MID1 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID1
Entrez Gene ID	100468400
Full Name	midline 1
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019795057.1	XP_019650616.1	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase Midline-1

Pathways from BioSystems
Gene Ontology

The following MID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc02724	XM_019795057.1 Latest version!	Ailuropoda melanoleuca midline 1 (MID1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID1 ( XM_019795057.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650616.1 Ailuropoda melanoleuca midline 1 (MID1), mRNA.

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CloneID	OAc02724
Clone ID Related Accession (Same CDS sequence)	XM_019795057.1
Accession Version	XM_019795057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217364.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGTGCACA CAGCCTCTGC TTTAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACTGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT TACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAGGCATCC GTGAGCGGGC CCAATTCCCC TAGCGAGACC 
CGCCGGGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT ACTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTC ATCAAGAGGA ATGCGGAGCT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAGAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTCGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCG TCCCTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG GTTCCTGCAG ACGGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTCGAC 
ACCTTTGCCT TAGATTTTTC CAGAGAGAAG AAGTTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACGAT TACAGAAGAG TTCTGCACAG CTTCCTATGA TACCATCACC 
GTCCACTGGA CCTCTGATGA TGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT TGTTAGTCTG TGTCACTCTG CCGCTAGCTG GACGGTCGTG 
CCGNACATCC AGCAGAACCG TTACACGGCC CACGGTCTGC AGAGCGGCCC CAAGTACGTC 
TGCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCCGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAATG TGTTCATTGA TAGCGGTCGG 
CATTACTGGG AAGTGGTCAT AAGTGGGAGC ACATGGTATG CCATCGGCCT TGCTTACAAA 
TCGGCCCCGA AGCATGAGTG GATTGGGAAG AACTCTGCTT CTTGGGCACT GTGCCGCTGT 
CACAATAACT GGGTGGTGAG ACACAACAGT AAGGAAACCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GACAATGGTT CCATCGCCTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACCTTCT CACAGCCTGT GTGCCCCACC 
TTCACAGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TTCCAATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650616.1
CDS	169..2172
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNAELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTITEEFCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCHSAASWTVVPnullIQQNRYTAHGLQSGPKYVCIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_019795057.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca midline 1 (MID1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019795057.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGTGCACA CAGCCTCTGC TTTAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACTGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT TACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAGGCATCC GTGAGCGGGC CCAATTCCCC TAGCGAGACC 
CGCCGGGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT ACTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTC ATCAAGAGGA ATGCGGAGCT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAGAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTCGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCG TCCCTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG GTTCCTGCAG ACGGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTCGAC 
ACCTTTGCCT TAGATTTTTC CAGAGAGAAG AAGTTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACGAT TACAGAAGAG TTCTGCACAG CTTCCTATGA TACCATCACC 
GTCCACTGGA CCTCTGATGA TGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT TGTTAGTCTG TGTCACTCTG CCGCTAGCTG GACGGTCGTG 
CCGNACATCC AGCAGAACCG TTACACGGCC CACGGTCTGC AGAGCGGCCC CAAGTACGTC 
TGCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCCGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAATG TGTTCATTGA TAGCGGTCGG 
CATTACTGGG AAGTGGTCAT AAGTGGGAGC ACATGGTATG CCATCGGCCT TGCTTACAAA 
TCGGCCCCGA AGCATGAGTG GATTGGGAAG AACTCTGCTT CTTGGGCACT GTGCCGCTGT 
CACAATAACT GGGTGGTGAG ACACAACAGT AAGGAAACCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GACAATGGTT CCATCGCCTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACCTTCT CACAGCCTGT GTGCCCCACC 
TTCACAGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TTCCAATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA