA GCGGTCCTTC ACAGGGGTCC 
CAAGTTTTGG ATTCGGCAAA CATGCAGTCA TCCCTAGCTT CTCCAGCTGG ATTAGTGCAG 
CAGAATAAGC CCGATGTACA GATAACTCAA GATGATTCTG GAGACAAGGA TTCACAGCCG 
AAACAGATGC AGAAGCAAGC GCAGCAGACA ACCCAGCAAG TTGTGCAGCA GCAGGATTTG 
AGGAATGGGA AGGGTGGGAT TAGAGGGACG CAGTTGATGC AGGGTGTTTC TCCTCAGACA 
GGACCAGGGC AGGCAAACAC CTTGTCTGGC AATGTGATTG ATCAATTGCA AGGTGAAGCA 
GGCGGGCAGC TACAAGCAGG ACAAAAAGTT GTAGGTCAAT CTGTAGGCCA AGCTGCTAAG 
GCAGCGAGCG GACCTCAGGT TGGAACCGTC CCAGGCGGTT CGCAGGGTGG TCAAGTTGGT 
CAAGTACATG GAGTGCAATC ACCAAGTGTA TCGTCCGTAG TGGGTTTACA GAAGGTGTCC 
CAGCAAGTCC AACAAGCTGC GCAGGGAGTA ATTAACAAGG CAGAACAGGG AAGTCTGCCG 
CAAGCAGGTG GACAATCCAG TGGTCAGCAA GCTGGTTCAG GCGCTAGTTC AAGCCAACAG 
GCGGTATCGA CCGCGTCTCC GGTCGGTCTA ACGCAACAGT GTCAACAGTT ATGGGGTCGT 
CATGTTGCGC AGGCGGCAGC GCCTCGAAGA TTGCCAATGC GACATCCTAG CAGTAGCGTG 
GGTATGCCGA TGCCGGGTCA GCTCGGCAAA ACGGTTGTAA AGCAGACGAG TGCACAGCAG 
ACGGGACAGG GTGGTCCTTC CCAAATGACA CTGCAGGCCG GACAGAATCG GTATCAGCTG 
GGTGGGACAA TTCCGATGGG CATGAGCGAC CCGAACTTTC ATTTCGTTGG CTTACCATCT 
GTTTTGAACT TGAGCACAGG TGAAGCGCAG GGCTCCCAAT GGAAAACCAA CCAGGCAATA 
GCTCATCTAC CTGGACGAAT GTACAATTTA ACACGCACTA GCAGTTCAGT GTCTGGTCAA 
TTTCCAATGT TGGCAAGTTC AACCATGCAC TCAAATGGAA ACACTGGTAT GTCGTCGCTG 
GCTGGTTCAG GTATCCAAGG TGTGGGTTTA GGGAGCATGG CGCGAGGCGT GGGTGGTGTG 
CAAGGCGACC CAGGGTTAGC GAACCTTAAA GTAGGAGCAG GTCCAGGGAT GAACGGAAGG 
GGCTTTCTAG GGTCACAGGT AGGTATTATG GGTGGTCAAA GAAACCCGGT GAATGGTTCA 
GTGGGCGATC CTGCAGGCGT AGGCAGCACA CCAAGCAGCC ATGTGAATCC TCCCGCAGGC 
TCTTCGATGC CGGCCAGCCG AACTAGTTCA AGTGCAGTTG GTGCTTCACA ATCTGGAGTT 
GTTGAATCAC GTCAAGGCGT GTCTACACAC TTTTCTAATC CGGCCGCGAG TAGCGGGCAG 
ATGCGGCAGA CACATGGAGG ACATCCCACC GCGTCGTCTT CTAGTTTGGT AACCTCGTCC 
GAAATGGCTG GCGCTTCAGG TACAAGTGGG ATCACGAAGA GCAGCATTCC GGGTTCTGGA 
GTCGATCATA ATCCTCCAGC TGCATCGAGG GAGGCAGGGA CAGCATCCTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372814.1
CDS	973..6906
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517046.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517046.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGTCGTTT 
GTACAACACC AACAATCACT TCAGCAGCAA CAACAGCAGC AGCAGCCAGG ACAGCATCGA 
CAATTGCAAC ATCATCATCA GCATACGAGC AACTCCTCGA CCAAATTGCA AGCACAAATG 
CAGCAACACA GTCAGCCGCT ACCAGTATCC TCGCAGAGTT ATAATGCGGG ACTCACTCGC 
ATGAAAGATC CGCAACAGCA GCAACATCAG AGACAGCAGC AGCAACAGAA GCAAATATTA 
CTCAGAGGTC ATCCAGCTAT TTCACAAGCC CAGCACATGG TGGTTTCTCC CCTTCAACCG 
GGGGTACCCA GCTTCTCTTC TCAGAACTTG AACCCACAAT CCCACATACT CTCTCCAAAA 
GCACACAATA CTCTACAAAA GCATTTAATG CAGCAAGTTA GCGGTCCTTC ACAGGGGTCC 
CAAGTTTTGG ATTCGGCAAA CATGCAGTCA TCCCTAGCTT CTCCAGCTGG ATTAGTGCAG 
CAGAATAAGC CCGATGTACA GATAACTCAA GATGATTCTG GAGACAAGGA TTCACAGCCG 
AAACAGATGC AGAAGCAAGC GCAGCAGACA ACCCAGCAAG TTGTGCAGCA GCAGGATTTG 
AGGAATGGGA AGGGTGGGAT TAGAGGGACG CAGTTGATGC AGGGTGTTTC TCCTCAGACA 
GGACCAGGGC AGGCAAACAC CTTGTCTGGC AATGTGATTG ATCAATTGCA AGGTGAAGCA 
GGCGGGCAGC TACAAGCAGG ACAAAAAGTT GTAGGTCAAT CTGTAGGCCA AGCTGCTAAG 
GCAGCGAGCG GACCTCAGGT TGGAACCGTC CCAGGCGGTT CGCAGGGTGG TCAAGTTGGT 
CAAGTACATG GAGTGCAATC ACCAAGTGTA TCGTCCGTAG TGGGTTTACA GAAGGTGTCC 
CAGCAAGTCC AACAAGCTGC GCAGGGAGTA ATTAACAAGG CAGAACAGGG AAGTCTGCCG 
CAAGCAGGTG GACAATCCAG TGGTCAGCAA GCTGGTTCAG GCGCTAGTTC AAGCCAACAG 
GCGGTATCGA CCGCGTCTCC GGTCGGTCTA ACGCAACAGT GTCAACAGTT ATGGGGTCGT 
CATGTTGCGC AGGCGGCAGC GCCTCGAAGA TTGCCAATGC GACATCCTAG CAGTAGCGTG 
GGTATGCCGA TGCCGGGTCA GCTCGGCAAA ACGGTTGTAA AGCAGACGAG TGCACAGCAG 
ACGGGACAGG GTGGTCCTTC CCAAATGACA CTGCAGGCCG GACAGAATCG GTATCAGCTG 
GGTGGGACAA TTCCGATGGG CATGAGCGAC CCGAACTTTC ATTTCGTTGG CTTACCATCT 
GTTTTGAACT TGAGCACAGG TGAAGCGCAG GGCTCCCAAT GGAAAACCAA CCAGGCAATA 
GCTCATCTAC CTGGACGAAT GTACAATTTA ACACGCACTA GCAGTTCAGT GTCTGGTCAA 
TTTCCAATGT TGGCAAGTTC AACCATGCAC TCAAATGGAA ACACTGGTAT GTCGTCGCTG 
GCTGGTTCAG GTATCCAAGG TGTGGGTTTA GGGAGCATGG CGCGAGGCGT GGGTGGTGTG 
CAAGGCGACC CAGGGTTAGC GAACCTTAAA GTAGGAGCAG GTCCAGGGAT GAACGGAAGG 
GGCTTTCTAG GGTCACAGGT AGGTATTATG GGTGGTCAAA GAAACCCGGT GAATGGTTCA 
GTGGGCGATC CTGCAGGCGT AGGCAGCACA CCAAGCAGCC ATGTGAATCC TCCCGCAGGC 
TCTTCGATGC CGGCCAGCCG AACTAGTTCA AGTGCAGTTG GTGCTTCACA ATCTGGAGTT 
GTTGAATCAC GTCAAGGCGT GTCTACACAC TTTTCTAATC CGGCCGCGAG TAGCGGGCAG 
ATGCGGCAGA CACATGGAGG ACATCCCACC GCGTCGTCTT CTAGTTTGGT AACCTCGTCC 
GAAATGGCTG GCGCTTCAGG TACAAGTGGG ATCACGAAGA GCAGCATTCC GGGTTCTGGA 
GTCGATCATA ATCCTCCAGC TGCATCGAGG GAGGCAGGGA CAGCATCCTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517047.1 ) cDNA ORF clone, -> XP_024372815.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X9, mRNA.

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CloneID	OPn45886
Clone ID Related Accession (Same CDS sequence)	XM_024517047.1
Accession Version	XM_024517047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGACACATG GAGGACATCC CACCGCGTCG TCTTCTAGTT TGGTAACCTC GTCCGAAATG 
GCTGGCGCTT CAGGTACAAG TGGGATCACG AAGAGCAGCA TTCCGGGTTC TGGAGTCGAT 
CATAATCCTC CAGCTGCATC GAGGGAGGCA GGGACAGCAT CCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372815.1
CDS	977..6904
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517047.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517047.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGCA 
ATAGCTCATC TACCTGGACG AATGTACAAT TTAACACGCA CTAGCAGTTC AGTGTCTGGT 
CAATTTCCAA TGTTGGCAAG TTCAACCATG CACTCAAATG GAAACACTGG TATGTCGTCG 
CTGGCTGGTT CAGGTATCCA AGGTGTGGGT TTAGGGAGCA TGGCGCGAGG CGTGGGTGGT 
GTGCAAGGCG ACCCAGGGTT AGCGAACCTT AAAGTAGGAG CAGGTCCAGG GATGAACGGA 
AGGGGCTTTC TAGGGTCACA GGTAGGTATT ATGGGTGGTC AAAGAAACCC GGTGAATGGT 
TCAGTGGGCG ATCCTGCAGG CGTAGGCAGC ACACCAAGCA GCCATGTGAA TCCTCCCGCA 
GGCTCTTCGA TGCCGGCCAG CCGAACTAGT TCAAGTGCAG TTGGTGCTTC ACAATCTGGA 
GTTGTTGAAT CACGTCAAGG CGTGTCTACA CACTTTTCTA ATCCGGCCGC GAGTAGCGGG 
CAGACACATG GAGGACATCC CACCGCGTCG TCTTCTAGTT TGGTAACCTC GTCCGAAATG 
GCTGGCGCTT CAGGTACAAG TGGGATCACG AAGAGCAGCA TTCCGGGTTC TGGAGTCGAT 
CATAATCCTC CAGCTGCATC GAGGGAGGCA GGGACAGCAT CCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517048.1 ) cDNA ORF clone, -> XP_024372816.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X10, mRNA.

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CloneID	OPn45887
Clone ID Related Accession (Same CDS sequence)	XM_024517048.1
Accession Version	XM_024517048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGTTCTT 
TCTAGTACCC TGACGGGAGT GGGAATTCCT TGGTCAGCTA GCGAAGATCA AGCTATTCTT 
GCGCTTGTGA TTGATTTAGG TCCGAATTGG GAACTCGTAA GTGACGTGTT GAGTTCGAAT 
TCGCAATTGA AGGGTATTTA CCGTAAACCT CAGCAGTGCA AAGAAAGGCA CAAAGCACTG 
ATGGAAAGAG TAGGTGTGGA AGAGTTGGAG AATTCGGAAG ATCCTACCTC AACGCAGCAG 
CAGTTCGCTA AAGGCATCGT TCGGGACTCG CGGGGTCAGT GTGCAGCAGA GGAGGATACC 
CTGAAGCACT TTCAGCAAAT TATCATAATT GTGCAAAAGT ATGGTGCTCA AAAAACTCCG 
AGTGAGTGTA AAGATCAAAA GCTCTTGCAA TCTTCACATC CTTCTCACGA AGTTGTCATC 
TCTCAGTTCT GTTACAATGT TCCTCCTGAT CCCGTTCAAT TGGCGGACTG GGTGACGAGC 
CCTGGCGACG GTTTTACTCC ACACCTAGCA CATGGTTTCC AGGGAAGTGT GGCGGGGGCT 
CAAAGTGTTG GACCAATTCC TGGACTTGGA ATGAGACCTT CAGCAGGTGG ACATCCTCTC 
CCCCCTGTGT TGCAAGCCAC AAATAGTTCG CATCTGGTAG GAGGTCCCGT GTCCCCTGCG 
TTTATGAGCA CTACTGCCTC TAGGGATGGT CAAAGGTTCA CTGTGAACTC TACAAACCCT 
CCCAACGAAG AGGATGCAAA GTTGCAAATG GCGACAAGCA AGCTAACGGA GTTCGATGCG 
CGGCGAGTAC AACAGCAGGC TGGTTCAGTT GCATCAGGAT CACCTGTCCT TAGAGGTCTG 
TCAAATTCGA ATGGACTTCT CATGTCGCCG AGCAGTACAA GTGGGGCAAT GGTGACAGAA 
TTGAACTATG GACTGTCCCT TCCAAGATCA GGTTTAGGAT GCTTGAATGC AGCTGGTTTG 
AACGGGATGC TCACTTCATC AGGGAATTTC GTTGGATCCA ACAGAACGAG GAGAATCGCA 
GGGCTGGTGA ATTTGGCACG GAGCTGCACG GAGGAACAAC GGTTGTTGGT TCTTCAAGAG 
CTTCAGTCGT TGGCTCAACA AGGCGAAACA CAAGCCGCTT CTGCTCTATC TAGCCTGATG 
GGTGATATGA CAAATGCAGT GGACGGGTCA AACCAGCAGT CGTTTGTACA ACACCAACAA 
TCACTTCAGC AGCAACAACA GCAGCAGCAG CCAGGACAGC ATCGACAATT GCAACATCAT 
CATCAGCATA CGAGCAACTC CTCGACCAAA TTGCAAGCAC AAATGCAGCA ACACAGTCAG 
CCGCTACCAG TATCCTCGCA GAGTTATAAT GCGGGACTCA CTCGCATGAA AGATCCGCAA 
CAGCAGCAAC ATCAGAGACA GCAGCAGCAA CAGAAGCAAA TATTACTCAG AGGTCATCCA 
GCTATTTCAC AAGCCCAGCA CATGGTGGTT TCTCCCCTTC AACCGGGGGT ACCCAGCTTC 
TCTTCTCAGA ACTTGAACCC ACAATCCCAC ATACTCTCTC CAAAAGCACA CAATACTCTA 
CAAAAGCATT TAATGCAGCA AGTTAGCGGT CCTTCACAGG GGTCCCAAGT TTTGGATTCG 
GCAAACATGC AGTCATCCCT AGCTTCTCCA GCTGGATTAG TGCAGCAGAA TAAGCCCGAT 
GTACAGATAA CTCAAGATGA TTCTGGAGAC AAGGATTCAC AGCCGAAACA GATGCAGAAG 
CAAGCGCAGC AGACAACCCA GCAAGTTGTG CAGCAGCAGG ATTTGAGGAA TGGGAAGGGT 
GGGATTAGAG GGACGCAGTT GATGCAGGGT GTTTCTCCTC AGACAGGACC AGGGCAGGCA 
AACACCTTGT CTGGCAATGT GATTGATCAA TTGCAAGGTG AAGCAGGCGG GCAGCTACAA 
GCAGGACAAA AAGTTGTAGG TCAATCTGTA GGCCAAGCTG CTAAGGCAGC GAGCGGACCT 
CAGGTTGGAA CCGTCCCAGG CGGTTCGCAG GGTGGTCAAG TTGGTCAAGT ACATGGAGTG 
CAATCACCAA GTGTATCGTC CGTAGTGGGT TTACAGAAGG TGTCCCAGCA AGTCCAACAA 
GCTGCGCAGG GAGTAATTAA CAAGGCAGAA CAGGGAAGTC TGCCGCAAGC AGGTGGACAA 
TCCAGTGGTC AGCAAGCTGG TTCAGGCGCT AGTTCAAGCC AACAGGCGGT ATCGACCGCG 
TCTCCGGTCG GTCTAACGCA ACAGTGTCAA CAGTTATGGG GTCGTCATGT TGCGCAGGCG 
GCAGCGCCTC GAAGATTGCC AATGCGACAT CCTAGCAGTA GCGTGGGTAT GCCGATGCCG 
GGTCAGCTCG GCAAAACGGT TGTAAAGCAG ACGAGTGCAC AGCAGACGGG ACAGGGTGGT 
CCTTCCCAAA TGACACTGCA GGCCGGACAG AATCGGTATC AGCTGGGTGG GACAATTCCG 
ATGGGCATGA GCGACCCGAA CTTTCATTTC GTTGGCTTAC CATCTGTTTT GAACTTGAGC 
ACAGGTGAAG CGCAGGGCTC CCAATGGAAA ACCAACCAGG CAATAGCTCA TCTACCTGGA 
CGAATGTACA ATTTAACACG CACTAGCAGT TCAGTGTCTG GTCAATTTCC AATGTTGGCA 
AGTTCAACCA TGCACTCAAA TGGAAACACT GGTATGTCGT CGCTGGCTGG TTCAGGTATC 
CAAGGTGTGG GTTTAGGGAG CATGGCGCGA GGCGTGGGTG GTGTGCAAGG CGACCCAGGG 
TTAGCGAACC TTAAAGTAGG AGCAGGTCCA GGGATGAACG GAAGGGGCTT TCTAGGGTCA 
CAGGTAGGTA TTATGGGTGG TCAAAGAAAC CCGGTGAATG GTTCAGTGGG CGATCCTGCA 
GGCGTAGGCA GCACACCAAG CAGCCATGTG AATCCTCCCG CAGGCTCTTC GATGCCGGCC 
AGCCGAACTA GTTCAAGTGC AGTTGGTGCT TCACAATCTG GAGTTGTTGA ATCACGTCAA 
GGCGTGTCTA CACACTTTTC TAATCCGGCC GCGAGTAGCG GGCAGATGCG GCAGACACAT 
GGAGGACATC CCACCGCGTC GTCTTCTAGT TTGGTAACCT CGTCCGAAAT GGCTGGCGCT 
TCAGGTACAA GTGGGATCAC GAAGAGCAGC ATTCCGGGTT CTGGAGTCGA TCATAATCCT 
CCAGCTGCAT CGAGGGAGGC AGGGACAGCA TCCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372816.1
CDS	974..6892
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517048.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517048.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGTTCTT 
TCTAGTACCC TGACGGGAGT GGGAATTCCT TGGTCAGCTA GCGAAGATCA AGCTATTCTT 
GCGCTTGTGA TTGATTTAGG TCCGAATTGG GAACTCGTAA GTGACGTGTT GAGTTCGAAT 
TCGCAATTGA AGGGTATTTA CCGTAAACCT CAGCAGTGCA AAGAAAGGCA CAAAGCACTG 
ATGGAAAGAG TAGGTGTGGA AGAGTTGGAG AATTCGGAAG ATCCTACCTC AACGCAGCAG 
CAGTTCGCTA AAGGCATCGT TCGGGACTCG CGGGGTCAGT GTGCAGCAGA GGAGGATACC 
CTGAAGCACT TTCAGCAAAT TATCATAATT GTGCAAAAGT ATGGTGCTCA AAAAACTCCG 
AGTGAGTGTA AAGATCAAAA GCTCTTGCAA TCTTCACATC CTTCTCACGA AGTTGTCATC 
TCTCAGTTCT GTTACAATGT TCCTCCTGAT CCCGTTCAAT TGGCGGACTG GGTGACGAGC 
CCTGGCGACG GTTTTACTCC ACACCTAGCA CATGGTTTCC AGGGAAGTGT GGCGGGGGCT 
CAAAGTGTTG GACCAATTCC TGGACTTGGA ATGAGACCTT CAGCAGGTGG ACATCCTCTC 
CCCCCTGTGT TGCAAGCCAC AAATAGTTCG CATCTGGTAG GAGGTCCCGT GTCCCCTGCG 
TTTATGAGCA CTACTGCCTC TAGGGATGGT CAAAGGTTCA CTGTGAACTC TACAAACCCT 
CCCAACGAAG AGGATGCAAA GTTGCAAATG GCGACAAGCA AGCTAACGGA GTTCGATGCG 
CGGCGAGTAC AACAGCAGGC TGGTTCAGTT GCATCAGGAT CACCTGTCCT TAGAGGTCTG 
TCAAATTCGA ATGGACTTCT CATGTCGCCG AGCAGTACAA GTGGGGCAAT GGTGACAGAA 
TTGAACTATG GACTGTCCCT TCCAAGATCA GGTTTAGGAT GCTTGAATGC AGCTGGTTTG 
AACGGGATGC TCACTTCATC AGGGAATTTC GTTGGATCCA ACAGAACGAG GAGAATCGCA 
GGGCTGGTGA ATTTGGCACG GAGCTGCACG GAGGAACAAC GGTTGTTGGT TCTTCAAGAG 
CTTCAGTCGT TGGCTCAACA AGGCGAAACA CAAGCCGCTT CTGCTCTATC TAGCCTGATG 
GGTGATATGA CAAATGCAGT GGACGGGTCA AACCAGCAGT CGTTTGTACA ACACCAACAA 
TCACTTCAGC AGCAACAACA GCAGCAGCAG CCAGGACAGC ATCGACAATT GCAACATCAT 
CATCAGCATA CGAGCAACTC CTCGACCAAA TTGCAAGCAC AAATGCAGCA ACACAGTCAG 
CCGCTACCAG TATCCTCGCA GAGTTATAAT GCGGGACTCA CTCGCATGAA AGATCCGCAA 
CAGCAGCAAC ATCAGAGACA GCAGCAGCAA CAGAAGCAAA TATTACTCAG AGGTCATCCA 
GCTATTTCAC AAGCCCAGCA CATGGTGGTT TCTCCCCTTC AACCGGGGGT ACCCAGCTTC 
TCTTCTCAGA ACTTGAACCC ACAATCCCAC ATACTCTCTC CAAAAGCACA CAATACTCTA 
CAAAAGCATT TAATGCAGCA AGTTAGCGGT CCTTCACAGG GGTCCCAAGT TTTGGATTCG 
GCAAACATGC AGTCATCCCT AGCTTCTCCA GCTGGATTAG TGCAGCAGAA TAAGCCCGAT 
GTACAGATAA CTCAAGATGA TTCTGGAGAC AAGGATTCAC AGCCGAAACA GATGCAGAAG 
CAAGCGCAGC AGACAACCCA GCAAGTTGTG CAGCAGCAGG ATTTGAGGAA TGGGAAGGGT 
GGGATTAGAG GGACGCAGTT GATGCAGGGT GTTTCTCCTC AGACAGGACC AGGGCAGGCA 
AACACCTTGT CTGGCAATGT GATTGATCAA TTGCAAGGTG AAGCAGGCGG GCAGCTACAA 
GCAGGACAAA AAGTTGTAGG TCAATCTGTA GGCCAAGCTG CTAAGGCAGC GAGCGGACCT 
CAGGTTGGAA CCGTCCCAGG CGGTTCGCAG GGTGGTCAAG TTGGTCAAGT ACATGGAGTG 
CAATCACCAA GTGTATCGTC CGTAGTGGGT TTACAGAAGG TGTCCCAGCA AGTCCAACAA 
GCTGCGCAGG GAGTAATTAA CAAGGCAGAA CAGGGAAGTC TGCCGCAAGC AGGTGGACAA 
TCCAGTGGTC AGCAAGCTGG TTCAGGCGCT AGTTCAAGCC AACAGGCGGT ATCGACCGCG 
TCTCCGGTCG GTCTAACGCA ACAGTGTCAA CAGTTATGGG GTCGTCATGT TGCGCAGGCG 
GCAGCGCCTC GAAGATTGCC AATGCGACAT CCTAGCAGTA GCGTGGGTAT GCCGATGCCG 
GGTCAGCTCG GCAAAACGGT TGTAAAGCAG ACGAGTGCAC AGCAGACGGG ACAGGGTGGT 
CCTTCCCAAA TGACACTGCA GGCCGGACAG AATCGGTATC AGCTGGGTGG GACAATTCCG 
ATGGGCATGA GCGACCCGAA CTTTCATTTC GTTGGCTTAC CATCTGTTTT GAACTTGAGC 
ACAGGTGAAG CGCAGGGCTC CCAATGGAAA ACCAACCAGG CAATAGCTCA TCTACCTGGA 
CGAATGTACA ATTTAACACG CACTAGCAGT TCAGTGTCTG GTCAATTTCC AATGTTGGCA 
AGTTCAACCA TGCACTCAAA TGGAAACACT GGTATGTCGT CGCTGGCTGG TTCAGGTATC 
CAAGGTGTGG GTTTAGGGAG CATGGCGCGA GGCGTGGGTG GTGTGCAAGG CGACCCAGGG 
TTAGCGAACC TTAAAGTAGG AGCAGGTCCA GGGATGAACG GAAGGGGCTT TCTAGGGTCA 
CAGGTAGGTA TTATGGGTGG TCAAAGAAAC CCGGTGAATG GTTCAGTGGG CGATCCTGCA 
GGCGTAGGCA GCACACCAAG CAGCCATGTG AATCCTCCCG CAGGCTCTTC GATGCCGGCC 
AGCCGAACTA GTTCAAGTGC AGTTGGTGCT TCACAATCTG GAGTTGTTGA ATCACGTCAA 
GGCGTGTCTA CACACTTTTC TAATCCGGCC GCGAGTAGCG GGCAGATGCG GCAGACACAT 
GGAGGACATC CCACCGCGTC GTCTTCTAGT TTGGTAACCT CGTCCGAAAT GGCTGGCGCT 
TCAGGTACAA GTGGGATCAC GAAGAGCAGC ATTCCGGGTT CTGGAGTCGA TCATAATCCT 
CCAGCTGCAT CGAGGGAGGC AGGGACAGCA TCCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517050.1 ) cDNA ORF clone, -> XP_024372818.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X11, mRNA.

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CloneID	OPn45888
Clone ID Related Accession (Same CDS sequence)	XM_024517050.1
Accession Version	XM_024517050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA AGTCTGCCGC AAGCAGGTGG ACAATCCAGT 
GGTCAGCAAG CTGGTTCAGG CGCTAGTTCA AGCCAACAGG CGGTATCGAC CGCGTCTCCG 
GTCGGTCTAA CGCAACAGTG TCAACAGTTA TGGGGTCGTC ATGTTGCGCA GGCGGCAGCG 
CCTCGAAGAT TGCCAATGCG ACATCCTAGC AGTAGCGTGG GTATGCCGAT GCCGGGTCAG 
CTCGGCAAAA CGGTTGTAAA GCAGACGAGT GCACAGCAGA CGGGACAGGG TGGTCCTTCC 
CAAATGACAC TGCAGGCCGG ACAGAATCGG TATCAGCTGG GTGGGACAAT TCCGATGGGC 
ATGAGCGACC CGAACTTTCA TTTCGTTGGC TTACCATCTG TTTTGAACTT GAGCACAGGT 
GAAGCGCAGG GCTCCCAATG GAAAACCAAC CAGGCAATAG CTCATCTACC TGGACGAATG 
TACAATTTAA CACGCACTAG CAGTTCAGTG TCTGGTCAAT TTCCAATGTT GGCAAGTTCA 
ACCATGCACT CAAATGGAAA CACTGGTATG TCGTCGCTGG CTGGTTCAGG TATCCAAGGT 
GTGGGTTTAG GGAGCATGGC GCGAGGCGTG GGTGGTGTGC AAGGCGACCC AGGGTTAGCG 
AACCTTAAAG TAGGAGCAGG TCCAGGGATG AACGGAAGGG GCTTTCTAGG GTCACAGGTA 
GGTATTATGG GTGGTCAAAG AAACCCGGTG AATGGTTCAG TGGGCGATCC TGCAGGCGTA 
GGCAGCACAC CAAGCAGCCA TGTGAATCCT CCCGCAGGCT CTTCGATGCC GGCCAGCCGA 
ACTAGTTCAA GTGCAGTTGG TGCTTCACAA TCTGGAGTTG TTGAATCACG TCAAGGCGTG 
TCTACACACT TTTCTAATCC GGCCGCGAGT AGCGGGCAGA TGCGGCAGAC ACATGGAGGA 
CATCCCACCG CGTCGTCTTC TAGTTTGGTA ACCTCGTCCG AAATGGCTGG CGCTTCAGGT 
ACAAGTGGGA TCACGAAGAG CAGCATTCCG GGTTCTGGAG TCGATCATAA TCCTCCAGCT 
GCATCGAGGG AGGCAGGGAC AGCATCCTCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372818.1
CDS	976..6888
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQAIAHLPGRMYNLTRTSSSVSGQFPMLASSTMHSNGNTGMSSLAGSGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517050.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517050.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA AGTCTGCCGC AAGCAGGTGG ACAATCCAGT 
GGTCAGCAAG CTGGTTCAGG CGCTAGTTCA AGCCAACAGG CGGTATCGAC CGCGTCTCCG 
GTCGGTCTAA CGCAACAGTG TCAACAGTTA TGGGGTCGTC ATGTTGCGCA GGCGGCAGCG 
CCTCGAAGAT TGCCAATGCG ACATCCTAGC AGTAGCGTGG GTATGCCGAT GCCGGGTCAG 
CTCGGCAAAA CGGTTGTAAA GCAGACGAGT GCACAGCAGA CGGGACAGGG TGGTCCTTCC 
CAAATGACAC TGCAGGCCGG ACAGAATCGG TATCAGCTGG GTGGGACAAT TCCGATGGGC 
ATGAGCGACC CGAACTTTCA TTTCGTTGGC TTACCATCTG TTTTGAACTT GAGCACAGGT 
GAAGCGCAGG GCTCCCAATG GAAAACCAAC CAGGCAATAG CTCATCTACC TGGACGAATG 
TACAATTTAA CACGCACTAG CAGTTCAGTG TCTGGTCAAT TTCCAATGTT GGCAAGTTCA 
ACCATGCACT CAAATGGAAA CACTGGTATG TCGTCGCTGG CTGGTTCAGG TATCCAAGGT 
GTGGGTTTAG GGAGCATGGC GCGAGGCGTG GGTGGTGTGC AAGGCGACCC AGGGTTAGCG 
AACCTTAAAG TAGGAGCAGG TCCAGGGATG AACGGAAGGG GCTTTCTAGG GTCACAGGTA 
GGTATTATGG GTGGTCAAAG AAACCCGGTG AATGGTTCAG TGGGCGATCC TGCAGGCGTA 
GGCAGCACAC CAAGCAGCCA TGTGAATCCT CCCGCAGGCT CTTCGATGCC GGCCAGCCGA 
ACTAGTTCAA GTGCAGTTGG TGCTTCACAA TCTGGAGTTG TTGAATCACG TCAAGGCGTG 
TCTACACACT TTTCTAATCC GGCCGCGAGT AGCGGGCAGA TGCGGCAGAC ACATGGAGGA 
CATCCCACCG CGTCGTCTTC TAGTTTGGTA ACCTCGTCCG AAATGGCTGG CGCTTCAGGT 
ACAAGTGGGA TCACGAAGAG CAGCATTCCG GGTTCTGGAG TCGATCATAA TCCTCCAGCT 
GCATCGAGGG AGGCAGGGAC AGCATCCTCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517051.1 ) cDNA ORF clone, -> XP_024372819.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X12, mRNA.

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CloneID	OPn45889
Clone ID Related Accession (Same CDS sequence)	XM_024517051.1
Accession Version	XM_024517051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTATCCAA GGTGTGGGTT TAGGGAGCAT GGCGCGAGGC 
GTGGGTGGTG TGCAAGGCGA CCCAGGGTTA GCGAACCTTA AAGTAGGAGC AGGTCCAGGG 
ATGAACGGAA GGGGCTTTCT AGGGTCACAG GTAGGTATTA TGGGTGGTCA AAGAAACCCG 
GTGAATGGTT CAGTGGGCGA TCCTGCAGGC GTAGGCAGCA CACCAAGCAG CCATGTGAAT 
CCTCCCGCAG GCTCTTCGAT GCCGGCCAGC CGAACTAGTT CAAGTGCAGT TGGTGCTTCA 
CAATCTGGAG TTGTTGAATC ACGTCAAGGC GTGTCTACAC ACTTTTCTAA TCCGGCCGCG 
AGTAGCGGGC AGATGCGGCA GACACATGGA GGACATCCCA CCGCGTCGTC TTCTAGTTTG 
GTAACCTCGT CCGAAATGGC TGGCGCTTCA GGTACAAGTG GGATCACGAA GAGCAGCATT 
CCGGGTTCTG GAGTCGATCA TAATCCTCCA GCTGCATCGA GGGAGGCAGG GACAGCATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372819.1
CDS	974..6739
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGIQGVGLGSMARGVGGVQGDPGLANLKVGAGPGMNGRGFLGSQVGIMGGQRNPVNGSVGDPAGVGSTPSSHVNPPAGSSMPASRTSSSAVGASQSGVVESRQGVSTHFSNPAASSGQMRQTHGGHPTASSSSLVTSSEMAGASGTSGITKSSIPGSGVDHNPPAASREAGTASS

Target ORF information:

RefSeq Version	XM_024517051.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517051.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTATCCAA GGTGTGGGTT TAGGGAGCAT GGCGCGAGGC 
GTGGGTGGTG TGCAAGGCGA CCCAGGGTTA GCGAACCTTA AAGTAGGAGC AGGTCCAGGG 
ATGAACGGAA GGGGCTTTCT AGGGTCACAG GTAGGTATTA TGGGTGGTCA AAGAAACCCG 
GTGAATGGTT CAGTGGGCGA TCCTGCAGGC GTAGGCAGCA CACCAAGCAG CCATGTGAAT 
CCTCCCGCAG GCTCTTCGAT GCCGGCCAGC CGAACTAGTT CAAGTGCAGT TGGTGCTTCA 
CAATCTGGAG TTGTTGAATC ACGTCAAGGC GTGTCTACAC ACTTTTCTAA TCCGGCCGCG 
AGTAGCGGGC AGATGCGGCA GACACATGGA GGACATCCCA CCGCGTCGTC TTCTAGTTTG 
GTAACCTCGT CCGAAATGGC TGGCGCTTCA GGTACAAGTG GGATCACGAA GAGCAGCATT 
CCGGGTTCTG GAGTCGATCA TAATCCTCCA GCTGCATCGA GGGAGGCAGG GACAGCATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517052.1 ) cDNA ORF clone, -> XP_024372820.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X13, mRNA.

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CloneID	OPn45890
Clone ID Related Accession (Same CDS sequence)	XM_024517052.1
Accession Version	XM_024517052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGTA 
TCCAAGGTGT GGGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372820.1
CDS	979..6276
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQGVINKAEQGSLPQAGGQSSGQQAGSGASSSQQAVSTASPVGLTQQCQQLWGRHVAQAAAPRRLPMRHPSSSVGMPMPGQLGKTVVKQTSAQQTGQGGPSQMTLQAGQNRYQLGGTIPMGMSDPNFHFVGLPSVLNLSTGEAQGSQWKTNQVSKVWV

Target ORF information:

RefSeq Version	XM_024517052.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517052.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGGGA GTAATTAACA AGGCAGAACA GGGAAGTCTG 
CCGCAAGCAG GTGGACAATC CAGTGGTCAG CAAGCTGGTT CAGGCGCTAG TTCAAGCCAA 
CAGGCGGTAT CGACCGCGTC TCCGGTCGGT CTAACGCAAC AGTGTCAACA GTTATGGGGT 
CGTCATGTTG CGCAGGCGGC AGCGCCTCGA AGATTGCCAA TGCGACATCC TAGCAGTAGC 
GTGGGTATGC CGATGCCGGG TCAGCTCGGC AAAACGGTTG TAAAGCAGAC GAGTGCACAG 
CAGACGGGAC AGGGTGGTCC TTCCCAAATG ACACTGCAGG CCGGACAGAA TCGGTATCAG 
CTGGGTGGGA CAATTCCGAT GGGCATGAGC GACCCGAACT TTCATTTCGT TGGCTTACCA 
TCTGTTTTGA ACTTGAGCAC AGGTGAAGCG CAGGGCTCCC AATGGAAAAC CAACCAGGTA 
TCCAAGGTGT GGGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281003 ( XM_024517053.1 ) cDNA ORF clone, -> XP_024372821.1 Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X14, mRNA.

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CloneID	OPn45891
Clone ID Related Accession (Same CDS sequence)	XM_024517053.1
Accession Version	XM_024517053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	chromatin modification-related protein EAF1 B-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGAAA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024372821.1
CDS	986..5821
Translation	MVNMSYQVLSHQNTHALSSKWNGLASSQQYEPVGEIIARCGPVLEQHASELSSRDKASAPSVQAIQSFDVRVDSRVGVTERISDISSLTFKDRSNDLADRQETHGLTVASWERSIRDAGANRVGGSAAEDVHTNASEIVEGNVGTVEQATERRDRVGSMLCLPRVDVGRVSDSAASLRGSSNDIMPENHRSEAQSVQGMLESCIQSAVDGLSGALAHKDGRIVKKAAFSRGGNKLKMAHRGRVGVDAKKGNNHHLSLNSEEGVSEDRTNHLATEAESRGQGNIGAHPQALRTGVQAGAPRIIELSTSISGALNCMYPGVVLPWEKGQVSAIQSEADRRNADLTNKLANKAREDFILEKAEHLKGKRKLIEEGRTLKKIAELSRRKSHWDFVLEEMRWMANDFWQERMWKRYQAAHVCLEIAMKKGEDEFLAARLLKEQRRISNVLAHAVKDFWHSAEVEATKEITGTRISTKEQSKRRSNETEVIPMEVDSFVNEEESLKSVHIEKAGNEKPSGLQKYAIQLLKESGFAWIGQAEVPSTPKRYRESISILEQVHEVPLFYKVPVGVMEAYRIAVEAEYARSEKEYEKRQTVSITEAGALAGAEAPEEAGARGGVSYRNFLNDGGSQEDEPTAFTIEGSRIAKKKRRKVFKTGSVDAKSNGSGLQGLSYRLSGVRSSELGIPSPLVAEKLSICGDLGPNSLTGSIPVKRARSSAANSRPRGTHCGTSPGAVGLPAQKGFGLSSQQDDDLPMDDSSEGDINSDSPSSEVVGGDSKQRKKKKTKSYQGLSFYDVGKSVKDIVGIAYDLRPEQGKRNAETPWVTIGESDGVLNALVASGSPSISGQKNSKKQKSVRQAEENLDTTDTSGLHSEQQVASGVSVNYLNRHTSMRDGHRRVRPKKALHVLQVLSSTLTGVGIPWSASEDQAILALVIDLGPNWELVSDVLSSNSQLKGIYRKPQQCKERHKALMERVGVEELENSEDPTSTQQQFAKGIVRDSRGQCAAEEDTLKHFQQIIIIVQKYGAQKTPSECKDQKLLQSSHPSHEVVISQFCYNVPPDPVQLADWVTSPGDGFTPHLAHGFQGSVAGAQSVGPIPGLGMRPSAGGHPLPPVLQATNSSHLVGGPVSPAFMSTTASRDGQRFTVNSTNPPNEEDAKLQMATSKLTEFDARRVQQQAGSVASGSPVLRGLSNSNGLLMSPSSTSGAMVTELNYGLSLPRSGLGCLNAAGLNGMLTSSGNFVGSNRTRRIAGLVNLARSCTEEQRLLVLQELQSLAQQGETQAASALSSLMGDMTNAVDGSNQQSFVQHQQSLQQQQQQQQPGQHRQLQHHHQHTSNSSTKLQAQMQQHSQPLPVSSQSYNAGLTRMKDPQQQQHQRQQQQQKQILLRGHPAISQAQHMVVSPLQPGVPSFSSQNLNPQSHILSPKAHNTLQKHLMQQVSGPSQGSQVLDSANMQSSLASPAGLVQQNKPDVQITQDDSGDKDSQPKQMQKQAQQTTQQVVQQQDLRNGKGGIRGTQLMQGVSPQTGPGQANTLSGNVIDQLQGEAGGQLQAGQKVVGQSVGQAAKAASGPQVGTVPGGSQGGQVGQVHGVQSPSVSSVVGLQKVSQQVQQAAQKC

Target ORF information:

RefSeq Version	XM_024517053.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens chromatin modification-related protein EAF1 B-like (LOC112281003), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517053.1

ORF Insert Sequence:

ATGGTGAACA TGAGTTACCA AGTTTTGTCA CATCAGAACA CACATGCTCT GTCAAGCAAG 
TGGAATGGAT TAGCTAGTAG CCAGCAGTAT GAGCCCGTGG GCGAAATCAT AGCAAGATGT 
GGGCCGGTCT TGGAGCAGCA CGCAAGTGAG TTGAGCTCCA GGGACAAAGC TTCAGCACCT 
TCTGTTCAGG CCATACAGTC CTTCGATGTT CGAGTTGATT CCCGTGTTGG CGTTACAGAG 
AGAATTTCCG ATATTTCATC TCTGACTTTC AAAGACCGTT CGAATGATCT AGCGGACAGA 
CAAGAGACTC ATGGGCTGAC AGTCGCCTCT TGGGAGAGGT CCATCCGTGA TGCAGGTGCT 
AATCGGGTAG GGGGTTCAGC TGCTGAAGAC GTGCACACAA ATGCCTCTGA GATCGTTGAA 
GGTAATGTAG GAACTGTCGA GCAGGCGACG GAGAGACGAG ACAGGGTGGG TTCTATGTTG 
TGCCTCCCTA GGGTCGATGT TGGTAGGGTG TCCGATTCAG CAGCAAGCTT GCGGGGATCT 
TCGAATGATA TTATGCCAGA GAACCACAGA TCCGAGGCAC AGAGCGTGCA GGGAATGCTG 
GAGTCTTGCA TTCAAAGTGC TGTGGATGGT TTGAGTGGCG CGTTAGCGCA CAAAGATGGG 
CGGATAGTGA AGAAGGCCGC CTTTTCAAGG GGAGGCAATA AATTGAAAAT GGCGCACAGA 
GGAAGGGTAG GAGTGGACGC GAAGAAGGGC AACAATCATC ACTTGAGTTT GAACTCGGAG 
GAAGGCGTGT CGGAAGACAG GACCAACCAC CTCGCAACAG AGGCGGAGTC AAGGGGTCAG 
GGTAACATTG GTGCTCATCC TCAGGCACTT AGAACGGGGG TCCAAGCTGG TGCTCCTAGG 
ATCATCGAAC TGTCGACAAG TATTTCTGGT GCATTAAATT GTATGTATCC AGGGGTGGTT 
CTGCCATGGG AGAAGGGACA AGTTTCAGCA ATACAATCTG AAGCTGACCG ACGTAACGCC 
GATCTTACGA ATAAACTGGC TAATAAGGCT CGCGAAGACT TCATTCTCGA AAAGGCTGAG 
CATCTGAAGG GGAAAAGGAA GCTAATCGAG GAGGGGAGGA CTTTGAAGAA GATTGCCGAA 
CTTTCGCGGC GAAAGTCGCA TTGGGACTTT GTGTTGGAGG AAATGAGATG GATGGCCAAC 
GACTTCTGGC AGGAGAGGAT GTGGAAGAGG TATCAGGCTG CCCATGTTTG TCTGGAAATT 
GCTATGAAGA AGGGGGAAGA CGAGTTTTTG GCAGCACGTT TGCTCAAGGA GCAACGCAGA 
ATCTCGAACG TTTTAGCTCA TGCCGTGAAG GATTTTTGGC ACAGTGCAGA AGTTGAAGCT 
ACCAAGGAAA TAACCGGAAC TAGAATTTCC ACAAAAGAGC AATCCAAGAG AAGGAGTAAT 
GAGACTGAAG TTATTCCAAT GGAAGTGGAT TCTTTTGTTA ATGAGGAGGA ATCTTTGAAA 
TCGGTGCATA TTGAGAAAGC TGGAAACGAG AAGCCAAGTG GGTTACAGAA GTATGCTATT 
CAGCTTTTGA AAGAATCAGG ATTCGCCTGG ATAGGGCAAG CCGAGGTCCC TTCGACTCCC 
AAGCGTTATC GAGAAAGCAT CAGCATTCTG GAGCAAGTTC ATGAGGTACC ATTGTTTTAC 
AAGGTACCGG TTGGAGTAAT GGAAGCTTAT CGAATAGCAG TGGAAGCTGA ATACGCTAGG 
TCCGAGAAGG AGTATGAAAA GAGGCAGACA GTTTCAATCA CCGAGGCGGG GGCTCTGGCT 
GGTGCGGAAG CACCTGAGGA AGCAGGCGCG AGAGGGGGTG TGAGCTATAG AAATTTCTTG 
AATGATGGAG GTTCTCAGGA AGACGAACCT ACTGCGTTTA CTATTGAAGG ATCTCGTATA 
GCGAAGAAGA AACGGAGGAA GGTATTTAAA ACGGGCAGTG TTGATGCAAA AAGCAATGGC 
AGTGGGCTAC AAGGACTTTC ATATCGATTG TCAGGTGTTC GGAGTTCAGA GTTGGGCATA 
CCCTCACCTC TTGTAGCGGA AAAACTTTCC ATTTGTGGAG ATTTGGGACC GAACAGTTTA 
ACTGGAAGCA TCCCAGTGAA GCGCGCGCGG TCATCAGCAG CGAATTCACG ACCACGTGGC 
ACACATTGTG GCACATCTCC AGGTGCAGTT GGACTTCCGG CACAGAAGGG GTTTGGGTTG 
TCATCTCAGC AAGACGATGA CTTGCCCATG GATGATTCTA GTGAAGGTGA TATCAATAGC 
GACTCACCCA GTAGCGAAGT GGTTGGAGGT GACAGTAAGC AGAGGAAGAA AAAGAAGACC 
AAGTCATATC AAGGCCTGTC GTTTTACGAT GTAGGCAAGT CGGTAAAGGA TATAGTTGGA 
ATTGCCTATG ATCTTCGACC CGAGCAGGGG AAACGGAATG CAGAGACGCC TTGGGTAACT 
ATTGGTGAAT CCGATGGAGT TCTCAATGCA CTAGTCGCGT CAGGGTCTCC AAGCATATCA 
GGTCAGAAAA ACTCGAAGAA ACAGAAGTCT GTGAGGCAGG CGGAAGAAAA CCTCGATACA 
ACCGACACAT CTGGTTTGCA CAGCGAGCAA CAAGTTGCCT CTGGAGTGAG TGTAAATTAT 
TTAAATAGAC ACACCAGCAT GCGAGACGGT CATCGGAGGG TCCGACCGAA AAAGGCACTG 
CATGTTCTGC AGGTTCTTTC TAGTACCCTG ACGGGAGTGG GAATTCCTTG GTCAGCTAGC 
GAAGATCAAG CTATTCTTGC GCTTGTGATT GATTTAGGTC CGAATTGGGA ACTCGTAAGT 
GACGTGTTGA GTTCGAATTC GCAATTGAAG GGTATTTACC GTAAACCTCA GCAGTGCAAA 
GAAAGGCACA AAGCACTGAT GGAAAGAGTA GGTGTGGAAG AGTTGGAGAA TTCGGAAGAT 
CCTACCTCAA CGCAGCAGCA GTTCGCTAAA GGCATCGTTC GGGACTCGCG GGGTCAGTGT 
GCAGCAGAGG AGGATACCCT GAAGCACTTT CAGCAAATTA TCATAATTGT GCAAAAGTAT 
GGTGCTCAAA AAACTCCGAG TGAGTGTAAA GATCAAAAGC TCTTGCAATC TTCACATCCT 
TCTCACGAAG TTGTCATCTC TCAGTTCTGT TACAATGTTC CTCCTGATCC CGTTCAATTG 
GCGGACTGGG TGACGAGCCC TGGCGACGGT TTTACTCCAC ACCTAGCACA TGGTTTCCAG 
GGAAGTGTGG CGGGGGCTCA AAGTGTTGGA CCAATTCCTG GACTTGGAAT GAGACCTTCA 
GCAGGTGGAC ATCCTCTCCC CCCTGTGTTG CAAGCCACAA ATAGTTCGCA TCTGGTAGGA 
GGTCCCGTGT CCCCTGCGTT TATGAGCACT ACTGCCTCTA GGGATGGTCA AAGGTTCACT 
GTGAACTCTA CAAACCCTCC CAACGAAGAG GATGCAAAGT TGCAAATGGC GACAAGCAAG 
CTAACGGAGT TCGATGCGCG GCGAGTACAA CAGCAGGCTG GTTCAGTTGC ATCAGGATCA 
CCTGTCCTTA GAGGTCTGTC AAATTCGAAT GGACTTCTCA TGTCGCCGAG CAGTACAAGT 
GGGGCAATGG TGACAGAATT GAACTATGGA CTGTCCCTTC CAAGATCAGG TTTAGGATGC 
TTGAATGCAG CTGGTTTGAA CGGGATGCTC ACTTCATCAG GGAATTTCGT TGGATCCAAC 
AGAACGAGGA GAATCGCAGG GCTGGTGAAT TTGGCACGGA GCTGCACGGA GGAACAACGG 
TTGTTGGTTC TTCAAGAGCT TCAGTCGTTG GCTCAACAAG GCGAAACACA AGCCGCTTCT 
GCTCTATCTA GCCTGATGGG TGATATGACA AATGCAGTGG ACGGGTCAAA CCAGCAGTCG 
TTTGTACAAC ACCAACAATC ACTTCAGCAG CAACAACAGC AGCAGCAGCC AGGACAGCAT 
CGACAATTGC AACATCATCA TCAGCATACG AGCAACTCCT CGACCAAATT GCAAGCACAA 
ATGCAGCAAC ACAGTCAGCC GCTACCAGTA TCCTCGCAGA GTTATAATGC GGGACTCACT 
CGCATGAAAG ATCCGCAACA GCAGCAACAT CAGAGACAGC AGCAGCAACA GAAGCAAATA 
TTACTCAGAG GTCATCCAGC TATTTCACAA GCCCAGCACA TGGTGGTTTC TCCCCTTCAA 
CCGGGGGTAC CCAGCTTCTC TTCTCAGAAC TTGAACCCAC AATCCCACAT ACTCTCTCCA 
AAAGCACACA ATACTCTACA AAAGCATTTA ATGCAGCAAG TTAGCGGTCC TTCACAGGGG 
TCCCAAGTTT TGGATTCGGC AAACATGCAG TCATCCCTAG CTTCTCCAGC TGGATTAGTG 
CAGCAGAATA AGCCCGATGT ACAGATAACT CAAGATGATT CTGGAGACAA GGATTCACAG 
CCGAAACAGA TGCAGAAGCA AGCGCAGCAG ACAACCCAGC AAGTTGTGCA GCAGCAGGAT 
TTGAGGAATG GGAAGGGTGG GATTAGAGGG ACGCAGTTGA TGCAGGGTGT TTCTCCTCAG 
ACAGGACCAG GGCAGGCAAA CACCTTGTCT GGCAATGTGA TTGATCAATT GCAAGGTGAA 
GCAGGCGGGC AGCTACAAGC AGGACAAAAA GTTGTAGGTC AATCTGTAGG CCAAGCTGCT 
AAGGCAGCGA GCGGACCTCA GGTTGGAACC GTCCCAGGCG GTTCGCAGGG TGGTCAAGTT 
GGTCAAGTAC ATGGAGTGCA ATCACCAAGT GTATCGTCCG TAGTGGGTTT ACAGAAGGTG 
TCCCAGCAAG TCCAACAAGC TGCGCAGAAA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC112281003
Entrez Gene ID	112281003

LOC112281003 cDNA ORF clone,

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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