Tsf1 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tsf1
Entrez Gene ID	406078
Full Name	transferrin 1
Synonyms	GB19745,Trf
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011572.1	NP_001011572.1	transferrin 1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Expression of vitellogenin and transferrin in activated ovaries of worker honey bees, Apis mellifera.
Koywiwattrakul P, Sittipraneed S
Biochemical genetics47(1-2)19-26(2009 Feb)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

Honey bee (Apis mellifera) transferrin-gene structure and the role of ecdysteroids in the developmental regulation of its expression.
do Nascimento AM, Cuvillier-Hot V, Barchuk AR, Sim?es ZL, Hartfelder K
Insect biochemistry and molecular biology34(5)415-24(2004 May)

Transcriptional profiling reveals multifunctional roles for transferrin in the honeybee, Apis mellifera.
Kucharski R, Maleszka R
Journal of insect science (Online)327(2003)

Evaluation of differential gene expression during behavioral development in the honeybee using microarrays and northern blots.
Kucharski R, Maleszka R
Genome biology3(2)RESEARCH0007(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study supports the correlation between trf and vit transcript downregulation in the activation of worker ovaries.
Title: Expression of vitellogenin and transferrin in activated ovaries of worker honey bees, Apis mellifera.

The following Tsf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tsf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00033	NM_001011572.1 Latest version!	Apis mellifera transferrin 1 (Tsf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tsf1 ( NM_001011572.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011572.1 Apis mellifera transferrin 1 (Tsf1), mRNA.

Price & Availability↑

CloneID	OHd00033
Clone ID Related Accession (Same CDS sequence)	NM_001011572.1
Accession Version	NM_001011572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Apis mellifera(honey bee)
Product	transferrin 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY336528.1. On Feb 5, 2005 this sequence version replaced XM_392054.1. ##Evidence-Data-START## Transcript exon combination :: AY336528.1, AY217097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00008880, SAMN00113341 [ECO:0000348] ##Evidence-Data-END##

ATGATGCTCC GATGCAATAT TTGGACTCTC GCGGTCAACG TGCTTTTCGT CAACTCGTTC 
CTCTTTGTCA TTGCCGCACA GGATTCCTCC GGACGAATCT TTACGATATG CGTGCCTGAA 
ATATACTCGA AGGAGTGCGA TGAGATGAAA AAAGATTCCG CTGTCAAAGG GATTCCAGTT 
TCCTGTATTT CTGGCCGCGA TCGATACGAA TGTATCGAAA AAGTTGGGAA GAAAGAGGCT 
GATGTGGTAG CCGTCGATCC GGAAGATATG TACTTGGCGG TGAAAGATAA TAAATTGGCA 
TCGAATGCTG GCTACAATGT TATCGAGCAG GTGAGAACAA AGGAGGAACC GCACGCACCG 
TATCGTTATG AAGCAGTGGC TGTGATACAC AAAGATCTGC CGATTAACAA TGTGCAAGGA 
TTGCGCGGAT TAAAGTCTTG TCACACCGGT GTGGGTCGCA ATGTTGGTTA CAAAATACCC 
ATCACCAAAC TCACCGCTAT GGGAGTGTTG AATAACCTCC ATGATCCAGA ATATTCAGCC 
CGCGAGAACG AACTGCGCGC TTTGAGTTCT CTCTTCTCCA AAGGATGCTT GGTCGGCACC 
TGGTCTCCGG ATCCAGCGAT CAATCGTAGA TTGAAGGAAA CTTATTCGAA TATGTGCGCT 
CTCTGCGAAA AACCAGAAGT TTGCGATTAT CCAGACATAT ATTCGGGATA CGAAGGTGCA 
TTGCGGTGTT TGGCGCATAA TGGCGGAGAA ATCGCTTGGA CTAAGGTGAT CTACGTGAAA 
CGCTTCTTCG GCTTACCAGT TGGTGTAACG GCGGCCATTC CGACATCGGA AAACCCAGCA 
GATTACAGAT ACTTTTGTCC AGATGGCAGC AAAGTGCCTA TAGATGCGAA CACGAAACCA 
TGCACTTGGG CTGCCAGGCC TTGGCAAGGA TACATGACTA ACAATGGTGT GAACAACGTA 
GAAGCGGTTC AAAAGGAATT GACAGATCTT GGTAAATTGG GAGAAGAAGA GAAGGCAGAT 
TGGTGGAAAG ATATTATGTT ATTGAACGAA AAAACCCTAG CCGTTCCTGC ACCGCCAGTG 
TTGCCCGAGA ATCATCTGAA AAACGCAAAA TACTTAGACG TGATTGAAAG AAACTCTGGA 
GCCACGGACA AGATCATTCG CTGGTGTACG TGGAGCGAAG GGGATCTCGA AAAGTGTAAA 
GCTCTTACCA GAGCGGCATA CTCCAGGGAC GTGAGACCCA AGTACGATTG TACCCTAGAA 
AAGAGTCAAG ACGATTGCTT GAAAGCTATT AAGGAAAATA ACGCGGATTT GACGGTTGTC 
AGCGGAGGTT CGGTTCTTCG AGCGACCAAA GAATATAACA CGGTTCCTAT AATCGCGGAA 
TCGTATGGAT CAGGCTCAAC GAATTTCAAC GAAAGGCCAG CCGTGGCAGT CGTTTCCAAG 
TCTAGCTCGA TAAACAAACT GGAGGATCTG AGGAACAAGA AATCTTGTCA CAGCGGATAC 
AAGGATAGCT TTGCTGGTTG GACAGCGCCG ATATATACAT TGAAACGCAA AGGTTTGATA 
AAATCAGAGA ACGAGGCAGC AGATTTCTTC TCAGGATCGT GTGCACCCGG TGCACCGCTC 
GATTCGAAAC TATGTCAACA ATGTGTCGGT AATCTTGCAA GCAACAACGA TCGAATCCGA 
CAGGTAACCA AATGCAAAGC GACCAACGAG GAAACGTACA GAGGTGGAAA GGGAGCTCTC 
TCTTGTCTGT TGGATGGCAA AGGAGACGTA GCGTTCGTAC CATTGACAGC TCTATCCGAG 
GAAGGCGTTC AGAGCAAAGA TCTCGCTCTG ATCTGTCCGG ATGGTGGCCG CGCTGAAATC 
AACGAGTGGG AACGATGCAA CTTGGGACTG GAACCGCCAA GAGTAATCCT CAGCTCTGGT 
GCTAAGAGTC CAACGGTCTT GGAGGAATTA ACTCACGGTA CATTAGCCGC CAGTACTCTT 
TACTCGAAGC GACCCGATCT TTTGCACTTG TTCGGTTCTT GGTCCAATCG GCCGAATCTC 
TTGTTCAAGG ACGAAGCGAA AGATCTCGTA TCGGTGAACA AATCGTGGAA CAAGTGGAAC 
GATTGGCAGG AGACACAAAA TAATTACGGT GCTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011572.1
CDS	75..2213
Translation	MMLRCNIWTLAVNVLFVNSFLFVIAAQDSSGRIFTICVPEIYSKECDEMKKDSAVKGIPVSCISGRDRYECIEKVGKKEADVVAVDPEDMYLAVKDNKLASNAGYNVIEQVRTKEEPHAPYRYEAVAVIHKDLPINNVQGLRGLKSCHTGVGRNVGYKIPITKLTAMGVLNNLHDPEYSARENELRALSSLFSKGCLVGTWSPDPAINRRLKETYSNMCALCEKPEVCDYPDIYSGYEGALRCLAHNGGEIAWTKVIYVKRFFGLPVGVTAAIPTSENPADYRYFCPDGSKVPIDANTKPCTWAARPWQGYMTNNGVNNVEAVQKELTDLGKLGEEEKADWWKDIMLLNEKTLAVPAPPVLPENHLKNAKYLDVIERNSGATDKIIRWCTWSEGDLEKCKALTRAAYSRDVRPKYDCTLEKSQDDCLKAIKENNADLTVVSGGSVLRATKEYNTVPIIAESYGSGSTNFNERPAVAVVSKSSSINKLEDLRNKKSCHSGYKDSFAGWTAPIYTLKRKGLIKSENEAADFFSGSCAPGAPLDSKLCQQCVGNLASNNDRIRQVTKCKATNEETYRGGKGALSCLLDGKGDVAFVPLTALSEEGVQSKDLALICPDGGRAEINEWERCNLGLEPPRVILSSGAKSPTVLEELTHGTLAASTLYSKRPDLLHLFGSWSNRPNLLFKDEAKDLVSVNKSWNKWNDWQETQNNYGAA

Target ORF information:

RefSeq Version	NM_001011572.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera transferrin 1 (Tsf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011572.1

ORF Insert Sequence:

ATGATGCTCC GATGCAATAT TTGGACTCTC GCGGTCAACG TGCTTTTCGT CAACTCGTTC 
CTCTTTGTCA TTGCCGCACA GGATTCCTCC GGACGAATCT TTACGATATG CGTGCCTGAA 
ATATACTCGA AGGAGTGCGA TGAGATGAAA AAAGATTCCG CTGTCAAAGG GATTCCAGTT 
TCCTGTATTT CTGGCCGCGA TCGATACGAA TGTATCGAAA AAGTTGGGAA GAAAGAGGCT 
GATGTGGTAG CCGTCGATCC GGAAGATATG TACTTGGCGG TGAAAGATAA TAAATTGGCA 
TCGAATGCTG GCTACAATGT TATCGAGCAG GTGAGAACAA AGGAGGAACC GCACGCACCG 
TATCGTTATG AAGCAGTGGC TGTGATACAC AAAGATCTGC CGATTAACAA TGTGCAAGGA 
TTGCGCGGAT TAAAGTCTTG TCACACCGGT GTGGGTCGCA ATGTTGGTTA CAAAATACCC 
ATCACCAAAC TCACCGCTAT GGGAGTGTTG AATAACCTCC ATGATCCAGA ATATTCAGCC 
CGCGAGAACG AACTGCGCGC TTTGAGTTCT CTCTTCTCCA AAGGATGCTT GGTCGGCACC 
TGGTCTCCGG ATCCAGCGAT CAATCGTAGA TTGAAGGAAA CTTATTCGAA TATGTGCGCT 
CTCTGCGAAA AACCAGAAGT TTGCGATTAT CCAGACATAT ATTCGGGATA CGAAGGTGCA 
TTGCGGTGTT TGGCGCATAA TGGCGGAGAA ATCGCTTGGA CTAAGGTGAT CTACGTGAAA 
CGCTTCTTCG GCTTACCAGT TGGTGTAACG GCGGCCATTC CGACATCGGA AAACCCAGCA 
GATTACAGAT ACTTTTGTCC AGATGGCAGC AAAGTGCCTA TAGATGCGAA CACGAAACCA 
TGCACTTGGG CTGCCAGGCC TTGGCAAGGA TACATGACTA ACAATGGTGT GAACAACGTA 
GAAGCGGTTC AAAAGGAATT GACAGATCTT GGTAAATTGG GAGAAGAAGA GAAGGCAGAT 
TGGTGGAAAG ATATTATGTT ATTGAACGAA AAAACCCTAG CCGTTCCTGC ACCGCCAGTG 
TTGCCCGAGA ATCATCTGAA AAACGCAAAA TACTTAGACG TGATTGAAAG AAACTCTGGA 
GCCACGGACA AGATCATTCG CTGGTGTACG TGGAGCGAAG GGGATCTCGA AAAGTGTAAA 
GCTCTTACCA GAGCGGCATA CTCCAGGGAC GTGAGACCCA AGTACGATTG TACCCTAGAA 
AAGAGTCAAG ACGATTGCTT GAAAGCTATT AAGGAAAATA ACGCGGATTT GACGGTTGTC 
AGCGGAGGTT CGGTTCTTCG AGCGACCAAA GAATATAACA CGGTTCCTAT AATCGCGGAA 
TCGTATGGAT CAGGCTCAAC GAATTTCAAC GAAAGGCCAG CCGTGGCAGT CGTTTCCAAG 
TCTAGCTCGA TAAACAAACT GGAGGATCTG AGGAACAAGA AATCTTGTCA CAGCGGATAC 
AAGGATAGCT TTGCTGGTTG GACAGCGCCG ATATATACAT TGAAACGCAA AGGTTTGATA 
AAATCAGAGA ACGAGGCAGC AGATTTCTTC TCAGGATCGT GTGCACCCGG TGCACCGCTC 
GATTCGAAAC TATGTCAACA ATGTGTCGGT AATCTTGCAA GCAACAACGA TCGAATCCGA 
CAGGTAACCA AATGCAAAGC GACCAACGAG GAAACGTACA GAGGTGGAAA GGGAGCTCTC 
TCTTGTCTGT TGGATGGCAA AGGAGACGTA GCGTTCGTAC CATTGACAGC TCTATCCGAG 
GAAGGCGTTC AGAGCAAAGA TCTCGCTCTG ATCTGTCCGG ATGGTGGCCG CGCTGAAATC 
AACGAGTGGG AACGATGCAA CTTGGGACTG GAACCGCCAA GAGTAATCCT CAGCTCTGGT 
GCTAAGAGTC CAACGGTCTT GGAGGAATTA ACTCACGGTA CATTAGCCGC CAGTACTCTT 
TACTCGAAGC GACCCGATCT TTTGCACTTG TTCGGTTCTT GGTCCAATCG GCCGAATCTC 
TTGTTCAAGG ACGAAGCGAA AGATCTCGTA TCGGTGAACA AATCGTGGAA CAAGTGGAAC 
GATTGGCAGG AGACACAAAA TAATTACGGT GCTGCTTAA