ORF » Species Summary » Cryptococcus neoformans var. grubii H99 » CNAG_05140 cDNA ORF clone

CNAG_05140 cDNA ORF clone, Cryptococcus neoformans var. grubii H99

The following CNAG_05140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNAG_05140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCr135854 XM_012193345.1
Latest version!		 Cryptococcus neoformans var. grubii H99 methylenetetrahydrofolate reductase (CNAG_05140), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CNAG_05140 ( XM_012193345.1 ) cDNA ORF clone, Cryptococcus neoformans var. grubii H99 -> XP_012048735.1 Cryptococcus neoformans var. grubii H99 methylenetetrahydrofolate reductase (CNAG_05140), mRNA.
    Price & Availability↑
    CloneID OCr135854
    Clone ID Related Accession (Same CDS sequence) XM_012193345.1
    Accession Version XM_012193345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-03
    Organism Cryptococcus neoformans var. grubii H99
    Product methylenetetrahydrofolate reductase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026748). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAGATCA CCGAGAAGCT GCAGAAGGCT GAGAAGGAAG GTCGACCCTT CTGGAGCTTT 
    GAATTCTTCC CTCCCCGAAC CGCTCAGGGT TTGCAAAACC TCTATGACCG TATCGAGCGT 
    ATGAGGGATC TTGGCCCAGA GTTTATTGAC ATCACCTGGG GCGCCGGTGG AAAGAATGCC 
    GATCTTACCA ACTCTCTTGT CCAGGTCTGC CAGGCAACCA TCGGTATCGA AACTTGCATG 
    CACCTTTGCT GTACCGAAAT GCCCAAGGAA AAGGTCGAAT GGGCCCTCAA GGAAGCTAAA 
    TCTCATGGGT GTCAAAACAT CCTTGCCCTC CGTGGCGACC CTGTGGCCGG CACATCTACA 
    TGGGAGCCTA CTCCTGGTGG ATTCATGAAC GCCGTCGACC TCGTCCGATA CATTCACAAG 
    CATTACCCCG GAGATTTCTG CGTTGCCGTT GCCGGATTCC CTCAGGGGCA TCCCGAGACT 
    CCCGACACTC CCGAGGGCAG GGCTCAAGAG ATGGAGTGGC TGAAAGCGAA GGTTGATGCA 
    GGAGCCGAAT TTATCTTCAC TCAGATGTTC TACGACACTT CAATCTTCTT TGACTGGGTT 
    AAGCGCGTGA GGGAGGCTGG CATCAATGCT CCCATCATTC CTGGTATCAT GCCTATTCAG 
    AACTGGGAAA AGTTTGAGAA ATGGGTCCAG AGGGAGAATA TTATCGTGCC CTCTCATTTC 
    TATGAGGCTC TTAACCCTGT TCGGGGAGAC GATGAAAAGG TGCGACAGGT TGGGACGAAG 
    TTGGTTGCCG ATATGTGTAA GACAATCTTG GCGAACAAGG AGGCTGGTAT CAAGGGTTTG 
    CACATCTACA CATTGAACTT GGAAAAGGGA GCGAGGATGT TGTTGCAGGA ACTTGGCTTC 
    GAGGGAAGGA GAGAGCAAAT AGCGCCATTG CCATGGAGGC CAAGTTTGAC TCCTAGCCGA 
    AGAGAGGAGA CCATCAGGCC AATCTTCTGG GCCAATCGGA CCCGCTCGTA CCTTTCCAGG 
    ACCGCTGATT GGGATGAATT CCCTAACGGA CGATGGGGTG ATTCCCGAAG TCCTGCATAC 
    GGTGATCTCG ACGGTTATCC TGTATCCATC GGTATTACTG CTCCTGACGC GTATAACCTT 
    TGGGGCCACC CCACTACTTC CTTCGATCTT CACGCCCTCT TCGCTCGTTT CTGCCGTGGT 
    GACTTGGCCA AACTTCCTTG GTCCACCCGA CCTCCTGCCT CTGAGACGTC TCTTATCATT 
    GAACAGCTTG CCAAGATGAA CGAGCTCGGA TACCTTACCA TCAACTCTCA GCCAGCAGTG 
    GATGGTGTGA GGAGCGACGA CAAGGTGCAC GGTTGGGGCC CAGCTGGTGG CTATGTCTAT 
    CAGAAGGCCT ATCTCGAGTT CTTTGTCTCT CCCGACCTGC TTTCTCCTTT GATTAGGAGA 
    ATTGAGAGAG ACCCCAGGAT CACCTACTAT GCTGTCAACA AGCAGGGTGA TCTGAGGACC 
    AACACTCACA GCGAGAGCCC CAACGCTGTT ACTTGGGGTG TCTTCCCCGG CAAGGAAATT 
    GTCCAACCCA CCATCGTGGA AGCGGTCTCT TTCATCGCCT GGAAAGACGA AGCCTTCGAA 
    CTCGGTCTGC AGTGGGCTCA TCTTTACCCC GCTGACTCTC CTTCTCGTAA ACTCATAACC 
    GACACCATGA ACACTTCCTA CCTCGTCAAC ATCGTCGCTA ATGACTTCCG AGATGGCATG 
    TCGATCTTCG AACCTTTCTT GCTTGATCAG ACGGCTGTCG GGAAGGTCGT GGAGGGCGTG 
    AAGGGCACTG TTGAGGGGAT CGTTGAGGGT GTGAAGGGTG TTGTCGGCGC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012048735.1
    CDS415..2268
    Translation

    Target ORF information:

    RefSeq Version XM_012193345.1
    Organism Cryptococcus neoformans var. grubii H99
    Definition Cryptococcus neoformans var. grubii H99 methylenetetrahydrofolate reductase (CNAG_05140), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012193345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAGATCA CCGAGAAGCT GCAGAAGGCT GAGAAGGAAG GTCGACCCTT CTGGAGCTTT 
    GAATTCTTCC CTCCCCGAAC CGCTCAGGGT TTGCAAAACC TCTATGACCG TATCGAGCGT 
    ATGAGGGATC TTGGCCCAGA GTTTATTGAC ATCACCTGGG GCGCCGGTGG AAAGAATGCC 
    GATCTTACCA ACTCTCTTGT CCAGGTCTGC CAGGCAACCA TCGGTATCGA AACTTGCATG 
    CACCTTTGCT GTACCGAAAT GCCCAAGGAA AAGGTCGAAT GGGCCCTCAA GGAAGCTAAA 
    TCTCATGGGT GTCAAAACAT CCTTGCCCTC CGTGGCGACC CTGTGGCCGG CACATCTACA 
    TGGGAGCCTA CTCCTGGTGG ATTCATGAAC GCCGTCGACC TCGTCCGATA CATTCACAAG 
    CATTACCCCG GAGATTTCTG CGTTGCCGTT GCCGGATTCC CTCAGGGGCA TCCCGAGACT 
    CCCGACACTC CCGAGGGCAG GGCTCAAGAG ATGGAGTGGC TGAAAGCGAA GGTTGATGCA 
    GGAGCCGAAT TTATCTTCAC TCAGATGTTC TACGACACTT CAATCTTCTT TGACTGGGTT 
    AAGCGCGTGA GGGAGGCTGG CATCAATGCT CCCATCATTC CTGGTATCAT GCCTATTCAG 
    AACTGGGAAA AGTTTGAGAA ATGGGTCCAG AGGGAGAATA TTATCGTGCC CTCTCATTTC 
    TATGAGGCTC TTAACCCTGT TCGGGGAGAC GATGAAAAGG TGCGACAGGT TGGGACGAAG 
    TTGGTTGCCG ATATGTGTAA GACAATCTTG GCGAACAAGG AGGCTGGTAT CAAGGGTTTG 
    CACATCTACA CATTGAACTT GGAAAAGGGA GCGAGGATGT TGTTGCAGGA ACTTGGCTTC 
    GAGGGAAGGA GAGAGCAAAT AGCGCCATTG CCATGGAGGC CAAGTTTGAC TCCTAGCCGA 
    AGAGAGGAGA CCATCAGGCC AATCTTCTGG GCCAATCGGA CCCGCTCGTA CCTTTCCAGG 
    ACCGCTGATT GGGATGAATT CCCTAACGGA CGATGGGGTG ATTCCCGAAG TCCTGCATAC 
    GGTGATCTCG ACGGTTATCC TGTATCCATC GGTATTACTG CTCCTGACGC GTATAACCTT 
    TGGGGCCACC CCACTACTTC CTTCGATCTT CACGCCCTCT TCGCTCGTTT CTGCCGTGGT 
    GACTTGGCCA AACTTCCTTG GTCCACCCGA CCTCCTGCCT CTGAGACGTC TCTTATCATT 
    GAACAGCTTG CCAAGATGAA CGAGCTCGGA TACCTTACCA TCAACTCTCA GCCAGCAGTG 
    GATGGTGTGA GGAGCGACGA CAAGGTGCAC GGTTGGGGCC CAGCTGGTGG CTATGTCTAT 
    CAGAAGGCCT ATCTCGAGTT CTTTGTCTCT CCCGACCTGC TTTCTCCTTT GATTAGGAGA 
    ATTGAGAGAG ACCCCAGGAT CACCTACTAT GCTGTCAACA AGCAGGGTGA TCTGAGGACC 
    AACACTCACA GCGAGAGCCC CAACGCTGTT ACTTGGGGTG TCTTCCCCGG CAAGGAAATT 
    GTCCAACCCA CCATCGTGGA AGCGGTCTCT TTCATCGCCT GGAAAGACGA AGCCTTCGAA 
    CTCGGTCTGC AGTGGGCTCA TCTTTACCCC GCTGACTCTC CTTCTCGTAA ACTCATAACC 
    GACACCATGA ACACTTCCTA CCTCGTCAAC ATCGTCGCTA ATGACTTCCG AGATGGCATG 
    TCGATCTTCG AACCTTTCTT GCTTGATCAG ACGGCTGTCG GGAAGGTCGT GGAGGGCGTG 
    AAGGGCACTG TTGAGGGGAT CGTTGAGGGT GTGAAGGGTG TTGTCGGCGC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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