CNAG_05298 cDNA ORF clone, Cryptococcus neoformans var. grubii H99

Gene
Clones
gRNAs

Summary

Gene Symbol	CNAG_05298
Entrez Gene ID	23888624
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Cryptococcus neoformans var. grubii H99

mRNA and Protein(s)

mRNA	Protein	Name
XM_012193446.1	XP_012048836.1	hypothetical protein, variant
XM_012193193.1	XP_012048583.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation.
Janbon G, Ormerod KL, Paulet D, Byrnes EJ 3rd, Yadav V, Chatterjee G, Mullapudi N, Hon CC, Billmyre RB, Brunel F, Bahn YS, Chen W, Chen Y, Chow EW, Copp?e JY, Floyd-Averette A, Gaillardin C, Gerik KJ, Goldberg J, Gonzalez-Hilarion S, Gujja S, Hamlin JL, Hsueh YP, Ianiri G, Jones S, Kodira CD, Kozubowski L, Lam W, Marra M, Mesner LD, Mieczkowski PA, Moyrand F, Nielsen K, Proux C, Rossignol T, Schein JE, Sun S, Wollschlaeger C, Wood IA, Zeng Q, Neuv?glise C, Newlon CS, Perfect JR, Lodge JK, Idnurm A, Stajich JE, Kronstad JW, Sanyal K, Heitman J, Fraser JA, Cuomo CA, Dietrich FS
PLoS genetics10(4)e1004261(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CNAG_05298 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNAG_05298 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr135717	XM_012193193.1 Latest version!	Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_05298), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCr135950	XM_012193446.1 Latest version!	Cryptococcus neoformans var. grubii H99 hypothetical protein, variant (CNAG_05298), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNAG_05298 ( XM_012193193.1 ) cDNA ORF clone, Cryptococcus neoformans var. grubii H99 -> XP_012048583.1 Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_05298), mRNA.

Price & Availability↑

CloneID	OCr135717
Clone ID Related Accession (Same CDS sequence)	XM_012193193.1
Accession Version	XM_012193193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Cryptococcus neoformans var. grubii H99
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026748).

ATGGATGAGC CGGCTCTGGC CGTAAGCACG GACACTTGCG AGCCCCATCA CTTTGCTCTC 
AATAAAACTC ACAACATGCC CTACTCGTTC GACGCCACCA CCGAGGGCAG ACCCAACCTC 
GCGAAACGAA ATACGGCTCC TTCTTCTGTG ACTCTTCTTC AGCTTCCGTC CCTTGAAGCG 
CTCCCCACTC CCCTCAAGGA TCGCGCACGC CGTGCCGCTT CTCAATCACA TCTACCTAGC 
CTGGAGGATT TTAGCACCAA ACCGCAATCT GTTTTCCAAA AGCTAAAGTC GAAACGGCCA 
CAGTCATCTT TGACAGCCAT GCCGCCTCTT CTCAAATCGC GTCGTCCCTC TTCACCCAAG 
GAACAAGCGT CTTTCTTTCC CTTTGATCTC CCTGCCATGG TTACAACGGC ATTCGAGCTT 
CGTCCCAAGG CGACTCGCAC CAACACTTCT TGCTCCACCA AGAATGAACC CGTGTCTTCC 
TCACTTCTTC CAGGTGAATC CTTCATTCCC TTCTCTACAC CATCAAACAC ATATTCCAAG 
TCCTCACTCG CTGCTATGCC GAGTATCCAA CACGTTATGG ACGCGTACGA CATTACCCAT 
TTGAAGGACC TTATGCCACA AGAGAAACAA CGGGAACGAG AGAGGAAAGA GCAAAGGAAG 
AAAAGGCGGG AAACGATCAA GCTGCCTATG ATGAAGCCTC CTCCCAGTCG ATCGAACTAC 
GGTAGCAAGA GAGAAGAGCT TGAGACTCTT GATGGTGATG ATTGGGAATA TACCTTTCCC 
CGCGACCTTT CCGCTTCATT TACCCAACCT CCGACTCCAA GCGTGCCCAC CCATTCTCAC 
AACCATGATG GTCAACGAAA TTATTCCATT CCTGTTGCCC TAGACTATAC CATACCCATT 
CCTCCTTCCC GCTGCGACTC TCGATCTCGA GACATTACAC GTCGCAACTT TGGCTCATTC 
GAGCAAGATA TTGGCTCGTT CGGTACGAAC AAGTCACATG ATATATGGCT ACAAAATCAA 
GTCGATACAC CAGAGGAGGT CGAGAGGGGC GCAGTGAGAG AGTTGGAGCA AATGAGTGGG 
AGGCTAATGG CTGATGGTTG GGGTTATGGT AATGGAAGTG CAAGTTGCCG TGCAAGTGGT 
CGAGCTAATC GTGCTGCTCA GCCAGAATTA TCTGCTACCC ATTCACCGCT TTCACCTGAA 
GCCTTAACGG TCCCCATCCT TACTACAGAC CCCCCTACTC CTCAACCGCG AAGACGCATG 
AGCGAACACG ACCAAATCGA TAAACTTTGT CAGCGCGAAC GTTCCCACAC TCAAGGGTAC 
AATGGTAGGA AAGAAACTAA ATGGACGGCT TCAAATCTTT ATCCCACTAC TCATCCTGCA 
GCCCACACTA TCGCCAGTCT TCAGCCTTTG CAACAGCGAA TCACTAGTTC AAATACACAT 
GACTGGGAGC AGGACAAGCT TGCGTCAATC AGTGGTCAGG GTGGGAAGAA AGGACAGAAC 
GGGGAGTCGC AGATGGATAG CACGGTGGTG AACGGTTTGA GTTTGGGCAT GTTGAAGTCC 
AAGAGAGCGA GTACGGGAGG GAAAAAGAGA CGGGATAGTG ATATACTGCT GCCAAAGAGG 
CAAGACTCTG GTTCATCCGC GCAATCTCAG CCCATCCAAA CACGACCTCT CCCGCCTATC 
CCCACCCAGC CGAATTCGCC CGGGACGTCT CCCATCTTCA AACACCGTAA AAGACCTTCC 
ACCGCCCCCC TCCCAGCCCT CGGATACTTC CCATCGCCCC GATCAACCGA TTCCCACTCT 
CATTTCAGCT CCAATTCCAA CCCTATCACC CCCGAAAAAC CCCGCAGACT CGTACCTCGC 
GACGAATCCC GCCTCTCCCT CTCCCCTTCT TCCTCAACTC ATACTCGCGA CGAAAGTTTC 
TCGCACGACA GAGATAGAGA AAGAGAAAAA GCCCGACAAA ACCTCTCTGC CATGGGTTTC 
TTGGCACTGG ATGAGATGTA CTTGCGACAG TCACTTGCGC TAGCTCAGCA GCCGCCCACG 
CCACCTATGC CGAGTAAGAT TGCATCCGGA GCGATGGGGG CTGCAAAGGG GATGGTGAAA 
GGGTTGAAAG CGAGGGTGAG CAATGCTCCA AGCATGGTAT CGCAAAGCGG GTATGTGACA 
ACTCCTCCGA CGCAGAAGAT CAAGGGACAA AAACGGAAGA GCATTTCTCA AGGGAAAGGG 
AAAGGGGAAG GGGTAAGAGA GGAAGGGAAT AGTATGGACT TTTCGGTGAA TGAGAATGAC 
GGTTGGAGTA GACCTACGTC AGCAGAAGCA ATGTACATTA TGCCACCCAC AGAGAGGAGT 
AAGATTGGGT CGCACGAGGT CGACAAGAAA CGCGGCTCCA GGAGGCGCAA AAGTCTGCAT 
GCGGAGAAGA CAAAGCAGAA CGGAGAAGGA GTTGAAGATG AATGTGGAGG AAAGTCTGGA 
AGTGAGAGCT CGGTACCGGG TAGTGCAGAC GAGACGTCGG TAGGCGTGAT CTCGCTTTTG 
GGCCATGGGG AAGAAGGTCT TAAAGAGGAT CCATGGTGCA CCGCAAAGGA GTGCGACTCG 
GCGAGACATC TTTCCCTCGA ACTTGCTCGC GAATGTCAAG CTCGAGCGAT GCTTACCTCC 
CTTCCTACTA CCGGCACTAC AACGCATACC AAAGCGAGAC CACCTCCTAT CGACGTCTCA 
TCTCCGACAA TCAACGGTTG GGAAAGGGAA GAAATTGAAA AAGGAGGTCT TACACCCAGT 
CTCTTACCCC CTCCAAGGCC TAGGAGAATG ACTGCTTCGG GCCAAGGGAG TGCACAAAGC 
ACCGAAAGCT CGAAAGGGAG ATCGCTGCCC CTCTTGGGTG GTATAGTAGA CGAAAGAAGT 
ACATTCTTCA ATCTCTCTAG ACCTTCACCG CCAATTACCC CTGATCCTTC GTACTTACCT 
TTGGTCCAGA CGCCCGCCCA GGCGATCGCG CAAAACTACC TTTCCTTCAT ACCTGCTAAC 
AATATCCACA ACTCCCCACA TGATATCCCC CGTCGTCCGA GCGACCCTGT CTCGGAACAC 
CGCCAACACC CACCCCAATG CCAAGGTCAA GCTCACCCTT TCTTACCTTA TCCAACCCCA 
ATCACTCCCA AACCCAGACC GCTTACGTCC AACTCTGTTC ATCCCTCATT CACCTCGCTC 
TCTCTTTCAC CTTCATCTAT TTCCGTCGAA TCGGACTCGA CAGCAGATAA AGAGAGGGAG 
GAAAAGGAAC GCCGGAGAAA GCTGTTGATG AGTGCATGGC TTGCTAGTAA GCCTGTGAGA 
AGAAGTGAAC AAGAGCAGGC TGGAGGAACT GCGCCTGCTG TTTTGGGAGG AGGAGGGGCG 
GCAAGAGAGG TTAGCAAGGG AGGAGCAAGA CCAGGGACAA GTGCAGGTAC CGGAATGAAG 
AGGGATGAGG ATGCGGGGTT CGCATATAGG AGGGCGAGTT TAGATAATAG GAGGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012048583.1
CDS	348..3827
Translation	MDEPALAVSTDTCEPHHFALNKTHNMPYSFDATTEGRPNLAKRNTAPSSVTLLQLPSLEALPTPLKDRARRAASQSHLPSLEDFSTKPQSVFQKLKSKRPQSSLTAMPPLLKSRRPSSPKEQASFFPFDLPAMVTTAFELRPKATRTNTSCSTKNEPVSSSLLPGESFIPFSTPSNTYSKSSLAAMPSIQHVMDAYDITHLKDLMPQEKQRERERKEQRKKRRETIKLPMMKPPPSRSNYGSKREELETLDGDDWEYTFPRDLSASFTQPPTPSVPTHSHNHDGQRNYSIPVALDYTIPIPPSRCDSRSRDITRRNFGSFEQDIGSFGTNKSHDIWLQNQVDTPEEVERGAVRELEQMSGRLMADGWGYGNGSASCRASGRANRAAQPELSATHSPLSPEALTVPILTTDPPTPQPRRRMSEHDQIDKLCQRERSHTQGYNGRKETKWTASNLYPTTHPAAHTIASLQPLQQRITSSNTHDWEQDKLASISGQGGKKGQNGESQMDSTVVNGLSLGMLKSKRASTGGKKRRDSDILLPKRQDSGSSAQSQPIQTRPLPPIPTQPNSPGTSPIFKHRKRPSTAPLPALGYFPSPRSTDSHSHFSSNSNPITPEKPRRLVPRDESRLSLSPSSSTHTRDESFSHDRDREREKARQNLSAMGFLALDEMYLRQSLALAQQPPTPPMPSKIASGAMGAAKGMVKGLKARVSNAPSMVSQSGYVTTPPTQKIKGQKRKSISQGKGKGEGVREEGNSMDFSVNENDGWSRPTSAEAMYIMPPTERSKIGSHEVDKKRGSRRRKSLHAEKTKQNGEGVEDECGGKSGSESSVPGSADETSVGVISLLGHGEEGLKEDPWCTAKECDSARHLSLELARECQARAMLTSLPTTGTTTHTKARPPPIDVSSPTINGWEREEIEKGGLTPSLLPPPRPRRMTASGQGSAQSTESSKGRSLPLLGGIVDERSTFFNLSRPSPPITPDPSYLPLVQTPAQAIAQNYLSFIPANNIHNSPHDIPRRPSDPVSEHRQHPPQCQGQAHPFLPYPTPITPKPRPLTSNSVHPSFTSLSLSPSSISVESDSTADKEREEKERRRKLLMSAWLASKPVRRSEQEQAGGTAPAVLGGGGAAREVSKGGARPGTSAGTGMKRDEDAGFAYRRASLDNRRF

Target ORF information:

RefSeq Version	XM_012193193.1
Organism	Cryptococcus neoformans var. grubii H99
Definition	Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_05298), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012193193.1

ORF Insert Sequence:

ATGGATGAGC CGGCTCTGGC CGTAAGCACG GACACTTGCG AGCCCCATCA CTTTGCTCTC 
AATAAAACTC ACAACATGCC CTACTCGTTC GACGCCACCA CCGAGGGCAG ACCCAACCTC 
GCGAAACGAA ATACGGCTCC TTCTTCTGTG ACTCTTCTTC AGCTTCCGTC CCTTGAAGCG 
CTCCCCACTC CCCTCAAGGA TCGCGCACGC CGTGCCGCTT CTCAATCACA TCTACCTAGC 
CTGGAGGATT TTAGCACCAA ACCGCAATCT GTTTTCCAAA AGCTAAAGTC GAAACGGCCA 
CAGTCATCTT TGACAGCCAT GCCGCCTCTT CTCAAATCGC GTCGTCCCTC TTCACCCAAG 
GAACAAGCGT CTTTCTTTCC CTTTGATCTC CCTGCCATGG TTACAACGGC ATTCGAGCTT 
CGTCCCAAGG CGACTCGCAC CAACACTTCT TGCTCCACCA AGAATGAACC CGTGTCTTCC 
TCACTTCTTC CAGGTGAATC CTTCATTCCC TTCTCTACAC CATCAAACAC ATATTCCAAG 
TCCTCACTCG CTGCTATGCC GAGTATCCAA CACGTTATGG ACGCGTACGA CATTACCCAT 
TTGAAGGACC TTATGCCACA AGAGAAACAA CGGGAACGAG AGAGGAAAGA GCAAAGGAAG 
AAAAGGCGGG AAACGATCAA GCTGCCTATG ATGAAGCCTC CTCCCAGTCG ATCGAACTAC 
GGTAGCAAGA GAGAAGAGCT TGAGACTCTT GATGGTGATG ATTGGGAATA TACCTTTCCC 
CGCGACCTTT CCGCTTCATT TACCCAACCT CCGACTCCAA GCGTGCCCAC CCATTCTCAC 
AACCATGATG GTCAACGAAA TTATTCCATT CCTGTTGCCC TAGACTATAC CATACCCATT 
CCTCCTTCCC GCTGCGACTC TCGATCTCGA GACATTACAC GTCGCAACTT TGGCTCATTC 
GAGCAAGATA TTGGCTCGTT CGGTACGAAC AAGTCACATG ATATATGGCT ACAAAATCAA 
GTCGATACAC CAGAGGAGGT CGAGAGGGGC GCAGTGAGAG AGTTGGAGCA AATGAGTGGG 
AGGCTAATGG CTGATGGTTG GGGTTATGGT AATGGAAGTG CAAGTTGCCG TGCAAGTGGT 
CGAGCTAATC GTGCTGCTCA GCCAGAATTA TCTGCTACCC ATTCACCGCT TTCACCTGAA 
GCCTTAACGG TCCCCATCCT TACTACAGAC CCCCCTACTC CTCAACCGCG AAGACGCATG 
AGCGAACACG ACCAAATCGA TAAACTTTGT CAGCGCGAAC GTTCCCACAC TCAAGGGTAC 
AATGGTAGGA AAGAAACTAA ATGGACGGCT TCAAATCTTT ATCCCACTAC TCATCCTGCA 
GCCCACACTA TCGCCAGTCT TCAGCCTTTG CAACAGCGAA TCACTAGTTC AAATACACAT 
GACTGGGAGC AGGACAAGCT TGCGTCAATC AGTGGTCAGG GTGGGAAGAA AGGACAGAAC 
GGGGAGTCGC AGATGGATAG CACGGTGGTG AACGGTTTGA GTTTGGGCAT GTTGAAGTCC 
AAGAGAGCGA GTACGGGAGG GAAAAAGAGA CGGGATAGTG ATATACTGCT GCCAAAGAGG 
CAAGACTCTG GTTCATCCGC GCAATCTCAG CCCATCCAAA CACGACCTCT CCCGCCTATC 
CCCACCCAGC CGAATTCGCC CGGGACGTCT CCCATCTTCA AACACCGTAA AAGACCTTCC 
ACCGCCCCCC TCCCAGCCCT CGGATACTTC CCATCGCCCC GATCAACCGA TTCCCACTCT 
CATTTCAGCT CCAATTCCAA CCCTATCACC CCCGAAAAAC CCCGCAGACT CGTACCTCGC 
GACGAATCCC GCCTCTCCCT CTCCCCTTCT TCCTCAACTC ATACTCGCGA CGAAAGTTTC 
TCGCACGACA GAGATAGAGA AAGAGAAAAA GCCCGACAAA ACCTCTCTGC CATGGGTTTC 
TTGGCACTGG ATGAGATGTA CTTGCGACAG TCACTTGCGC TAGCTCAGCA GCCGCCCACG 
CCACCTATGC CGAGTAAGAT TGCATCCGGA GCGATGGGGG CTGCAAAGGG GATGGTGAAA 
GGGTTGAAAG CGAGGGTGAG CAATGCTCCA AGCATGGTAT CGCAAAGCGG GTATGTGACA 
ACTCCTCCGA CGCAGAAGAT CAAGGGACAA AAACGGAAGA GCATTTCTCA AGGGAAAGGG 
AAAGGGGAAG GGGTAAGAGA GGAAGGGAAT AGTATGGACT TTTCGGTGAA TGAGAATGAC 
GGTTGGAGTA GACCTACGTC AGCAGAAGCA ATGTACATTA TGCCACCCAC AGAGAGGAGT 
AAGATTGGGT CGCACGAGGT CGACAAGAAA CGCGGCTCCA GGAGGCGCAA AAGTCTGCAT 
GCGGAGAAGA CAAAGCAGAA CGGAGAAGGA GTTGAAGATG AATGTGGAGG AAAGTCTGGA 
AGTGAGAGCT CGGTACCGGG TAGTGCAGAC GAGACGTCGG TAGGCGTGAT CTCGCTTTTG 
GGCCATGGGG AAGAAGGTCT TAAAGAGGAT CCATGGTGCA CCGCAAAGGA GTGCGACTCG 
GCGAGACATC TTTCCCTCGA ACTTGCTCGC GAATGTCAAG CTCGAGCGAT GCTTACCTCC 
CTTCCTACTA CCGGCACTAC AACGCATACC AAAGCGAGAC CACCTCCTAT CGACGTCTCA 
TCTCCGACAA TCAACGGTTG GGAAAGGGAA GAAATTGAAA AAGGAGGTCT TACACCCAGT 
CTCTTACCCC CTCCAAGGCC TAGGAGAATG ACTGCTTCGG GCCAAGGGAG TGCACAAAGC 
ACCGAAAGCT CGAAAGGGAG ATCGCTGCCC CTCTTGGGTG GTATAGTAGA CGAAAGAAGT 
ACATTCTTCA ATCTCTCTAG ACCTTCACCG CCAATTACCC CTGATCCTTC GTACTTACCT 
TTGGTCCAGA CGCCCGCCCA GGCGATCGCG CAAAACTACC TTTCCTTCAT ACCTGCTAAC 
AATATCCACA ACTCCCCACA TGATATCCCC CGTCGTCCGA GCGACCCTGT CTCGGAACAC 
CGCCAACACC CACCCCAATG CCAAGGTCAA GCTCACCCTT TCTTACCTTA TCCAACCCCA 
ATCACTCCCA AACCCAGACC GCTTACGTCC AACTCTGTTC ATCCCTCATT CACCTCGCTC 
TCTCTTTCAC CTTCATCTAT TTCCGTCGAA TCGGACTCGA CAGCAGATAA AGAGAGGGAG 
GAAAAGGAAC GCCGGAGAAA GCTGTTGATG AGTGCATGGC TTGCTAGTAA GCCTGTGAGA 
AGAAGTGAAC AAGAGCAGGC TGGAGGAACT GCGCCTGCTG TTTTGGGAGG AGGAGGGGCG 
GCAAGAGAGG TTAGCAAGGG AGGAGCAAGA CCAGGGACAA GTGCAGGTAC CGGAATGAAG 
AGGGATGAGG ATGCGGGGTT CGCATATAGG AGGGCGAGTT TAGATAATAG GAGGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNAG_05298 ( XM_012193446.1 ) cDNA ORF clone, Cryptococcus neoformans var. grubii H99 -> XP_012048836.1 Cryptococcus neoformans var. grubii H99 hypothetical protein, variant (CNAG_05298), mRNA.

Price & Availability↑

CloneID	OCr135950
Clone ID Related Accession (Same CDS sequence)	XM_012193446.1
Accession Version	XM_012193446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Cryptococcus neoformans var. grubii H99
Product	hypothetical protein, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026748).

ATGCCCTACT CGTTCGACGC CACCACCGAG GGCAGACCCA ACCTCGCGAA ACGAAATACG 
GCTCCTTCTT CTGTGACTCT TCTTCAGCTT CCGTCCCTTG AAGCGCTCCC CACTCCCCTC 
AAGGATCGCG CACGCCGTGC CGCTTCTCAA TCACATCTAC CTAGCCTGGA GGATTTTAGC 
ACCAAACCGC AATCTGTTTT CCAAAAGCTA AAGTCGAAAC GGCCACAGTC ATCTTTGACA 
GCCATGCCGC CTCTTCTCAA ATCGCGTCGT CCCTCTTCAC CCAAGGAACA AGCGTCTTTC 
TTTCCCTTTG ATCTCCCTGC CATGGTTACA ACGGCATTCG AGCTTCGTCC CAAGGCGACT 
CGCACCAACA CTTCTTGCTC CACCAAGAAT GAACCCGTGT CTTCCTCACT TCTTCCAGGT 
GAATCCTTCA TTCCCTTCTC TACACCATCA AACACATATT CCAAGTCCTC ACTCGCTGCT 
ATGCCGAGTA TCCAACACGT TATGGACGCG TACGACATTA CCCATTTGAA GGACCTTATG 
CCACAAGAGA AACAACGGGA ACGAGAGAGG AAAGAGCAAA GGAAGAAAAG GCGGGAAACG 
ATCAAGCTGC CTATGATGAA GCCTCCTCCC AGTCGATCGA ACTACGGTAG CAAGAGAGAA 
GAGCTTGAGA CTCTTGATGG TGATGATTGG GAATATACCT TTCCCCGCGA CCTTTCCGCT 
TCATTTACCC AACCTCCGAC TCCAAGCGTG CCCACCCATT CTCACAACCA TGATGGTCAA 
CGAAATTATT CCATTCCTGT TGCCCTAGAC TATACCATAC CCATTCCTCC TTCCCGCTGC 
GACTCTCGAT CTCGAGACAT TACACGTCGC AACTTTGGCT CATTCGAGCA AGATATTGGC 
TCGTTCGGTA CGAACAAGTC ACATGATATA TGGCTACAAA ATCAAGTCGA TACACCAGAG 
GAGGTCGAGA GGGGCGCAGT GAGAGAGTTG GAGCAAATGA GTGGGAGGCT AATGGCTGAT 
GGTTGGGGTT ATGGTAATGG AAGTGCAAGT TGCCGTGCAA GTGGTCGAGC TAATCGTGCT 
GCTCAGCCAG AATTATCTGC TACCCATTCA CCGCTTTCAC CTGAAGCCTT AACGGTCCCC 
ATCCTTACTA CAGACCCCCC TACTCCTCAA CCGCGAAGAC GCATGAGCGA ACACGACCAA 
ATCGATAAAC TTTGTCAGCG CGAACGTTCC CACACTCAAG GGTACAATGG TAGGAAAGAA 
ACTAAATGGA CGGCTTCAAA TCTTTATCCC ACTACTCATC CTGCAGCCCA CACTATCGCC 
AGTCTTCAGC CTTTGCAACA GCGAATCACT AGTTCAAATA CACATGACTG GGAGCAGGAC 
AAGCTTGCGT CAATCAGTGG TCAGGGTGGG AAGAAAGGAC AGAACGGGGA GTCGCAGATG 
GATAGCACGG TGGTGAACGG TTTGAGTTTG GGCATGTTGA AGTCCAAGAG AGCGAGTACG 
GGAGGGAAAA AGAGACGGGA TAGTGATATA CTGCTGCCAA AGAGGCAAGA CTCTGGTTCA 
TCCGCGCAAT CTCAGCCCAT CCAAACACGA CCTCTCCCGC CTATCCCCAC CCAGCCGAAT 
TCGCCCGGGA CGTCTCCCAT CTTCAAACAC CGTAAAAGAC CTTCCACCGC CCCCCTCCCA 
GCCCTCGGAT ACTTCCCATC GCCCCGATCA ACCGATTCCC ACTCTCATTT CAGCTCCAAT 
TCCAACCCTA TCACCCCCGA AAAACCCCGC AGACTCGTAC CTCGCGACGA ATCCCGCCTC 
TCCCTCTCCC CTTCTTCCTC AACTCATACT CGCGACGAAA GTTTCTCGCA CGACAGAGAT 
AGAGAAAGAG AAAAAGCCCG ACAAAACCTC TCTGCCATGG GTTTCTTGGC ACTGGATGAG 
ATGTACTTGC GACAGTCACT TGCGCTAGCT CAGCAGCCGC CCACGCCACC TATGCCGAGT 
AAGATTGCAT CCGGAGCGAT GGGGGCTGCA AAGGGGATGG TGAAAGGGTT GAAAGCGAGG 
GTGAGCAATG CTCCAAGCAT GGTATCGCAA AGCGGGTATG TGACAACTCC TCCGACGCAG 
AAGATCAAGG GACAAAAACG GAAGAGCATT TCTCAAGGGA AAGGGAAAGG GGAAGGGGTA 
AGAGAGGAAG GGAATAGTAT GGACTTTTCG GTGAATGAGA ATGACGGTTG GAGTAGACCT 
ACGTCAGCAG AAGCAATGTA CATTATGCCA CCCACAGAGA GGAGTAAGAT TGGGTCGCAC 
GAGGTCGACA AGAAACGCGG CTCCAGGAGG CGCAAAAGTC TGCATGCGGA GAAGACAAAG 
CAGAACGGAG AAGGAGTTGA AGATGAATGT GGAGGAAAGT CTGGAAGTGA GAGCTCGGTA 
CCGGGTAGTG CAGACGAGAC GTCGGTAGGC GTGATCTCGC TTTTGGGCCA TGGGGAAGAA 
GGTCTTAAAG AGGATCCATG GTGCACCGCA AAGGAGTGCG ACTCGGCGAG ACATCTTTCC 
CTCGAACTTG CTCGCGAATG TCAAGCTCGA GCGATGCTTA CCTCCCTTCC TACTACCGGC 
ACTACAACGC ATACCAAAGC GAGACCACCT CCTATCGACG TCTCATCTCC GACAATCAAC 
GGTTGGGAAA GGGAAGAAAT TGAAAAAGGA GGTCTTACAC CCAGTCTCTT ACCCCCTCCA 
AGGCCTAGGA GAATGACTGC TTCGGGCCAA GGGAGTGCAC AAAGCACCGA AAGCTCGAAA 
GGGAGATCGC TGCCCCTCTT GGGTGGTATA GTAGACGAAA GAAGTACATT CTTCAATCTC 
TCTAGACCTT CACCGCCAAT TACCCCTGAT CCTTCGTACT TACCTTTGGT CCAGACGCCC 
GCCCAGGCGA TCGCGCAAAA CTACCTTTCC TTCATACCTG CTAACAATAT CCACAACTCC 
CCACATGATA TCCCCCGTCG TCCGAGCGAC CCTGTCTCGG AACACCGCCA ACACCCACCC 
CAATGCCAAG GTCAAGCTCA CCCTTTCTTA CCTTATCCAA CCCCAATCAC TCCCAAACCC 
AGACCGCTTA CGTCCAACTC TGTTCATCCC TCATTCACCT CGCTCTCTCT TTCACCTTCA 
TCTATTTCCG TCGAATCGGA CTCGACAGCA GATAAAGAGA GGGAGGAAAA GGAACGCCGG 
AGAAAGCTGT TGATGAGTGC ATGGCTTGCT AGTAAGCCTG TGAGAAGAAG TGAACAAGAG 
CAGGCTGGAG GAACTGCGCC TGCTGTTTTG GGAGGAGGAG GGGCGGCAAG AGAGGTTAGC 
AAGGGAGGAG CAAGACCAGG GACAAGTGCA GGTACCGGAA TGAAGAGGGA TGAGGATGCG 
GGGTTCGCAT ATAGGAGGGC GAGTTTAGAT AATAGGAGGT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012048836.1
CDS	668..4072
Translation	MPYSFDATTEGRPNLAKRNTAPSSVTLLQLPSLEALPTPLKDRARRAASQSHLPSLEDFSTKPQSVFQKLKSKRPQSSLTAMPPLLKSRRPSSPKEQASFFPFDLPAMVTTAFELRPKATRTNTSCSTKNEPVSSSLLPGESFIPFSTPSNTYSKSSLAAMPSIQHVMDAYDITHLKDLMPQEKQRERERKEQRKKRRETIKLPMMKPPPSRSNYGSKREELETLDGDDWEYTFPRDLSASFTQPPTPSVPTHSHNHDGQRNYSIPVALDYTIPIPPSRCDSRSRDITRRNFGSFEQDIGSFGTNKSHDIWLQNQVDTPEEVERGAVRELEQMSGRLMADGWGYGNGSASCRASGRANRAAQPELSATHSPLSPEALTVPILTTDPPTPQPRRRMSEHDQIDKLCQRERSHTQGYNGRKETKWTASNLYPTTHPAAHTIASLQPLQQRITSSNTHDWEQDKLASISGQGGKKGQNGESQMDSTVVNGLSLGMLKSKRASTGGKKRRDSDILLPKRQDSGSSAQSQPIQTRPLPPIPTQPNSPGTSPIFKHRKRPSTAPLPALGYFPSPRSTDSHSHFSSNSNPITPEKPRRLVPRDESRLSLSPSSSTHTRDESFSHDRDREREKARQNLSAMGFLALDEMYLRQSLALAQQPPTPPMPSKIASGAMGAAKGMVKGLKARVSNAPSMVSQSGYVTTPPTQKIKGQKRKSISQGKGKGEGVREEGNSMDFSVNENDGWSRPTSAEAMYIMPPTERSKIGSHEVDKKRGSRRRKSLHAEKTKQNGEGVEDECGGKSGSESSVPGSADETSVGVISLLGHGEEGLKEDPWCTAKECDSARHLSLELARECQARAMLTSLPTTGTTTHTKARPPPIDVSSPTINGWEREEIEKGGLTPSLLPPPRPRRMTASGQGSAQSTESSKGRSLPLLGGIVDERSTFFNLSRPSPPITPDPSYLPLVQTPAQAIAQNYLSFIPANNIHNSPHDIPRRPSDPVSEHRQHPPQCQGQAHPFLPYPTPITPKPRPLTSNSVHPSFTSLSLSPSSISVESDSTADKEREEKERRRKLLMSAWLASKPVRRSEQEQAGGTAPAVLGGGGAAREVSKGGARPGTSAGTGMKRDEDAGFAYRRASLDNRRF

Target ORF information:

RefSeq Version	XM_012193446.1
Organism	Cryptococcus neoformans var. grubii H99
Definition	Cryptococcus neoformans var. grubii H99 hypothetical protein, variant (CNAG_05298), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012193446.1

ORF Insert Sequence:

ATGCCCTACT CGTTCGACGC CACCACCGAG GGCAGACCCA ACCTCGCGAA ACGAAATACG 
GCTCCTTCTT CTGTGACTCT TCTTCAGCTT CCGTCCCTTG AAGCGCTCCC CACTCCCCTC 
AAGGATCGCG CACGCCGTGC CGCTTCTCAA TCACATCTAC CTAGCCTGGA GGATTTTAGC 
ACCAAACCGC AATCTGTTTT CCAAAAGCTA AAGTCGAAAC GGCCACAGTC ATCTTTGACA 
GCCATGCCGC CTCTTCTCAA ATCGCGTCGT CCCTCTTCAC CCAAGGAACA AGCGTCTTTC 
TTTCCCTTTG ATCTCCCTGC CATGGTTACA ACGGCATTCG AGCTTCGTCC CAAGGCGACT 
CGCACCAACA CTTCTTGCTC CACCAAGAAT GAACCCGTGT CTTCCTCACT TCTTCCAGGT 
GAATCCTTCA TTCCCTTCTC TACACCATCA AACACATATT CCAAGTCCTC ACTCGCTGCT 
ATGCCGAGTA TCCAACACGT TATGGACGCG TACGACATTA CCCATTTGAA GGACCTTATG 
CCACAAGAGA AACAACGGGA ACGAGAGAGG AAAGAGCAAA GGAAGAAAAG GCGGGAAACG 
ATCAAGCTGC CTATGATGAA GCCTCCTCCC AGTCGATCGA ACTACGGTAG CAAGAGAGAA 
GAGCTTGAGA CTCTTGATGG TGATGATTGG GAATATACCT TTCCCCGCGA CCTTTCCGCT 
TCATTTACCC AACCTCCGAC TCCAAGCGTG CCCACCCATT CTCACAACCA TGATGGTCAA 
CGAAATTATT CCATTCCTGT TGCCCTAGAC TATACCATAC CCATTCCTCC TTCCCGCTGC 
GACTCTCGAT CTCGAGACAT TACACGTCGC AACTTTGGCT CATTCGAGCA AGATATTGGC 
TCGTTCGGTA CGAACAAGTC ACATGATATA TGGCTACAAA ATCAAGTCGA TACACCAGAG 
GAGGTCGAGA GGGGCGCAGT GAGAGAGTTG GAGCAAATGA GTGGGAGGCT AATGGCTGAT 
GGTTGGGGTT ATGGTAATGG AAGTGCAAGT TGCCGTGCAA GTGGTCGAGC TAATCGTGCT 
GCTCAGCCAG AATTATCTGC TACCCATTCA CCGCTTTCAC CTGAAGCCTT AACGGTCCCC 
ATCCTTACTA CAGACCCCCC TACTCCTCAA CCGCGAAGAC GCATGAGCGA ACACGACCAA 
ATCGATAAAC TTTGTCAGCG CGAACGTTCC CACACTCAAG GGTACAATGG TAGGAAAGAA 
ACTAAATGGA CGGCTTCAAA TCTTTATCCC ACTACTCATC CTGCAGCCCA CACTATCGCC 
AGTCTTCAGC CTTTGCAACA GCGAATCACT AGTTCAAATA CACATGACTG GGAGCAGGAC 
AAGCTTGCGT CAATCAGTGG TCAGGGTGGG AAGAAAGGAC AGAACGGGGA GTCGCAGATG 
GATAGCACGG TGGTGAACGG TTTGAGTTTG GGCATGTTGA AGTCCAAGAG AGCGAGTACG 
GGAGGGAAAA AGAGACGGGA TAGTGATATA CTGCTGCCAA AGAGGCAAGA CTCTGGTTCA 
TCCGCGCAAT CTCAGCCCAT CCAAACACGA CCTCTCCCGC CTATCCCCAC CCAGCCGAAT 
TCGCCCGGGA CGTCTCCCAT CTTCAAACAC CGTAAAAGAC CTTCCACCGC CCCCCTCCCA 
GCCCTCGGAT ACTTCCCATC GCCCCGATCA ACCGATTCCC ACTCTCATTT CAGCTCCAAT 
TCCAACCCTA TCACCCCCGA AAAACCCCGC AGACTCGTAC CTCGCGACGA ATCCCGCCTC 
TCCCTCTCCC CTTCTTCCTC AACTCATACT CGCGACGAAA GTTTCTCGCA CGACAGAGAT 
AGAGAAAGAG AAAAAGCCCG ACAAAACCTC TCTGCCATGG GTTTCTTGGC ACTGGATGAG 
ATGTACTTGC GACAGTCACT TGCGCTAGCT CAGCAGCCGC CCACGCCACC TATGCCGAGT 
AAGATTGCAT CCGGAGCGAT GGGGGCTGCA AAGGGGATGG TGAAAGGGTT GAAAGCGAGG 
GTGAGCAATG CTCCAAGCAT GGTATCGCAA AGCGGGTATG TGACAACTCC TCCGACGCAG 
AAGATCAAGG GACAAAAACG GAAGAGCATT TCTCAAGGGA AAGGGAAAGG GGAAGGGGTA 
AGAGAGGAAG GGAATAGTAT GGACTTTTCG GTGAATGAGA ATGACGGTTG GAGTAGACCT 
ACGTCAGCAG AAGCAATGTA CATTATGCCA CCCACAGAGA GGAGTAAGAT TGGGTCGCAC 
GAGGTCGACA AGAAACGCGG CTCCAGGAGG CGCAAAAGTC TGCATGCGGA GAAGACAAAG 
CAGAACGGAG AAGGAGTTGA AGATGAATGT GGAGGAAAGT CTGGAAGTGA GAGCTCGGTA 
CCGGGTAGTG CAGACGAGAC GTCGGTAGGC GTGATCTCGC TTTTGGGCCA TGGGGAAGAA 
GGTCTTAAAG AGGATCCATG GTGCACCGCA AAGGAGTGCG ACTCGGCGAG ACATCTTTCC 
CTCGAACTTG CTCGCGAATG TCAAGCTCGA GCGATGCTTA CCTCCCTTCC TACTACCGGC 
ACTACAACGC ATACCAAAGC GAGACCACCT CCTATCGACG TCTCATCTCC GACAATCAAC 
GGTTGGGAAA GGGAAGAAAT TGAAAAAGGA GGTCTTACAC CCAGTCTCTT ACCCCCTCCA 
AGGCCTAGGA GAATGACTGC TTCGGGCCAA GGGAGTGCAC AAAGCACCGA AAGCTCGAAA 
GGGAGATCGC TGCCCCTCTT GGGTGGTATA GTAGACGAAA GAAGTACATT CTTCAATCTC 
TCTAGACCTT CACCGCCAAT TACCCCTGAT CCTTCGTACT TACCTTTGGT CCAGACGCCC 
GCCCAGGCGA TCGCGCAAAA CTACCTTTCC TTCATACCTG CTAACAATAT CCACAACTCC 
CCACATGATA TCCCCCGTCG TCCGAGCGAC CCTGTCTCGG AACACCGCCA ACACCCACCC 
CAATGCCAAG GTCAAGCTCA CCCTTTCTTA CCTTATCCAA CCCCAATCAC TCCCAAACCC 
AGACCGCTTA CGTCCAACTC TGTTCATCCC TCATTCACCT CGCTCTCTCT TTCACCTTCA 
TCTATTTCCG TCGAATCGGA CTCGACAGCA GATAAAGAGA GGGAGGAAAA GGAACGCCGG 
AGAAAGCTGT TGATGAGTGC ATGGCTTGCT AGTAAGCCTG TGAGAAGAAG TGAACAAGAG 
CAGGCTGGAG GAACTGCGCC TGCTGTTTTG GGAGGAGGAG GGGCGGCAAG AGAGGTTAGC 
AAGGGAGGAG CAAGACCAGG GACAAGTGCA GGTACCGGAA TGAAGAGGGA TGAGGATGCG 
GGGTTCGCAT ATAGGAGGGC GAGTTTAGAT AATAGGAGGT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation.
PLoS genetics10(4)e1004261(2014 Apr)
Janbon G,Ormerod KL,Paulet D,Byrnes EJ 3rd,Yadav V,Chatterjee G,Mullapudi N,Hon CC,Billmyre RB,Brunel F,Bahn YS,Chen W,Chen Y,Chow EW,Copp?e JY,Floyd-Averette A,Gaillardin C,Gerik KJ,Goldberg J,Gonzalez-Hilarion S,Gujja S,Hamlin JL,Hsueh YP,Ianiri G,Jones S,Kodira CD,Kozubowski L,Lam W,Marra M,Mesner LD,Mieczkowski PA,Moyrand F,Nielsen K,Proux C,Rossignol T,Schein JE,Sun S,Wollschlaeger C,Wood IA,Zeng Q,Neuv?glise C,Newlon CS,Perfect JR,Lodge JK,Idnurm A,Stajich JE,Kronstad JW,Sanyal K,Heitman J,Fraser JA,Cuomo CA,Dietrich FS

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CNAG_05298 cDNA ORF clone, Cryptococcus neoformans var. grubii H99

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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