COL5A2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL5A2
Entrez Gene ID	538590
Full Name	collagen type V alpha 2 chain
General protein information	Preferred Names collagen type V alpha 2 chain Names collagen alpha-2(V) chain LOW QUALITY PROTEIN: collagen alpha-2(V) chain collagen, type V, alpha 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 2 Map Location: 2q14-q15

mRNA and Protein(s)

mRNA	Protein	Name
XM_015462240.1	XP_015317726.1	LOW QUALITY PROTEIN: collagen alpha-2(V) chain isoform X2
XM_024979774.1	XP_024835542.1	collagen alpha-2(V) chain
XM_010801836.2	XP_010800138.1	collagen alpha-2(V) chain isoform X1

Pathways from BioSystems
Gene Ontology

The following COL5A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227645	XM_024979774.1 Latest version!	Bos taurus collagen type V alpha 2 chain (COL5A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa53936	XM_010801836.2 Latest version!	Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89500	XM_015462240.1 Latest version!	Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL5A2 ( XM_024979774.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835542.1 Bos taurus collagen type V alpha 2 chain (COL5A2), mRNA.

Price & Availability↑

CloneID	OBa227645
Clone ID Related Accession (Same CDS sequence)	XM_024979774.1
Accession Version	XM_024979774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-2(V) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG 
ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT 
GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT 
GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA 
GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC 
GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT 
CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG 
AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA 
AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT 
GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT 
GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG 
GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG 
CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT 
ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT 
CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT 
GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT 
GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT 
CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA 
CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA 
GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA 
AGGGGTGCTC CTGGCAATCG TGGCTTTCCA GGCTCTGATG GTTTACCTGG ACCAAAGGGC 
GCTCAAGGAG AGAGGGGTCC TGTTGGCTCT TCAGGACCTA AAGGAGGTCA GGGGGATCCA 
GGACGTCCAG GTGAACCTGG GCTTCCAGGT GCTCGGGGTC TGACAGGAAA TCCTGGTGTC 
CAGGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCTCCAG GAGAAGACGG CCGGCCGGGT 
CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCTGGGAGTA TGGGTCTTCC AGGCCCCAAA 
GGTAGCAGTG GTGATCCTGG AAAACCTGGA GAAGCAGGAA ATGCTGGTGT TCCTGGGCAG 
AGGGGAGCTC CTGGAAAAGA TGGCGAAGTT GGTCCTTCTG GTCCTGTGGG GCCCCCGGGT 
CTAGCCGGGG AGAGAGGAGA ACAGGGTCCT CCAGGCCCCA CTGGCTTTCA GGGGCTTCCT 
GGTCCTCCAG GGCCTCCTGG GGAAGCTGGA AAGCCAGGTG ATCAGGGGGT TCCTGGAGAT 
CCTGGGGCAG TTGGCCCACT AGGACCTAGA GGAGAACGAG GAAATCCAGG GGAAAGAGGA 
GAACCAGGAA TAACTGGACT TCCTGGTGAG AAAGGAATGG CTGGAGGACA TGGTCCTGAT 
GGTCCAAAAG GCAGTCCAGG ACCACCTGGA ACCCCTGGAG ATACAGGCCC ACCAGGGCTT 
CAAGGTATGC CAGGAGAAAG GGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT 
GGCATAGGAG AAAAAGGTGC TGAAGGTACA GCTGGAAATG ATGGAGCAAG AGGTCTTCCG 
GGTCCCTTGG GCCCTCCAGG TCCTTCAGGC CCTACTGGAG AAAAGGGAGA ACCCGGCCCT 
CGAGGTTTAG TCGGCCCGCC TGGCTCCCGT GGCAATCCCG GCTCACGTGG TGAAAATGGC 
CCAACTGGAG CTGTGGGTTT TGCGGGACCC CAGGGGCCGG ATGGGCAGCC TGGAGTGAAA 
GGTGAACCTG GAGAGCCTGG GCAGAAGGGA GACGCTGGCT CTCCTGGACC ACAAGGACTG 
GCAGGATCCC CAGGGCCTCA TGGTCCTAAT GGTGTTCCCG GACTGAAAGG TGGTCGAGGA 
ACTCAGGGTC CACCTGGTGC TACAGGATTT CCTGGTTCTG CTGGCAGAGT TGGACCTCCA 
GGTCCTGCTG GAGCTCCAGG ACCTGCGGGG CCCTTAGGGG AGCCCGGGAA GGAAGGACCT 
CCAGGTCTTC GTGGGGACCC CGGGTCTCAT GGACGAGTAG GAGATCGAGG ACCAGCTGGC 
CCTCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGACAACC TGGTCCAGAT 
GGTCCCCCTG GTCCAGCTGG AACTACTGGG CAAAGAGGAA TCGTCGGCAT GCCGGGGCAG 
CGTGGGGAGA GAGGGATGCC CGGCCTTCCA GGCCCAGCAG GCACACCAGG AAAAGTAGGA 
CCAACTGGTG CCCCAGGAGA TAAAGGTCCA CCTGGACCTG TTGGGCCCCC GGGCTCGAAT 
GGCCCTGTGG GGGAACCAGG ACCGGAGGGT CCAGCTGGTA ATGATGGTAC TCCAGGACGG 
GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC 
TCTCAGGGTG CCCCTGGAAC TCCTGGTCCT GTTGGTGCTC CGGGAGATGC AGGACAAAGA 
GGAGACCCGG GTTCTCGAGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGTTTA 
CTTGGACCGC AAGGACCTCG TGGTGACAAA GGCGATCATG GAGACAGAGG TGATCGAGGT 
CAGAAGGGTC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGACCTCC TGGTCCAACT 
GGTGAACAAG GCAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGACC CCCGGGTCCA 
GTTGGTCCTT CAGGCAAAGA AGGAAGCCCT GGGCCACTTG GGCCCATTGG GCCTCCTGGT 
GTGCGGGGCA GCGTTGGAGA AGCAGGCCCT GAGGGTCCTC CTGGTGAGCC TGGTCCCCCT 
GGCCCTCCGG GACCCCCTGG CCACCTTACA GCTGCTCTTG GGGACATCAT GGGGCACTAT 
GATGACAGCA TGCCAGACCC ACTTCCGGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC 
AAAAACAAAA CGGACCCGGG GGTACATGCG ACCCTGAAGT CACTCAGTAG TCAGATTGAA 
ACCATGCGCA GCCCTGATGG CTCTAGAAAG CACCCTGCCC GGACTTGTGA TGATTTAAAG 
CTTTGCCATT CTGCAAAGAA GAGCGGAGAG TACTGGATTG ACCCTAACCA GGGATCTGCT 
GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAT 
CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGCAAAT CTCCTGATAA TAAGCCTGTT 
TGGTATGGTC TTGATATGAA TCGAGGATCT CAGTTTGTTT ATGGAGACCA CCAGTCACCT 
AATGCAGCCA TTACTCAGAT GACCTTCTTG CGCCTTTTAT CGAAAGAAGC CTCCCAGAAC 
ATCACCTACA TCTGTAAAAA CAGTGTAGGA TACATGGATG ATCAAGCTAA GAACTTGAAG 
AAAGCTGTGG TTCTCAAAGG GTCAAATGAC TTAGAAATCA AAGCAGAGGG AAACGTTAGA 
TTCAGATACA TAGTTCTTCA TGATTCTTGC TCTAAACGAA ATGGAAACGT GGGCAAGACC 
ATCTTTGAAT ATAGAACGCA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCCCCTGTG 
GATGTTGGCA GTACAGACCA AGAATTTGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835542.1
CDS	417..4916
Translation	MMANWVEARPLLILTVLLGQFVSIKAQEEDEDEGYGEEIACTQNGQMYLNRDIWKPAPCQICVCDNGAILCDKIQCQDVLECADPVTPPGECCPVCPHTTGGGNTNFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGPRGERGPKGKPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGMSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGVGPAGPPGEPGDPGPMGPVGARGPEGPPGKPGEDGEPGRNGKPGEVGFAGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEVGATGSKGEAGPTGPMGAMGPMGPRGMPGERGRLGPQGAPGQRGAHGMPGKPGPMGPLGIPGSAGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSQGLPGAVGTDGTPGAKGPTGSPGTSGPPGLAGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPHGVQGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPVGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEAGKPGDQGVPGDPGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGSPGPPGTPGDTGPPGLQGMPGERGIAGTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPSGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGAPGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGLLGPQGPRGDKGDHGDRGDRGQKGHRGFTGLQGLPGPPGPTGEQGSAGIPGPFGPRGPPGPVGPSGKEGSPGPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDDSMPDPLPEFTEDQAAPDDKNKTDPGVHATLKSLSSQIETMRSPDGSRKHPARTCDDLKLCHSAKKSGEYWIDPNQGSAEDAIKVYCNMETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFVYGDHQSPNAAITQMTFLRLLSKEASQNITYICKNSVGYMDDQAKNLKKAVVLKGSNDLEIKAEGNVRFRYIVLHDSCSKRNGNVGKTIFEYRTQNVARLPIIDLAPVDVGSTDQEFGVEIGPVCFV

Target ORF information:

RefSeq Version	XM_024979774.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type V alpha 2 chain (COL5A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979774.1

ORF Insert Sequence:

ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG 
ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT 
GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT 
GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA 
GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC 
GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT 
CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG 
AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA 
AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT 
GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT 
GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG 
GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG 
CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT 
ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT 
CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT 
GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT 
GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT 
CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA 
CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA 
GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA 
AGGGGTGCTC CTGGCAATCG TGGCTTTCCA GGCTCTGATG GTTTACCTGG ACCAAAGGGC 
GCTCAAGGAG AGAGGGGTCC TGTTGGCTCT TCAGGACCTA AAGGAGGTCA GGGGGATCCA 
GGACGTCCAG GTGAACCTGG GCTTCCAGGT GCTCGGGGTC TGACAGGAAA TCCTGGTGTC 
CAGGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCTCCAG GAGAAGACGG CCGGCCGGGT 
CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCTGGGAGTA TGGGTCTTCC AGGCCCCAAA 
GGTAGCAGTG GTGATCCTGG AAAACCTGGA GAAGCAGGAA ATGCTGGTGT TCCTGGGCAG 
AGGGGAGCTC CTGGAAAAGA TGGCGAAGTT GGTCCTTCTG GTCCTGTGGG GCCCCCGGGT 
CTAGCCGGGG AGAGAGGAGA ACAGGGTCCT CCAGGCCCCA CTGGCTTTCA GGGGCTTCCT 
GGTCCTCCAG GGCCTCCTGG GGAAGCTGGA AAGCCAGGTG ATCAGGGGGT TCCTGGAGAT 
CCTGGGGCAG TTGGCCCACT AGGACCTAGA GGAGAACGAG GAAATCCAGG GGAAAGAGGA 
GAACCAGGAA TAACTGGACT TCCTGGTGAG AAAGGAATGG CTGGAGGACA TGGTCCTGAT 
GGTCCAAAAG GCAGTCCAGG ACCACCTGGA ACCCCTGGAG ATACAGGCCC ACCAGGGCTT 
CAAGGTATGC CAGGAGAAAG GGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT 
GGCATAGGAG AAAAAGGTGC TGAAGGTACA GCTGGAAATG ATGGAGCAAG AGGTCTTCCG 
GGTCCCTTGG GCCCTCCAGG TCCTTCAGGC CCTACTGGAG AAAAGGGAGA ACCCGGCCCT 
CGAGGTTTAG TCGGCCCGCC TGGCTCCCGT GGCAATCCCG GCTCACGTGG TGAAAATGGC 
CCAACTGGAG CTGTGGGTTT TGCGGGACCC CAGGGGCCGG ATGGGCAGCC TGGAGTGAAA 
GGTGAACCTG GAGAGCCTGG GCAGAAGGGA GACGCTGGCT CTCCTGGACC ACAAGGACTG 
GCAGGATCCC CAGGGCCTCA TGGTCCTAAT GGTGTTCCCG GACTGAAAGG TGGTCGAGGA 
ACTCAGGGTC CACCTGGTGC TACAGGATTT CCTGGTTCTG CTGGCAGAGT TGGACCTCCA 
GGTCCTGCTG GAGCTCCAGG ACCTGCGGGG CCCTTAGGGG AGCCCGGGAA GGAAGGACCT 
CCAGGTCTTC GTGGGGACCC CGGGTCTCAT GGACGAGTAG GAGATCGAGG ACCAGCTGGC 
CCTCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGACAACC TGGTCCAGAT 
GGTCCCCCTG GTCCAGCTGG AACTACTGGG CAAAGAGGAA TCGTCGGCAT GCCGGGGCAG 
CGTGGGGAGA GAGGGATGCC CGGCCTTCCA GGCCCAGCAG GCACACCAGG AAAAGTAGGA 
CCAACTGGTG CCCCAGGAGA TAAAGGTCCA CCTGGACCTG TTGGGCCCCC GGGCTCGAAT 
GGCCCTGTGG GGGAACCAGG ACCGGAGGGT CCAGCTGGTA ATGATGGTAC TCCAGGACGG 
GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC 
TCTCAGGGTG CCCCTGGAAC TCCTGGTCCT GTTGGTGCTC CGGGAGATGC AGGACAAAGA 
GGAGACCCGG GTTCTCGAGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGTTTA 
CTTGGACCGC AAGGACCTCG TGGTGACAAA GGCGATCATG GAGACAGAGG TGATCGAGGT 
CAGAAGGGTC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGACCTCC TGGTCCAACT 
GGTGAACAAG GCAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGACC CCCGGGTCCA 
GTTGGTCCTT CAGGCAAAGA AGGAAGCCCT GGGCCACTTG GGCCCATTGG GCCTCCTGGT 
GTGCGGGGCA GCGTTGGAGA AGCAGGCCCT GAGGGTCCTC CTGGTGAGCC TGGTCCCCCT 
GGCCCTCCGG GACCCCCTGG CCACCTTACA GCTGCTCTTG GGGACATCAT GGGGCACTAT 
GATGACAGCA TGCCAGACCC ACTTCCGGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC 
AAAAACAAAA CGGACCCGGG GGTACATGCG ACCCTGAAGT CACTCAGTAG TCAGATTGAA 
ACCATGCGCA GCCCTGATGG CTCTAGAAAG CACCCTGCCC GGACTTGTGA TGATTTAAAG 
CTTTGCCATT CTGCAAAGAA GAGCGGAGAG TACTGGATTG ACCCTAACCA GGGATCTGCT 
GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAT 
CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGCAAAT CTCCTGATAA TAAGCCTGTT 
TGGTATGGTC TTGATATGAA TCGAGGATCT CAGTTTGTTT ATGGAGACCA CCAGTCACCT 
AATGCAGCCA TTACTCAGAT GACCTTCTTG CGCCTTTTAT CGAAAGAAGC CTCCCAGAAC 
ATCACCTACA TCTGTAAAAA CAGTGTAGGA TACATGGATG ATCAAGCTAA GAACTTGAAG 
AAAGCTGTGG TTCTCAAAGG GTCAAATGAC TTAGAAATCA AAGCAGAGGG AAACGTTAGA 
TTCAGATACA TAGTTCTTCA TGATTCTTGC TCTAAACGAA ATGGAAACGT GGGCAAGACC 
ATCTTTGAAT ATAGAACGCA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCCCCTGTG 
GATGTTGGCA GTACAGACCA AGAATTTGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_010801836.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800138.1 Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa53936
Clone ID Related Accession (Same CDS sequence)	XM_010801836.2
Accession Version	XM_010801836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-2(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103830.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG 
ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT 
GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT 
GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA 
GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC 
GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT 
CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG 
AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA 
AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT 
GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT 
GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG 
GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG 
CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT 
ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT 
CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT 
GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT 
GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT 
CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA 
CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA 
GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA 
AGGGGTATTC CTTTTTTTCC TGACGTGTTC TACTTTGCTT TGAAACAGGG CGCTCAAGGA 
GAGAGGGGTC CTGTTGGCTC TTCAGGACCT AAAGGAGGTC AGGGGGATCC AGGACGTCCA 
GGTGAACCTG GGCTTCCAGG TGCTCGGGGT CTGACAGGAA ATCCTGGTGT CCAGGGTCCT 
GAAGGAAAAC TTGGACCTTT GGGTGCTCCA GGAGAAGACG GCCGGCCGGG TCCTCCAGGC 
TCCATAGGAA TCAGAGGGCA GCCTGGGAGT ATGGGTCTTC CAGGCCCCAA AGGTAGCAGT 
GGTGATCCTG GAAAACCTGG AGAAGCAGGA AATGCTGGTG TTCCTGGGCA GAGGGGAGCT 
CCTGGAAAAG ATGGCGAAGT TGGTCCTTCT GGTCCTGTGG GGCCCCCGGG TCTAGCCGGG 
GAGAGAGGAG AACAGGGTCC TCCAGGCCCC ACTGGCTTTC AGGGGCTTCC TGGTCCTCCA 
GGGCCTCCTG GGGAAGCTGG AAAGCCAGGT GATCAGGGGG TTCCTGGAGA TCCTGGGGCA 
GTTGGCCCAC TAGGACCTAG AGGAGAACGA GGAAATCCAG GGGAAAGAGG AGAACCAGGA 
ATAACTGGAC TTCCTGGTGA GAAAGGAATG GCTGGAGGAC ATGGTCCTGA TGGTCCAAAA 
GGCAGTCCAG GACCACCTGG AACCCCTGGA GATACAGGCC CACCAGGGCT TCAAGGTATG 
CCAGGAGAAA GGGGAATTGC AGGAACTCCT GGCCCCAAGG GTGACAGAGG TGGCATAGGA 
GAAAAAGGTG CTGAAGGTAC AGCTGGAAAT GATGGAGCAA GAGGTCTTCC GGGTCCCTTG 
GGCCCTCCAG GTCCTTCAGG CCCTACTGGA GAAAAGGGAG AACCCGGCCC TCGAGGTTTA 
GTCGGCCCGC CTGGCTCCCG TGGCAATCCC GGCTCACGTG GTGAAAATGG CCCAACTGGA 
GCTGTGGGTT TTGCGGGACC CCAGGGGCCG GATGGGCAGC CTGGAGTGAA AGGTGAACCT 
GGAGAGCCTG GGCAGAAGGG AGACGCTGGC TCTCCTGGAC CACAAGGACT GGCAGGATCC 
CCAGGGCCTC ATGGTCCTAA TGGTGTTCCC GGACTGAAAG GTGGTCGAGG AACTCAGGGT 
CCACCTGGTG CTACAGGATT TCCTGGTTCT GCTGGCAGAG TTGGACCTCC AGGTCCTGCT 
GGAGCTCCAG GACCTGCGGG GCCCTTAGGG GAGCCCGGGA AGGAAGGACC TCCAGGTCTT 
CGTGGGGACC CCGGGTCTCA TGGACGAGTA GGAGATCGAG GACCAGCTGG CCCTCCTGGT 
GGCCCAGGAG ACAAAGGGGA CCCAGGAGAA GATGGACAAC CTGGTCCAGA TGGTCCCCCT 
GGTCCAGCTG GAACTACTGG GCAAAGAGGA ATCGTCGGCA TGCCGGGGCA GCGTGGGGAG 
AGAGGGATGC CCGGCCTTCC AGGCCCAGCA GGCACACCAG GAAAAGTAGG ACCAACTGGT 
GCCCCAGGAG ATAAAGGTCC ACCTGGACCT GTTGGGCCCC CGGGCTCGAA TGGCCCTGTG 
GGGGAACCAG GACCGGAGGG TCCAGCTGGT AATGATGGTA CTCCAGGACG GGATGGTGCT 
GTTGGAGAAC GTGGTGATCG TGGAGACCCT GGGCCTGCAG GTCTGCCAGG CTCTCAGGGT 
GCCCCTGGAA CTCCTGGTCC TGTTGGTGCT CCGGGAGATG CAGGACAAAG AGGAGACCCG 
GGTTCTCGAG GTCCTATAGG ACCACCTGGT CGAGCTGGGA AACGTGGTTT ACTTGTAACA 
AGGGGTCCAA CTGGTGAACA AGGCAGTGCT GGAATCCCTG GACCATTTGG CCCAAGAGGA 
CCCCCGGGTC CAGTTGGTCC TTCAGGCAAA GAAGGAAGCC CTGGGCCACT TGGGCCCATT 
GGGCCTCCTG GTGTGCGGGG CAGCGTTGGA GAAGCAGGCC CTGAGGGTCC TCCTGGTGAG 
CCTGGTCCCC CTGGCCCTCC GGGACCCCCT GGCCACCTTA CAGCTGCTCT TGGGGACATC 
ATGGGGCACT ATGATGACAG CATGCCAGAC CCACTTCCGG AGTTTACTGA AGATCAGGCG 
GCTCCTGATG ACAAAAACAA AACGGACCCG GGGGTACATG CGACCCTGAA GTCACTCAGT 
AGTCAGATTG AAACCATGCG CAGCCCTGAT GGCTCTAGAA AGCACCCTGC CCGGACTTGT 
GATGATTTAA AGCTTTGCCA TTCTGCAAAG AAGAGCGGAG AGTACTGGAT TGACCCTAAC 
CAGGGATCTG CTGAAGATGC AATCAAAGTT TACTGCAACA TGGAAACAGG AGAAACATGT 
ATTTCAGCAA ATCCATCCAG TGTACCACGT AAAACCTGGT GGGCCAGCAA ATCTCCTGAT 
AATAAGCCTG TTTGGTATGG TCTTGATATG AATCGAGGAT CTCAGTTTGT TTATGGAGAC 
CACCAGTCAC CTAATGCAGC CATTACTCAG ATGACCTTCT TGCGCCTTTT ATCGAAAGAA 
GCCTCCCAGA ACATCACCTA CATCTGTAAA AACAGTGTAG GATACATGGA TGATCAAGCT 
AAGAACTTGA AGAAAGCTGT GGTTCTCAAA GGGTCAAATG ACTTAGAAAT CAAAGCAGAG 
GGAAACGTTA GATTCAGATA CATAGTTCTT CATGATTCTT GCTCTAAACG AAATGGAAAC 
GTGGGCAAGA CCATCTTTGA ATATAGAACG CAGAATGTGG CACGCTTGCC CATCATAGAT 
CTTGCCCCTG TGGATGTTGG CAGTACAGAC CAAGAATTTG GCGTTGAAAT TGGGCCAGTT 
TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800138.1
CDS	409..4800
Translation	MMANWVEARPLLILTVLLGQFVSIKAQEEDEDEGYGEEIACTQNGQMYLNRDIWKPAPCQICVCDNGAILCDKIQCQDVLECADPVTPPGECCPVCPHTTGGGNTNFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGPRGERGPKGKPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGMSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGVGPAGPPGEPGDPGPMGPVGARGPEGPPGKPGEDGEPGRNGKPGEVGFAGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEVGATGSKGEAGPTGPMGAMGPMGPRGMPGERGRLGPQGAPGQRGAHGMPGKPGPMGPLGIPGSAGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSQGLPGAVGTDGTPGAKGPTGSPGTSGPPGLAGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPHGVQGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGIPFFPDVFYFALKQGAQGERGPVGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEAGKPGDQGVPGDPGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGSPGPPGTPGDTGPPGLQGMPGERGIAGTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPSGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGAPGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGLLVTRGPTGEQGSAGIPGPFGPRGPPGPVGPSGKEGSPGPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDDSMPDPLPEFTEDQAAPDDKNKTDPGVHATLKSLSSQIETMRSPDGSRKHPARTCDDLKLCHSAKKSGEYWIDPNQGSAEDAIKVYCNMETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFVYGDHQSPNAAITQMTFLRLLSKEASQNITYICKNSVGYMDDQAKNLKKAVVLKGSNDLEIKAEGNVRFRYIVLHDSCSKRNGNVGKTIFEYRTQNVARLPIIDLAPVDVGSTDQEFGVEIGPVCFV

Target ORF information:

RefSeq Version	XM_010801836.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801836.2

ORF Insert Sequence:

ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG 
ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT 
GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT 
GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA 
GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC 
GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT 
CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG 
AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA 
AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT 
GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT 
GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG 
GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG 
CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT 
ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT 
CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT 
GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT 
GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT 
CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA 
CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA 
GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA 
AGGGGTATTC CTTTTTTTCC TGACGTGTTC TACTTTGCTT TGAAACAGGG CGCTCAAGGA 
GAGAGGGGTC CTGTTGGCTC TTCAGGACCT AAAGGAGGTC AGGGGGATCC AGGACGTCCA 
GGTGAACCTG GGCTTCCAGG TGCTCGGGGT CTGACAGGAA ATCCTGGTGT CCAGGGTCCT 
GAAGGAAAAC TTGGACCTTT GGGTGCTCCA GGAGAAGACG GCCGGCCGGG TCCTCCAGGC 
TCCATAGGAA TCAGAGGGCA GCCTGGGAGT ATGGGTCTTC CAGGCCCCAA AGGTAGCAGT 
GGTGATCCTG GAAAACCTGG AGAAGCAGGA AATGCTGGTG TTCCTGGGCA GAGGGGAGCT 
CCTGGAAAAG ATGGCGAAGT TGGTCCTTCT GGTCCTGTGG GGCCCCCGGG TCTAGCCGGG 
GAGAGAGGAG AACAGGGTCC TCCAGGCCCC ACTGGCTTTC AGGGGCTTCC TGGTCCTCCA 
GGGCCTCCTG GGGAAGCTGG AAAGCCAGGT GATCAGGGGG TTCCTGGAGA TCCTGGGGCA 
GTTGGCCCAC TAGGACCTAG AGGAGAACGA GGAAATCCAG GGGAAAGAGG AGAACCAGGA 
ATAACTGGAC TTCCTGGTGA GAAAGGAATG GCTGGAGGAC ATGGTCCTGA TGGTCCAAAA 
GGCAGTCCAG GACCACCTGG AACCCCTGGA GATACAGGCC CACCAGGGCT TCAAGGTATG 
CCAGGAGAAA GGGGAATTGC AGGAACTCCT GGCCCCAAGG GTGACAGAGG TGGCATAGGA 
GAAAAAGGTG CTGAAGGTAC AGCTGGAAAT GATGGAGCAA GAGGTCTTCC GGGTCCCTTG 
GGCCCTCCAG GTCCTTCAGG CCCTACTGGA GAAAAGGGAG AACCCGGCCC TCGAGGTTTA 
GTCGGCCCGC CTGGCTCCCG TGGCAATCCC GGCTCACGTG GTGAAAATGG CCCAACTGGA 
GCTGTGGGTT TTGCGGGACC CCAGGGGCCG GATGGGCAGC CTGGAGTGAA AGGTGAACCT 
GGAGAGCCTG GGCAGAAGGG AGACGCTGGC TCTCCTGGAC CACAAGGACT GGCAGGATCC 
CCAGGGCCTC ATGGTCCTAA TGGTGTTCCC GGACTGAAAG GTGGTCGAGG AACTCAGGGT 
CCACCTGGTG CTACAGGATT TCCTGGTTCT GCTGGCAGAG TTGGACCTCC AGGTCCTGCT 
GGAGCTCCAG GACCTGCGGG GCCCTTAGGG GAGCCCGGGA AGGAAGGACC TCCAGGTCTT 
CGTGGGGACC CCGGGTCTCA TGGACGAGTA GGAGATCGAG GACCAGCTGG CCCTCCTGGT 
GGCCCAGGAG ACAAAGGGGA CCCAGGAGAA GATGGACAAC CTGGTCCAGA TGGTCCCCCT 
GGTCCAGCTG GAACTACTGG GCAAAGAGGA ATCGTCGGCA TGCCGGGGCA GCGTGGGGAG 
AGAGGGATGC CCGGCCTTCC AGGCCCAGCA GGCACACCAG GAAAAGTAGG ACCAACTGGT 
GCCCCAGGAG ATAAAGGTCC ACCTGGACCT GTTGGGCCCC CGGGCTCGAA TGGCCCTGTG 
GGGGAACCAG GACCGGAGGG TCCAGCTGGT AATGATGGTA CTCCAGGACG GGATGGTGCT 
GTTGGAGAAC GTGGTGATCG TGGAGACCCT GGGCCTGCAG GTCTGCCAGG CTCTCAGGGT 
GCCCCTGGAA CTCCTGGTCC TGTTGGTGCT CCGGGAGATG CAGGACAAAG AGGAGACCCG 
GGTTCTCGAG GTCCTATAGG ACCACCTGGT CGAGCTGGGA AACGTGGTTT ACTTGTAACA 
AGGGGTCCAA CTGGTGAACA AGGCAGTGCT GGAATCCCTG GACCATTTGG CCCAAGAGGA 
CCCCCGGGTC CAGTTGGTCC TTCAGGCAAA GAAGGAAGCC CTGGGCCACT TGGGCCCATT 
GGGCCTCCTG GTGTGCGGGG CAGCGTTGGA GAAGCAGGCC CTGAGGGTCC TCCTGGTGAG 
CCTGGTCCCC CTGGCCCTCC GGGACCCCCT GGCCACCTTA CAGCTGCTCT TGGGGACATC 
ATGGGGCACT ATGATGACAG CATGCCAGAC CCACTTCCGG AGTTTACTGA AGATCAGGCG 
GCTCCTGATG ACAAAAACAA AACGGACCCG GGGGTACATG CGACCCTGAA GTCACTCAGT 
AGTCAGATTG AAACCATGCG CAGCCCTGAT GGCTCTAGAA AGCACCCTGC CCGGACTTGT 
GATGATTTAA AGCTTTGCCA TTCTGCAAAG AAGAGCGGAG AGTACTGGAT TGACCCTAAC 
CAGGGATCTG CTGAAGATGC AATCAAAGTT TACTGCAACA TGGAAACAGG AGAAACATGT 
ATTTCAGCAA ATCCATCCAG TGTACCACGT AAAACCTGGT GGGCCAGCAA ATCTCCTGAT 
AATAAGCCTG TTTGGTATGG TCTTGATATG AATCGAGGAT CTCAGTTTGT TTATGGAGAC 
CACCAGTCAC CTAATGCAGC CATTACTCAG ATGACCTTCT TGCGCCTTTT ATCGAAAGAA 
GCCTCCCAGA ACATCACCTA CATCTGTAAA AACAGTGTAG GATACATGGA TGATCAAGCT 
AAGAACTTGA AGAAAGCTGT GGTTCTCAAA GGGTCAAATG ACTTAGAAAT CAAAGCAGAG 
GGAAACGTTA GATTCAGATA CATAGTTCTT CATGATTCTT GCTCTAAACG AAATGGAAAC 
GTGGGCAAGA CCATCTTTGA ATATAGAACG CAGAATGTGG CACGCTTGCC CATCATAGAT 
CTTGCCCCTG TGGATGTTGG CAGTACAGAC CAAGAATTTG GCGTTGAAAT TGGGCCAGTT 
TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_015462240.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317726.1 Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X2, mRNA.

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CloneID	OBa89500
Clone ID Related Accession (Same CDS sequence)	XM_015462240.1
Accession Version	XM_015462240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: collagen alpha-2(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644570.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG 
ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT 
GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT 
GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA 
GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC 
GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT 
CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG 
AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA 
AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT 
GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT 
GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG 
GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG 
CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT 
ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT 
CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT 
GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT 
GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT 
CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA 
CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA 
GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA 
AGGGGGTGCT CCTGCAATCG TGGCTTTCCA GGCTCTGATG GTTTACCTGG ACACAAGGGC 
GCTCAAGGAG AGAGGGGTCC TGTTGGCTCT TCAGGACCTA AAGGAGGTCA GGGGGATCCA 
GGACGTCCAG GTGAACCTGG GCTTCCAGGT GCTCGGGGTC TGACAGGAAA TCCTGGTGTC 
CAGGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCTCCAG GAGAAGACGG CCGGCCGGGT 
CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCTGGGAGTA TGGGTCTTCC AGGCCCCAAA 
GGTAGCAGTG GTGATCCTGG AAAACCTGGA GAAGCAGGAA ATGCTGGTGT TCCTGGGCAG 
AGGGGAGCTC CTGGAAAAGA TGGCGAAGTT GGTCCTTCTG GTCCTGTGGG GCCCCCGGGT 
CTAGCCGGGG AGAGAGGAGA ACAGGGTCCT CCAGGCCCCA CTGGCTTTCA GGGGCTTCCT 
GGTCCTCCAG GGCCTCCTGG GGAAGCTGGA AAGCCAGGTG ATCAGGGGGT TCCTGGAGAT 
CCTGGGGCAG TTGGCCCACT AGGACCTAGA GGAGAACGAG GAAATCCAGG GGAAAGAGGA 
GAACCAGGAA TAACTGGACT TCCTGGTGAG AAAGGAATGG CTGGAGGACA TGGTCCTGAT 
GGTCCAAAAG GCAGTCCAGG ACCACCTGGA ACCCCTGGAG ATACAGGCCC ACCAGGGCTT 
CAAGGTATGC CAGGAGAAAG GGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT 
GGCATAGGAG AAAAAGGTGC TGAAGGTACA GCTGGAAATG ATGGAGCAAG AGGTCTTCCG 
GGTCCCTTGG GCCCTCCAGG TCCTTCAGGC CCTACTGGAG AAAAGGGAGA ACCCGGCCCT 
CGAGGTTTAG TCGGCCCGCC TGGCTCCCGT GGCAATCCCG GCTCACGTGG TGAAAATGGC 
CCAACTGGAG CTGTGGGTTT TGCGGGACCC CAGGGGCCGG ATGGGCAGCC TGGAGTGAAA 
GGTGAACCTG GAGAGCCTGG GCAGAAGGGA GACGCTGGCT CTCCTGGACC ACAAGGACTG 
GCAGGATCCC CAGGGCCTCA TGGTCCTAAT GGTGTTCCCG GACTGAAAGG TGGTCGAGGA 
ACTCAGGGTC CACCTGGTGC TACAGGATTT CCTGGTTCTG CTGGCAGAGT TGGACCTCCA 
GGTCCTGCTG GAGCTCCAGG ACCTGCGGGG CCCTTAGGGG AGCCCGGGAA GGAAGGACCT 
CCAGGTCTTC GTGGGGACCC CGGGTCTCAT GGACGAGTAG GAGATCGAGG ACCAGCTGGC 
CCTCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGACAACC TGGTCCAGAT 
GGTCCCCCTG GTCCAGCTGG AACTACTGGG CAAAGAGGAA TCGTCGGCAT GCCGGGGCAG 
CGTGGGGAGA GAGGGATGCC CGGCCTTCCA GGCCCAGCAG GCACACCAGG AAAAGTAGGA 
CCAACTGGTG CCCCAGGAGA TAAAGGTCCA CCTGGACCTG TTGGGCCCCC GGGCTCGAAT 
GGCCCTGTGG GGGAACCAGG ACCGGAGGGT CCAGCTGGTA ATGATGGTAC TCCAGGACGG 
GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC 
TCTCAGGGTG CCCCTGGAAC TCCTGGTCCT GTTGGTGCTC CGGGAGATGC AGGACAAAGA 
GGAGACCCGG GTTCTCGAGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGTTTA 
CTTGGACCGC AAGGACCTCG TGGTGACAAA GGCGATCATG GAGACAGAGG TGATCGAGGT 
CAGAAGGGTC ACAGAGGCTT TACTGGTNCT CAGGGTCTTC CTGGACCTCC GTAAGGTCCA 
ACTGGTGAAC AAGGCAGTGC TGGAATCCCT GGACCATTTG GCCCAAGAGG ACCCCCGGGT 
CCAGTTGGTC CTTCAGGCAA AGAAGGAAGC CCTGGGCCAC TTGGGCCCAT TGGGCCTCCT 
GGTGTGCGGG GCAGCGTTGG AGAAGCAGGC CCTGAGGGTC CTCCTGGTGA GCCTGGTCCC 
CCTGGCCCTC CGGGACCCCC TGGCCACCTT ACAGCTGCTC TTGGGGACAT CATGGGGCAC 
TATGATGACA GCATGCCAGA CCCACTTCCG GAGTTTACTG AAGATCAGGC GGCTCCTGAT 
GACAAAAACA AAACGGACCC GGGGGTACAT GCGACCCTGA AGTCACTCAG TAGTCAGATT 
GAAACCATGC GCAGCCCTGA TGGCTCTAGA AAGCACCCTG CCCGGACTTG TGATGATTTA 
AAGCTTTGCC ATTCTGCAAA GAAGAGCGGA GAGTACTGGA TTGACCCTAA CCAGGGATCT 
GCTGAAGATG CAATCAAAGT TTACTGCAAC ATGGAAACAG GAGAAACATG TATTTCAGCA 
AATCCATCCA GTGTACCACG TAAAACCTGG TGGGCCAGCA AATCTCCTGA TAATAAGCCT 
GTTTGGTATG GTCTTGATAT GAATCGAGGA TCTCAGTTTG TTTATGGAGA CCACCAGTCA 
CCTAATGCAG CCATTACTCA GATGACCTTC TTGCGCCTTT TATCGAAAGA AGCCTCCCAG 
AACATCACCT ACATCTGTAA AAACAGTGTA GGATACATGG ATGATCAAGC TAAGAACTTG 
AAGAAAGCTG TGGTTCTCAA AGGGTCAAAT GACTTAGAAA TCAAAGCAGA GGGAAACGTT 
AGATTCAGAT ACATAGTTCT TCATGATTCT TGCTCTAAAC GAAATGGAAA CGTGGGCAAG 
ACCATCTTTG AATATAGAAC GCAGAATGTG GCACGCTTGC CCATCATAGA TCTTGCCCCT 
GTGGATGTTG GCAGTACAGA CCAAGAATTT GGCGTTGAAA TTGGGCCAGT TTGTTTTGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317726.1
CDS	4..4506
Translation	MMANWVEARPLLILTVLLGQFVSIKAQEEDEDEGYGEEIACTQNGQMYLNRDIWKPAPCQICVCDNGAILCDKIQCQDVLECADPVTPPGECCPVCPHTTGGGNTNFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGPRGERGPKGKPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGMSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQGQQGGVGPAGPPGEPGDPGPMGPVGARGPEGPPGKPGEDGEPGRNGKPGEVGFAGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEVGATGSKGEAGPTGPMGAMGPMGPRGMPGERGRLGPQGAPGQRGAHGMPGKPGPMGPLGIPGSAGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSQGLPGAVGTDGTPGAKGPTGSPGTSGPPGLAGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPHGVQGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGCSCNRGFPGSDGLPGHKGAQGERGPVGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEAGKPGDQGVPGDPGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGSPGPPGTPGDTGPPGLQGMPGERGIAGTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPSGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGAPGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGLLGPQGPRGDKGDHGDRGDRGQKGHRGFTGnullQGLPGPP*GPTGEQGSAGIPGPFGPRGPPGPVGPSGKEGSPGPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDDSMPDPLPEFTEDQAAPDDKNKTDPGVHATLKSLSSQIETMRSPDGSRKHPARTCDDLKLCHSAKKSGEYWIDPNQGSAEDAIKVYCNMETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFVYGDHQSPNAAITQMTFLRLLSKEASQNITYICKNSVGYMDDQAKNLKKAVVLKGSNDLEIKAEGNVRFRYIVLHDSCSKRNGNVGKTIFEYRTQNVARLPIIDLAPVDVGSTDQEFGVEIGPVCFV

Target ORF information:

RefSeq Version	XM_015462240.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462240.1

ORF Insert Sequence:

ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC 
TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG 
ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT 
GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT 
GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA 
GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA 
GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC 
GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT 
CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG 
AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA 
AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT 
GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT 
GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT 
GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT 
CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG 
GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG 
CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT 
ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA 
GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT 
CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT 
GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT 
GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT 
CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA 
CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA 
GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA 
AGGGGGTGCT CCTGCAATCG TGGCTTTCCA GGCTCTGATG GTTTACCTGG ACACAAGGGC 
GCTCAAGGAG AGAGGGGTCC TGTTGGCTCT TCAGGACCTA AAGGAGGTCA GGGGGATCCA 
GGACGTCCAG GTGAACCTGG GCTTCCAGGT GCTCGGGGTC TGACAGGAAA TCCTGGTGTC 
CAGGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCTCCAG GAGAAGACGG CCGGCCGGGT 
CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCTGGGAGTA TGGGTCTTCC AGGCCCCAAA 
GGTAGCAGTG GTGATCCTGG AAAACCTGGA GAAGCAGGAA ATGCTGGTGT TCCTGGGCAG 
AGGGGAGCTC CTGGAAAAGA TGGCGAAGTT GGTCCTTCTG GTCCTGTGGG GCCCCCGGGT 
CTAGCCGGGG AGAGAGGAGA ACAGGGTCCT CCAGGCCCCA CTGGCTTTCA GGGGCTTCCT 
GGTCCTCCAG GGCCTCCTGG GGAAGCTGGA AAGCCAGGTG ATCAGGGGGT TCCTGGAGAT 
CCTGGGGCAG TTGGCCCACT AGGACCTAGA GGAGAACGAG GAAATCCAGG GGAAAGAGGA 
GAACCAGGAA TAACTGGACT TCCTGGTGAG AAAGGAATGG CTGGAGGACA TGGTCCTGAT 
GGTCCAAAAG GCAGTCCAGG ACCACCTGGA ACCCCTGGAG ATACAGGCCC ACCAGGGCTT 
CAAGGTATGC CAGGAGAAAG GGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT 
GGCATAGGAG AAAAAGGTGC TGAAGGTACA GCTGGAAATG ATGGAGCAAG AGGTCTTCCG 
GGTCCCTTGG GCCCTCCAGG TCCTTCAGGC CCTACTGGAG AAAAGGGAGA ACCCGGCCCT 
CGAGGTTTAG TCGGCCCGCC TGGCTCCCGT GGCAATCCCG GCTCACGTGG TGAAAATGGC 
CCAACTGGAG CTGTGGGTTT TGCGGGACCC CAGGGGCCGG ATGGGCAGCC TGGAGTGAAA 
GGTGAACCTG GAGAGCCTGG GCAGAAGGGA GACGCTGGCT CTCCTGGACC ACAAGGACTG 
GCAGGATCCC CAGGGCCTCA TGGTCCTAAT GGTGTTCCCG GACTGAAAGG TGGTCGAGGA 
ACTCAGGGTC CACCTGGTGC TACAGGATTT CCTGGTTCTG CTGGCAGAGT TGGACCTCCA 
GGTCCTGCTG GAGCTCCAGG ACCTGCGGGG CCCTTAGGGG AGCCCGGGAA GGAAGGACCT 
CCAGGTCTTC GTGGGGACCC CGGGTCTCAT GGACGAGTAG GAGATCGAGG ACCAGCTGGC 
CCTCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGACAACC TGGTCCAGAT 
GGTCCCCCTG GTCCAGCTGG AACTACTGGG CAAAGAGGAA TCGTCGGCAT GCCGGGGCAG 
CGTGGGGAGA GAGGGATGCC CGGCCTTCCA GGCCCAGCAG GCACACCAGG AAAAGTAGGA 
CCAACTGGTG CCCCAGGAGA TAAAGGTCCA CCTGGACCTG TTGGGCCCCC GGGCTCGAAT 
GGCCCTGTGG GGGAACCAGG ACCGGAGGGT CCAGCTGGTA ATGATGGTAC TCCAGGACGG 
GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC 
TCTCAGGGTG CCCCTGGAAC TCCTGGTCCT GTTGGTGCTC CGGGAGATGC AGGACAAAGA 
GGAGACCCGG GTTCTCGAGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGTTTA 
CTTGGACCGC AAGGACCTCG TGGTGACAAA GGCGATCATG GAGACAGAGG TGATCGAGGT 
CAGAAGGGTC ACAGAGGCTT TACTGGTNCT CAGGGTCTTC CTGGACCTCC GTAAGGTCCA 
ACTGGTGAAC AAGGCAGTGC TGGAATCCCT GGACCATTTG GCCCAAGAGG ACCCCCGGGT 
CCAGTTGGTC CTTCAGGCAA AGAAGGAAGC CCTGGGCCAC TTGGGCCCAT TGGGCCTCCT 
GGTGTGCGGG GCAGCGTTGG AGAAGCAGGC CCTGAGGGTC CTCCTGGTGA GCCTGGTCCC 
CCTGGCCCTC CGGGACCCCC TGGCCACCTT ACAGCTGCTC TTGGGGACAT CATGGGGCAC 
TATGATGACA GCATGCCAGA CCCACTTCCG GAGTTTACTG AAGATCAGGC GGCTCCTGAT 
GACAAAAACA AAACGGACCC GGGGGTACAT GCGACCCTGA AGTCACTCAG TAGTCAGATT 
GAAACCATGC GCAGCCCTGA TGGCTCTAGA AAGCACCCTG CCCGGACTTG TGATGATTTA 
AAGCTTTGCC ATTCTGCAAA GAAGAGCGGA GAGTACTGGA TTGACCCTAA CCAGGGATCT 
GCTGAAGATG CAATCAAAGT TTACTGCAAC ATGGAAACAG GAGAAACATG TATTTCAGCA 
AATCCATCCA GTGTACCACG TAAAACCTGG TGGGCCAGCA AATCTCCTGA TAATAAGCCT 
GTTTGGTATG GTCTTGATAT GAATCGAGGA TCTCAGTTTG TTTATGGAGA CCACCAGTCA 
CCTAATGCAG CCATTACTCA GATGACCTTC TTGCGCCTTT TATCGAAAGA AGCCTCCCAG 
AACATCACCT ACATCTGTAA AAACAGTGTA GGATACATGG ATGATCAAGC TAAGAACTTG 
AAGAAAGCTG TGGTTCTCAA AGGGTCAAAT GACTTAGAAA TCAAAGCAGA GGGAAACGTT 
AGATTCAGAT ACATAGTTCT TCATGATTCT TGCTCTAAAC GAAATGGAAA CGTGGGCAAG 
ACCATCTTTG AATATAGAAC GCAGAATGTG GCACGCTTGC CCATCATAGA TCTTGCCCCT 
GTGGATGTTG GCAGTACAGA CCAAGAATTT GGCGTTGAAA TTGGGCCAGT TTGTTTTGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL5A2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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