| Service Specifications | |
|---|---|
| Oligo Length | Up to 170 bases |
| Yield | Up to 1 fmol of each oligo |
| Error Rate | ~0.5% |
| Minimum order | None |
| Turnaround Time | 1-2 weeks |
| Service Specifications | |
|---|---|
| Oligo Length | Up to 170 bases |
| Yield | Up to 1 fmol of each oligo |
| Error Rate | ~0.5% |
| Minimum order | None |
| Turnaround Time | 1-2 weeks |
Pricing is based on chip size and oligo length. We offer two different chip capacities. Need a larger number of oligos? You can combine oligos from multiple chips into a single pool to meet your specific experimental needs.
Our no minimum order size and simple pricing structure makes oligo synthesis affordable, allowing you to quickly and efficiently get what you need!
| PRICING | ||
|---|---|---|
| Oligo Length (Bases)* |
Up to 12,472 oligos (USD) #SC1966-12 |
Up to 91,766 oligos (USD) #SC1966-92 |
| 10-79 | $1600 | $4000 |
| 80-109 | $1800 | $4500 |
| 110-130 | $2000 | $5000 |
| 131-150 | $2200 | $5500 |
| 151-170 | $2400 | $6000 |
*If you need oligos of 171-200 bases in length, please contact us for pricing and availability. Pricing is determined by the longest oligo length in the pool. We recommend keeping the lengths of the oligos in your pool within 10%.
The semiconductor-based chip technology used for oligo pool generation allows for automated control of the synthesis process. Each electrode is individually monitored, generating oligos of the highest quality with top notch sequence accuracy. Performance of all electrodes are verified and logged for each pool.
New – Synthesis verification of full oligo length using spike-in control oligos
In order to verify full-length synthesis of oligos, we now offer an additional QC option for FREE, PCR amplification of control oligos synthesized on the same chip . Sequences of 50 optimized control oligos will be synthesized with each pool and PCR amplification of the control oligos will be performed to confirm oligo synthesis. Customer sequences will be screened to ensure QC PCR primers will only capture control oligos.
Our standard quality control includes:
*PCR amplification of entire pool, cloning, and sequencing for sequence coverage and accuracy at an additional fee*
Please contact our customer service representatives to get guaranteed final amount of your desired oligo pool:
Email: oligo@genscript.com
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262
Synthesis Verification of Full Length Oligos in a Pool
Uniform Distribution of 12,000 Unique Oligos
The customer ordered a 12,000 125 nt oligo pool. By using our proprietary semi-conductor array synthesis platform, we delivered the product within 7 days. Post synthesis we amplified the oligo pool and performed NGS via illumina Hi-Seq. Results are shown in the figure to the left.
Building a diverse CRISPR gRNA library using arrayed-synthesized oligo pools
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Double stranded DNA capture probes in NGS targeted sequencing
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Assembly of precise mutant libraries using arrayed-synthesized oligos
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Cost-effective library construction and efficient high-throughput screening
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Oligo pools are a collection of thousands of oligonucleotides
Free Download Free DownloadCheck out our interview from the TIDES: Oligonucleotide & Peptide Therapeutics 2019 conference featuring our senior scientist, Marcelo Caraballo! Learn more about our chip-based oligo pool synthesis technology and developing innovative solutions for the precision diagnostic and therapeutic research fields.
Learn MoreOligo pools have a wide array of uses in various research applications. Click below to learn more about how GenScript’s oligo pool and related services can meet your application needs.
CRISPR gRNA library
gRNA libraries are ideal for high-throughput screening of important molecular targets for developing cancer therapeutics and diagnostics as well as microorganism engineering. These libraries leverage the efficiency and specificity of the CRISPR gene editing technology to either knock-out gene expression or transcriptionally activate genes in the genome.
shRNA library
A pooled lentiviral screen can be performed to identify genes that regulate cellular responses and signaling pathways or to discover novel gene functions. Pooled screening libraries can consist of as few as 50 constructs up to many thousands. GenScript’s siRNA Design Center and chip based synthesis platform make the lentivirus-based RNAi libraries available to verify your research hypothesis.
Mutant libraries are used to identify critical residues or regulatory elements and optimize protein structure and function. GenScript offers various mutant libraries customized for your application needs, including industrial & therapeutic enzymes, metabolic & protein engineering and antibody drug development. Our oligo pool synthesis platform allows full control of mutation ratio and creates near 100% coverage of desired variants.
Library services we offer:
Molecular diagnostics based on NGS technology has shown great potential to revolutionize the in vitro diagnostics market. Selectively capturing genomic regions of interest from a DNA sample prior to sequencing reduces the expense of whole genome sequencing and speeds up the discovery process. GenScript’s oligo pool synthesis platform allows high-throughput synthesis of oligos, providing a fast and economic approach to discovery of disease causing mutations or optimization of gene panels in diagnostic kit development.
Synthetic biology seeks to make the design, construction, and optimization of biological systems easier, more predictable and reliable. This requires the assembly of DNA circuitry, which can be costly, time-consuming, and complex. GenScript’s oligo pool service allows parallel oligo construction of high sequence fidelity oligos on a large scale, making the creation of synthetic genes and construction of optimized biological systems happen quickly and cost-effectively.
Our technical account managers will contact you within 24 hours
Orders can be placed by phone, email, fax, or online with a formal PO (purchase order) or credit card
Our customer service representatives are available 24 hours Monday through Friday.
