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Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR-Cas9 and ssODN in human cancer cells.

Cell Biosci. 2018;

HuZheng，ShiZhaoying，GuoXiaogang，JiangBaishan，WangGuo，LuoDixian，ChenYonglong，ZhuYuan-

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Gene Synthesis	… Methods. Oligonucleotides, primers and ligase IV inhibitor. All ssODNs used for transfection studies were manufactured by GenScript (Nanjing, China). Primers used for PCR and oligonucleotides used for annealing were synthesized by GIGA Biotechnology (Guangzhou, China) …	Get A Quote

Abstract

Precise genome editing is essential for both basic and translational research. The recently developed CRISPR/Cas9 system can specifically cleave a designated site of target gene to create a DNA double-strand break， which triggers cellular DNA repair mechanism of either inaccurate non-homologous end joining， or site-specific homologous recombination. Unfortunately， homology-directed repair (HDR) is challenging due to its very low efficiency. Herein， we focused on improving the efficiency of HDR using a combination of CRISPR/Cas9， eGFP， DNA ligase IV inhibitor SCR7， and single-stranded oligodeoxynucleotides (ssODN) in human cancer cells.

Keywords

CRISPR/Cas9，DNA ligase IV inhibitor，Homology-directed repair，Non-homologous DNA end joining，Single-stranded oligodeoxynucleotides (ss

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