LOC658663 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC658663
Entrez Gene ID	658663
Full Name	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_965027.3	XP_970120.2	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like

Pathways from BioSystems
Gene Ontology

The following LOC658663 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC658663 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg06409	XM_965027.3 Latest version!	Tribolium castaneum tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (LOC658663), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC658663 ( XM_965027.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_970120.2 Tribolium castaneum tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (LOC658663), mRNA.

Price & Availability↑

CloneID	OTg06409
Clone ID Related Accession (Same CDS sequence)	XM_965027.3
Accession Version	XM_965027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450165.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_965027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGTAA TCCTAACCTC AAAAATGACA AAAACTACTG AAAAATTAAC TTACGACAAC 
AAAATCATAT TGGCCCCCAT GGTACGTGTT GGTACTCTAC CCATGAGATT GTTGTCGCTG 
GACCACGGGG CCGACATTGT CTACACTGAA GAATTAATTG ACTGGAAATT TTTAAAATCA 
ATAAGACGGG AAAATGACGT GTTGGGCACA GTTGATTATC TGGATAAATC AGATGGGACT 
GTAGTGTTCC GAACTTGCCC AAGAGAGAAA AACAGAGTGG TGGTGCAATT GGGGACGTGC 
GACCCACAAA GAGCCTTAAA AGTTGCCAAA ATGATCGAAA ATGATGTAGC TGGGATTGAT 
ATAAACATGG GTTGTCCCAA AGAATTTTCA CTGAAAGGGG GAATGGGGGC TGCTTTGTTA 
AAACAGCCAG AGAAGGCGAA GTCAATTTTA TCCACACTTA CGCAAAATGT AAAAATTCCA 
ATTACGTGCA AAATTCGTGT TTTTGAAAGT GCTGAGGAAA CCTTAGATTT GGTCAAAGAA 
TTGGTGTCAA CGGGTATTTC AGCTATTGCA ATTCATGGAA GAACCAAAGC TGAAAGGCCT 
CAACATCCCA ACAGAAACGC AACAATCAAA TTTATTGCAG AGAACGTATC AATCCCGGTT 
GTTGCCAATG GTGGGTCCAA GGAAATTGAA AAATATCAAG ACATAATTAA ATTTAAGGAA 
GAATGTGGCT GTTCAAGTGT GATGATCGCA AGGGCTGCAG AAGCCAATTG TTCAATTTTT 
CGTAAACAGG GTTTATTAGA TATCGAGACT GTTATAGAAC AGTACCTCAA ATATGCCATT 
GATTATGATA ACTCGCCCAG TAATACAAAA TACTGCATAC AAAACATACT GAAAGAACTT 
CAGGAAACAC CCAGAGGGAA GAAATTTTTA GAATGTCAAA CACTGGAACA AATTTGTGCA 
ATATGGGACC TAGCCGATTA CTGCCGAAAA AAACAACTGC AGTTCCACTC TCAGGGAATT 
TTGAGTCGCC GAGAAATAAC ACCAGATATG TTCAGTCCCA AAGCCAAAAA AGTTAAGACT 
GAATTAGACG ATGACGTAAT CGAAATGAAA TGTGCCTTTA TTAGGGTAAA TTATCCAGAC 
GATCCAGATT TGCCGAAATC AAAAATAATA GCATATTGTG GCAAACACAA GTTTGATTTG 
CCTAAGTACC AGACCGTCAA TGAAGATAAA TTATTTCGCG CAATTGCCAC ATTCCAAGGG 
AAAAAATACA GCTCATCGTA TTGGGAAAAA AATAAACGCT TTGCTGAACA GGGGGCTGCT 
TTGGTCCTGG CCTGTTCTTT AGGTTTAGTT ACGAAAGAGG AATTAATTAA AGATGGAAGC 
ATGTTGGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_970120.2
CDS	137..1528
Translation	MTVILTSKMTKTTEKLTYDNKIILAPMVRVGTLPMRLLSLDHGADIVYTEELIDWKFLKSIRRENDVLGTVDYLDKSDGTVVFRTCPREKNRVVVQLGTCDPQRALKVAKMIENDVAGIDINMGCPKEFSLKGGMGAALLKQPEKAKSILSTLTQNVKIPITCKIRVFESAEETLDLVKELVSTGISAIAIHGRTKAERPQHPNRNATIKFIAENVSIPVVANGGSKEIEKYQDIIKFKEECGCSSVMIARAAEANCSIFRKQGLLDIETVIEQYLKYAIDYDNSPSNTKYCIQNILKELQETPRGKKFLECQTLEQICAIWDLADYCRKKQLQFHSQGILSRREITPDMFSPKAKKVKTELDDDVIEMKCAFIRVNYPDDPDLPKSKIIAYCGKHKFDLPKYQTVNEDKLFRAIATFQGKKYSSSYWEKNKRFAEQGAALVLACSLGLVTKEELIKDGSMLE

Target ORF information:

RefSeq Version	XM_965027.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (LOC658663), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_965027.3

ORF Insert Sequence:

ATGACAGTAA TCCTAACCTC AAAAATGACA AAAACTACTG AAAAATTAAC TTACGACAAC 
AAAATCATAT TGGCCCCCAT GGTACGTGTT GGTACTCTAC CCATGAGATT GTTGTCGCTG 
GACCACGGGG CCGACATTGT CTACACTGAA GAATTAATTG ACTGGAAATT TTTAAAATCA 
ATAAGACGGG AAAATGACGT GTTGGGCACA GTTGATTATC TGGATAAATC AGATGGGACT 
GTAGTGTTCC GAACTTGCCC AAGAGAGAAA AACAGAGTGG TGGTGCAATT GGGGACGTGC 
GACCCACAAA GAGCCTTAAA AGTTGCCAAA ATGATCGAAA ATGATGTAGC TGGGATTGAT 
ATAAACATGG GTTGTCCCAA AGAATTTTCA CTGAAAGGGG GAATGGGGGC TGCTTTGTTA 
AAACAGCCAG AGAAGGCGAA GTCAATTTTA TCCACACTTA CGCAAAATGT AAAAATTCCA 
ATTACGTGCA AAATTCGTGT TTTTGAAAGT GCTGAGGAAA CCTTAGATTT GGTCAAAGAA 
TTGGTGTCAA CGGGTATTTC AGCTATTGCA ATTCATGGAA GAACCAAAGC TGAAAGGCCT 
CAACATCCCA ACAGAAACGC AACAATCAAA TTTATTGCAG AGAACGTATC AATCCCGGTT 
GTTGCCAATG GTGGGTCCAA GGAAATTGAA AAATATCAAG ACATAATTAA ATTTAAGGAA 
GAATGTGGCT GTTCAAGTGT GATGATCGCA AGGGCTGCAG AAGCCAATTG TTCAATTTTT 
CGTAAACAGG GTTTATTAGA TATCGAGACT GTTATAGAAC AGTACCTCAA ATATGCCATT 
GATTATGATA ACTCGCCCAG TAATACAAAA TACTGCATAC AAAACATACT GAAAGAACTT 
CAGGAAACAC CCAGAGGGAA GAAATTTTTA GAATGTCAAA CACTGGAACA AATTTGTGCA 
ATATGGGACC TAGCCGATTA CTGCCGAAAA AAACAACTGC AGTTCCACTC TCAGGGAATT 
TTGAGTCGCC GAGAAATAAC ACCAGATATG TTCAGTCCCA AAGCCAAAAA AGTTAAGACT 
GAATTAGACG ATGACGTAAT CGAAATGAAA TGTGCCTTTA TTAGGGTAAA TTATCCAGAC 
GATCCAGATT TGCCGAAATC AAAAATAATA GCATATTGTG GCAAACACAA GTTTGATTTG 
CCTAAGTACC AGACCGTCAA TGAAGATAAA TTATTTCGCG CAATTGCCAC ATTCCAAGGG 
AAAAAATACA GCTCATCGTA TTGGGAAAAA AATAAACGCT TTGCTGAACA GGGGGCTGCT 
TTGGTCCTGG CCTGTTCTTT AGGTTTAGTT ACGAAAGAGG AATTAATTAA AGATGGAAGC 
ATGTTGGAAT AA