In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes.

In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.

Digoxigenin labeling; Gene expression; In situ hybridization; Paraffin section; RNA probe; RNase-free; mRNA