The accurate determination of an antibody’s sequence is crucial for various applications, including monoclonal antibody (mAb) engineering, functional optimization, database banking, and patent applications. To prevent the loss of genetic information, sequencing serves as a powerful tool for identifying antibodies produced by hybridoma cells.

For efficient and reliable hybridoma antibody sequencing, our ISO9001-certified service is your trusted choice. We offer two professional production sites, located in CN and US, ensuring prompt and efficient delivery. With a turnaround time as fast as 8 BDs, we guarantee the delivery of native antibody sequence information with no mutations or contamination caused by sample preparation.

In addition, our TurboCHO™ antibody expression platform complements the hybridoma sequencing service by providing purified antibodies as fast as 5 BDs. This combination of services allows us to quickly provide you with valuable insights into antibody diversity, facilitating the development of recombinant antibodies. These insights contribute significantly to advancements in antibody-based research and therapeutics.

Service Highlights

Unparalleled Turnaround Time

Turnaround Time

  • Deliver antibody sequence as fast as 8 BDs
  • High-throughput sequencing with comprehensive genomic analysis
Diverse & High-Quality Delivery

Diverse & High-Quality

  • Multiple species (human, mouse, rat, rabbit, etc.) and isotypes (IgG, IgM, etc.)
  • Variable region or full-length antibody sequencing
One-Stop Solution for Ab Development

One-Stop Solution
for Ab Development

  • Experienced primer design and antibody gene analysis capabilities
  • Multiple antibody production technologies

Antibody Sequencing Workflow

Cells (hybridoma cells, B cells, single B cells)

 Antibody Sequencing Workflow

Service Details

Service Packages Delivery Timeline Updated
(starts from)
Default Deliverables Optional Deliverables
Sanger Antibody Variable Domain CN Site: 10 BDs
US Site: 8 BDS *
 Sequencing report including:
  • Variable region/full-length nucleotide sequences
  • Variable region/full-length amino acid sequences
  • FR and CDR analysis within the variable region
  • V(D)J gene analysis
  • Sequencing plasmids containing antibody coding sequence
  • Expression construct containing antibody coding sequence of light/heavy chain
Full-Length Antibody CN Site: 12 BDs
US Site: 10 BDS *
NGS High-throughput Sequencing CN Site: 10 BDs
US Site: unavailable
  • DNA and Amino Acid sequences
  • CDR and FR regions are defined by Kabat rules
  • Count number of the sequences obtained by NGS
  • Phylogenetic tree analysis
  • Sequence alignment of variable regions or specific CDR sequences
  • Unique sequences to exclude the similarity sequences (Clients can require at least 2 or more different amino acids contained)
  • CDR length analysis
  • Germline analysis
  • Other analyses can be achieved by our bioinformaticians

a)*: For shipping time, the US site is ~1 week faster than the CN site.
b) Sample request:
CN site: Amount: >1×106 cells; Species: human, mouse, rat, rabbit, ferret, etc.
US site: Amount: >1×106 cells; Species: mouse, rat, etc.
c) Additional services: 1. high-throughput recombinant antibody expression 2. hybridoma antibody production 3. antigen-antibody binding confirmation

Case Studies

Additional Services

Guaranteed PolyExpress™
Recombinant Antibody Production with TurboCHO™ System

From gene to protein, as fast as 5 BDs

Learn More
Guaranteed PolyExpress™
Hybridoma Antibody Production

In vivo and in vitro antibody production from hybridoma cells

Learn More
Guaranteed PolyExpress™
Monoclonal Antibody Development

Develop mouse and rabbit mAb to best suit your needs

Learn More


What is the principle of hybridoma cell sequencing?

Hybridoma sequencing involves amplifying and sequencing the cDNA encoding the VH and VL domains from a hybridoma cell line. By utilizing high-throughput sequencing technologies, this technique enables the rapid and efficient determination of antibody gene sequences.

What is the principle of hybridoma cell sequencing?

1. Preserved Antibody Diversity: Hybridoma cell sequencing allows for the sequencing of antibodies produced by hybridoma cells, which are derived from a specific B cell clone. This preserves the natural diversity present in the immune response. In contrast, de novo antibody sequencing relies on isolating individual antibodies and may not capture the full diversity present in the immune response.

2. Time and Cost Efficiency: Hybridoma cell sequencing can be a more time and cost-efficient approach compared to de novo antibody sequencing.

3. Quality Control and Validation: Since the sequences are obtained from the hybridoma cells themselves, it ensures that the sequenced antibodies are indeed the ones produced by the hybridoma clone of interest. In de novo sequencing, additional validation steps are required to confirm the authenticity of the obtained sequences.

4. Compatibility with Established Hybridoma Lines: The hybridoma cell lines may have a known history, desired properties, and a documented track record of antibody production. Sequencing the antibody genes from these hybridomas provides a wealth of information about their antibodies, aiding in further characterization and development.

5. Distinguishing certain amino acids: Protein sequencing can encounter challenges in differentiating specific amino acids, such as leucine and isoleucine, which may introduce errors in the final sequence. In contrast, hybridoma sequencing directly sequences the cDNA of the antibody-producing cells, eliminating this potential issue and yielding a more precise, accurate, and reliable result.

How to choose the antibody sequencing method, Sanger or NGS?

1. Research Objectives: If you require the full-length coding sequence of the antibody, including the variable region and constant region, Sanger sequencing may be a suitable choice. However, if you need a comprehensive analysis of the antibody repertoire, including clonal diversity and identification of rare or low-abundance variants, NGS can provide a more comprehensive picture.

2. Sample Quantity: Assess the quantity of samples you need to sequence. Sanger sequencing is generally suitable for a smaller number of samples due to its labor-intensive and costly nature. On the other hand, NGS platforms offer higher throughput, enabling the sequencing of a larger number of samples simultaneously.

3. Cost Considerations: Evaluate your budget and resources. Sanger sequencing is often more cost-effective for smaller-scale projects or when sequencing a limited number of samples. NGS can be more expensive due to the higher upfront costs associated with library preparation, sequencing, and data analysis. However, for large-scale projects or when sequencing multiple samples, NGS can provide cost advantages.

4. Sequence Accuracy: Assess the level of sequence accuracy required for your research. Sanger sequencing has a long-standing reputation for high accuracy, particularly in obtaining individual full-length sequences. NGS platforms, while generally accurate, may have higher error rates, especially in homopolymeric regions or repetitive sequences. Consider whether the specific accuracy requirements of your project can be met by either method.

Get in Touch
with GenScript Custom
Antibody Services

Get in Touch with GenScript Custom Antibody Services
Please enter a valid first name.
Please enter a valid last name.
Please enter a valid email name.
Please select a valid industry.
Please select a valid country.
Please enter a valid Zipcode.
Please enter a valid institution/company.
Please enter a message in the textarea.

Quotation and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

Fill out the online form and email to [email protected]
A Technical Account Manager will reply you within 24 hours to finalize the quote
To place order, provide credit card/PO information
Production of your project begins immediately
A Project Manager is in contact with you during the production process to give updates



Do you like the current new website?