PRKAR2A cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKAR2A
Entrez Gene ID	397493
Full Name	protein kinase cAMP-dependent type II regulatory subunit alpha
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214258.2	NP_999423.2	cAMP-dependent protein kinase type II-alpha regulatory subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cytoplasmic anchoring of cAMP-dependent protein kinase (PKA) by A-kinase anchor proteins (AKAPs) is required for meiotic arrest of porcine full-grown and growing oocytes.
Nishimura T, Fujii W, Sugiura K, Naito K
Biology of reproduction90(3)58(2014 Mar)

A-kinase anchor protein 1 (AKAP1) regulates cAMP-dependent protein kinase (PKA) localization and is involved in meiotic maturation of porcine oocytes.
Nishimura T, Sugiura K, Naito K
Biology of reproduction88(4)85(2013 Apr)

Analyses of the involvement of PKA regulation mechanism in meiotic incompetence of porcine growing oocytes.
Nishimura T, Fujii W, Kano K, Sugiura K, Naito K
Biology of reproduction87(3)53(2012 Sep)

Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP-dependent protein kinase.
Hemmings BA, Schwarz M, Adavani SR, Jans DA
FEBS letters209(2)219-22(1986 Dec)

Correlation of the cAMP binding domain with a site of autophosphorylation on the regulatory subunit of cAMP-dependent protein kinase II from porcine skeletal muscle.
Potter RL, Taylor SS
The Journal of biological chemistry254(18)9000-5(1979 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analyses of the involvement of PKA regulation mechanism in meiotic incompetence of porcine growing oocytes.
Title: Analyses of the involvement of PKA regulation mechanism in meiotic incompetence of porcine growing oocytes.

The following PRKAR2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAR2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00466	NM_214258.2 Latest version!	Sus scrofa protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAR2A ( NM_214258.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999423.2 Sus scrofa protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), mRNA.

Price & Availability↑

CloneID	OSe00466
Clone ID Related Accession (Same CDS sequence)	NM_214258.2
Accession Version	NM_214258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Sus scrofa(pig)
Product	cAMP-dependent protein kinase type II-alpha regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB499528.1. On Jun 11, 2010 this sequence version replaced NM_214258.1. ##Evidence-Data-START## Transcript exon combination :: AB499528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02584787, SAMN02584789 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCACA TCCAGATCCC GCCTGGGCTC ACGGAGCTGC TGCAGGGCTA CACCGTGGAG 
GTGCTGCGGC GGCAGCCACC CGACCTGGTC GACTTCGCGG TGGACTACTT CACCCGCCTG 
CGCGAGGCCC GCTCCCGAGC CTCCACCCCA CCCGCCGCCC CTCCTTCCGG CTCCCAGGAT 
CTAGAGCCCA GCTCTGGCCT TGTCACCGAC GCGATCGCGG ACAGCGAGTC GGAGGACGAC 
GAGGACTTGG ACGTTCCAAT TCCTAGCAGA TTTGATCGGC GAGTATCAGT CTGTGCTGAG 
ACCTATAACC CTGATGAGGA AGAGGAAGAT ACTGATCCAA GGGTGATTCA CCCTAAAACT 
GATCAACAAA GATGCAGACT TCAAGAAGCT TGCAAAGATA TTCTTCTTTT CAAAAATCTT 
GATCAGGAAC AACTTTCTCA AGTCCTCGAT GCTATGTTTG AAAGGACAGT CAAAGTTGAT 
GAGCATGTCA TTGACCAAGG AGATGATGGA GACAACTTTT ATGTTATTGA ACGGGGAACC 
TATGATATTT TAGTAACAAA AGATAATCAA ACCCGCTCTG TTGGTCAGTA TGACAACCAT 
GGCAGTTTTG GAGAACTAGC TCTGATGTAC AACACCCCGA GAGCTGCTAC CATTGTCGCC 
ACTTCAGAAG GCTCCCTTTG GGGACTGGAC CGTGTGACTT TTAGAAGAAT CATAGTGAAA 
AACAATGCAA AAAAGAGGAA GATGTTTGAA TCATTTATTG AATCTGTGCC ACTCCTTAAA 
TCACTAGAGG TGTCAGAACG AATGAAGATC GTGGATGTAA TAGGAGAAAA GATCTATAAG 
GATGGAGAGC GCATCATCAC ACAGGGTGAA AAGGCTGATA GCTTTTACAT TATAGAGTCT 
GGTGAAGTGA GCATCTTGAT TAAAAGCAAG ACTAAAGCAA ACAAGGATGG TGGGAACCAG 
GAGGTCGAGA TTGCCCGCTG CCACAAGGGG CAGTACTTTG GAGAGCTTGC ACTGGTCACC 
AACAAACCCA GAGCTGCTTC AGCTTATGCA GTGGGAGATG TCAAATGCTT AGTTATGGAT 
GTACAAGCAT TTGAGAGGCT TCTGGGGCCC TGCATGGACA TCATGAAGAG GAACATCTCA 
CACTATGAGG AACAGCTGGT GAAGATGTTT GGCTCCAGCA TGGATCTGAT CGATCCCGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999423.2
CDS	2..1207
Translation	MSHIQIPPGLTELLQGYTVEVLRRQPPDLVDFAVDYFTRLREARSRASTPPAAPPSGSQDLEPSSGLVTDAIADSESEDDEDLDVPIPSRFDRRVSVCAETYNPDEEEEDTDPRVIHPKTDQQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERTVKVDEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNHGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIKSKTKANKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSMDLIDPGQ

Target ORF information:

RefSeq Version	NM_214258.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214258.2

ORF Insert Sequence:

ATGAGCCACA TCCAGATCCC GCCTGGGCTC ACGGAGCTGC TGCAGGGCTA CACCGTGGAG 
GTGCTGCGGC GGCAGCCACC CGACCTGGTC GACTTCGCGG TGGACTACTT CACCCGCCTG 
CGCGAGGCCC GCTCCCGAGC CTCCACCCCA CCCGCCGCCC CTCCTTCCGG CTCCCAGGAT 
CTAGAGCCCA GCTCTGGCCT TGTCACCGAC GCGATCGCGG ACAGCGAGTC GGAGGACGAC 
GAGGACTTGG ACGTTCCAAT TCCTAGCAGA TTTGATCGGC GAGTATCAGT CTGTGCTGAG 
ACCTATAACC CTGATGAGGA AGAGGAAGAT ACTGATCCAA GGGTGATTCA CCCTAAAACT 
GATCAACAAA GATGCAGACT TCAAGAAGCT TGCAAAGATA TTCTTCTTTT CAAAAATCTT 
GATCAGGAAC AACTTTCTCA AGTCCTCGAT GCTATGTTTG AAAGGACAGT CAAAGTTGAT 
GAGCATGTCA TTGACCAAGG AGATGATGGA GACAACTTTT ATGTTATTGA ACGGGGAACC 
TATGATATTT TAGTAACAAA AGATAATCAA ACCCGCTCTG TTGGTCAGTA TGACAACCAT 
GGCAGTTTTG GAGAACTAGC TCTGATGTAC AACACCCCGA GAGCTGCTAC CATTGTCGCC 
ACTTCAGAAG GCTCCCTTTG GGGACTGGAC CGTGTGACTT TTAGAAGAAT CATAGTGAAA 
AACAATGCAA AAAAGAGGAA GATGTTTGAA TCATTTATTG AATCTGTGCC ACTCCTTAAA 
TCACTAGAGG TGTCAGAACG AATGAAGATC GTGGATGTAA TAGGAGAAAA GATCTATAAG 
GATGGAGAGC GCATCATCAC ACAGGGTGAA AAGGCTGATA GCTTTTACAT TATAGAGTCT 
GGTGAAGTGA GCATCTTGAT TAAAAGCAAG ACTAAAGCAA ACAAGGATGG TGGGAACCAG 
GAGGTCGAGA TTGCCCGCTG CCACAAGGGG CAGTACTTTG GAGAGCTTGC ACTGGTCACC 
AACAAACCCA GAGCTGCTTC AGCTTATGCA GTGGGAGATG TCAAATGCTT AGTTATGGAT 
GTACAAGCAT TTGAGAGGCT TCTGGGGCCC TGCATGGACA TCATGAAGAG GAACATCTCA 
CACTATGAGG AACAGCTGGT GAAGATGTTT GGCTCCAGCA TGGATCTGAT CGATCCCGGG 
CAGTAG