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sgrna

sgRNA stands for "single guide RNA" or “small guide RNA”. It is a type of RNA molecule used in the CRISPR-Cas9 gene editing system, which is a powerful tool for making precise changes to DNA sequences in living cells.

In the CRISPR-Cas9 system, the sgRNA molecule is designed to specifically bind to a target DNA sequence and guide the Cas9 protein to that location. Once the Cas9 protein is attached to the DNA, it can cut the DNA at the desired location, which allows for the addition, removal, or replacement of specific DNA sequences.

The design of the sgRNA molecule is a critical step in the CRISPR-Cas9 gene editing process. It must be engineered to specifically recognize and bind to the target DNA sequence while avoiding unintended off-target effects. The sgRNA typically consists of a short sequence of RNA called the guide sequence, which is complementary to the target DNA sequence, as well as other RNA sequences necessary for proper function within the CRISPR-Cas9 system.

Related Biology Tools

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Service and Products

CRISPR Services

Improve genome editing efficiency and accuracy with GenScript’s comprehensive CRISPR/Cas9 genome editing services.

Synthetic sgRNA and crRNA Service

Synthetic CRISPR/Cas9 RNA reagents for genome editing with higher cleavage efficiency and fewer off-target effects.

CRISPR Cell Lines

GenScript’s CRISPR customized stable knockout cell line service will create specific gene knockout in your cell line of interest.

Microbial Gene Editing Services

Highly efficient, seamless genome editing in E.coli using CRISPR technology

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