LOC660963 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC660963
Entrez Gene ID	660963
Full Name	lariat debranching enzyme
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_967157.3	XP_972250.1	lariat debranching enzyme

Pathways from BioSystems
Gene Ontology

The following LOC660963 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC660963 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg03474	XM_967157.3 Latest version!	Tribolium castaneum lariat debranching enzyme (LOC660963), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC660963 ( XM_967157.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_972250.1 Tribolium castaneum lariat debranching enzyme (LOC660963), mRNA.

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CloneID	OTg03474
Clone ID Related Accession (Same CDS sequence)	XM_967157.3
Accession Version	XM_967157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	lariat debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450144.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_967157.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTG CGGTCGAAGG TTGTGCCCAC GGCGAATTGG AAACAATTTA CGACACTCTG 
CAATATTTGG AAGAGAAGGA AGGCTTTAAA ATCGATTTGC TCATTTGTTG TGGCGACTTT 
CAAGCTTCTC GCAATGAAGC GGACCTCCGG TGCATGGCGG TGCCTCCTAA ATACTACAAC 
ATTTGTTCAT TTTACAAGTA TTACACCGGG GTGAAAACAG CCCCAATTCT CACGATTTTT 
ATTGGGGGCA ACCACGAAGC GTCCAATTAT CTGCAAGAGT TGCCATATGG GGGCTGGGTG 
GCCCCGAACA TCTATTACTT GGGTTATGCC GGAGTGGTCA ATATTGCAGG TTTAAGAATT 
GGGGGCATTT CGGGGATTTA CAAAGGACAG GATTACATGA AAGGCCATTT TGAGCATTCG 
CCTTACAGCG ATAGTTCAAA ACGCAGCGTT TATCACATCA GAAATTTGGA AGTTTTTCGA 
TTGAAACAAC TGTCTCAACC TTTGGATATT TTTCTGTCAC ATGATTGGCC CTCGGGGATC 
TGGCAATATG GAAATGCGGC GCAATTAATA CGTTTTAAGC CTTTTTTCGA AGAAGATATC 
AACAGAGGAC AGCTGGGTAG CAAACCAACC GAAGAATTGC TAAATCACTT GAAGCCCGAT 
TATTGGTTTT CGGCTCATTT GCACTGTAAA TTTGCCGCAG TGGTGCCACA TGAGTGTGGC 
AAAATCACCA AATTTTTGGC ACTTGATAAG TGTCTTCCTA AAAGAAGGTT TCTGCAAGTT 
GTTGATGTAC CTCATGACAC GAATTTGGAA AAAATTGAAA TTAGTTACGA TCTAGAATGG 
TTGACTATTT TAAGATTAAC AAATCATCTG CTAAGTGTTA AAAATGTGAC CAATTATTTA 
CCAGGGCCTA GTGGCAATGA GAGGTGGTGC TTTTTTCCAG ACGAGGAGGA AAAATCGTTT 
ACTTTGAACC TGTTTGGTGA TCTGGTGGTT CCAAACAATT TCGAGAAAAC AGCTGAAGGT 
TATTCCGGTC ATAAGACTAA AAATTTAACG CAACCAGTGC CAGTTTGTAA TTCACAGACC 
GTTTTGTTTT GTAAAAAATT GGGAATCGAT GATCCGTTGA ATTTAATTCT TAATTTAAGT 
ACAAACTCTG CAATTGATCT TAGTGCAAAT AGTTTAGGTG ATGATAGTGC AAGTAAGACA 
ACACCGAGAA AAATAGTCTT GGACTTGCCC CAACCAGTGA ATGAAAGTGT TCCAACGTTT 
GTGGACGAAA CAATTTCTCA ACCGGAATTG AACGAAACTG TTGAAGAAAT GGAAGTTCCG 
GTTGAGGAAA AATCAAATTG CGGTAAAGAA GTGGAAGTTT CGGTTGAGGA AAAATCAAAC 
TGCGGTAAAG CGTTTAAGAG GAGGAATGCA TCAATGTATC AAAATGATGT AAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_972250.1
CDS	98..1534
Translation	MKIAVEGCAHGELETIYDTLQYLEEKEGFKIDLLICCGDFQASRNEADLRCMAVPPKYYNICSFYKYYTGVKTAPILTIFIGGNHEASNYLQELPYGGWVAPNIYYLGYAGVVNIAGLRIGGISGIYKGQDYMKGHFEHSPYSDSSKRSVYHIRNLEVFRLKQLSQPLDIFLSHDWPSGIWQYGNAAQLIRFKPFFEEDINRGQLGSKPTEELLNHLKPDYWFSAHLHCKFAAVVPHECGKITKFLALDKCLPKRRFLQVVDVPHDTNLEKIEISYDLEWLTILRLTNHLLSVKNVTNYLPGPSGNERWCFFPDEEEKSFTLNLFGDLVVPNNFEKTAEGYSGHKTKNLTQPVPVCNSQTVLFCKKLGIDDPLNLILNLSTNSAIDLSANSLGDDSASKTTPRKIVLDLPQPVNESVPTFVDETISQPELNETVEEMEVPVEEKSNCGKEVEVSVEEKSNCGKAFKRRNASMYQNDVN

Target ORF information:

RefSeq Version	XM_967157.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum lariat debranching enzyme (LOC660963), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_967157.3

ORF Insert Sequence:

ATGAAAATTG CGGTCGAAGG TTGTGCCCAC GGCGAATTGG AAACAATTTA CGACACTCTG 
CAATATTTGG AAGAGAAGGA AGGCTTTAAA ATCGATTTGC TCATTTGTTG TGGCGACTTT 
CAAGCTTCTC GCAATGAAGC GGACCTCCGG TGCATGGCGG TGCCTCCTAA ATACTACAAC 
ATTTGTTCAT TTTACAAGTA TTACACCGGG GTGAAAACAG CCCCAATTCT CACGATTTTT 
ATTGGGGGCA ACCACGAAGC GTCCAATTAT CTGCAAGAGT TGCCATATGG GGGCTGGGTG 
GCCCCGAACA TCTATTACTT GGGTTATGCC GGAGTGGTCA ATATTGCAGG TTTAAGAATT 
GGGGGCATTT CGGGGATTTA CAAAGGACAG GATTACATGA AAGGCCATTT TGAGCATTCG 
CCTTACAGCG ATAGTTCAAA ACGCAGCGTT TATCACATCA GAAATTTGGA AGTTTTTCGA 
TTGAAACAAC TGTCTCAACC TTTGGATATT TTTCTGTCAC ATGATTGGCC CTCGGGGATC 
TGGCAATATG GAAATGCGGC GCAATTAATA CGTTTTAAGC CTTTTTTCGA AGAAGATATC 
AACAGAGGAC AGCTGGGTAG CAAACCAACC GAAGAATTGC TAAATCACTT GAAGCCCGAT 
TATTGGTTTT CGGCTCATTT GCACTGTAAA TTTGCCGCAG TGGTGCCACA TGAGTGTGGC 
AAAATCACCA AATTTTTGGC ACTTGATAAG TGTCTTCCTA AAAGAAGGTT TCTGCAAGTT 
GTTGATGTAC CTCATGACAC GAATTTGGAA AAAATTGAAA TTAGTTACGA TCTAGAATGG 
TTGACTATTT TAAGATTAAC AAATCATCTG CTAAGTGTTA AAAATGTGAC CAATTATTTA 
CCAGGGCCTA GTGGCAATGA GAGGTGGTGC TTTTTTCCAG ACGAGGAGGA AAAATCGTTT 
ACTTTGAACC TGTTTGGTGA TCTGGTGGTT CCAAACAATT TCGAGAAAAC AGCTGAAGGT 
TATTCCGGTC ATAAGACTAA AAATTTAACG CAACCAGTGC CAGTTTGTAA TTCACAGACC 
GTTTTGTTTT GTAAAAAATT GGGAATCGAT GATCCGTTGA ATTTAATTCT TAATTTAAGT 
ACAAACTCTG CAATTGATCT TAGTGCAAAT AGTTTAGGTG ATGATAGTGC AAGTAAGACA 
ACACCGAGAA AAATAGTCTT GGACTTGCCC CAACCAGTGA ATGAAAGTGT TCCAACGTTT 
GTGGACGAAA CAATTTCTCA ACCGGAATTG AACGAAACTG TTGAAGAAAT GGAAGTTCCG 
GTTGAGGAAA AATCAAATTG CGGTAAAGAA GTGGAAGTTT CGGTTGAGGA AAAATCAAAC 
TGCGGTAAAG CGTTTAAGAG GAGGAATGCA TCAATGTATC AAAATGATGT AAATTGA