CRB2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRB2
Entrez Gene ID	417104
Full Name	crumbs 2, cell polarity complex component
General protein information	Preferred Names crumbs 2, cell polarity complex component Names protein crumbs homolog 2 crumbs family member 2 crumbs homolog 2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004946010.3	XP_004946067.1	protein crumbs homolog 2 isoform X2
XM_004946010.2	XP_004946067.1	protein crumbs homolog 2 isoform X2
XM_015279573.1	XP_015135059.1	protein crumbs homolog 2 isoform X3
XM_004946009.2	XP_004946066.1	protein crumbs homolog 2 isoform X1
XM_004946009.3	XP_004946066.1	protein crumbs homolog 2 isoform X1
XM_004946015.2	XP_004946072.1	protein crumbs homolog 2 isoform X4
XM_015279573.2	XP_015135059.1	protein crumbs homolog 2 isoform X3

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	crb	NP_001247284.1
Caenorhabditis elegans (roundworm)	crb-1	NP_510822.1
Xenopus tropicalis (tropical clawed frog)	LOC100487355	XP_004918981.1
Gallus gallus (chicken)	CRB2	XP_004946067.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003873	XP_310433.5
Danio rerio (zebrafish)	crb2b	NP_001038627.1
Danio rerio (zebrafish)	crb2a	NP_001038764.1

Gene Ontology

The following CRB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa43516	XM_015279573.2 Latest version!	Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa16384	XM_004946009.3 Latest version!	Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OGa16385	XM_004946010.3 Latest version!	Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OGa16391	XM_004946015.2 Latest version!	Gallus gallus crumbs family member 2 (CRB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OGa16385	XM_004946010.2	Gallus gallus crumbs family member 2 (CRB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OGa16384	XM_004946009.2	Gallus gallus crumbs family member 2 (CRB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OGa43516	XM_015279573.1	Gallus gallus crumbs family member 2 (CRB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRB2 ( XM_015279573.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135059.1 Gallus gallus crumbs family member 2 (CRB2), transcript variant X3, mRNA.

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CloneID	OGa43516
Clone ID Related Accession (Same CDS sequence)	XM_015279573.1 , XM_015279573.2
Accession Version	XM_015279573.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein crumbs homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCCA TAGCCTACAT GGGCATTAAC TGTGAACTCC TCTATGATGC ATGCACTAAA 
CATGATTGCC CTGCCCATAT GATTTGCAAC AAAACTCCAG GACTTCCGGA ATATGAATGC 
ATCTGTATGC CTGGCTTTAC AGGTATTGAT TGTAGCATTA ATATTAATGA GTGTGAGAGC 
AACCCTTGTA AAGATCCTCG TTTTGAATGT GTAGATAGTG TAAGTGGATA CGCCTGTAAA 
TGCCAAACAG GACTGAATGG AGAAGGCTGC CAAAACTCTG TGTGCTCTAG CCACCTCTGC 
CTAAATAACG GGACGTGTGT CGAGGGTCCT GGGGACTATG CCTGCATCTG CCAACCTGGC 
TTCACCGGGG CCCACTGTAA AGACAACATT GACGAGTGCG CTTCCAACCC ATGCCAGAAT 
GGAGCCATCT GCCGAGACAG GGTGAATGAG TATAGCTGTT TCTGTGTGCC TGGGTTCCAA 
GGATACAACT GTGAAATAGA CATCAACGAG TGTGCATCCC GGCCCTGTAA AAACAATGGC 
ACCTGCCTGA ATGAGATGGA TCACTATCTG TGCAAGTGCA TCCCCGGCTA CACAGGCGTA 
AACTGTGAAG CTGAAATAGA TGAATGTGAT TCATACCCTT GTCAGAATGG GGCCTTGTGC 
AGTGATCATA TTGGGTTCTA CACCTGCACC TGTATGCCAG GTTACCAAGG AATCCAGTGT 
GAGGTGGACA TCAATGAATG TATAAGCCAG CCATGCCAGC ACAATGGAAC ATGTCATGAT 
CTTATTAACA GTTACCAATG TGACTGCAGT GACACAGGCT TTGAAGGGGA CCACTGTGAG 
TTGGATATCC TGGAGTGTGC CTCTGAGCCC TGTCTGAACA ATGCCACATG TGTTGAAGGA 
ATCAAGAACT ACAGCTGTGC CTGCTGGACA GGTTACACAG GGCAGCATTG TGAAGAGGAT 
GTGAACGAGT GTGCGACAGA TCCCTGTCAC AATGGAGGTG TATGCTTTGA GCGATCCAAC 
CAGTCTTATT ATGGATCACA ACCTGACTTC CCCAGCGATT TCAGCTACAG CCAAGCAGCT 
GGTTTCCTCT GTTGGTGCCA GCCAGGCTTT GCAGGGGAGA CCTGCTTTAC TAACATCAAT 
GAGTGCGAGT CTCAGCCATG TCAGAATGGA GGTCACTGCG TGGACCTTGT TGATGGCTTT 
CTTTGTCATT GTTTACCAGG TTATTCAGGT GTGGAATGTG CTGTTAACAT CAATGAGTGT 
GAGGAGGGTC CCTGCAAGAA TGGAGCTGTC TGTGAGGATG GCATTGCTGA CTATACCTGC 
CACTGTGCCC CTAGCCAGGA TGGCATTGTA TGGGGAGGGA AGAACTGCTC TGTCAAGCTC 
ACTGGGTGCC AGACACATGA CTGCCAAAAT GAGGCCTTGT GTATCCCGAC TTACCAAGCT 
GAGAGCCACG GCCACCTGTG CCAGTGCCAG CCTGGTTTCT ATGATGCCAC ATGCTCAACA 
CCAACAACAT TTTCCTTTGC TTCCAGAGGG TATCTCCTCA TTGAGCTGCC CGTGAACAAT 
CAGAGCAGGA GGGAGGTAGC AGGAGAGCAG CTTGCCAGTG TGTCCCTCCG GTTCCGAACC 
ACCTTGCCCA GCGCCATCCT TTTTTACCGA GGCCATGAAG CTGAATACTT ATTCCTGGAG 
CTCTTGGATG GCATTCTGCG TACAGAACTG AAGAGGCAGG ATGAGTACCT CCTGCTGCTG 
GAGGAGCTGA GAGTTGATGA TGGCCATTGG CATAAAGTTG AAGTTGTTCT GCAGAACACT 
GTGCAGCTCA AACTCTGGCA CGACTCCTGT GATGCAGGTG TCTGCATGCA GAGCTCTCCT 
ATCCCACACA ATGCTACTCC GATCCCACAT GCCTTCCTGA GTGTGTATAT TGGAGGTGCT 
CAGGATCCAC TGGCCAACAA CACACAAAGC CAGCAGGGCT TTGTTGGCTG CCTGGAAGAC 
TTGCAGGTGG ATGCTGAAGC TGTGCTCCCG GTGGCTCTCC CTGTGAGCGA GGTTTCCTCT 
GTGACACAGG GCTGTGACCG CACTGAGTGG TGCCTCTCTG AGCCCTGCTT TCATGGCGGG 
CTGTGTGTGG ACCTTTGGAC AACGTTCAGG TGTGACTGTT TGAGGCCGTA TGGAGGCCCT 
GCTTGCTCAT ATGAACACCC AGCAGTAACA TTTGGCCTGG AGAATTCTAC TAGCTTTTCC 
TCTTTCATCC TTTCTGATAG CCTTGGTGCA GACTTCAACA TCTCCTTTTT CATTCGTAGT 
CTGAAACCTA ACGGTTTACT GTTGCAAATC AGCAATGAAA CAGACCCCTG CCTCACTATA 
TACTTGAAGA ATGGCAAGCT AAAGATTGAG ATGTTATCTA CAGATACTGT GACATTTCCA 
GAAAATTTAG TTGATGGGAG AAGACATTTG GTAGCTCTGT CTTTCCAAGG AGGAATTGTT 
GGCGTTCACC AACCAGACAC ATATGTGGAG CTGGGACAAC TGGTGGGAAG GCCTCTTTTA 
GCTGGCTATG ACGTGTATAT TGGTGGACGT CCTGACCCAG ACAGCACTGA CCTGTGGGGA 
GGGTATTTTA AAGGCTGCTT GCAAGACATC CAACTGAACG GCCATCAAAT AGAGTTTTTT 
CAGGTTGAGA ACTACAGTCT GCCAGATGAA CTTAATAGGA CTCAAAATAT TAATTTGGTA 
AATGGATGCA TCTCTGATAA CACCTGCAAG TCTGAACCAT GTCAGAATGG TGGAAGGTGC 
ATTGTCACGT GGAATGATTT CCATTGCAGC TGTCCAGCAA ATTTCACTGG GAAGTTTTGT 
GAAGAGAGAG TCTGGTGTGA AAGTGACCCA TGTCCTGAAG CTACCACCTG TATAGATGTT 
CCAGCAGGAT ATGTGTGTCT GGCTAACGCA ACATTTAATA GTTCTGCTAC CATTGAATTT 
ACCACCAATA CATCAGTGAC TAGAACTCTG GGCAGCCTCC TCATGGATTT CAGAACCAGA 
GACGAAGACG CTGTTTTGCT TCGGGCTATG GAAGAAGTGG ACTCCCTCCA GATAGCTATT 
AAGAACTCCT CCTTGCTTGT TGACATCAGG AGTGGGAATA GTATTGAAGG TGTCAGCTTC 
CTGAGTCAGA ATGCTGTCAC CGATGGCGCC TGGCATACAA TCTCTCTGTC AATGGAGGAG 
CCTTCTGCAC TGTCTTCCAG ATGGGTGGCT CATTTGGATG GATCTATTAA TATGACTCTG 
CAGGGAAATG CTGGGAACCT GGACTTCTTA AAGAACAACG CACTGATTGT TCTAGCTGAA 
AACTTCACAG GCTGCCTAGG ACAAGTGAAG ATAGGAGGGA TCTACCTGCC ATTCACCTCC 
CATCTCTCAT ATCCTCAGCC AGAACAGTTC CAGCAGTCCA GCAGAAGAAC TATCCAGTTA 
GGCTGCACGG GGGCTGATGT TTGTGCTTCT AGCCCTTGCA GCAATGCTGG CACCTGCAGG 
GACTTGTTCA ACTCCTTCAG CTGTGCCTGC AGTGCTGGCT GGGAGGGGCT GCTGTGCGAG 
TCCAATACTG ATGACTGCCA GTCAAGCCCA TGTGTTCATG GAAACTGCAC CGATAGACTA 
GCAGATTTTC AGTGTGAATG CTTCCGGGGA TATATTGGGA AGAAGTGTGA CATCAATGTG 
GATGACTGTG TGCGGCACCA GTGCCAAAAT GGAGCTACCT GCATTGATGG GGTTTATGGC 
TACTCCTGCA AGTGCCCAGC ACAGTATTCA GGACCTCGCT GCGAATGGCC GTTCCCACCT 
GAGCAGTGTG GTAAGAACTT CACCTGTCTG AATGGTGGCA AGTGTACCAC TGAGACCTGG 
GGAGCCAACT GCACTTGCAA GCCAGGCTTC ACTGGAAGGA ATTGTCAAAT TAATATAAAC 
AAATGTGATC CAAACCCCTG CCAGAATGGA GGCACCTGTC AAGACTCTGA GAACAAATTC 
AAGTGTTTGT GCAGTGCCAG CTACACTGGA GAGCGCTGTG ACATTAACAA AGGGACTCCA 
GGTGCTTTCT TCCCCTCCCC ACTAATTGAA GTAGCTGTCC CTGTAGCCTG TGGCTCTTTA 
CTGCTGCTCA GTATTGGTCT CATATTCATG ATTCTCACTG CAAGGAAGAG GCGCCAATCA 
GAGGGAACCT ACAGCCCAAG TCAGCAAGAA GTGGCAGGAG CTCGGCTGGA GATGGACAGT 
GTCCTAAAGG TGCCACCTGA AGAGCGGTTA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135059.1
CDS	728..4963
Translation	MHPIAYMGINCELLYDACTKHDCPAHMICNKTPGLPEYECICMPGFTGIDCSININECESNPCKDPRFECVDSVSGYACKCQTGLNGEGCQNSVCSSHLCLNNGTCVEGPGDYACICQPGFTGAHCKDNIDECASNPCQNGAICRDRVNEYSCFCVPGFQGYNCEIDINECASRPCKNNGTCLNEMDHYLCKCIPGYTGVNCEAEIDECDSYPCQNGALCSDHIGFYTCTCMPGYQGIQCEVDINECISQPCQHNGTCHDLINSYQCDCSDTGFEGDHCELDILECASEPCLNNATCVEGIKNYSCACWTGYTGQHCEEDVNECATDPCHNGGVCFERSNQSYYGSQPDFPSDFSYSQAAGFLCWCQPGFAGETCFTNINECESQPCQNGGHCVDLVDGFLCHCLPGYSGVECAVNINECEEGPCKNGAVCEDGIADYTCHCAPSQDGIVWGGKNCSVKLTGCQTHDCQNEALCIPTYQAESHGHLCQCQPGFYDATCSTPTTFSFASRGYLLIELPVNNQSRREVAGEQLASVSLRFRTTLPSAILFYRGHEAEYLFLELLDGILRTELKRQDEYLLLLEELRVDDGHWHKVEVVLQNTVQLKLWHDSCDAGVCMQSSPIPHNATPIPHAFLSVYIGGAQDPLANNTQSQQGFVGCLEDLQVDAEAVLPVALPVSEVSSVTQGCDRTEWCLSEPCFHGGLCVDLWTTFRCDCLRPYGGPACSYEHPAVTFGLENSTSFSSFILSDSLGADFNISFFIRSLKPNGLLLQISNETDPCLTIYLKNGKLKIEMLSTDTVTFPENLVDGRRHLVALSFQGGIVGVHQPDTYVELGQLVGRPLLAGYDVYIGGRPDPDSTDLWGGYFKGCLQDIQLNGHQIEFFQVENYSLPDELNRTQNINLVNGCISDNTCKSEPCQNGGRCIVTWNDFHCSCPANFTGKFCEERVWCESDPCPEATTCIDVPAGYVCLANATFNSSATIEFTTNTSVTRTLGSLLMDFRTRDEDAVLLRAMEEVDSLQIAIKNSSLLVDIRSGNSIEGVSFLSQNAVTDGAWHTISLSMEEPSALSSRWVAHLDGSINMTLQGNAGNLDFLKNNALIVLAENFTGCLGQVKIGGIYLPFTSHLSYPQPEQFQQSSRRTIQLGCTGADVCASSPCSNAGTCRDLFNSFSCACSAGWEGLLCESNTDDCQSSPCVHGNCTDRLADFQCECFRGYIGKKCDINVDDCVRHQCQNGATCIDGVYGYSCKCPAQYSGPRCEWPFPPEQCGKNFTCLNGGKCTTETWGANCTCKPGFTGRNCQININKCDPNPCQNGGTCQDSENKFKCLCSASYTGERCDINKGTPGAFFPSPLIEVAVPVACGSLLLLSIGLIFMILTARKRRQSEGTYSPSQQEVAGARLEMDSVLKVPPEERLI

Target ORF information:

RefSeq Version	XM_015279573.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus crumbs family member 2 (CRB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279573.1

ORF Insert Sequence:

ATGCACCCCA TAGCCTACAT GGGCATTAAC TGTGAACTCC TCTATGATGC ATGCACTAAA 
CATGATTGCC CTGCCCATAT GATTTGCAAC AAAACTCCAG GACTTCCGGA ATATGAATGC 
ATCTGTATGC CTGGCTTTAC AGGTATTGAT TGTAGCATTA ATATTAATGA GTGTGAGAGC 
AACCCTTGTA AAGATCCTCG TTTTGAATGT GTAGATAGTG TAAGTGGATA CGCCTGTAAA 
TGCCAAACAG GACTGAATGG AGAAGGCTGC CAAAACTCTG TGTGCTCTAG CCACCTCTGC 
CTAAATAACG GGACGTGTGT CGAGGGTCCT GGGGACTATG CCTGCATCTG CCAACCTGGC 
TTCACCGGGG CCCACTGTAA AGACAACATT GACGAGTGCG CTTCCAACCC ATGCCAGAAT 
GGAGCCATCT GCCGAGACAG GGTGAATGAG TATAGCTGTT TCTGTGTGCC TGGGTTCCAA 
GGATACAACT GTGAAATAGA CATCAACGAG TGTGCATCCC GGCCCTGTAA AAACAATGGC 
ACCTGCCTGA ATGAGATGGA TCACTATCTG TGCAAGTGCA TCCCCGGCTA CACAGGCGTA 
AACTGTGAAG CTGAAATAGA TGAATGTGAT TCATACCCTT GTCAGAATGG GGCCTTGTGC 
AGTGATCATA TTGGGTTCTA CACCTGCACC TGTATGCCAG GTTACCAAGG AATCCAGTGT 
GAGGTGGACA TCAATGAATG TATAAGCCAG CCATGCCAGC ACAATGGAAC ATGTCATGAT 
CTTATTAACA GTTACCAATG TGACTGCAGT GACACAGGCT TTGAAGGGGA CCACTGTGAG 
TTGGATATCC TGGAGTGTGC CTCTGAGCCC TGTCTGAACA ATGCCACATG TGTTGAAGGA 
ATCAAGAACT ACAGCTGTGC CTGCTGGACA GGTTACACAG GGCAGCATTG TGAAGAGGAT 
GTGAACGAGT GTGCGACAGA TCCCTGTCAC AATGGAGGTG TATGCTTTGA GCGATCCAAC 
CAGTCTTATT ATGGATCACA ACCTGACTTC CCCAGCGATT TCAGCTACAG CCAAGCAGCT 
GGTTTCCTCT GTTGGTGCCA GCCAGGCTTT GCAGGGGAGA CCTGCTTTAC TAACATCAAT 
GAGTGCGAGT CTCAGCCATG TCAGAATGGA GGTCACTGCG TGGACCTTGT TGATGGCTTT 
CTTTGTCATT GTTTACCAGG TTATTCAGGT GTGGAATGTG CTGTTAACAT CAATGAGTGT 
GAGGAGGGTC CCTGCAAGAA TGGAGCTGTC TGTGAGGATG GCATTGCTGA CTATACCTGC 
CACTGTGCCC CTAGCCAGGA TGGCATTGTA TGGGGAGGGA AGAACTGCTC TGTCAAGCTC 
ACTGGGTGCC AGACACATGA CTGCCAAAAT GAGGCCTTGT GTATCCCGAC TTACCAAGCT 
GAGAGCCACG GCCACCTGTG CCAGTGCCAG CCTGGTTTCT ATGATGCCAC ATGCTCAACA 
CCAACAACAT TTTCCTTTGC TTCCAGAGGG TATCTCCTCA TTGAGCTGCC CGTGAACAAT 
CAGAGCAGGA GGGAGGTAGC AGGAGAGCAG CTTGCCAGTG TGTCCCTCCG GTTCCGAACC 
ACCTTGCCCA GCGCCATCCT TTTTTACCGA GGCCATGAAG CTGAATACTT ATTCCTGGAG 
CTCTTGGATG GCATTCTGCG TACAGAACTG AAGAGGCAGG ATGAGTACCT CCTGCTGCTG 
GAGGAGCTGA GAGTTGATGA TGGCCATTGG CATAAAGTTG AAGTTGTTCT GCAGAACACT 
GTGCAGCTCA AACTCTGGCA CGACTCCTGT GATGCAGGTG TCTGCATGCA GAGCTCTCCT 
ATCCCACACA ATGCTACTCC GATCCCACAT GCCTTCCTGA GTGTGTATAT TGGAGGTGCT 
CAGGATCCAC TGGCCAACAA CACACAAAGC CAGCAGGGCT TTGTTGGCTG CCTGGAAGAC 
TTGCAGGTGG ATGCTGAAGC TGTGCTCCCG GTGGCTCTCC CTGTGAGCGA GGTTTCCTCT 
GTGACACAGG GCTGTGACCG CACTGAGTGG TGCCTCTCTG AGCCCTGCTT TCATGGCGGG 
CTGTGTGTGG ACCTTTGGAC AACGTTCAGG TGTGACTGTT TGAGGCCGTA TGGAGGCCCT 
GCTTGCTCAT ATGAACACCC AGCAGTAACA TTTGGCCTGG AGAATTCTAC TAGCTTTTCC 
TCTTTCATCC TTTCTGATAG CCTTGGTGCA GACTTCAACA TCTCCTTTTT CATTCGTAGT 
CTGAAACCTA ACGGTTTACT GTTGCAAATC AGCAATGAAA CAGACCCCTG CCTCACTATA 
TACTTGAAGA ATGGCAAGCT AAAGATTGAG ATGTTATCTA CAGATACTGT GACATTTCCA 
GAAAATTTAG TTGATGGGAG AAGACATTTG GTAGCTCTGT CTTTCCAAGG AGGAATTGTT 
GGCGTTCACC AACCAGACAC ATATGTGGAG CTGGGACAAC TGGTGGGAAG GCCTCTTTTA 
GCTGGCTATG ACGTGTATAT TGGTGGACGT CCTGACCCAG ACAGCACTGA CCTGTGGGGA 
GGGTATTTTA AAGGCTGCTT GCAAGACATC CAACTGAACG GCCATCAAAT AGAGTTTTTT 
CAGGTTGAGA ACTACAGTCT GCCAGATGAA CTTAATAGGA CTCAAAATAT TAATTTGGTA 
AATGGATGCA TCTCTGATAA CACCTGCAAG TCTGAACCAT GTCAGAATGG TGGAAGGTGC 
ATTGTCACGT GGAATGATTT CCATTGCAGC TGTCCAGCAA ATTTCACTGG GAAGTTTTGT 
GAAGAGAGAG TCTGGTGTGA AAGTGACCCA TGTCCTGAAG CTACCACCTG TATAGATGTT 
CCAGCAGGAT ATGTGTGTCT GGCTAACGCA ACATTTAATA GTTCTGCTAC CATTGAATTT 
ACCACCAATA CATCAGTGAC TAGAACTCTG GGCAGCCTCC TCATGGATTT CAGAACCAGA 
GACGAAGACG CTGTTTTGCT TCGGGCTATG GAAGAAGTGG ACTCCCTCCA GATAGCTATT 
AAGAACTCCT CCTTGCTTGT TGACATCAGG AGTGGGAATA GTATTGAAGG TGTCAGCTTC 
CTGAGTCAGA ATGCTGTCAC CGATGGCGCC TGGCATACAA TCTCTCTGTC AATGGAGGAG 
CCTTCTGCAC TGTCTTCCAG ATGGGTGGCT CATTTGGATG GATCTATTAA TATGACTCTG 
CAGGGAAATG CTGGGAACCT GGACTTCTTA AAGAACAACG CACTGATTGT TCTAGCTGAA 
AACTTCACAG GCTGCCTAGG ACAAGTGAAG ATAGGAGGGA TCTACCTGCC ATTCACCTCC 
CATCTCTCAT ATCCTCAGCC AGAACAGTTC CAGCAGTCCA GCAGAAGAAC TATCCAGTTA 
GGCTGCACGG GGGCTGATGT TTGTGCTTCT AGCCCTTGCA GCAATGCTGG CACCTGCAGG 
GACTTGTTCA ACTCCTTCAG CTGTGCCTGC AGTGCTGGCT GGGAGGGGCT GCTGTGCGAG 
TCCAATACTG ATGACTGCCA GTCAAGCCCA TGTGTTCATG GAAACTGCAC CGATAGACTA 
GCAGATTTTC AGTGTGAATG CTTCCGGGGA TATATTGGGA AGAAGTGTGA CATCAATGTG 
GATGACTGTG TGCGGCACCA GTGCCAAAAT GGAGCTACCT GCATTGATGG GGTTTATGGC 
TACTCCTGCA AGTGCCCAGC ACAGTATTCA GGACCTCGCT GCGAATGGCC GTTCCCACCT 
GAGCAGTGTG GTAAGAACTT CACCTGTCTG AATGGTGGCA AGTGTACCAC TGAGACCTGG 
GGAGCCAACT GCACTTGCAA GCCAGGCTTC ACTGGAAGGA ATTGTCAAAT TAATATAAAC 
AAATGTGATC CAAACCCCTG CCAGAATGGA GGCACCTGTC AAGACTCTGA GAACAAATTC 
AAGTGTTTGT GCAGTGCCAG CTACACTGGA GAGCGCTGTG ACATTAACAA AGGGACTCCA 
GGTGCTTTCT TCCCCTCCCC ACTAATTGAA GTAGCTGTCC CTGTAGCCTG TGGCTCTTTA 
CTGCTGCTCA GTATTGGTCT CATATTCATG ATTCTCACTG CAAGGAAGAG GCGCCAATCA 
GAGGGAACCT ACAGCCCAAG TCAGCAAGAA GTGGCAGGAG CTCGGCTGGA GATGGACAGT 
GTCCTAAAGG TGCCACCTGA AGAGCGGTTA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB2 ( XM_015279573.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135059.1 Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa43516
Clone ID Related Accession (Same CDS sequence)	XM_015279573.1 , XM_015279573.2
Accession Version	XM_015279573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein crumbs homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCCA TAGCCTACAT GGGCATTAAC TGTGAACTCC TCTATGATGC ATGCACTAAA 
CATGATTGCC CTGCCCATAT GATTTGCAAC AAAACTCCAG GACTTCCGGA ATATGAATGC 
ATCTGTATGC CTGGCTTTAC AGGTATTGAT TGTAGCATTA ATATTAATGA GTGTGAGAGC 
AACCCTTGTA AAGATCCTCG TTTTGAATGT GTAGATAGTG TAAGTGGATA CGCCTGTAAA 
TGCCAAACAG GACTGAATGG AGAAGGCTGC CAAAACTCTG TGTGCTCTAG CCACCTCTGC 
CTAAATAACG GGACGTGTGT CGAGGGTCCT GGGGACTATG CCTGCATCTG CCAACCTGGC 
TTCACCGGGG CCCACTGTAA AGACAACATT GACGAGTGCG CTTCCAACCC ATGCCAGAAT 
GGAGCCATCT GCCGAGACAG GGTGAATGAG TATAGCTGTT TCTGTGTGCC TGGGTTCCAA 
GGATACAACT GTGAAATAGA CATCAACGAG TGTGCATCCC GGCCCTGTAA AAACAATGGC 
ACCTGCCTGA ATGAGATGGA TCACTATCTG TGCAAGTGCA TCCCCGGCTA CACAGGCGTA 
AACTGTGAAG CTGAAATAGA TGAATGTGAT TCATACCCTT GTCAGAATGG GGCCTTGTGC 
AGTGATCATA TTGGGTTCTA CACCTGCACC TGTATGCCAG GTTACCAAGG AATCCAGTGT 
GAGGTGGACA TCAATGAATG TATAAGCCAG CCATGCCAGC ACAATGGAAC ATGTCATGAT 
CTTATTAACA GTTACCAATG TGACTGCAGT GACACAGGCT TTGAAGGGGA CCACTGTGAG 
TTGGATATCC TGGAGTGTGC CTCTGAGCCC TGTCTGAACA ATGCCACATG TGTTGAAGGA 
ATCAAGAACT ACAGCTGTGC CTGCTGGACA GGTTACACAG GGCAGCATTG TGAAGAGGAT 
GTGAACGAGT GTGCGACAGA TCCCTGTCAC AATGGAGGTG TATGCTTTGA GCGATCCAAC 
CAGTCTTATT ATGGATCACA ACCTGACTTC CCCAGCGATT TCAGCTACAG CCAAGCAGCT 
GGTTTCCTCT GTTGGTGCCA GCCAGGCTTT GCAGGGGAGA CCTGCTTTAC TAACATCAAT 
GAGTGCGAGT CTCAGCCATG TCAGAATGGA GGTCACTGCG TGGACCTTGT TGATGGCTTT 
CTTTGTCATT GTTTACCAGG TTATTCAGGT GTGGAATGTG CTGTTAACAT CAATGAGTGT 
GAGGAGGGTC CCTGCAAGAA TGGAGCTGTC TGTGAGGATG GCATTGCTGA CTATACCTGC 
CACTGTGCCC CTAGCCAGGA TGGCATTGTA TGGGGAGGGA AGAACTGCTC TGTCAAGCTC 
ACTGGGTGCC AGACACATGA CTGCCAAAAT GAGGCCTTGT GTATCCCGAC TTACCAAGCT 
GAGAGCCACG GCCACCTGTG CCAGTGCCAG CCTGGTTTCT ATGATGCCAC ATGCTCAACA 
CCAACAACAT TTTCCTTTGC TTCCAGAGGG TATCTCCTCA TTGAGCTGCC CGTGAACAAT 
CAGAGCAGGA GGGAGGTAGC AGGAGAGCAG CTTGCCAGTG TGTCCCTCCG GTTCCGAACC 
ACCTTGCCCA GCGCCATCCT TTTTTACCGA GGCCATGAAG CTGAATACTT ATTCCTGGAG 
CTCTTGGATG GCATTCTGCG TACAGAACTG AAGAGGCAGG ATGAGTACCT CCTGCTGCTG 
GAGGAGCTGA GAGTTGATGA TGGCCATTGG CATAAAGTTG AAGTTGTTCT GCAGAACACT 
GTGCAGCTCA AACTCTGGCA CGACTCCTGT GATGCAGGTG TCTGCATGCA GAGCTCTCCT 
ATCCCACACA ATGCTACTCC GATCCCACAT GCCTTCCTGA GTGTGTATAT TGGAGGTGCT 
CAGGATCCAC TGGCCAACAA CACACAAAGC CAGCAGGGCT TTGTTGGCTG CCTGGAAGAC 
TTGCAGGTGG ATGCTGAAGC TGTGCTCCCG GTGGCTCTCC CTGTGAGCGA GGTTTCCTCT 
GTGACACAGG GCTGTGACCG CACTGAGTGG TGCCTCTCTG AGCCCTGCTT TCATGGCGGG 
CTGTGTGTGG ACCTTTGGAC AACGTTCAGG TGTGACTGTT TGAGGCCGTA TGGAGGCCCT 
GCTTGCTCAT ATGAACACCC AGCAGTAACA TTTGGCCTGG AGAATTCTAC TAGCTTTTCC 
TCTTTCATCC TTTCTGATAG CCTTGGTGCA GACTTCAACA TCTCCTTTTT CATTCGTAGT 
CTGAAACCTA ACGGTTTACT GTTGCAAATC AGCAATGAAA CAGACCCCTG CCTCACTATA 
TACTTGAAGA ATGGCAAGCT AAAGATTGAG ATGTTATCTA CAGATACTGT GACATTTCCA 
GAAAATTTAG TTGATGGGAG AAGACATTTG GTAGCTCTGT CTTTCCAAGG AGGAATTGTT 
GGCGTTCACC AACCAGACAC ATATGTGGAG CTGGGACAAC TGGTGGGAAG GCCTCTTTTA 
GCTGGCTATG ACGTGTATAT TGGTGGACGT CCTGACCCAG ACAGCACTGA CCTGTGGGGA 
GGGTATTTTA AAGGCTGCTT GCAAGACATC CAACTGAACG GCCATCAAAT AGAGTTTTTT 
CAGGTTGAGA ACTACAGTCT GCCAGATGAA CTTAATAGGA CTCAAAATAT TAATTTGGTA 
AATGGATGCA TCTCTGATAA CACCTGCAAG TCTGAACCAT GTCAGAATGG TGGAAGGTGC 
ATTGTCACGT GGAATGATTT CCATTGCAGC TGTCCAGCAA ATTTCACTGG GAAGTTTTGT 
GAAGAGAGAG TCTGGTGTGA AAGTGACCCA TGTCCTGAAG CTACCACCTG TATAGATGTT 
CCAGCAGGAT ATGTGTGTCT GGCTAACGCA ACATTTAATA GTTCTGCTAC CATTGAATTT 
ACCACCAATA CATCAGTGAC TAGAACTCTG GGCAGCCTCC TCATGGATTT CAGAACCAGA 
GACGAAGACG CTGTTTTGCT TCGGGCTATG GAAGAAGTGG ACTCCCTCCA GATAGCTATT 
AAGAACTCCT CCTTGCTTGT TGACATCAGG AGTGGGAATA GTATTGAAGG TGTCAGCTTC 
CTGAGTCAGA ATGCTGTCAC CGATGGCGCC TGGCATACAA TCTCTCTGTC AATGGAGGAG 
CCTTCTGCAC TGTCTTCCAG ATGGGTGGCT CATTTGGATG GATCTATTAA TATGACTCTG 
CAGGGAAATG CTGGGAACCT GGACTTCTTA AAGAACAACG CACTGATTGT TCTAGCTGAA 
AACTTCACAG GCTGCCTAGG ACAAGTGAAG ATAGGAGGGA TCTACCTGCC ATTCACCTCC 
CATCTCTCAT ATCCTCAGCC AGAACAGTTC CAGCAGTCCA GCAGAAGAAC TATCCAGTTA 
GGCTGCACGG GGGCTGATGT TTGTGCTTCT AGCCCTTGCA GCAATGCTGG CACCTGCAGG 
GACTTGTTCA ACTCCTTCAG CTGTGCCTGC AGTGCTGGCT GGGAGGGGCT GCTGTGCGAG 
TCCAATACTG ATGACTGCCA GTCAAGCCCA TGTGTTCATG GAAACTGCAC CGATAGACTA 
GCAGATTTTC AGTGTGAATG CTTCCGGGGA TATATTGGGA AGAAGTGTGA CATCAATGTG 
GATGACTGTG TGCGGCACCA GTGCCAAAAT GGAGCTACCT GCATTGATGG GGTTTATGGC 
TACTCCTGCA AGTGCCCAGC ACAGTATTCA GGACCTCGCT GCGAATGGCC GTTCCCACCT 
GAGCAGTGTG GTAAGAACTT CACCTGTCTG AATGGTGGCA AGTGTACCAC TGAGACCTGG 
GGAGCCAACT GCACTTGCAA GCCAGGCTTC ACTGGAAGGA ATTGTCAAAT TAATATAAAC 
AAATGTGATC CAAACCCCTG CCAGAATGGA GGCACCTGTC AAGACTCTGA GAACAAATTC 
AAGTGTTTGT GCAGTGCCAG CTACACTGGA GAGCGCTGTG ACATTAACAA AGGGACTCCA 
GGTGCTTTCT TCCCCTCCCC ACTAATTGAA GTAGCTGTCC CTGTAGCCTG TGGCTCTTTA 
CTGCTGCTCA GTATTGGTCT CATATTCATG ATTCTCACTG CAAGGAAGAG GCGCCAATCA 
GAGGGAACCT ACAGCCCAAG TCAGCAAGAA GTGGCAGGAG CTCGGCTGGA GATGGACAGT 
GTCCTAAAGG TGCCACCTGA AGAGCGGTTA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135059.1
CDS	642..4877
Translation	MHPIAYMGINCELLYDACTKHDCPAHMICNKTPGLPEYECICMPGFTGIDCSININECESNPCKDPRFECVDSVSGYACKCQTGLNGEGCQNSVCSSHLCLNNGTCVEGPGDYACICQPGFTGAHCKDNIDECASNPCQNGAICRDRVNEYSCFCVPGFQGYNCEIDINECASRPCKNNGTCLNEMDHYLCKCIPGYTGVNCEAEIDECDSYPCQNGALCSDHIGFYTCTCMPGYQGIQCEVDINECISQPCQHNGTCHDLINSYQCDCSDTGFEGDHCELDILECASEPCLNNATCVEGIKNYSCACWTGYTGQHCEEDVNECATDPCHNGGVCFERSNQSYYGSQPDFPSDFSYSQAAGFLCWCQPGFAGETCFTNINECESQPCQNGGHCVDLVDGFLCHCLPGYSGVECAVNINECEEGPCKNGAVCEDGIADYTCHCAPSQDGIVWGGKNCSVKLTGCQTHDCQNEALCIPTYQAESHGHLCQCQPGFYDATCSTPTTFSFASRGYLLIELPVNNQSRREVAGEQLASVSLRFRTTLPSAILFYRGHEAEYLFLELLDGILRTELKRQDEYLLLLEELRVDDGHWHKVEVVLQNTVQLKLWHDSCDAGVCMQSSPIPHNATPIPHAFLSVYIGGAQDPLANNTQSQQGFVGCLEDLQVDAEAVLPVALPVSEVSSVTQGCDRTEWCLSEPCFHGGLCVDLWTTFRCDCLRPYGGPACSYEHPAVTFGLENSTSFSSFILSDSLGADFNISFFIRSLKPNGLLLQISNETDPCLTIYLKNGKLKIEMLSTDTVTFPENLVDGRRHLVALSFQGGIVGVHQPDTYVELGQLVGRPLLAGYDVYIGGRPDPDSTDLWGGYFKGCLQDIQLNGHQIEFFQVENYSLPDELNRTQNINLVNGCISDNTCKSEPCQNGGRCIVTWNDFHCSCPANFTGKFCEERVWCESDPCPEATTCIDVPAGYVCLANATFNSSATIEFTTNTSVTRTLGSLLMDFRTRDEDAVLLRAMEEVDSLQIAIKNSSLLVDIRSGNSIEGVSFLSQNAVTDGAWHTISLSMEEPSALSSRWVAHLDGSINMTLQGNAGNLDFLKNNALIVLAENFTGCLGQVKIGGIYLPFTSHLSYPQPEQFQQSSRRTIQLGCTGADVCASSPCSNAGTCRDLFNSFSCACSAGWEGLLCESNTDDCQSSPCVHGNCTDRLADFQCECFRGYIGKKCDINVDDCVRHQCQNGATCIDGVYGYSCKCPAQYSGPRCEWPFPPEQCGKNFTCLNGGKCTTETWGANCTCKPGFTGRNCQININKCDPNPCQNGGTCQDSENKFKCLCSASYTGERCDINKGTPGAFFPSPLIEVAVPVACGSLLLLSIGLIFMILTARKRRQSEGTYSPSQQEVAGARLEMDSVLKVPPEERLI

Target ORF information:

RefSeq Version	XM_015279573.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279573.2

ORF Insert Sequence:

ATGCACCCCA TAGCCTACAT GGGCATTAAC TGTGAACTCC TCTATGATGC ATGCACTAAA 
CATGATTGCC CTGCCCATAT GATTTGCAAC AAAACTCCAG GACTTCCGGA ATATGAATGC 
ATCTGTATGC CTGGCTTTAC AGGTATTGAT TGTAGCATTA ATATTAATGA GTGTGAGAGC 
AACCCTTGTA AAGATCCTCG TTTTGAATGT GTAGATAGTG TAAGTGGATA CGCCTGTAAA 
TGCCAAACAG GACTGAATGG AGAAGGCTGC CAAAACTCTG TGTGCTCTAG CCACCTCTGC 
CTAAATAACG GGACGTGTGT CGAGGGTCCT GGGGACTATG CCTGCATCTG CCAACCTGGC 
TTCACCGGGG CCCACTGTAA AGACAACATT GACGAGTGCG CTTCCAACCC ATGCCAGAAT 
GGAGCCATCT GCCGAGACAG GGTGAATGAG TATAGCTGTT TCTGTGTGCC TGGGTTCCAA 
GGATACAACT GTGAAATAGA CATCAACGAG TGTGCATCCC GGCCCTGTAA AAACAATGGC 
ACCTGCCTGA ATGAGATGGA TCACTATCTG TGCAAGTGCA TCCCCGGCTA CACAGGCGTA 
AACTGTGAAG CTGAAATAGA TGAATGTGAT TCATACCCTT GTCAGAATGG GGCCTTGTGC 
AGTGATCATA TTGGGTTCTA CACCTGCACC TGTATGCCAG GTTACCAAGG AATCCAGTGT 
GAGGTGGACA TCAATGAATG TATAAGCCAG CCATGCCAGC ACAATGGAAC ATGTCATGAT 
CTTATTAACA GTTACCAATG TGACTGCAGT GACACAGGCT TTGAAGGGGA CCACTGTGAG 
TTGGATATCC TGGAGTGTGC CTCTGAGCCC TGTCTGAACA ATGCCACATG TGTTGAAGGA 
ATCAAGAACT ACAGCTGTGC CTGCTGGACA GGTTACACAG GGCAGCATTG TGAAGAGGAT 
GTGAACGAGT GTGCGACAGA TCCCTGTCAC AATGGAGGTG TATGCTTTGA GCGATCCAAC 
CAGTCTTATT ATGGATCACA ACCTGACTTC CCCAGCGATT TCAGCTACAG CCAAGCAGCT 
GGTTTCCTCT GTTGGTGCCA GCCAGGCTTT GCAGGGGAGA CCTGCTTTAC TAACATCAAT 
GAGTGCGAGT CTCAGCCATG TCAGAATGGA GGTCACTGCG TGGACCTTGT TGATGGCTTT 
CTTTGTCATT GTTTACCAGG TTATTCAGGT GTGGAATGTG CTGTTAACAT CAATGAGTGT 
GAGGAGGGTC CCTGCAAGAA TGGAGCTGTC TGTGAGGATG GCATTGCTGA CTATACCTGC 
CACTGTGCCC CTAGCCAGGA TGGCATTGTA TGGGGAGGGA AGAACTGCTC TGTCAAGCTC 
ACTGGGTGCC AGACACATGA CTGCCAAAAT GAGGCCTTGT GTATCCCGAC TTACCAAGCT 
GAGAGCCACG GCCACCTGTG CCAGTGCCAG CCTGGTTTCT ATGATGCCAC ATGCTCAACA 
CCAACAACAT TTTCCTTTGC TTCCAGAGGG TATCTCCTCA TTGAGCTGCC CGTGAACAAT 
CAGAGCAGGA GGGAGGTAGC AGGAGAGCAG CTTGCCAGTG TGTCCCTCCG GTTCCGAACC 
ACCTTGCCCA GCGCCATCCT TTTTTACCGA GGCCATGAAG CTGAATACTT ATTCCTGGAG 
CTCTTGGATG GCATTCTGCG TACAGAACTG AAGAGGCAGG ATGAGTACCT CCTGCTGCTG 
GAGGAGCTGA GAGTTGATGA TGGCCATTGG CATAAAGTTG AAGTTGTTCT GCAGAACACT 
GTGCAGCTCA AACTCTGGCA CGACTCCTGT GATGCAGGTG TCTGCATGCA GAGCTCTCCT 
ATCCCACACA ATGCTACTCC GATCCCACAT GCCTTCCTGA GTGTGTATAT TGGAGGTGCT 
CAGGATCCAC TGGCCAACAA CACACAAAGC CAGCAGGGCT TTGTTGGCTG CCTGGAAGAC 
TTGCAGGTGG ATGCTGAAGC TGTGCTCCCG GTGGCTCTCC CTGTGAGCGA GGTTTCCTCT 
GTGACACAGG GCTGTGACCG CACTGAGTGG TGCCTCTCTG AGCCCTGCTT TCATGGCGGG 
CTGTGTGTGG ACCTTTGGAC AACGTTCAGG TGTGACTGTT TGAGGCCGTA TGGAGGCCCT 
GCTTGCTCAT ATGAACACCC AGCAGTAACA TTTGGCCTGG AGAATTCTAC TAGCTTTTCC 
TCTTTCATCC TTTCTGATAG CCTTGGTGCA GACTTCAACA TCTCCTTTTT CATTCGTAGT 
CTGAAACCTA ACGGTTTACT GTTGCAAATC AGCAATGAAA CAGACCCCTG CCTCACTATA 
TACTTGAAGA ATGGCAAGCT AAAGATTGAG ATGTTATCTA CAGATACTGT GACATTTCCA 
GAAAATTTAG TTGATGGGAG AAGACATTTG GTAGCTCTGT CTTTCCAAGG AGGAATTGTT 
GGCGTTCACC AACCAGACAC ATATGTGGAG CTGGGACAAC TGGTGGGAAG GCCTCTTTTA 
GCTGGCTATG ACGTGTATAT TGGTGGACGT CCTGACCCAG ACAGCACTGA CCTGTGGGGA 
GGGTATTTTA AAGGCTGCTT GCAAGACATC CAACTGAACG GCCATCAAAT AGAGTTTTTT 
CAGGTTGAGA ACTACAGTCT GCCAGATGAA CTTAATAGGA CTCAAAATAT TAATTTGGTA 
AATGGATGCA TCTCTGATAA CACCTGCAAG TCTGAACCAT GTCAGAATGG TGGAAGGTGC 
ATTGTCACGT GGAATGATTT CCATTGCAGC TGTCCAGCAA ATTTCACTGG GAAGTTTTGT 
GAAGAGAGAG TCTGGTGTGA AAGTGACCCA TGTCCTGAAG CTACCACCTG TATAGATGTT 
CCAGCAGGAT ATGTGTGTCT GGCTAACGCA ACATTTAATA GTTCTGCTAC CATTGAATTT 
ACCACCAATA CATCAGTGAC TAGAACTCTG GGCAGCCTCC TCATGGATTT CAGAACCAGA 
GACGAAGACG CTGTTTTGCT TCGGGCTATG GAAGAAGTGG ACTCCCTCCA GATAGCTATT 
AAGAACTCCT CCTTGCTTGT TGACATCAGG AGTGGGAATA GTATTGAAGG TGTCAGCTTC 
CTGAGTCAGA ATGCTGTCAC CGATGGCGCC TGGCATACAA TCTCTCTGTC AATGGAGGAG 
CCTTCTGCAC TGTCTTCCAG ATGGGTGGCT CATTTGGATG GATCTATTAA TATGACTCTG 
CAGGGAAATG CTGGGAACCT GGACTTCTTA AAGAACAACG CACTGATTGT TCTAGCTGAA 
AACTTCACAG GCTGCCTAGG ACAAGTGAAG ATAGGAGGGA TCTACCTGCC ATTCACCTCC 
CATCTCTCAT ATCCTCAGCC AGAACAGTTC CAGCAGTCCA GCAGAAGAAC TATCCAGTTA 
GGCTGCACGG GGGCTGATGT TTGTGCTTCT AGCCCTTGCA GCAATGCTGG CACCTGCAGG 
GACTTGTTCA ACTCCTTCAG CTGTGCCTGC AGTGCTGGCT GGGAGGGGCT GCTGTGCGAG 
TCCAATACTG ATGACTGCCA GTCAAGCCCA TGTGTTCATG GAAACTGCAC CGATAGACTA 
GCAGATTTTC AGTGTGAATG CTTCCGGGGA TATATTGGGA AGAAGTGTGA CATCAATGTG 
GATGACTGTG TGCGGCACCA GTGCCAAAAT GGAGCTACCT GCATTGATGG GGTTTATGGC 
TACTCCTGCA AGTGCCCAGC ACAGTATTCA GGACCTCGCT GCGAATGGCC GTTCCCACCT 
GAGCAGTGTG GTAAGAACTT CACCTGTCTG AATGGTGGCA AGTGTACCAC TGAGACCTGG 
GGAGCCAACT GCACTTGCAA GCCAGGCTTC ACTGGAAGGA ATTGTCAAAT TAATATAAAC 
AAATGTGATC CAAACCCCTG CCAGAATGGA GGCACCTGTC AAGACTCTGA GAACAAATTC 
AAGTGTTTGT GCAGTGCCAG CTACACTGGA GAGCGCTGTG ACATTAACAA AGGGACTCCA 
GGTGCTTTCT TCCCCTCCCC ACTAATTGAA GTAGCTGTCC CTGTAGCCTG TGGCTCTTTA 
CTGCTGCTCA GTATTGGTCT CATATTCATG ATTCTCACTG CAAGGAAGAG GCGCCAATCA 
GAGGGAACCT ACAGCCCAAG TCAGCAAGAA GTGGCAGGAG CTCGGCTGGA GATGGACAGT 
GTCCTAAAGG TGCCACCTGA AGAGCGGTTA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB2 ( XM_004946009.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004946066.1 Gallus gallus crumbs family member 2 (CRB2), transcript variant X1, mRNA.

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CloneID	OGa16384
Clone ID Related Accession (Same CDS sequence)	XM_004946009.2 , XM_004946009.3
Accession Version	XM_004946009.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein crumbs homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAATTGTC AAATTAATAT AAACAAATGT 
GATCCAAACC CCTGCCAGAA TGGAGGCACC TGTCAAGACT CTGAGAACAA ATTCAAGTGT 
TTGTGCAGTG CCAGCTACAC TGGAGAGCGC TGTGACATTA ACAAAGGGAC TCCAGGTGCT 
TTCTTCCCCT CCCCACTAAT TGAAGTAGCT GTCCCTGTAG CCTGTGGCTC TTTACTGCTG 
CTCAGTATTG GTCTCATATT CATGATTCTC ACTGCAAGGA AGAGGCGCCA ATCAGAGGGA 
ACCTACAGCC CAAGTCAGCA AGAAGTGGCA GGAGCTCGGC TGGAGATGGA CAGTGTCCTA 
AAGGTGCCAC CTGAAGAGCG GTTAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004946066.1
CDS	297..4706
Translation	MELKKTTSRVYNAALLLPLFLLFWGPSYSENDSSCSSSPCENGGSCKDLEAGYQCICPMHPIAYMGINCELLYDACTKHDCPAHMICNKTPGLPEYECICMPGFTGIDCSININECESNPCKDPRFECVDSVSGYACKCQTGLNGEGCQNSVCSSHLCLNNGTCVEGPGDYACICQPGFTGAHCKDNIDECASNPCQNGAICRDRVNEYSCFCVPGFQGYNCEIDINECASRPCKNNGTCLNEMDHYLCKCIPGYTGVNCEAEIDECDSYPCQNGALCSDHIGFYTCTCMPGYQGIQCEVDINECISQPCQHNGTCHDLINSYQCDCSDTGFEGDHCELDILECASEPCLNNATCVEGIKNYSCACWTGYTGQHCEEDVNECATDPCHNGGVCFERSNQSYYGSQPDFPSDFSYSQAAGFLCWCQPGFAGETCFTNINECESQPCQNGGHCVDLVDGFLCHCLPGYSGVECAVNINECEEGPCKNGAVCEDGIADYTCHCAPSQDGIVWGGKNCSVKLTGCQTHDCQNEALCIPTYQAESHGHLCQCQPGFYDATCSTPTTFSFASRGYLLIELPVNNQSRREVAGEQLASVSLRFRTTLPSAILFYRGHEAEYLFLELLDGILRTELKRQDEYLLLLEELRVDDGHWHKVEVVLQNTVQLKLWHDSCDAGVCMQSSPIPHNATPIPHAFLSVYIGGAQDPLANNTQSQQGFVGCLEDLQVDAEAVLPVALPVSEVSSVTQGCDRTEWCLSEPCFHGGLCVDLWTTFRCDCLRPYGGPACSYEHPAVTFGLENSTSFSSFILSDSLGADFNISFFIRSLKPNGLLLQISNETDPCLTIYLKNGKLKIEMLSTDTVTFPENLVDGRRHLVALSFQGGIVGVHQPDTYVELGQLVGRPLLAGYDVYIGGRPDPDSTDLWGGYFKGCLQDIQLNGHQIEFFQVENYSLPDELNRTQNINLVNGCISDNTCKSEPCQNGGRCIVTWNDFHCSCPANFTGKFCEERVWCESDPCPEATTCIDVPAGYVCLANATFNSSATIEFTTNTSVTRTLGSLLMDFRTRDEDAVLLRAMEEVDSLQIAIKNSSLLVDIRSGNSIEGVSFLSQNAVTDGAWHTISLSMEEPSALSSRWVAHLDGSINMTLQGNAGNLDFLKNNALIVLAENFTGCLGQVKIGGIYLPFTSHLSYPQPEQFQQSSRRTIQLGCTGADVCASSPCSNAGTCRDLFNSFSCACSAGWEGLLCESNTDDCQSSPCVHGNCTDRLADFQCECFRGYIGKKCDINVDDCVRHQCQNGATCIDGVYGYSCKCPAQYSGPRCEWPFPPEQCGKNFTCLNGGKCTTETWGANCTCKPGFTGRNCQININKCDPNPCQNGGTCQDSENKFKCLCSASYTGERCDINKGTPGAFFPSPLIEVAVPVACGSLLLLSIGLIFMILTARKRRQSEGTYSPSQQEVAGARLEMDSVLKVPPEERLI

Target ORF information:

RefSeq Version	XM_004946009.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus crumbs family member 2 (CRB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946009.2

ORF Insert Sequence:

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAATTGTC AAATTAATAT AAACAAATGT 
GATCCAAACC CCTGCCAGAA TGGAGGCACC TGTCAAGACT CTGAGAACAA ATTCAAGTGT 
TTGTGCAGTG CCAGCTACAC TGGAGAGCGC TGTGACATTA ACAAAGGGAC TCCAGGTGCT 
TTCTTCCCCT CCCCACTAAT TGAAGTAGCT GTCCCTGTAG CCTGTGGCTC TTTACTGCTG 
CTCAGTATTG GTCTCATATT CATGATTCTC ACTGCAAGGA AGAGGCGCCA ATCAGAGGGA 
ACCTACAGCC CAAGTCAGCA AGAAGTGGCA GGAGCTCGGC TGGAGATGGA CAGTGTCCTA 
AAGGTGCCAC CTGAAGAGCG GTTAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB2 ( XM_004946009.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004946066.1 Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X1, mRNA.

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CloneID	OGa16384
Clone ID Related Accession (Same CDS sequence)	XM_004946009.2 , XM_004946009.3
Accession Version	XM_004946009.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein crumbs homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004946009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAATTGTC AAATTAATAT AAACAAATGT 
GATCCAAACC CCTGCCAGAA TGGAGGCACC TGTCAAGACT CTGAGAACAA ATTCAAGTGT 
TTGTGCAGTG CCAGCTACAC TGGAGAGCGC TGTGACATTA ACAAAGGGAC TCCAGGTGCT 
TTCTTCCCCT CCCCACTAAT TGAAGTAGCT GTCCCTGTAG CCTGTGGCTC TTTACTGCTG 
CTCAGTATTG GTCTCATATT CATGATTCTC ACTGCAAGGA AGAGGCGCCA ATCAGAGGGA 
ACCTACAGCC CAAGTCAGCA AGAAGTGGCA GGAGCTCGGC TGGAGATGGA CAGTGTCCTA 
AAGGTGCCAC CTGAAGAGCG GTTAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004946066.1
CDS	1473..5882
Translation	MELKKTTSRVYNAALLLPLFLLFWGPSYSENDSSCSSSPCENGGSCKDLEAGYQCICPMHPIAYMGINCELLYDACTKHDCPAHMICNKTPGLPEYECICMPGFTGIDCSININECESNPCKDPRFECVDSVSGYACKCQTGLNGEGCQNSVCSSHLCLNNGTCVEGPGDYACICQPGFTGAHCKDNIDECASNPCQNGAICRDRVNEYSCFCVPGFQGYNCEIDINECASRPCKNNGTCLNEMDHYLCKCIPGYTGVNCEAEIDECDSYPCQNGALCSDHIGFYTCTCMPGYQGIQCEVDINECISQPCQHNGTCHDLINSYQCDCSDTGFEGDHCELDILECASEPCLNNATCVEGIKNYSCACWTGYTGQHCEEDVNECATDPCHNGGVCFERSNQSYYGSQPDFPSDFSYSQAAGFLCWCQPGFAGETCFTNINECESQPCQNGGHCVDLVDGFLCHCLPGYSGVECAVNINECEEGPCKNGAVCEDGIADYTCHCAPSQDGIVWGGKNCSVKLTGCQTHDCQNEALCIPTYQAESHGHLCQCQPGFYDATCSTPTTFSFASRGYLLIELPVNNQSRREVAGEQLASVSLRFRTTLPSAILFYRGHEAEYLFLELLDGILRTELKRQDEYLLLLEELRVDDGHWHKVEVVLQNTVQLKLWHDSCDAGVCMQSSPIPHNATPIPHAFLSVYIGGAQDPLANNTQSQQGFVGCLEDLQVDAEAVLPVALPVSEVSSVTQGCDRTEWCLSEPCFHGGLCVDLWTTFRCDCLRPYGGPACSYEHPAVTFGLENSTSFSSFILSDSLGADFNISFFIRSLKPNGLLLQISNETDPCLTIYLKNGKLKIEMLSTDTVTFPENLVDGRRHLVALSFQGGIVGVHQPDTYVELGQLVGRPLLAGYDVYIGGRPDPDSTDLWGGYFKGCLQDIQLNGHQIEFFQVENYSLPDELNRTQNINLVNGCISDNTCKSEPCQNGGRCIVTWNDFHCSCPANFTGKFCEERVWCESDPCPEATTCIDVPAGYVCLANATFNSSATIEFTTNTSVTRTLGSLLMDFRTRDEDAVLLRAMEEVDSLQIAIKNSSLLVDIRSGNSIEGVSFLSQNAVTDGAWHTISLSMEEPSALSSRWVAHLDGSINMTLQGNAGNLDFLKNNALIVLAENFTGCLGQVKIGGIYLPFTSHLSYPQPEQFQQSSRRTIQLGCTGADVCASSPCSNAGTCRDLFNSFSCACSAGWEGLLCESNTDDCQSSPCVHGNCTDRLADFQCECFRGYIGKKCDINVDDCVRHQCQNGATCIDGVYGYSCKCPAQYSGPRCEWPFPPEQCGKNFTCLNGGKCTTETWGANCTCKPGFTGRNCQININKCDPNPCQNGGTCQDSENKFKCLCSASYTGERCDINKGTPGAFFPSPLIEVAVPVACGSLLLLSIGLIFMILTARKRRQSEGTYSPSQQEVAGARLEMDSVLKVPPEERLI

Target ORF information:

RefSeq Version	XM_004946009.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946009.3

ORF Insert Sequence:

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAATTGTC AAATTAATAT AAACAAATGT 
GATCCAAACC CCTGCCAGAA TGGAGGCACC TGTCAAGACT CTGAGAACAA ATTCAAGTGT 
TTGTGCAGTG CCAGCTACAC TGGAGAGCGC TGTGACATTA ACAAAGGGAC TCCAGGTGCT 
TTCTTCCCCT CCCCACTAAT TGAAGTAGCT GTCCCTGTAG CCTGTGGCTC TTTACTGCTG 
CTCAGTATTG GTCTCATATT CATGATTCTC ACTGCAAGGA AGAGGCGCCA ATCAGAGGGA 
ACCTACAGCC CAAGTCAGCA AGAAGTGGCA GGAGCTCGGC TGGAGATGGA CAGTGTCCTA 
AAGGTGCCAC CTGAAGAGCG GTTAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB2 ( XM_004946010.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004946067.1 Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa16385
Clone ID Related Accession (Same CDS sequence)	XM_004946010.3 , XM_004946010.2
Accession Version	XM_004946010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein crumbs homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004946010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAATTGTC AAATTAATAT AAACAAATGT 
GATCCAAACC CCTGCCAGAA TGGAGGCACC TGTCAAGACT CTGAGAACAA ATTCAAGTGT 
TTGTGCAGTG CCAGCTACAC TGGAGAGCGC TGTGACATTA ACCAGCCTCT GTGGGCACAC 
CTTAGCAACT CCTCCGTTGT GGGAGCAGCT GGGGCTGCGG GGACTGCCCT TCTCCTTGCA 
GTTGGTGCAG CCACGGTGAT CGCCATGAAG AGGAGGCGAG CAACTCAGGG AAGATACAGT 
CCAAGCCGGC AAGAAAAGGA TGGTGCTCGA GTGGAATTGT GGAATGTAAT CAAGCTGCCA 
CCAACAGAGA GGCTGATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004946067.1
CDS	1475..5875
Translation	MELKKTTSRVYNAALLLPLFLLFWGPSYSENDSSCSSSPCENGGSCKDLEAGYQCICPMHPIAYMGINCELLYDACTKHDCPAHMICNKTPGLPEYECICMPGFTGIDCSININECESNPCKDPRFECVDSVSGYACKCQTGLNGEGCQNSVCSSHLCLNNGTCVEGPGDYACICQPGFTGAHCKDNIDECASNPCQNGAICRDRVNEYSCFCVPGFQGYNCEIDINECASRPCKNNGTCLNEMDHYLCKCIPGYTGVNCEAEIDECDSYPCQNGALCSDHIGFYTCTCMPGYQGIQCEVDINECISQPCQHNGTCHDLINSYQCDCSDTGFEGDHCELDILECASEPCLNNATCVEGIKNYSCACWTGYTGQHCEEDVNECATDPCHNGGVCFERSNQSYYGSQPDFPSDFSYSQAAGFLCWCQPGFAGETCFTNINECESQPCQNGGHCVDLVDGFLCHCLPGYSGVECAVNINECEEGPCKNGAVCEDGIADYTCHCAPSQDGIVWGGKNCSVKLTGCQTHDCQNEALCIPTYQAESHGHLCQCQPGFYDATCSTPTTFSFASRGYLLIELPVNNQSRREVAGEQLASVSLRFRTTLPSAILFYRGHEAEYLFLELLDGILRTELKRQDEYLLLLEELRVDDGHWHKVEVVLQNTVQLKLWHDSCDAGVCMQSSPIPHNATPIPHAFLSVYIGGAQDPLANNTQSQQGFVGCLEDLQVDAEAVLPVALPVSEVSSVTQGCDRTEWCLSEPCFHGGLCVDLWTTFRCDCLRPYGGPACSYEHPAVTFGLENSTSFSSFILSDSLGADFNISFFIRSLKPNGLLLQISNETDPCLTIYLKNGKLKIEMLSTDTVTFPENLVDGRRHLVALSFQGGIVGVHQPDTYVELGQLVGRPLLAGYDVYIGGRPDPDSTDLWGGYFKGCLQDIQLNGHQIEFFQVENYSLPDELNRTQNINLVNGCISDNTCKSEPCQNGGRCIVTWNDFHCSCPANFTGKFCEERVWCESDPCPEATTCIDVPAGYVCLANATFNSSATIEFTTNTSVTRTLGSLLMDFRTRDEDAVLLRAMEEVDSLQIAIKNSSLLVDIRSGNSIEGVSFLSQNAVTDGAWHTISLSMEEPSALSSRWVAHLDGSINMTLQGNAGNLDFLKNNALIVLAENFTGCLGQVKIGGIYLPFTSHLSYPQPEQFQQSSRRTIQLGCTGADVCASSPCSNAGTCRDLFNSFSCACSAGWEGLLCESNTDDCQSSPCVHGNCTDRLADFQCECFRGYIGKKCDINVDDCVRHQCQNGATCIDGVYGYSCKCPAQYSGPRCEWPFPPEQCGKNFTCLNGGKCTTETWGANCTCKPGFTGRNCQININKCDPNPCQNGGTCQDSENKFKCLCSASYTGERCDINQPLWAHLSNSSVVGAAGAAGTALLLAVGAATVIAMKRRRATQGRYSPSRQEKDGARVELWNVIKLPPTERLI

Target ORF information:

RefSeq Version	XM_004946010.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus crumbs 2, cell polarity complex component (CRB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946010.3

ORF Insert Sequence:

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAATTGTC AAATTAATAT AAACAAATGT 
GATCCAAACC CCTGCCAGAA TGGAGGCACC TGTCAAGACT CTGAGAACAA ATTCAAGTGT 
TTGTGCAGTG CCAGCTACAC TGGAGAGCGC TGTGACATTA ACCAGCCTCT GTGGGCACAC 
CTTAGCAACT CCTCCGTTGT GGGAGCAGCT GGGGCTGCGG GGACTGCCCT TCTCCTTGCA 
GTTGGTGCAG CCACGGTGAT CGCCATGAAG AGGAGGCGAG CAACTCAGGG AAGATACAGT 
CCAAGCCGGC AAGAAAAGGA TGGTGCTCGA GTGGAATTGT GGAATGTAAT CAAGCTGCCA 
CCAACAGAGA GGCTGATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB2 ( XM_004946010.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004946067.1 Gallus gallus crumbs family member 2 (CRB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa16385
Clone ID Related Accession (Same CDS sequence)	XM_004946010.3 , XM_004946010.2
Accession Version	XM_004946010.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein crumbs homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAATTGTC AAATTAATAT AAACAAATGT 
GATCCAAACC CCTGCCAGAA TGGAGGCACC TGTCAAGACT CTGAGAACAA ATTCAAGTGT 
TTGTGCAGTG CCAGCTACAC TGGAGAGCGC TGTGACATTA ACCAGCCTCT GTGGGCACAC 
CTTAGCAACT CCTCCGTTGT GGGAGCAGCT GGGGCTGCGG GGACTGCCCT TCTCCTTGCA 
GTTGGTGCAG CCACGGTGAT CGCCATGAAG AGGAGGCGAG CAACTCAGGG AAGATACAGT 
CCAAGCCGGC AAGAAAAGGA TGGTGCTCGA GTGGAATTGT GGAATGTAAT CAAGCTGCCA 
CCAACAGAGA GGCTGATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004946067.1
CDS	296..4696
Translation	MELKKTTSRVYNAALLLPLFLLFWGPSYSENDSSCSSSPCENGGSCKDLEAGYQCICPMHPIAYMGINCELLYDACTKHDCPAHMICNKTPGLPEYECICMPGFTGIDCSININECESNPCKDPRFECVDSVSGYACKCQTGLNGEGCQNSVCSSHLCLNNGTCVEGPGDYACICQPGFTGAHCKDNIDECASNPCQNGAICRDRVNEYSCFCVPGFQGYNCEIDINECASRPCKNNGTCLNEMDHYLCKCIPGYTGVNCEAEIDECDSYPCQNGALCSDHIGFYTCTCMPGYQGIQCEVDINECISQPCQHNGTCHDLINSYQCDCSDTGFEGDHCELDILECASEPCLNNATCVEGIKNYSCACWTGYTGQHCEEDVNECATDPCHNGGVCFERSNQSYYGSQPDFPSDFSYSQAAGFLCWCQPGFAGETCFTNINECESQPCQNGGHCVDLVDGFLCHCLPGYSGVECAVNINECEEGPCKNGAVCEDGIADYTCHCAPSQDGIVWGGKNCSVKLTGCQTHDCQNEALCIPTYQAESHGHLCQCQPGFYDATCSTPTTFSFASRGYLLIELPVNNQSRREVAGEQLASVSLRFRTTLPSAILFYRGHEAEYLFLELLDGILRTELKRQDEYLLLLEELRVDDGHWHKVEVVLQNTVQLKLWHDSCDAGVCMQSSPIPHNATPIPHAFLSVYIGGAQDPLANNTQSQQGFVGCLEDLQVDAEAVLPVALPVSEVSSVTQGCDRTEWCLSEPCFHGGLCVDLWTTFRCDCLRPYGGPACSYEHPAVTFGLENSTSFSSFILSDSLGADFNISFFIRSLKPNGLLLQISNETDPCLTIYLKNGKLKIEMLSTDTVTFPENLVDGRRHLVALSFQGGIVGVHQPDTYVELGQLVGRPLLAGYDVYIGGRPDPDSTDLWGGYFKGCLQDIQLNGHQIEFFQVENYSLPDELNRTQNINLVNGCISDNTCKSEPCQNGGRCIVTWNDFHCSCPANFTGKFCEERVWCESDPCPEATTCIDVPAGYVCLANATFNSSATIEFTTNTSVTRTLGSLLMDFRTRDEDAVLLRAMEEVDSLQIAIKNSSLLVDIRSGNSIEGVSFLSQNAVTDGAWHTISLSMEEPSALSSRWVAHLDGSINMTLQGNAGNLDFLKNNALIVLAENFTGCLGQVKIGGIYLPFTSHLSYPQPEQFQQSSRRTIQLGCTGADVCASSPCSNAGTCRDLFNSFSCACSAGWEGLLCESNTDDCQSSPCVHGNCTDRLADFQCECFRGYIGKKCDINVDDCVRHQCQNGATCIDGVYGYSCKCPAQYSGPRCEWPFPPEQCGKNFTCLNGGKCTTETWGANCTCKPGFTGRNCQININKCDPNPCQNGGTCQDSENKFKCLCSASYTGERCDINQPLWAHLSNSSVVGAAGAAGTALLLAVGAATVIAMKRRRATQGRYSPSRQEKDGARVELWNVIKLPPTERLI

Target ORF information:

RefSeq Version	XM_004946010.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus crumbs family member 2 (CRB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946010.2

ORF Insert Sequence:

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAATTGTC AAATTAATAT AAACAAATGT 
GATCCAAACC CCTGCCAGAA TGGAGGCACC TGTCAAGACT CTGAGAACAA ATTCAAGTGT 
TTGTGCAGTG CCAGCTACAC TGGAGAGCGC TGTGACATTA ACCAGCCTCT GTGGGCACAC 
CTTAGCAACT CCTCCGTTGT GGGAGCAGCT GGGGCTGCGG GGACTGCCCT TCTCCTTGCA 
GTTGGTGCAG CCACGGTGAT CGCCATGAAG AGGAGGCGAG CAACTCAGGG AAGATACAGT 
CCAAGCCGGC AAGAAAAGGA TGGTGCTCGA GTGGAATTGT GGAATGTAAT CAAGCTGCCA 
CCAACAGAGA GGCTGATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB2 ( XM_004946015.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004946072.1 Gallus gallus crumbs family member 2 (CRB2), transcript variant X4, mRNA.

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CloneID	OGa16391
Clone ID Related Accession (Same CDS sequence)	XM_004946015.2
Accession Version	XM_004946015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein crumbs homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAACTTTT CCTTTCCAGT TGTCAAATTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004946072.1
CDS	300..4385
Translation	MELKKTTSRVYNAALLLPLFLLFWGPSYSENDSSCSSSPCENGGSCKDLEAGYQCICPMHPIAYMGINCELLYDACTKHDCPAHMICNKTPGLPEYECICMPGFTGIDCSININECESNPCKDPRFECVDSVSGYACKCQTGLNGEGCQNSVCSSHLCLNNGTCVEGPGDYACICQPGFTGAHCKDNIDECASNPCQNGAICRDRVNEYSCFCVPGFQGYNCEIDINECASRPCKNNGTCLNEMDHYLCKCIPGYTGVNCEAEIDECDSYPCQNGALCSDHIGFYTCTCMPGYQGIQCEVDINECISQPCQHNGTCHDLINSYQCDCSDTGFEGDHCELDILECASEPCLNNATCVEGIKNYSCACWTGYTGQHCEEDVNECATDPCHNGGVCFERSNQSYYGSQPDFPSDFSYSQAAGFLCWCQPGFAGETCFTNINECESQPCQNGGHCVDLVDGFLCHCLPGYSGVECAVNINECEEGPCKNGAVCEDGIADYTCHCAPSQDGIVWGGKNCSVKLTGCQTHDCQNEALCIPTYQAESHGHLCQCQPGFYDATCSTPTTFSFASRGYLLIELPVNNQSRREVAGEQLASVSLRFRTTLPSAILFYRGHEAEYLFLELLDGILRTELKRQDEYLLLLEELRVDDGHWHKVEVVLQNTVQLKLWHDSCDAGVCMQSSPIPHNATPIPHAFLSVYIGGAQDPLANNTQSQQGFVGCLEDLQVDAEAVLPVALPVSEVSSVTQGCDRTEWCLSEPCFHGGLCVDLWTTFRCDCLRPYGGPACSYEHPAVTFGLENSTSFSSFILSDSLGADFNISFFIRSLKPNGLLLQISNETDPCLTIYLKNGKLKIEMLSTDTVTFPENLVDGRRHLVALSFQGGIVGVHQPDTYVELGQLVGRPLLAGYDVYIGGRPDPDSTDLWGGYFKGCLQDIQLNGHQIEFFQVENYSLPDELNRTQNINLVNGCISDNTCKSEPCQNGGRCIVTWNDFHCSCPANFTGKFCEERVWCESDPCPEATTCIDVPAGYVCLANATFNSSATIEFTTNTSVTRTLGSLLMDFRTRDEDAVLLRAMEEVDSLQIAIKNSSLLVDIRSGNSIEGVSFLSQNAVTDGAWHTISLSMEEPSALSSRWVAHLDGSINMTLQGNAGNLDFLKNNALIVLAENFTGCLGQVKIGGIYLPFTSHLSYPQPEQFQQSSRRTIQLGCTGADVCASSPCSNAGTCRDLFNSFSCACSAGWEGLLCESNTDDCQSSPCVHGNCTDRLADFQCECFRGYIGKKCDINVDDCVRHQCQNGATCIDGVYGYSCKCPAQYSGPRCEWPFPPEQCGKNFTCLNGGKCTTETWGANCTCKPGFTGRNFSFPVVKLI

Target ORF information:

RefSeq Version	XM_004946015.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus crumbs family member 2 (CRB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946015.2

ORF Insert Sequence:

ATGGAATTAA AGAAAACTAC ATCCAGGGTA TACAATGCTG CTCTCCTGCT GCCACTTTTC 
CTCTTATTTT GGGGACCTTC ATACTCTGAA AATGATAGCA GTTGTTCATC ATCGCCATGT 
GAAAATGGCG GGAGCTGTAA GGATTTGGAA GCGGGGTACC AGTGCATCTG CCCCATGCAC 
CCCATAGCCT ACATGGGCAT TAACTGTGAA CTCCTCTATG ATGCATGCAC TAAACATGAT 
TGCCCTGCCC ATATGATTTG CAACAAAACT CCAGGACTTC CGGAATATGA ATGCATCTGT 
ATGCCTGGCT TTACAGGTAT TGATTGTAGC ATTAATATTA ATGAGTGTGA GAGCAACCCT 
TGTAAAGATC CTCGTTTTGA ATGTGTAGAT AGTGTAAGTG GATACGCCTG TAAATGCCAA 
ACAGGACTGA ATGGAGAAGG CTGCCAAAAC TCTGTGTGCT CTAGCCACCT CTGCCTAAAT 
AACGGGACGT GTGTCGAGGG TCCTGGGGAC TATGCCTGCA TCTGCCAACC TGGCTTCACC 
GGGGCCCACT GTAAAGACAA CATTGACGAG TGCGCTTCCA ACCCATGCCA GAATGGAGCC 
ATCTGCCGAG ACAGGGTGAA TGAGTATAGC TGTTTCTGTG TGCCTGGGTT CCAAGGATAC 
AACTGTGAAA TAGACATCAA CGAGTGTGCA TCCCGGCCCT GTAAAAACAA TGGCACCTGC 
CTGAATGAGA TGGATCACTA TCTGTGCAAG TGCATCCCCG GCTACACAGG CGTAAACTGT 
GAAGCTGAAA TAGATGAATG TGATTCATAC CCTTGTCAGA ATGGGGCCTT GTGCAGTGAT 
CATATTGGGT TCTACACCTG CACCTGTATG CCAGGTTACC AAGGAATCCA GTGTGAGGTG 
GACATCAATG AATGTATAAG CCAGCCATGC CAGCACAATG GAACATGTCA TGATCTTATT 
AACAGTTACC AATGTGACTG CAGTGACACA GGCTTTGAAG GGGACCACTG TGAGTTGGAT 
ATCCTGGAGT GTGCCTCTGA GCCCTGTCTG AACAATGCCA CATGTGTTGA AGGAATCAAG 
AACTACAGCT GTGCCTGCTG GACAGGTTAC ACAGGGCAGC ATTGTGAAGA GGATGTGAAC 
GAGTGTGCGA CAGATCCCTG TCACAATGGA GGTGTATGCT TTGAGCGATC CAACCAGTCT 
TATTATGGAT CACAACCTGA CTTCCCCAGC GATTTCAGCT ACAGCCAAGC AGCTGGTTTC 
CTCTGTTGGT GCCAGCCAGG CTTTGCAGGG GAGACCTGCT TTACTAACAT CAATGAGTGC 
GAGTCTCAGC CATGTCAGAA TGGAGGTCAC TGCGTGGACC TTGTTGATGG CTTTCTTTGT 
CATTGTTTAC CAGGTTATTC AGGTGTGGAA TGTGCTGTTA ACATCAATGA GTGTGAGGAG 
GGTCCCTGCA AGAATGGAGC TGTCTGTGAG GATGGCATTG CTGACTATAC CTGCCACTGT 
GCCCCTAGCC AGGATGGCAT TGTATGGGGA GGGAAGAACT GCTCTGTCAA GCTCACTGGG 
TGCCAGACAC ATGACTGCCA AAATGAGGCC TTGTGTATCC CGACTTACCA AGCTGAGAGC 
CACGGCCACC TGTGCCAGTG CCAGCCTGGT TTCTATGATG CCACATGCTC AACACCAACA 
ACATTTTCCT TTGCTTCCAG AGGGTATCTC CTCATTGAGC TGCCCGTGAA CAATCAGAGC 
AGGAGGGAGG TAGCAGGAGA GCAGCTTGCC AGTGTGTCCC TCCGGTTCCG AACCACCTTG 
CCCAGCGCCA TCCTTTTTTA CCGAGGCCAT GAAGCTGAAT ACTTATTCCT GGAGCTCTTG 
GATGGCATTC TGCGTACAGA ACTGAAGAGG CAGGATGAGT ACCTCCTGCT GCTGGAGGAG 
CTGAGAGTTG ATGATGGCCA TTGGCATAAA GTTGAAGTTG TTCTGCAGAA CACTGTGCAG 
CTCAAACTCT GGCACGACTC CTGTGATGCA GGTGTCTGCA TGCAGAGCTC TCCTATCCCA 
CACAATGCTA CTCCGATCCC ACATGCCTTC CTGAGTGTGT ATATTGGAGG TGCTCAGGAT 
CCACTGGCCA ACAACACACA AAGCCAGCAG GGCTTTGTTG GCTGCCTGGA AGACTTGCAG 
GTGGATGCTG AAGCTGTGCT CCCGGTGGCT CTCCCTGTGA GCGAGGTTTC CTCTGTGACA 
CAGGGCTGTG ACCGCACTGA GTGGTGCCTC TCTGAGCCCT GCTTTCATGG CGGGCTGTGT 
GTGGACCTTT GGACAACGTT CAGGTGTGAC TGTTTGAGGC CGTATGGAGG CCCTGCTTGC 
TCATATGAAC ACCCAGCAGT AACATTTGGC CTGGAGAATT CTACTAGCTT TTCCTCTTTC 
ATCCTTTCTG ATAGCCTTGG TGCAGACTTC AACATCTCCT TTTTCATTCG TAGTCTGAAA 
CCTAACGGTT TACTGTTGCA AATCAGCAAT GAAACAGACC CCTGCCTCAC TATATACTTG 
AAGAATGGCA AGCTAAAGAT TGAGATGTTA TCTACAGATA CTGTGACATT TCCAGAAAAT 
TTAGTTGATG GGAGAAGACA TTTGGTAGCT CTGTCTTTCC AAGGAGGAAT TGTTGGCGTT 
CACCAACCAG ACACATATGT GGAGCTGGGA CAACTGGTGG GAAGGCCTCT TTTAGCTGGC 
TATGACGTGT ATATTGGTGG ACGTCCTGAC CCAGACAGCA CTGACCTGTG GGGAGGGTAT 
TTTAAAGGCT GCTTGCAAGA CATCCAACTG AACGGCCATC AAATAGAGTT TTTTCAGGTT 
GAGAACTACA GTCTGCCAGA TGAACTTAAT AGGACTCAAA ATATTAATTT GGTAAATGGA 
TGCATCTCTG ATAACACCTG CAAGTCTGAA CCATGTCAGA ATGGTGGAAG GTGCATTGTC 
ACGTGGAATG ATTTCCATTG CAGCTGTCCA GCAAATTTCA CTGGGAAGTT TTGTGAAGAG 
AGAGTCTGGT GTGAAAGTGA CCCATGTCCT GAAGCTACCA CCTGTATAGA TGTTCCAGCA 
GGATATGTGT GTCTGGCTAA CGCAACATTT AATAGTTCTG CTACCATTGA ATTTACCACC 
AATACATCAG TGACTAGAAC TCTGGGCAGC CTCCTCATGG ATTTCAGAAC CAGAGACGAA 
GACGCTGTTT TGCTTCGGGC TATGGAAGAA GTGGACTCCC TCCAGATAGC TATTAAGAAC 
TCCTCCTTGC TTGTTGACAT CAGGAGTGGG AATAGTATTG AAGGTGTCAG CTTCCTGAGT 
CAGAATGCTG TCACCGATGG CGCCTGGCAT ACAATCTCTC TGTCAATGGA GGAGCCTTCT 
GCACTGTCTT CCAGATGGGT GGCTCATTTG GATGGATCTA TTAATATGAC TCTGCAGGGA 
AATGCTGGGA ACCTGGACTT CTTAAAGAAC AACGCACTGA TTGTTCTAGC TGAAAACTTC 
ACAGGCTGCC TAGGACAAGT GAAGATAGGA GGGATCTACC TGCCATTCAC CTCCCATCTC 
TCATATCCTC AGCCAGAACA GTTCCAGCAG TCCAGCAGAA GAACTATCCA GTTAGGCTGC 
ACGGGGGCTG ATGTTTGTGC TTCTAGCCCT TGCAGCAATG CTGGCACCTG CAGGGACTTG 
TTCAACTCCT TCAGCTGTGC CTGCAGTGCT GGCTGGGAGG GGCTGCTGTG CGAGTCCAAT 
ACTGATGACT GCCAGTCAAG CCCATGTGTT CATGGAAACT GCACCGATAG ACTAGCAGAT 
TTTCAGTGTG AATGCTTCCG GGGATATATT GGGAAGAAGT GTGACATCAA TGTGGATGAC 
TGTGTGCGGC ACCAGTGCCA AAATGGAGCT ACCTGCATTG ATGGGGTTTA TGGCTACTCC 
TGCAAGTGCC CAGCACAGTA TTCAGGACCT CGCTGCGAAT GGCCGTTCCC ACCTGAGCAG 
TGTGGTAAGA ACTTCACCTG TCTGAATGGT GGCAAGTGTA CCACTGAGAC CTGGGGAGCC 
AACTGCACTT GCAAGCCAGG CTTCACTGGA AGGAACTTTT CCTTTCCAGT TGTCAAATTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CRB2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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