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DACT1 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol DACT1
Entrez Gene ID 538778
Full Name dishevelled binding antagonist of beta catenin 1
General protein information
Preferred Names

dishevelled binding antagonist of beta catenin 1

Names

dapper homolog 1
dapper, antagonist of beta-catenin, homolog 1
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_002690960.5 XP_002691006.1 dapper homolog 1 isoform X1
XM_015465163.1 XP_015320649.1 dapper homolog 1 isoform X2
XM_002690959.5 XP_002691005.1 dapper homolog 1 isoform X2
XM_015465162.1 XP_015320648.1 dapper homolog 1 isoform X1
XM_002690959.4 XP_002691005.1 dapper homolog 1 isoform X2
XM_002690960.4 XP_002691006.1 dapper homolog 1 isoform X1

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Dact1 NP_001177395.1
Danio rerio (zebrafish) dact1 NP_999896.2
Homo sapiens (human) DACT1 NP_057735.2
DACT1 XP_001092593.1
Xenopus tropicalis (tropical clawed frog) dact1 NP_001007950.1
Rattus norvegicus (Norway rat) Dact1 XP_576044.1
Gallus gallus (chicken) DACT1 NP_001038157.1
Pan troglodytes (chimpanzee) DACT1 XP_001165895.1
Canis lupus familiaris (dog) DACT1 XP_003435129.1
Bos taurus (cattle) DACT1 XP_002691006.1

Gene Ontology

The following DACT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DACT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa23429 XM_002690960.5
Latest version!		 Bos taurus dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa23429 XM_015465162.1
Latest version!		 Bos taurus dishevelled-binding antagonist of beta-catenin 1 (DACT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa23430 XM_002690959.5
Latest version!		 Bos taurus dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa23430 XM_015465163.1
Latest version!		 Bos taurus dishevelled-binding antagonist of beta-catenin 1 (DACT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
View Old Accession Versions >>
OBa23430 XM_002690959.4 Bos taurus dishevelled-binding antagonist of beta-catenin 1 (DACT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa23429 XM_002690960.4 Bos taurus dishevelled-binding antagonist of beta-catenin 1 (DACT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa23429
Clone ID Related Accession (Same CDS sequence) XM_002690960.5 , XM_015465162.1 , XM_002690960.4
Accession Version XM_002690960.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2526bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product dapper homolog 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690960.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATGGCT GGAATATAAA 
GAAGGCCATT GTGAAGACCA GGCCTCAAGG GCAGTTGACT GTTCCCCCTC CACACCACAA 
TCTAATTCCC TTGCTGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGCGA TCTGGTGTCT 
AAAAATGGGA ATGATGTGTA TCGCTACCCC AGTCCACTTC ATGCCGTGGC CGTGCAGAGC 
CCAATGTTCC TCCTTTGCCT GACTGGCAAC CCACTGAGGG AAGAGGAGCG GCTTGGTAAC 
CATGCCAATG ACGTTTGTGC GGTATCTGAG CTGGACGCCG TCAAGACAGA CACTAACTCA 
CCATCTCCAA GCAGTTTGTG GTCTGCTCCC CATCCTTCAT CCAGCAAGAA GATGGATGGT 
TACATTCTGA GCCTGGTCCA GAAAAAAACC CACCCAGTAA GGACCAACAA ACCGAGGACC 
AGTGTGAACG CTGACCCCAC GAAGGGGCTT CTGAGGAATG GGAGCGTTTG TGTCAGAGTG 
ACTGGTGGTG TCTCACAGGG CAACAATGGG AACCTTAAGA ATTCGAAACA GGTGCCTTTG 
CCCTCCGGAG GCATCCCTTC TTTGGACAGT GGGGGCTTCT CCCCACCAAA ACAGTGGTCA 
AAAGAATCAA AGCCAGAACC TCTGGAAAGC AAGAGGTTGC CCGCGCCTGA AGGCTGTTCT 
CCAGGCACTG CTACCGAACT TCAAGGTAAG CATTTGCCCA AAAATGCCAA GCCCGTCTCC 
CAGGAACATG CGAGGTGTCC CCCCGCTGGG ACAGGGGAGT CCTCTAAGGA AAGCGGGCAG 
ATCCCAGCTG CCTCTCCGAA AGAGAGCCCT GGGAGGGTCC TCGCACCGCT GCAAGAGAAC 
AAAGTGGTCC AGCCACTGAA AAAGGTGCCA CAGAAAAACA GCCCGCAGGC AGGCACTCTG 
GCGCCCCCTC CCGCCGCCTC AGCCCTGCTG CCCCCCACTT TCCCGGCGGA AGAGCGGCCG 
GCCCTGGATT TCAGAAGCGA GGGCTCTTCT TCTCAGAGCC TGGAGGAAGG GCCGCCGGGG 
AAGGCGCCGC CCGTCCTGGC GCCGCCTCCG GGGGTCAGGC CACCCCGGGG TGCGCGCCCC 
GCGGCCGCCC CGAGGGGCTC CGGCGTGAAG CACCGGGCGC TGGCCGTCCA CGGGCCGGAA 
AGTGCTCTGC CCCCCGTGAG GGAGAAGGCC CGGGTGGCCG GCAAGAAGTG TCGGTTCCCC 
GACGACACGG ATACAAATAA GAAACTGCGG CGAGCCCCGG CTAAGGCGCG GCGGGGCGGG 
GGCGGCCAGC CGGACGCGGG GCTCCCCAGC CGGCCTCTGG GGGCGGGCCA CCGCGCAGGG 
AGCCGGGCGC ACGGCCACGG CCACGGCCGG GAGGCGGTGG TGGCCAAGCC CAAGCACAAG 
CGCACCGACT CGCGGCGGTG GAGGTCGGCC GCCGAGGTGT CGTACGAGGA GGCGCTGCGG 
CGCGCGCGGC GGGGCCGACG GGAGCCCGCG GGGCTGTTTG CGGCCGGGCC TTACGCCAGC 
CCCTACGCGT ACGCGGCCAG CGACTCCGAG TACTCGGCCG AGTGCGAGTC CCTGTTCCAC 
TCCACTGTGC TGGACACCAG CGAGGACGAG CGCAGCAACT ACACCACCAA CTGCTTCGGC 
GACAGCGAGT CCAGCGTGAG CGAGGGCGAC TTCGTGGGCG ACAGCACGAG CACCAGCGAC 
TCGGAGGAGA GCGGGGGCTT GATATGGTCC CAGTTCGTGC AGACGCTCCC CCTGCAGCCG 
GTCCCGGCCT CCGACCTTCG CCACAACCCC ACCAAAACCT TCGTCAAGAT TAAGGCCTCG 
CACAACCTCA AGAAGAAGAT CCTCCGCTTT CGATCGGGCT CTTTGAAACT GATGACGACG 
GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002691006.1
CDS96..2621
Translation

Target ORF information:

RefSeq Version XM_002690960.5
Organism Bos taurus(cattle)
Definition Bos taurus dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002690960.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATGGCT GGAATATAAA 
GAAGGCCATT GTGAAGACCA GGCCTCAAGG GCAGTTGACT GTTCCCCCTC CACACCACAA 
TCTAATTCCC TTGCTGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGCGA TCTGGTGTCT 
AAAAATGGGA ATGATGTGTA TCGCTACCCC AGTCCACTTC ATGCCGTGGC CGTGCAGAGC 
CCAATGTTCC TCCTTTGCCT GACTGGCAAC CCACTGAGGG AAGAGGAGCG GCTTGGTAAC 
CATGCCAATG ACGTTTGTGC GGTATCTGAG CTGGACGCCG TCAAGACAGA CACTAACTCA 
CCATCTCCAA GCAGTTTGTG GTCTGCTCCC CATCCTTCAT CCAGCAAGAA GATGGATGGT 
TACATTCTGA GCCTGGTCCA GAAAAAAACC CACCCAGTAA GGACCAACAA ACCGAGGACC 
AGTGTGAACG CTGACCCCAC GAAGGGGCTT CTGAGGAATG GGAGCGTTTG TGTCAGAGTG 
ACTGGTGGTG TCTCACAGGG CAACAATGGG AACCTTAAGA ATTCGAAACA GGTGCCTTTG 
CCCTCCGGAG GCATCCCTTC TTTGGACAGT GGGGGCTTCT CCCCACCAAA ACAGTGGTCA 
AAAGAATCAA AGCCAGAACC TCTGGAAAGC AAGAGGTTGC CCGCGCCTGA AGGCTGTTCT 
CCAGGCACTG CTACCGAACT TCAAGGTAAG CATTTGCCCA AAAATGCCAA GCCCGTCTCC 
CAGGAACATG CGAGGTGTCC CCCCGCTGGG ACAGGGGAGT CCTCTAAGGA AAGCGGGCAG 
ATCCCAGCTG CCTCTCCGAA AGAGAGCCCT GGGAGGGTCC TCGCACCGCT GCAAGAGAAC 
AAAGTGGTCC AGCCACTGAA AAAGGTGCCA CAGAAAAACA GCCCGCAGGC AGGCACTCTG 
GCGCCCCCTC CCGCCGCCTC AGCCCTGCTG CCCCCCACTT TCCCGGCGGA AGAGCGGCCG 
GCCCTGGATT TCAGAAGCGA GGGCTCTTCT TCTCAGAGCC TGGAGGAAGG GCCGCCGGGG 
AAGGCGCCGC CCGTCCTGGC GCCGCCTCCG GGGGTCAGGC CACCCCGGGG TGCGCGCCCC 
GCGGCCGCCC CGAGGGGCTC CGGCGTGAAG CACCGGGCGC TGGCCGTCCA CGGGCCGGAA 
AGTGCTCTGC CCCCCGTGAG GGAGAAGGCC CGGGTGGCCG GCAAGAAGTG TCGGTTCCCC 
GACGACACGG ATACAAATAA GAAACTGCGG CGAGCCCCGG CTAAGGCGCG GCGGGGCGGG 
GGCGGCCAGC CGGACGCGGG GCTCCCCAGC CGGCCTCTGG GGGCGGGCCA CCGCGCAGGG 
AGCCGGGCGC ACGGCCACGG CCACGGCCGG GAGGCGGTGG TGGCCAAGCC CAAGCACAAG 
CGCACCGACT CGCGGCGGTG GAGGTCGGCC GCCGAGGTGT CGTACGAGGA GGCGCTGCGG 
CGCGCGCGGC GGGGCCGACG GGAGCCCGCG GGGCTGTTTG CGGCCGGGCC TTACGCCAGC 
CCCTACGCGT ACGCGGCCAG CGACTCCGAG TACTCGGCCG AGTGCGAGTC CCTGTTCCAC 
TCCACTGTGC TGGACACCAG CGAGGACGAG CGCAGCAACT ACACCACCAA CTGCTTCGGC 
GACAGCGAGT CCAGCGTGAG CGAGGGCGAC TTCGTGGGCG ACAGCACGAG CACCAGCGAC 
TCGGAGGAGA GCGGGGGCTT GATATGGTCC CAGTTCGTGC AGACGCTCCC CCTGCAGCCG 
GTCCCGGCCT CCGACCTTCG CCACAACCCC ACCAAAACCT TCGTCAAGAT TAAGGCCTCG 
CACAACCTCA AGAAGAAGAT CCTCCGCTTT CGATCGGGCT CTTTGAAACT GATGACGACG 
GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa23429
Clone ID Related Accession (Same CDS sequence) XM_002690960.5 , XM_015465162.1 , XM_002690960.4
Accession Version XM_015465162.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2526bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product dapper homolog 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATGGCT GGAATATAAA 
GAAGGCCATT GTGAAGACCA GGCCTCAAGG GCAGTTGACT GTTCCCCCTC CACACCACAA 
TCTAATTCCC TTGCTGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGCGA TCTGGTGTCT 
AAAAATGGGA ATGATGTGTA TCGCTACCCC AGTCCACTTC ATGCCGTGGC CGTGCAGAGC 
CCAATGTTCC TCCTTTGCCT GACTGGCAAC CCACTGAGGG AAGAGGAGCG GCTTGGTAAC 
CATGCCAATG ACGTTTGTGC GGTATCTGAG CTGGACGCCG TCAAGACAGA CACTAACTCA 
CCATCTCCAA GCAGTTTGTG GTCTGCTCCC CATCCTTCAT CCAGCAAGAA GATGGATGGT 
TACATTCTGA GCCTGGTCCA GAAAAAAACC CACCCAGTAA GGACCAACAA ACCGAGGACC 
AGTGTGAACG CTGACCCCAC GAAGGGGCTT CTGAGGAATG GGAGCGTTTG TGTCAGAGTG 
ACTGGTGGTG TCTCACAGGG CAACAATGGG AACCTTAAGA ATTCGAAACA GGTGCCTTTG 
CCCTCCGGAG GCATCCCTTC TTTGGACAGT GGGGGCTTCT CCCCACCAAA ACAGTGGTCA 
AAAGAATCAA AGCCAGAACC TCTGGAAAGC AAGAGGTTGC CCGCGCCTGA AGGCTGTTCT 
CCAGGCACTG CTACCGAACT TCAAGGTAAG CATTTGCCCA AAAATGCCAA GCCCGTCTCC 
CAGGAACATG CGAGGTGTCC CCCCGCTGGG ACAGGGGAGT CCTCTAAGGA AAGCGGGCAG 
ATCCCAGCTG CCTCTCCGAA AGAGAGCCCT GGGAGGGTCC TCGCACCGCT GCAAGAGAAC 
AAAGTGGTCC AGCCACTGAA AAAGGTGCCA CAGAAAAACA GCCCGCAGGC AGGCACTCTG 
GCGCCCCCTC CCGCCGCCTC AGCCCTGCTG CCCCCCACTT TCCCGGCGGA AGAGCGGCCG 
GCCCTGGATT TCAGAAGCGA GGGCTCTTCT TCTCAGAGCC TGGAGGAAGG GCCGCCGGGG 
AAGGCGCCGC CCGTCCTGGC GCCGCCTCCG GGGGTCAGGC CACCCCGGGG TGCGCGCCCC 
GCGGCCGCCC CGAGGGGCTC CGGCGTGAAG CACCGGGCGC TGGCCGTCCA CGGGCCGGAA 
AGTGCTCTGC CCCCCGTGAG GGAGAAGGCC CGGGTGGCCG GCAAGAAGTG TCGGTTCCCC 
GACGACACGG ATACAAATAA GAAACTGCGG CGAGCCCCGG CTAAGGCGCG GCGGGGCGGG 
GGCGGCCAGC CGGACGCGGG GCTCCCCAGC CGGCCTCTGG GGGCGGGCCA CCGCGCAGGG 
AGCCGGGCGC ACGGCCACGG CCACGGCCGG GAGGCGGTGG TGGCCAAGCC CAAGCACAAG 
CGCACCGACT CGCGGCGGTG GAGGTCGGCC GCCGAGGTGT CGTACGAGGA GGCGCTGCGG 
CGCGCGCGGC GGGGCCGACG GGAGCCCGCG GGGCTGTTTG CGGCCGGGCC TTACGCCAGC 
CCCTACGCGT ACGCGGCCAG CGACTCCGAG TACTCGGCCG AGTGCGAGTC CCTGTTCCAC 
TCCACTGTGC TGGACACCAG CGAGGACGAG CGCAGCAACT ACACCACCAA CTGCTTCGGC 
GACAGCGAGT CCAGCGTGAG CGAGGGCGAC TTCGTGGGCG ACAGCACGAG CACCAGCGAC 
TCGGAGGAGA GCGGGGGCTT GATATGGTCC CAGTTCGTGC AGACGCTCCC CCTGCAGCCG 
GTCCCGGCCT CCGACCTTCG CCACAACCCC ACCAAAACCT TCGTCAAGAT TAAGGCCTCG 
CACAACCTCA AGAAGAAGAT CCTCCGCTTT CGATCGGGCT CTTTGAAACT GATGACGACG 
GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015320648.1
CDS115..2640
Translation

Target ORF information:

RefSeq Version XM_015465162.1
Organism Bos taurus(cattle)
Definition Bos taurus dishevelled-binding antagonist of beta-catenin 1 (DACT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015465162.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATGGCT GGAATATAAA 
GAAGGCCATT GTGAAGACCA GGCCTCAAGG GCAGTTGACT GTTCCCCCTC CACACCACAA 
TCTAATTCCC TTGCTGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGCGA TCTGGTGTCT 
AAAAATGGGA ATGATGTGTA TCGCTACCCC AGTCCACTTC ATGCCGTGGC CGTGCAGAGC 
CCAATGTTCC TCCTTTGCCT GACTGGCAAC CCACTGAGGG AAGAGGAGCG GCTTGGTAAC 
CATGCCAATG ACGTTTGTGC GGTATCTGAG CTGGACGCCG TCAAGACAGA CACTAACTCA 
CCATCTCCAA GCAGTTTGTG GTCTGCTCCC CATCCTTCAT CCAGCAAGAA GATGGATGGT 
TACATTCTGA GCCTGGTCCA GAAAAAAACC CACCCAGTAA GGACCAACAA ACCGAGGACC 
AGTGTGAACG CTGACCCCAC GAAGGGGCTT CTGAGGAATG GGAGCGTTTG TGTCAGAGTG 
ACTGGTGGTG TCTCACAGGG CAACAATGGG AACCTTAAGA ATTCGAAACA GGTGCCTTTG 
CCCTCCGGAG GCATCCCTTC TTTGGACAGT GGGGGCTTCT CCCCACCAAA ACAGTGGTCA 
AAAGAATCAA AGCCAGAACC TCTGGAAAGC AAGAGGTTGC CCGCGCCTGA AGGCTGTTCT 
CCAGGCACTG CTACCGAACT TCAAGGTAAG CATTTGCCCA AAAATGCCAA GCCCGTCTCC 
CAGGAACATG CGAGGTGTCC CCCCGCTGGG ACAGGGGAGT CCTCTAAGGA AAGCGGGCAG 
ATCCCAGCTG CCTCTCCGAA AGAGAGCCCT GGGAGGGTCC TCGCACCGCT GCAAGAGAAC 
AAAGTGGTCC AGCCACTGAA AAAGGTGCCA CAGAAAAACA GCCCGCAGGC AGGCACTCTG 
GCGCCCCCTC CCGCCGCCTC AGCCCTGCTG CCCCCCACTT TCCCGGCGGA AGAGCGGCCG 
GCCCTGGATT TCAGAAGCGA GGGCTCTTCT TCTCAGAGCC TGGAGGAAGG GCCGCCGGGG 
AAGGCGCCGC CCGTCCTGGC GCCGCCTCCG GGGGTCAGGC CACCCCGGGG TGCGCGCCCC 
GCGGCCGCCC CGAGGGGCTC CGGCGTGAAG CACCGGGCGC TGGCCGTCCA CGGGCCGGAA 
AGTGCTCTGC CCCCCGTGAG GGAGAAGGCC CGGGTGGCCG GCAAGAAGTG TCGGTTCCCC 
GACGACACGG ATACAAATAA GAAACTGCGG CGAGCCCCGG CTAAGGCGCG GCGGGGCGGG 
GGCGGCCAGC CGGACGCGGG GCTCCCCAGC CGGCCTCTGG GGGCGGGCCA CCGCGCAGGG 
AGCCGGGCGC ACGGCCACGG CCACGGCCGG GAGGCGGTGG TGGCCAAGCC CAAGCACAAG 
CGCACCGACT CGCGGCGGTG GAGGTCGGCC GCCGAGGTGT CGTACGAGGA GGCGCTGCGG 
CGCGCGCGGC GGGGCCGACG GGAGCCCGCG GGGCTGTTTG CGGCCGGGCC TTACGCCAGC 
CCCTACGCGT ACGCGGCCAG CGACTCCGAG TACTCGGCCG AGTGCGAGTC CCTGTTCCAC 
TCCACTGTGC TGGACACCAG CGAGGACGAG CGCAGCAACT ACACCACCAA CTGCTTCGGC 
GACAGCGAGT CCAGCGTGAG CGAGGGCGAC TTCGTGGGCG ACAGCACGAG CACCAGCGAC 
TCGGAGGAGA GCGGGGGCTT GATATGGTCC CAGTTCGTGC AGACGCTCCC CCTGCAGCCG 
GTCCCGGCCT CCGACCTTCG CCACAACCCC ACCAAAACCT TCGTCAAGAT TAAGGCCTCG 
CACAACCTCA AGAAGAAGAT CCTCCGCTTT CGATCGGGCT CTTTGAAACT GATGACGACG 
GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa23429
Clone ID Related Accession (Same CDS sequence) XM_002690960.5 , XM_015465162.1 , XM_002690960.4
Accession Version XM_002690960.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2526bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product dapper homolog 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATGGCT GGAATATAAA 
GAAGGCCATT GTGAAGACCA GGCCTCAAGG GCAGTTGACT GTTCCCCCTC CACACCACAA 
TCTAATTCCC TTGCTGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGCGA TCTGGTGTCT 
AAAAATGGGA ATGATGTGTA TCGCTACCCC AGTCCACTTC ATGCCGTGGC CGTGCAGAGC 
CCAATGTTCC TCCTTTGCCT GACTGGCAAC CCACTGAGGG AAGAGGAGCG GCTTGGTAAC 
CATGCCAATG ACGTTTGTGC GGTATCTGAG CTGGACGCCG TCAAGACAGA CACTAACTCA 
CCATCTCCAA GCAGTTTGTG GTCTGCTCCC CATCCTTCAT CCAGCAAGAA GATGGATGGT 
TACATTCTGA GCCTGGTCCA GAAAAAAACC CACCCAGTAA GGACCAACAA ACCGAGGACC 
AGTGTGAACG CTGACCCCAC GAAGGGGCTT CTGAGGAATG GGAGCGTTTG TGTCAGAGTG 
ACTGGTGGTG TCTCACAGGG CAACAATGGG AACCTTAAGA ATTCGAAACA GGTGCCTTTG 
CCCTCCGGAG GCATCCCTTC TTTGGACAGT GGGGGCTTCT CCCCACCAAA ACAGTGGTCA 
AAAGAATCAA AGCCAGAACC TCTGGAAAGC AAGAGGTTGC CCGCGCCTGA AGGCTGTTCT 
CCAGGCACTG CTACCGAACT TCAAGGTAAG CATTTGCCCA AAAATGCCAA GCCCGTCTCC 
CAGGAACATG CGAGGTGTCC CCCCGCTGGG ACAGGGGAGT CCTCTAAGGA AAGCGGGCAG 
ATCCCAGCTG CCTCTCCGAA AGAGAGCCCT GGGAGGGTCC TCGCACCGCT GCAAGAGAAC 
AAAGTGGTCC AGCCACTGAA AAAGGTGCCA CAGAAAAACA GCCCGCAGGC AGGCACTCTG 
GCGCCCCCTC CCGCCGCCTC AGCCCTGCTG CCCCCCACTT TCCCGGCGGA AGAGCGGCCG 
GCCCTGGATT TCAGAAGCGA GGGCTCTTCT TCTCAGAGCC TGGAGGAAGG GCCGCCGGGG 
AAGGCGCCGC CCGTCCTGGC GCCGCCTCCG GGGGTCAGGC CACCCCGGGG TGCGCGCCCC 
GCGGCCGCCC CGAGGGGCTC CGGCGTGAAG CACCGGGCGC TGGCCGTCCA CGGGCCGGAA 
AGTGCTCTGC CCCCCGTGAG GGAGAAGGCC CGGGTGGCCG GCAAGAAGTG TCGGTTCCCC 
GACGACACGG ATACAAATAA GAAACTGCGG CGAGCCCCGG CTAAGGCGCG GCGGGGCGGG 
GGCGGCCAGC CGGACGCGGG GCTCCCCAGC CGGCCTCTGG GGGCGGGCCA CCGCGCAGGG 
AGCCGGGCGC ACGGCCACGG CCACGGCCGG GAGGCGGTGG TGGCCAAGCC CAAGCACAAG 
CGCACCGACT CGCGGCGGTG GAGGTCGGCC GCCGAGGTGT CGTACGAGGA GGCGCTGCGG 
CGCGCGCGGC GGGGCCGACG GGAGCCCGCG GGGCTGTTTG CGGCCGGGCC TTACGCCAGC 
CCCTACGCGT ACGCGGCCAG CGACTCCGAG TACTCGGCCG AGTGCGAGTC CCTGTTCCAC 
TCCACTGTGC TGGACACCAG CGAGGACGAG CGCAGCAACT ACACCACCAA CTGCTTCGGC 
GACAGCGAGT CCAGCGTGAG CGAGGGCGAC TTCGTGGGCG ACAGCACGAG CACCAGCGAC 
TCGGAGGAGA GCGGGGGCTT GATATGGTCC CAGTTCGTGC AGACGCTCCC CCTGCAGCCG 
GTCCCGGCCT CCGACCTTCG CCACAACCCC ACCAAAACCT TCGTCAAGAT TAAGGCCTCG 
CACAACCTCA AGAAGAAGAT CCTCCGCTTT CGATCGGGCT CTTTGAAACT GATGACGACG 
GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002691006.1
CDS115..2640
Translation

Target ORF information:

RefSeq Version XM_002690960.4
Organism Bos taurus(cattle)
Definition Bos taurus dishevelled-binding antagonist of beta-catenin 1 (DACT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002690960.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATGGCT GGAATATAAA 
GAAGGCCATT GTGAAGACCA GGCCTCAAGG GCAGTTGACT GTTCCCCCTC CACACCACAA 
TCTAATTCCC TTGCTGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGCGA TCTGGTGTCT 
AAAAATGGGA ATGATGTGTA TCGCTACCCC AGTCCACTTC ATGCCGTGGC CGTGCAGAGC 
CCAATGTTCC TCCTTTGCCT GACTGGCAAC CCACTGAGGG AAGAGGAGCG GCTTGGTAAC 
CATGCCAATG ACGTTTGTGC GGTATCTGAG CTGGACGCCG TCAAGACAGA CACTAACTCA 
CCATCTCCAA GCAGTTTGTG GTCTGCTCCC CATCCTTCAT CCAGCAAGAA GATGGATGGT 
TACATTCTGA GCCTGGTCCA GAAAAAAACC CACCCAGTAA GGACCAACAA ACCGAGGACC 
AGTGTGAACG CTGACCCCAC GAAGGGGCTT CTGAGGAATG GGAGCGTTTG TGTCAGAGTG 
ACTGGTGGTG TCTCACAGGG CAACAATGGG AACCTTAAGA ATTCGAAACA GGTGCCTTTG 
CCCTCCGGAG GCATCCCTTC TTTGGACAGT GGGGGCTTCT CCCCACCAAA ACAGTGGTCA 
AAAGAATCAA AGCCAGAACC TCTGGAAAGC AAGAGGTTGC CCGCGCCTGA AGGCTGTTCT 
CCAGGCACTG CTACCGAACT TCAAGGTAAG CATTTGCCCA AAAATGCCAA GCCCGTCTCC 
CAGGAACATG CGAGGTGTCC CCCCGCTGGG ACAGGGGAGT CCTCTAAGGA AAGCGGGCAG 
ATCCCAGCTG CCTCTCCGAA AGAGAGCCCT GGGAGGGTCC TCGCACCGCT GCAAGAGAAC 
AAAGTGGTCC AGCCACTGAA AAAGGTGCCA CAGAAAAACA GCCCGCAGGC AGGCACTCTG 
GCGCCCCCTC CCGCCGCCTC AGCCCTGCTG CCCCCCACTT TCCCGGCGGA AGAGCGGCCG 
GCCCTGGATT TCAGAAGCGA GGGCTCTTCT TCTCAGAGCC TGGAGGAAGG GCCGCCGGGG 
AAGGCGCCGC CCGTCCTGGC GCCGCCTCCG GGGGTCAGGC CACCCCGGGG TGCGCGCCCC 
GCGGCCGCCC CGAGGGGCTC CGGCGTGAAG CACCGGGCGC TGGCCGTCCA CGGGCCGGAA 
AGTGCTCTGC CCCCCGTGAG GGAGAAGGCC CGGGTGGCCG GCAAGAAGTG TCGGTTCCCC 
GACGACACGG ATACAAATAA GAAACTGCGG CGAGCCCCGG CTAAGGCGCG GCGGGGCGGG 
GGCGGCCAGC CGGACGCGGG GCTCCCCAGC CGGCCTCTGG GGGCGGGCCA CCGCGCAGGG 
AGCCGGGCGC ACGGCCACGG CCACGGCCGG GAGGCGGTGG TGGCCAAGCC CAAGCACAAG 
CGCACCGACT CGCGGCGGTG GAGGTCGGCC GCCGAGGTGT CGTACGAGGA GGCGCTGCGG 
CGCGCGCGGC GGGGCCGACG GGAGCCCGCG GGGCTGTTTG CGGCCGGGCC TTACGCCAGC 
CCCTACGCGT ACGCGGCCAG CGACTCCGAG TACTCGGCCG AGTGCGAGTC CCTGTTCCAC 
TCCACTGTGC TGGACACCAG CGAGGACGAG CGCAGCAACT ACACCACCAA CTGCTTCGGC 
GACAGCGAGT CCAGCGTGAG CGAGGGCGAC TTCGTGGGCG ACAGCACGAG CACCAGCGAC 
TCGGAGGAGA GCGGGGGCTT GATATGGTCC CAGTTCGTGC AGACGCTCCC CCTGCAGCCG 
GTCCCGGCCT CCGACCTTCG CCACAACCCC ACCAAAACCT TCGTCAAGAT TAAGGCCTCG 
CACAACCTCA AGAAGAAGAT CCTCCGCTTT CGATCGGGCT CTTTGAAACT GATGACGACG 
GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa23430
Clone ID Related Accession (Same CDS sequence) XM_015465163.1 , XM_002690959.5 , XM_002690959.4
Accession Version XM_015465163.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product dapper homolog 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGCGAT 
CTGGTGTCTA AAAATGGGAA TGATGTGTAT CGCTACCCCA GTCCACTTCA TGCCGTGGCC 
GTGCAGAGCC CAATGTTCCT CCTTTGCCTG ACTGGCAACC CACTGAGGGA AGAGGAGCGG 
CTTGGTAACC ATGCCAATGA CGTTTGTGCG GTATCTGAGC TGGACGCCGT CAAGACAGAC 
ACTAACTCAC CATCTCCAAG CAGTTTGTGG TCTGCTCCCC ATCCTTCATC CAGCAAGAAG 
ATGGATGGTT ACATTCTGAG CCTGGTCCAG AAAAAAACCC ACCCAGTAAG GACCAACAAA 
CCGAGGACCA GTGTGAACGC TGACCCCACG AAGGGGCTTC TGAGGAATGG GAGCGTTTGT 
GTCAGAGTGA CTGGTGGTGT CTCACAGGGC AACAATGGGA ACCTTAAGAA TTCGAAACAG 
GTGCCTTTGC CCTCCGGAGG CATCCCTTCT TTGGACAGTG GGGGCTTCTC CCCACCAAAA 
CAGTGGTCAA AAGAATCAAA GCCAGAACCT CTGGAAAGCA AGAGGTTGCC CGCGCCTGAA 
GGCTGTTCTC CAGGCACTGC TACCGAACTT CAAGGTAAGC ATTTGCCCAA AAATGCCAAG 
CCCGTCTCCC AGGAACATGC GAGGTGTCCC CCCGCTGGGA CAGGGGAGTC CTCTAAGGAA 
AGCGGGCAGA TCCCAGCTGC CTCTCCGAAA GAGAGCCCTG GGAGGGTCCT CGCACCGCTG 
CAAGAGAACA AAGTGGTCCA GCCACTGAAA AAGGTGCCAC AGAAAAACAG CCCGCAGGCA 
GGCACTCTGG CGCCCCCTCC CGCCGCCTCA GCCCTGCTGC CCCCCACTTT CCCGGCGGAA 
GAGCGGCCGG CCCTGGATTT CAGAAGCGAG GGCTCTTCTT CTCAGAGCCT GGAGGAAGGG 
CCGCCGGGGA AGGCGCCGCC CGTCCTGGCG CCGCCTCCGG GGGTCAGGCC ACCCCGGGGT 
GCGCGCCCCG CGGCCGCCCC GAGGGGCTCC GGCGTGAAGC ACCGGGCGCT GGCCGTCCAC 
GGGCCGGAAA GTGCTCTGCC CCCCGTGAGG GAGAAGGCCC GGGTGGCCGG CAAGAAGTGT 
CGGTTCCCCG ACGACACGGA TACAAATAAG AAACTGCGGC GAGCCCCGGC TAAGGCGCGG 
CGGGGCGGGG GCGGCCAGCC GGACGCGGGG CTCCCCAGCC GGCCTCTGGG GGCGGGCCAC 
CGCGCAGGGA GCCGGGCGCA CGGCCACGGC CACGGCCGGG AGGCGGTGGT GGCCAAGCCC 
AAGCACAAGC GCACCGACTC GCGGCGGTGG AGGTCGGCCG CCGAGGTGTC GTACGAGGAG 
GCGCTGCGGC GCGCGCGGCG GGGCCGACGG GAGCCCGCGG GGCTGTTTGC GGCCGGGCCT 
TACGCCAGCC CCTACGCGTA CGCGGCCAGC GACTCCGAGT ACTCGGCCGA GTGCGAGTCC 
CTGTTCCACT CCACTGTGCT GGACACCAGC GAGGACGAGC GCAGCAACTA CACCACCAAC 
TGCTTCGGCG ACAGCGAGTC CAGCGTGAGC GAGGGCGACT TCGTGGGCGA CAGCACGAGC 
ACCAGCGACT CGGAGGAGAG CGGGGGCTTG ATATGGTCCC AGTTCGTGCA GACGCTCCCC 
CTGCAGCCGG TCCCGGCCTC CGACCTTCGC CACAACCCCA CCAAAACCTT CGTCAAGATT 
AAGGCCTCGC ACAACCTCAA GAAGAAGATC CTCCGCTTTC GATCGGGCTC TTTGAAACTG 
ATGACGACGG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015320649.1
CDS108..2522
Translation

Target ORF information:

RefSeq Version XM_015465163.1
Organism Bos taurus(cattle)
Definition Bos taurus dishevelled-binding antagonist of beta-catenin 1 (DACT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015465163.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGCGAT 
CTGGTGTCTA AAAATGGGAA TGATGTGTAT CGCTACCCCA GTCCACTTCA TGCCGTGGCC 
GTGCAGAGCC CAATGTTCCT CCTTTGCCTG ACTGGCAACC CACTGAGGGA AGAGGAGCGG 
CTTGGTAACC ATGCCAATGA CGTTTGTGCG GTATCTGAGC TGGACGCCGT CAAGACAGAC 
ACTAACTCAC CATCTCCAAG CAGTTTGTGG TCTGCTCCCC ATCCTTCATC CAGCAAGAAG 
ATGGATGGTT ACATTCTGAG CCTGGTCCAG AAAAAAACCC ACCCAGTAAG GACCAACAAA 
CCGAGGACCA GTGTGAACGC TGACCCCACG AAGGGGCTTC TGAGGAATGG GAGCGTTTGT 
GTCAGAGTGA CTGGTGGTGT CTCACAGGGC AACAATGGGA ACCTTAAGAA TTCGAAACAG 
GTGCCTTTGC CCTCCGGAGG CATCCCTTCT TTGGACAGTG GGGGCTTCTC CCCACCAAAA 
CAGTGGTCAA AAGAATCAAA GCCAGAACCT CTGGAAAGCA AGAGGTTGCC CGCGCCTGAA 
GGCTGTTCTC CAGGCACTGC TACCGAACTT CAAGGTAAGC ATTTGCCCAA AAATGCCAAG 
CCCGTCTCCC AGGAACATGC GAGGTGTCCC CCCGCTGGGA CAGGGGAGTC CTCTAAGGAA 
AGCGGGCAGA TCCCAGCTGC CTCTCCGAAA GAGAGCCCTG GGAGGGTCCT CGCACCGCTG 
CAAGAGAACA AAGTGGTCCA GCCACTGAAA AAGGTGCCAC AGAAAAACAG CCCGCAGGCA 
GGCACTCTGG CGCCCCCTCC CGCCGCCTCA GCCCTGCTGC CCCCCACTTT CCCGGCGGAA 
GAGCGGCCGG CCCTGGATTT CAGAAGCGAG GGCTCTTCTT CTCAGAGCCT GGAGGAAGGG 
CCGCCGGGGA AGGCGCCGCC CGTCCTGGCG CCGCCTCCGG GGGTCAGGCC ACCCCGGGGT 
GCGCGCCCCG CGGCCGCCCC GAGGGGCTCC GGCGTGAAGC ACCGGGCGCT GGCCGTCCAC 
GGGCCGGAAA GTGCTCTGCC CCCCGTGAGG GAGAAGGCCC GGGTGGCCGG CAAGAAGTGT 
CGGTTCCCCG ACGACACGGA TACAAATAAG AAACTGCGGC GAGCCCCGGC TAAGGCGCGG 
CGGGGCGGGG GCGGCCAGCC GGACGCGGGG CTCCCCAGCC GGCCTCTGGG GGCGGGCCAC 
CGCGCAGGGA GCCGGGCGCA CGGCCACGGC CACGGCCGGG AGGCGGTGGT GGCCAAGCCC 
AAGCACAAGC GCACCGACTC GCGGCGGTGG AGGTCGGCCG CCGAGGTGTC GTACGAGGAG 
GCGCTGCGGC GCGCGCGGCG GGGCCGACGG GAGCCCGCGG GGCTGTTTGC GGCCGGGCCT 
TACGCCAGCC CCTACGCGTA CGCGGCCAGC GACTCCGAGT ACTCGGCCGA GTGCGAGTCC 
CTGTTCCACT CCACTGTGCT GGACACCAGC GAGGACGAGC GCAGCAACTA CACCACCAAC 
TGCTTCGGCG ACAGCGAGTC CAGCGTGAGC GAGGGCGACT TCGTGGGCGA CAGCACGAGC 
ACCAGCGACT CGGAGGAGAG CGGGGGCTTG ATATGGTCCC AGTTCGTGCA GACGCTCCCC 
CTGCAGCCGG TCCCGGCCTC CGACCTTCGC CACAACCCCA CCAAAACCTT CGTCAAGATT 
AAGGCCTCGC ACAACCTCAA GAAGAAGATC CTCCGCTTTC GATCGGGCTC TTTGAAACTG 
ATGACGACGG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa23430
Clone ID Related Accession (Same CDS sequence) XM_015465163.1 , XM_002690959.5 , XM_002690959.4
Accession Version XM_002690959.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product dapper homolog 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690959.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGCGAT 
CTGGTGTCTA AAAATGGGAA TGATGTGTAT CGCTACCCCA GTCCACTTCA TGCCGTGGCC 
GTGCAGAGCC CAATGTTCCT CCTTTGCCTG ACTGGCAACC CACTGAGGGA AGAGGAGCGG 
CTTGGTAACC ATGCCAATGA CGTTTGTGCG GTATCTGAGC TGGACGCCGT CAAGACAGAC 
ACTAACTCAC CATCTCCAAG CAGTTTGTGG TCTGCTCCCC ATCCTTCATC CAGCAAGAAG 
ATGGATGGTT ACATTCTGAG CCTGGTCCAG AAAAAAACCC ACCCAGTAAG GACCAACAAA 
CCGAGGACCA GTGTGAACGC TGACCCCACG AAGGGGCTTC TGAGGAATGG GAGCGTTTGT 
GTCAGAGTGA CTGGTGGTGT CTCACAGGGC AACAATGGGA ACCTTAAGAA TTCGAAACAG 
GTGCCTTTGC CCTCCGGAGG CATCCCTTCT TTGGACAGTG GGGGCTTCTC CCCACCAAAA 
CAGTGGTCAA AAGAATCAAA GCCAGAACCT CTGGAAAGCA AGAGGTTGCC CGCGCCTGAA 
GGCTGTTCTC CAGGCACTGC TACCGAACTT CAAGGTAAGC ATTTGCCCAA AAATGCCAAG 
CCCGTCTCCC AGGAACATGC GAGGTGTCCC CCCGCTGGGA CAGGGGAGTC CTCTAAGGAA 
AGCGGGCAGA TCCCAGCTGC CTCTCCGAAA GAGAGCCCTG GGAGGGTCCT CGCACCGCTG 
CAAGAGAACA AAGTGGTCCA GCCACTGAAA AAGGTGCCAC AGAAAAACAG CCCGCAGGCA 
GGCACTCTGG CGCCCCCTCC CGCCGCCTCA GCCCTGCTGC CCCCCACTTT CCCGGCGGAA 
GAGCGGCCGG CCCTGGATTT CAGAAGCGAG GGCTCTTCTT CTCAGAGCCT GGAGGAAGGG 
CCGCCGGGGA AGGCGCCGCC CGTCCTGGCG CCGCCTCCGG GGGTCAGGCC ACCCCGGGGT 
GCGCGCCCCG CGGCCGCCCC GAGGGGCTCC GGCGTGAAGC ACCGGGCGCT GGCCGTCCAC 
GGGCCGGAAA GTGCTCTGCC CCCCGTGAGG GAGAAGGCCC GGGTGGCCGG CAAGAAGTGT 
CGGTTCCCCG ACGACACGGA TACAAATAAG AAACTGCGGC GAGCCCCGGC TAAGGCGCGG 
CGGGGCGGGG GCGGCCAGCC GGACGCGGGG CTCCCCAGCC GGCCTCTGGG GGCGGGCCAC 
CGCGCAGGGA GCCGGGCGCA CGGCCACGGC CACGGCCGGG AGGCGGTGGT GGCCAAGCCC 
AAGCACAAGC GCACCGACTC GCGGCGGTGG AGGTCGGCCG CCGAGGTGTC GTACGAGGAG 
GCGCTGCGGC GCGCGCGGCG GGGCCGACGG GAGCCCGCGG GGCTGTTTGC GGCCGGGCCT 
TACGCCAGCC CCTACGCGTA CGCGGCCAGC GACTCCGAGT ACTCGGCCGA GTGCGAGTCC 
CTGTTCCACT CCACTGTGCT GGACACCAGC GAGGACGAGC GCAGCAACTA CACCACCAAC 
TGCTTCGGCG ACAGCGAGTC CAGCGTGAGC GAGGGCGACT TCGTGGGCGA CAGCACGAGC 
ACCAGCGACT CGGAGGAGAG CGGGGGCTTG ATATGGTCCC AGTTCGTGCA GACGCTCCCC 
CTGCAGCCGG TCCCGGCCTC CGACCTTCGC CACAACCCCA CCAAAACCTT CGTCAAGATT 
AAGGCCTCGC ACAACCTCAA GAAGAAGATC CTCCGCTTTC GATCGGGCTC TTTGAAACTG 
ATGACGACGG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002691005.1
CDS96..2510
Translation

Target ORF information:

RefSeq Version XM_002690959.5
Organism Bos taurus(cattle)
Definition Bos taurus dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002690959.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGCGAT 
CTGGTGTCTA AAAATGGGAA TGATGTGTAT CGCTACCCCA GTCCACTTCA TGCCGTGGCC 
GTGCAGAGCC CAATGTTCCT CCTTTGCCTG ACTGGCAACC CACTGAGGGA AGAGGAGCGG 
CTTGGTAACC ATGCCAATGA CGTTTGTGCG GTATCTGAGC TGGACGCCGT CAAGACAGAC 
ACTAACTCAC CATCTCCAAG CAGTTTGTGG TCTGCTCCCC ATCCTTCATC CAGCAAGAAG 
ATGGATGGTT ACATTCTGAG CCTGGTCCAG AAAAAAACCC ACCCAGTAAG GACCAACAAA 
CCGAGGACCA GTGTGAACGC TGACCCCACG AAGGGGCTTC TGAGGAATGG GAGCGTTTGT 
GTCAGAGTGA CTGGTGGTGT CTCACAGGGC AACAATGGGA ACCTTAAGAA TTCGAAACAG 
GTGCCTTTGC CCTCCGGAGG CATCCCTTCT TTGGACAGTG GGGGCTTCTC CCCACCAAAA 
CAGTGGTCAA AAGAATCAAA GCCAGAACCT CTGGAAAGCA AGAGGTTGCC CGCGCCTGAA 
GGCTGTTCTC CAGGCACTGC TACCGAACTT CAAGGTAAGC ATTTGCCCAA AAATGCCAAG 
CCCGTCTCCC AGGAACATGC GAGGTGTCCC CCCGCTGGGA CAGGGGAGTC CTCTAAGGAA 
AGCGGGCAGA TCCCAGCTGC CTCTCCGAAA GAGAGCCCTG GGAGGGTCCT CGCACCGCTG 
CAAGAGAACA AAGTGGTCCA GCCACTGAAA AAGGTGCCAC AGAAAAACAG CCCGCAGGCA 
GGCACTCTGG CGCCCCCTCC CGCCGCCTCA GCCCTGCTGC CCCCCACTTT CCCGGCGGAA 
GAGCGGCCGG CCCTGGATTT CAGAAGCGAG GGCTCTTCTT CTCAGAGCCT GGAGGAAGGG 
CCGCCGGGGA AGGCGCCGCC CGTCCTGGCG CCGCCTCCGG GGGTCAGGCC ACCCCGGGGT 
GCGCGCCCCG CGGCCGCCCC GAGGGGCTCC GGCGTGAAGC ACCGGGCGCT GGCCGTCCAC 
GGGCCGGAAA GTGCTCTGCC CCCCGTGAGG GAGAAGGCCC GGGTGGCCGG CAAGAAGTGT 
CGGTTCCCCG ACGACACGGA TACAAATAAG AAACTGCGGC GAGCCCCGGC TAAGGCGCGG 
CGGGGCGGGG GCGGCCAGCC GGACGCGGGG CTCCCCAGCC GGCCTCTGGG GGCGGGCCAC 
CGCGCAGGGA GCCGGGCGCA CGGCCACGGC CACGGCCGGG AGGCGGTGGT GGCCAAGCCC 
AAGCACAAGC GCACCGACTC GCGGCGGTGG AGGTCGGCCG CCGAGGTGTC GTACGAGGAG 
GCGCTGCGGC GCGCGCGGCG GGGCCGACGG GAGCCCGCGG GGCTGTTTGC GGCCGGGCCT 
TACGCCAGCC CCTACGCGTA CGCGGCCAGC GACTCCGAGT ACTCGGCCGA GTGCGAGTCC 
CTGTTCCACT CCACTGTGCT GGACACCAGC GAGGACGAGC GCAGCAACTA CACCACCAAC 
TGCTTCGGCG ACAGCGAGTC CAGCGTGAGC GAGGGCGACT TCGTGGGCGA CAGCACGAGC 
ACCAGCGACT CGGAGGAGAG CGGGGGCTTG ATATGGTCCC AGTTCGTGCA GACGCTCCCC 
CTGCAGCCGG TCCCGGCCTC CGACCTTCGC CACAACCCCA CCAAAACCTT CGTCAAGATT 
AAGGCCTCGC ACAACCTCAA GAAGAAGATC CTCCGCTTTC GATCGGGCTC TTTGAAACTG 
ATGACGACGG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa23430
Clone ID Related Accession (Same CDS sequence) XM_015465163.1 , XM_002690959.5 , XM_002690959.4
Accession Version XM_002690959.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product dapper homolog 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690959.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGCGAT 
CTGGTGTCTA AAAATGGGAA TGATGTGTAT CGCTACCCCA GTCCACTTCA TGCCGTGGCC 
GTGCAGAGCC CAATGTTCCT CCTTTGCCTG ACTGGCAACC CACTGAGGGA AGAGGAGCGG 
CTTGGTAACC ATGCCAATGA CGTTTGTGCG GTATCTGAGC TGGACGCCGT CAAGACAGAC 
ACTAACTCAC CATCTCCAAG CAGTTTGTGG TCTGCTCCCC ATCCTTCATC CAGCAAGAAG 
ATGGATGGTT ACATTCTGAG CCTGGTCCAG AAAAAAACCC ACCCAGTAAG GACCAACAAA 
CCGAGGACCA GTGTGAACGC TGACCCCACG AAGGGGCTTC TGAGGAATGG GAGCGTTTGT 
GTCAGAGTGA CTGGTGGTGT CTCACAGGGC AACAATGGGA ACCTTAAGAA TTCGAAACAG 
GTGCCTTTGC CCTCCGGAGG CATCCCTTCT TTGGACAGTG GGGGCTTCTC CCCACCAAAA 
CAGTGGTCAA AAGAATCAAA GCCAGAACCT CTGGAAAGCA AGAGGTTGCC CGCGCCTGAA 
GGCTGTTCTC CAGGCACTGC TACCGAACTT CAAGGTAAGC ATTTGCCCAA AAATGCCAAG 
CCCGTCTCCC AGGAACATGC GAGGTGTCCC CCCGCTGGGA CAGGGGAGTC CTCTAAGGAA 
AGCGGGCAGA TCCCAGCTGC CTCTCCGAAA GAGAGCCCTG GGAGGGTCCT CGCACCGCTG 
CAAGAGAACA AAGTGGTCCA GCCACTGAAA AAGGTGCCAC AGAAAAACAG CCCGCAGGCA 
GGCACTCTGG CGCCCCCTCC CGCCGCCTCA GCCCTGCTGC CCCCCACTTT CCCGGCGGAA 
GAGCGGCCGG CCCTGGATTT CAGAAGCGAG GGCTCTTCTT CTCAGAGCCT GGAGGAAGGG 
CCGCCGGGGA AGGCGCCGCC CGTCCTGGCG CCGCCTCCGG GGGTCAGGCC ACCCCGGGGT 
GCGCGCCCCG CGGCCGCCCC GAGGGGCTCC GGCGTGAAGC ACCGGGCGCT GGCCGTCCAC 
GGGCCGGAAA GTGCTCTGCC CCCCGTGAGG GAGAAGGCCC GGGTGGCCGG CAAGAAGTGT 
CGGTTCCCCG ACGACACGGA TACAAATAAG AAACTGCGGC GAGCCCCGGC TAAGGCGCGG 
CGGGGCGGGG GCGGCCAGCC GGACGCGGGG CTCCCCAGCC GGCCTCTGGG GGCGGGCCAC 
CGCGCAGGGA GCCGGGCGCA CGGCCACGGC CACGGCCGGG AGGCGGTGGT GGCCAAGCCC 
AAGCACAAGC GCACCGACTC GCGGCGGTGG AGGTCGGCCG CCGAGGTGTC GTACGAGGAG 
GCGCTGCGGC GCGCGCGGCG GGGCCGACGG GAGCCCGCGG GGCTGTTTGC GGCCGGGCCT 
TACGCCAGCC CCTACGCGTA CGCGGCCAGC GACTCCGAGT ACTCGGCCGA GTGCGAGTCC 
CTGTTCCACT CCACTGTGCT GGACACCAGC GAGGACGAGC GCAGCAACTA CACCACCAAC 
TGCTTCGGCG ACAGCGAGTC CAGCGTGAGC GAGGGCGACT TCGTGGGCGA CAGCACGAGC 
ACCAGCGACT CGGAGGAGAG CGGGGGCTTG ATATGGTCCC AGTTCGTGCA GACGCTCCCC 
CTGCAGCCGG TCCCGGCCTC CGACCTTCGC CACAACCCCA CCAAAACCTT CGTCAAGATT 
AAGGCCTCGC ACAACCTCAA GAAGAAGATC CTCCGCTTTC GATCGGGCTC TTTGAAACTG 
ATGACGACGG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002691005.1
CDS108..2522
Translation

Target ORF information:

RefSeq Version XM_002690959.4
Organism Bos taurus(cattle)
Definition Bos taurus dishevelled-binding antagonist of beta-catenin 1 (DACT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002690959.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAAGCCGA GTCCGACTGG GACGGCTAAG GAGCAGGAGC CGCCGGCGCC GGCCCGGGGA 
GAGCAGCGCA CGGCGGAGCC TGAGGGACGC TGGCGGGAGA AGGGCGAGGC GGACACGGAG 
CGGCAGCGCA CCCGCGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAGGAGCT GCTGGTCCGG GGCGCCCTGC GCGGCACGGG GGGTGCGGGG 
GCCGCTGCAG CCCGCGCTGG GGAGCTGCCG GGGGAGGCGG CGCAGCGCAA CCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTGCGGAAGC AATTGAATTG TTTGAGGAGA 
AGAGATGCTG GTTTGTTGAA CCAGTTGCAG GAACTCGACA AGCAGATAAG TGACCTCAGA 
CTGGATGTGG AAAAGACATC TGAAGAGCGC CTGGAGACAG ACAGCCGCCC CAGCTCAGGG 
TTTTATGAAC TGAGTGATGG GGCTTCGGGA TCCCTGTCCA ATTCCTCGAA CTCGGTCTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTCT GCAGCCCCTT GGAGGCAACC 
TTGACTATCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGCGAT 
CTGGTGTCTA AAAATGGGAA TGATGTGTAT CGCTACCCCA GTCCACTTCA TGCCGTGGCC 
GTGCAGAGCC CAATGTTCCT CCTTTGCCTG ACTGGCAACC CACTGAGGGA AGAGGAGCGG 
CTTGGTAACC ATGCCAATGA CGTTTGTGCG GTATCTGAGC TGGACGCCGT CAAGACAGAC 
ACTAACTCAC CATCTCCAAG CAGTTTGTGG TCTGCTCCCC ATCCTTCATC CAGCAAGAAG 
ATGGATGGTT ACATTCTGAG CCTGGTCCAG AAAAAAACCC ACCCAGTAAG GACCAACAAA 
CCGAGGACCA GTGTGAACGC TGACCCCACG AAGGGGCTTC TGAGGAATGG GAGCGTTTGT 
GTCAGAGTGA CTGGTGGTGT CTCACAGGGC AACAATGGGA ACCTTAAGAA TTCGAAACAG 
GTGCCTTTGC CCTCCGGAGG CATCCCTTCT TTGGACAGTG GGGGCTTCTC CCCACCAAAA 
CAGTGGTCAA AAGAATCAAA GCCAGAACCT CTGGAAAGCA AGAGGTTGCC CGCGCCTGAA 
GGCTGTTCTC CAGGCACTGC TACCGAACTT CAAGGTAAGC ATTTGCCCAA AAATGCCAAG 
CCCGTCTCCC AGGAACATGC GAGGTGTCCC CCCGCTGGGA CAGGGGAGTC CTCTAAGGAA 
AGCGGGCAGA TCCCAGCTGC CTCTCCGAAA GAGAGCCCTG GGAGGGTCCT CGCACCGCTG 
CAAGAGAACA AAGTGGTCCA GCCACTGAAA AAGGTGCCAC AGAAAAACAG CCCGCAGGCA 
GGCACTCTGG CGCCCCCTCC CGCCGCCTCA GCCCTGCTGC CCCCCACTTT CCCGGCGGAA 
GAGCGGCCGG CCCTGGATTT CAGAAGCGAG GGCTCTTCTT CTCAGAGCCT GGAGGAAGGG 
CCGCCGGGGA AGGCGCCGCC CGTCCTGGCG CCGCCTCCGG GGGTCAGGCC ACCCCGGGGT 
GCGCGCCCCG CGGCCGCCCC GAGGGGCTCC GGCGTGAAGC ACCGGGCGCT GGCCGTCCAC 
GGGCCGGAAA GTGCTCTGCC CCCCGTGAGG GAGAAGGCCC GGGTGGCCGG CAAGAAGTGT 
CGGTTCCCCG ACGACACGGA TACAAATAAG AAACTGCGGC GAGCCCCGGC TAAGGCGCGG 
CGGGGCGGGG GCGGCCAGCC GGACGCGGGG CTCCCCAGCC GGCCTCTGGG GGCGGGCCAC 
CGCGCAGGGA GCCGGGCGCA CGGCCACGGC CACGGCCGGG AGGCGGTGGT GGCCAAGCCC 
AAGCACAAGC GCACCGACTC GCGGCGGTGG AGGTCGGCCG CCGAGGTGTC GTACGAGGAG 
GCGCTGCGGC GCGCGCGGCG GGGCCGACGG GAGCCCGCGG GGCTGTTTGC GGCCGGGCCT 
TACGCCAGCC CCTACGCGTA CGCGGCCAGC GACTCCGAGT ACTCGGCCGA GTGCGAGTCC 
CTGTTCCACT CCACTGTGCT GGACACCAGC GAGGACGAGC GCAGCAACTA CACCACCAAC 
TGCTTCGGCG ACAGCGAGTC CAGCGTGAGC GAGGGCGACT TCGTGGGCGA CAGCACGAGC 
ACCAGCGACT CGGAGGAGAG CGGGGGCTTG ATATGGTCCC AGTTCGTGCA GACGCTCCCC 
CTGCAGCCGG TCCCGGCCTC CGACCTTCGC CACAACCCCA CCAAAACCTT CGTCAAGATT 
AAGGCCTCGC ACAACCTCAA GAAGAAGATC CTCCGCTTTC GATCGGGCTC TTTGAAACTG 
ATGACGACGG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

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