RASEF cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASEF
Entrez Gene ID	106884554
Full Name	RAS and EF-hand domain containing
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014936023.1	XP_014791509.1	ras and EF-hand domain-containing protein

Pathways from BioSystems
Gene Ontology

The following RASEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa57843	XM_014936023.1 Latest version!	Calidris pugnax RAS and EF-hand domain containing (RASEF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASEF ( XM_014936023.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014791509.1 Calidris pugnax RAS and EF-hand domain containing (RASEF), mRNA.

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CloneID	OCa57843
Clone ID Related Accession (Same CDS sequence)	XM_014936023.1
Accession Version	XM_014936023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	ras and EF-hand domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090766.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCG AGACGGCGGG GGACGAGCTG TCCCGCCTGC GCGCCCTCTT CGTGGCCTGC 
GACGCCAGCG GCTCGGGCCG CATCGAGCGG GAGGACTTCG CGGCGCTCTG CGCCGAGCTG 
CGGGTGCGGC CGGCCGAGGC CGAGGCGATC TTCCAGCGCC TCGACAGCGA CCACGACGGG 
GCCATCACCT TCCCGGAGTT TGCCCGCGGC TTCCGCGGCG CCACCCGACG GCGGCCCGGC 
GGCGGCGAGA CGGAGGGGGA CGAGAAGGAG GAGGAGGAGG AGGAGGAGGA GGCGGCGGCG 
GCGTGGACCG CCGCGGGGAT AGAGCAGCCC TGGCGGGACT TCGAGGTGCG GCTCGGGGAC 
GAGGCGAGGT ACATCCCCAG ACAAGAGCAG GTCAGTGTTC TGTATCAGAA CATACACATA 
GCGGAACCAA GATTAATCCA GCCGTACGAA CATGTCATTA AGAACTTCAT CCGAGAGATC 
AAGCTTCAAA GCACAGAGAT GGAAACTTTG GCCATTGCCG TAAAAAGAGC TCAGGATAAA 
GCGGCAGTTC AGCTCAGTGA GCTGGAGGAA GAGATGGAAC AACGGATACA GGCTGCAGAG 
CACAAAGTTA AAAAGGAAGA GAAGCGAAAA GCTGAAGAAG CACTAAATGA GCTAAAGCGC 
CAGTATGATA CTGAAGTTGG GGATCTTCAA GTGACAATAA AAAAACTTAA AAAGTTGGAG 
GAGCAATCCA AAAACGTAAA TCACAGGGAA GATGTGATTG AATTGAAAAA AAGAATGCAT 
GATATGTTGC TGGAAAATCA GAAACTTAAG AAAGATCTTT TAGAAGCACA AACAAATATA 
GCTTTTCTAC AGAGTGAATT AGATAGCTTG AAAAGCGAGT ATGCAGATCA GACTTTGAAC 
ACGGAGAGAG ACCTAGAAAT GATTCGAGAA TATACTGAAG ACAGAGATAA TCTGGAACGA 
CAAATTGAAA TACTTCAATC AGCTAATAGG AAGCTACATG ATAGCAACGA TGGTTTAAGA 
AGTGCGCTTG AAAACAGTTT GACCAAGTAC AACAGATCAT TGAGGTTAGC AAACACCTCA 
CCAGGAAGTA CCATTTCTAG AAGCAGTCCT AAATTTAATG GTGGACAGTC TCCTCCAAAC 
CCACGGTATG ATAGATCATC TCATTCCTCT TGTGTGGATG AAGATTATGA TTCTTTAGCC 
CTTTGTGATC CTATGCAAAG GATAAACTGT GAAGTTGACA GCTTACCTGA GAGCTGTTTT 
GACAGTGGTT TGTCTACCCT GAGAGATTCA AATGAGTATG ATTCAGAAGT GGAATACAGG 
CATCAAAGGA TTTTTCAAAG GTCACAGTGT ATGCAAGAAA GCTTTGGTGG TGATGCTTCT 
GATACAGATG TTCCGGAGAT CAGGGATGAA GAGGCATACA GTCCTGATAA CAGCAGTACA 
GTATTAGACT GGAAGCCCTC ACGGCCAGTA AGTCGAAGCA GCTCAGTTGC TTCAACGAAG 
AAATACATAT CTGCTCTCAC CCCTCAGTCA GATGCGGGCG AATGTGCTCA GAAATACCCC 
AGCTCGGAGA AGGCCTACAA GATTGTTCTC GCCGGAGACG CAGCAGTGGG AAAGTCCAGT 
TTCCTTATGA GACTTTGCAA GAATGAATTT CGAGGAAACA CCAGTGCAAC CCTGGGTGTG 
GATTTTCAAA TGAAAAGACT AATTGTTGAT GGAGAACCTA CAGTGCTACA ACTGTGGGAC 
ACAGCTGGGC AGGAGAGATT CCGGAGTATT GCTAAATCAT ACTTCAGAAG AGCGGATGGC 
GTCTTACTGC TCTATGATGT AACATGTGAA AAAAGCTTTC TTAATGTGCG AGAATGGGTA 
GATATGATTG AGGATGCAAC TCATGAGAAT ATTCCAATTA TGATGGTGGG AAACAAAGCA 
GATCTCCGTC AAGCTGTTAC AGAACAAGGG CAAAAATGTG TGCCTATAAA CTATGGAGAA 
AAGCTGGCTA TGACTTATAA TGCGCTGTTC TGTGAAACAA GTGCTAAGGA TGGATCTAAT 
ATTGTAGAAG CAGTTCTTCA TCTTGCTCGT GAAGTGCGAA AACGAAGTGA TAATCAAGAT 
GATACAAGGT CAGTAACCAG CCTGGCTGGG ACACCTGTCA AAAAATCGGC ACTCACGAAA 
AACTGTTGCA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014791509.1
CDS	71..2248
Translation	MDPETAGDELSRLRALFVACDASGSGRIEREDFAALCAELRVRPAEAEAIFQRLDSDHDGAITFPEFARGFRGATRRRPGGGETEGDEKEEEEEEEEAAAAWTAAGIEQPWRDFEVRLGDEARYIPRQEQVSVLYQNIHIAEPRLIQPYEHVIKNFIREIKLQSTEMETLAIAVKRAQDKAAVQLSELEEEMEQRIQAAEHKVKKEEKRKAEEALNELKRQYDTEVGDLQVTIKKLKKLEEQSKNVNHREDVIELKKRMHDMLLENQKLKKDLLEAQTNIAFLQSELDSLKSEYADQTLNTERDLEMIREYTEDRDNLERQIEILQSANRKLHDSNDGLRSALENSLTKYNRSLRLANTSPGSTISRSSPKFNGGQSPPNPRYDRSSHSSCVDEDYDSLALCDPMQRINCEVDSLPESCFDSGLSTLRDSNEYDSEVEYRHQRIFQRSQCMQESFGGDASDTDVPEIRDEEAYSPDNSSTVLDWKPSRPVSRSSSVASTKKYISALTPQSDAGECAQKYPSSEKAYKIVLAGDAAVGKSSFLMRLCKNEFRGNTSATLGVDFQMKRLIVDGEPTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWVDMIEDATHENIPIMMVGNKADLRQAVTEQGQKCVPINYGEKLAMTYNALFCETSAKDGSNIVEAVLHLAREVRKRSDNQDDTRSVTSLAGTPVKKSALTKNCCNV

Target ORF information:

RefSeq Version	XM_014936023.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax RAS and EF-hand domain containing (RASEF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936023.1

ORF Insert Sequence:

ATGGATCCCG AGACGGCGGG GGACGAGCTG TCCCGCCTGC GCGCCCTCTT CGTGGCCTGC 
GACGCCAGCG GCTCGGGCCG CATCGAGCGG GAGGACTTCG CGGCGCTCTG CGCCGAGCTG 
CGGGTGCGGC CGGCCGAGGC CGAGGCGATC TTCCAGCGCC TCGACAGCGA CCACGACGGG 
GCCATCACCT TCCCGGAGTT TGCCCGCGGC TTCCGCGGCG CCACCCGACG GCGGCCCGGC 
GGCGGCGAGA CGGAGGGGGA CGAGAAGGAG GAGGAGGAGG AGGAGGAGGA GGCGGCGGCG 
GCGTGGACCG CCGCGGGGAT AGAGCAGCCC TGGCGGGACT TCGAGGTGCG GCTCGGGGAC 
GAGGCGAGGT ACATCCCCAG ACAAGAGCAG GTCAGTGTTC TGTATCAGAA CATACACATA 
GCGGAACCAA GATTAATCCA GCCGTACGAA CATGTCATTA AGAACTTCAT CCGAGAGATC 
AAGCTTCAAA GCACAGAGAT GGAAACTTTG GCCATTGCCG TAAAAAGAGC TCAGGATAAA 
GCGGCAGTTC AGCTCAGTGA GCTGGAGGAA GAGATGGAAC AACGGATACA GGCTGCAGAG 
CACAAAGTTA AAAAGGAAGA GAAGCGAAAA GCTGAAGAAG CACTAAATGA GCTAAAGCGC 
CAGTATGATA CTGAAGTTGG GGATCTTCAA GTGACAATAA AAAAACTTAA AAAGTTGGAG 
GAGCAATCCA AAAACGTAAA TCACAGGGAA GATGTGATTG AATTGAAAAA AAGAATGCAT 
GATATGTTGC TGGAAAATCA GAAACTTAAG AAAGATCTTT TAGAAGCACA AACAAATATA 
GCTTTTCTAC AGAGTGAATT AGATAGCTTG AAAAGCGAGT ATGCAGATCA GACTTTGAAC 
ACGGAGAGAG ACCTAGAAAT GATTCGAGAA TATACTGAAG ACAGAGATAA TCTGGAACGA 
CAAATTGAAA TACTTCAATC AGCTAATAGG AAGCTACATG ATAGCAACGA TGGTTTAAGA 
AGTGCGCTTG AAAACAGTTT GACCAAGTAC AACAGATCAT TGAGGTTAGC AAACACCTCA 
CCAGGAAGTA CCATTTCTAG AAGCAGTCCT AAATTTAATG GTGGACAGTC TCCTCCAAAC 
CCACGGTATG ATAGATCATC TCATTCCTCT TGTGTGGATG AAGATTATGA TTCTTTAGCC 
CTTTGTGATC CTATGCAAAG GATAAACTGT GAAGTTGACA GCTTACCTGA GAGCTGTTTT 
GACAGTGGTT TGTCTACCCT GAGAGATTCA AATGAGTATG ATTCAGAAGT GGAATACAGG 
CATCAAAGGA TTTTTCAAAG GTCACAGTGT ATGCAAGAAA GCTTTGGTGG TGATGCTTCT 
GATACAGATG TTCCGGAGAT CAGGGATGAA GAGGCATACA GTCCTGATAA CAGCAGTACA 
GTATTAGACT GGAAGCCCTC ACGGCCAGTA AGTCGAAGCA GCTCAGTTGC TTCAACGAAG 
AAATACATAT CTGCTCTCAC CCCTCAGTCA GATGCGGGCG AATGTGCTCA GAAATACCCC 
AGCTCGGAGA AGGCCTACAA GATTGTTCTC GCCGGAGACG CAGCAGTGGG AAAGTCCAGT 
TTCCTTATGA GACTTTGCAA GAATGAATTT CGAGGAAACA CCAGTGCAAC CCTGGGTGTG 
GATTTTCAAA TGAAAAGACT AATTGTTGAT GGAGAACCTA CAGTGCTACA ACTGTGGGAC 
ACAGCTGGGC AGGAGAGATT CCGGAGTATT GCTAAATCAT ACTTCAGAAG AGCGGATGGC 
GTCTTACTGC TCTATGATGT AACATGTGAA AAAAGCTTTC TTAATGTGCG AGAATGGGTA 
GATATGATTG AGGATGCAAC TCATGAGAAT ATTCCAATTA TGATGGTGGG AAACAAAGCA 
GATCTCCGTC AAGCTGTTAC AGAACAAGGG CAAAAATGTG TGCCTATAAA CTATGGAGAA 
AAGCTGGCTA TGACTTATAA TGCGCTGTTC TGTGAAACAA GTGCTAAGGA TGGATCTAAT 
ATTGTAGAAG CAGTTCTTCA TCTTGCTCGT GAAGTGCGAA AACGAAGTGA TAATCAAGAT 
GATACAAGGT CAGTAACCAG CCTGGCTGGG ACACCTGTCA AAAAATCGGC ACTCACGAAA 
AACTGTTGCA ATGTTTAA