LOC552019 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC552019
Entrez Gene ID	552019
Full Name	rootletin
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006559389.2	XP_006559452.1	rootletin isoform X2
XM_006559391.3	XP_006559454.1	rootletin isoform X3
XM_006559391.2	XP_006559454.1	rootletin isoform X4
XM_006559389.3	XP_006559452.1	rootletin isoform X1
XM_016917087.1	XP_016772576.1	rootletin isoform X1
XM_016917088.1	XP_016772577.1	rootletin isoform X3
XM_026445731.1	XP_026301516.1	rootletin isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC552019 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC552019 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd16377	XM_026445731.1 Latest version!	Apis mellifera rootletin (LOC552019), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHd20601	XM_016917087.1 Latest version!	Apis mellifera rootletin (LOC552019), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHd20602	XM_016917088.1 Latest version!	Apis mellifera rootletin (LOC552019), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHd16376	XM_006559389.3 Latest version!	Apis mellifera rootletin (LOC552019), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OHd16378	XM_006559391.3 Latest version!	Apis mellifera rootletin (LOC552019), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OHd16378	XM_006559391.2	Apis mellifera rootletin (LOC552019), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OHd16376	XM_006559389.2	Apis mellifera rootletin (LOC552019), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC552019 ( XM_026445731.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026301516.1 Apis mellifera rootletin (LOC552019), transcript variant X2, mRNA.

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CloneID	OHd16377
Clone ID Related Accession (Same CDS sequence)	XM_026445731.1
Accession Version	XM_026445731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	rootletin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC CATTGCCTAA AAATATTTCT CAATTATCAG CAAGAGGACA GATGGAGAGG 
CGGCGACCAC CGTTTCTCAG AGGCCCTAGT AGAATGAGAG AGAAAATAGA AAGAGGCATA 
GGCAGTAAAA TGGATTCTAC AGGAAGTACA CTAAGTGGAA CTGGAGATTG TTTAAGTGAA 
GAAGATCTTT CACCAGATGC ATTGGTTAGA CAAAACTATG AATTACGTCA TCGTCTTGAA 
GAAGAAGCAG CCAATTATAA AAGACGTTTG GATACATATA GACAAGCACA ACAACATCAG 
GCTGCTCTTG TTTCACGTTT ACAAGCCAAA GTATTGCAAT ATAAACAAAA ATGTTCAGAG 
TTAGAAAATC AAATGGCAGA ATCTATAGTA TATGATTCTA ATAAAGTTGT AAGTTCTGCA 
CCTCCAACAA CTTCTGTTTT AGATGCAGCT CATCAAACAC TAAGAGAAAT ACGTGAAGAA 
CAAATATATG ATTTAGATAC TGCTCTAAAA AAACTAAGTG AAGAACGTAA AAGATGTGAA 
AAATTGCTGC AATTAAATAC AACATTGAAA GATCAGTTAG AAGAATCTCA TCAAACAAAT 
GAAGCACTTA CAAATGATCT ACAAAAATTA AGTAATGATT GGGATATTTT AAGAGAAGAA 
TTAGCTATCA AAGAAGAAGA GTGGAGAGAA GAAGAGCAAG CTTTTAATGA TTATTATACT 
TCAGAACATA ACAGATTATT AATTCTTTGG CGCGATGTTG TTTCTGTAAA ACGATTATTT 
GCAGATATAA AATCTAGTAC AGAAAGGGAT CTATTAAAAA TGAAAAATAA CATTATTTCT 
ACTTTTAATG ATGTTTCATC AGCTTGTAAT AATACAGGAT TTGCTATGAG AATGCAAGCA 
GCTATGCATC CAATGATATC TCAACAAGTT CAACAGGCAC AAGAACAGGT AACAACTGAT 
TTAAAAACAG AATTAATAAC ACTTAAACAA CAGTATGATG TAGCTCAAAA TGAAATTCGT 
ATAAAGGAAG AAAAAATCAA TCAACTTATT CGTGATGTTC ACAGTTTGGA AGAAAGATGT 
GGAGAAGCAG AAACAGAAAT TCATCGCAAT ACACGTTTGC AAGATGATAT AGAAATTTTA 
CAATCTGCAT TGAGAGATAT AGCACATGCT GTAATTCAAG ATGCAGAAAG TCGCGAAATT 
GAATCAACTC AAGCACCTCC TCATCTTCAT TTATCACCTA CTGGACCAAT TCCTCAAAAA 
TCACCAAAAA GAGGTACAAG AAGCAACACC ATTCCAGCTT TTGCTGAAAG TACTATAAGT 
GCTGTACAAG CAGCTCTCCA TAAATATCAA TTGACTATAC ATGAACTTCA GGTGAAATTG 
CAAACTAATA AAGAACAACT ACAAAATACT CGCAAACAAT GCGAAAATGC GGAAGAGAAT 
GTTAAAACTC TAGAGAAAAA AGCAGAAGAA CTGATTATTG AATTAGATGC AGTTCGATTG 
CACTGTTCAC AACTTAATCA AGAAAAAGAT ATGTTACAAA AAGGACTTGA TACTATAAGA 
ATAGAAAAAA ATACTCTTGA AAAAACCAGG ATAGAACTTA ATAATATGAT GGAGAATTTA 
AACAATGATT GCGAAAAATT GCAAAAAGCT AACAACAAAT TGCAAAAAAT TTGCGACAAT 
TTAGAAGATG AAAAATTATA TCTTCAAAGT GAACTCAATA GATTATCTAA AGATGTAGAA 
TTGAGGGAAT TAAATTTGCG TTCAGAAGAA GATAGGTGCA GTAAAATGAG AGAGGAATTA 
TTAACTCTAC GTGAAGAATT GAATAAAACA TATCTCAGTA AAGATATGTT AGAACAACAA 
AAGTTAGAAA CAGATGGATT AATTTCACAA ATTGAAAAAA GCAAAGGTGA TTTAGAATTA 
GAATTGGAAC GCGTATTATT AGAAAAATCA GACGTGCAAG AAGTTTTAAT GAAACTAGAA 
ACAGTTTGCA GCAATCATGA ACAAGAGAAA CAACGATTAC AAGAAGAATT AAAAAAGGTA 
ACAGAAGAAA AAAATAAGTT AGCAAATCAA TGTACGGATC AACAAGGTGA TCTGGGTTCC 
TTAAGAAAAG AATTACTTCA AGCAGAACAA ACTAGACTTG ATTTAGAATC AGAAAAAGTA 
ACTTTGAATG AAAAAGTGAA ATTTCTGGAG ATCGAAAAGG AGAAGATTGA AATAGAATTG 
GCACAAGTTA CGCGCGAACG TGGAGATTTC AATAATCAAT TATCAGTTTT AGCACGAAAG 
AAAGAAACAT TAAACGAAGA ATTGATGAGA CTCAAACAAA GATTAGAACA GGCAAACGAA 
ATGAATGGAA GAATAAATCG GAATTTAGAA GATCTTGTAA AAGATAATGA AGAAAAACAA 
GTTCTTTTAG AAACTAATGA AAAGGAAGTA CAACGATTAC AAGAGCAACT TGCTTCAATG 
CGTAGTGAGA AAGAGACATT AGAAGGTGTT TTATTTGATA CACAAACTAG TCTAGAAAAT 
ATGCATGTGA AAAAGACTCA ATTAGAAAAG GAACAGAAAG AACTTTTAAT AAAACAAGAA 
AGCTTAAAAG GACAAGTAGA AAGATTAATG AAAGATTTGG AGAATAGCGA AAAACGAATT 
CATGAAGTAA AGCAAACTTT AACGCAACAA AGTGGCGATC AAGAAGCCGA ATTTCAACAA 
ATTATTTCTA ACGTGAAAAA ACATAGTGAA GAAAACATAA AAAAATTAAA TGAAGAAAAA 
GAACAAATAA AGATAAATTT AGAAAAACGA CTTCAGCAAT CTCTCTTGCA AGTTACTGGA 
GAAAAAGATA ATGAAATAAA TCAATTGCAG CAAAGAATAG ATGAAATGCA ACAACATATA 
GAAAATTTGT GTCAGCAACA TGAAGAAGTT CTTCTTAGAG CAGAAAATGA TAAACAGCAA 
GCTCTTCTTA TAGCTCATCA TGATCAGCAA GCATTAATGG AAAAACTTGA AACTGTTTTG 
CATGAAATGG AAGAAGAGAA AAATAATGTA GAACGAGTCA AACGAGAAGC TGCAGTAAAA 
ACTGAACAAG AGCGTAATAA TATAAATCAA TTACGTGATG AATTAAATCG TCTTAAAACA 
AAATTAGATG AAACTAGATT AAAAACTGAT GAAGAAAAAA TAAAACTTGA TCTAAAAATA 
GAAGAACTCT GGAAAGAACG TGAATTAGCA CAAAGAGAAT CCGAAGAATT ACAAGTTCAA 
TTACATATGA CAGAAGATAA AGTTGATAGT TTACAAAATC AATTGCATGA AACTATTAGA 
AAACTTAAAG ATGCTGAAAA TCTTAACGAA ACATTACGCA AAGAATTAGT AGATATTAGA 
AGACAATTAG GTGATTCCAC ATATGAAAAA GAAAAGTATA ATAGTAGTAA TAAAGAATTA 
CGCGAGCATG TAAAACGCAT TGAAAGCGAA AAAAGAGAAC AAAATAGAAT ATTAGAAGAA 
TCATATCAAA AAATTTCAGC ACTGGAAGAT ATGAAAGTAA ATGTAGATGC AGAGAGATCT 
CGTCTTCAAG CACAAATTCG TGATATGGAG AAAGAAATGT TGCAATTACA AAAACAACTT 
CATTTTACTC AAGATGAATT ACAAAAATCA CATCAAAGTA ATGCTCAAGC ACAAAATGAT 
GAAAAAGAAT TGCAAGCTCG ACTAACTAAC GAAACAGAAG AAAGAGAACG TTTACAATTG 
CAGTTACATC AAGTAAAAAA ACAGTTAATG GATGTTGACA ATAGTCTAAA AGTAACAAGA 
CAAGAACTTG GAAGATTGCG GTCACGTGCA GATGAAGAGA ATGAACGGTG GAGAGTTAGA 
GAACAAGAAC TAGTAGTTCG TCTTGAGGAT AGTCGATGTC GTGAAAGAAA ACTCGAGGAT 
CAAAAACATA ACTTAGAAGT TTGTTTAGCT GATGCTTCTC AACAAATTCA AGAACTGAAG 
GCACGTCTTG GAGGTTCCGA AGGACGAGTT CGAGCTTTAG ATGCGCAATT ATCGCAATTA 
GAAATAACAA AAAAAGAAGT AGAACAAAAA TTAAGTAGCG TGGGATCGAC TTTACGTCGA 
ATTGCAGGTA TTCAAATGGA TGGAAGTGTT AATATACCCT TTAAATTATT GAGTCCATCA 
AGAAGGTGGA GTCCAGCAAG AGCTCAAGAT CATATTGATT CTAGTAGAGA TGTGATTCTT 
GATGTTGATC CTGAGATTAT TAGAAAAGGT GTACGATCTC TTATGCAACA AGTAGCACAA 
ATCGAACGTG AAAGAGATGA TTATAAAACT GAATTATGTA ATTTGAAAAA ACAATTGGAT 
GAAAATCAAG AGATTCAAAA TAGATCTGAT ATACAAATAA ACACTTTATT AACAAATATA 
AGAATGCTTC AGGATGAAAA GAATTCATTG GAGGTAAAAC TTTCTCAAAA ACAAAGTGGT 
TATGAAATGC AGTCAGAAGA ATGCGAGCAA TTACGCGAGA AGATTATCAA TCTTGAATTA 
ATGATTAGTA ATAATTCTGA AGAAAAAATA CAATTTGAGG AAAAACTAGA TAAATTAAAA 
CAAGTTTTAA ACAAAGTAGA AAATGAAAAA CGTAATTTAC AAGAAGAACT TAGTAAAAGC 
GAATCCCGCG CTACGAAATT AGAATTACAA AGAATGTCAT TAGAAGGTGA CCTTCAAAGA 
TTGCAAATGA TGTTCCAAGA AAAAGATGCA AATATTCATA AATTACAAGA ACGAAATGAT 
ACACAAAATC GAACAATGAC AACTTTAGAA GAACGTTGTA CTTCATTGAA ATCTATGGTG 
GAACAATTAA ATCTTGCATT GGAAAAAGCA TCTACTACAG AAAATGAATT AAAGAATGAA 
ATTAATTCAA TGCAACATAA TATTATGGAA TTAACAACTA CTTTGCAAAC TTCTAATGAA 
AAAAATAAAC AGTTACAAAA ACAAATTTCA AATGCTGAAA ATGAACGTAG AATCTTATCT 
GAACGTATAG AATCAATGCA ACAATCTTTA AATGATCTCA AACATACAAA TCAAACATTA 
ACAGATCAAA TTACACGTCT TCAAAATGAA TTGGCAAATA ATGAAGTACA ACGTTGTGCC 
TTAGAATCTC AATTAAGAAT AGTTGCCTAT CCAACACAAG AAGAAAATAT AAATAAGGAT 
GAAGAATTAT TACGACAGTT ACAAATAGCA CAAAGAGAGA GAAGTGAAAT GAGAGGAAAA 
ATGGAAGCAC TTAATGATAA AATGAAATTA TTGGAAGCTG ATAAACGTAA TTTAGAACGA 
CAACTATCGT TATTTAAATC AACTAATCGT AGCAAAAGTT ATGAACGTTA TGAGAAAGCA 
CATACAGAAT TATTAGGCAC AAGTTTCGAT ATAGATCATT ATGAACAAGA AAATAGAGAA 
TTAAGATTGA AAGTTCGTAG ATTAGAAACA CAACTTGCAG AGAAGGAAGC TGAACTTATA 
AGATTGAAAT CAAGTTACAC TCATACACAT TCCGTATTTG ATTTTAATCG AGATAGAACG 
GGCGAAATTG AAAGACTTAG AGCAGCTCAA TTACAAGCTG AAAAATTGTT AGAAGCAAGA 
GAACAAAGTC ATCGTCAACA AGTTTTGCGT TTGGAAAATC AGATACAATT GTTACGCGAG 
CAACTTAATC AAGAAATAAA ACGTCGACAA TTATATGTTT TACGAAGTTC AAGGGCAGGT 
AGAGAAATGC AACAATTAAG ACAAGCCTTA GGCGATTCTT TAAGAACTGT AGCTCAAGAT 
CCATCATTAG ACGCAGTATT ATTAGAACAT GAAGCTCGAA AATTGGATTC TACTTTGACA 
AGTACTACCA GTTTACCACC ATCATTAGCT TTACCTCCGC CACCGTCTTA TGATAGATCT 
TCCACACCAG CACAACTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026301516.1
CDS	86..5989
Translation	MSRPLPKNISQLSARGQMERRRPPFLRGPSRMREKIERGIGSKMDSTGSTLSGTGDCLSEEDLSPDALVRQNYELRHRLEEEAANYKRRLDTYRQAQQHQAALVSRLQAKVLQYKQKCSELENQMAESIVYDSNKVVSSAPPTTSVLDAAHQTLREIREEQIYDLDTALKKLSEERKRCEKLLQLNTTLKDQLEESHQTNEALTNDLQKLSNDWDILREELAIKEEEWREEEQAFNDYYTSEHNRLLILWRDVVSVKRLFADIKSSTERDLLKMKNNIISTFNDVSSACNNTGFAMRMQAAMHPMISQQVQQAQEQVTTDLKTELITLKQQYDVAQNEIRIKEEKINQLIRDVHSLEERCGEAETEIHRNTRLQDDIEILQSALRDIAHAVIQDAESREIESTQAPPHLHLSPTGPIPQKSPKRGTRSNTIPAFAESTISAVQAALHKYQLTIHELQVKLQTNKEQLQNTRKQCENAEENVKTLEKKAEELIIELDAVRLHCSQLNQEKDMLQKGLDTIRIEKNTLEKTRIELNNMMENLNNDCEKLQKANNKLQKICDNLEDEKLYLQSELNRLSKDVELRELNLRSEEDRCSKMREELLTLREELNKTYLSKDMLEQQKLETDGLISQIEKSKGDLELELERVLLEKSDVQEVLMKLETVCSNHEQEKQRLQEELKKVTEEKNKLANQCTDQQGDLGSLRKELLQAEQTRLDLESEKVTLNEKVKFLEIEKEKIEIELAQVTRERGDFNNQLSVLARKKETLNEELMRLKQRLEQANEMNGRINRNLEDLVKDNEEKQVLLETNEKEVQRLQEQLASMRSEKETLEGVLFDTQTSLENMHVKKTQLEKEQKELLIKQESLKGQVERLMKDLENSEKRIHEVKQTLTQQSGDQEAEFQQIISNVKKHSEENIKKLNEEKEQIKINLEKRLQQSLLQVTGEKDNEINQLQQRIDEMQQHIENLCQQHEEVLLRAENDKQQALLIAHHDQQALMEKLETVLHEMEEEKNNVERVKREAAVKTEQERNNINQLRDELNRLKTKLDETRLKTDEEKIKLDLKIEELWKERELAQRESEELQVQLHMTEDKVDSLQNQLHETIRKLKDAENLNETLRKELVDIRRQLGDSTYEKEKYNSSNKELREHVKRIESEKREQNRILEESYQKISALEDMKVNVDAERSRLQAQIRDMEKEMLQLQKQLHFTQDELQKSHQSNAQAQNDEKELQARLTNETEERERLQLQLHQVKKQLMDVDNSLKVTRQELGRLRSRADEENERWRVREQELVVRLEDSRCRERKLEDQKHNLEVCLADASQQIQELKARLGGSEGRVRALDAQLSQLEITKKEVEQKLSSVGSTLRRIAGIQMDGSVNIPFKLLSPSRRWSPARAQDHIDSSRDVILDVDPEIIRKGVRSLMQQVAQIERERDDYKTELCNLKKQLDENQEIQNRSDIQINTLLTNIRMLQDEKNSLEVKLSQKQSGYEMQSEECEQLREKIINLELMISNNSEEKIQFEEKLDKLKQVLNKVENEKRNLQEELSKSESRATKLELQRMSLEGDLQRLQMMFQEKDANIHKLQERNDTQNRTMTTLEERCTSLKSMVEQLNLALEKASTTENELKNEINSMQHNIMELTTTLQTSNEKNKQLQKQISNAENERRILSERIESMQQSLNDLKHTNQTLTDQITRLQNELANNEVQRCALESQLRIVAYPTQEENINKDEELLRQLQIAQRERSEMRGKMEALNDKMKLLEADKRNLERQLSLFKSTNRSKSYERYEKAHTELLGTSFDIDHYEQENRELRLKVRRLETQLAEKEAELIRLKSSYTHTHSVFDFNRDRTGEIERLRAAQLQAEKLLEAREQSHRQQVLRLENQIQLLREQLNQEIKRRQLYVLRSSRAGREMQQLRQALGDSLRTVAQDPSLDAVLLEHEARKLDSTLTSTTSLPPSLALPPPPSYDRSSTPAQLK

Target ORF information:

RefSeq Version	XM_026445731.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera rootletin (LOC552019), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026445731.1

ORF Insert Sequence:

ATGTCACGGC CATTGCCTAA AAATATTTCT CAATTATCAG CAAGAGGACA GATGGAGAGG 
CGGCGACCAC CGTTTCTCAG AGGCCCTAGT AGAATGAGAG AGAAAATAGA AAGAGGCATA 
GGCAGTAAAA TGGATTCTAC AGGAAGTACA CTAAGTGGAA CTGGAGATTG TTTAAGTGAA 
GAAGATCTTT CACCAGATGC ATTGGTTAGA CAAAACTATG AATTACGTCA TCGTCTTGAA 
GAAGAAGCAG CCAATTATAA AAGACGTTTG GATACATATA GACAAGCACA ACAACATCAG 
GCTGCTCTTG TTTCACGTTT ACAAGCCAAA GTATTGCAAT ATAAACAAAA ATGTTCAGAG 
TTAGAAAATC AAATGGCAGA ATCTATAGTA TATGATTCTA ATAAAGTTGT AAGTTCTGCA 
CCTCCAACAA CTTCTGTTTT AGATGCAGCT CATCAAACAC TAAGAGAAAT ACGTGAAGAA 
CAAATATATG ATTTAGATAC TGCTCTAAAA AAACTAAGTG AAGAACGTAA AAGATGTGAA 
AAATTGCTGC AATTAAATAC AACATTGAAA GATCAGTTAG AAGAATCTCA TCAAACAAAT 
GAAGCACTTA CAAATGATCT ACAAAAATTA AGTAATGATT GGGATATTTT AAGAGAAGAA 
TTAGCTATCA AAGAAGAAGA GTGGAGAGAA GAAGAGCAAG CTTTTAATGA TTATTATACT 
TCAGAACATA ACAGATTATT AATTCTTTGG CGCGATGTTG TTTCTGTAAA ACGATTATTT 
GCAGATATAA AATCTAGTAC AGAAAGGGAT CTATTAAAAA TGAAAAATAA CATTATTTCT 
ACTTTTAATG ATGTTTCATC AGCTTGTAAT AATACAGGAT TTGCTATGAG AATGCAAGCA 
GCTATGCATC CAATGATATC TCAACAAGTT CAACAGGCAC AAGAACAGGT AACAACTGAT 
TTAAAAACAG AATTAATAAC ACTTAAACAA CAGTATGATG TAGCTCAAAA TGAAATTCGT 
ATAAAGGAAG AAAAAATCAA TCAACTTATT CGTGATGTTC ACAGTTTGGA AGAAAGATGT 
GGAGAAGCAG AAACAGAAAT TCATCGCAAT ACACGTTTGC AAGATGATAT AGAAATTTTA 
CAATCTGCAT TGAGAGATAT AGCACATGCT GTAATTCAAG ATGCAGAAAG TCGCGAAATT 
GAATCAACTC AAGCACCTCC TCATCTTCAT TTATCACCTA CTGGACCAAT TCCTCAAAAA 
TCACCAAAAA GAGGTACAAG AAGCAACACC ATTCCAGCTT TTGCTGAAAG TACTATAAGT 
GCTGTACAAG CAGCTCTCCA TAAATATCAA TTGACTATAC ATGAACTTCA GGTGAAATTG 
CAAACTAATA AAGAACAACT ACAAAATACT CGCAAACAAT GCGAAAATGC GGAAGAGAAT 
GTTAAAACTC TAGAGAAAAA AGCAGAAGAA CTGATTATTG AATTAGATGC AGTTCGATTG 
CACTGTTCAC AACTTAATCA AGAAAAAGAT ATGTTACAAA AAGGACTTGA TACTATAAGA 
ATAGAAAAAA ATACTCTTGA AAAAACCAGG ATAGAACTTA ATAATATGAT GGAGAATTTA 
AACAATGATT GCGAAAAATT GCAAAAAGCT AACAACAAAT TGCAAAAAAT TTGCGACAAT 
TTAGAAGATG AAAAATTATA TCTTCAAAGT GAACTCAATA GATTATCTAA AGATGTAGAA 
TTGAGGGAAT TAAATTTGCG TTCAGAAGAA GATAGGTGCA GTAAAATGAG AGAGGAATTA 
TTAACTCTAC GTGAAGAATT GAATAAAACA TATCTCAGTA AAGATATGTT AGAACAACAA 
AAGTTAGAAA CAGATGGATT AATTTCACAA ATTGAAAAAA GCAAAGGTGA TTTAGAATTA 
GAATTGGAAC GCGTATTATT AGAAAAATCA GACGTGCAAG AAGTTTTAAT GAAACTAGAA 
ACAGTTTGCA GCAATCATGA ACAAGAGAAA CAACGATTAC AAGAAGAATT AAAAAAGGTA 
ACAGAAGAAA AAAATAAGTT AGCAAATCAA TGTACGGATC AACAAGGTGA TCTGGGTTCC 
TTAAGAAAAG AATTACTTCA AGCAGAACAA ACTAGACTTG ATTTAGAATC AGAAAAAGTA 
ACTTTGAATG AAAAAGTGAA ATTTCTGGAG ATCGAAAAGG AGAAGATTGA AATAGAATTG 
GCACAAGTTA CGCGCGAACG TGGAGATTTC AATAATCAAT TATCAGTTTT AGCACGAAAG 
AAAGAAACAT TAAACGAAGA ATTGATGAGA CTCAAACAAA GATTAGAACA GGCAAACGAA 
ATGAATGGAA GAATAAATCG GAATTTAGAA GATCTTGTAA AAGATAATGA AGAAAAACAA 
GTTCTTTTAG AAACTAATGA AAAGGAAGTA CAACGATTAC AAGAGCAACT TGCTTCAATG 
CGTAGTGAGA AAGAGACATT AGAAGGTGTT TTATTTGATA CACAAACTAG TCTAGAAAAT 
ATGCATGTGA AAAAGACTCA ATTAGAAAAG GAACAGAAAG AACTTTTAAT AAAACAAGAA 
AGCTTAAAAG GACAAGTAGA AAGATTAATG AAAGATTTGG AGAATAGCGA AAAACGAATT 
CATGAAGTAA AGCAAACTTT AACGCAACAA AGTGGCGATC AAGAAGCCGA ATTTCAACAA 
ATTATTTCTA ACGTGAAAAA ACATAGTGAA GAAAACATAA AAAAATTAAA TGAAGAAAAA 
GAACAAATAA AGATAAATTT AGAAAAACGA CTTCAGCAAT CTCTCTTGCA AGTTACTGGA 
GAAAAAGATA ATGAAATAAA TCAATTGCAG CAAAGAATAG ATGAAATGCA ACAACATATA 
GAAAATTTGT GTCAGCAACA TGAAGAAGTT CTTCTTAGAG CAGAAAATGA TAAACAGCAA 
GCTCTTCTTA TAGCTCATCA TGATCAGCAA GCATTAATGG AAAAACTTGA AACTGTTTTG 
CATGAAATGG AAGAAGAGAA AAATAATGTA GAACGAGTCA AACGAGAAGC TGCAGTAAAA 
ACTGAACAAG AGCGTAATAA TATAAATCAA TTACGTGATG AATTAAATCG TCTTAAAACA 
AAATTAGATG AAACTAGATT AAAAACTGAT GAAGAAAAAA TAAAACTTGA TCTAAAAATA 
GAAGAACTCT GGAAAGAACG TGAATTAGCA CAAAGAGAAT CCGAAGAATT ACAAGTTCAA 
TTACATATGA CAGAAGATAA AGTTGATAGT TTACAAAATC AATTGCATGA AACTATTAGA 
AAACTTAAAG ATGCTGAAAA TCTTAACGAA ACATTACGCA AAGAATTAGT AGATATTAGA 
AGACAATTAG GTGATTCCAC ATATGAAAAA GAAAAGTATA ATAGTAGTAA TAAAGAATTA 
CGCGAGCATG TAAAACGCAT TGAAAGCGAA AAAAGAGAAC AAAATAGAAT ATTAGAAGAA 
TCATATCAAA AAATTTCAGC ACTGGAAGAT ATGAAAGTAA ATGTAGATGC AGAGAGATCT 
CGTCTTCAAG CACAAATTCG TGATATGGAG AAAGAAATGT TGCAATTACA AAAACAACTT 
CATTTTACTC AAGATGAATT ACAAAAATCA CATCAAAGTA ATGCTCAAGC ACAAAATGAT 
GAAAAAGAAT TGCAAGCTCG ACTAACTAAC GAAACAGAAG AAAGAGAACG TTTACAATTG 
CAGTTACATC AAGTAAAAAA ACAGTTAATG GATGTTGACA ATAGTCTAAA AGTAACAAGA 
CAAGAACTTG GAAGATTGCG GTCACGTGCA GATGAAGAGA ATGAACGGTG GAGAGTTAGA 
GAACAAGAAC TAGTAGTTCG TCTTGAGGAT AGTCGATGTC GTGAAAGAAA ACTCGAGGAT 
CAAAAACATA ACTTAGAAGT TTGTTTAGCT GATGCTTCTC AACAAATTCA AGAACTGAAG 
GCACGTCTTG GAGGTTCCGA AGGACGAGTT CGAGCTTTAG ATGCGCAATT ATCGCAATTA 
GAAATAACAA AAAAAGAAGT AGAACAAAAA TTAAGTAGCG TGGGATCGAC TTTACGTCGA 
ATTGCAGGTA TTCAAATGGA TGGAAGTGTT AATATACCCT TTAAATTATT GAGTCCATCA 
AGAAGGTGGA GTCCAGCAAG AGCTCAAGAT CATATTGATT CTAGTAGAGA TGTGATTCTT 
GATGTTGATC CTGAGATTAT TAGAAAAGGT GTACGATCTC TTATGCAACA AGTAGCACAA 
ATCGAACGTG AAAGAGATGA TTATAAAACT GAATTATGTA ATTTGAAAAA ACAATTGGAT 
GAAAATCAAG AGATTCAAAA TAGATCTGAT ATACAAATAA ACACTTTATT AACAAATATA 
AGAATGCTTC AGGATGAAAA GAATTCATTG GAGGTAAAAC TTTCTCAAAA ACAAAGTGGT 
TATGAAATGC AGTCAGAAGA ATGCGAGCAA TTACGCGAGA AGATTATCAA TCTTGAATTA 
ATGATTAGTA ATAATTCTGA AGAAAAAATA CAATTTGAGG AAAAACTAGA TAAATTAAAA 
CAAGTTTTAA ACAAAGTAGA AAATGAAAAA CGTAATTTAC AAGAAGAACT TAGTAAAAGC 
GAATCCCGCG CTACGAAATT AGAATTACAA AGAATGTCAT TAGAAGGTGA CCTTCAAAGA 
TTGCAAATGA TGTTCCAAGA AAAAGATGCA AATATTCATA AATTACAAGA ACGAAATGAT 
ACACAAAATC GAACAATGAC AACTTTAGAA GAACGTTGTA CTTCATTGAA ATCTATGGTG 
GAACAATTAA ATCTTGCATT GGAAAAAGCA TCTACTACAG AAAATGAATT AAAGAATGAA 
ATTAATTCAA TGCAACATAA TATTATGGAA TTAACAACTA CTTTGCAAAC TTCTAATGAA 
AAAAATAAAC AGTTACAAAA ACAAATTTCA AATGCTGAAA ATGAACGTAG AATCTTATCT 
GAACGTATAG AATCAATGCA ACAATCTTTA AATGATCTCA AACATACAAA TCAAACATTA 
ACAGATCAAA TTACACGTCT TCAAAATGAA TTGGCAAATA ATGAAGTACA ACGTTGTGCC 
TTAGAATCTC AATTAAGAAT AGTTGCCTAT CCAACACAAG AAGAAAATAT AAATAAGGAT 
GAAGAATTAT TACGACAGTT ACAAATAGCA CAAAGAGAGA GAAGTGAAAT GAGAGGAAAA 
ATGGAAGCAC TTAATGATAA AATGAAATTA TTGGAAGCTG ATAAACGTAA TTTAGAACGA 
CAACTATCGT TATTTAAATC AACTAATCGT AGCAAAAGTT ATGAACGTTA TGAGAAAGCA 
CATACAGAAT TATTAGGCAC AAGTTTCGAT ATAGATCATT ATGAACAAGA AAATAGAGAA 
TTAAGATTGA AAGTTCGTAG ATTAGAAACA CAACTTGCAG AGAAGGAAGC TGAACTTATA 
AGATTGAAAT CAAGTTACAC TCATACACAT TCCGTATTTG ATTTTAATCG AGATAGAACG 
GGCGAAATTG AAAGACTTAG AGCAGCTCAA TTACAAGCTG AAAAATTGTT AGAAGCAAGA 
GAACAAAGTC ATCGTCAACA AGTTTTGCGT TTGGAAAATC AGATACAATT GTTACGCGAG 
CAACTTAATC AAGAAATAAA ACGTCGACAA TTATATGTTT TACGAAGTTC AAGGGCAGGT 
AGAGAAATGC AACAATTAAG ACAAGCCTTA GGCGATTCTT TAAGAACTGT AGCTCAAGAT 
CCATCATTAG ACGCAGTATT ATTAGAACAT GAAGCTCGAA AATTGGATTC TACTTTGACA 
AGTACTACCA GTTTACCACC ATCATTAGCT TTACCTCCGC CACCGTCTTA TGATAGATCT 
TCCACACCAG CACAACTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552019 ( XM_016917087.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016772576.1 Apis mellifera rootletin (LOC552019), transcript variant X1, mRNA.

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CloneID	OHd20601
Clone ID Related Accession (Same CDS sequence)	XM_016917087.1
Accession Version	XM_016917087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	rootletin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007085.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGAA TGCCATTGCC TAAAAATATT TCTCAATTAT CAGCAAGAGG ACAGATGGAG 
AGGCGGCGAC CACCGTTTCT CAGAGGCCCT AGTAGAATGA GAGAGAAAAT AGAAAGAGGC 
ATAGGCAGTA AAATGGATTC TACAGGAAGT ACACTAAGTG GAACTGGAGA TTGTTTAAGT 
GAAGAAGATC TTTCACCAGA TGCATTGGTT AGACAAAACT ATGAATTACG TCATCGTCTT 
GAAGAAGAAG CAGCCAATTA TAAAAGACGT TTGGATACAT ATAGACAAGC ACAACAACAT 
CAGGCTGCTC TTGTTTCACG TTTACAAGCC AAAGTATTGC AATATAAACA AAAATGTTCA 
GAGTTAGAAA ATCAAATGGC AGAATCTATA GTATATGATT CTAATAAAGT TGTAAGTTCT 
GCACCTCCAA CAACTTCTGT TTTAGATGCA GCTCATCAAA CACTAAGAGA AATACGTGAA 
GAACAAATAT ATGATTTAGA TACTGCTCTA AAAAAACTAA GTGAAGAACG TAAAAGATGT 
GAAAAATTGC TGCAATTAAA TACAACATTG AAAGATCAGT TAGAAGAATC TCATCAAACA 
AATGAAGCAC TTACAAATGA TCTACAAAAA TTAAGTAATG ATTGGGATAT TTTAAGAGAA 
GAATTAGCTA TCAAAGAAGA AGAGTGGAGA GAAGAAGAGC AAGCTTTTAA TGATTATTAT 
ACTTCAGAAC ATAACAGATT ATTAATTCTT TGGCGCGATG TTGTTTCTGT AAAACGATTA 
TTTGCAGATA TAAAATCTAG TACAGAAAGG GATCTATTAA AAATGAAAAA TAACATTATT 
TCTACTTTTA ATGATGTTTC ATCAGCTTGT AATAATACAG GATTTGCTAT GAGAATGCAA 
GCAGCTATGC ATCCAATGAT ATCTCAACAA GTTCAACAGG CACAAGAACA GGTAACAACT 
GATTTAAAAA CAGAATTAAT AACACTTAAA CAACAGTATG ATGTAGCTCA AAATGAAATT 
CGTATAAAGG AAGAAAAAAT CAATCAACTT ATTCGTGATG TTCACAGTTT GGAAGAAAGA 
TGTGGAGAAG CAGAAACAGA AATTCATCGC AATACACGTT TGCAAGATGA TATAGAAATT 
TTACAATCTG CATTGAGAGA TATAGCACAT GCTGTAATTC AAGATGCAGA AAGTCGCGAA 
ATTGAATCAA CTCAAGCACC TCCTCATCTT CATTTATCAC CTACTGGACC AATTCCTCAA 
AAATCACCAA AAAGAGGTAC AAGAAGCAAC ACCATTCCAG CTTTTGCTGA AAGTACTATA 
AGTGCTGTAC AAGCAGCTCT CCATAAATAT CAATTGACTA TACATGAACT TCAGGTGAAA 
TTGCAAACTA ATAAAGAACA ACTACAAAAT ACTCGCAAAC AATGCGAAAA TGCGGAAGAG 
AATGTTAAAA CTCTAGAGAA AAAAGCAGAA GAACTGATTA TTGAATTAGA TGCAGTTCGA 
TTGCACTGTT CACAACTTAA TCAAGAAAAA GATATGTTAC AAAAAGGACT TGATACTATA 
AGAATAGAAA AAAATACTCT TGAAAAAACC AGGATAGAAC TTAATAATAT GATGGAGAAT 
TTAAACAATG ATTGCGAAAA ATTGCAAAAA GCTAACAACA AATTGCAAAA AATTTGCGAC 
AATTTAGAAG ATGAAAAATT ATATCTTCAA AGTGAACTCA ATAGATTATC TAAAGATGTA 
GAATTGAGGG AATTAAATTT GCGTTCAGAA GAAGATAGGT GCAGTAAAAT GAGAGAGGAA 
TTATTAACTC TACGTGAAGA ATTGAATAAA ACATATCTCA GTAAAGATAT GTTAGAACAA 
CAAAAGTTAG AAACAGATGG ATTAATTTCA CAAATTGAAA AAAGCAAAGG TGATTTAGAA 
TTAGAATTGG AACGCGTATT ATTAGAAAAA TCAGACGTGC AAGAAGTTTT AATGAAACTA 
GAAACAGTTT GCAGCAATCA TGAACAAGAG AAACAACGAT TACAAGAAGA ATTAAAAAAG 
GTAACAGAAG AAAAAAATAA GTTAGCAAAT CAATGTACGG ATCAACAAGG TGATCTGGGT 
TCCTTAAGAA AAGAATTACT TCAAGCAGAA CAAACTAGAC TTGATTTAGA ATCAGAAAAA 
GTAACTTTGA ATGAAAAAGT GAAATTTCTG GAGATCGAAA AGGAGAAGAT TGAAATAGAA 
TTGGCACAAG TTACGCGCGA ACGTGGAGAT TTCAATAATC AATTATCAGT TTTAGCACGA 
AAGAAAGAAA CATTAAACGA AGAATTGATG AGACTCAAAC AAAGATTAGA ACAGGCAAAC 
GAAATGAATG GAAGAATAAA TCGGAATTTA GAAGATCTTG TAAAAGATAA TGAAGAAAAA 
CAAGTTCTTT TAGAAACTAA TGAAAAGGAA GTACAACGAT TACAAGAGCA ACTTGCTTCA 
ATGCGTAGTG AGAAAGAGAC ATTAGAAGGT GTTTTATTTG ATACACAAAC TAGTCTAGAA 
AATATGCATG TGAAAAAGAC TCAATTAGAA AAGGAACAGA AAGAACTTTT AATAAAACAA 
GAAAGCTTAA AAGGACAAGT AGAAAGATTA ATGAAAGATT TGGAGAATAG CGAAAAACGA 
ATTCATGAAG TAAAGCAAAC TTTAACGCAA CAAAGTGGCG ATCAAGAAGC CGAATTTCAA 
CAAATTATTT CTAACGTGAA AAAACATAGT GAAGAAAACA TAAAAAAATT AAATGAAGAA 
AAAGAACAAA TAAAGATAAA TTTAGAAAAA CGACTTCAGC AATCTCTCTT GCAAGTTACT 
GGAGAAAAAG ATAATGAAAT AAATCAATTG CAGCAAAGAA TAGATGAAAT GCAACAACAT 
ATAGAAAATT TGTGTCAGCA ACATGAAGAA GTTCTTCTTA GAGCAGAAAA TGATAAACAG 
CAAGCTCTTC TTATAGCTCA TCATGATCAG CAAGCATTAA TGGAAAAACT TGAAACTGTT 
TTGCATGAAA TGGAAGAAGA GAAAAATAAT GTAGAACGAG TCAAACGAGA AGCTGCAGTA 
AAAACTGAAC AAGAGCGTAA TAATATAAAT CAATTACGTG ATGAATTAAA TCGTCTTAAA 
ACAAAATTAG ATGAAACTAG ATTAAAAACT GATGAAGAAA AAATAAAACT TGATCTAAAA 
ATAGAAGAAC TCTGGAAAGA ACGTGAATTA GCACAAAGAG AATCCGAAGA ATTACAAGTT 
CAATTACATA TGACAGAAGA TAAAGTTGAT AGTTTACAAA ATCAATTGCA TGAAACTATT 
AGAAAACTTA AAGATGCTGA AAATCTTAAC GAAACATTAC GCAAAGAATT AGTAGATATT 
AGAAGACAAT TAGGTGATTC CACATATGAA AAAGAAAAGT ATAATAGTAG TAATAAAGAA 
TTACGCGAGC ATGTAAAACG CATTGAAAGC GAAAAAAGAG AACAAAATAG AATATTAGAA 
GAATCATATC AAAAAATTTC AGCACTGGAA GATATGAAAG TAAATGTAGA TGCAGAGAGA 
TCTCGTCTTC AAGCACAAAT TCGTGATATG GAGAAAGAAA TGTTGCAATT ACAAAAACAA 
CTTCATTTTA CTCAAGATGA ATTACAAAAA TCACATCAAA GTAATGCTCA AGCACAAAAT 
GATGAAAAAG AATTGCAAGC TCGACTAACT AACGAAACAG AAGAAAGAGA ACGTTTACAA 
TTGCAGTTAC ATCAAGTAAA AAAACAGTTA ATGGATGTTG ACAATAGTCT AAAAGTAACA 
AGACAAGAAC TTGGAAGATT GCGGTCACGT GCAGATGAAG AGAATGAACG GTGGAGAGTT 
AGAGAACAAG AACTAGTAGT TCGTCTTGAG GATAGTCGAT GTCGTGAAAG AAAACTCGAG 
GATCAAAAAC ATAACTTAGA AGTTTGTTTA GCTGATGCTT CTCAACAAAT TCAAGAACTG 
AAGGCACGTC TTGGAGGTTC CGAAGGACGA GTTCGAGCTT TAGATGCGCA ATTATCGCAA 
TTAGAAATAA CAAAAAAAGA AGTAGAACAA AAATTAAGTA GCGTGGGATC GACTTTACGT 
CGAATTGCAG GTATTCAAAT GGATGGAAGT GTTAATATAC CCTTTAAATT ATTGAGTCCA 
TCAAGAAGGT GGAGTCCAGC AAGAGCTCAA GATCATATTG ATTCTAGTAG AGATGTGATT 
CTTGATGTTG ATCCTGAGAT TATTAGAAAA GGTGTACGAT CTCTTATGCA ACAAGTAGCA 
CAAATCGAAC GTGAAAGAGA TGATTATAAA ACTGAATTAT GTAATTTGAA AAAACAATTG 
GATGAAAATC AAGAGATTCA AAATAGATCT GATATACAAA TAAACACTTT ATTAACAAAT 
ATAAGAATGC TTCAGGATGA AAAGAATTCA TTGGAGGTAA AACTTTCTCA AAAACAAAGT 
GGTTATGAAA TGCAGTTAAA TGCTTTACAA CTAAAGTCAG AAGAATGCGA GCAATTACGC 
GAGAAGATTA TCAATCTTGA ATTAATGATT AGTAATAATT CTGAAGAAAA AATACAATTT 
GAGGAAAAAC TAGATAAATT AAAACAAGTT TTAAACAAAG TAGAAAATGA AAAACGTAAT 
TTACAAGAAG AACTTAGTAA AAGCGAATCC CGCGCTACGA AATTAGAATT ACAAAGAATG 
TCATTAGAAG GTGACCTTCA AAGATTGCAA ATGATGTTCC AAGAAAAAGA TGCAAATATT 
CATAAATTAC AAGAACGAAA TGATACACAA AATCGAACAA TGACAACTTT AGAAGAACGT 
TGTACTTCAT TGAAATCTAT GGTGGAACAA TTAAATCTTG CATTGGAAAA AGCATCTACT 
ACAGAAAATG AATTAAAGAA TGAAATTAAT TCAATGCAAC ATAATATTAT GGAATTAACA 
ACTACTTTGC AAACTTCTAA TGAAAAAAAT AAACAGTTAC AAAAACAAAT TTCAAATGCT 
GAAAATGAAC GTAGAATCTT ATCTGAACGT ATAGAATCAA TGCAACAATC TTTAAATGAT 
CTCAAACATA CAAATCAAAC ATTAACAGAT CAAATTACAC GTCTTCAAAA TGAATTGGCA 
AATAATGAAG TACAACGTTG TGCCTTAGAA TCTCAATTAA GAATAGTTGC CTATCCAACA 
CAAGAAGAAA ATATAAATAA GGATGAAGAA TTATTACGAC AGTTACAAAT AGCACAAAGA 
GAGAGAAGTG AAATGAGAGG AAAAATGGAA GCACTTAATG ATAAAATGAA ATTATTGGAA 
GCTGATAAAC GTAATTTAGA ACGACAACTA TCGTTATTTA AATCAACTAA TCGTAGCAAA 
AGTTATGAAC GTTATGAGAA AGCACATACA GAATTATTAG GCACAAGTTT CGATATAGAT 
CATTATGAAC AAGAAAATAG AGAATTAAGA TTGAAAGTTC GTAGATTAGA AACACAACTT 
GCAGAGAAGG AAGCTGAACT TATAAGATTG AAATCAAGTT ACACTCATAC ACATTCCGTA 
TTTGATTTTA ATCGAGATAG AACGGGCGAA ATTGAAAGAC TTAGAGCAGC TCAATTACAA 
GCTGAAAAAT TGTTAGAAGC AAGAGAACAA AGTCATCGTC AACAAGTTTT GCGTTTGGAA 
AATCAGATAC AATTGTTACG CGAGCAACTT AATCAAGAAA TAAAACGTCG ACAATTATAT 
GTTTTACGAA GTTCAAGGGC AGGTAGAGAA ATGCAACAAT TAAGACAAGC CTTAGGCGAT 
TCTTTAAGAA CTGTAGCTCA AGATCCATCA TTAGACGCAG TATTATTAGA ACATGAAGCT 
CGAAAATTGG ATTCTACTTT GACAAGTACT ACCAGTTTAC CACCATCATT AGCTTTACCT 
CCGCCACCGT CTTATGATAG ATCTTCCACA CCAGCACAAC TCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016772576.1
CDS	175..6102
Translation	MRRMPLPKNISQLSARGQMERRRPPFLRGPSRMREKIERGIGSKMDSTGSTLSGTGDCLSEEDLSPDALVRQNYELRHRLEEEAANYKRRLDTYRQAQQHQAALVSRLQAKVLQYKQKCSELENQMAESIVYDSNKVVSSAPPTTSVLDAAHQTLREIREEQIYDLDTALKKLSEERKRCEKLLQLNTTLKDQLEESHQTNEALTNDLQKLSNDWDILREELAIKEEEWREEEQAFNDYYTSEHNRLLILWRDVVSVKRLFADIKSSTERDLLKMKNNIISTFNDVSSACNNTGFAMRMQAAMHPMISQQVQQAQEQVTTDLKTELITLKQQYDVAQNEIRIKEEKINQLIRDVHSLEERCGEAETEIHRNTRLQDDIEILQSALRDIAHAVIQDAESREIESTQAPPHLHLSPTGPIPQKSPKRGTRSNTIPAFAESTISAVQAALHKYQLTIHELQVKLQTNKEQLQNTRKQCENAEENVKTLEKKAEELIIELDAVRLHCSQLNQEKDMLQKGLDTIRIEKNTLEKTRIELNNMMENLNNDCEKLQKANNKLQKICDNLEDEKLYLQSELNRLSKDVELRELNLRSEEDRCSKMREELLTLREELNKTYLSKDMLEQQKLETDGLISQIEKSKGDLELELERVLLEKSDVQEVLMKLETVCSNHEQEKQRLQEELKKVTEEKNKLANQCTDQQGDLGSLRKELLQAEQTRLDLESEKVTLNEKVKFLEIEKEKIEIELAQVTRERGDFNNQLSVLARKKETLNEELMRLKQRLEQANEMNGRINRNLEDLVKDNEEKQVLLETNEKEVQRLQEQLASMRSEKETLEGVLFDTQTSLENMHVKKTQLEKEQKELLIKQESLKGQVERLMKDLENSEKRIHEVKQTLTQQSGDQEAEFQQIISNVKKHSEENIKKLNEEKEQIKINLEKRLQQSLLQVTGEKDNEINQLQQRIDEMQQHIENLCQQHEEVLLRAENDKQQALLIAHHDQQALMEKLETVLHEMEEEKNNVERVKREAAVKTEQERNNINQLRDELNRLKTKLDETRLKTDEEKIKLDLKIEELWKERELAQRESEELQVQLHMTEDKVDSLQNQLHETIRKLKDAENLNETLRKELVDIRRQLGDSTYEKEKYNSSNKELREHVKRIESEKREQNRILEESYQKISALEDMKVNVDAERSRLQAQIRDMEKEMLQLQKQLHFTQDELQKSHQSNAQAQNDEKELQARLTNETEERERLQLQLHQVKKQLMDVDNSLKVTRQELGRLRSRADEENERWRVREQELVVRLEDSRCRERKLEDQKHNLEVCLADASQQIQELKARLGGSEGRVRALDAQLSQLEITKKEVEQKLSSVGSTLRRIAGIQMDGSVNIPFKLLSPSRRWSPARAQDHIDSSRDVILDVDPEIIRKGVRSLMQQVAQIERERDDYKTELCNLKKQLDENQEIQNRSDIQINTLLTNIRMLQDEKNSLEVKLSQKQSGYEMQLNALQLKSEECEQLREKIINLELMISNNSEEKIQFEEKLDKLKQVLNKVENEKRNLQEELSKSESRATKLELQRMSLEGDLQRLQMMFQEKDANIHKLQERNDTQNRTMTTLEERCTSLKSMVEQLNLALEKASTTENELKNEINSMQHNIMELTTTLQTSNEKNKQLQKQISNAENERRILSERIESMQQSLNDLKHTNQTLTDQITRLQNELANNEVQRCALESQLRIVAYPTQEENINKDEELLRQLQIAQRERSEMRGKMEALNDKMKLLEADKRNLERQLSLFKSTNRSKSYERYEKAHTELLGTSFDIDHYEQENRELRLKVRRLETQLAEKEAELIRLKSSYTHTHSVFDFNRDRTGEIERLRAAQLQAEKLLEAREQSHRQQVLRLENQIQLLREQLNQEIKRRQLYVLRSSRAGREMQQLRQALGDSLRTVAQDPSLDAVLLEHEARKLDSTLTSTTSLPPSLALPPPPSYDRSSTPAQLK

Target ORF information:

RefSeq Version	XM_016917087.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera rootletin (LOC552019), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016917087.1

ORF Insert Sequence:

ATGAGAAGAA TGCCATTGCC TAAAAATATT TCTCAATTAT CAGCAAGAGG ACAGATGGAG 
AGGCGGCGAC CACCGTTTCT CAGAGGCCCT AGTAGAATGA GAGAGAAAAT AGAAAGAGGC 
ATAGGCAGTA AAATGGATTC TACAGGAAGT ACACTAAGTG GAACTGGAGA TTGTTTAAGT 
GAAGAAGATC TTTCACCAGA TGCATTGGTT AGACAAAACT ATGAATTACG TCATCGTCTT 
GAAGAAGAAG CAGCCAATTA TAAAAGACGT TTGGATACAT ATAGACAAGC ACAACAACAT 
CAGGCTGCTC TTGTTTCACG TTTACAAGCC AAAGTATTGC AATATAAACA AAAATGTTCA 
GAGTTAGAAA ATCAAATGGC AGAATCTATA GTATATGATT CTAATAAAGT TGTAAGTTCT 
GCACCTCCAA CAACTTCTGT TTTAGATGCA GCTCATCAAA CACTAAGAGA AATACGTGAA 
GAACAAATAT ATGATTTAGA TACTGCTCTA AAAAAACTAA GTGAAGAACG TAAAAGATGT 
GAAAAATTGC TGCAATTAAA TACAACATTG AAAGATCAGT TAGAAGAATC TCATCAAACA 
AATGAAGCAC TTACAAATGA TCTACAAAAA TTAAGTAATG ATTGGGATAT TTTAAGAGAA 
GAATTAGCTA TCAAAGAAGA AGAGTGGAGA GAAGAAGAGC AAGCTTTTAA TGATTATTAT 
ACTTCAGAAC ATAACAGATT ATTAATTCTT TGGCGCGATG TTGTTTCTGT AAAACGATTA 
TTTGCAGATA TAAAATCTAG TACAGAAAGG GATCTATTAA AAATGAAAAA TAACATTATT 
TCTACTTTTA ATGATGTTTC ATCAGCTTGT AATAATACAG GATTTGCTAT GAGAATGCAA 
GCAGCTATGC ATCCAATGAT ATCTCAACAA GTTCAACAGG CACAAGAACA GGTAACAACT 
GATTTAAAAA CAGAATTAAT AACACTTAAA CAACAGTATG ATGTAGCTCA AAATGAAATT 
CGTATAAAGG AAGAAAAAAT CAATCAACTT ATTCGTGATG TTCACAGTTT GGAAGAAAGA 
TGTGGAGAAG CAGAAACAGA AATTCATCGC AATACACGTT TGCAAGATGA TATAGAAATT 
TTACAATCTG CATTGAGAGA TATAGCACAT GCTGTAATTC AAGATGCAGA AAGTCGCGAA 
ATTGAATCAA CTCAAGCACC TCCTCATCTT CATTTATCAC CTACTGGACC AATTCCTCAA 
AAATCACCAA AAAGAGGTAC AAGAAGCAAC ACCATTCCAG CTTTTGCTGA AAGTACTATA 
AGTGCTGTAC AAGCAGCTCT CCATAAATAT CAATTGACTA TACATGAACT TCAGGTGAAA 
TTGCAAACTA ATAAAGAACA ACTACAAAAT ACTCGCAAAC AATGCGAAAA TGCGGAAGAG 
AATGTTAAAA CTCTAGAGAA AAAAGCAGAA GAACTGATTA TTGAATTAGA TGCAGTTCGA 
TTGCACTGTT CACAACTTAA TCAAGAAAAA GATATGTTAC AAAAAGGACT TGATACTATA 
AGAATAGAAA AAAATACTCT TGAAAAAACC AGGATAGAAC TTAATAATAT GATGGAGAAT 
TTAAACAATG ATTGCGAAAA ATTGCAAAAA GCTAACAACA AATTGCAAAA AATTTGCGAC 
AATTTAGAAG ATGAAAAATT ATATCTTCAA AGTGAACTCA ATAGATTATC TAAAGATGTA 
GAATTGAGGG AATTAAATTT GCGTTCAGAA GAAGATAGGT GCAGTAAAAT GAGAGAGGAA 
TTATTAACTC TACGTGAAGA ATTGAATAAA ACATATCTCA GTAAAGATAT GTTAGAACAA 
CAAAAGTTAG AAACAGATGG ATTAATTTCA CAAATTGAAA AAAGCAAAGG TGATTTAGAA 
TTAGAATTGG AACGCGTATT ATTAGAAAAA TCAGACGTGC AAGAAGTTTT AATGAAACTA 
GAAACAGTTT GCAGCAATCA TGAACAAGAG AAACAACGAT TACAAGAAGA ATTAAAAAAG 
GTAACAGAAG AAAAAAATAA GTTAGCAAAT CAATGTACGG ATCAACAAGG TGATCTGGGT 
TCCTTAAGAA AAGAATTACT TCAAGCAGAA CAAACTAGAC TTGATTTAGA ATCAGAAAAA 
GTAACTTTGA ATGAAAAAGT GAAATTTCTG GAGATCGAAA AGGAGAAGAT TGAAATAGAA 
TTGGCACAAG TTACGCGCGA ACGTGGAGAT TTCAATAATC AATTATCAGT TTTAGCACGA 
AAGAAAGAAA CATTAAACGA AGAATTGATG AGACTCAAAC AAAGATTAGA ACAGGCAAAC 
GAAATGAATG GAAGAATAAA TCGGAATTTA GAAGATCTTG TAAAAGATAA TGAAGAAAAA 
CAAGTTCTTT TAGAAACTAA TGAAAAGGAA GTACAACGAT TACAAGAGCA ACTTGCTTCA 
ATGCGTAGTG AGAAAGAGAC ATTAGAAGGT GTTTTATTTG ATACACAAAC TAGTCTAGAA 
AATATGCATG TGAAAAAGAC TCAATTAGAA AAGGAACAGA AAGAACTTTT AATAAAACAA 
GAAAGCTTAA AAGGACAAGT AGAAAGATTA ATGAAAGATT TGGAGAATAG CGAAAAACGA 
ATTCATGAAG TAAAGCAAAC TTTAACGCAA CAAAGTGGCG ATCAAGAAGC CGAATTTCAA 
CAAATTATTT CTAACGTGAA AAAACATAGT GAAGAAAACA TAAAAAAATT AAATGAAGAA 
AAAGAACAAA TAAAGATAAA TTTAGAAAAA CGACTTCAGC AATCTCTCTT GCAAGTTACT 
GGAGAAAAAG ATAATGAAAT AAATCAATTG CAGCAAAGAA TAGATGAAAT GCAACAACAT 
ATAGAAAATT TGTGTCAGCA ACATGAAGAA GTTCTTCTTA GAGCAGAAAA TGATAAACAG 
CAAGCTCTTC TTATAGCTCA TCATGATCAG CAAGCATTAA TGGAAAAACT TGAAACTGTT 
TTGCATGAAA TGGAAGAAGA GAAAAATAAT GTAGAACGAG TCAAACGAGA AGCTGCAGTA 
AAAACTGAAC AAGAGCGTAA TAATATAAAT CAATTACGTG ATGAATTAAA TCGTCTTAAA 
ACAAAATTAG ATGAAACTAG ATTAAAAACT GATGAAGAAA AAATAAAACT TGATCTAAAA 
ATAGAAGAAC TCTGGAAAGA ACGTGAATTA GCACAAAGAG AATCCGAAGA ATTACAAGTT 
CAATTACATA TGACAGAAGA TAAAGTTGAT AGTTTACAAA ATCAATTGCA TGAAACTATT 
AGAAAACTTA AAGATGCTGA AAATCTTAAC GAAACATTAC GCAAAGAATT AGTAGATATT 
AGAAGACAAT TAGGTGATTC CACATATGAA AAAGAAAAGT ATAATAGTAG TAATAAAGAA 
TTACGCGAGC ATGTAAAACG CATTGAAAGC GAAAAAAGAG AACAAAATAG AATATTAGAA 
GAATCATATC AAAAAATTTC AGCACTGGAA GATATGAAAG TAAATGTAGA TGCAGAGAGA 
TCTCGTCTTC AAGCACAAAT TCGTGATATG GAGAAAGAAA TGTTGCAATT ACAAAAACAA 
CTTCATTTTA CTCAAGATGA ATTACAAAAA TCACATCAAA GTAATGCTCA AGCACAAAAT 
GATGAAAAAG AATTGCAAGC TCGACTAACT AACGAAACAG AAGAAAGAGA ACGTTTACAA 
TTGCAGTTAC ATCAAGTAAA AAAACAGTTA ATGGATGTTG ACAATAGTCT AAAAGTAACA 
AGACAAGAAC TTGGAAGATT GCGGTCACGT GCAGATGAAG AGAATGAACG GTGGAGAGTT 
AGAGAACAAG AACTAGTAGT TCGTCTTGAG GATAGTCGAT GTCGTGAAAG AAAACTCGAG 
GATCAAAAAC ATAACTTAGA AGTTTGTTTA GCTGATGCTT CTCAACAAAT TCAAGAACTG 
AAGGCACGTC TTGGAGGTTC CGAAGGACGA GTTCGAGCTT TAGATGCGCA ATTATCGCAA 
TTAGAAATAA CAAAAAAAGA AGTAGAACAA AAATTAAGTA GCGTGGGATC GACTTTACGT 
CGAATTGCAG GTATTCAAAT GGATGGAAGT GTTAATATAC CCTTTAAATT ATTGAGTCCA 
TCAAGAAGGT GGAGTCCAGC AAGAGCTCAA GATCATATTG ATTCTAGTAG AGATGTGATT 
CTTGATGTTG ATCCTGAGAT TATTAGAAAA GGTGTACGAT CTCTTATGCA ACAAGTAGCA 
CAAATCGAAC GTGAAAGAGA TGATTATAAA ACTGAATTAT GTAATTTGAA AAAACAATTG 
GATGAAAATC AAGAGATTCA AAATAGATCT GATATACAAA TAAACACTTT ATTAACAAAT 
ATAAGAATGC TTCAGGATGA AAAGAATTCA TTGGAGGTAA AACTTTCTCA AAAACAAAGT 
GGTTATGAAA TGCAGTTAAA TGCTTTACAA CTAAAGTCAG AAGAATGCGA GCAATTACGC 
GAGAAGATTA TCAATCTTGA ATTAATGATT AGTAATAATT CTGAAGAAAA AATACAATTT 
GAGGAAAAAC TAGATAAATT AAAACAAGTT TTAAACAAAG TAGAAAATGA AAAACGTAAT 
TTACAAGAAG AACTTAGTAA AAGCGAATCC CGCGCTACGA AATTAGAATT ACAAAGAATG 
TCATTAGAAG GTGACCTTCA AAGATTGCAA ATGATGTTCC AAGAAAAAGA TGCAAATATT 
CATAAATTAC AAGAACGAAA TGATACACAA AATCGAACAA TGACAACTTT AGAAGAACGT 
TGTACTTCAT TGAAATCTAT GGTGGAACAA TTAAATCTTG CATTGGAAAA AGCATCTACT 
ACAGAAAATG AATTAAAGAA TGAAATTAAT TCAATGCAAC ATAATATTAT GGAATTAACA 
ACTACTTTGC AAACTTCTAA TGAAAAAAAT AAACAGTTAC AAAAACAAAT TTCAAATGCT 
GAAAATGAAC GTAGAATCTT ATCTGAACGT ATAGAATCAA TGCAACAATC TTTAAATGAT 
CTCAAACATA CAAATCAAAC ATTAACAGAT CAAATTACAC GTCTTCAAAA TGAATTGGCA 
AATAATGAAG TACAACGTTG TGCCTTAGAA TCTCAATTAA GAATAGTTGC CTATCCAACA 
CAAGAAGAAA ATATAAATAA GGATGAAGAA TTATTACGAC AGTTACAAAT AGCACAAAGA 
GAGAGAAGTG AAATGAGAGG AAAAATGGAA GCACTTAATG ATAAAATGAA ATTATTGGAA 
GCTGATAAAC GTAATTTAGA ACGACAACTA TCGTTATTTA AATCAACTAA TCGTAGCAAA 
AGTTATGAAC GTTATGAGAA AGCACATACA GAATTATTAG GCACAAGTTT CGATATAGAT 
CATTATGAAC AAGAAAATAG AGAATTAAGA TTGAAAGTTC GTAGATTAGA AACACAACTT 
GCAGAGAAGG AAGCTGAACT TATAAGATTG AAATCAAGTT ACACTCATAC ACATTCCGTA 
TTTGATTTTA ATCGAGATAG AACGGGCGAA ATTGAAAGAC TTAGAGCAGC TCAATTACAA 
GCTGAAAAAT TGTTAGAAGC AAGAGAACAA AGTCATCGTC AACAAGTTTT GCGTTTGGAA 
AATCAGATAC AATTGTTACG CGAGCAACTT AATCAAGAAA TAAAACGTCG ACAATTATAT 
GTTTTACGAA GTTCAAGGGC AGGTAGAGAA ATGCAACAAT TAAGACAAGC CTTAGGCGAT 
TCTTTAAGAA CTGTAGCTCA AGATCCATCA TTAGACGCAG TATTATTAGA ACATGAAGCT 
CGAAAATTGG ATTCTACTTT GACAAGTACT ACCAGTTTAC CACCATCATT AGCTTTACCT 
CCGCCACCGT CTTATGATAG ATCTTCCACA CCAGCACAAC TCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552019 ( XM_016917088.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016772577.1 Apis mellifera rootletin (LOC552019), transcript variant X3, mRNA.

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CloneID	OHd20602
Clone ID Related Accession (Same CDS sequence)	XM_016917088.1
Accession Version	XM_016917088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	rootletin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007085.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGAA TGCCATTGCC TAAAAATATT TCTCAATTAT CAGCAAGAGG ACAGATGGAG 
AGGCGGCGAC CACCGTTTCT CAGAGGCCCT AGTAGAATGA GAGAGAAAAT AGAAAGAGGC 
ATAGGCAGTA AAATGGATTC TACAGGAAGT ACACTAAGTG GAACTGGAGA TTGTTTAAGT 
GAAGAAGATC TTTCACCAGA TGCATTGGTT AGACAAAACT ATGAATTACG TCATCGTCTT 
GAAGAAGAAG CAGCCAATTA TAAAAGACGT TTGGATACAT ATAGACAAGC ACAACAACAT 
CAGGCTGCTC TTGTTTCACG TTTACAAGCC AAAGTATTGC AATATAAACA AAAATGTTCA 
GAGTTAGAAA ATCAAATGGC AGAATCTATA GTATATGATT CTAATAAAGT TGTAAGTTCT 
GCACCTCCAA CAACTTCTGT TTTAGATGCA GCTCATCAAA CACTAAGAGA AATACGTGAA 
GAACAAATAT ATGATTTAGA TACTGCTCTA AAAAAACTAA GTGAAGAACG TAAAAGATGT 
GAAAAATTGC TGCAATTAAA TACAACATTG AAAGATCAGT TAGAAGAATC TCATCAAACA 
AATGAAGCAC TTACAAATGA TCTACAAAAA TTAAGTAATG ATTGGGATAT TTTAAGAGAA 
GAATTAGCTA TCAAAGAAGA AGAGTGGAGA GAAGAAGAGC AAGCTTTTAA TGATTATTAT 
ACTTCAGAAC ATAACAGATT ATTAATTCTT TGGCGCGATG TTGTTTCTGT AAAACGATTA 
TTTGCAGATA TAAAATCTAG TACAGAAAGG GATCTATTAA AAATGAAAAA TAACATTATT 
TCTACTTTTA ATGATGTTTC ATCAGCTTGT AATAATACAG GATTTGCTAT GAGAATGCAA 
GCAGCTATGC ATCCAATGAT ATCTCAACAA GTTCAACAGG CACAAGAACA GGTAACAACT 
GATTTAAAAA CAGAATTAAT AACACTTAAA CAACAGTATG ATGTAGCTCA AAATGAAATT 
CGTATAAAGG AAGAAAAAAT CAATCAACTT ATTCGTGATG TTCACAGTTT GGAAGAAAGA 
TGTGGAGAAG CAGAAACAGA AATTCATCGC AATACACGTT TGCAAGATGA TATAGAAATT 
TTACAATCTG CATTGAGAGA TATAGCACAT GCTGTAATTC AAGATGCAGA AAGTCGCGAA 
ATTGAATCAA CTCAAGCACC TCCTCATCTT CATTTATCAC CTACTGGACC AATTCCTCAA 
AAATCACCAA AAAGAGGTAC AAGAAGCAAC ACCATTCCAG CTTTTGCTGA AAGTACTATA 
AGTGCTGTAC AAGCAGCTCT CCATAAATAT CAATTGACTA TACATGAACT TCAGGTGAAA 
TTGCAAACTA ATAAAGAACA ACTACAAAAT ACTCGCAAAC AATGCGAAAA TGCGGAAGAG 
AATGTTAAAA CTCTAGAGAA AAAAGCAGAA GAACTGATTA TTGAATTAGA TGCAGTTCGA 
TTGCACTGTT CACAACTTAA TCAAGAAAAA GATATGTTAC AAAAAGGACT TGATACTATA 
AGAATAGAAA AAAATACTCT TGAAAAAACC AGGATAGAAC TTAATAATAT GATGGAGAAT 
TTAAACAATG ATTGCGAAAA ATTGCAAAAA GCTAACAACA AATTGCAAAA AATTTGCGAC 
AATTTAGAAG ATGAAAAATT ATATCTTCAA AGTGAACTCA ATAGATTATC TAAAGATGTA 
GAATTGAGGG AATTAAATTT GCGTTCAGAA GAAGATAGGT GCAGTAAAAT GAGAGAGGAA 
TTATTAACTC TACGTGAAGA ATTGAATAAA ACATATCTCA GTAAAGATAT GTTAGAACAA 
CAAAAGTTAG AAACAGATGG ATTAATTTCA CAAATTGAAA AAAGCAAAGG TGATTTAGAA 
TTAGAATTGG AACGCGTATT ATTAGAAAAA TCAGACGTGC AAGAAGTTTT AATGAAACTA 
GAAACAGTTT GCAGCAATCA TGAACAAGAG AAACAACGAT TACAAGAAGA ATTAAAAAAG 
GTAACAGAAG AAAAAAATAA GTTAGCAAAT CAATGTACGG ATCAACAAGG TGATCTGGGT 
TCCTTAAGAA AAGAATTACT TCAAGCAGAA CAAACTAGAC TTGATTTAGA ATCAGAAAAA 
GTAACTTTGA ATGAAAAAGT GAAATTTCTG GAGATCGAAA AGGAGAAGAT TGAAATAGAA 
TTGGCACAAG TTACGCGCGA ACGTGGAGAT TTCAATAATC AATTATCAGT TTTAGCACGA 
AAGAAAGAAA CATTAAACGA AGAATTGATG AGACTCAAAC AAAGATTAGA ACAGGCAAAC 
GAAATGAATG GAAGAATAAA TCGGAATTTA GAAGATCTTG TAAAAGATAA TGAAGAAAAA 
CAAGTTCTTT TAGAAACTAA TGAAAAGGAA GTACAACGAT TACAAGAGCA ACTTGCTTCA 
ATGCGTAGTG AGAAAGAGAC ATTAGAAGGT GTTTTATTTG ATACACAAAC TAGTCTAGAA 
AATATGCATG TGAAAAAGAC TCAATTAGAA AAGGAACAGA AAGAACTTTT AATAAAACAA 
GAAAGCTTAA AAGGACAAGT AGAAAGATTA ATGAAAGATT TGGAGAATAG CGAAAAACGA 
ATTCATGAAG TAAAGCAAAC TTTAACGCAA CAAAGTGGCG ATCAAGAAGC CGAATTTCAA 
CAAATTATTT CTAACGTGAA AAAACATAGT GAAGAAAACA TAAAAAAATT AAATGAAGAA 
AAAGAACAAA TAAAGATAAA TTTAGAAAAA CGACTTCAGC AATCTCTCTT GCAAGTTACT 
GGAGAAAAAG ATAATGAAAT AAATCAATTG CAGCAAAGAA TAGATGAAAT GCAACAACAT 
ATAGAAAATT TGTGTCAGCA ACATGAAGAA GTTCTTCTTA GAGCAGAAAA TGATAAACAG 
CAAGCTCTTC TTATAGCTCA TCATGATCAG CAAGCATTAA TGGAAAAACT TGAAACTGTT 
TTGCATGAAA TGGAAGAAGA GAAAAATAAT GTAGAACGAG TCAAACGAGA AGCTGCAGTA 
AAAACTGAAC AAGAGCGTAA TAATATAAAT CAATTACGTG ATGAATTAAA TCGTCTTAAA 
ACAAAATTAG ATGAAACTAG ATTAAAAACT GATGAAGAAA AAATAAAACT TGATCTAAAA 
ATAGAAGAAC TCTGGAAAGA ACGTGAATTA GCACAAAGAG AATCCGAAGA ATTACAAGTT 
CAATTACATA TGACAGAAGA TAAAGTTGAT AGTTTACAAA ATCAATTGCA TGAAACTATT 
AGAAAACTTA AAGATGCTGA AAATCTTAAC GAAACATTAC GCAAAGAATT AGTAGATATT 
AGAAGACAAT TAGGTGATTC CACATATGAA AAAGAAAAGT ATAATAGTAG TAATAAAGAA 
TTACGCGAGC ATGTAAAACG CATTGAAAGC GAAAAAAGAG AACAAAATAG AATATTAGAA 
GAATCATATC AAAAAATTTC AGCACTGGAA GATATGAAAG TAAATGTAGA TGCAGAGAGA 
TCTCGTCTTC AAGCACAAAT TCGTGATATG GAGAAAGAAA TGTTGCAATT ACAAAAACAA 
CTTCATTTTA CTCAAGATGA ATTACAAAAA TCACATCAAA GTAATGCTCA AGCACAAAAT 
GATGAAAAAG AATTGCAAGC TCGACTAACT AACGAAACAG AAGAAAGAGA ACGTTTACAA 
TTGCAGTTAC ATCAAGTAAA AAAACAGTTA ATGGATGTTG ACAATAGTCT AAAAGTAACA 
AGACAAGAAC TTGGAAGATT GCGGTCACGT GCAGATGAAG AGAATGAACG GTGGAGAGTT 
AGAGAACAAG AACTAGTAGT TCGTCTTGAG GATAGTCGAT GTCGTGAAAG AAAACTCGAG 
GATCAAAAAC ATAACTTAGA AGTTTGTTTA GCTGATGCTT CTCAACAAAT TCAAGAACTG 
AAGGCACGTC TTGGAGGTTC CGAAGGACGA GTTCGAGCTT TAGATGCGCA ATTATCGCAA 
TTAGAAATAA CAAAAAAAGA AGTAGAACAA AAATTAAGTA GCGTGGGATC GACTTTACGT 
CGAATTGCAG GTATTCAAAT GGATGGAAGT GTTAATATAC CCTTTAAATT ATTGAGTCCA 
TCAAGAAGGT GGAGTCCAGC AAGAGCTCAA GATCATATTG ATTCTAGTAG AGATGTGATT 
CTTGATGTTG ATCCTGAGAT TATTAGAAAA GGTGTACGAT CTCTTATGCA ACAAGTAGCA 
CAAATCGAAC GTGAAAGAGA TGATTATAAA ACTGAATTAT GTAATTTGAA AAAACAATTG 
GATGAAAATC AAGAGATTCA AAATAGATCT GATATACAAA TAAACACTTT ATTAACAAAT 
ATAAGAATGC TTCAGGATGA AAAGAATTCA TTGGAGGTAA AACTTTCTCA AAAACAAAGT 
GGTTATGAAA TGCAGTCAGA AGAATGCGAG CAATTACGCG AGAAGATTAT CAATCTTGAA 
TTAATGATTA GTAATAATTC TGAAGAAAAA ATACAATTTG AGGAAAAACT AGATAAATTA 
AAACAAGTTT TAAACAAAGT AGAAAATGAA AAACGTAATT TACAAGAAGA ACTTAGTAAA 
AGCGAATCCC GCGCTACGAA ATTAGAATTA CAAAGAATGT CATTAGAAGG TGACCTTCAA 
AGATTGCAAA TGATGTTCCA AGAAAAAGAT GCAAATATTC ATAAATTACA AGAACGAAAT 
GATACACAAA ATCGAACAAT GACAACTTTA GAAGAACGTT GTACTTCATT GAAATCTATG 
GTGGAACAAT TAAATCTTGC ATTGGAAAAA GCATCTACTA CAGAAAATGA ATTAAAGAAT 
GAAATTAATT CAATGCAACA TAATATTATG GAATTAACAA CTACTTTGCA AACTTCTAAT 
GAAAAAAATA AACAGTTACA AAAACAAATT TCAAATGCTG AAAATGAACG TAGAATCTTA 
TCTGAACGTA TAGAATCAAT GCAACAATCT TTAAATGATC TCAAACATAC AAATCAAACA 
TTAACAGATC AAATTACACG TCTTCAAAAT GAATTGGCAA ATAATGAAGT ACAACGTTGT 
GCCTTAGAAT CTCAATTAAG AATAGTTGCC TATCCAACAC AAGAAGAAAA TATAAATAAG 
GATGAAGAAT TATTACGACA GTTACAAATA GCACAAAGAG AGAGAAGTGA AATGAGAGGA 
AAAATGGAAG CACTTAATGA TAAAATGAAA TTATTGGAAG CTGATAAACG TAATTTAGAA 
CGACAACTAT CGTTATTTAA ATCAACTAAT CGTAGCAAAA GTTATGAACG TTATGAGAAA 
GCACATACAG AATTATTAGG CACAAGTTTC GATATAGATC ATTATGAACA AGAAAATAGA 
GAATTAAGAT TGAAAGTTCG TAGATTAGAA ACACAACTTG CAGAGAAGGA AGCTGAACTT 
ATAAGATTGA AATCAAGTTA CACTCATACA CATTCCGTAT TTGATTTTAA TCGAGATAGA 
ACGGGCGAAA TTGAAAGACT TAGAGCAGCT CAATTACAAG CTGAAAAATT GTTAGAAGCA 
AGAGAACAAA GTCATCGTCA ACAAGTTTTG CGTTTGGAAA ATCAGATACA ATTGTTACGC 
GAGCAACTTA ATCAAGAAAT AAAACGTCGA CAATTATATG TTTTACGAAG TTCAAGGGCA 
GGTAGAGAAA TGCAACAATT AAGACAAGCC TTAGGCGATT CTTTAAGAAC TGTAGCTCAA 
GATCCATCAT TAGACGCAGT ATTATTAGAA CATGAAGCTC GAAAATTGGA TTCTACTTTG 
ACAAGTACTA CCAGTTTACC ACCATCATTA GCTTTACCTC CGCCACCGTC TTATGATAGA 
TCTTCCACAC CAGCACAACT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016772577.1
CDS	176..6082
Translation	MRRMPLPKNISQLSARGQMERRRPPFLRGPSRMREKIERGIGSKMDSTGSTLSGTGDCLSEEDLSPDALVRQNYELRHRLEEEAANYKRRLDTYRQAQQHQAALVSRLQAKVLQYKQKCSELENQMAESIVYDSNKVVSSAPPTTSVLDAAHQTLREIREEQIYDLDTALKKLSEERKRCEKLLQLNTTLKDQLEESHQTNEALTNDLQKLSNDWDILREELAIKEEEWREEEQAFNDYYTSEHNRLLILWRDVVSVKRLFADIKSSTERDLLKMKNNIISTFNDVSSACNNTGFAMRMQAAMHPMISQQVQQAQEQVTTDLKTELITLKQQYDVAQNEIRIKEEKINQLIRDVHSLEERCGEAETEIHRNTRLQDDIEILQSALRDIAHAVIQDAESREIESTQAPPHLHLSPTGPIPQKSPKRGTRSNTIPAFAESTISAVQAALHKYQLTIHELQVKLQTNKEQLQNTRKQCENAEENVKTLEKKAEELIIELDAVRLHCSQLNQEKDMLQKGLDTIRIEKNTLEKTRIELNNMMENLNNDCEKLQKANNKLQKICDNLEDEKLYLQSELNRLSKDVELRELNLRSEEDRCSKMREELLTLREELNKTYLSKDMLEQQKLETDGLISQIEKSKGDLELELERVLLEKSDVQEVLMKLETVCSNHEQEKQRLQEELKKVTEEKNKLANQCTDQQGDLGSLRKELLQAEQTRLDLESEKVTLNEKVKFLEIEKEKIEIELAQVTRERGDFNNQLSVLARKKETLNEELMRLKQRLEQANEMNGRINRNLEDLVKDNEEKQVLLETNEKEVQRLQEQLASMRSEKETLEGVLFDTQTSLENMHVKKTQLEKEQKELLIKQESLKGQVERLMKDLENSEKRIHEVKQTLTQQSGDQEAEFQQIISNVKKHSEENIKKLNEEKEQIKINLEKRLQQSLLQVTGEKDNEINQLQQRIDEMQQHIENLCQQHEEVLLRAENDKQQALLIAHHDQQALMEKLETVLHEMEEEKNNVERVKREAAVKTEQERNNINQLRDELNRLKTKLDETRLKTDEEKIKLDLKIEELWKERELAQRESEELQVQLHMTEDKVDSLQNQLHETIRKLKDAENLNETLRKELVDIRRQLGDSTYEKEKYNSSNKELREHVKRIESEKREQNRILEESYQKISALEDMKVNVDAERSRLQAQIRDMEKEMLQLQKQLHFTQDELQKSHQSNAQAQNDEKELQARLTNETEERERLQLQLHQVKKQLMDVDNSLKVTRQELGRLRSRADEENERWRVREQELVVRLEDSRCRERKLEDQKHNLEVCLADASQQIQELKARLGGSEGRVRALDAQLSQLEITKKEVEQKLSSVGSTLRRIAGIQMDGSVNIPFKLLSPSRRWSPARAQDHIDSSRDVILDVDPEIIRKGVRSLMQQVAQIERERDDYKTELCNLKKQLDENQEIQNRSDIQINTLLTNIRMLQDEKNSLEVKLSQKQSGYEMQSEECEQLREKIINLELMISNNSEEKIQFEEKLDKLKQVLNKVENEKRNLQEELSKSESRATKLELQRMSLEGDLQRLQMMFQEKDANIHKLQERNDTQNRTMTTLEERCTSLKSMVEQLNLALEKASTTENELKNEINSMQHNIMELTTTLQTSNEKNKQLQKQISNAENERRILSERIESMQQSLNDLKHTNQTLTDQITRLQNELANNEVQRCALESQLRIVAYPTQEENINKDEELLRQLQIAQRERSEMRGKMEALNDKMKLLEADKRNLERQLSLFKSTNRSKSYERYEKAHTELLGTSFDIDHYEQENRELRLKVRRLETQLAEKEAELIRLKSSYTHTHSVFDFNRDRTGEIERLRAAQLQAEKLLEAREQSHRQQVLRLENQIQLLREQLNQEIKRRQLYVLRSSRAGREMQQLRQALGDSLRTVAQDPSLDAVLLEHEARKLDSTLTSTTSLPPSLALPPPPSYDRSSTPAQLK

Target ORF information:

RefSeq Version	XM_016917088.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera rootletin (LOC552019), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016917088.1

ORF Insert Sequence:

ATGAGAAGAA TGCCATTGCC TAAAAATATT TCTCAATTAT CAGCAAGAGG ACAGATGGAG 
AGGCGGCGAC CACCGTTTCT CAGAGGCCCT AGTAGAATGA GAGAGAAAAT AGAAAGAGGC 
ATAGGCAGTA AAATGGATTC TACAGGAAGT ACACTAAGTG GAACTGGAGA TTGTTTAAGT 
GAAGAAGATC TTTCACCAGA TGCATTGGTT AGACAAAACT ATGAATTACG TCATCGTCTT 
GAAGAAGAAG CAGCCAATTA TAAAAGACGT TTGGATACAT ATAGACAAGC ACAACAACAT 
CAGGCTGCTC TTGTTTCACG TTTACAAGCC AAAGTATTGC AATATAAACA AAAATGTTCA 
GAGTTAGAAA ATCAAATGGC AGAATCTATA GTATATGATT CTAATAAAGT TGTAAGTTCT 
GCACCTCCAA CAACTTCTGT TTTAGATGCA GCTCATCAAA CACTAAGAGA AATACGTGAA 
GAACAAATAT ATGATTTAGA TACTGCTCTA AAAAAACTAA GTGAAGAACG TAAAAGATGT 
GAAAAATTGC TGCAATTAAA TACAACATTG AAAGATCAGT TAGAAGAATC TCATCAAACA 
AATGAAGCAC TTACAAATGA TCTACAAAAA TTAAGTAATG ATTGGGATAT TTTAAGAGAA 
GAATTAGCTA TCAAAGAAGA AGAGTGGAGA GAAGAAGAGC AAGCTTTTAA TGATTATTAT 
ACTTCAGAAC ATAACAGATT ATTAATTCTT TGGCGCGATG TTGTTTCTGT AAAACGATTA 
TTTGCAGATA TAAAATCTAG TACAGAAAGG GATCTATTAA AAATGAAAAA TAACATTATT 
TCTACTTTTA ATGATGTTTC ATCAGCTTGT AATAATACAG GATTTGCTAT GAGAATGCAA 
GCAGCTATGC ATCCAATGAT ATCTCAACAA GTTCAACAGG CACAAGAACA GGTAACAACT 
GATTTAAAAA CAGAATTAAT AACACTTAAA CAACAGTATG ATGTAGCTCA AAATGAAATT 
CGTATAAAGG AAGAAAAAAT CAATCAACTT ATTCGTGATG TTCACAGTTT GGAAGAAAGA 
TGTGGAGAAG CAGAAACAGA AATTCATCGC AATACACGTT TGCAAGATGA TATAGAAATT 
TTACAATCTG CATTGAGAGA TATAGCACAT GCTGTAATTC AAGATGCAGA AAGTCGCGAA 
ATTGAATCAA CTCAAGCACC TCCTCATCTT CATTTATCAC CTACTGGACC AATTCCTCAA 
AAATCACCAA AAAGAGGTAC AAGAAGCAAC ACCATTCCAG CTTTTGCTGA AAGTACTATA 
AGTGCTGTAC AAGCAGCTCT CCATAAATAT CAATTGACTA TACATGAACT TCAGGTGAAA 
TTGCAAACTA ATAAAGAACA ACTACAAAAT ACTCGCAAAC AATGCGAAAA TGCGGAAGAG 
AATGTTAAAA CTCTAGAGAA AAAAGCAGAA GAACTGATTA TTGAATTAGA TGCAGTTCGA 
TTGCACTGTT CACAACTTAA TCAAGAAAAA GATATGTTAC AAAAAGGACT TGATACTATA 
AGAATAGAAA AAAATACTCT TGAAAAAACC AGGATAGAAC TTAATAATAT GATGGAGAAT 
TTAAACAATG ATTGCGAAAA ATTGCAAAAA GCTAACAACA AATTGCAAAA AATTTGCGAC 
AATTTAGAAG ATGAAAAATT ATATCTTCAA AGTGAACTCA ATAGATTATC TAAAGATGTA 
GAATTGAGGG AATTAAATTT GCGTTCAGAA GAAGATAGGT GCAGTAAAAT GAGAGAGGAA 
TTATTAACTC TACGTGAAGA ATTGAATAAA ACATATCTCA GTAAAGATAT GTTAGAACAA 
CAAAAGTTAG AAACAGATGG ATTAATTTCA CAAATTGAAA AAAGCAAAGG TGATTTAGAA 
TTAGAATTGG AACGCGTATT ATTAGAAAAA TCAGACGTGC AAGAAGTTTT AATGAAACTA 
GAAACAGTTT GCAGCAATCA TGAACAAGAG AAACAACGAT TACAAGAAGA ATTAAAAAAG 
GTAACAGAAG AAAAAAATAA GTTAGCAAAT CAATGTACGG ATCAACAAGG TGATCTGGGT 
TCCTTAAGAA AAGAATTACT TCAAGCAGAA CAAACTAGAC TTGATTTAGA ATCAGAAAAA 
GTAACTTTGA ATGAAAAAGT GAAATTTCTG GAGATCGAAA AGGAGAAGAT TGAAATAGAA 
TTGGCACAAG TTACGCGCGA ACGTGGAGAT TTCAATAATC AATTATCAGT TTTAGCACGA 
AAGAAAGAAA CATTAAACGA AGAATTGATG AGACTCAAAC AAAGATTAGA ACAGGCAAAC 
GAAATGAATG GAAGAATAAA TCGGAATTTA GAAGATCTTG TAAAAGATAA TGAAGAAAAA 
CAAGTTCTTT TAGAAACTAA TGAAAAGGAA GTACAACGAT TACAAGAGCA ACTTGCTTCA 
ATGCGTAGTG AGAAAGAGAC ATTAGAAGGT GTTTTATTTG ATACACAAAC TAGTCTAGAA 
AATATGCATG TGAAAAAGAC TCAATTAGAA AAGGAACAGA AAGAACTTTT AATAAAACAA 
GAAAGCTTAA AAGGACAAGT AGAAAGATTA ATGAAAGATT TGGAGAATAG CGAAAAACGA 
ATTCATGAAG TAAAGCAAAC TTTAACGCAA CAAAGTGGCG ATCAAGAAGC CGAATTTCAA 
CAAATTATTT CTAACGTGAA AAAACATAGT GAAGAAAACA TAAAAAAATT AAATGAAGAA 
AAAGAACAAA TAAAGATAAA TTTAGAAAAA CGACTTCAGC AATCTCTCTT GCAAGTTACT 
GGAGAAAAAG ATAATGAAAT AAATCAATTG CAGCAAAGAA TAGATGAAAT GCAACAACAT 
ATAGAAAATT TGTGTCAGCA ACATGAAGAA GTTCTTCTTA GAGCAGAAAA TGATAAACAG 
CAAGCTCTTC TTATAGCTCA TCATGATCAG CAAGCATTAA TGGAAAAACT TGAAACTGTT 
TTGCATGAAA TGGAAGAAGA GAAAAATAAT GTAGAACGAG TCAAACGAGA AGCTGCAGTA 
AAAACTGAAC AAGAGCGTAA TAATATAAAT CAATTACGTG ATGAATTAAA TCGTCTTAAA 
ACAAAATTAG ATGAAACTAG ATTAAAAACT GATGAAGAAA AAATAAAACT TGATCTAAAA 
ATAGAAGAAC TCTGGAAAGA ACGTGAATTA GCACAAAGAG AATCCGAAGA ATTACAAGTT 
CAATTACATA TGACAGAAGA TAAAGTTGAT AGTTTACAAA ATCAATTGCA TGAAACTATT 
AGAAAACTTA AAGATGCTGA AAATCTTAAC GAAACATTAC GCAAAGAATT AGTAGATATT 
AGAAGACAAT TAGGTGATTC CACATATGAA AAAGAAAAGT ATAATAGTAG TAATAAAGAA 
TTACGCGAGC ATGTAAAACG CATTGAAAGC GAAAAAAGAG AACAAAATAG AATATTAGAA 
GAATCATATC AAAAAATTTC AGCACTGGAA GATATGAAAG TAAATGTAGA TGCAGAGAGA 
TCTCGTCTTC AAGCACAAAT TCGTGATATG GAGAAAGAAA TGTTGCAATT ACAAAAACAA 
CTTCATTTTA CTCAAGATGA ATTACAAAAA TCACATCAAA GTAATGCTCA AGCACAAAAT 
GATGAAAAAG AATTGCAAGC TCGACTAACT AACGAAACAG AAGAAAGAGA ACGTTTACAA 
TTGCAGTTAC ATCAAGTAAA AAAACAGTTA ATGGATGTTG ACAATAGTCT AAAAGTAACA 
AGACAAGAAC TTGGAAGATT GCGGTCACGT GCAGATGAAG AGAATGAACG GTGGAGAGTT 
AGAGAACAAG AACTAGTAGT TCGTCTTGAG GATAGTCGAT GTCGTGAAAG AAAACTCGAG 
GATCAAAAAC ATAACTTAGA AGTTTGTTTA GCTGATGCTT CTCAACAAAT TCAAGAACTG 
AAGGCACGTC TTGGAGGTTC CGAAGGACGA GTTCGAGCTT TAGATGCGCA ATTATCGCAA 
TTAGAAATAA CAAAAAAAGA AGTAGAACAA AAATTAAGTA GCGTGGGATC GACTTTACGT 
CGAATTGCAG GTATTCAAAT GGATGGAAGT GTTAATATAC CCTTTAAATT ATTGAGTCCA 
TCAAGAAGGT GGAGTCCAGC AAGAGCTCAA GATCATATTG ATTCTAGTAG AGATGTGATT 
CTTGATGTTG ATCCTGAGAT TATTAGAAAA GGTGTACGAT CTCTTATGCA ACAAGTAGCA 
CAAATCGAAC GTGAAAGAGA TGATTATAAA ACTGAATTAT GTAATTTGAA AAAACAATTG 
GATGAAAATC AAGAGATTCA AAATAGATCT GATATACAAA TAAACACTTT ATTAACAAAT 
ATAAGAATGC TTCAGGATGA AAAGAATTCA TTGGAGGTAA AACTTTCTCA AAAACAAAGT 
GGTTATGAAA TGCAGTCAGA AGAATGCGAG CAATTACGCG AGAAGATTAT CAATCTTGAA 
TTAATGATTA GTAATAATTC TGAAGAAAAA ATACAATTTG AGGAAAAACT AGATAAATTA 
AAACAAGTTT TAAACAAAGT AGAAAATGAA AAACGTAATT TACAAGAAGA ACTTAGTAAA 
AGCGAATCCC GCGCTACGAA ATTAGAATTA CAAAGAATGT CATTAGAAGG TGACCTTCAA 
AGATTGCAAA TGATGTTCCA AGAAAAAGAT GCAAATATTC ATAAATTACA AGAACGAAAT 
GATACACAAA ATCGAACAAT GACAACTTTA GAAGAACGTT GTACTTCATT GAAATCTATG 
GTGGAACAAT TAAATCTTGC ATTGGAAAAA GCATCTACTA CAGAAAATGA ATTAAAGAAT 
GAAATTAATT CAATGCAACA TAATATTATG GAATTAACAA CTACTTTGCA AACTTCTAAT 
GAAAAAAATA AACAGTTACA AAAACAAATT TCAAATGCTG AAAATGAACG TAGAATCTTA 
TCTGAACGTA TAGAATCAAT GCAACAATCT TTAAATGATC TCAAACATAC AAATCAAACA 
TTAACAGATC AAATTACACG TCTTCAAAAT GAATTGGCAA ATAATGAAGT ACAACGTTGT 
GCCTTAGAAT CTCAATTAAG AATAGTTGCC TATCCAACAC AAGAAGAAAA TATAAATAAG 
GATGAAGAAT TATTACGACA GTTACAAATA GCACAAAGAG AGAGAAGTGA AATGAGAGGA 
AAAATGGAAG CACTTAATGA TAAAATGAAA TTATTGGAAG CTGATAAACG TAATTTAGAA 
CGACAACTAT CGTTATTTAA ATCAACTAAT CGTAGCAAAA GTTATGAACG TTATGAGAAA 
GCACATACAG AATTATTAGG CACAAGTTTC GATATAGATC ATTATGAACA AGAAAATAGA 
GAATTAAGAT TGAAAGTTCG TAGATTAGAA ACACAACTTG CAGAGAAGGA AGCTGAACTT 
ATAAGATTGA AATCAAGTTA CACTCATACA CATTCCGTAT TTGATTTTAA TCGAGATAGA 
ACGGGCGAAA TTGAAAGACT TAGAGCAGCT CAATTACAAG CTGAAAAATT GTTAGAAGCA 
AGAGAACAAA GTCATCGTCA ACAAGTTTTG CGTTTGGAAA ATCAGATACA ATTGTTACGC 
GAGCAACTTA ATCAAGAAAT AAAACGTCGA CAATTATATG TTTTACGAAG TTCAAGGGCA 
GGTAGAGAAA TGCAACAATT AAGACAAGCC TTAGGCGATT CTTTAAGAAC TGTAGCTCAA 
GATCCATCAT TAGACGCAGT ATTATTAGAA CATGAAGCTC GAAAATTGGA TTCTACTTTG 
ACAAGTACTA CCAGTTTACC ACCATCATTA GCTTTACCTC CGCCACCGTC TTATGATAGA 
TCTTCCACAC CAGCACAACT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552019 ( XM_006559389.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006559452.1 Apis mellifera rootletin (LOC552019), transcript variant X2, mRNA.

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CloneID	OHd16376
Clone ID Related Accession (Same CDS sequence)	XM_006559389.2 , XM_006559389.3
Accession Version	XM_006559389.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	rootletin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007085.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006559389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC CATTGCCTAA AAATATTTCT CAATTATCAG CAAGAGGACA GATGGAGAGG 
CGGCGACCAC CGTTTCTCAG AGGCCCTAGT AGAATGAGAG AGAAAATAGA AAGAGGCATA 
GGCAGTAAAA TGGATTCTAC AGGAAGTACA CTAAGTGGAA CTGGAGATTG TTTAAGTGAA 
GAAGATCTTT CACCAGATGC ATTGGTTAGA CAAAACTATG AATTACGTCA TCGTCTTGAA 
GAAGAAGCAG CCAATTATAA AAGACGTTTG GATACATATA GACAAGCACA ACAACATCAG 
GCTGCTCTTG TTTCACGTTT ACAAGCCAAA GTATTGCAAT ATAAACAAAA ATGTTCAGAG 
TTAGAAAATC AAATGGCAGA ATCTATAGTA TATGATTCTA ATAAAGTTGT AAGTTCTGCA 
CCTCCAACAA CTTCTGTTTT AGATGCAGCT CATCAAACAC TAAGAGAAAT ACGTGAAGAA 
CAAATATATG ATTTAGATAC TGCTCTAAAA AAACTAAGTG AAGAACGTAA AAGATGTGAA 
AAATTGCTGC AATTAAATAC AACATTGAAA GATCAGTTAG AAGAATCTCA TCAAACAAAT 
GAAGCACTTA CAAATGATCT ACAAAAATTA AGTAATGATT GGGATATTTT AAGAGAAGAA 
TTAGCTATCA AAGAAGAAGA GTGGAGAGAA GAAGAGCAAG CTTTTAATGA TTATTATACT 
TCAGAACATA ACAGATTATT AATTCTTTGG CGCGATGTTG TTTCTGTAAA ACGATTATTT 
GCAGATATAA AATCTAGTAC AGAAAGGGAT CTATTAAAAA TGAAAAATAA CATTATTTCT 
ACTTTTAATG ATGTTTCATC AGCTTGTAAT AATACAGGAT TTGCTATGAG AATGCAAGCA 
GCTATGCATC CAATGATATC TCAACAAGTT CAACAGGCAC AAGAACAGGT AACAACTGAT 
TTAAAAACAG AATTAATAAC ACTTAAACAA CAGTATGATG TAGCTCAAAA TGAAATTCGT 
ATAAAGGAAG AAAAAATCAA TCAACTTATT CGTGATGTTC ACAGTTTGGA AGAAAGATGT 
GGAGAAGCAG AAACAGAAAT TCATCGCAAT ACACGTTTGC AAGATGATAT AGAAATTTTA 
CAATCTGCAT TGAGAGATAT AGCACATGCT GTAATTCAAG ATGCAGAAAG TCGCGAAATT 
GAATCAACTC AAGCACCTCC TCATCTTCAT TTATCACCTA CTGGACCAAT TCCTCAAAAA 
TCACCAAAAA GAGGTACAAG AAGCAACACC ATTCCAGCTT TTGCTGAAAG TACTATAAGT 
GCTGTACAAG CAGCTCTCCA TAAATATCAA TTGACTATAC ATGAACTTCA GGTGAAATTG 
CAAACTAATA AAGAACAACT ACAAAATACT CGCAAACAAT GCGAAAATGC GGAAGAGAAT 
GTTAAAACTC TAGAGAAAAA AGCAGAAGAA CTGATTATTG AATTAGATGC AGTTCGATTG 
CACTGTTCAC AACTTAATCA AGAAAAAGAT ATGTTACAAA AAGGACTTGA TACTATAAGA 
ATAGAAAAAA ATACTCTTGA AAAAACCAGG ATAGAACTTA ATAATATGAT GGAGAATTTA 
AACAATGATT GCGAAAAATT GCAAAAAGCT AACAACAAAT TGCAAAAAAT TTGCGACAAT 
TTAGAAGATG AAAAATTATA TCTTCAAAGT GAACTCAATA GATTATCTAA AGATGTAGAA 
TTGAGGGAAT TAAATTTGCG TTCAGAAGAA GATAGGTGCA GTAAAATGAG AGAGGAATTA 
TTAACTCTAC GTGAAGAATT GAATAAAACA TATCTCAGTA AAGATATGTT AGAACAACAA 
AAGTTAGAAA CAGATGGATT AATTTCACAA ATTGAAAAAA GCAAAGGTGA TTTAGAATTA 
GAATTGGAAC GCGTATTATT AGAAAAATCA GACGTGCAAG AAGTTTTAAT GAAACTAGAA 
ACAGTTTGCA GCAATCATGA ACAAGAGAAA CAACGATTAC AAGAAGAATT AAAAAAGGTA 
ACAGAAGAAA AAAATAAGTT AGCAAATCAA TGTACGGATC AACAAGGTGA TCTGGGTTCC 
TTAAGAAAAG AATTACTTCA AGCAGAACAA ACTAGACTTG ATTTAGAATC AGAAAAAGTA 
ACTTTGAATG AAAAAGTGAA ATTTCTGGAG ATCGAAAAGG AGAAGATTGA AATAGAATTG 
GCACAAGTTA CGCGCGAACG TGGAGATTTC AATAATCAAT TATCAGTTTT AGCACGAAAG 
AAAGAAACAT TAAACGAAGA ATTGATGAGA CTCAAACAAA GATTAGAACA GGCAAACGAA 
ATGAATGGAA GAATAAATCG GAATTTAGAA GATCTTGTAA AAGATAATGA AGAAAAACAA 
GTTCTTTTAG AAACTAATGA AAAGGAAGTA CAACGATTAC AAGAGCAACT TGCTTCAATG 
CGTAGTGAGA AAGAGACATT AGAAGGTGTT TTATTTGATA CACAAACTAG TCTAGAAAAT 
ATGCATGTGA AAAAGACTCA ATTAGAAAAG GAACAGAAAG AACTTTTAAT AAAACAAGAA 
AGCTTAAAAG GACAAGTAGA AAGATTAATG AAAGATTTGG AGAATAGCGA AAAACGAATT 
CATGAAGTAA AGCAAACTTT AACGCAACAA AGTGGCGATC AAGAAGCCGA ATTTCAACAA 
ATTATTTCTA ACGTGAAAAA ACATAGTGAA GAAAACATAA AAAAATTAAA TGAAGAAAAA 
GAACAAATAA AGATAAATTT AGAAAAACGA CTTCAGCAAT CTCTCTTGCA AGTTACTGGA 
GAAAAAGATA ATGAAATAAA TCAATTGCAG CAAAGAATAG ATGAAATGCA ACAACATATA 
GAAAATTTGT GTCAGCAACA TGAAGAAGTT CTTCTTAGAG CAGAAAATGA TAAACAGCAA 
GCTCTTCTTA TAGCTCATCA TGATCAGCAA GCATTAATGG AAAAACTTGA AACTGTTTTG 
CATGAAATGG AAGAAGAGAA AAATAATGTA GAACGAGTCA AACGAGAAGC TGCAGTAAAA 
ACTGAACAAG AGCGTAATAA TATAAATCAA TTACGTGATG AATTAAATCG TCTTAAAACA 
AAATTAGATG AAACTAGATT AAAAACTGAT GAAGAAAAAA TAAAACTTGA TCTAAAAATA 
GAAGAACTCT GGAAAGAACG TGAATTAGCA CAAAGAGAAT CCGAAGAATT ACAAGTTCAA 
TTACATATGA CAGAAGATAA AGTTGATAGT TTACAAAATC AATTGCATGA AACTATTAGA 
AAACTTAAAG ATGCTGAAAA TCTTAACGAA ACATTACGCA AAGAATTAGT AGATATTAGA 
AGACAATTAG GTGATTCCAC ATATGAAAAA GAAAAGTATA ATAGTAGTAA TAAAGAATTA 
CGCGAGCATG TAAAACGCAT TGAAAGCGAA AAAAGAGAAC AAAATAGAAT ATTAGAAGAA 
TCATATCAAA AAATTTCAGC ACTGGAAGAT ATGAAAGTAA ATGTAGATGC AGAGAGATCT 
CGTCTTCAAG CACAAATTCG TGATATGGAG AAAGAAATGT TGCAATTACA AAAACAACTT 
CATTTTACTC AAGATGAATT ACAAAAATCA CATCAAAGTA ATGCTCAAGC ACAAAATGAT 
GAAAAAGAAT TGCAAGCTCG ACTAACTAAC GAAACAGAAG AAAGAGAACG TTTACAATTG 
CAGTTACATC AAGTAAAAAA ACAGTTAATG GATGTTGACA ATAGTCTAAA AGTAACAAGA 
CAAGAACTTG GAAGATTGCG GTCACGTGCA GATGAAGAGA ATGAACGGTG GAGAGTTAGA 
GAACAAGAAC TAGTAGTTCG TCTTGAGGAT AGTCGATGTC GTGAAAGAAA ACTCGAGGAT 
CAAAAACATA ACTTAGAAGT TTGTTTAGCT GATGCTTCTC AACAAATTCA AGAACTGAAG 
GCACGTCTTG GAGGTTCCGA AGGACGAGTT CGAGCTTTAG ATGCGCAATT ATCGCAATTA 
GAAATAACAA AAAAAGAAGT AGAACAAAAA TTAAGTAGCG TGGGATCGAC TTTACGTCGA 
ATTGCAGGTA TTCAAATGGA TGGAAGTGTT AATATACCCT TTAAATTATT GAGTCCATCA 
AGAAGGTGGA GTCCAGCAAG AGCTCAAGAT CATATTGATT CTAGTAGAGA TGTGATTCTT 
GATGTTGATC CTGAGATTAT TAGAAAAGGT GTACGATCTC TTATGCAACA AGTAGCACAA 
ATCGAACGTG AAAGAGATGA TTATAAAACT GAATTATGTA ATTTGAAAAA ACAATTGGAT 
GAAAATCAAG AGATTCAAAA TAGATCTGAT ATACAAATAA ACACTTTATT AACAAATATA 
AGAATGCTTC AGGATGAAAA GAATTCATTG GAGGTAAAAC TTTCTCAAAA ACAAAGTGGT 
TATGAAATGC AGTTAAATGC TTTACAACTA AAGTCAGAAG AATGCGAGCA ATTACGCGAG 
AAGATTATCA ATCTTGAATT AATGATTAGT AATAATTCTG AAGAAAAAAT ACAATTTGAG 
GAAAAACTAG ATAAATTAAA ACAAGTTTTA AACAAAGTAG AAAATGAAAA ACGTAATTTA 
CAAGAAGAAC TTAGTAAAAG CGAATCCCGC GCTACGAAAT TAGAATTACA AAGAATGTCA 
TTAGAAGGTG ACCTTCAAAG ATTGCAAATG ATGTTCCAAG AAAAAGATGC AAATATTCAT 
AAATTACAAG AACGAAATGA TACACAAAAT CGAACAATGA CAACTTTAGA AGAACGTTGT 
ACTTCATTGA AATCTATGGT GGAACAATTA AATCTTGCAT TGGAAAAAGC ATCTACTACA 
GAAAATGAAT TAAAGAATGA AATTAATTCA ATGCAACATA ATATTATGGA ATTAACAACT 
ACTTTGCAAA CTTCTAATGA AAAAAATAAA CAGTTACAAA AACAAATTTC AAATGCTGAA 
AATGAACGTA GAATCTTATC TGAACGTATA GAATCAATGC AACAATCTTT AAATGATCTC 
AAACATACAA ATCAAACATT AACAGATCAA ATTACACGTC TTCAAAATGA ATTGGCAAAT 
AATGAAGTAC AACGTTGTGC CTTAGAATCT CAATTAAGAA TAGTTGCCTA TCCAACACAA 
GAAGAAAATA TAAATAAGGA TGAAGAATTA TTACGACAGT TACAAATAGC ACAAAGAGAG 
AGAAGTGAAA TGAGAGGAAA AATGGAAGCA CTTAATGATA AAATGAAATT ATTGGAAGCT 
GATAAACGTA ATTTAGAACG ACAACTATCG TTATTTAAAT CAACTAATCG TAGCAAAAGT 
TATGAACGTT ATGAGAAAGC ACATACAGAA TTATTAGGCA CAAGTTTCGA TATAGATCAT 
TATGAACAAG AAAATAGAGA ATTAAGATTG AAAGTTCGTA GATTAGAAAC ACAACTTGCA 
GAGAAGGAAG CTGAACTTAT AAGATTGAAA TCAAGTTACA CTCATACACA TTCCGTATTT 
GATTTTAATC GAGATAGAAC GGGCGAAATT GAAAGACTTA GAGCAGCTCA ATTACAAGCT 
GAAAAATTGT TAGAAGCAAG AGAACAAAGT CATCGTCAAC AAGTTTTGCG TTTGGAAAAT 
CAGATACAAT TGTTACGCGA GCAACTTAAT CAAGAAATAA AACGTCGACA ATTATATGTT 
TTACGAAGTT CAAGGGCAGG TAGAGAAATG CAACAATTAA GACAAGCCTT AGGCGATTCT 
TTAAGAACTG TAGCTCAAGA TCCATCATTA GACGCAGTAT TATTAGAACA TGAAGCTCGA 
AAATTGGATT CTACTTTGAC AAGTACTACC AGTTTACCAC CATCATTAGC TTTACCTCCG 
CCACCGTCTT ATGATAGATC TTCCACACCA GCACAACTCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006559452.1
CDS	146..6070
Translation	MSRPLPKNISQLSARGQMERRRPPFLRGPSRMREKIERGIGSKMDSTGSTLSGTGDCLSEEDLSPDALVRQNYELRHRLEEEAANYKRRLDTYRQAQQHQAALVSRLQAKVLQYKQKCSELENQMAESIVYDSNKVVSSAPPTTSVLDAAHQTLREIREEQIYDLDTALKKLSEERKRCEKLLQLNTTLKDQLEESHQTNEALTNDLQKLSNDWDILREELAIKEEEWREEEQAFNDYYTSEHNRLLILWRDVVSVKRLFADIKSSTERDLLKMKNNIISTFNDVSSACNNTGFAMRMQAAMHPMISQQVQQAQEQVTTDLKTELITLKQQYDVAQNEIRIKEEKINQLIRDVHSLEERCGEAETEIHRNTRLQDDIEILQSALRDIAHAVIQDAESREIESTQAPPHLHLSPTGPIPQKSPKRGTRSNTIPAFAESTISAVQAALHKYQLTIHELQVKLQTNKEQLQNTRKQCENAEENVKTLEKKAEELIIELDAVRLHCSQLNQEKDMLQKGLDTIRIEKNTLEKTRIELNNMMENLNNDCEKLQKANNKLQKICDNLEDEKLYLQSELNRLSKDVELRELNLRSEEDRCSKMREELLTLREELNKTYLSKDMLEQQKLETDGLISQIEKSKGDLELELERVLLEKSDVQEVLMKLETVCSNHEQEKQRLQEELKKVTEEKNKLANQCTDQQGDLGSLRKELLQAEQTRLDLESEKVTLNEKVKFLEIEKEKIEIELAQVTRERGDFNNQLSVLARKKETLNEELMRLKQRLEQANEMNGRINRNLEDLVKDNEEKQVLLETNEKEVQRLQEQLASMRSEKETLEGVLFDTQTSLENMHVKKTQLEKEQKELLIKQESLKGQVERLMKDLENSEKRIHEVKQTLTQQSGDQEAEFQQIISNVKKHSEENIKKLNEEKEQIKINLEKRLQQSLLQVTGEKDNEINQLQQRIDEMQQHIENLCQQHEEVLLRAENDKQQALLIAHHDQQALMEKLETVLHEMEEEKNNVERVKREAAVKTEQERNNINQLRDELNRLKTKLDETRLKTDEEKIKLDLKIEELWKERELAQRESEELQVQLHMTEDKVDSLQNQLHETIRKLKDAENLNETLRKELVDIRRQLGDSTYEKEKYNSSNKELREHVKRIESEKREQNRILEESYQKISALEDMKVNVDAERSRLQAQIRDMEKEMLQLQKQLHFTQDELQKSHQSNAQAQNDEKELQARLTNETEERERLQLQLHQVKKQLMDVDNSLKVTRQELGRLRSRADEENERWRVREQELVVRLEDSRCRERKLEDQKHNLEVCLADASQQIQELKARLGGSEGRVRALDAQLSQLEITKKEVEQKLSSVGSTLRRIAGIQMDGSVNIPFKLLSPSRRWSPARAQDHIDSSRDVILDVDPEIIRKGVRSLMQQVAQIERERDDYKTELCNLKKQLDENQEIQNRSDIQINTLLTNIRMLQDEKNSLEVKLSQKQSGYEMQLNALQLKSEECEQLREKIINLELMISNNSEEKIQFEEKLDKLKQVLNKVENEKRNLQEELSKSESRATKLELQRMSLEGDLQRLQMMFQEKDANIHKLQERNDTQNRTMTTLEERCTSLKSMVEQLNLALEKASTTENELKNEINSMQHNIMELTTTLQTSNEKNKQLQKQISNAENERRILSERIESMQQSLNDLKHTNQTLTDQITRLQNELANNEVQRCALESQLRIVAYPTQEENINKDEELLRQLQIAQRERSEMRGKMEALNDKMKLLEADKRNLERQLSLFKSTNRSKSYERYEKAHTELLGTSFDIDHYEQENRELRLKVRRLETQLAEKEAELIRLKSSYTHTHSVFDFNRDRTGEIERLRAAQLQAEKLLEAREQSHRQQVLRLENQIQLLREQLNQEIKRRQLYVLRSSRAGREMQQLRQALGDSLRTVAQDPSLDAVLLEHEARKLDSTLTSTTSLPPSLALPPPPSYDRSSTPAQLK

Target ORF information:

RefSeq Version	XM_006559389.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera rootletin (LOC552019), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006559389.2

ORF Insert Sequence:

ATGTCACGGC CATTGCCTAA AAATATTTCT CAATTATCAG CAAGAGGACA GATGGAGAGG 
CGGCGACCAC CGTTTCTCAG AGGCCCTAGT AGAATGAGAG AGAAAATAGA AAGAGGCATA 
GGCAGTAAAA TGGATTCTAC AGGAAGTACA CTAAGTGGAA CTGGAGATTG TTTAAGTGAA 
GAAGATCTTT CACCAGATGC ATTGGTTAGA CAAAACTATG AATTACGTCA TCGTCTTGAA 
GAAGAAGCAG CCAATTATAA AAGACGTTTG GATACATATA GACAAGCACA ACAACATCAG 
GCTGCTCTTG TTTCACGTTT ACAAGCCAAA GTATTGCAAT ATAAACAAAA ATGTTCAGAG 
TTAGAAAATC AAATGGCAGA ATCTATAGTA TATGATTCTA ATAAAGTTGT AAGTTCTGCA 
CCTCCAACAA CTTCTGTTTT AGATGCAGCT CATCAAACAC TAAGAGAAAT ACGTGAAGAA 
CAAATATATG ATTTAGATAC TGCTCTAAAA AAACTAAGTG AAGAACGTAA AAGATGTGAA 
AAATTGCTGC AATTAAATAC AACATTGAAA GATCAGTTAG AAGAATCTCA TCAAACAAAT 
GAAGCACTTA CAAATGATCT ACAAAAATTA AGTAATGATT GGGATATTTT AAGAGAAGAA 
TTAGCTATCA AAGAAGAAGA GTGGAGAGAA GAAGAGCAAG CTTTTAATGA TTATTATACT 
TCAGAACATA ACAGATTATT AATTCTTTGG CGCGATGTTG TTTCTGTAAA ACGATTATTT 
GCAGATATAA AATCTAGTAC AGAAAGGGAT CTATTAAAAA TGAAAAATAA CATTATTTCT 
ACTTTTAATG ATGTTTCATC AGCTTGTAAT AATACAGGAT TTGCTATGAG AATGCAAGCA 
GCTATGCATC CAATGATATC TCAACAAGTT CAACAGGCAC AAGAACAGGT AACAACTGAT 
TTAAAAACAG AATTAATAAC ACTTAAACAA CAGTATGATG TAGCTCAAAA TGAAATTCGT 
ATAAAGGAAG AAAAAATCAA TCAACTTATT CGTGATGTTC ACAGTTTGGA AGAAAGATGT 
GGAGAAGCAG AAACAGAAAT TCATCGCAAT ACACGTTTGC AAGATGATAT AGAAATTTTA 
CAATCTGCAT TGAGAGATAT AGCACATGCT GTAATTCAAG ATGCAGAAAG TCGCGAAATT 
GAATCAACTC AAGCACCTCC TCATCTTCAT TTATCACCTA CTGGACCAAT TCCTCAAAAA 
TCACCAAAAA GAGGTACAAG AAGCAACACC ATTCCAGCTT TTGCTGAAAG TACTATAAGT 
GCTGTACAAG CAGCTCTCCA TAAATATCAA TTGACTATAC ATGAACTTCA GGTGAAATTG 
CAAACTAATA AAGAACAACT ACAAAATACT CGCAAACAAT GCGAAAATGC GGAAGAGAAT 
GTTAAAACTC TAGAGAAAAA AGCAGAAGAA CTGATTATTG AATTAGATGC AGTTCGATTG 
CACTGTTCAC AACTTAATCA AGAAAAAGAT ATGTTACAAA AAGGACTTGA TACTATAAGA 
ATAGAAAAAA ATACTCTTGA AAAAACCAGG ATAGAACTTA ATAATATGAT GGAGAATTTA 
AACAATGATT GCGAAAAATT GCAAAAAGCT AACAACAAAT TGCAAAAAAT TTGCGACAAT 
TTAGAAGATG AAAAATTATA TCTTCAAAGT GAACTCAATA GATTATCTAA AGATGTAGAA 
TTGAGGGAAT TAAATTTGCG TTCAGAAGAA GATAGGTGCA GTAAAATGAG AGAGGAATTA 
TTAACTCTAC GTGAAGAATT GAATAAAACA TATCTCAGTA AAGATATGTT AGAACAACAA 
AAGTTAGAAA CAGATGGATT AATTTCACAA ATTGAAAAAA GCAAAGGTGA TTTAGAATTA 
GAATTGGAAC GCGTATTATT AGAAAAATCA GACGTGCAAG AAGTTTTAAT GAAACTAGAA 
ACAGTTTGCA GCAATCATGA ACAAGAGAAA CAACGATTAC AAGAAGAATT AAAAAAGGTA 
ACAGAAGAAA AAAATAAGTT AGCAAATCAA TGTACGGATC AACAAGGTGA TCTGGGTTCC 
TTAAGAAAAG AATTACTTCA AGCAGAACAA ACTAGACTTG ATTTAGAATC AGAAAAAGTA 
ACTTTGAATG AAAAAGTGAA ATTTCTGGAG ATCGAAAAGG AGAAGATTGA AATAGAATTG 
GCACAAGTTA CGCGCGAACG TGGAGATTTC AATAATCAAT TATCAGTTTT AGCACGAAAG 
AAAGAAACAT TAAACGAAGA ATTGATGAGA CTCAAACAAA GATTAGAACA GGCAAACGAA 
ATGAATGGAA GAATAAATCG GAATTTAGAA GATCTTGTAA AAGATAATGA AGAAAAACAA 
GTTCTTTTAG AAACTAATGA AAAGGAAGTA CAACGATTAC AAGAGCAACT TGCTTCAATG 
CGTAGTGAGA AAGAGACATT AGAAGGTGTT TTATTTGATA CACAAACTAG TCTAGAAAAT 
ATGCATGTGA AAAAGACTCA ATTAGAAAAG GAACAGAAAG AACTTTTAAT AAAACAAGAA 
AGCTTAAAAG GACAAGTAGA AAGATTAATG AAAGATTTGG AGAATAGCGA AAAACGAATT 
CATGAAGTAA AGCAAACTTT AACGCAACAA AGTGGCGATC AAGAAGCCGA ATTTCAACAA 
ATTATTTCTA ACGTGAAAAA ACATAGTGAA GAAAACATAA AAAAATTAAA TGAAGAAAAA 
GAACAAATAA AGATAAATTT AGAAAAACGA CTTCAGCAAT CTCTCTTGCA AGTTACTGGA 
GAAAAAGATA ATGAAATAAA TCAATTGCAG CAAAGAATAG ATGAAATGCA ACAACATATA 
GAAAATTTGT GTCAGCAACA TGAAGAAGTT CTTCTTAGAG CAGAAAATGA TAAACAGCAA 
GCTCTTCTTA TAGCTCATCA TGATCAGCAA GCATTAATGG AAAAACTTGA AACTGTTTTG 
CATGAAATGG AAGAAGAGAA AAATAATGTA GAACGAGTCA AACGAGAAGC TGCAGTAAAA 
ACTGAACAAG AGCGTAATAA TATAAATCAA TTACGTGATG AATTAAATCG TCTTAAAACA 
AAATTAGATG AAACTAGATT AAAAACTGAT GAAGAAAAAA TAAAACTTGA TCTAAAAATA 
GAAGAACTCT GGAAAGAACG TGAATTAGCA CAAAGAGAAT CCGAAGAATT ACAAGTTCAA 
TTACATATGA CAGAAGATAA AGTTGATAGT TTACAAAATC AATTGCATGA AACTATTAGA 
AAACTTAAAG ATGCTGAAAA TCTTAACGAA ACATTACGCA AAGAATTAGT AGATATTAGA 
AGACAATTAG GTGATTCCAC ATATGAAAAA GAAAAGTATA ATAGTAGTAA TAAAGAATTA 
CGCGAGCATG TAAAACGCAT TGAAAGCGAA AAAAGAGAAC AAAATAGAAT ATTAGAAGAA 
TCATATCAAA AAATTTCAGC ACTGGAAGAT ATGAAAGTAA ATGTAGATGC AGAGAGATCT 
CGTCTTCAAG CACAAATTCG TGATATGGAG AAAGAAATGT TGCAATTACA AAAACAACTT 
CATTTTACTC AAGATGAATT ACAAAAATCA CATCAAAGTA ATGCTCAAGC ACAAAATGAT 
GAAAAAGAAT TGCAAGCTCG ACTAACTAAC GAAACAGAAG AAAGAGAACG TTTACAATTG 
CAGTTACATC AAGTAAAAAA ACAGTTAATG GATGTTGACA ATAGTCTAAA AGTAACAAGA 
CAAGAACTTG GAAGATTGCG GTCACGTGCA GATGAAGAGA ATGAACGGTG GAGAGTTAGA 
GAACAAGAAC TAGTAGTTCG TCTTGAGGAT AGTCGATGTC GTGAAAGAAA ACTCGAGGAT 
CAAAAACATA ACTTAGAAGT TTGTTTAGCT GATGCTTCTC AACAAATTCA AGAACTGAAG 
GCACGTCTTG GAGGTTCCGA AGGACGAGTT CGAGCTTTAG ATGCGCAATT ATCGCAATTA 
GAAATAACAA AAAAAGAAGT AGAACAAAAA TTAAGTAGCG TGGGATCGAC TTTACGTCGA 
ATTGCAGGTA TTCAAATGGA TGGAAGTGTT AATATACCCT TTAAATTATT GAGTCCATCA 
AGAAGGTGGA GTCCAGCAAG AGCTCAAGAT CATATTGATT CTAGTAGAGA TGTGATTCTT 
GATGTTGATC CTGAGATTAT TAGAAAAGGT GTACGATCTC TTATGCAACA AGTAGCACAA 
ATCGAACGTG AAAGAGATGA TTATAAAACT GAATTATGTA ATTTGAAAAA ACAATTGGAT 
GAAAATCAAG AGATTCAAAA TAGATCTGAT ATACAAATAA ACACTTTATT AACAAATATA 
AGAATGCTTC AGGATGAAAA GAATTCATTG GAGGTAAAAC TTTCTCAAAA ACAAAGTGGT 
TATGAAATGC AGTTAAATGC TTTACAACTA AAGTCAGAAG AATGCGAGCA ATTACGCGAG 
AAGATTATCA ATCTTGAATT AATGATTAGT AATAATTCTG AAGAAAAAAT ACAATTTGAG 
GAAAAACTAG ATAAATTAAA ACAAGTTTTA AACAAAGTAG AAAATGAAAA ACGTAATTTA 
CAAGAAGAAC TTAGTAAAAG CGAATCCCGC GCTACGAAAT TAGAATTACA AAGAATGTCA 
TTAGAAGGTG ACCTTCAAAG ATTGCAAATG ATGTTCCAAG AAAAAGATGC AAATATTCAT 
AAATTACAAG AACGAAATGA TACACAAAAT CGAACAATGA CAACTTTAGA AGAACGTTGT 
ACTTCATTGA AATCTATGGT GGAACAATTA AATCTTGCAT TGGAAAAAGC ATCTACTACA 
GAAAATGAAT TAAAGAATGA AATTAATTCA ATGCAACATA ATATTATGGA ATTAACAACT 
ACTTTGCAAA CTTCTAATGA AAAAAATAAA CAGTTACAAA AACAAATTTC AAATGCTGAA 
AATGAACGTA GAATCTTATC TGAACGTATA GAATCAATGC AACAATCTTT AAATGATCTC 
AAACATACAA ATCAAACATT AACAGATCAA ATTACACGTC TTCAAAATGA ATTGGCAAAT 
AATGAAGTAC AACGTTGTGC CTTAGAATCT CAATTAAGAA TAGTTGCCTA TCCAACACAA 
GAAGAAAATA TAAATAAGGA TGAAGAATTA TTACGACAGT TACAAATAGC ACAAAGAGAG 
AGAAGTGAAA TGAGAGGAAA AATGGAAGCA CTTAATGATA AAATGAAATT ATTGGAAGCT 
GATAAACGTA ATTTAGAACG ACAACTATCG TTATTTAAAT CAACTAATCG TAGCAAAAGT 
TATGAACGTT ATGAGAAAGC ACATACAGAA TTATTAGGCA CAAGTTTCGA TATAGATCAT 
TATGAACAAG AAAATAGAGA ATTAAGATTG AAAGTTCGTA GATTAGAAAC ACAACTTGCA 
GAGAAGGAAG CTGAACTTAT AAGATTGAAA TCAAGTTACA CTCATACACA TTCCGTATTT 
GATTTTAATC GAGATAGAAC GGGCGAAATT GAAAGACTTA GAGCAGCTCA ATTACAAGCT 
GAAAAATTGT TAGAAGCAAG AGAACAAAGT CATCGTCAAC AAGTTTTGCG TTTGGAAAAT 
CAGATACAAT TGTTACGCGA GCAACTTAAT CAAGAAATAA AACGTCGACA ATTATATGTT 
TTACGAAGTT CAAGGGCAGG TAGAGAAATG CAACAATTAA GACAAGCCTT AGGCGATTCT 
TTAAGAACTG TAGCTCAAGA TCCATCATTA GACGCAGTAT TATTAGAACA TGAAGCTCGA 
AAATTGGATT CTACTTTGAC AAGTACTACC AGTTTACCAC CATCATTAGC TTTACCTCCG 
CCACCGTCTT ATGATAGATC TTCCACACCA GCACAACTCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552019 ( XM_006559389.3 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006559452.1 Apis mellifera rootletin (LOC552019), transcript variant X1, mRNA.

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CloneID	OHd16376
Clone ID Related Accession (Same CDS sequence)	XM_006559389.2 , XM_006559389.3
Accession Version	XM_006559389.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	rootletin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_006559389.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC CATTGCCTAA AAATATTTCT CAATTATCAG CAAGAGGACA GATGGAGAGG 
CGGCGACCAC CGTTTCTCAG AGGCCCTAGT AGAATGAGAG AGAAAATAGA AAGAGGCATA 
GGCAGTAAAA TGGATTCTAC AGGAAGTACA CTAAGTGGAA CTGGAGATTG TTTAAGTGAA 
GAAGATCTTT CACCAGATGC ATTGGTTAGA CAAAACTATG AATTACGTCA TCGTCTTGAA 
GAAGAAGCAG CCAATTATAA AAGACGTTTG GATACATATA GACAAGCACA ACAACATCAG 
GCTGCTCTTG TTTCACGTTT ACAAGCCAAA GTATTGCAAT ATAAACAAAA ATGTTCAGAG 
TTAGAAAATC AAATGGCAGA ATCTATAGTA TATGATTCTA ATAAAGTTGT AAGTTCTGCA 
CCTCCAACAA CTTCTGTTTT AGATGCAGCT CATCAAACAC TAAGAGAAAT ACGTGAAGAA 
CAAATATATG ATTTAGATAC TGCTCTAAAA AAACTAAGTG AAGAACGTAA AAGATGTGAA 
AAATTGCTGC AATTAAATAC AACATTGAAA GATCAGTTAG AAGAATCTCA TCAAACAAAT 
GAAGCACTTA CAAATGATCT ACAAAAATTA AGTAATGATT GGGATATTTT AAGAGAAGAA 
TTAGCTATCA AAGAAGAAGA GTGGAGAGAA GAAGAGCAAG CTTTTAATGA TTATTATACT 
TCAGAACATA ACAGATTATT AATTCTTTGG CGCGATGTTG TTTCTGTAAA ACGATTATTT 
GCAGATATAA AATCTAGTAC AGAAAGGGAT CTATTAAAAA TGAAAAATAA CATTATTTCT 
ACTTTTAATG ATGTTTCATC AGCTTGTAAT AATACAGGAT TTGCTATGAG AATGCAAGCA 
GCTATGCATC CAATGATATC TCAACAAGTT CAACAGGCAC AAGAACAGGT AACAACTGAT 
TTAAAAACAG AATTAATAAC ACTTAAACAA CAGTATGATG TAGCTCAAAA TGAAATTCGT 
ATAAAGGAAG AAAAAATCAA TCAACTTATT CGTGATGTTC ACAGTTTGGA AGAAAGATGT 
GGAGAAGCAG AAACAGAAAT TCATCGCAAT ACACGTTTGC AAGATGATAT AGAAATTTTA 
CAATCTGCAT TGAGAGATAT AGCACATGCT GTAATTCAAG ATGCAGAAAG TCGCGAAATT 
GAATCAACTC AAGCACCTCC TCATCTTCAT TTATCACCTA CTGGACCAAT TCCTCAAAAA 
TCACCAAAAA GAGGTACAAG AAGCAACACC ATTCCAGCTT TTGCTGAAAG TACTATAAGT 
GCTGTACAAG CAGCTCTCCA TAAATATCAA TTGACTATAC ATGAACTTCA GGTGAAATTG 
CAAACTAATA AAGAACAACT ACAAAATACT CGCAAACAAT GCGAAAATGC GGAAGAGAAT 
GTTAAAACTC TAGAGAAAAA AGCAGAAGAA CTGATTATTG AATTAGATGC AGTTCGATTG 
CACTGTTCAC AACTTAATCA AGAAAAAGAT ATGTTACAAA AAGGACTTGA TACTATAAGA 
ATAGAAAAAA ATACTCTTGA AAAAACCAGG ATAGAACTTA ATAATATGAT GGAGAATTTA 
AACAATGATT GCGAAAAATT GCAAAAAGCT AACAACAAAT TGCAAAAAAT TTGCGACAAT 
TTAGAAGATG AAAAATTATA TCTTCAAAGT GAACTCAATA GATTATCTAA AGATGTAGAA 
TTGAGGGAAT TAAATTTGCG TTCAGAAGAA GATAGGTGCA GTAAAATGAG AGAGGAATTA 
TTAACTCTAC GTGAAGAATT GAATAAAACA TATCTCAGTA AAGATATGTT AGAACAACAA 
AAGTTAGAAA CAGATGGATT AATTTCACAA ATTGAAAAAA GCAAAGGTGA TTTAGAATTA 
GAATTGGAAC GCGTATTATT AGAAAAATCA GACGTGCAAG AAGTTTTAAT GAAACTAGAA 
ACAGTTTGCA GCAATCATGA ACAAGAGAAA CAACGATTAC AAGAAGAATT AAAAAAGGTA 
ACAGAAGAAA AAAATAAGTT AGCAAATCAA TGTACGGATC AACAAGGTGA TCTGGGTTCC 
TTAAGAAAAG AATTACTTCA AGCAGAACAA ACTAGACTTG ATTTAGAATC AGAAAAAGTA 
ACTTTGAATG AAAAAGTGAA ATTTCTGGAG ATCGAAAAGG AGAAGATTGA AATAGAATTG 
GCACAAGTTA CGCGCGAACG TGGAGATTTC AATAATCAAT TATCAGTTTT AGCACGAAAG 
AAAGAAACAT TAAACGAAGA ATTGATGAGA CTCAAACAAA GATTAGAACA GGCAAACGAA 
ATGAATGGAA GAATAAATCG GAATTTAGAA GATCTTGTAA AAGATAATGA AGAAAAACAA 
GTTCTTTTAG AAACTAATGA AAAGGAAGTA CAACGATTAC AAGAGCAACT TGCTTCAATG 
CGTAGTGAGA AAGAGACATT AGAAGGTGTT TTATTTGATA CACAAACTAG TCTAGAAAAT 
ATGCATGTGA AAAAGACTCA ATTAGAAAAG GAACAGAAAG AACTTTTAAT AAAACAAGAA 
AGCTTAAAAG GACAAGTAGA AAGATTAATG AAAGATTTGG AGAATAGCGA AAAACGAATT 
CATGAAGTAA AGCAAACTTT AACGCAACAA AGTGGCGATC AAGAAGCCGA ATTTCAACAA 
ATTATTTCTA ACGTGAAAAA ACATAGTGAA GAAAACATAA AAAAATTAAA TGAAGAAAAA 
GAACAAATAA AGATAAATTT AGAAAAACGA CTTCAGCAAT CTCTCTTGCA AGTTACTGGA 
GAAAAAGATA ATGAAATAAA TCAATTGCAG CAAAGAATAG ATGAAATGCA ACAACATATA 
GAAAATTTGT GTCAGCAACA TGAAGAAGTT CTTCTTAGAG CAGAAAATGA TAAACAGCAA 
GCTCTTCTTA TAGCTCATCA TGATCAGCAA GCATTAATGG AAAAACTTGA AACTGTTTTG 
CATGAAATGG AAGAAGAGAA AAATAATGTA GAACGAGTCA AACGAGAAGC TGCAGTAAAA 
ACTGAACAAG AGCGTAATAA TATAAATCAA TTACGTGATG AATTAAATCG TCTTAAAACA 
AAATTAGATG AAACTAGATT AAAAACTGAT GAAGAAAAAA TAAAACTTGA TCTAAAAATA 
GAAGAACTCT GGAAAGAACG TGAATTAGCA CAAAGAGAAT CCGAAGAATT ACAAGTTCAA 
TTACATATGA CAGAAGATAA AGTTGATAGT TTACAAAATC AATTGCATGA AACTATTAGA 
AAACTTAAAG ATGCTGAAAA TCTTAACGAA ACATTACGCA AAGAATTAGT AGATATTAGA 
AGACAATTAG GTGATTCCAC ATATGAAAAA GAAAAGTATA ATAGTAGTAA TAAAGAATTA 
CGCGAGCATG TAAAACGCAT TGAAAGCGAA AAAAGAGAAC AAAATAGAAT ATTAGAAGAA 
TCATATCAAA AAATTTCAGC ACTGGAAGAT ATGAAAGTAA ATGTAGATGC AGAGAGATCT 
CGTCTTCAAG CACAAATTCG TGATATGGAG AAAGAAATGT TGCAATTACA AAAACAACTT 
CATTTTACTC AAGATGAATT ACAAAAATCA CATCAAAGTA ATGCTCAAGC ACAAAATGAT 
GAAAAAGAAT TGCAAGCTCG ACTAACTAAC GAAACAGAAG AAAGAGAACG TTTACAATTG 
CAGTTACATC AAGTAAAAAA ACAGTTAATG GATGTTGACA ATAGTCTAAA AGTAACAAGA 
CAAGAACTTG GAAGATTGCG GTCACGTGCA GATGAAGAGA ATGAACGGTG GAGAGTTAGA 
GAACAAGAAC TAGTAGTTCG TCTTGAGGAT AGTCGATGTC GTGAAAGAAA ACTCGAGGAT 
CAAAAACATA ACTTAGAAGT TTGTTTAGCT GATGCTTCTC AACAAATTCA AGAACTGAAG 
GCACGTCTTG GAGGTTCCGA AGGACGAGTT CGAGCTTTAG ATGCGCAATT ATCGCAATTA 
GAAATAACAA AAAAAGAAGT AGAACAAAAA TTAAGTAGCG TGGGATCGAC TTTACGTCGA 
ATTGCAGGTA TTCAAATGGA TGGAAGTGTT AATATACCCT TTAAATTATT GAGTCCATCA 
AGAAGGTGGA GTCCAGCAAG AGCTCAAGAT CATATTGATT CTAGTAGAGA TGTGATTCTT 
GATGTTGATC CTGAGATTAT TAGAAAAGGT GTACGATCTC TTATGCAACA AGTAGCACAA 
ATCGAACGTG AAAGAGATGA TTATAAAACT GAATTATGTA ATTTGAAAAA ACAATTGGAT 
GAAAATCAAG AGATTCAAAA TAGATCTGAT ATACAAATAA ACACTTTATT AACAAATATA 
AGAATGCTTC AGGATGAAAA GAATTCATTG GAGGTAAAAC TTTCTCAAAA ACAAAGTGGT 
TATGAAATGC AGTTAAATGC TTTACAACTA AAGTCAGAAG AATGCGAGCA ATTACGCGAG 
AAGATTATCA ATCTTGAATT AATGATTAGT AATAATTCTG AAGAAAAAAT ACAATTTGAG 
GAAAAACTAG ATAAATTAAA ACAAGTTTTA AACAAAGTAG AAAATGAAAA ACGTAATTTA 
CAAGAAGAAC TTAGTAAAAG CGAATCCCGC GCTACGAAAT TAGAATTACA AAGAATGTCA 
TTAGAAGGTG ACCTTCAAAG ATTGCAAATG ATGTTCCAAG AAAAAGATGC AAATATTCAT 
AAATTACAAG AACGAAATGA TACACAAAAT CGAACAATGA CAACTTTAGA AGAACGTTGT 
ACTTCATTGA AATCTATGGT GGAACAATTA AATCTTGCAT TGGAAAAAGC ATCTACTACA 
GAAAATGAAT TAAAGAATGA AATTAATTCA ATGCAACATA ATATTATGGA ATTAACAACT 
ACTTTGCAAA CTTCTAATGA AAAAAATAAA CAGTTACAAA AACAAATTTC AAATGCTGAA 
AATGAACGTA GAATCTTATC TGAACGTATA GAATCAATGC AACAATCTTT AAATGATCTC 
AAACATACAA ATCAAACATT AACAGATCAA ATTACACGTC TTCAAAATGA ATTGGCAAAT 
AATGAAGTAC AACGTTGTGC CTTAGAATCT CAATTAAGAA TAGTTGCCTA TCCAACACAA 
GAAGAAAATA TAAATAAGGA TGAAGAATTA TTACGACAGT TACAAATAGC ACAAAGAGAG 
AGAAGTGAAA TGAGAGGAAA AATGGAAGCA CTTAATGATA AAATGAAATT ATTGGAAGCT 
GATAAACGTA ATTTAGAACG ACAACTATCG TTATTTAAAT CAACTAATCG TAGCAAAAGT 
TATGAACGTT ATGAGAAAGC ACATACAGAA TTATTAGGCA CAAGTTTCGA TATAGATCAT 
TATGAACAAG AAAATAGAGA ATTAAGATTG AAAGTTCGTA GATTAGAAAC ACAACTTGCA 
GAGAAGGAAG CTGAACTTAT AAGATTGAAA TCAAGTTACA CTCATACACA TTCCGTATTT 
GATTTTAATC GAGATAGAAC GGGCGAAATT GAAAGACTTA GAGCAGCTCA ATTACAAGCT 
GAAAAATTGT TAGAAGCAAG AGAACAAAGT CATCGTCAAC AAGTTTTGCG TTTGGAAAAT 
CAGATACAAT TGTTACGCGA GCAACTTAAT CAAGAAATAA AACGTCGACA ATTATATGTT 
TTACGAAGTT CAAGGGCAGG TAGAGAAATG CAACAATTAA GACAAGCCTT AGGCGATTCT 
TTAAGAACTG TAGCTCAAGA TCCATCATTA GACGCAGTAT TATTAGAACA TGAAGCTCGA 
AAATTGGATT CTACTTTGAC AAGTACTACC AGTTTACCAC CATCATTAGC TTTACCTCCG 
CCACCGTCTT ATGATAGATC TTCCACACCA GCACAACTCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006559452.1
CDS	86..6010
Translation	MSRPLPKNISQLSARGQMERRRPPFLRGPSRMREKIERGIGSKMDSTGSTLSGTGDCLSEEDLSPDALVRQNYELRHRLEEEAANYKRRLDTYRQAQQHQAALVSRLQAKVLQYKQKCSELENQMAESIVYDSNKVVSSAPPTTSVLDAAHQTLREIREEQIYDLDTALKKLSEERKRCEKLLQLNTTLKDQLEESHQTNEALTNDLQKLSNDWDILREELAIKEEEWREEEQAFNDYYTSEHNRLLILWRDVVSVKRLFADIKSSTERDLLKMKNNIISTFNDVSSACNNTGFAMRMQAAMHPMISQQVQQAQEQVTTDLKTELITLKQQYDVAQNEIRIKEEKINQLIRDVHSLEERCGEAETEIHRNTRLQDDIEILQSALRDIAHAVIQDAESREIESTQAPPHLHLSPTGPIPQKSPKRGTRSNTIPAFAESTISAVQAALHKYQLTIHELQVKLQTNKEQLQNTRKQCENAEENVKTLEKKAEELIIELDAVRLHCSQLNQEKDMLQKGLDTIRIEKNTLEKTRIELNNMMENLNNDCEKLQKANNKLQKICDNLEDEKLYLQSELNRLSKDVELRELNLRSEEDRCSKMREELLTLREELNKTYLSKDMLEQQKLETDGLISQIEKSKGDLELELERVLLEKSDVQEVLMKLETVCSNHEQEKQRLQEELKKVTEEKNKLANQCTDQQGDLGSLRKELLQAEQTRLDLESEKVTLNEKVKFLEIEKEKIEIELAQVTRERGDFNNQLSVLARKKETLNEELMRLKQRLEQANEMNGRINRNLEDLVKDNEEKQVLLETNEKEVQRLQEQLASMRSEKETLEGVLFDTQTSLENMHVKKTQLEKEQKELLIKQESLKGQVERLMKDLENSEKRIHEVKQTLTQQSGDQEAEFQQIISNVKKHSEENIKKLNEEKEQIKINLEKRLQQSLLQVTGEKDNEINQLQQRIDEMQQHIENLCQQHEEVLLRAENDKQQALLIAHHDQQALMEKLETVLHEMEEEKNNVERVKREAAVKTEQERNNINQLRDELNRLKTKLDETRLKTDEEKIKLDLKIEELWKERELAQRESEELQVQLHMTEDKVDSLQNQLHETIRKLKDAENLNETLRKELVDIRRQLGDSTYEKEKYNSSNKELREHVKRIESEKREQNRILEESYQKISALEDMKVNVDAERSRLQAQIRDMEKEMLQLQKQLHFTQDELQKSHQSNAQAQNDEKELQARLTNETEERERLQLQLHQVKKQLMDVDNSLKVTRQELGRLRSRADEENERWRVREQELVVRLEDSRCRERKLEDQKHNLEVCLADASQQIQELKARLGGSEGRVRALDAQLSQLEITKKEVEQKLSSVGSTLRRIAGIQMDGSVNIPFKLLSPSRRWSPARAQDHIDSSRDVILDVDPEIIRKGVRSLMQQVAQIERERDDYKTELCNLKKQLDENQEIQNRSDIQINTLLTNIRMLQDEKNSLEVKLSQKQSGYEMQLNALQLKSEECEQLREKIINLELMISNNSEEKIQFEEKLDKLKQVLNKVENEKRNLQEELSKSESRATKLELQRMSLEGDLQRLQMMFQEKDANIHKLQERNDTQNRTMTTLEERCTSLKSMVEQLNLALEKASTTENELKNEINSMQHNIMELTTTLQTSNEKNKQLQKQISNAENERRILSERIESMQQSLNDLKHTNQTLTDQITRLQNELANNEVQRCALESQLRIVAYPTQEENINKDEELLRQLQIAQRERSEMRGKMEALNDKMKLLEADKRNLERQLSLFKSTNRSKSYERYEKAHTELLGTSFDIDHYEQENRELRLKVRRLETQLAEKEAELIRLKSSYTHTHSVFDFNRDRTGEIERLRAAQLQAEKLLEAREQSHRQQVLRLENQIQLLREQLNQEIKRRQLYVLRSSRAGREMQQLRQALGDSLRTVAQDPSLDAVLLEHEARKLDSTLTSTTSLPPSLALPPPPSYDRSSTPAQLK

Target ORF information:

RefSeq Version	XM_006559389.3
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera rootletin (LOC552019), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006559389.3

ORF Insert Sequence:

ATGTCACGGC CATTGCCTAA AAATATTTCT CAATTATCAG CAAGAGGACA GATGGAGAGG 
CGGCGACCAC CGTTTCTCAG AGGCCCTAGT AGAATGAGAG AGAAAATAGA AAGAGGCATA 
GGCAGTAAAA TGGATTCTAC AGGAAGTACA CTAAGTGGAA CTGGAGATTG TTTAAGTGAA 
GAAGATCTTT CACCAGATGC ATTGGTTAGA CAAAACTATG AATTACGTCA TCGTCTTGAA 
GAAGAAGCAG CCAATTATAA AAGACGTTTG GATACATATA GACAAGCACA ACAACATCAG 
GCTGCTCTTG TTTCACGTTT ACAAGCCAAA GTATTGCAAT ATAAACAAAA ATGTTCAGAG 
TTAGAAAATC AAATGGCAGA ATCTATAGTA TATGATTCTA ATAAAGTTGT AAGTTCTGCA 
CCTCCAACAA CTTCTGTTTT AGATGCAGCT CATCAAACAC TAAGAGAAAT ACGTGAAGAA 
CAAATATATG ATTTAGATAC TGCTCTAAAA AAACTAAGTG AAGAACGTAA AAGATGTGAA 
AAATTGCTGC AATTAAATAC AACATTGAAA GATCAGTTAG AAGAATCTCA TCAAACAAAT 
GAAGCACTTA CAAATGATCT ACAAAAATTA AGTAATGATT GGGATATTTT AAGAGAAGAA 
TTAGCTATCA AAGAAGAAGA GTGGAGAGAA GAAGAGCAAG CTTTTAATGA TTATTATACT 
TCAGAACATA ACAGATTATT AATTCTTTGG CGCGATGTTG TTTCTGTAAA ACGATTATTT 
GCAGATATAA AATCTAGTAC AGAAAGGGAT CTATTAAAAA TGAAAAATAA CATTATTTCT 
ACTTTTAATG ATGTTTCATC AGCTTGTAAT AATACAGGAT TTGCTATGAG AATGCAAGCA 
GCTATGCATC CAATGATATC TCAACAAGTT CAACAGGCAC AAGAACAGGT AACAACTGAT 
TTAAAAACAG AATTAATAAC ACTTAAACAA CAGTATGATG TAGCTCAAAA TGAAATTCGT 
ATAAAGGAAG AAAAAATCAA TCAACTTATT CGTGATGTTC ACAGTTTGGA AGAAAGATGT 
GGAGAAGCAG AAACAGAAAT TCATCGCAAT ACACGTTTGC AAGATGATAT AGAAATTTTA 
CAATCTGCAT TGAGAGATAT AGCACATGCT GTAATTCAAG ATGCAGAAAG TCGCGAAATT 
GAATCAACTC AAGCACCTCC TCATCTTCAT TTATCACCTA CTGGACCAAT TCCTCAAAAA 
TCACCAAAAA GAGGTACAAG AAGCAACACC ATTCCAGCTT TTGCTGAAAG TACTATAAGT 
GCTGTACAAG CAGCTCTCCA TAAATATCAA TTGACTATAC ATGAACTTCA GGTGAAATTG 
CAAACTAATA AAGAACAACT ACAAAATACT CGCAAACAAT GCGAAAATGC GGAAGAGAAT 
GTTAAAACTC TAGAGAAAAA AGCAGAAGAA CTGATTATTG AATTAGATGC AGTTCGATTG 
CACTGTTCAC AACTTAATCA AGAAAAAGAT ATGTTACAAA AAGGACTTGA TACTATAAGA 
ATAGAAAAAA ATACTCTTGA AAAAACCAGG ATAGAACTTA ATAATATGAT GGAGAATTTA 
AACAATGATT GCGAAAAATT GCAAAAAGCT AACAACAAAT TGCAAAAAAT TTGCGACAAT 
TTAGAAGATG AAAAATTATA TCTTCAAAGT GAACTCAATA GATTATCTAA AGATGTAGAA 
TTGAGGGAAT TAAATTTGCG TTCAGAAGAA GATAGGTGCA GTAAAATGAG AGAGGAATTA 
TTAACTCTAC GTGAAGAATT GAATAAAACA TATCTCAGTA AAGATATGTT AGAACAACAA 
AAGTTAGAAA CAGATGGATT AATTTCACAA ATTGAAAAAA GCAAAGGTGA TTTAGAATTA 
GAATTGGAAC GCGTATTATT AGAAAAATCA GACGTGCAAG AAGTTTTAAT GAAACTAGAA 
ACAGTTTGCA GCAATCATGA ACAAGAGAAA CAACGATTAC AAGAAGAATT AAAAAAGGTA 
ACAGAAGAAA AAAATAAGTT AGCAAATCAA TGTACGGATC AACAAGGTGA TCTGGGTTCC 
TTAAGAAAAG AATTACTTCA AGCAGAACAA ACTAGACTTG ATTTAGAATC AGAAAAAGTA 
ACTTTGAATG AAAAAGTGAA ATTTCTGGAG ATCGAAAAGG AGAAGATTGA AATAGAATTG 
GCACAAGTTA CGCGCGAACG TGGAGATTTC AATAATCAAT TATCAGTTTT AGCACGAAAG 
AAAGAAACAT TAAACGAAGA ATTGATGAGA CTCAAACAAA GATTAGAACA GGCAAACGAA 
ATGAATGGAA GAATAAATCG GAATTTAGAA GATCTTGTAA AAGATAATGA AGAAAAACAA 
GTTCTTTTAG AAACTAATGA AAAGGAAGTA CAACGATTAC AAGAGCAACT TGCTTCAATG 
CGTAGTGAGA AAGAGACATT AGAAGGTGTT TTATTTGATA CACAAACTAG TCTAGAAAAT 
ATGCATGTGA AAAAGACTCA ATTAGAAAAG GAACAGAAAG AACTTTTAAT AAAACAAGAA 
AGCTTAAAAG GACAAGTAGA AAGATTAATG AAAGATTTGG AGAATAGCGA AAAACGAATT 
CATGAAGTAA AGCAAACTTT AACGCAACAA AGTGGCGATC AAGAAGCCGA ATTTCAACAA 
ATTATTTCTA ACGTGAAAAA ACATAGTGAA GAAAACATAA AAAAATTAAA TGAAGAAAAA 
GAACAAATAA AGATAAATTT AGAAAAACGA CTTCAGCAAT CTCTCTTGCA AGTTACTGGA 
GAAAAAGATA ATGAAATAAA TCAATTGCAG CAAAGAATAG ATGAAATGCA ACAACATATA 
GAAAATTTGT GTCAGCAACA TGAAGAAGTT CTTCTTAGAG CAGAAAATGA TAAACAGCAA 
GCTCTTCTTA TAGCTCATCA TGATCAGCAA GCATTAATGG AAAAACTTGA AACTGTTTTG 
CATGAAATGG AAGAAGAGAA AAATAATGTA GAACGAGTCA AACGAGAAGC TGCAGTAAAA 
ACTGAACAAG AGCGTAATAA TATAAATCAA TTACGTGATG AATTAAATCG TCTTAAAACA 
AAATTAGATG AAACTAGATT AAAAACTGAT GAAGAAAAAA TAAAACTTGA TCTAAAAATA 
GAAGAACTCT GGAAAGAACG TGAATTAGCA CAAAGAGAAT CCGAAGAATT ACAAGTTCAA 
TTACATATGA CAGAAGATAA AGTTGATAGT TTACAAAATC AATTGCATGA AACTATTAGA 
AAACTTAAAG ATGCTGAAAA TCTTAACGAA ACATTACGCA AAGAATTAGT AGATATTAGA 
AGACAATTAG GTGATTCCAC ATATGAAAAA GAAAAGTATA ATAGTAGTAA TAAAGAATTA 
CGCGAGCATG TAAAACGCAT TGAAAGCGAA AAAAGAGAAC AAAATAGAAT ATTAGAAGAA 
TCATATCAAA AAATTTCAGC ACTGGAAGAT ATGAAAGTAA ATGTAGATGC AGAGAGATCT 
CGTCTTCAAG CACAAATTCG TGATATGGAG AAAGAAATGT TGCAATTACA AAAACAACTT 
CATTTTACTC AAGATGAATT ACAAAAATCA CATCAAAGTA ATGCTCAAGC ACAAAATGAT 
GAAAAAGAAT TGCAAGCTCG ACTAACTAAC GAAACAGAAG AAAGAGAACG TTTACAATTG 
CAGTTACATC AAGTAAAAAA ACAGTTAATG GATGTTGACA ATAGTCTAAA AGTAACAAGA 
CAAGAACTTG GAAGATTGCG GTCACGTGCA GATGAAGAGA ATGAACGGTG GAGAGTTAGA 
GAACAAGAAC TAGTAGTTCG TCTTGAGGAT AGTCGATGTC GTGAAAGAAA ACTCGAGGAT 
CAAAAACATA ACTTAGAAGT TTGTTTAGCT GATGCTTCTC AACAAATTCA AGAACTGAAG 
GCACGTCTTG GAGGTTCCGA AGGACGAGTT CGAGCTTTAG ATGCGCAATT ATCGCAATTA 
GAAATAACAA AAAAAGAAGT AGAACAAAAA TTAAGTAGCG TGGGATCGAC TTTACGTCGA 
ATTGCAGGTA TTCAAATGGA TGGAAGTGTT AATATACCCT TTAAATTATT GAGTCCATCA 
AGAAGGTGGA GTCCAGCAAG AGCTCAAGAT CATATTGATT CTAGTAGAGA TGTGATTCTT 
GATGTTGATC CTGAGATTAT TAGAAAAGGT GTACGATCTC TTATGCAACA AGTAGCACAA 
ATCGAACGTG AAAGAGATGA TTATAAAACT GAATTATGTA ATTTGAAAAA ACAATTGGAT 
GAAAATCAAG AGATTCAAAA TAGATCTGAT ATACAAATAA ACACTTTATT AACAAATATA 
AGAATGCTTC AGGATGAAAA GAATTCATTG GAGGTAAAAC TTTCTCAAAA ACAAAGTGGT 
TATGAAATGC AGTTAAATGC TTTACAACTA AAGTCAGAAG AATGCGAGCA ATTACGCGAG 
AAGATTATCA ATCTTGAATT AATGATTAGT AATAATTCTG AAGAAAAAAT ACAATTTGAG 
GAAAAACTAG ATAAATTAAA ACAAGTTTTA AACAAAGTAG AAAATGAAAA ACGTAATTTA 
CAAGAAGAAC TTAGTAAAAG CGAATCCCGC GCTACGAAAT TAGAATTACA AAGAATGTCA 
TTAGAAGGTG ACCTTCAAAG ATTGCAAATG ATGTTCCAAG AAAAAGATGC AAATATTCAT 
AAATTACAAG AACGAAATGA TACACAAAAT CGAACAATGA CAACTTTAGA AGAACGTTGT 
ACTTCATTGA AATCTATGGT GGAACAATTA AATCTTGCAT TGGAAAAAGC ATCTACTACA 
GAAAATGAAT TAAAGAATGA AATTAATTCA ATGCAACATA ATATTATGGA ATTAACAACT 
ACTTTGCAAA CTTCTAATGA AAAAAATAAA CAGTTACAAA AACAAATTTC AAATGCTGAA 
AATGAACGTA GAATCTTATC TGAACGTATA GAATCAATGC AACAATCTTT AAATGATCTC 
AAACATACAA ATCAAACATT AACAGATCAA ATTACACGTC TTCAAAATGA ATTGGCAAAT 
AATGAAGTAC AACGTTGTGC CTTAGAATCT CAATTAAGAA TAGTTGCCTA TCCAACACAA 
GAAGAAAATA TAAATAAGGA TGAAGAATTA TTACGACAGT TACAAATAGC ACAAAGAGAG 
AGAAGTGAAA TGAGAGGAAA AATGGAAGCA CTTAATGATA AAATGAAATT ATTGGAAGCT 
GATAAACGTA ATTTAGAACG ACAACTATCG TTATTTAAAT CAACTAATCG TAGCAAAAGT 
TATGAACGTT ATGAGAAAGC ACATACAGAA TTATTAGGCA CAAGTTTCGA TATAGATCAT 
TATGAACAAG AAAATAGAGA ATTAAGATTG AAAGTTCGTA GATTAGAAAC ACAACTTGCA 
GAGAAGGAAG CTGAACTTAT AAGATTGAAA TCAAGTTACA CTCATACACA TTCCGTATTT 
GATTTTAATC GAGATAGAAC GGGCGAAATT GAAAGACTTA GAGCAGCTCA ATTACAAGCT 
GAAAAATTGT TAGAAGCAAG AGAACAAAGT CATCGTCAAC AAGTTTTGCG TTTGGAAAAT 
CAGATACAAT TGTTACGCGA GCAACTTAAT CAAGAAATAA AACGTCGACA ATTATATGTT 
TTACGAAGTT CAAGGGCAGG TAGAGAAATG CAACAATTAA GACAAGCCTT AGGCGATTCT 
TTAAGAACTG TAGCTCAAGA TCCATCATTA GACGCAGTAT TATTAGAACA TGAAGCTCGA 
AAATTGGATT CTACTTTGAC AAGTACTACC AGTTTACCAC CATCATTAGC TTTACCTCCG 
CCACCGTCTT ATGATAGATC TTCCACACCA GCACAACTCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552019 ( XM_006559391.3 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006559454.1 Apis mellifera rootletin (LOC552019), transcript variant X3, mRNA.

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CloneID	OHd16378
Clone ID Related Accession (Same CDS sequence)	XM_006559391.3 , XM_006559391.2
Accession Version	XM_006559391.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	rootletin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_006559391.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAG AAAGAGGCAT AGGCAGTAAA ATGGATTCTA CAGGAAGTAC ACTAAGTGGA 
ACTGGAGATT GTTTAAGTGA AGAAGATCTT TCACCAGATG CATTGGTTAG ACAAAACTAT 
GAATTACGTC ATCGTCTTGA AGAAGAAGCA GCCAATTATA AAAGACGTTT GGATACATAT 
AGACAAGCAC AACAACATCA GGCTGCTCTT GTTTCACGTT TACAAGCCAA AGTATTGCAA 
TATAAACAAA AATGTTCAGA GTTAGAAAAT CAAATGGCAG AATCTATAGT ATATGATTCT 
AATAAAGTTG TAAGTTCTGC ACCTCCAACA ACTTCTGTTT TAGATGCAGC TCATCAAACA 
CTAAGAGAAA TACGTGAAGA ACAAATATAT GATTTAGATA CTGCTCTAAA AAAACTAAGT 
GAAGAACGTA AAAGATGTGA AAAATTGCTG CAATTAAATA CAACATTGAA AGATCAGTTA 
GAAGAATCTC ATCAAACAAA TGAAGCACTT ACAAATGATC TACAAAAATT AAGTAATGAT 
TGGGATATTT TAAGAGAAGA ATTAGCTATC AAAGAAGAAG AGTGGAGAGA AGAAGAGCAA 
GCTTTTAATG ATTATTATAC TTCAGAACAT AACAGATTAT TAATTCTTTG GCGCGATGTT 
GTTTCTGTAA AACGATTATT TGCAGATATA AAATCTAGTA CAGAAAGGGA TCTATTAAAA 
ATGAAAAATA ACATTATTTC TACTTTTAAT GATGTTTCAT CAGCTTGTAA TAATACAGGA 
TTTGCTATGA GAATGCAAGC AGCTATGCAT CCAATGATAT CTCAACAAGT TCAACAGGCA 
CAAGAACAGG TAACAACTGA TTTAAAAACA GAATTAATAA CACTTAAACA ACAGTATGAT 
GTAGCTCAAA ATGAAATTCG TATAAAGGAA GAAAAAATCA ATCAACTTAT TCGTGATGTT 
CACAGTTTGG AAGAAAGATG TGGAGAAGCA GAAACAGAAA TTCATCGCAA TACACGTTTG 
CAAGATGATA TAGAAATTTT ACAATCTGCA TTGAGAGATA TAGCACATGC TGTAATTCAA 
GATGCAGAAA GTCGCGAAAT TGAATCAACT CAAGCACCTC CTCATCTTCA TTTATCACCT 
ACTGGACCAA TTCCTCAAAA ATCACCAAAA AGAGGTACAA GAAGCAACAC CATTCCAGCT 
TTTGCTGAAA GTACTATAAG TGCTGTACAA GCAGCTCTCC ATAAATATCA ATTGACTATA 
CATGAACTTC AGGTGAAATT GCAAACTAAT AAAGAACAAC TACAAAATAC TCGCAAACAA 
TGCGAAAATG CGGAAGAGAA TGTTAAAACT CTAGAGAAAA AAGCAGAAGA ACTGATTATT 
GAATTAGATG CAGTTCGATT GCACTGTTCA CAACTTAATC AAGAAAAAGA TATGTTACAA 
AAAGGACTTG ATACTATAAG AATAGAAAAA AATACTCTTG AAAAAACCAG GATAGAACTT 
AATAATATGA TGGAGAATTT AAACAATGAT TGCGAAAAAT TGCAAAAAGC TAACAACAAA 
TTGCAAAAAA TTTGCGACAA TTTAGAAGAT GAAAAATTAT ATCTTCAAAG TGAACTCAAT 
AGATTATCTA AAGATGTAGA ATTGAGGGAA TTAAATTTGC GTTCAGAAGA AGATAGGTGC 
AGTAAAATGA GAGAGGAATT ATTAACTCTA CGTGAAGAAT TGAATAAAAC ATATCTCAGT 
AAAGATATGT TAGAACAACA AAAGTTAGAA ACAGATGGAT TAATTTCACA AATTGAAAAA 
AGCAAAGGTG ATTTAGAATT AGAATTGGAA CGCGTATTAT TAGAAAAATC AGACGTGCAA 
GAAGTTTTAA TGAAACTAGA AACAGTTTGC AGCAATCATG AACAAGAGAA ACAACGATTA 
CAAGAAGAAT TAAAAAAGGT AACAGAAGAA AAAAATAAGT TAGCAAATCA ATGTACGGAT 
CAACAAGGTG ATCTGGGTTC CTTAAGAAAA GAATTACTTC AAGCAGAACA AACTAGACTT 
GATTTAGAAT CAGAAAAAGT AACTTTGAAT GAAAAAGTGA AATTTCTGGA GATCGAAAAG 
GAGAAGATTG AAATAGAATT GGCACAAGTT ACGCGCGAAC GTGGAGATTT CAATAATCAA 
TTATCAGTTT TAGCACGAAA GAAAGAAACA TTAAACGAAG AATTGATGAG ACTCAAACAA 
AGATTAGAAC AGGCAAACGA AATGAATGGA AGAATAAATC GGAATTTAGA AGATCTTGTA 
AAAGATAATG AAGAAAAACA AGTTCTTTTA GAAACTAATG AAAAGGAAGT ACAACGATTA 
CAAGAGCAAC TTGCTTCAAT GCGTAGTGAG AAAGAGACAT TAGAAGGTGT TTTATTTGAT 
ACACAAACTA GTCTAGAAAA TATGCATGTG AAAAAGACTC AATTAGAAAA GGAACAGAAA 
GAACTTTTAA TAAAACAAGA AAGCTTAAAA GGACAAGTAG AAAGATTAAT GAAAGATTTG 
GAGAATAGCG AAAAACGAAT TCATGAAGTA AAGCAAACTT TAACGCAACA AAGTGGCGAT 
CAAGAAGCCG AATTTCAACA AATTATTTCT AACGTGAAAA AACATAGTGA AGAAAACATA 
AAAAAATTAA ATGAAGAAAA AGAACAAATA AAGATAAATT TAGAAAAACG ACTTCAGCAA 
TCTCTCTTGC AAGTTACTGG AGAAAAAGAT AATGAAATAA ATCAATTGCA GCAAAGAATA 
GATGAAATGC AACAACATAT AGAAAATTTG TGTCAGCAAC ATGAAGAAGT TCTTCTTAGA 
GCAGAAAATG ATAAACAGCA AGCTCTTCTT ATAGCTCATC ATGATCAGCA AGCATTAATG 
GAAAAACTTG AAACTGTTTT GCATGAAATG GAAGAAGAGA AAAATAATGT AGAACGAGTC 
AAACGAGAAG CTGCAGTAAA AACTGAACAA GAGCGTAATA ATATAAATCA ATTACGTGAT 
GAATTAAATC GTCTTAAAAC AAAATTAGAT GAAACTAGAT TAAAAACTGA TGAAGAAAAA 
ATAAAACTTG ATCTAAAAAT AGAAGAACTC TGGAAAGAAC GTGAATTAGC ACAAAGAGAA 
TCCGAAGAAT TACAAGTTCA ATTACATATG ACAGAAGATA AAGTTGATAG TTTACAAAAT 
CAATTGCATG AAACTATTAG AAAACTTAAA GATGCTGAAA ATCTTAACGA AACATTACGC 
AAAGAATTAG TAGATATTAG AAGACAATTA GGTGATTCCA CATATGAAAA AGAAAAGTAT 
AATAGTAGTA ATAAAGAATT ACGCGAGCAT GTAAAACGCA TTGAAAGCGA AAAAAGAGAA 
CAAAATAGAA TATTAGAAGA ATCATATCAA AAAATTTCAG CACTGGAAGA TATGAAAGTA 
AATGTAGATG CAGAGAGATC TCGTCTTCAA GCACAAATTC GTGATATGGA GAAAGAAATG 
TTGCAATTAC AAAAACAACT TCATTTTACT CAAGATGAAT TACAAAAATC ACATCAAAGT 
AATGCTCAAG CACAAAATGA TGAAAAAGAA TTGCAAGCTC GACTAACTAA CGAAACAGAA 
GAAAGAGAAC GTTTACAATT GCAGTTACAT CAAGTAAAAA AACAGTTAAT GGATGTTGAC 
AATAGTCTAA AAGTAACAAG ACAAGAACTT GGAAGATTGC GGTCACGTGC AGATGAAGAG 
AATGAACGGT GGAGAGTTAG AGAACAAGAA CTAGTAGTTC GTCTTGAGGA TAGTCGATGT 
CGTGAAAGAA AACTCGAGGA TCAAAAACAT AACTTAGAAG TTTGTTTAGC TGATGCTTCT 
CAACAAATTC AAGAACTGAA GGCACGTCTT GGAGGTTCCG AAGGACGAGT TCGAGCTTTA 
GATGCGCAAT TATCGCAATT AGAAATAACA AAAAAAGAAG TAGAACAAAA ATTAAGTAGC 
GTGGGATCGA CTTTACGTCG AATTGCAGGT ATTCAAATGG ATGGAAGTGT TAATATACCC 
TTTAAATTAT TGAGTCCATC AAGAAGGTGG AGTCCAGCAA GAGCTCAAGA TCATATTGAT 
TCTAGTAGAG ATGTGATTCT TGATGTTGAT CCTGAGATTA TTAGAAAAGG TGTACGATCT 
CTTATGCAAC AAGTAGCACA AATCGAACGT GAAAGAGATG ATTATAAAAC TGAATTATGT 
AATTTGAAAA AACAATTGGA TGAAAATCAA GAGATTCAAA ATAGATCTGA TATACAAATA 
AACACTTTAT TAACAAATAT AAGAATGCTT CAGGATGAAA AGAATTCATT GGAGGTAAAA 
CTTTCTCAAA AACAAAGTGG TTATGAAATG CAGTTAAATG CTTTACAACT AAAGTCAGAA 
GAATGCGAGC AATTACGCGA GAAGATTATC AATCTTGAAT TAATGATTAG TAATAATTCT 
GAAGAAAAAA TACAATTTGA GGAAAAACTA GATAAATTAA AACAAGTTTT AAACAAAGTA 
GAAAATGAAA AACGTAATTT ACAAGAAGAA CTTAGTAAAA GCGAATCCCG CGCTACGAAA 
TTAGAATTAC AAAGAATGTC ATTAGAAGGT GACCTTCAAA GATTGCAAAT GATGTTCCAA 
GAAAAAGATG CAAATATTCA TAAATTACAA GAACGAAATG ATACACAAAA TCGAACAATG 
ACAACTTTAG AAGAACGTTG TACTTCATTG AAATCTATGG TGGAACAATT AAATCTTGCA 
TTGGAAAAAG CATCTACTAC AGAAAATGAA TTAAAGAATG AAATTAATTC AATGCAACAT 
AATATTATGG AATTAACAAC TACTTTGCAA ACTTCTAATG AAAAAAATAA ACAGTTACAA 
AAACAAATTT CAAATGCTGA AAATGAACGT AGAATCTTAT CTGAACGTAT AGAATCAATG 
CAACAATCTT TAAATGATCT CAAACATACA AATCAAACAT TAACAGATCA AATTACACGT 
CTTCAAAATG AATTGGCAAA TAATGAAGTA CAACGTTGTG CCTTAGAATC TCAATTAAGA 
ATAGTTGCCT ATCCAACACA AGAAGAAAAT ATAAATAAGG ATGAAGAATT ATTACGACAG 
TTACAAATAG CACAAAGAGA GAGAAGTGAA ATGAGAGGAA AAATGGAAGC ACTTAATGAT 
AAAATGAAAT TATTGGAAGC TGATAAACGT AATTTAGAAC GACAACTATC GTTATTTAAA 
TCAACTAATC GTAGCAAAAG TTATGAACGT TATGAGAAAG CACATACAGA ATTATTAGGC 
ACAAGTTTCG ATATAGATCA TTATGAACAA GAAAATAGAG AATTAAGATT GAAAGTTCGT 
AGATTAGAAA CACAACTTGC AGAGAAGGAA GCTGAACTTA TAAGATTGAA ATCAAGTTAC 
ACTCATACAC ATTCCGTATT TGATTTTAAT CGAGATAGAA CGGGCGAAAT TGAAAGACTT 
AGAGCAGCTC AATTACAAGC TGAAAAATTG TTAGAAGCAA GAGAACAAAG TCATCGTCAA 
CAAGTTTTGC GTTTGGAAAA TCAGATACAA TTGTTACGCG AGCAACTTAA TCAAGAAATA 
AAACGTCGAC AATTATATGT TTTACGAAGT TCAAGGGCAG GTAGAGAAAT GCAACAATTA 
AGACAAGCCT TAGGCGATTC TTTAAGAACT GTAGCTCAAG ATCCATCATT AGACGCAGTA 
TTATTAGAAC ATGAAGCTCG AAAATTGGAT TCTACTTTGA CAAGTACTAC CAGTTTACCA 
CCATCATTAG CTTTACCTCC GCCACCGTCT TATGATAGAT CTTCCACACC AGCACAACTC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006559454.1
CDS	29..5854
Translation	MKIERGIGSKMDSTGSTLSGTGDCLSEEDLSPDALVRQNYELRHRLEEEAANYKRRLDTYRQAQQHQAALVSRLQAKVLQYKQKCSELENQMAESIVYDSNKVVSSAPPTTSVLDAAHQTLREIREEQIYDLDTALKKLSEERKRCEKLLQLNTTLKDQLEESHQTNEALTNDLQKLSNDWDILREELAIKEEEWREEEQAFNDYYTSEHNRLLILWRDVVSVKRLFADIKSSTERDLLKMKNNIISTFNDVSSACNNTGFAMRMQAAMHPMISQQVQQAQEQVTTDLKTELITLKQQYDVAQNEIRIKEEKINQLIRDVHSLEERCGEAETEIHRNTRLQDDIEILQSALRDIAHAVIQDAESREIESTQAPPHLHLSPTGPIPQKSPKRGTRSNTIPAFAESTISAVQAALHKYQLTIHELQVKLQTNKEQLQNTRKQCENAEENVKTLEKKAEELIIELDAVRLHCSQLNQEKDMLQKGLDTIRIEKNTLEKTRIELNNMMENLNNDCEKLQKANNKLQKICDNLEDEKLYLQSELNRLSKDVELRELNLRSEEDRCSKMREELLTLREELNKTYLSKDMLEQQKLETDGLISQIEKSKGDLELELERVLLEKSDVQEVLMKLETVCSNHEQEKQRLQEELKKVTEEKNKLANQCTDQQGDLGSLRKELLQAEQTRLDLESEKVTLNEKVKFLEIEKEKIEIELAQVTRERGDFNNQLSVLARKKETLNEELMRLKQRLEQANEMNGRINRNLEDLVKDNEEKQVLLETNEKEVQRLQEQLASMRSEKETLEGVLFDTQTSLENMHVKKTQLEKEQKELLIKQESLKGQVERLMKDLENSEKRIHEVKQTLTQQSGDQEAEFQQIISNVKKHSEENIKKLNEEKEQIKINLEKRLQQSLLQVTGEKDNEINQLQQRIDEMQQHIENLCQQHEEVLLRAENDKQQALLIAHHDQQALMEKLETVLHEMEEEKNNVERVKREAAVKTEQERNNINQLRDELNRLKTKLDETRLKTDEEKIKLDLKIEELWKERELAQRESEELQVQLHMTEDKVDSLQNQLHETIRKLKDAENLNETLRKELVDIRRQLGDSTYEKEKYNSSNKELREHVKRIESEKREQNRILEESYQKISALEDMKVNVDAERSRLQAQIRDMEKEMLQLQKQLHFTQDELQKSHQSNAQAQNDEKELQARLTNETEERERLQLQLHQVKKQLMDVDNSLKVTRQELGRLRSRADEENERWRVREQELVVRLEDSRCRERKLEDQKHNLEVCLADASQQIQELKARLGGSEGRVRALDAQLSQLEITKKEVEQKLSSVGSTLRRIAGIQMDGSVNIPFKLLSPSRRWSPARAQDHIDSSRDVILDVDPEIIRKGVRSLMQQVAQIERERDDYKTELCNLKKQLDENQEIQNRSDIQINTLLTNIRMLQDEKNSLEVKLSQKQSGYEMQLNALQLKSEECEQLREKIINLELMISNNSEEKIQFEEKLDKLKQVLNKVENEKRNLQEELSKSESRATKLELQRMSLEGDLQRLQMMFQEKDANIHKLQERNDTQNRTMTTLEERCTSLKSMVEQLNLALEKASTTENELKNEINSMQHNIMELTTTLQTSNEKNKQLQKQISNAENERRILSERIESMQQSLNDLKHTNQTLTDQITRLQNELANNEVQRCALESQLRIVAYPTQEENINKDEELLRQLQIAQRERSEMRGKMEALNDKMKLLEADKRNLERQLSLFKSTNRSKSYERYEKAHTELLGTSFDIDHYEQENRELRLKVRRLETQLAEKEAELIRLKSSYTHTHSVFDFNRDRTGEIERLRAAQLQAEKLLEAREQSHRQQVLRLENQIQLLREQLNQEIKRRQLYVLRSSRAGREMQQLRQALGDSLRTVAQDPSLDAVLLEHEARKLDSTLTSTTSLPPSLALPPPPSYDRSSTPAQLK

Target ORF information:

RefSeq Version	XM_006559391.3
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera rootletin (LOC552019), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006559391.3

ORF Insert Sequence:

ATGAAAATAG AAAGAGGCAT AGGCAGTAAA ATGGATTCTA CAGGAAGTAC ACTAAGTGGA 
ACTGGAGATT GTTTAAGTGA AGAAGATCTT TCACCAGATG CATTGGTTAG ACAAAACTAT 
GAATTACGTC ATCGTCTTGA AGAAGAAGCA GCCAATTATA AAAGACGTTT GGATACATAT 
AGACAAGCAC AACAACATCA GGCTGCTCTT GTTTCACGTT TACAAGCCAA AGTATTGCAA 
TATAAACAAA AATGTTCAGA GTTAGAAAAT CAAATGGCAG AATCTATAGT ATATGATTCT 
AATAAAGTTG TAAGTTCTGC ACCTCCAACA ACTTCTGTTT TAGATGCAGC TCATCAAACA 
CTAAGAGAAA TACGTGAAGA ACAAATATAT GATTTAGATA CTGCTCTAAA AAAACTAAGT 
GAAGAACGTA AAAGATGTGA AAAATTGCTG CAATTAAATA CAACATTGAA AGATCAGTTA 
GAAGAATCTC ATCAAACAAA TGAAGCACTT ACAAATGATC TACAAAAATT AAGTAATGAT 
TGGGATATTT TAAGAGAAGA ATTAGCTATC AAAGAAGAAG AGTGGAGAGA AGAAGAGCAA 
GCTTTTAATG ATTATTATAC TTCAGAACAT AACAGATTAT TAATTCTTTG GCGCGATGTT 
GTTTCTGTAA AACGATTATT TGCAGATATA AAATCTAGTA CAGAAAGGGA TCTATTAAAA 
ATGAAAAATA ACATTATTTC TACTTTTAAT GATGTTTCAT CAGCTTGTAA TAATACAGGA 
TTTGCTATGA GAATGCAAGC AGCTATGCAT CCAATGATAT CTCAACAAGT TCAACAGGCA 
CAAGAACAGG TAACAACTGA TTTAAAAACA GAATTAATAA CACTTAAACA ACAGTATGAT 
GTAGCTCAAA ATGAAATTCG TATAAAGGAA GAAAAAATCA ATCAACTTAT TCGTGATGTT 
CACAGTTTGG AAGAAAGATG TGGAGAAGCA GAAACAGAAA TTCATCGCAA TACACGTTTG 
CAAGATGATA TAGAAATTTT ACAATCTGCA TTGAGAGATA TAGCACATGC TGTAATTCAA 
GATGCAGAAA GTCGCGAAAT TGAATCAACT CAAGCACCTC CTCATCTTCA TTTATCACCT 
ACTGGACCAA TTCCTCAAAA ATCACCAAAA AGAGGTACAA GAAGCAACAC CATTCCAGCT 
TTTGCTGAAA GTACTATAAG TGCTGTACAA GCAGCTCTCC ATAAATATCA ATTGACTATA 
CATGAACTTC AGGTGAAATT GCAAACTAAT AAAGAACAAC TACAAAATAC TCGCAAACAA 
TGCGAAAATG CGGAAGAGAA TGTTAAAACT CTAGAGAAAA AAGCAGAAGA ACTGATTATT 
GAATTAGATG CAGTTCGATT GCACTGTTCA CAACTTAATC AAGAAAAAGA TATGTTACAA 
AAAGGACTTG ATACTATAAG AATAGAAAAA AATACTCTTG AAAAAACCAG GATAGAACTT 
AATAATATGA TGGAGAATTT AAACAATGAT TGCGAAAAAT TGCAAAAAGC TAACAACAAA 
TTGCAAAAAA TTTGCGACAA TTTAGAAGAT GAAAAATTAT ATCTTCAAAG TGAACTCAAT 
AGATTATCTA AAGATGTAGA ATTGAGGGAA TTAAATTTGC GTTCAGAAGA AGATAGGTGC 
AGTAAAATGA GAGAGGAATT ATTAACTCTA CGTGAAGAAT TGAATAAAAC ATATCTCAGT 
AAAGATATGT TAGAACAACA AAAGTTAGAA ACAGATGGAT TAATTTCACA AATTGAAAAA 
AGCAAAGGTG ATTTAGAATT AGAATTGGAA CGCGTATTAT TAGAAAAATC AGACGTGCAA 
GAAGTTTTAA TGAAACTAGA AACAGTTTGC AGCAATCATG AACAAGAGAA ACAACGATTA 
CAAGAAGAAT TAAAAAAGGT AACAGAAGAA AAAAATAAGT TAGCAAATCA ATGTACGGAT 
CAACAAGGTG ATCTGGGTTC CTTAAGAAAA GAATTACTTC AAGCAGAACA AACTAGACTT 
GATTTAGAAT CAGAAAAAGT AACTTTGAAT GAAAAAGTGA AATTTCTGGA GATCGAAAAG 
GAGAAGATTG AAATAGAATT GGCACAAGTT ACGCGCGAAC GTGGAGATTT CAATAATCAA 
TTATCAGTTT TAGCACGAAA GAAAGAAACA TTAAACGAAG AATTGATGAG ACTCAAACAA 
AGATTAGAAC AGGCAAACGA AATGAATGGA AGAATAAATC GGAATTTAGA AGATCTTGTA 
AAAGATAATG AAGAAAAACA AGTTCTTTTA GAAACTAATG AAAAGGAAGT ACAACGATTA 
CAAGAGCAAC TTGCTTCAAT GCGTAGTGAG AAAGAGACAT TAGAAGGTGT TTTATTTGAT 
ACACAAACTA GTCTAGAAAA TATGCATGTG AAAAAGACTC AATTAGAAAA GGAACAGAAA 
GAACTTTTAA TAAAACAAGA AAGCTTAAAA GGACAAGTAG AAAGATTAAT GAAAGATTTG 
GAGAATAGCG AAAAACGAAT TCATGAAGTA AAGCAAACTT TAACGCAACA AAGTGGCGAT 
CAAGAAGCCG AATTTCAACA AATTATTTCT AACGTGAAAA AACATAGTGA AGAAAACATA 
AAAAAATTAA ATGAAGAAAA AGAACAAATA AAGATAAATT TAGAAAAACG ACTTCAGCAA 
TCTCTCTTGC AAGTTACTGG AGAAAAAGAT AATGAAATAA ATCAATTGCA GCAAAGAATA 
GATGAAATGC AACAACATAT AGAAAATTTG TGTCAGCAAC ATGAAGAAGT TCTTCTTAGA 
GCAGAAAATG ATAAACAGCA AGCTCTTCTT ATAGCTCATC ATGATCAGCA AGCATTAATG 
GAAAAACTTG AAACTGTTTT GCATGAAATG GAAGAAGAGA AAAATAATGT AGAACGAGTC 
AAACGAGAAG CTGCAGTAAA AACTGAACAA GAGCGTAATA ATATAAATCA ATTACGTGAT 
GAATTAAATC GTCTTAAAAC AAAATTAGAT GAAACTAGAT TAAAAACTGA TGAAGAAAAA 
ATAAAACTTG ATCTAAAAAT AGAAGAACTC TGGAAAGAAC GTGAATTAGC ACAAAGAGAA 
TCCGAAGAAT TACAAGTTCA ATTACATATG ACAGAAGATA AAGTTGATAG TTTACAAAAT 
CAATTGCATG AAACTATTAG AAAACTTAAA GATGCTGAAA ATCTTAACGA AACATTACGC 
AAAGAATTAG TAGATATTAG AAGACAATTA GGTGATTCCA CATATGAAAA AGAAAAGTAT 
AATAGTAGTA ATAAAGAATT ACGCGAGCAT GTAAAACGCA TTGAAAGCGA AAAAAGAGAA 
CAAAATAGAA TATTAGAAGA ATCATATCAA AAAATTTCAG CACTGGAAGA TATGAAAGTA 
AATGTAGATG CAGAGAGATC TCGTCTTCAA GCACAAATTC GTGATATGGA GAAAGAAATG 
TTGCAATTAC AAAAACAACT TCATTTTACT CAAGATGAAT TACAAAAATC ACATCAAAGT 
AATGCTCAAG CACAAAATGA TGAAAAAGAA TTGCAAGCTC GACTAACTAA CGAAACAGAA 
GAAAGAGAAC GTTTACAATT GCAGTTACAT CAAGTAAAAA AACAGTTAAT GGATGTTGAC 
AATAGTCTAA AAGTAACAAG ACAAGAACTT GGAAGATTGC GGTCACGTGC AGATGAAGAG 
AATGAACGGT GGAGAGTTAG AGAACAAGAA CTAGTAGTTC GTCTTGAGGA TAGTCGATGT 
CGTGAAAGAA AACTCGAGGA TCAAAAACAT AACTTAGAAG TTTGTTTAGC TGATGCTTCT 
CAACAAATTC AAGAACTGAA GGCACGTCTT GGAGGTTCCG AAGGACGAGT TCGAGCTTTA 
GATGCGCAAT TATCGCAATT AGAAATAACA AAAAAAGAAG TAGAACAAAA ATTAAGTAGC 
GTGGGATCGA CTTTACGTCG AATTGCAGGT ATTCAAATGG ATGGAAGTGT TAATATACCC 
TTTAAATTAT TGAGTCCATC AAGAAGGTGG AGTCCAGCAA GAGCTCAAGA TCATATTGAT 
TCTAGTAGAG ATGTGATTCT TGATGTTGAT CCTGAGATTA TTAGAAAAGG TGTACGATCT 
CTTATGCAAC AAGTAGCACA AATCGAACGT GAAAGAGATG ATTATAAAAC TGAATTATGT 
AATTTGAAAA AACAATTGGA TGAAAATCAA GAGATTCAAA ATAGATCTGA TATACAAATA 
AACACTTTAT TAACAAATAT AAGAATGCTT CAGGATGAAA AGAATTCATT GGAGGTAAAA 
CTTTCTCAAA AACAAAGTGG TTATGAAATG CAGTTAAATG CTTTACAACT AAAGTCAGAA 
GAATGCGAGC AATTACGCGA GAAGATTATC AATCTTGAAT TAATGATTAG TAATAATTCT 
GAAGAAAAAA TACAATTTGA GGAAAAACTA GATAAATTAA AACAAGTTTT AAACAAAGTA 
GAAAATGAAA AACGTAATTT ACAAGAAGAA CTTAGTAAAA GCGAATCCCG CGCTACGAAA 
TTAGAATTAC AAAGAATGTC ATTAGAAGGT GACCTTCAAA GATTGCAAAT GATGTTCCAA 
GAAAAAGATG CAAATATTCA TAAATTACAA GAACGAAATG ATACACAAAA TCGAACAATG 
ACAACTTTAG AAGAACGTTG TACTTCATTG AAATCTATGG TGGAACAATT AAATCTTGCA 
TTGGAAAAAG CATCTACTAC AGAAAATGAA TTAAAGAATG AAATTAATTC AATGCAACAT 
AATATTATGG AATTAACAAC TACTTTGCAA ACTTCTAATG AAAAAAATAA ACAGTTACAA 
AAACAAATTT CAAATGCTGA AAATGAACGT AGAATCTTAT CTGAACGTAT AGAATCAATG 
CAACAATCTT TAAATGATCT CAAACATACA AATCAAACAT TAACAGATCA AATTACACGT 
CTTCAAAATG AATTGGCAAA TAATGAAGTA CAACGTTGTG CCTTAGAATC TCAATTAAGA 
ATAGTTGCCT ATCCAACACA AGAAGAAAAT ATAAATAAGG ATGAAGAATT ATTACGACAG 
TTACAAATAG CACAAAGAGA GAGAAGTGAA ATGAGAGGAA AAATGGAAGC ACTTAATGAT 
AAAATGAAAT TATTGGAAGC TGATAAACGT AATTTAGAAC GACAACTATC GTTATTTAAA 
TCAACTAATC GTAGCAAAAG TTATGAACGT TATGAGAAAG CACATACAGA ATTATTAGGC 
ACAAGTTTCG ATATAGATCA TTATGAACAA GAAAATAGAG AATTAAGATT GAAAGTTCGT 
AGATTAGAAA CACAACTTGC AGAGAAGGAA GCTGAACTTA TAAGATTGAA ATCAAGTTAC 
ACTCATACAC ATTCCGTATT TGATTTTAAT CGAGATAGAA CGGGCGAAAT TGAAAGACTT 
AGAGCAGCTC AATTACAAGC TGAAAAATTG TTAGAAGCAA GAGAACAAAG TCATCGTCAA 
CAAGTTTTGC GTTTGGAAAA TCAGATACAA TTGTTACGCG AGCAACTTAA TCAAGAAATA 
AAACGTCGAC AATTATATGT TTTACGAAGT TCAAGGGCAG GTAGAGAAAT GCAACAATTA 
AGACAAGCCT TAGGCGATTC TTTAAGAACT GTAGCTCAAG ATCCATCATT AGACGCAGTA 
TTATTAGAAC ATGAAGCTCG AAAATTGGAT TCTACTTTGA CAAGTACTAC CAGTTTACCA 
CCATCATTAG CTTTACCTCC GCCACCGTCT TATGATAGAT CTTCCACACC AGCACAACTC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552019 ( XM_006559391.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006559454.1 Apis mellifera rootletin (LOC552019), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHd16378
Clone ID Related Accession (Same CDS sequence)	XM_006559391.3 , XM_006559391.2
Accession Version	XM_006559391.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	rootletin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007085.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006559391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAG AAAGAGGCAT AGGCAGTAAA ATGGATTCTA CAGGAAGTAC ACTAAGTGGA 
ACTGGAGATT GTTTAAGTGA AGAAGATCTT TCACCAGATG CATTGGTTAG ACAAAACTAT 
GAATTACGTC ATCGTCTTGA AGAAGAAGCA GCCAATTATA AAAGACGTTT GGATACATAT 
AGACAAGCAC AACAACATCA GGCTGCTCTT GTTTCACGTT TACAAGCCAA AGTATTGCAA 
TATAAACAAA AATGTTCAGA GTTAGAAAAT CAAATGGCAG AATCTATAGT ATATGATTCT 
AATAAAGTTG TAAGTTCTGC ACCTCCAACA ACTTCTGTTT TAGATGCAGC TCATCAAACA 
CTAAGAGAAA TACGTGAAGA ACAAATATAT GATTTAGATA CTGCTCTAAA AAAACTAAGT 
GAAGAACGTA AAAGATGTGA AAAATTGCTG CAATTAAATA CAACATTGAA AGATCAGTTA 
GAAGAATCTC ATCAAACAAA TGAAGCACTT ACAAATGATC TACAAAAATT AAGTAATGAT 
TGGGATATTT TAAGAGAAGA ATTAGCTATC AAAGAAGAAG AGTGGAGAGA AGAAGAGCAA 
GCTTTTAATG ATTATTATAC TTCAGAACAT AACAGATTAT TAATTCTTTG GCGCGATGTT 
GTTTCTGTAA AACGATTATT TGCAGATATA AAATCTAGTA CAGAAAGGGA TCTATTAAAA 
ATGAAAAATA ACATTATTTC TACTTTTAAT GATGTTTCAT CAGCTTGTAA TAATACAGGA 
TTTGCTATGA GAATGCAAGC AGCTATGCAT CCAATGATAT CTCAACAAGT TCAACAGGCA 
CAAGAACAGG TAACAACTGA TTTAAAAACA GAATTAATAA CACTTAAACA ACAGTATGAT 
GTAGCTCAAA ATGAAATTCG TATAAAGGAA GAAAAAATCA ATCAACTTAT TCGTGATGTT 
CACAGTTTGG AAGAAAGATG TGGAGAAGCA GAAACAGAAA TTCATCGCAA TACACGTTTG 
CAAGATGATA TAGAAATTTT ACAATCTGCA TTGAGAGATA TAGCACATGC TGTAATTCAA 
GATGCAGAAA GTCGCGAAAT TGAATCAACT CAAGCACCTC CTCATCTTCA TTTATCACCT 
ACTGGACCAA TTCCTCAAAA ATCACCAAAA AGAGGTACAA GAAGCAACAC CATTCCAGCT 
TTTGCTGAAA GTACTATAAG TGCTGTACAA GCAGCTCTCC ATAAATATCA ATTGACTATA 
CATGAACTTC AGGTGAAATT GCAAACTAAT AAAGAACAAC TACAAAATAC TCGCAAACAA 
TGCGAAAATG CGGAAGAGAA TGTTAAAACT CTAGAGAAAA AAGCAGAAGA ACTGATTATT 
GAATTAGATG CAGTTCGATT GCACTGTTCA CAACTTAATC AAGAAAAAGA TATGTTACAA 
AAAGGACTTG ATACTATAAG AATAGAAAAA AATACTCTTG AAAAAACCAG GATAGAACTT 
AATAATATGA TGGAGAATTT AAACAATGAT TGCGAAAAAT TGCAAAAAGC TAACAACAAA 
TTGCAAAAAA TTTGCGACAA TTTAGAAGAT GAAAAATTAT ATCTTCAAAG TGAACTCAAT 
AGATTATCTA AAGATGTAGA ATTGAGGGAA TTAAATTTGC GTTCAGAAGA AGATAGGTGC 
AGTAAAATGA GAGAGGAATT ATTAACTCTA CGTGAAGAAT TGAATAAAAC ATATCTCAGT 
AAAGATATGT TAGAACAACA AAAGTTAGAA ACAGATGGAT TAATTTCACA AATTGAAAAA 
AGCAAAGGTG ATTTAGAATT AGAATTGGAA CGCGTATTAT TAGAAAAATC AGACGTGCAA 
GAAGTTTTAA TGAAACTAGA AACAGTTTGC AGCAATCATG AACAAGAGAA ACAACGATTA 
CAAGAAGAAT TAAAAAAGGT AACAGAAGAA AAAAATAAGT TAGCAAATCA ATGTACGGAT 
CAACAAGGTG ATCTGGGTTC CTTAAGAAAA GAATTACTTC AAGCAGAACA AACTAGACTT 
GATTTAGAAT CAGAAAAAGT AACTTTGAAT GAAAAAGTGA AATTTCTGGA GATCGAAAAG 
GAGAAGATTG AAATAGAATT GGCACAAGTT ACGCGCGAAC GTGGAGATTT CAATAATCAA 
TTATCAGTTT TAGCACGAAA GAAAGAAACA TTAAACGAAG AATTGATGAG ACTCAAACAA 
AGATTAGAAC AGGCAAACGA AATGAATGGA AGAATAAATC GGAATTTAGA AGATCTTGTA 
AAAGATAATG AAGAAAAACA AGTTCTTTTA GAAACTAATG AAAAGGAAGT ACAACGATTA 
CAAGAGCAAC TTGCTTCAAT GCGTAGTGAG AAAGAGACAT TAGAAGGTGT TTTATTTGAT 
ACACAAACTA GTCTAGAAAA TATGCATGTG AAAAAGACTC AATTAGAAAA GGAACAGAAA 
GAACTTTTAA TAAAACAAGA AAGCTTAAAA GGACAAGTAG AAAGATTAAT GAAAGATTTG 
GAGAATAGCG AAAAACGAAT TCATGAAGTA AAGCAAACTT TAACGCAACA AAGTGGCGAT 
CAAGAAGCCG AATTTCAACA AATTATTTCT AACGTGAAAA AACATAGTGA AGAAAACATA 
AAAAAATTAA ATGAAGAAAA AGAACAAATA AAGATAAATT TAGAAAAACG ACTTCAGCAA 
TCTCTCTTGC AAGTTACTGG AGAAAAAGAT AATGAAATAA ATCAATTGCA GCAAAGAATA 
GATGAAATGC AACAACATAT AGAAAATTTG TGTCAGCAAC ATGAAGAAGT TCTTCTTAGA 
GCAGAAAATG ATAAACAGCA AGCTCTTCTT ATAGCTCATC ATGATCAGCA AGCATTAATG 
GAAAAACTTG AAACTGTTTT GCATGAAATG GAAGAAGAGA AAAATAATGT AGAACGAGTC 
AAACGAGAAG CTGCAGTAAA AACTGAACAA GAGCGTAATA ATATAAATCA ATTACGTGAT 
GAATTAAATC GTCTTAAAAC AAAATTAGAT GAAACTAGAT TAAAAACTGA TGAAGAAAAA 
ATAAAACTTG ATCTAAAAAT AGAAGAACTC TGGAAAGAAC GTGAATTAGC ACAAAGAGAA 
TCCGAAGAAT TACAAGTTCA ATTACATATG ACAGAAGATA AAGTTGATAG TTTACAAAAT 
CAATTGCATG AAACTATTAG AAAACTTAAA GATGCTGAAA ATCTTAACGA AACATTACGC 
AAAGAATTAG TAGATATTAG AAGACAATTA GGTGATTCCA CATATGAAAA AGAAAAGTAT 
AATAGTAGTA ATAAAGAATT ACGCGAGCAT GTAAAACGCA TTGAAAGCGA AAAAAGAGAA 
CAAAATAGAA TATTAGAAGA ATCATATCAA AAAATTTCAG CACTGGAAGA TATGAAAGTA 
AATGTAGATG CAGAGAGATC TCGTCTTCAA GCACAAATTC GTGATATGGA GAAAGAAATG 
TTGCAATTAC AAAAACAACT TCATTTTACT CAAGATGAAT TACAAAAATC ACATCAAAGT 
AATGCTCAAG CACAAAATGA TGAAAAAGAA TTGCAAGCTC GACTAACTAA CGAAACAGAA 
GAAAGAGAAC GTTTACAATT GCAGTTACAT CAAGTAAAAA AACAGTTAAT GGATGTTGAC 
AATAGTCTAA AAGTAACAAG ACAAGAACTT GGAAGATTGC GGTCACGTGC AGATGAAGAG 
AATGAACGGT GGAGAGTTAG AGAACAAGAA CTAGTAGTTC GTCTTGAGGA TAGTCGATGT 
CGTGAAAGAA AACTCGAGGA TCAAAAACAT AACTTAGAAG TTTGTTTAGC TGATGCTTCT 
CAACAAATTC AAGAACTGAA GGCACGTCTT GGAGGTTCCG AAGGACGAGT TCGAGCTTTA 
GATGCGCAAT TATCGCAATT AGAAATAACA AAAAAAGAAG TAGAACAAAA ATTAAGTAGC 
GTGGGATCGA CTTTACGTCG AATTGCAGGT ATTCAAATGG ATGGAAGTGT TAATATACCC 
TTTAAATTAT TGAGTCCATC AAGAAGGTGG AGTCCAGCAA GAGCTCAAGA TCATATTGAT 
TCTAGTAGAG ATGTGATTCT TGATGTTGAT CCTGAGATTA TTAGAAAAGG TGTACGATCT 
CTTATGCAAC AAGTAGCACA AATCGAACGT GAAAGAGATG ATTATAAAAC TGAATTATGT 
AATTTGAAAA AACAATTGGA TGAAAATCAA GAGATTCAAA ATAGATCTGA TATACAAATA 
AACACTTTAT TAACAAATAT AAGAATGCTT CAGGATGAAA AGAATTCATT GGAGGTAAAA 
CTTTCTCAAA AACAAAGTGG TTATGAAATG CAGTTAAATG CTTTACAACT AAAGTCAGAA 
GAATGCGAGC AATTACGCGA GAAGATTATC AATCTTGAAT TAATGATTAG TAATAATTCT 
GAAGAAAAAA TACAATTTGA GGAAAAACTA GATAAATTAA AACAAGTTTT AAACAAAGTA 
GAAAATGAAA AACGTAATTT ACAAGAAGAA CTTAGTAAAA GCGAATCCCG CGCTACGAAA 
TTAGAATTAC AAAGAATGTC ATTAGAAGGT GACCTTCAAA GATTGCAAAT GATGTTCCAA 
GAAAAAGATG CAAATATTCA TAAATTACAA GAACGAAATG ATACACAAAA TCGAACAATG 
ACAACTTTAG AAGAACGTTG TACTTCATTG AAATCTATGG TGGAACAATT AAATCTTGCA 
TTGGAAAAAG CATCTACTAC AGAAAATGAA TTAAAGAATG AAATTAATTC AATGCAACAT 
AATATTATGG AATTAACAAC TACTTTGCAA ACTTCTAATG AAAAAAATAA ACAGTTACAA 
AAACAAATTT CAAATGCTGA AAATGAACGT AGAATCTTAT CTGAACGTAT AGAATCAATG 
CAACAATCTT TAAATGATCT CAAACATACA AATCAAACAT TAACAGATCA AATTACACGT 
CTTCAAAATG AATTGGCAAA TAATGAAGTA CAACGTTGTG CCTTAGAATC TCAATTAAGA 
ATAGTTGCCT ATCCAACACA AGAAGAAAAT ATAAATAAGG ATGAAGAATT ATTACGACAG 
TTACAAATAG CACAAAGAGA GAGAAGTGAA ATGAGAGGAA AAATGGAAGC ACTTAATGAT 
AAAATGAAAT TATTGGAAGC TGATAAACGT AATTTAGAAC GACAACTATC GTTATTTAAA 
TCAACTAATC GTAGCAAAAG TTATGAACGT TATGAGAAAG CACATACAGA ATTATTAGGC 
ACAAGTTTCG ATATAGATCA TTATGAACAA GAAAATAGAG AATTAAGATT GAAAGTTCGT 
AGATTAGAAA CACAACTTGC AGAGAAGGAA GCTGAACTTA TAAGATTGAA ATCAAGTTAC 
ACTCATACAC ATTCCGTATT TGATTTTAAT CGAGATAGAA CGGGCGAAAT TGAAAGACTT 
AGAGCAGCTC AATTACAAGC TGAAAAATTG TTAGAAGCAA GAGAACAAAG TCATCGTCAA 
CAAGTTTTGC GTTTGGAAAA TCAGATACAA TTGTTACGCG AGCAACTTAA TCAAGAAATA 
AAACGTCGAC AATTATATGT TTTACGAAGT TCAAGGGCAG GTAGAGAAAT GCAACAATTA 
AGACAAGCCT TAGGCGATTC TTTAAGAACT GTAGCTCAAG ATCCATCATT AGACGCAGTA 
TTATTAGAAC ATGAAGCTCG AAAATTGGAT TCTACTTTGA CAAGTACTAC CAGTTTACCA 
CCATCATTAG CTTTACCTCC GCCACCGTCT TATGATAGAT CTTCCACACC AGCACAACTC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006559454.1
CDS	29..5854
Translation	MKIERGIGSKMDSTGSTLSGTGDCLSEEDLSPDALVRQNYELRHRLEEEAANYKRRLDTYRQAQQHQAALVSRLQAKVLQYKQKCSELENQMAESIVYDSNKVVSSAPPTTSVLDAAHQTLREIREEQIYDLDTALKKLSEERKRCEKLLQLNTTLKDQLEESHQTNEALTNDLQKLSNDWDILREELAIKEEEWREEEQAFNDYYTSEHNRLLILWRDVVSVKRLFADIKSSTERDLLKMKNNIISTFNDVSSACNNTGFAMRMQAAMHPMISQQVQQAQEQVTTDLKTELITLKQQYDVAQNEIRIKEEKINQLIRDVHSLEERCGEAETEIHRNTRLQDDIEILQSALRDIAHAVIQDAESREIESTQAPPHLHLSPTGPIPQKSPKRGTRSNTIPAFAESTISAVQAALHKYQLTIHELQVKLQTNKEQLQNTRKQCENAEENVKTLEKKAEELIIELDAVRLHCSQLNQEKDMLQKGLDTIRIEKNTLEKTRIELNNMMENLNNDCEKLQKANNKLQKICDNLEDEKLYLQSELNRLSKDVELRELNLRSEEDRCSKMREELLTLREELNKTYLSKDMLEQQKLETDGLISQIEKSKGDLELELERVLLEKSDVQEVLMKLETVCSNHEQEKQRLQEELKKVTEEKNKLANQCTDQQGDLGSLRKELLQAEQTRLDLESEKVTLNEKVKFLEIEKEKIEIELAQVTRERGDFNNQLSVLARKKETLNEELMRLKQRLEQANEMNGRINRNLEDLVKDNEEKQVLLETNEKEVQRLQEQLASMRSEKETLEGVLFDTQTSLENMHVKKTQLEKEQKELLIKQESLKGQVERLMKDLENSEKRIHEVKQTLTQQSGDQEAEFQQIISNVKKHSEENIKKLNEEKEQIKINLEKRLQQSLLQVTGEKDNEINQLQQRIDEMQQHIENLCQQHEEVLLRAENDKQQALLIAHHDQQALMEKLETVLHEMEEEKNNVERVKREAAVKTEQERNNINQLRDELNRLKTKLDETRLKTDEEKIKLDLKIEELWKERELAQRESEELQVQLHMTEDKVDSLQNQLHETIRKLKDAENLNETLRKELVDIRRQLGDSTYEKEKYNSSNKELREHVKRIESEKREQNRILEESYQKISALEDMKVNVDAERSRLQAQIRDMEKEMLQLQKQLHFTQDELQKSHQSNAQAQNDEKELQARLTNETEERERLQLQLHQVKKQLMDVDNSLKVTRQELGRLRSRADEENERWRVREQELVVRLEDSRCRERKLEDQKHNLEVCLADASQQIQELKARLGGSEGRVRALDAQLSQLEITKKEVEQKLSSVGSTLRRIAGIQMDGSVNIPFKLLSPSRRWSPARAQDHIDSSRDVILDVDPEIIRKGVRSLMQQVAQIERERDDYKTELCNLKKQLDENQEIQNRSDIQINTLLTNIRMLQDEKNSLEVKLSQKQSGYEMQLNALQLKSEECEQLREKIINLELMISNNSEEKIQFEEKLDKLKQVLNKVENEKRNLQEELSKSESRATKLELQRMSLEGDLQRLQMMFQEKDANIHKLQERNDTQNRTMTTLEERCTSLKSMVEQLNLALEKASTTENELKNEINSMQHNIMELTTTLQTSNEKNKQLQKQISNAENERRILSERIESMQQSLNDLKHTNQTLTDQITRLQNELANNEVQRCALESQLRIVAYPTQEENINKDEELLRQLQIAQRERSEMRGKMEALNDKMKLLEADKRNLERQLSLFKSTNRSKSYERYEKAHTELLGTSFDIDHYEQENRELRLKVRRLETQLAEKEAELIRLKSSYTHTHSVFDFNRDRTGEIERLRAAQLQAEKLLEAREQSHRQQVLRLENQIQLLREQLNQEIKRRQLYVLRSSRAGREMQQLRQALGDSLRTVAQDPSLDAVLLEHEARKLDSTLTSTTSLPPSLALPPPPSYDRSSTPAQLK

Target ORF information:

RefSeq Version	XM_006559391.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera rootletin (LOC552019), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006559391.2

ORF Insert Sequence:

ATGAAAATAG AAAGAGGCAT AGGCAGTAAA ATGGATTCTA CAGGAAGTAC ACTAAGTGGA 
ACTGGAGATT GTTTAAGTGA AGAAGATCTT TCACCAGATG CATTGGTTAG ACAAAACTAT 
GAATTACGTC ATCGTCTTGA AGAAGAAGCA GCCAATTATA AAAGACGTTT GGATACATAT 
AGACAAGCAC AACAACATCA GGCTGCTCTT GTTTCACGTT TACAAGCCAA AGTATTGCAA 
TATAAACAAA AATGTTCAGA GTTAGAAAAT CAAATGGCAG AATCTATAGT ATATGATTCT 
AATAAAGTTG TAAGTTCTGC ACCTCCAACA ACTTCTGTTT TAGATGCAGC TCATCAAACA 
CTAAGAGAAA TACGTGAAGA ACAAATATAT GATTTAGATA CTGCTCTAAA AAAACTAAGT 
GAAGAACGTA AAAGATGTGA AAAATTGCTG CAATTAAATA CAACATTGAA AGATCAGTTA 
GAAGAATCTC ATCAAACAAA TGAAGCACTT ACAAATGATC TACAAAAATT AAGTAATGAT 
TGGGATATTT TAAGAGAAGA ATTAGCTATC AAAGAAGAAG AGTGGAGAGA AGAAGAGCAA 
GCTTTTAATG ATTATTATAC TTCAGAACAT AACAGATTAT TAATTCTTTG GCGCGATGTT 
GTTTCTGTAA AACGATTATT TGCAGATATA AAATCTAGTA CAGAAAGGGA TCTATTAAAA 
ATGAAAAATA ACATTATTTC TACTTTTAAT GATGTTTCAT CAGCTTGTAA TAATACAGGA 
TTTGCTATGA GAATGCAAGC AGCTATGCAT CCAATGATAT CTCAACAAGT TCAACAGGCA 
CAAGAACAGG TAACAACTGA TTTAAAAACA GAATTAATAA CACTTAAACA ACAGTATGAT 
GTAGCTCAAA ATGAAATTCG TATAAAGGAA GAAAAAATCA ATCAACTTAT TCGTGATGTT 
CACAGTTTGG AAGAAAGATG TGGAGAAGCA GAAACAGAAA TTCATCGCAA TACACGTTTG 
CAAGATGATA TAGAAATTTT ACAATCTGCA TTGAGAGATA TAGCACATGC TGTAATTCAA 
GATGCAGAAA GTCGCGAAAT TGAATCAACT CAAGCACCTC CTCATCTTCA TTTATCACCT 
ACTGGACCAA TTCCTCAAAA ATCACCAAAA AGAGGTACAA GAAGCAACAC CATTCCAGCT 
TTTGCTGAAA GTACTATAAG TGCTGTACAA GCAGCTCTCC ATAAATATCA ATTGACTATA 
CATGAACTTC AGGTGAAATT GCAAACTAAT AAAGAACAAC TACAAAATAC TCGCAAACAA 
TGCGAAAATG CGGAAGAGAA TGTTAAAACT CTAGAGAAAA AAGCAGAAGA ACTGATTATT 
GAATTAGATG CAGTTCGATT GCACTGTTCA CAACTTAATC AAGAAAAAGA TATGTTACAA 
AAAGGACTTG ATACTATAAG AATAGAAAAA AATACTCTTG AAAAAACCAG GATAGAACTT 
AATAATATGA TGGAGAATTT AAACAATGAT TGCGAAAAAT TGCAAAAAGC TAACAACAAA 
TTGCAAAAAA TTTGCGACAA TTTAGAAGAT GAAAAATTAT ATCTTCAAAG TGAACTCAAT 
AGATTATCTA AAGATGTAGA ATTGAGGGAA TTAAATTTGC GTTCAGAAGA AGATAGGTGC 
AGTAAAATGA GAGAGGAATT ATTAACTCTA CGTGAAGAAT TGAATAAAAC ATATCTCAGT 
AAAGATATGT TAGAACAACA AAAGTTAGAA ACAGATGGAT TAATTTCACA AATTGAAAAA 
AGCAAAGGTG ATTTAGAATT AGAATTGGAA CGCGTATTAT TAGAAAAATC AGACGTGCAA 
GAAGTTTTAA TGAAACTAGA AACAGTTTGC AGCAATCATG AACAAGAGAA ACAACGATTA 
CAAGAAGAAT TAAAAAAGGT AACAGAAGAA AAAAATAAGT TAGCAAATCA ATGTACGGAT 
CAACAAGGTG ATCTGGGTTC CTTAAGAAAA GAATTACTTC AAGCAGAACA AACTAGACTT 
GATTTAGAAT CAGAAAAAGT AACTTTGAAT GAAAAAGTGA AATTTCTGGA GATCGAAAAG 
GAGAAGATTG AAATAGAATT GGCACAAGTT ACGCGCGAAC GTGGAGATTT CAATAATCAA 
TTATCAGTTT TAGCACGAAA GAAAGAAACA TTAAACGAAG AATTGATGAG ACTCAAACAA 
AGATTAGAAC AGGCAAACGA AATGAATGGA AGAATAAATC GGAATTTAGA AGATCTTGTA 
AAAGATAATG AAGAAAAACA AGTTCTTTTA GAAACTAATG AAAAGGAAGT ACAACGATTA 
CAAGAGCAAC TTGCTTCAAT GCGTAGTGAG AAAGAGACAT TAGAAGGTGT TTTATTTGAT 
ACACAAACTA GTCTAGAAAA TATGCATGTG AAAAAGACTC AATTAGAAAA GGAACAGAAA 
GAACTTTTAA TAAAACAAGA AAGCTTAAAA GGACAAGTAG AAAGATTAAT GAAAGATTTG 
GAGAATAGCG AAAAACGAAT TCATGAAGTA AAGCAAACTT TAACGCAACA AAGTGGCGAT 
CAAGAAGCCG AATTTCAACA AATTATTTCT AACGTGAAAA AACATAGTGA AGAAAACATA 
AAAAAATTAA ATGAAGAAAA AGAACAAATA AAGATAAATT TAGAAAAACG ACTTCAGCAA 
TCTCTCTTGC AAGTTACTGG AGAAAAAGAT AATGAAATAA ATCAATTGCA GCAAAGAATA 
GATGAAATGC AACAACATAT AGAAAATTTG TGTCAGCAAC ATGAAGAAGT TCTTCTTAGA 
GCAGAAAATG ATAAACAGCA AGCTCTTCTT ATAGCTCATC ATGATCAGCA AGCATTAATG 
GAAAAACTTG AAACTGTTTT GCATGAAATG GAAGAAGAGA AAAATAATGT AGAACGAGTC 
AAACGAGAAG CTGCAGTAAA AACTGAACAA GAGCGTAATA ATATAAATCA ATTACGTGAT 
GAATTAAATC GTCTTAAAAC AAAATTAGAT GAAACTAGAT TAAAAACTGA TGAAGAAAAA 
ATAAAACTTG ATCTAAAAAT AGAAGAACTC TGGAAAGAAC GTGAATTAGC ACAAAGAGAA 
TCCGAAGAAT TACAAGTTCA ATTACATATG ACAGAAGATA AAGTTGATAG TTTACAAAAT 
CAATTGCATG AAACTATTAG AAAACTTAAA GATGCTGAAA ATCTTAACGA AACATTACGC 
AAAGAATTAG TAGATATTAG AAGACAATTA GGTGATTCCA CATATGAAAA AGAAAAGTAT 
AATAGTAGTA ATAAAGAATT ACGCGAGCAT GTAAAACGCA TTGAAAGCGA AAAAAGAGAA 
CAAAATAGAA TATTAGAAGA ATCATATCAA AAAATTTCAG CACTGGAAGA TATGAAAGTA 
AATGTAGATG CAGAGAGATC TCGTCTTCAA GCACAAATTC GTGATATGGA GAAAGAAATG 
TTGCAATTAC AAAAACAACT TCATTTTACT CAAGATGAAT TACAAAAATC ACATCAAAGT 
AATGCTCAAG CACAAAATGA TGAAAAAGAA TTGCAAGCTC GACTAACTAA CGAAACAGAA 
GAAAGAGAAC GTTTACAATT GCAGTTACAT CAAGTAAAAA AACAGTTAAT GGATGTTGAC 
AATAGTCTAA AAGTAACAAG ACAAGAACTT GGAAGATTGC GGTCACGTGC AGATGAAGAG 
AATGAACGGT GGAGAGTTAG AGAACAAGAA CTAGTAGTTC GTCTTGAGGA TAGTCGATGT 
CGTGAAAGAA AACTCGAGGA TCAAAAACAT AACTTAGAAG TTTGTTTAGC TGATGCTTCT 
CAACAAATTC AAGAACTGAA GGCACGTCTT GGAGGTTCCG AAGGACGAGT TCGAGCTTTA 
GATGCGCAAT TATCGCAATT AGAAATAACA AAAAAAGAAG TAGAACAAAA ATTAAGTAGC 
GTGGGATCGA CTTTACGTCG AATTGCAGGT ATTCAAATGG ATGGAAGTGT TAATATACCC 
TTTAAATTAT TGAGTCCATC AAGAAGGTGG AGTCCAGCAA GAGCTCAAGA TCATATTGAT 
TCTAGTAGAG ATGTGATTCT TGATGTTGAT CCTGAGATTA TTAGAAAAGG TGTACGATCT 
CTTATGCAAC AAGTAGCACA AATCGAACGT GAAAGAGATG ATTATAAAAC TGAATTATGT 
AATTTGAAAA AACAATTGGA TGAAAATCAA GAGATTCAAA ATAGATCTGA TATACAAATA 
AACACTTTAT TAACAAATAT AAGAATGCTT CAGGATGAAA AGAATTCATT GGAGGTAAAA 
CTTTCTCAAA AACAAAGTGG TTATGAAATG CAGTTAAATG CTTTACAACT AAAGTCAGAA 
GAATGCGAGC AATTACGCGA GAAGATTATC AATCTTGAAT TAATGATTAG TAATAATTCT 
GAAGAAAAAA TACAATTTGA GGAAAAACTA GATAAATTAA AACAAGTTTT AAACAAAGTA 
GAAAATGAAA AACGTAATTT ACAAGAAGAA CTTAGTAAAA GCGAATCCCG CGCTACGAAA 
TTAGAATTAC AAAGAATGTC ATTAGAAGGT GACCTTCAAA GATTGCAAAT GATGTTCCAA 
GAAAAAGATG CAAATATTCA TAAATTACAA GAACGAAATG ATACACAAAA TCGAACAATG 
ACAACTTTAG AAGAACGTTG TACTTCATTG AAATCTATGG TGGAACAATT AAATCTTGCA 
TTGGAAAAAG CATCTACTAC AGAAAATGAA TTAAAGAATG AAATTAATTC AATGCAACAT 
AATATTATGG AATTAACAAC TACTTTGCAA ACTTCTAATG AAAAAAATAA ACAGTTACAA 
AAACAAATTT CAAATGCTGA AAATGAACGT AGAATCTTAT CTGAACGTAT AGAATCAATG 
CAACAATCTT TAAATGATCT CAAACATACA AATCAAACAT TAACAGATCA AATTACACGT 
CTTCAAAATG AATTGGCAAA TAATGAAGTA CAACGTTGTG CCTTAGAATC TCAATTAAGA 
ATAGTTGCCT ATCCAACACA AGAAGAAAAT ATAAATAAGG ATGAAGAATT ATTACGACAG 
TTACAAATAG CACAAAGAGA GAGAAGTGAA ATGAGAGGAA AAATGGAAGC ACTTAATGAT 
AAAATGAAAT TATTGGAAGC TGATAAACGT AATTTAGAAC GACAACTATC GTTATTTAAA 
TCAACTAATC GTAGCAAAAG TTATGAACGT TATGAGAAAG CACATACAGA ATTATTAGGC 
ACAAGTTTCG ATATAGATCA TTATGAACAA GAAAATAGAG AATTAAGATT GAAAGTTCGT 
AGATTAGAAA CACAACTTGC AGAGAAGGAA GCTGAACTTA TAAGATTGAA ATCAAGTTAC 
ACTCATACAC ATTCCGTATT TGATTTTAAT CGAGATAGAA CGGGCGAAAT TGAAAGACTT 
AGAGCAGCTC AATTACAAGC TGAAAAATTG TTAGAAGCAA GAGAACAAAG TCATCGTCAA 
CAAGTTTTGC GTTTGGAAAA TCAGATACAA TTGTTACGCG AGCAACTTAA TCAAGAAATA 
AAACGTCGAC AATTATATGT TTTACGAAGT TCAAGGGCAG GTAGAGAAAT GCAACAATTA 
AGACAAGCCT TAGGCGATTC TTTAAGAACT GTAGCTCAAG ATCCATCATT AGACGCAGTA 
TTATTAGAAC ATGAAGCTCG AAAATTGGAT TCTACTTTGA CAAGTACTAC CAGTTTACCA 
CCATCATTAG CTTTACCTCC GCCACCGTCT TATGATAGAT CTTCCACACC AGCACAACTC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC552019 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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