TMEFF1 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEFF1
Entrez Gene ID	106885916
Full Name	transmembrane protein with EGF like and two follistatin like domains 1
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014938439.1	XP_014793925.1	tomoregulin-1

Pathways from BioSystems
Gene Ontology

The following TMEFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa62455	XM_014938439.1 Latest version!	Calidris pugnax transmembrane protein with EGF-like and two follistatin-like domains 1 (TMEFF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEFF1 ( XM_014938439.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014793925.1 Calidris pugnax transmembrane protein with EGF-like and two follistatin-like domains 1 (TMEFF1), mRNA.

Price & Availability↑

CloneID	OCa62455
Clone ID Related Accession (Same CDS sequence)	XM_014938439.1
Accession Version	XM_014938439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	tomoregulin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 43% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGCAG GGCCAGGGCG CCAGGGAGAG GGCTGGAAGC TGGGTCCTGT AGGGCTCCTC 
CGCCTCAGCA AATCCCAACA ATGGCGGAAA GAAGATGCCT GTGATGCCAG GCCATGCATG 
TGCCTGGAAA ACGTCATCGA TGTGGCCGAA GAATCCATGT TAAGCCGAGC TTTGATGTCT 
TTGAAAACCA AGGCCCGTTT CCCCGCTTAC AGGCACCTGT GGCAGAGGAA AGCTGCCCTT 
TTCAGAGAAA ATGAACTCAT CACAAAAGCA GTTGATGGCG AGTACTGGAT GGGATCCTCC 
CCTAGGAAAT GCTTGTTAAT CACAGCCAGC CAGTTTTCCG AGCAGCGCAC TGGTGCTGGA 
GCACTGGTTG ACTGGGAACA CGGCTGTGGC CAGTGCTACC CAGGAGAGCA GCTTGGCAGG 
TTGCGTGTCT TCGCGCCTTT TGAACTAGGA GACAATGCGG TGTGCCTTGC AGAGGAGAAA 
GCTTGGCAGA GCTGCTGCGA GGTGGACAGT CAGTGCCCGT TCATTGGCAA AGCTGGCACA 
GCCAGAGAAT CGGCTGCGGC CATACGCAAG CAGTTTAATA TGAGGAAAGT TGCAGACAGG 
TTTGACAGCC CAGACTCGGA AGTAGTGCCA TATGTAGATA TGACAATATC ATTTAGTGCT 
ATCAGGCCGA TCTGTAGGCT GCAGTTTTGT GATATTGTCA CATTTTGGTT GAGGGCAGCA 
GAACTAAATG TGAGGGAATC CGACGTAAAA GTGTGTGATG AGTCGTCATG TAAATACGGA 
GGAGTGTGCA AGGAAGAGGG CGACGGCTTG AAATGTGCGT GTCAGTTCCA GTGTCACACA 
AACTATATTC CTGTTTGTGG ATCAAATGGA GACACTTACC AAAACGAATG CTTCCTCAGG 
AGAGCTTCTT GCAAACATCA AAAAGAGATA ACAGTGGTAT CAAGAGGACC TTGTTATTCT 
GATAATGGCT CTGGGTCAGG AGAAGGAGAG TATGAAGGAT CTGGGACAGA AGTTCAGAGA 
AAACACTCAA AATGTGGAGT TTGCAAATAT AGGGCTGAAT GTGATGAAGA TGCAGAAAAC 
GTTGGGTGTG TATGTAATAT AGACTGCAGT GGATACAGTT TTAATCCTGT ATGTGCTTCT 
GATGGAAGTT CCTATAACAA TCCATGCTTT GTTAGAGAAG CATCATGTCT GAGGCAAGAG 
CAGATCGACA TCAGGCATCT TGGACACTGC TCAGACACAG ATGACGCAAA CGCAGTTGGA 
AAGAAAGATG ATGGCATGCA GTATCGGCCG GAGGTGAAAG ATGCCAGTGA TCAAAGAGAA 
GACATTTACA TTGGAAACCA CATACCTTGT TCAGAAAGCT TCAACGGCTA CTGTATACAT 
GGAAAATGTG AATTCATTTA TTCCACTCAG AAGGCTTCCT GCCGGTGTGA ATCAGGATAT 
ACTGGACAGC ACTGTGAAAA GACAGACTTT AGTATTCTTT ATGTTGTCCC AAGTAGACAA 
AAGCTTACGC ATGTCCTTAT TGCAGCAATA ATTGGAGCTG TACAGATTGC CATTATAGTT 
GCAATTGTCA TGTGCATAAC AAGAAAATGC CCCAAAAACA ATAGAGGACG TCGGCAGAAG 
CAAAACCTAG GCCATTTTAC TTCAGATACA TCATCCAGAA TGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014793925.1
CDS	1..1668
Translation	MAAGPGRQGEGWKLGPVGLLRLSKSQQWRKEDACDARPCMCLENVIDVAEESMLSRALMSLKTKARFPAYRHLWQRKAALFRENELITKAVDGEYWMGSSPRKCLLITASQFSEQRTGAGALVDWEHGCGQCYPGEQLGRLRVFAPFELGDNAVCLAEEKAWQSCCEVDSQCPFIGKAGTARESAAAIRKQFNMRKVADRFDSPDSEVVPYVDMTISFSAIRPICRLQFCDIVTFWLRAAELNVRESDVKVCDESSCKYGGVCKEEGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRASCKHQKEITVVSRGPCYSDNGSGSGEGEYEGSGTEVQRKHSKCGVCKYRAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCLRQEQIDIRHLGHCSDTDDANAVGKKDDGMQYRPEVKDASDQREDIYIGNHIPCSESFNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	XM_014938439.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax transmembrane protein with EGF-like and two follistatin-like domains 1 (TMEFF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014938439.1

ORF Insert Sequence:

ATGGCGGCAG GGCCAGGGCG CCAGGGAGAG GGCTGGAAGC TGGGTCCTGT AGGGCTCCTC 
CGCCTCAGCA AATCCCAACA ATGGCGGAAA GAAGATGCCT GTGATGCCAG GCCATGCATG 
TGCCTGGAAA ACGTCATCGA TGTGGCCGAA GAATCCATGT TAAGCCGAGC TTTGATGTCT 
TTGAAAACCA AGGCCCGTTT CCCCGCTTAC AGGCACCTGT GGCAGAGGAA AGCTGCCCTT 
TTCAGAGAAA ATGAACTCAT CACAAAAGCA GTTGATGGCG AGTACTGGAT GGGATCCTCC 
CCTAGGAAAT GCTTGTTAAT CACAGCCAGC CAGTTTTCCG AGCAGCGCAC TGGTGCTGGA 
GCACTGGTTG ACTGGGAACA CGGCTGTGGC CAGTGCTACC CAGGAGAGCA GCTTGGCAGG 
TTGCGTGTCT TCGCGCCTTT TGAACTAGGA GACAATGCGG TGTGCCTTGC AGAGGAGAAA 
GCTTGGCAGA GCTGCTGCGA GGTGGACAGT CAGTGCCCGT TCATTGGCAA AGCTGGCACA 
GCCAGAGAAT CGGCTGCGGC CATACGCAAG CAGTTTAATA TGAGGAAAGT TGCAGACAGG 
TTTGACAGCC CAGACTCGGA AGTAGTGCCA TATGTAGATA TGACAATATC ATTTAGTGCT 
ATCAGGCCGA TCTGTAGGCT GCAGTTTTGT GATATTGTCA CATTTTGGTT GAGGGCAGCA 
GAACTAAATG TGAGGGAATC CGACGTAAAA GTGTGTGATG AGTCGTCATG TAAATACGGA 
GGAGTGTGCA AGGAAGAGGG CGACGGCTTG AAATGTGCGT GTCAGTTCCA GTGTCACACA 
AACTATATTC CTGTTTGTGG ATCAAATGGA GACACTTACC AAAACGAATG CTTCCTCAGG 
AGAGCTTCTT GCAAACATCA AAAAGAGATA ACAGTGGTAT CAAGAGGACC TTGTTATTCT 
GATAATGGCT CTGGGTCAGG AGAAGGAGAG TATGAAGGAT CTGGGACAGA AGTTCAGAGA 
AAACACTCAA AATGTGGAGT TTGCAAATAT AGGGCTGAAT GTGATGAAGA TGCAGAAAAC 
GTTGGGTGTG TATGTAATAT AGACTGCAGT GGATACAGTT TTAATCCTGT ATGTGCTTCT 
GATGGAAGTT CCTATAACAA TCCATGCTTT GTTAGAGAAG CATCATGTCT GAGGCAAGAG 
CAGATCGACA TCAGGCATCT TGGACACTGC TCAGACACAG ATGACGCAAA CGCAGTTGGA 
AAGAAAGATG ATGGCATGCA GTATCGGCCG GAGGTGAAAG ATGCCAGTGA TCAAAGAGAA 
GACATTTACA TTGGAAACCA CATACCTTGT TCAGAAAGCT TCAACGGCTA CTGTATACAT 
GGAAAATGTG AATTCATTTA TTCCACTCAG AAGGCTTCCT GCCGGTGTGA ATCAGGATAT 
ACTGGACAGC ACTGTGAAAA GACAGACTTT AGTATTCTTT ATGTTGTCCC AAGTAGACAA 
AAGCTTACGC ATGTCCTTAT TGCAGCAATA ATTGGAGCTG TACAGATTGC CATTATAGTT 
GCAATTGTCA TGTGCATAAC AAGAAAATGC CCCAAAAACA ATAGAGGACG TCGGCAGAAG 
CAAAACCTAG GCCATTTTAC TTCAGATACA TCATCCAGAA TGGTTTAA