The accurate determination of an antibody’s sequence is crucial for various applications, including monoclonal antibody (mAb) engineering, functional optimization, database banking, and patent applications. To prevent the loss of genetic information, sequencing serves as a powerful tool for identifying antibodies produced by hybridoma cells.
For efficient and reliable hybridoma antibody sequencing, our ISO9001-certified service is your trusted choice. We offer two professional production sites, located in CN and US, ensuring prompt and efficient delivery. With a turnaround time as fast as 8 BDs, we guarantee the delivery of native antibody sequence information with no mutations or contamination caused by sample preparation.
In addition, our TurboCHO™ antibody expression platform complements the hybridoma sequencing service by providing purified antibodies as fast as 5 BDs. This combination of services allows us to quickly provide you with valuable insights into antibody diversity, facilitating the development of recombinant antibodies. These insights contribute significantly to advancements in antibody-based research and therapeutics.
Unparalleled
Turnaround Time
Diverse & High-Quality
Delivery
One-Stop Solution
for Ab Development
Cells (hybridoma cells, B cells, single B cells)
| Service Packages | Delivery Timeline Updated (starts from) |
Default Deliverables | Optional Deliverables | |
|---|---|---|---|---|
| Sanger | Antibody Variable Domain | CN Site: 10 BDs US Site: 8 BDS * |
Sequencing report including:
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| Full-Length Antibody | CN Site: 12 BDs US Site: 10 BDS * |
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| NGS | High-throughput Sequencing |
CN Site: 10 BDs US Site: unavailable |
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Note:
a)*: For shipping time, the US site is ~1 week faster than the CN site.
b) Sample request:
CN site: Amount: >1×106 cells; Species: human, mouse, rat, rabbit, ferret, etc.
US site: Amount: >1×106 cells; Species: mouse, rat, etc.
c) Additional services: 1. high-throughput recombinant
antibody expression 2. hybridoma antibody
production 3. antigen-antibody binding confirmation
Goal: Develop antibodies specific to 4-1BB
Immunization: 4-1BB extracellular region
Result: 107 4-1BB antibodies were obtained and the genome information was analyzed by the high-throughput NGS antibody sequencing platform
1. Phylogenetic Tree Analysis - VH Region
Out of 107 clones, 5 clones show the identical V-region with other clones
2. Phylogenetic Tree Analysis - VH Region
Out of 107 clones, 6 clones show the identical V-region with other clones
3. Germline Analysis (Indicates antibody diversity)
4. CDR Length Analysis (Indicates antibody candidate)
From gene to protein, as fast as 5 BDs
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In vivo and in vitro antibody production from hybridoma cells
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Develop mouse and rabbit mAb to best suit your needs
Learn MoreWhat is the principle of hybridoma cell sequencing?
Hybridoma sequencing involves amplifying and sequencing the cDNA encoding the VH and VL domains from a hybridoma cell line. By utilizing high-throughput sequencing technologies, this technique enables the rapid and efficient determination of antibody gene sequences.
What is the principle of hybridoma cell sequencing?
1. Preserved Antibody Diversity: Hybridoma cell sequencing allows for the sequencing of antibodies produced by hybridoma cells, which are derived from a specific B cell clone. This preserves the natural diversity present in the immune response. In contrast, de novo antibody sequencing relies on isolating individual antibodies and may not capture the full diversity present in the immune response.
2. Time and Cost Efficiency: Hybridoma cell sequencing can be a more time and cost-efficient approach compared to de novo antibody sequencing.
3. Quality Control and Validation: Since the sequences are obtained from the hybridoma cells themselves, it ensures that the sequenced antibodies are indeed the ones produced by the hybridoma clone of interest. In de novo sequencing, additional validation steps are required to confirm the authenticity of the obtained sequences.
4. Compatibility with Established Hybridoma Lines: The hybridoma cell lines may have a known history, desired properties, and a documented track record of antibody production. Sequencing the antibody genes from these hybridomas provides a wealth of information about their antibodies, aiding in further characterization and development.
5. Distinguishing certain amino acids: Protein sequencing can encounter challenges in differentiating specific amino acids, such as leucine and isoleucine, which may introduce errors in the final sequence. In contrast, hybridoma sequencing directly sequences the cDNA of the antibody-producing cells, eliminating this potential issue and yielding a more precise, accurate, and reliable result.
How to choose the antibody sequencing method, Sanger or NGS?
1. Research Objectives: If you require the full-length coding sequence of the antibody, including the variable region and constant region, Sanger sequencing may be a suitable choice. However, if you need a comprehensive analysis of the antibody repertoire, including clonal diversity and identification of rare or low-abundance variants, NGS can provide a more comprehensive picture.
2. Sample Quantity: Assess the quantity of samples you need to sequence. Sanger sequencing is generally suitable for a smaller number of samples due to its labor-intensive and costly nature. On the other hand, NGS platforms offer higher throughput, enabling the sequencing of a larger number of samples simultaneously.
3. Cost Considerations: Evaluate your budget and resources. Sanger sequencing is often more cost-effective for smaller-scale projects or when sequencing a limited number of samples. NGS can be more expensive due to the higher upfront costs associated with library preparation, sequencing, and data analysis. However, for large-scale projects or when sequencing multiple samples, NGS can provide cost advantages.
4. Sequence Accuracy: Assess the level of sequence accuracy required for your research. Sanger sequencing has a long-standing reputation for high accuracy, particularly in obtaining individual full-length sequences. NGS platforms, while generally accurate, may have higher error rates, especially in homopolymeric regions or repetitive sequences. Consider whether the specific accuracy requirements of your project can be met by either method.
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