Resources » Reference Databases » Citations Database

For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

3D culture system to study collective migration in mammary organoid epithelium

STAR Protoc. 2021-08;

Yunzhe Lu, Ruolan Deng, Huanyang You, Pengfei Lu

Products/Services Used	Details	Operation
Recombinant Proteins	We have compared FGF10 provided by different manufacturers and have found that rmFGF10 (GenScript, Cat#Z03155) worked well.	Get A Quote

Abstract

We recently established an culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach. The technical difficulties in conducting the 3D migration assay are choosing epithelial organoids of appropriate sizes and manually paring organoids and beads pre-soaked in FGF10 within a desirable distance (∼100 μm). For complete details on the use and execution of this protocol, please refer to Lu et al. (2020).

Keywords

Cell Biology, Cell culture, Developmental biology, Microscopy, Organoids

Article Link >

Most Popular Services

Homology-Directed Repair (HDR) Knock-in Templates

CRISPR Cas9 Single guide RNA (sgRNA) and Ribonucleoprotein (RNP)

Plasmid DNA Preparation Service

Mammalian CHO Expression Service