List by Alphabet: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

band shift assay

gel shift, or Band Shift assay, or Electrophoretic mobility shift assay (EMSA) is a technique for studying gene regulation and determining protein:DNA interactions. The assay is based on the observation that complexes of protein and DNA migrate through a non-denaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides. The gel shift assay is carried out by first incubating a protein(s) (such as nuclear or cell extract) with a 32P end-labeled DNA fragment containing the putative protein binding site. The reaction products are then analyzed on a nondenaturing polyacrylamide gel. The specificity of the DNA-binding protein for the putative binding site is established by competition experiments using DNA fragments or oligonucleotides containing a binding site for the protein of interest or other unrelated DNA sequences.


Related Biology Tools

  • GenSmart™ Codon Optimization

    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

    Learn More

  • DNA Construct Design Tool

    GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.

    Learn More

  • Codon Frequency Tables

    This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.

    Learn More

Service and Products

Protein Expression

GenScript recombinant protein and rAb services provide high quality recombinant proteins and rAbs for a variety of downstream research applications.

Bacterial Expression

One-stop service from Sequence to Protein starting from $1600, 4 weeks.

Insect Expression

GenScript's BacuVance baculovirus expression system was developed by our in-house team of scientists for virus production and expression of recombinant proteins from baculovirus-infected insect cells.

Mammalian Transient Expression

Proprietary High Density (HD) expression system, enhance the protein yield up to 100 fold, achieve antibody titers up to 3 g/L.

Reagents for COVID-19 Research

Gram level, ready to ship RBD proteins, ACE2 assay cell lines and pseudovirus.

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]

If your term is adopted, we will send 1,000 EzCoupon points to your GenScript account.
feedback

Do you like the current new website?

Hate

Dislike

Neutral

Like

Love

*