P4HB cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	P4HB
Entrez Gene ID	281373
Full Name	prolyl 4-hydroxylase subunit beta
Synonyms	PDI
General protein information	Preferred Names prolyl 4-hydroxylase subunit beta Names protein disulfide-isomerase PDI (E.C.5.3.4.1) cellular thyroid hormone binding protein p55 procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide prolyl 4-hydroxylase beta polypeptide prolyl 4-hydroxylase, beta subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 19 Map Location: 19q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_174135.3	NP_776560.1	protein disulfide-isomerase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	P4HB	XP_540488.2
Mus musculus (house mouse)	P4hb	NP_035162.1
Rattus norvegicus (Norway rat)	P4hb	NP_037130.1
Gallus gallus (chicken)	P4HB	NP_001185639.1
Danio rerio (zebrafish)	p4hb	NP_998529.3
Eremothecium gossypii	AGOS_AFR718W	NP_986266.2
Arabidopsis thaliana (thale cress)	PDIL1-4	NP_851234.1
Xenopus tropicalis (tropical clawed frog)	p4hb	NP_001034820.1
Homo sapiens (human)	P4HB	NP_000909.2
Pan troglodytes (chimpanzee)	P4HB	NP_001233358.1
Drosophila melanogaster (fruit fly)	Pdi	NP_524079.1
Saccharomyces cerevisiae (baker's yeast)	PDI1	NP_009887.1
Neurospora crassa	NCU09223	XP_959167.1
Oryza sativa (rice)	Os02g0100100	NP_001045579.1
Caenorhabditis elegans (roundworm)	pdi-2	NP_508778.1
Arabidopsis thaliana (thale cress)	PDIL1-3	NP_191056.2
	LOC714702	XP_002800695.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012407	XP_551775.3
Bos taurus (cattle)	P4HB	NP_776560.1
Kluyveromyces lactis	KLLA0C01111g	XP_452244.1
Schizosaccharomyces pombe (fission yeast)	SPAC1F5.02	NP_592871.1
Magnaporthe oryzae (rice blast fungus)	MGG_05753	XP_003710672.1

Gene Ontology
Bibliography

Related articles in PubMed

[Interaction of rotavirus with protein disulfide isomerase in vitro and cell system].
Calder?n MN, Guerrero CA, Dom?nguez Y, Garz?n E, Barreto SM, Acosta O
Biomedica : revista del Instituto Nacional de Salud31(1)70-81(2011 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

Sequence of membrane-associated thyroid hormone binding protein from bovine liver: its identity with protein disulphide isomerase.
Yamauchi K, Yamamoto T, Hayashi H, Koya S, Takikawa H, Toyoshima K, Horiuchi R
Biochemical and biophysical research communications146(3)1485-92(1987 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rotavirus-protein disulfide isomerase interaction was demonstrated in vitro as well as inMA104 cells and intestinal villi from suckling mice.
Title: [Interaction of rotavirus with protein disulfide isomerase in vitro and cell system].

The following P4HB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P4HB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41411	NM_174135.3 Latest version!	Bos taurus prolyl 4-hydroxylase subunit beta (P4HB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P4HB ( NM_174135.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776560.1 Bos taurus prolyl 4-hydroxylase subunit beta (P4HB), mRNA.

Price & Availability↑

CloneID	OBa41411
Clone ID Related Accession (Same CDS sequence)	NM_174135.3
Accession Version	NM_174135.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	protein disulfide-isomerase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M17596.1. On Feb 12, 2005 this sequence version replaced NM_174135.2. ##Evidence-Data-START## CDS exon combination :: BC146271.1, BT030719.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCGCC GCGCTCTGCT CTGCCTGGCC CTGACCGCGC TATTCCGCGC GGGTGCCGGC 
GCCCCCGACG AGGAGGACCA CGTCCTGGTG CTCCATAAGG GCAACTTCGA CGAGGCGCTG 
GCGGCCCACA AGTACCTGCT GGTGGAGTTC TACGCCCCAT GGTGCGGCCA CTGCAAGGCT 
CTGGCCCCGG AGTATGCCAA AGCAGCTGGG AAGCTGAAGG CAGAAGGTTC TGAGATCAGA 
CTGGCCAAGG TGGATGCCAC TGAAGAGTCT GACCTGGCCC AGCAGTATGG TGTCCGAGGC 
TACCCCACCA TCAAGTTCTT CAAGAATGGA GACACAGCTT CCCCCAAAGA GTACACAGCT 
GGCCGAGAAG CGGATGATAT CGTGAACTGG CTGAAGAAGC GCACGGGCCC CGCTGCCAGC 
ACGCTGTCCG ACGGGGCTGC TGCAGAGGCC TTGGTGGAGT CCAGTGAGGT GGCCGTCATT 
GGCTTCTTCA AGGACATGGA GTCGGACTCC GCAAAGCAGT TCTTCTTGGC AGCAGAGGTC 
ATTGATGACA TCCCCTTCGG GATCACATCT AACAGCGATG TGTTCTCCAA ATACCAGCTG 
GACAAGGATG GGGTTGTCCT CTTTAAGAAG TTTGACGAAG GCCGGAACAA CTTTGAGGGG 
GAGGTCACCA AAGAAAAGCT TCTGGACTTC ATCAAGCACA ACCAGTTGCC CCTGGTCATT 
GAGTTCACCG AGCAGACAGC CCCGAAGATC TTCGGAGGGG AAATCAAGAC TCACATCCTG 
CTGTTCCTGC CGAAAAGCGT GTCTGACTAT GAGGGCAAGC TGAGCAACTT CAAAAAAGCG 
GCTGAGAGCT TCAAGGGCAA GATCCTGTTT ATCTTCATCG ACAGCGACCA CACTGACAAC 
CAGCGCATCC TGGAATTCTT CGGCCTAAAG AAAGAGGAGT GCCCGGCCGT GCGCCTCATC 
ACGCTGGAGG AGGAGATGAC CAAATATAAG CCAGAGTCAG ATGAGCTGAC GGCAGAGAAG 
ATCACCGAGT TCTGCCACCG CTTCCTGGAG GGCAAGATTA AGCCCCACCT GATGAGCCAG 
GAGCTGCCTG ACGACTGGGA CAAGCAGCCT GTCAAAGTGC TGGTTGGGAA GAACTTTGAA 
GAGGTTGCTT TTGATGAGAA AAAGAACGTC TTTGTAGAGT TCTATGCCCC GTGGTGCGGT 
CACTGCAAGC AGCTGGCCCC CATCTGGGAT AAGCTGGGAG AGACGTACAA GGACCACGAG 
AACATAGTCA TCGCCAAGAT GGACTCCACG GCCAACGAGG TGGAGGCGGT GAAAGTGCAC 
AGCTTCCCCA CGCTCAAGTT CTTCCCCGCC AGCGCCGACA GGACGGTCAT CGACTACAAT 
GGGGAGCGGA CACTGGATGG TTTTAAGAAG TTCCTGGAGA GTGGTGGCCA GGATGGGGCC 
GGAGATGATG ACGATCTAGA AGATCTTGAA GAAGCAGAAG AGCCTGATCT GGAGGAAGAT 
GATGATCAAA AAGCTGTGAA AGATGAACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776560.1
CDS	6..1538
Translation	MLRRALLCLALTALFRAGAGAPDEEDHVLVLHKGNFDEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAASTLSDGAAAEALVESSEVAVIGFFKDMESDSAKQFFLAAEVIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYEGKLSNFKKAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQELPDDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDLEEDDDQKAVKDEL

Target ORF information:

RefSeq Version	NM_174135.3
Organism	Bos taurus(cattle)
Definition	Bos taurus prolyl 4-hydroxylase subunit beta (P4HB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174135.3

ORF Insert Sequence:

ATGCTGCGCC GCGCTCTGCT CTGCCTGGCC CTGACCGCGC TATTCCGCGC GGGTGCCGGC 
GCCCCCGACG AGGAGGACCA CGTCCTGGTG CTCCATAAGG GCAACTTCGA CGAGGCGCTG 
GCGGCCCACA AGTACCTGCT GGTGGAGTTC TACGCCCCAT GGTGCGGCCA CTGCAAGGCT 
CTGGCCCCGG AGTATGCCAA AGCAGCTGGG AAGCTGAAGG CAGAAGGTTC TGAGATCAGA 
CTGGCCAAGG TGGATGCCAC TGAAGAGTCT GACCTGGCCC AGCAGTATGG TGTCCGAGGC 
TACCCCACCA TCAAGTTCTT CAAGAATGGA GACACAGCTT CCCCCAAAGA GTACACAGCT 
GGCCGAGAAG CGGATGATAT CGTGAACTGG CTGAAGAAGC GCACGGGCCC CGCTGCCAGC 
ACGCTGTCCG ACGGGGCTGC TGCAGAGGCC TTGGTGGAGT CCAGTGAGGT GGCCGTCATT 
GGCTTCTTCA AGGACATGGA GTCGGACTCC GCAAAGCAGT TCTTCTTGGC AGCAGAGGTC 
ATTGATGACA TCCCCTTCGG GATCACATCT AACAGCGATG TGTTCTCCAA ATACCAGCTG 
GACAAGGATG GGGTTGTCCT CTTTAAGAAG TTTGACGAAG GCCGGAACAA CTTTGAGGGG 
GAGGTCACCA AAGAAAAGCT TCTGGACTTC ATCAAGCACA ACCAGTTGCC CCTGGTCATT 
GAGTTCACCG AGCAGACAGC CCCGAAGATC TTCGGAGGGG AAATCAAGAC TCACATCCTG 
CTGTTCCTGC CGAAAAGCGT GTCTGACTAT GAGGGCAAGC TGAGCAACTT CAAAAAAGCG 
GCTGAGAGCT TCAAGGGCAA GATCCTGTTT ATCTTCATCG ACAGCGACCA CACTGACAAC 
CAGCGCATCC TGGAATTCTT CGGCCTAAAG AAAGAGGAGT GCCCGGCCGT GCGCCTCATC 
ACGCTGGAGG AGGAGATGAC CAAATATAAG CCAGAGTCAG ATGAGCTGAC GGCAGAGAAG 
ATCACCGAGT TCTGCCACCG CTTCCTGGAG GGCAAGATTA AGCCCCACCT GATGAGCCAG 
GAGCTGCCTG ACGACTGGGA CAAGCAGCCT GTCAAAGTGC TGGTTGGGAA GAACTTTGAA 
GAGGTTGCTT TTGATGAGAA AAAGAACGTC TTTGTAGAGT TCTATGCCCC GTGGTGCGGT 
CACTGCAAGC AGCTGGCCCC CATCTGGGAT AAGCTGGGAG AGACGTACAA GGACCACGAG 
AACATAGTCA TCGCCAAGAT GGACTCCACG GCCAACGAGG TGGAGGCGGT GAAAGTGCAC 
AGCTTCCCCA CGCTCAAGTT CTTCCCCGCC AGCGCCGACA GGACGGTCAT CGACTACAAT 
GGGGAGCGGA CACTGGATGG TTTTAAGAAG TTCCTGGAGA GTGGTGGCCA GGATGGGGCC 
GGAGATGATG ACGATCTAGA AGATCTTGAA GAAGCAGAAG AGCCTGATCT GGAGGAAGAT 
GATGATCAAA AAGCTGTGAA AGATGAACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Interaction of rotavirus with protein disulfide isomerase in vitro and cell system].
Biomedica : revista del Instituto Nacional de Salud31(1)70-81(2011 Mar)
Calder?n MN,Guerrero CA,Dom?nguez Y,Garz?n E,Barreto SM,Acosta O

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

Sequence of membrane-associated thyroid hormone binding protein from bovine liver: its identity with protein disulphide isomerase.
Biochemical and biophysical research communications146(3)1485-92(1987 Aug)
Yamauchi K,Yamamoto T,Hayashi H,Koya S,Takikawa H,Toyoshima K,Horiuchi R

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P4HB cDNA ORF clone, Bos taurus(cattle)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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