GIGYF2 gene cDNA ORF clone, Calidris pugnax(ruff)

mRNA	Protein	Name
XM_014952133.1	XP_014807619.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
XM_014952138.1	XP_014807624.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X5
XM_014952131.1	XP_014807617.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
XM_014952132.1	XP_014807618.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
XM_014952140.1	XP_014807626.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X7
XM_014952141.1	XP_014807627.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X8
XM_014952136.1	XP_014807622.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X3
XM_014952137.1	XP_014807623.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X4
XM_014952139.1	XP_014807625.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X6
XM_014952130.1	XP_014807616.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
XM_014952135.1	XP_014807621.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa72425	XM_014952131.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72425	XM_014952132.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72425	XM_014952130.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72425	XM_014952133.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72426	XM_014952135.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72427	XM_014952136.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72428	XM_014952137.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72429	XM_014952138.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72430	XM_014952139.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72431	XM_014952140.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCa72432	XM_014952141.1 Latest version!	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

GIGYF2 ( XM_014952133.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807619.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa72425
Clone ID Related Accession (Same CDS sequence)	XM_014952133.1 , XM_014952131.1 , XM_014952132.1 , XM_014952130.1
Accession Version	XM_014952133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG ATGACAGTGA GAGCTGAGAG GGGTGAAGCA 
GTAGACTTAA TCTGCCTGGA CTTCAGGAAG GCTTTTGATG TAATACTGCA TAAAAGACTA 
GTATATAAGG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807619.1
CDS	150..4088
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEMTVRAERGEAVDLICLDFRKAFDVILHKRLVYKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952133.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952133.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG ATGACAGTGA GAGCTGAGAG GGGTGAAGCA 
GTAGACTTAA TCTGCCTGGA CTTCAGGAAG GCTTTTGATG TAATACTGCA TAAAAGACTA 
GTATATAAGG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952131.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807617.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa72425
Clone ID Related Accession (Same CDS sequence)	XM_014952133.1 , XM_014952131.1 , XM_014952132.1 , XM_014952130.1
Accession Version	XM_014952131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG ATGACAGTGA GAGCTGAGAG GGGTGAAGCA 
GTAGACTTAA TCTGCCTGGA CTTCAGGAAG GCTTTTGATG TAATACTGCA TAAAAGACTA 
GTATATAAGG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807617.1
CDS	1074..5012
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEMTVRAERGEAVDLICLDFRKAFDVILHKRLVYKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952131.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952131.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG ATGACAGTGA GAGCTGAGAG GGGTGAAGCA 
GTAGACTTAA TCTGCCTGGA CTTCAGGAAG GCTTTTGATG TAATACTGCA TAAAAGACTA 
GTATATAAGG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952132.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807618.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA.

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CloneID	OCa72425
Clone ID Related Accession (Same CDS sequence)	XM_014952133.1 , XM_014952131.1 , XM_014952132.1 , XM_014952130.1
Accession Version	XM_014952132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG ATGACAGTGA GAGCTGAGAG GGGTGAAGCA 
GTAGACTTAA TCTGCCTGGA CTTCAGGAAG GCTTTTGATG TAATACTGCA TAAAAGACTA 
GTATATAAGG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807618.1
CDS	135..4073
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEMTVRAERGEAVDLICLDFRKAFDVILHKRLVYKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952132.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952132.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG ATGACAGTGA GAGCTGAGAG GGGTGAAGCA 
GTAGACTTAA TCTGCCTGGA CTTCAGGAAG GCTTTTGATG TAATACTGCA TAAAAGACTA 
GTATATAAGG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952130.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807616.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa72425
Clone ID Related Accession (Same CDS sequence)	XM_014952133.1 , XM_014952131.1 , XM_014952132.1 , XM_014952130.1
Accession Version	XM_014952130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG ATGACAGTGA GAGCTGAGAG GGGTGAAGCA 
GTAGACTTAA TCTGCCTGGA CTTCAGGAAG GCTTTTGATG TAATACTGCA TAAAAGACTA 
GTATATAAGG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807616.1
CDS	125..4063
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEMTVRAERGEAVDLICLDFRKAFDVILHKRLVYKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952130.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952130.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG ATGACAGTGA GAGCTGAGAG GGGTGAAGCA 
GTAGACTTAA TCTGCCTGGA CTTCAGGAAG GCTTTTGATG TAATACTGCA TAAAAGACTA 
GTATATAAGG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952135.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807621.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCa72426
Clone ID Related Accession (Same CDS sequence)	XM_014952135.1
Accession Version	XM_014952135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAATG ACAGTGAGAG CTGAGAGGGG TGAAGCAGTA 
GACTTAATCT GCCTGGACTT CAGGAAGGCT TTTGATGTAA TACTGCATAA AAGACTAGTA 
TATAAGGAGG AAGCCCTGAG GCGACAACGA GAGCAGGAAA TTGCACTAAG GCGGCAGCGG 
GAGGAAGAGG AGCGGCAACA GCAGGAGGAA GCACTTAGAA GACTGGAAGA GAGAAGAAGA 
GAAGAGGAGG AAAGACGGCA GCGAGAGGAG TTAATTCGTA AACAGGAAGA GGAGGCTGCC 
AAGTGGGCTC GTGAAGAGGA GGAAGCTCAA CGGCGACTTG AAGAAAGTCG GCTACGCATG 
GAAGAAGAGG CAGCCAGACA GCGGCTCGAA GAAGAAGAGC GAAAGCGCAA GGAGCTGGAA 
CTCCAGCGCC AGAAAGAAAT AATGAGACAG AGGCAGCAGC AGCAGGAGGC TTTACGACGG 
CTGCAACAAC AGCAGCAACA GCAGCAACTT GCACAAATGA AGCTCCCTTC ATCTTCGACA 
TGGGGTCAGC AATCAAATTC AGGAGCTTGT CAATCCCAGA CCACACTGTC ATTGGCAGAA 
ATCCAAAAGT TAGAAGAAGA ACGAGAACGG CAGCTTCGAG AAGAGCAAAG GCGTCAGCAG 
CGTGAACTAA TGAAGGCCCT CCAGCAACAG CAGCAGCAAC AGCAGCAAAA ATTGTCAGGC 
TGGGGTAATG TCACCAAACC AACAGGCACC ACCAAGTCCC TGCTGGAGAT ACAGCAGGAG 
GAGGCAAGGC AGATGCAGAA ACAGCAGCAA CAGCAGCACC AGCAGCCGAA CAGAGTCAGA 
AATACTACGC ACTCTAACCT GCACACCAGC ATTGGGAATT CAGTGTGGGG CTCCCTAAAC 
ACTAATCCCA GCAACCAGTG GGCATCTGAC CTAGTCAGTA GCATCTGGAG TAATGCTGAT 
ACCAAAAACT CAAACATGGG TTTCTGGGAT GATGCAGTGA AGGAAGTGGG ACCTCGTAAC 
TCGACCAATA AAAACAAAAG CAATGCCAGC CTCAGTAAAT CTGTGGGTCT TACAAGTAGA 
CAAAACAAGA AGGTGGAAGA AGAGGAGAAG CTGTTGAAAT TGTTCCAGGG GGTTAATAAA 
GCCCAGGATG GATTCACTCA GTGGTGTGAA CAGATGCTTC ATGCACTCAA CACAGCCAAC 
AACTTAGATG TTCCCACGTT TGTTTCTTTC CTGAAGGAAG TGGAGTCTCC CTACGAGGTC 
CATGATTACA TCAGAGCCTA TCTTGGAGAT ACTCCTGAGG CCAAGGAGTT TGCCAAGCAG 
TTTCTCGAGC GCCGTGCCAA ACAGAAAGCC AGTCAGCAGC GACAACAGCA GCAGCAACAG 
GATTCAGTGT GGGGGATGAA CCACAGTTCG CTACATTCGG TCTTTCAGAC CAACCAGAGC 
AACAACCAGC AATCCAACTT TGAGGCTGTG CAGAGCGGGA AGAAGAAGAA GAAACAGAAA 
ATGGTTCGCG CAGATCCCAG CTTGCTAGGG TTTTCAGTGA ATGCCTCGTC AGAGCGGCTC 
AATATGGGAG AAATAGAGAC TTTGGAAGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807621.1
CDS	125..4060
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQMTVRAERGEAVDLICLDFRKAFDVILHKRLVYKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952135.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952135.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAATG ACAGTGAGAG CTGAGAGGGG TGAAGCAGTA 
GACTTAATCT GCCTGGACTT CAGGAAGGCT TTTGATGTAA TACTGCATAA AAGACTAGTA 
TATAAGGAGG AAGCCCTGAG GCGACAACGA GAGCAGGAAA TTGCACTAAG GCGGCAGCGG 
GAGGAAGAGG AGCGGCAACA GCAGGAGGAA GCACTTAGAA GACTGGAAGA GAGAAGAAGA 
GAAGAGGAGG AAAGACGGCA GCGAGAGGAG TTAATTCGTA AACAGGAAGA GGAGGCTGCC 
AAGTGGGCTC GTGAAGAGGA GGAAGCTCAA CGGCGACTTG AAGAAAGTCG GCTACGCATG 
GAAGAAGAGG CAGCCAGACA GCGGCTCGAA GAAGAAGAGC GAAAGCGCAA GGAGCTGGAA 
CTCCAGCGCC AGAAAGAAAT AATGAGACAG AGGCAGCAGC AGCAGGAGGC TTTACGACGG 
CTGCAACAAC AGCAGCAACA GCAGCAACTT GCACAAATGA AGCTCCCTTC ATCTTCGACA 
TGGGGTCAGC AATCAAATTC AGGAGCTTGT CAATCCCAGA CCACACTGTC ATTGGCAGAA 
ATCCAAAAGT TAGAAGAAGA ACGAGAACGG CAGCTTCGAG AAGAGCAAAG GCGTCAGCAG 
CGTGAACTAA TGAAGGCCCT CCAGCAACAG CAGCAGCAAC AGCAGCAAAA ATTGTCAGGC 
TGGGGTAATG TCACCAAACC AACAGGCACC ACCAAGTCCC TGCTGGAGAT ACAGCAGGAG 
GAGGCAAGGC AGATGCAGAA ACAGCAGCAA CAGCAGCACC AGCAGCCGAA CAGAGTCAGA 
AATACTACGC ACTCTAACCT GCACACCAGC ATTGGGAATT CAGTGTGGGG CTCCCTAAAC 
ACTAATCCCA GCAACCAGTG GGCATCTGAC CTAGTCAGTA GCATCTGGAG TAATGCTGAT 
ACCAAAAACT CAAACATGGG TTTCTGGGAT GATGCAGTGA AGGAAGTGGG ACCTCGTAAC 
TCGACCAATA AAAACAAAAG CAATGCCAGC CTCAGTAAAT CTGTGGGTCT TACAAGTAGA 
CAAAACAAGA AGGTGGAAGA AGAGGAGAAG CTGTTGAAAT TGTTCCAGGG GGTTAATAAA 
GCCCAGGATG GATTCACTCA GTGGTGTGAA CAGATGCTTC ATGCACTCAA CACAGCCAAC 
AACTTAGATG TTCCCACGTT TGTTTCTTTC CTGAAGGAAG TGGAGTCTCC CTACGAGGTC 
CATGATTACA TCAGAGCCTA TCTTGGAGAT ACTCCTGAGG CCAAGGAGTT TGCCAAGCAG 
TTTCTCGAGC GCCGTGCCAA ACAGAAAGCC AGTCAGCAGC GACAACAGCA GCAGCAACAG 
GATTCAGTGT GGGGGATGAA CCACAGTTCG CTACATTCGG TCTTTCAGAC CAACCAGAGC 
AACAACCAGC AATCCAACTT TGAGGCTGTG CAGAGCGGGA AGAAGAAGAA GAAACAGAAA 
ATGGTTCGCG CAGATCCCAG CTTGCTAGGG TTTTCAGTGA ATGCCTCGTC AGAGCGGCTC 
AATATGGGAG AAATAGAGAC TTTGGAAGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952136.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807622.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X6, mRNA.

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CloneID	OCa72427
Clone ID Related Accession (Same CDS sequence)	XM_014952136.1
Accession Version	XM_014952136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG CAGTGGAAGA AGCAGCCCAA ACATCTTCAC CGGCTACCAG GTCAGATACC 
CCACCGAAAT CCCAGCCTCC AGACCCGCTG CAGACGAGCC TCTTTGAAAG GAAGGAAGAG 
TCTGTGCCAG AAAGAACAGA AAAAACAGAA GACAAAGAGA ATCGAACAGA GAATACACCA 
CCAGCCAAAA TGTCCAACAG AGGGGAAGAT TTGGCTTCTG TTGCTCAACC TTTGCCACAG 
ATTTCTGCAG ACACAGCTTC TCCTGCTCAT CTTTCTCCTC CTGTTTCCAA TTCAAATCCT 
GCTCTGCGGC CAGTTCAAAC ACCTGTCACA GCTGCTCCAG GCATGGGCAA CATTCCCACT 
GACCCAGATG ATGAAGAGGG CCTCAAACAC CTAGAGCAGC AAGCAGAGAA GATGGTGGCA 
TACCTGCAGG ACAGTGCCCT TGATGATGAA AGGCTAGCAG CAAAAATTCA AGAGCACAGA 
GCAAAGGGTT CCTCTATGCC GCTGTTCCAT GAAGCCATGC AGAAATGGTA CTACAAAGAT 
CCACAAGGAG AAATTCAGGG TCCTTTCAGT AATCAGGAGA TGGCAGAGTG GTTCCAGGCC 
GGATACTTCA CCATGTCGCT GTTGGTCAAG AGAGCCTGCG ATGAGACTTT CCAGCCACTG 
GGAGACATCA TGAAAATGTG GGGCAGGGTA CCCTTCACTC CAGGTCCAGC ACCGCTTCCA 
CACCTGGGCG AGCTGGACCA AGAGCGGCTG ACTAGGCAAC AAGAGTTGGC CCTGATCCAA 
ATGCAGCACC TCCAGTATCA GCATCTTCTA ATACAACAAC AATATGCCCA GGTGCTGGCC 
CAGCAGCAAA AGGCAGCCCT CTCATCCCAG CAGCAGCAGC AGCTCGCTAT TCTCTTGCAA 
CAGTTCCAGG CCCTGAAGAT GAGAATATCT GAACAGAACA TGATGCCACC TGTTACAAGG 
TCTGTGTCAG TGCCGGACAC TGGCTCAATA TGGGAACTTC AGTCAGCTTC ACAGCCTACA 
GTCTGGGAAG GAAGCAGTGT CTGGGATCTC CCCATTGATA CTGCAGCTCA GGGACCAGCT 
CTAGAACAAC TTCAGCAGCT CGAGAAGGCC AAAGCTGCAA AGCTAGAGCA AGAGAGGAGA 
GAAGTGGAAA TGAGGGCCAA ACGAGAGGAA GAGGAGAGGA AAAGGCAGGA GGAGCTTCGG 
AGGCAACAAG AAGAGCTACT TAGGAGGCAG CAGGAAGATG AGAGGAAACG ACGGGAAGAA 
GAAGAACTGG CCCGCAGGAA GCAAGAGATG ACAGTGAGAG CTGAGAGGGG TGAAGCAGTA 
GACTTAATCT GCCTGGACTT CAGGAAGGCT TTTGATGTAA TACTGCATAA AAGACTAGTA 
TATAAGGAGG AAGCCCTGAG GCGACAACGA GAGCAGGAAA TTGCACTAAG GCGGCAGCGG 
GAGGAAGAGG AGCGGCAACA GCAGGAGGAA GCACTTAGAA GACTGGAAGA GAGAAGAAGA 
GAAGAGGAGG AAAGACGGCA GCGAGAGGAG TTAATTCGTA AACAGGAAGA GGAGGCTGCC 
AAGTGGGCTC GTGAAGAGGA GGAAGCTCAA CGGCGACTTG AAGAAAGTCG GCTACGCATG 
GAAGAAGAGG CAGCCAGACA GCGGCTCGAA GAAGAAGAGC GAAAGCGCAA GGAGCTGGAA 
CTCCAGCGCC AGAAAGAAAT AATGAGACAG AGGCAGCAGC AGCAGGAGGC TTTACGACGG 
CTGCAACAAC AGCAGCAACA GCAGCAACTT GCACAAATGA AGCTCCCTTC ATCTTCGACA 
TGGGGTCAGC AATCAAATTC AGGAGCTTGT CAATCCCAGA CCACACTGTC ATTGGCAGAA 
ATCCAAAAGT TAGAAGAAGA ACGAGAACGG CAGCTTCGAG AAGAGCAAAG GCGTCAGCAG 
CGTGAACTAA TGAAGGCCCT CCAGCAACAG CAGCAGCAAC AGCAGCAAAA ATTGTCAGGC 
TGGGGTAATG TCACCAAACC AACAGGCACC ACCAAGTCCC TGCTGGAGAT ACAGCAGGAG 
GAGGCAAGGC AGATGCAGAA ACAGCAGCAA CAGCAGCACC AGCAGCCGAA CAGAGTCAGA 
AATACTACGC ACTCTAACCT GCACACCAGC ATTGGGAATT CAGTGTGGGG CTCCCTAAAC 
ACTAATCCCA GCAACCAGTG GGCATCTGAC CTAGTCAGTA GCATCTGGAG TAATGCTGAT 
ACCAAAAACT CAAACATGGG TTTCTGGGAT GATGCAGTGA AGGAAGTGGG ACCTCGTAAC 
TCGACCAATA AAAACAAAAG CAATGCCAGC CTCAGTAAAT CTGTGGGTCT TACAAGTAGA 
CAAAACAAGA AGGTGGAAGA AGAGGAGAAG CTGTTGAAAT TGTTCCAGGG GGTTAATAAA 
GCCCAGGATG GATTCACTCA GTGGTGTGAA CAGATGCTTC ATGCACTCAA CACAGCCAAC 
AACTTAGATG TTCCCACGTT TGTTTCTTTC CTGAAGGAAG TGGAGTCTCC CTACGAGGTC 
CATGATTACA TCAGAGCCTA TCTTGGAGAT ACTCCTGAGG CCAAGGAGTT TGCCAAGCAG 
TTTCTCGAGC GCCGTGCCAA ACAGAAAGCC AGTCAGCAGC GACAACAGCA GCAGCAACAG 
GATTCAGTGT GGGGGATGAA CCACAGTTCG CTACATTCGG TCTTTCAGAC CAACCAGAGC 
AACAACCAGC AATCCAACTT TGAGGCTGTG CAGAGCGGGA AGAAGAAGAA GAAACAGAAA 
ATGGTTCGCG CAGATCCCAG CTTGCTAGGG TTTTCAGTGA ATGCCTCGTC AGAGCGGCTC 
AATATGGGAG AAATAGAGAC TTTGGAAGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807622.1
CDS	125..4060
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAAVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEMTVRAERGEAVDLICLDFRKAFDVILHKRLVYKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952136.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952136.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG CAGTGGAAGA AGCAGCCCAA ACATCTTCAC CGGCTACCAG GTCAGATACC 
CCACCGAAAT CCCAGCCTCC AGACCCGCTG CAGACGAGCC TCTTTGAAAG GAAGGAAGAG 
TCTGTGCCAG AAAGAACAGA AAAAACAGAA GACAAAGAGA ATCGAACAGA GAATACACCA 
CCAGCCAAAA TGTCCAACAG AGGGGAAGAT TTGGCTTCTG TTGCTCAACC TTTGCCACAG 
ATTTCTGCAG ACACAGCTTC TCCTGCTCAT CTTTCTCCTC CTGTTTCCAA TTCAAATCCT 
GCTCTGCGGC CAGTTCAAAC ACCTGTCACA GCTGCTCCAG GCATGGGCAA CATTCCCACT 
GACCCAGATG ATGAAGAGGG CCTCAAACAC CTAGAGCAGC AAGCAGAGAA GATGGTGGCA 
TACCTGCAGG ACAGTGCCCT TGATGATGAA AGGCTAGCAG CAAAAATTCA AGAGCACAGA 
GCAAAGGGTT CCTCTATGCC GCTGTTCCAT GAAGCCATGC AGAAATGGTA CTACAAAGAT 
CCACAAGGAG AAATTCAGGG TCCTTTCAGT AATCAGGAGA TGGCAGAGTG GTTCCAGGCC 
GGATACTTCA CCATGTCGCT GTTGGTCAAG AGAGCCTGCG ATGAGACTTT CCAGCCACTG 
GGAGACATCA TGAAAATGTG GGGCAGGGTA CCCTTCACTC CAGGTCCAGC ACCGCTTCCA 
CACCTGGGCG AGCTGGACCA AGAGCGGCTG ACTAGGCAAC AAGAGTTGGC CCTGATCCAA 
ATGCAGCACC TCCAGTATCA GCATCTTCTA ATACAACAAC AATATGCCCA GGTGCTGGCC 
CAGCAGCAAA AGGCAGCCCT CTCATCCCAG CAGCAGCAGC AGCTCGCTAT TCTCTTGCAA 
CAGTTCCAGG CCCTGAAGAT GAGAATATCT GAACAGAACA TGATGCCACC TGTTACAAGG 
TCTGTGTCAG TGCCGGACAC TGGCTCAATA TGGGAACTTC AGTCAGCTTC ACAGCCTACA 
GTCTGGGAAG GAAGCAGTGT CTGGGATCTC CCCATTGATA CTGCAGCTCA GGGACCAGCT 
CTAGAACAAC TTCAGCAGCT CGAGAAGGCC AAAGCTGCAA AGCTAGAGCA AGAGAGGAGA 
GAAGTGGAAA TGAGGGCCAA ACGAGAGGAA GAGGAGAGGA AAAGGCAGGA GGAGCTTCGG 
AGGCAACAAG AAGAGCTACT TAGGAGGCAG CAGGAAGATG AGAGGAAACG ACGGGAAGAA 
GAAGAACTGG CCCGCAGGAA GCAAGAGATG ACAGTGAGAG CTGAGAGGGG TGAAGCAGTA 
GACTTAATCT GCCTGGACTT CAGGAAGGCT TTTGATGTAA TACTGCATAA AAGACTAGTA 
TATAAGGAGG AAGCCCTGAG GCGACAACGA GAGCAGGAAA TTGCACTAAG GCGGCAGCGG 
GAGGAAGAGG AGCGGCAACA GCAGGAGGAA GCACTTAGAA GACTGGAAGA GAGAAGAAGA 
GAAGAGGAGG AAAGACGGCA GCGAGAGGAG TTAATTCGTA AACAGGAAGA GGAGGCTGCC 
AAGTGGGCTC GTGAAGAGGA GGAAGCTCAA CGGCGACTTG AAGAAAGTCG GCTACGCATG 
GAAGAAGAGG CAGCCAGACA GCGGCTCGAA GAAGAAGAGC GAAAGCGCAA GGAGCTGGAA 
CTCCAGCGCC AGAAAGAAAT AATGAGACAG AGGCAGCAGC AGCAGGAGGC TTTACGACGG 
CTGCAACAAC AGCAGCAACA GCAGCAACTT GCACAAATGA AGCTCCCTTC ATCTTCGACA 
TGGGGTCAGC AATCAAATTC AGGAGCTTGT CAATCCCAGA CCACACTGTC ATTGGCAGAA 
ATCCAAAAGT TAGAAGAAGA ACGAGAACGG CAGCTTCGAG AAGAGCAAAG GCGTCAGCAG 
CGTGAACTAA TGAAGGCCCT CCAGCAACAG CAGCAGCAAC AGCAGCAAAA ATTGTCAGGC 
TGGGGTAATG TCACCAAACC AACAGGCACC ACCAAGTCCC TGCTGGAGAT ACAGCAGGAG 
GAGGCAAGGC AGATGCAGAA ACAGCAGCAA CAGCAGCACC AGCAGCCGAA CAGAGTCAGA 
AATACTACGC ACTCTAACCT GCACACCAGC ATTGGGAATT CAGTGTGGGG CTCCCTAAAC 
ACTAATCCCA GCAACCAGTG GGCATCTGAC CTAGTCAGTA GCATCTGGAG TAATGCTGAT 
ACCAAAAACT CAAACATGGG TTTCTGGGAT GATGCAGTGA AGGAAGTGGG ACCTCGTAAC 
TCGACCAATA AAAACAAAAG CAATGCCAGC CTCAGTAAAT CTGTGGGTCT TACAAGTAGA 
CAAAACAAGA AGGTGGAAGA AGAGGAGAAG CTGTTGAAAT TGTTCCAGGG GGTTAATAAA 
GCCCAGGATG GATTCACTCA GTGGTGTGAA CAGATGCTTC ATGCACTCAA CACAGCCAAC 
AACTTAGATG TTCCCACGTT TGTTTCTTTC CTGAAGGAAG TGGAGTCTCC CTACGAGGTC 
CATGATTACA TCAGAGCCTA TCTTGGAGAT ACTCCTGAGG CCAAGGAGTT TGCCAAGCAG 
TTTCTCGAGC GCCGTGCCAA ACAGAAAGCC AGTCAGCAGC GACAACAGCA GCAGCAACAG 
GATTCAGTGT GGGGGATGAA CCACAGTTCG CTACATTCGG TCTTTCAGAC CAACCAGAGC 
AACAACCAGC AATCCAACTT TGAGGCTGTG CAGAGCGGGA AGAAGAAGAA GAAACAGAAA 
ATGGTTCGCG CAGATCCCAG CTTGCTAGGG TTTTCAGTGA ATGCCTCGTC AGAGCGGCTC 
AATATGGGAG AAATAGAGAC TTTGGAAGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952137.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807623.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCa72428
Clone ID Related Accession (Same CDS sequence)	XM_014952137.1
Accession Version	XM_014952137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGG CTGGCGAGAG 
CACATGGAGC GGCGGCGGAG GTTTGAGTTC GATTTCCGGG AACGAGACGA CGAGCGGGGC 
TATCGACGGG TACGGTGTGG CAGCGGCAGC ATGGACGACG ACAGAGACAG TCTCCCCGAG 
TGGTGCTTAG AAGATGCAGA AGAAGAAATG GGAACATTTG ACTCCTCCGG AGCGTTTCTC 
TCTTTAAAGA AGGTGCAGAA GGAGCCAATC CCTGAGGAAC AGGAGATGGA CTTCAGACCT 
GTGGAGGAAG GGGAAGAAAG ATCAGACTCT GAAGGGAGCC ACAACGAAGA AGCCAAAGAT 
CATGAAAAGA CCACCAAGAA AGAGGGGGAG AAAACAGAGA GAGTGGTGGC AGAAACAGTG 
GAAGAAGCAG CCCAAACATC TTCACCGGCT ACCAGGTCAG ATACCCCACC GAAATCCCAG 
CCTCCAGACC CGCTGCAGAC GAGCCTCTTT GAAAGGAAGG AAGAGTCTGT GCCAGAAAGA 
ACAGAAAAAA CAGAAGACAA AGAGAATCGA ACAGAGAATA CACCACCAGC CAAAATGTCC 
AACAGAGGGG AAGATTTGGC TTCTGTTGCT CAACCTTTGC CACAGATTTC TGCAGACACA 
GCTTCTCCTG CTCATCTTTC TCCTCCTGTT TCCAATTCAA ATCCTGCTCT GCGGCCAGTT 
CAAACACCTG TCACAGCTGC TCCAGGCATG GGCAACATTC CCACTGACCC AGATGATGAA 
GAGGGCCTCA AACACCTAGA GCAGCAAGCA GAGAAGATGG TGGCATACCT GCAGGACAGT 
GCCCTTGATG ATGAAAGGCT AGCAGCAAAA ATTCAAGAGC ACAGAGCAAA GGGTTCCTCT 
ATGCCGCTGT TCCATGAAGC CATGCAGAAA TGGTACTACA AAGATCCACA AGGAGAAATT 
CAGGGTCCTT TCAGTAATCA GGAGATGGCA GAGTGGTTCC AGGCCGGATA CTTCACCATG 
TCGCTGTTGG TCAAGAGAGC CTGCGATGAG ACTTTCCAGC CACTGGGAGA CATCATGAAA 
ATGTGGGGCA GGGTACCCTT CACTCCAGGT CCAGCACCGC TTCCACACCT GGGCGAGCTG 
GACCAAGAGC GGCTGACTAG GCAACAAGAG TTGGCCCTGA TCCAAATGCA GCACCTCCAG 
TATCAGCATC TTCTAATACA ACAACAATAT GCCCAGGTGC TGGCCCAGCA GCAAAAGGCA 
GCCCTCTCAT CCCAGCAGCA GCAGCAGCTC GCTATTCTCT TGCAACAGTT CCAGGCCCTG 
AAGATGAGAA TATCTGAACA GAACATGATG CCACCTGTTA CAAGGTCTGT GTCAGTGCCG 
GACACTGGCT CAATATGGGA ACTTCAGTCA GCTTCACAGC CTACAGTCTG GGAAGGAAGC 
AGTGTCTGGG ATCTCCCCAT TGATACTGCA GCTCAGGGAC CAGCTCTAGA ACAACTTCAG 
CAGCTCGAGA AGGCCAAAGC TGCAAAGCTA GAGCAAGAGA GGAGAGAAGT GGAAATGAGG 
GCCAAACGAG AGGAAGAGGA GAGGAAAAGG CAGGAGGAGC TTCGGAGGCA ACAAGAAGAG 
CTACTTAGGA GGCAGCAGGA AGATGAGAGG AAACGACGGG AAGAAGAAGA ACTGGCCCGC 
AGGAAGCAAG AGATGACAGT GAGAGCTGAG AGGGGTGAAG CAGTAGACTT AATCTGCCTG 
GACTTCAGGA AGGCTTTTGA TGTAATACTG CATAAAAGAC TAGTATATAA GGAGGAAGCC 
CTGAGGCGAC AACGAGAGCA GGAAATTGCA CTAAGGCGGC AGCGGGAGGA AGAGGAGCGG 
CAACAGCAGG AGGAAGCACT TAGAAGACTG GAAGAGAGAA GAAGAGAAGA GGAGGAAAGA 
CGGCAGCGAG AGGAGTTAAT TCGTAAACAG GAAGAGGAGG CTGCCAAGTG GGCTCGTGAA 
GAGGAGGAAG CTCAACGGCG ACTTGAAGAA AGTCGGCTAC GCATGGAAGA AGAGGCAGCC 
AGACAGCGGC TCGAAGAAGA AGAGCGAAAG CGCAAGGAGC TGGAACTCCA GCGCCAGAAA 
GAAATAATGA GACAGAGGCA GCAGCAGCAG GAGGCTTTAC GACGGCTGCA ACAACAGCAG 
CAACAGCAGC AACTTGCACA AATGAAGCTC CCTTCATCTT CGACATGGGG TCAGCAATCA 
AATTCAGGAG CTTGTCAATC CCAGACCACA CTGTCATTGG CAGAAATCCA AAAGTTAGAA 
GAAGAACGAG AACGGCAGCT TCGAGAAGAG CAAAGGCGTC AGCAGCGTGA ACTAATGAAG 
GCCCTCCAGC AACAGCAGCA GCAACAGCAG CAAAAATTGT CAGGCTGGGG TAATGTCACC 
AAACCAACAG GCACCACCAA GTCCCTGCTG GAGATACAGC AGGAGGAGGC AAGGCAGATG 
CAGAAACAGC AGCAACAGCA GCACCAGCAG CCGAACAGAG TCAGAAATAC TACGCACTCT 
AACCTGCACA CCAGCATTGG GAATTCAGTG TGGGGCTCCC TAAACACTAA TCCCAGCAAC 
CAGTGGGCAT CTGACCTAGT CAGTAGCATC TGGAGTAATG CTGATACCAA AAACTCAAAC 
ATGGGTTTCT GGGATGATGC AGTGAAGGAA GTGGGACCTC GTAACTCGAC CAATAAAAAC 
AAAAGCAATG CCAGCCTCAG TAAATCTGTG GGTCTTACAA GTAGACAAAA CAAGAAGGTG 
GAAGAAGAGG AGAAGCTGTT GAAATTGTTC CAGGGGGTTA ATAAAGCCCA GGATGGATTC 
ACTCAGTGGT GTGAACAGAT GCTTCATGCA CTCAACACAG CCAACAACTT AGATGTTCCC 
ACGTTTGTTT CTTTCCTGAA GGAAGTGGAG TCTCCCTACG AGGTCCATGA TTACATCAGA 
GCCTATCTTG GAGATACTCC TGAGGCCAAG GAGTTTGCCA AGCAGTTTCT CGAGCGCCGT 
GCCAAACAGA AAGCCAGTCA GCAGCGACAA CAGCAGCAGC AACAGGATTC AGTGTGGGGG 
ATGAACCACA GTTCGCTACA TTCGGTCTTT CAGACCAACC AGAGCAACAA CCAGCAATCC 
AACTTTGAGG CTGTGCAGAG CGGGAAGAAG AAGAAGAAAC AGAAAATGGT TCGCGCAGAT 
CCCAGCTTGC TAGGGTTTTC AGTGAATGCC TCGTCAGAGC GGCTCAATAT GGGAGAAATA 
GAGACTTTGG AAGACTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807623.1
CDS	124..4044
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEMTVRAERGEAVDLICLDFRKAFDVILHKRLVYKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952137.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952137.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGG CTGGCGAGAG 
CACATGGAGC GGCGGCGGAG GTTTGAGTTC GATTTCCGGG AACGAGACGA CGAGCGGGGC 
TATCGACGGG TACGGTGTGG CAGCGGCAGC ATGGACGACG ACAGAGACAG TCTCCCCGAG 
TGGTGCTTAG AAGATGCAGA AGAAGAAATG GGAACATTTG ACTCCTCCGG AGCGTTTCTC 
TCTTTAAAGA AGGTGCAGAA GGAGCCAATC CCTGAGGAAC AGGAGATGGA CTTCAGACCT 
GTGGAGGAAG GGGAAGAAAG ATCAGACTCT GAAGGGAGCC ACAACGAAGA AGCCAAAGAT 
CATGAAAAGA CCACCAAGAA AGAGGGGGAG AAAACAGAGA GAGTGGTGGC AGAAACAGTG 
GAAGAAGCAG CCCAAACATC TTCACCGGCT ACCAGGTCAG ATACCCCACC GAAATCCCAG 
CCTCCAGACC CGCTGCAGAC GAGCCTCTTT GAAAGGAAGG AAGAGTCTGT GCCAGAAAGA 
ACAGAAAAAA CAGAAGACAA AGAGAATCGA ACAGAGAATA CACCACCAGC CAAAATGTCC 
AACAGAGGGG AAGATTTGGC TTCTGTTGCT CAACCTTTGC CACAGATTTC TGCAGACACA 
GCTTCTCCTG CTCATCTTTC TCCTCCTGTT TCCAATTCAA ATCCTGCTCT GCGGCCAGTT 
CAAACACCTG TCACAGCTGC TCCAGGCATG GGCAACATTC CCACTGACCC AGATGATGAA 
GAGGGCCTCA AACACCTAGA GCAGCAAGCA GAGAAGATGG TGGCATACCT GCAGGACAGT 
GCCCTTGATG ATGAAAGGCT AGCAGCAAAA ATTCAAGAGC ACAGAGCAAA GGGTTCCTCT 
ATGCCGCTGT TCCATGAAGC CATGCAGAAA TGGTACTACA AAGATCCACA AGGAGAAATT 
CAGGGTCCTT TCAGTAATCA GGAGATGGCA GAGTGGTTCC AGGCCGGATA CTTCACCATG 
TCGCTGTTGG TCAAGAGAGC CTGCGATGAG ACTTTCCAGC CACTGGGAGA CATCATGAAA 
ATGTGGGGCA GGGTACCCTT CACTCCAGGT CCAGCACCGC TTCCACACCT GGGCGAGCTG 
GACCAAGAGC GGCTGACTAG GCAACAAGAG TTGGCCCTGA TCCAAATGCA GCACCTCCAG 
TATCAGCATC TTCTAATACA ACAACAATAT GCCCAGGTGC TGGCCCAGCA GCAAAAGGCA 
GCCCTCTCAT CCCAGCAGCA GCAGCAGCTC GCTATTCTCT TGCAACAGTT CCAGGCCCTG 
AAGATGAGAA TATCTGAACA GAACATGATG CCACCTGTTA CAAGGTCTGT GTCAGTGCCG 
GACACTGGCT CAATATGGGA ACTTCAGTCA GCTTCACAGC CTACAGTCTG GGAAGGAAGC 
AGTGTCTGGG ATCTCCCCAT TGATACTGCA GCTCAGGGAC CAGCTCTAGA ACAACTTCAG 
CAGCTCGAGA AGGCCAAAGC TGCAAAGCTA GAGCAAGAGA GGAGAGAAGT GGAAATGAGG 
GCCAAACGAG AGGAAGAGGA GAGGAAAAGG CAGGAGGAGC TTCGGAGGCA ACAAGAAGAG 
CTACTTAGGA GGCAGCAGGA AGATGAGAGG AAACGACGGG AAGAAGAAGA ACTGGCCCGC 
AGGAAGCAAG AGATGACAGT GAGAGCTGAG AGGGGTGAAG CAGTAGACTT AATCTGCCTG 
GACTTCAGGA AGGCTTTTGA TGTAATACTG CATAAAAGAC TAGTATATAA GGAGGAAGCC 
CTGAGGCGAC AACGAGAGCA GGAAATTGCA CTAAGGCGGC AGCGGGAGGA AGAGGAGCGG 
CAACAGCAGG AGGAAGCACT TAGAAGACTG GAAGAGAGAA GAAGAGAAGA GGAGGAAAGA 
CGGCAGCGAG AGGAGTTAAT TCGTAAACAG GAAGAGGAGG CTGCCAAGTG GGCTCGTGAA 
GAGGAGGAAG CTCAACGGCG ACTTGAAGAA AGTCGGCTAC GCATGGAAGA AGAGGCAGCC 
AGACAGCGGC TCGAAGAAGA AGAGCGAAAG CGCAAGGAGC TGGAACTCCA GCGCCAGAAA 
GAAATAATGA GACAGAGGCA GCAGCAGCAG GAGGCTTTAC GACGGCTGCA ACAACAGCAG 
CAACAGCAGC AACTTGCACA AATGAAGCTC CCTTCATCTT CGACATGGGG TCAGCAATCA 
AATTCAGGAG CTTGTCAATC CCAGACCACA CTGTCATTGG CAGAAATCCA AAAGTTAGAA 
GAAGAACGAG AACGGCAGCT TCGAGAAGAG CAAAGGCGTC AGCAGCGTGA ACTAATGAAG 
GCCCTCCAGC AACAGCAGCA GCAACAGCAG CAAAAATTGT CAGGCTGGGG TAATGTCACC 
AAACCAACAG GCACCACCAA GTCCCTGCTG GAGATACAGC AGGAGGAGGC AAGGCAGATG 
CAGAAACAGC AGCAACAGCA GCACCAGCAG CCGAACAGAG TCAGAAATAC TACGCACTCT 
AACCTGCACA CCAGCATTGG GAATTCAGTG TGGGGCTCCC TAAACACTAA TCCCAGCAAC 
CAGTGGGCAT CTGACCTAGT CAGTAGCATC TGGAGTAATG CTGATACCAA AAACTCAAAC 
ATGGGTTTCT GGGATGATGC AGTGAAGGAA GTGGGACCTC GTAACTCGAC CAATAAAAAC 
AAAAGCAATG CCAGCCTCAG TAAATCTGTG GGTCTTACAA GTAGACAAAA CAAGAAGGTG 
GAAGAAGAGG AGAAGCTGTT GAAATTGTTC CAGGGGGTTA ATAAAGCCCA GGATGGATTC 
ACTCAGTGGT GTGAACAGAT GCTTCATGCA CTCAACACAG CCAACAACTT AGATGTTCCC 
ACGTTTGTTT CTTTCCTGAA GGAAGTGGAG TCTCCCTACG AGGTCCATGA TTACATCAGA 
GCCTATCTTG GAGATACTCC TGAGGCCAAG GAGTTTGCCA AGCAGTTTCT CGAGCGCCGT 
GCCAAACAGA AAGCCAGTCA GCAGCGACAA CAGCAGCAGC AACAGGATTC AGTGTGGGGG 
ATGAACCACA GTTCGCTACA TTCGGTCTTT CAGACCAACC AGAGCAACAA CCAGCAATCC 
AACTTTGAGG CTGTGCAGAG CGGGAAGAAG AAGAAGAAAC AGAAAATGGT TCGCGCAGAT 
CCCAGCTTGC TAGGGTTTTC AGTGAATGCC TCGTCAGAGC GGCTCAATAT GGGAGAAATA 
GAGACTTTGG AAGACTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952138.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807624.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCa72429
Clone ID Related Accession (Same CDS sequence)	XM_014952138.1
Accession Version	XM_014952138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG GAAGCCCTGA GGCGACAACG AGAGCAGGAA 
ATTGCACTAA GGCGGCAGCG GGAGGAAGAG GAGCGGCAAC AGCAGGAGGA AGCACTTAGA 
AGACTGGAAG AGAGAAGAAG AGAAGAGGAG GAAAGACGGC AGCGAGAGGA GTTAATTCGT 
AAACAGGAAG AGGAGGCTGC CAAGTGGGCT CGTGAAGAGG AGGAAGCTCA ACGGCGACTT 
GAAGAAAGTC GGCTACGCAT GGAAGAAGAG GCAGCCAGAC AGCGGCTCGA AGAAGAAGAG 
CGAAAGCGCA AGGAGCTGGA ACTCCAGCGC CAGAAAGAAA TAATGAGACA GAGGCAGCAG 
CAGCAGGAGG CTTTACGACG GCTGCAACAA CAGCAGCAAC AGCAGCAACT TGCACAAATG 
AAGCTCCCTT CATCTTCGAC ATGGGGTCAG CAATCAAATT CAGGAGCTTG TCAATCCCAG 
ACCACACTGT CATTGGCAGA AATCCAAAAG TTAGAAGAAG AACGAGAACG GCAGCTTCGA 
GAAGAGCAAA GGCGTCAGCA GCGTGAACTA ATGAAGGCCC TCCAGCAACA GCAGCAGCAA 
CAGCAGCAAA AATTGTCAGG CTGGGGTAAT GTCACCAAAC CAACAGGCAC CACCAAGTCC 
CTGCTGGAGA TACAGCAGGA GGAGGCAAGG CAGATGCAGA AACAGCAGCA ACAGCAGCAC 
CAGCAGCCGA ACAGAGTCAG AAATACTACG CACTCTAACC TGCACACCAG CATTGGGAAT 
TCAGTGTGGG GCTCCCTAAA CACTAATCCC AGCAACCAGT GGGCATCTGA CCTAGTCAGT 
AGCATCTGGA GTAATGCTGA TACCAAAAAC TCAAACATGG GTTTCTGGGA TGATGCAGTG 
AAGGAAGTGG GACCTCGTAA CTCGACCAAT AAAAACAAAA GCAATGCCAG CCTCAGTAAA 
TCTGTGGGTC TTACAAGTAG ACAAAACAAG AAGGTGGAAG AAGAGGAGAA GCTGTTGAAA 
TTGTTCCAGG GGGTTAATAA AGCCCAGGAT GGATTCACTC AGTGGTGTGA ACAGATGCTT 
CATGCACTCA ACACAGCCAA CAACTTAGAT GTTCCCACGT TTGTTTCTTT CCTGAAGGAA 
GTGGAGTCTC CCTACGAGGT CCATGATTAC ATCAGAGCCT ATCTTGGAGA TACTCCTGAG 
GCCAAGGAGT TTGCCAAGCA GTTTCTCGAG CGCCGTGCCA AACAGAAAGC CAGTCAGCAG 
CGACAACAGC AGCAGCAACA GGATTCAGTG TGGGGGATGA ACCACAGTTC GCTACATTCG 
GTCTTTCAGA CCAACCAGAG CAACAACCAG CAATCCAACT TTGAGGCTGT GCAGAGCGGG 
AAGAAGAAGA AGAAACAGAA AATGGTTCGC GCAGATCCCA GCTTGCTAGG GTTTTCAGTG 
AATGCCTCGT CAGAGCGGCT CAATATGGGA GAAATAGAGA CTTTGGAAGA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807624.1
CDS	123..3959
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952138.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952138.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGCTAGA GCAAGAGAGG 
AGAGAAGTGG AAATGAGGGC CAAACGAGAG GAAGAGGAGA GGAAAAGGCA GGAGGAGCTT 
CGGAGGCAAC AAGAAGAGCT ACTTAGGAGG CAGCAGGAAG ATGAGAGGAA ACGACGGGAA 
GAAGAAGAAC TGGCCCGCAG GAAGCAAGAG GAAGCCCTGA GGCGACAACG AGAGCAGGAA 
ATTGCACTAA GGCGGCAGCG GGAGGAAGAG GAGCGGCAAC AGCAGGAGGA AGCACTTAGA 
AGACTGGAAG AGAGAAGAAG AGAAGAGGAG GAAAGACGGC AGCGAGAGGA GTTAATTCGT 
AAACAGGAAG AGGAGGCTGC CAAGTGGGCT CGTGAAGAGG AGGAAGCTCA ACGGCGACTT 
GAAGAAAGTC GGCTACGCAT GGAAGAAGAG GCAGCCAGAC AGCGGCTCGA AGAAGAAGAG 
CGAAAGCGCA AGGAGCTGGA ACTCCAGCGC CAGAAAGAAA TAATGAGACA GAGGCAGCAG 
CAGCAGGAGG CTTTACGACG GCTGCAACAA CAGCAGCAAC AGCAGCAACT TGCACAAATG 
AAGCTCCCTT CATCTTCGAC ATGGGGTCAG CAATCAAATT CAGGAGCTTG TCAATCCCAG 
ACCACACTGT CATTGGCAGA AATCCAAAAG TTAGAAGAAG AACGAGAACG GCAGCTTCGA 
GAAGAGCAAA GGCGTCAGCA GCGTGAACTA ATGAAGGCCC TCCAGCAACA GCAGCAGCAA 
CAGCAGCAAA AATTGTCAGG CTGGGGTAAT GTCACCAAAC CAACAGGCAC CACCAAGTCC 
CTGCTGGAGA TACAGCAGGA GGAGGCAAGG CAGATGCAGA AACAGCAGCA ACAGCAGCAC 
CAGCAGCCGA ACAGAGTCAG AAATACTACG CACTCTAACC TGCACACCAG CATTGGGAAT 
TCAGTGTGGG GCTCCCTAAA CACTAATCCC AGCAACCAGT GGGCATCTGA CCTAGTCAGT 
AGCATCTGGA GTAATGCTGA TACCAAAAAC TCAAACATGG GTTTCTGGGA TGATGCAGTG 
AAGGAAGTGG GACCTCGTAA CTCGACCAAT AAAAACAAAA GCAATGCCAG CCTCAGTAAA 
TCTGTGGGTC TTACAAGTAG ACAAAACAAG AAGGTGGAAG AAGAGGAGAA GCTGTTGAAA 
TTGTTCCAGG GGGTTAATAA AGCCCAGGAT GGATTCACTC AGTGGTGTGA ACAGATGCTT 
CATGCACTCA ACACAGCCAA CAACTTAGAT GTTCCCACGT TTGTTTCTTT CCTGAAGGAA 
GTGGAGTCTC CCTACGAGGT CCATGATTAC ATCAGAGCCT ATCTTGGAGA TACTCCTGAG 
GCCAAGGAGT TTGCCAAGCA GTTTCTCGAG CGCCGTGCCA AACAGAAAGC CAGTCAGCAG 
CGACAACAGC AGCAGCAACA GGATTCAGTG TGGGGGATGA ACCACAGTTC GCTACATTCG 
GTCTTTCAGA CCAACCAGAG CAACAACCAG CAATCCAACT TTGAGGCTGT GCAGAGCGGG 
AAGAAGAAGA AGAAACAGAA AATGGTTCGC GCAGATCCCA GCTTGCTAGG GTTTTCAGTG 
AATGCCTCGT CAGAGCGGCT CAATATGGGA GAAATAGAGA CTTTGGAAGA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952139.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807625.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X9, mRNA.

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CloneID	OCa72430
Clone ID Related Accession (Same CDS sequence)	XM_014952139.1
Accession Version	XM_014952139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGG CTGGCGAGAG 
CACATGGAGC GGCGGCGGAG GTTTGAGTTC GATTTCCGGG AACGAGACGA CGAGCGGGGC 
TATCGACGGG TACGGTGTGG CAGCGGCAGC ATGGACGACG ACAGAGACAG TCTCCCCGAG 
TGGTGCTTAG AAGATGCAGA AGAAGAAATG GGAACATTTG ACTCCTCCGG AGCGTTTCTC 
TCTTTAAAGA AGGTGCAGAA GGAGCCAATC CCTGAGGAAC AGGAGATGGA CTTCAGACCT 
GTGGAGGAAG GGGAAGAAAG ATCAGACTCT GAAGGGAGCC ACAACGAAGA AGCCAAAGAT 
CATGAAAAGA CCACCAAGAA AGAGGGGGAG AAAACAGAGA GAGTGGTGGC AGAAACAGTG 
GAAGAAGCAG CCCAAACATC TTCACCGGCT ACCAGGTCAG ATACCCCACC GAAATCCCAG 
CCTCCAGACC CGCTGCAGAC GAGCCTCTTT GAAAGGAAGG AAGAGTCTGT GCCAGAAAGA 
ACAGAAAAAA CAGAAGACAA AGAGAATCGA ACAGAGAATA CACCACCAGC CAAAATGTCC 
AACAGAGGGG AAGATTTGGC TTCTGTTGCT CAACCTTTGC CACAGATTTC TGCAGACACA 
GCTTCTCCTG CTCATCTTTC TCCTCCTGTT TCCAATTCAA ATCCTGCTCT GCGGCCAGTT 
CAAACACCTG TCACAGCTGC TCCAGGCATG GGCAACATTC CCACTGACCC AGATGATGAA 
GAGGGCCTCA AACACCTAGA GCAGCAAGCA GAGAAGATGG TGGCATACCT GCAGGACAGT 
GCCCTTGATG ATGAAAGGCT AGCAGCAAAA ATTCAAGAGC ACAGAGCAAA GGGTTCCTCT 
ATGCCGCTGT TCCATGAAGC CATGCAGAAA TGGTACTACA AAGATCCACA AGGAGAAATT 
CAGGGTCCTT TCAGTAATCA GGAGATGGCA GAGTGGTTCC AGGCCGGATA CTTCACCATG 
TCGCTGTTGG TCAAGAGAGC CTGCGATGAG ACTTTCCAGC CACTGGGAGA CATCATGAAA 
ATGTGGGGCA GGGTACCCTT CACTCCAGGT CCAGCACCGC TTCCACACCT GGGCGAGCTG 
GACCAAGAGC GGCTGACTAG GCAACAAGAG TTGGCCCTGA TCCAAATGCA GCACCTCCAG 
TATCAGCATC TTCTAATACA ACAACAATAT GCCCAGGTGC TGGCCCAGCA GCAAAAGGCA 
GCCCTCTCAT CCCAGCAGCA GCAGCAGCTC GCTATTCTCT TGCAACAGTT CCAGGCCCTG 
AAGATGAGAA TATCTGAACA GAACATGATG CCACCTGTTA CAAGGTCTGT GTCAGTGCCG 
GACACTGGCT CAATATGGGA ACTTCAGTCA GCTTCACAGC CTACAGTCTG GGAAGGAAGC 
AGTGTCTGGG ATCTCCCCAT TGATACTGCA GCTCAGGGAC CAGCTCTAGA ACAACTTCAG 
CAGCTCGAGA AGGCCAAAGC TGCAAAGCTA GAGCAAGAGA GGAGAGAAGT GGAAATGAGG 
GCCAAACGAG AGGAAGAGGA GAGGAAAAGG CAGGAGGAGC TTCGGAGGCA ACAAGAAGAG 
CTACTTAGGA GGCAGCAGGA AGATGAGAGG AAACGACGGG AAGAAGAAGA ACTGGCCCGC 
AGGAAGCAAG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807625.1
CDS	122..3940
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952139.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952139.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGG CTGGCGAGAG 
CACATGGAGC GGCGGCGGAG GTTTGAGTTC GATTTCCGGG AACGAGACGA CGAGCGGGGC 
TATCGACGGG TACGGTGTGG CAGCGGCAGC ATGGACGACG ACAGAGACAG TCTCCCCGAG 
TGGTGCTTAG AAGATGCAGA AGAAGAAATG GGAACATTTG ACTCCTCCGG AGCGTTTCTC 
TCTTTAAAGA AGGTGCAGAA GGAGCCAATC CCTGAGGAAC AGGAGATGGA CTTCAGACCT 
GTGGAGGAAG GGGAAGAAAG ATCAGACTCT GAAGGGAGCC ACAACGAAGA AGCCAAAGAT 
CATGAAAAGA CCACCAAGAA AGAGGGGGAG AAAACAGAGA GAGTGGTGGC AGAAACAGTG 
GAAGAAGCAG CCCAAACATC TTCACCGGCT ACCAGGTCAG ATACCCCACC GAAATCCCAG 
CCTCCAGACC CGCTGCAGAC GAGCCTCTTT GAAAGGAAGG AAGAGTCTGT GCCAGAAAGA 
ACAGAAAAAA CAGAAGACAA AGAGAATCGA ACAGAGAATA CACCACCAGC CAAAATGTCC 
AACAGAGGGG AAGATTTGGC TTCTGTTGCT CAACCTTTGC CACAGATTTC TGCAGACACA 
GCTTCTCCTG CTCATCTTTC TCCTCCTGTT TCCAATTCAA ATCCTGCTCT GCGGCCAGTT 
CAAACACCTG TCACAGCTGC TCCAGGCATG GGCAACATTC CCACTGACCC AGATGATGAA 
GAGGGCCTCA AACACCTAGA GCAGCAAGCA GAGAAGATGG TGGCATACCT GCAGGACAGT 
GCCCTTGATG ATGAAAGGCT AGCAGCAAAA ATTCAAGAGC ACAGAGCAAA GGGTTCCTCT 
ATGCCGCTGT TCCATGAAGC CATGCAGAAA TGGTACTACA AAGATCCACA AGGAGAAATT 
CAGGGTCCTT TCAGTAATCA GGAGATGGCA GAGTGGTTCC AGGCCGGATA CTTCACCATG 
TCGCTGTTGG TCAAGAGAGC CTGCGATGAG ACTTTCCAGC CACTGGGAGA CATCATGAAA 
ATGTGGGGCA GGGTACCCTT CACTCCAGGT CCAGCACCGC TTCCACACCT GGGCGAGCTG 
GACCAAGAGC GGCTGACTAG GCAACAAGAG TTGGCCCTGA TCCAAATGCA GCACCTCCAG 
TATCAGCATC TTCTAATACA ACAACAATAT GCCCAGGTGC TGGCCCAGCA GCAAAAGGCA 
GCCCTCTCAT CCCAGCAGCA GCAGCAGCTC GCTATTCTCT TGCAACAGTT CCAGGCCCTG 
AAGATGAGAA TATCTGAACA GAACATGATG CCACCTGTTA CAAGGTCTGT GTCAGTGCCG 
GACACTGGCT CAATATGGGA ACTTCAGTCA GCTTCACAGC CTACAGTCTG GGAAGGAAGC 
AGTGTCTGGG ATCTCCCCAT TGATACTGCA GCTCAGGGAC CAGCTCTAGA ACAACTTCAG 
CAGCTCGAGA AGGCCAAAGC TGCAAAGCTA GAGCAAGAGA GGAGAGAAGT GGAAATGAGG 
GCCAAACGAG AGGAAGAGGA GAGGAAAAGG CAGGAGGAGC TTCGGAGGCA ACAAGAAGAG 
CTACTTAGGA GGCAGCAGGA AGATGAGAGG AAACGACGGG AAGAAGAAGA ACTGGCCCGC 
AGGAAGCAAG AGGAAGCCCT GAGGCGACAA CGAGAGCAGG AAATTGCACT AAGGCGGCAG 
CGGGAGGAAG AGGAGCGGCA ACAGCAGGAG GAAGCACTTA GAAGACTGGA AGAGAGAAGA 
AGAGAAGAGG AGGAAAGACG GCAGCGAGAG GAGTTAATTC GTAAACAGGA AGAGGAGGCT 
GCCAAGTGGG CTCGTGAAGA GGAGGAAGCT CAACGGCGAC TTGAAGAAAG TCGGCTACGC 
ATGGAAGAAG AGGCAGCCAG ACAGCGGCTC GAAGAAGAAG AGCGAAAGCG CAAGGAGCTG 
GAACTCCAGC GCCAGAAAGA AATAATGAGA CAGAGGCAGC AGCAGCAGGA GGCTTTACGA 
CGGCTGCAAC AACAGCAGCA ACAGCAGCAA CTTGCACAAA TGAAGCTCCC TTCATCTTCG 
ACATGGGGTC AGCAATCAAA TTCAGGAGCT TGTCAATCCC AGACCACACT GTCATTGGCA 
GAAATCCAAA AGTTAGAAGA AGAACGAGAA CGGCAGCTTC GAGAAGAGCA AAGGCGTCAG 
CAGCGTGAAC TAATGAAGGC CCTCCAGCAA CAGCAGCAGC AACAGCAGCA AAAATTGTCA 
GGCTGGGGTA ATGTCACCAA ACCAACAGGC ACCACCAAGT CCCTGCTGGA GATACAGCAG 
GAGGAGGCAA GGCAGATGCA GAAACAGCAG CAACAGCAGC ACCAGCAGCC GAACAGAGTC 
AGAAATACTA CGCACTCTAA CCTGCACACC AGCATTGGGA ATTCAGTGTG GGGCTCCCTA 
AACACTAATC CCAGCAACCA GTGGGCATCT GACCTAGTCA GTAGCATCTG GAGTAATGCT 
GATACCAAAA ACTCAAACAT GGGTTTCTGG GATGATGCAG TGAAGGAAGT GGGACCTCGT 
AACTCGACCA ATAAAAACAA AAGCAATGCC AGCCTCAGTA AATCTGTGGG TCTTACAAGT 
AGACAAAACA AGAAGGTGGA AGAAGAGGAG AAGCTGTTGA AATTGTTCCA GGGGGTTAAT 
AAAGCCCAGG ATGGATTCAC TCAGTGGTGT GAACAGATGC TTCATGCACT CAACACAGCC 
AACAACTTAG ATGTTCCCAC GTTTGTTTCT TTCCTGAAGG AAGTGGAGTC TCCCTACGAG 
GTCCATGATT ACATCAGAGC CTATCTTGGA GATACTCCTG AGGCCAAGGA GTTTGCCAAG 
CAGTTTCTCG AGCGCCGTGC CAAACAGAAA GCCAGTCAGC AGCGACAACA GCAGCAGCAA 
CAGGATTCAG TGTGGGGGAT GAACCACAGT TCGCTACATT CGGTCTTTCA GACCAACCAG 
AGCAACAACC AGCAATCCAA CTTTGAGGCT GTGCAGAGCG GGAAGAAGAA GAAGAAACAG 
AAAATGGTTC GCGCAGATCC CAGCTTGCTA GGGTTTTCAG TGAATGCCTC GTCAGAGCGG 
CTCAATATGG GAGAAATAGA GACTTTGGAA GACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952140.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807626.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCa72431
Clone ID Related Accession (Same CDS sequence)	XM_014952140.1
Accession Version	XM_014952140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGTGAG ACCAAACTTT GAGGAAGGTG GGACAGCAAC GACGGGGAGG 
AAGCATGAGT TTATACGCTC TGAGAGCGAG AACTGGCGCA TCTTCAGGGA GGAACAAAAT 
GGGGAAGATG AAGATGGAGG CTGGCGACTG GCTGGGTCAC GGAGGGATGG TGAGAGGTGG 
CGTCCTCACA GTCCAGACGG CCCTCGTTCC GCAGGCTGGC GAGAGCACAT GGAGCGGCGG 
CGGAGGTTTG AGTTCGATTT CCGGGAACGA GACGACGAGC GGGGCTATCG ACGGGTACGG 
TGTGGCAGCG GCAGCATGGA CGACGACAGA GACAGTCTCC CCGAGTGGTG CTTAGAAGAT 
GCAGAAGAAG AAATGGGAAC ATTTGACTCC TCCGGAGCGT TTCTCTCTTT AAAGAAGGTG 
CAGAAGGAGC CAATCCCTGA GGAACAGGAG ATGGACTTCA GACCTGTGGA GGAAGGGGAA 
GAAAGATCAG ACTCTGAAGG GAGCCACAAC GAAGAAGCCA AAGATCATGA AAAGACCACC 
AAGAAAGAGG GGGAGAAAAC AGAGAGAGTG GTGGCAGAAA CAGTGGAAGA AGCAGCCCAA 
ACATCTTCAC CGGCTACCAG GTCAGATACC CCACCGAAAT CCCAGCCTCC AGACCCGCTG 
CAGACGAGCC TCTTTGAAAG GAAGGAAGAG TCTGTGCCAG AAAGAACAGA AAAAACAGAA 
GACAAAGAGA ATCGAACAGA GAATACACCA CCAGCCAAAA TGTCCAACAG AGGGGAAGAT 
TTGGCTTCTG TTGCTCAACC TTTGCCACAG ATTTCTGCAG ACACAGCTTC TCCTGCTCAT 
CTTTCTCCTC CTGTTTCCAA TTCAAATCCT GCTCTGCGGC CAGTTCAAAC ACCTGTCACA 
GCTGCTCCAG GCATGGGCAA CATTCCCACT GACCCAGATG ATGAAGAGGG CCTCAAACAC 
CTAGAGCAGC AAGCAGAGAA GATGGTGGCA TACCTGCAGG ACAGTGCCCT TGATGATGAA 
AGGCTAGCAG CAAAAATTCA AGAGCACAGA GCAAAGGGTT CCTCTATGCC GCTGTTCCAT 
GAAGCCATGC AGAAATGGTA CTACAAAGAT CCACAAGGAG AAATTCAGGG TCCTTTCAGT 
AATCAGGAGA TGGCAGAGTG GTTCCAGGCC GGATACTTCA CCATGTCGCT GTTGGTCAAG 
AGAGCCTGCG ATGAGACTTT CCAGCCACTG GGAGACATCA TGAAAATGTG GGGCAGGGTA 
CCCTTCACTC CAGGTCCAGC ACCGCTTCCA CACCTGGGCG AGCTGGACCA AGAGCGGCTG 
ACTAGGCAAC AAGAGTTGGC CCTGATCCAA ATGCAGCACC TCCAGTATCA GCATCTTCTA 
ATACAACAAC AATATGCCCA GGTGCTGGCC CAGCAGCAAA AGGCAGCCCT CTCATCCCAG 
CAGCAGCAGC AGCTCGCTAT TCTCTTGCAA CAGTTCCAGG CCCTGAAGAT GAGAATATCT 
GAACAGAACA TGATGCCACC TGTTACAAGG TCTGTGTCAG TGCCGGACAC TGGCTCAATA 
TGGGAACTTC AGTCAGCTTC ACAGCCTACA GTCTGGGAAG GAAGCAGTGT CTGGGATCTC 
CCCATTGATA CTGCAGCTCA GGGACCAGCT CTAGAACAAC TTCAGCAGCT CGAGAAGGCC 
AAAGCTGCAA AGCTAGAGCA AGAGAGGAGA GAAGTGGAAA TGAGGGCCAA ACGAGAGGAA 
GAGGAGAGGA AAAGGCAGGA GGAGCTTCGG AGGCAACAAG AAGAGCTACT TAGGAGGCAG 
CAGGAAGATG AGAGGAAACG ACGGGAAGAA GAAGAACTGG CCCGCAGGAA GCAAGAGATG 
ACAGTGAGAG CTGAGAGGGG TGAAGCAGTA GACTTAATCT GCCTGGACTT CAGGAAGGCT 
TTTGATGTAA TACTGCATAA AAGACTAGTA TATAAGGAGG AAGCCCTGAG GCGACAACGA 
GAGCAGGAAA TTGCACTAAG GCGGCAGCGG GAGGAAGAGG AGCGGCAACA GCAGGAGGAA 
GCACTTAGAA GACTGGAAGA GAGAAGAAGA GAAGAGGAGG AAAGACGGCA GCGAGAGGAG 
TTAATTCGTA AACAGGAAGA GGAGGCTGCC AAGTGGGCTC GTGAAGAGGA GGAAGCTCAA 
CGGCGACTTG AAGAAAGTCG GCTACGCATG GAAGAAGAGG CAGCCAGACA GCGGCTCGAA 
GAAGAAGAGC GAAAGCGCAA GGAGCTGGAA CTCCAGCGCC AGAAAGAAAT AATGAGACAG 
AGGCAGCAGC AGCAGGAGGC TTTACGACGG CTGCAACAAC AGCAGCAACA GCAGCAACTT 
GCACAAATGA AGCTCCCTTC ATCTTCGACA TGGGGTCAGC AATCAAATTC AGGAGCTTGT 
CAATCCCAGA CCACACTGTC ATTGGCAGAA ATCCAAAAGT TAGAAGAAGA ACGAGAACGG 
CAGCTTCGAG AAGAGCAAAG GCGTCAGCAG CGTGAACTAA TGAAGGCCCT CCAGCAACAG 
CAGCAGCAAC AGCAGCAAAA ATTGTCAGGC TGGGGTAATG TCACCAAACC AACAGGCACC 
ACCAAGTCCC TGCTGGAGAT ACAGCAGGAG GAGGCAAGGC AGATGCAGAA ACAGCAGCAA 
CAGCAGCACC AGCAGCCGAA CAGAGTCAGA AATACTACGC ACTCTAACCT GCACACCAGC 
ATTGGGAATT CAGTGTGGGG CTCCCTAAAC ACTAATCCCA GCAACCAGTG GGCATCTGAC 
CTAGTCAGTA GCATCTGGAG TAATGCTGAT ACCAAAAACT CAAACATGGG TTTCTGGGAT 
GATGCAGTGA AGGAAGTGGG ACCTCGTAAC TCGACCAATA AAAACAAAAG CAATGCCAGC 
CTCAGTAAAT CTGTGGGTCT TACAAGTAGA CAAAACAAGA AGGTGGAAGA AGAGGAGAAG 
CTGTTGAAAT TGTTCCAGGG GGTTAATAAA GCCCAGGATG GATTCACTCA GTGGTGTGAA 
CAGATGCTTC ATGCACTCAA CACAGCCAAC AACTTAGATG TTCCCACGTT TGTTTCTTTC 
CTGAAGGAAG TGGAGTCTCC CTACGAGGTC CATGATTACA TCAGAGCCTA TCTTGGAGAT 
ACTCCTGAGG CCAAGGAGTT TGCCAAGCAG TTTCTCGAGC GCCGTGCCAA ACAGAAAGCC 
AGTCAGCAGC GACAACAGCA GCAGCAACAG GATTCAGTGT GGGGGATGAA CCACAGTTCG 
CTACATTCGG TCTTTCAGAC CAACCAGAGC AACAACCAGC AATCCAACTT TGAGGCTGTG 
CAGAGCGGGA AGAAGAAGAA GAAACAGAAA ATGGTTCGCG CAGATCCCAG CTTGCTAGGG 
TTTTCAGTGA ATGCCTCGTC AGAGCGGCTC AATATGGGAG AAATAGAGAC TTTGGAAGAC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807626.1
CDS	124..3909
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKLEQERREVEMRAKREEEERKRQEELRRQQEELLRRQQEDERKRREEEELARRKQEMTVRAERGEAVDLICLDFRKAFDVILHKRLVYKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952140.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952140.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGTGAG ACCAAACTTT GAGGAAGGTG GGACAGCAAC GACGGGGAGG 
AAGCATGAGT TTATACGCTC TGAGAGCGAG AACTGGCGCA TCTTCAGGGA GGAACAAAAT 
GGGGAAGATG AAGATGGAGG CTGGCGACTG GCTGGGTCAC GGAGGGATGG TGAGAGGTGG 
CGTCCTCACA GTCCAGACGG CCCTCGTTCC GCAGGCTGGC GAGAGCACAT GGAGCGGCGG 
CGGAGGTTTG AGTTCGATTT CCGGGAACGA GACGACGAGC GGGGCTATCG ACGGGTACGG 
TGTGGCAGCG GCAGCATGGA CGACGACAGA GACAGTCTCC CCGAGTGGTG CTTAGAAGAT 
GCAGAAGAAG AAATGGGAAC ATTTGACTCC TCCGGAGCGT TTCTCTCTTT AAAGAAGGTG 
CAGAAGGAGC CAATCCCTGA GGAACAGGAG ATGGACTTCA GACCTGTGGA GGAAGGGGAA 
GAAAGATCAG ACTCTGAAGG GAGCCACAAC GAAGAAGCCA AAGATCATGA AAAGACCACC 
AAGAAAGAGG GGGAGAAAAC AGAGAGAGTG GTGGCAGAAA CAGTGGAAGA AGCAGCCCAA 
ACATCTTCAC CGGCTACCAG GTCAGATACC CCACCGAAAT CCCAGCCTCC AGACCCGCTG 
CAGACGAGCC TCTTTGAAAG GAAGGAAGAG TCTGTGCCAG AAAGAACAGA AAAAACAGAA 
GACAAAGAGA ATCGAACAGA GAATACACCA CCAGCCAAAA TGTCCAACAG AGGGGAAGAT 
TTGGCTTCTG TTGCTCAACC TTTGCCACAG ATTTCTGCAG ACACAGCTTC TCCTGCTCAT 
CTTTCTCCTC CTGTTTCCAA TTCAAATCCT GCTCTGCGGC CAGTTCAAAC ACCTGTCACA 
GCTGCTCCAG GCATGGGCAA CATTCCCACT GACCCAGATG ATGAAGAGGG CCTCAAACAC 
CTAGAGCAGC AAGCAGAGAA GATGGTGGCA TACCTGCAGG ACAGTGCCCT TGATGATGAA 
AGGCTAGCAG CAAAAATTCA AGAGCACAGA GCAAAGGGTT CCTCTATGCC GCTGTTCCAT 
GAAGCCATGC AGAAATGGTA CTACAAAGAT CCACAAGGAG AAATTCAGGG TCCTTTCAGT 
AATCAGGAGA TGGCAGAGTG GTTCCAGGCC GGATACTTCA CCATGTCGCT GTTGGTCAAG 
AGAGCCTGCG ATGAGACTTT CCAGCCACTG GGAGACATCA TGAAAATGTG GGGCAGGGTA 
CCCTTCACTC CAGGTCCAGC ACCGCTTCCA CACCTGGGCG AGCTGGACCA AGAGCGGCTG 
ACTAGGCAAC AAGAGTTGGC CCTGATCCAA ATGCAGCACC TCCAGTATCA GCATCTTCTA 
ATACAACAAC AATATGCCCA GGTGCTGGCC CAGCAGCAAA AGGCAGCCCT CTCATCCCAG 
CAGCAGCAGC AGCTCGCTAT TCTCTTGCAA CAGTTCCAGG CCCTGAAGAT GAGAATATCT 
GAACAGAACA TGATGCCACC TGTTACAAGG TCTGTGTCAG TGCCGGACAC TGGCTCAATA 
TGGGAACTTC AGTCAGCTTC ACAGCCTACA GTCTGGGAAG GAAGCAGTGT CTGGGATCTC 
CCCATTGATA CTGCAGCTCA GGGACCAGCT CTAGAACAAC TTCAGCAGCT CGAGAAGGCC 
AAAGCTGCAA AGCTAGAGCA AGAGAGGAGA GAAGTGGAAA TGAGGGCCAA ACGAGAGGAA 
GAGGAGAGGA AAAGGCAGGA GGAGCTTCGG AGGCAACAAG AAGAGCTACT TAGGAGGCAG 
CAGGAAGATG AGAGGAAACG ACGGGAAGAA GAAGAACTGG CCCGCAGGAA GCAAGAGATG 
ACAGTGAGAG CTGAGAGGGG TGAAGCAGTA GACTTAATCT GCCTGGACTT CAGGAAGGCT 
TTTGATGTAA TACTGCATAA AAGACTAGTA TATAAGGAGG AAGCCCTGAG GCGACAACGA 
GAGCAGGAAA TTGCACTAAG GCGGCAGCGG GAGGAAGAGG AGCGGCAACA GCAGGAGGAA 
GCACTTAGAA GACTGGAAGA GAGAAGAAGA GAAGAGGAGG AAAGACGGCA GCGAGAGGAG 
TTAATTCGTA AACAGGAAGA GGAGGCTGCC AAGTGGGCTC GTGAAGAGGA GGAAGCTCAA 
CGGCGACTTG AAGAAAGTCG GCTACGCATG GAAGAAGAGG CAGCCAGACA GCGGCTCGAA 
GAAGAAGAGC GAAAGCGCAA GGAGCTGGAA CTCCAGCGCC AGAAAGAAAT AATGAGACAG 
AGGCAGCAGC AGCAGGAGGC TTTACGACGG CTGCAACAAC AGCAGCAACA GCAGCAACTT 
GCACAAATGA AGCTCCCTTC ATCTTCGACA TGGGGTCAGC AATCAAATTC AGGAGCTTGT 
CAATCCCAGA CCACACTGTC ATTGGCAGAA ATCCAAAAGT TAGAAGAAGA ACGAGAACGG 
CAGCTTCGAG AAGAGCAAAG GCGTCAGCAG CGTGAACTAA TGAAGGCCCT CCAGCAACAG 
CAGCAGCAAC AGCAGCAAAA ATTGTCAGGC TGGGGTAATG TCACCAAACC AACAGGCACC 
ACCAAGTCCC TGCTGGAGAT ACAGCAGGAG GAGGCAAGGC AGATGCAGAA ACAGCAGCAA 
CAGCAGCACC AGCAGCCGAA CAGAGTCAGA AATACTACGC ACTCTAACCT GCACACCAGC 
ATTGGGAATT CAGTGTGGGG CTCCCTAAAC ACTAATCCCA GCAACCAGTG GGCATCTGAC 
CTAGTCAGTA GCATCTGGAG TAATGCTGAT ACCAAAAACT CAAACATGGG TTTCTGGGAT 
GATGCAGTGA AGGAAGTGGG ACCTCGTAAC TCGACCAATA AAAACAAAAG CAATGCCAGC 
CTCAGTAAAT CTGTGGGTCT TACAAGTAGA CAAAACAAGA AGGTGGAAGA AGAGGAGAAG 
CTGTTGAAAT TGTTCCAGGG GGTTAATAAA GCCCAGGATG GATTCACTCA GTGGTGTGAA 
CAGATGCTTC ATGCACTCAA CACAGCCAAC AACTTAGATG TTCCCACGTT TGTTTCTTTC 
CTGAAGGAAG TGGAGTCTCC CTACGAGGTC CATGATTACA TCAGAGCCTA TCTTGGAGAT 
ACTCCTGAGG CCAAGGAGTT TGCCAAGCAG TTTCTCGAGC GCCGTGCCAA ACAGAAAGCC 
AGTCAGCAGC GACAACAGCA GCAGCAACAG GATTCAGTGT GGGGGATGAA CCACAGTTCG 
CTACATTCGG TCTTTCAGAC CAACCAGAGC AACAACCAGC AATCCAACTT TGAGGCTGTG 
CAGAGCGGGA AGAAGAAGAA GAAACAGAAA ATGGTTCGCG CAGATCCCAG CTTGCTAGGG 
TTTTCAGTGA ATGCCTCGTC AGAGCGGCTC AATATGGGAG AAATAGAGAC TTTGGAAGAC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_014952141.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807627.1 Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCa72432
Clone ID Related Accession (Same CDS sequence)	XM_014952141.1
Accession Version	XM_014952141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090956.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGGAGGA AGCCCTGAGG 
CGACAACGAG AGCAGGAAAT TGCACTAAGG CGGCAGCGGG AGGAAGAGGA GCGGCAACAG 
CAGGAGGAAG CACTTAGAAG ACTGGAAGAG AGAAGAAGAG AAGAGGAGGA AAGACGGCAG 
CGAGAGGAGT TAATTCGTAA ACAGGAAGAG GAGGCTGCCA AGTGGGCTCG TGAAGAGGAG 
GAAGCTCAAC GGCGACTTGA AGAAAGTCGG CTACGCATGG AAGAAGAGGC AGCCAGACAG 
CGGCTCGAAG AAGAAGAGCG AAAGCGCAAG GAGCTGGAAC TCCAGCGCCA GAAAGAAATA 
ATGAGACAGA GGCAGCAGCA GCAGGAGGCT TTACGACGGC TGCAACAACA GCAGCAACAG 
CAGCAACTTG CACAAATGAA GCTCCCTTCA TCTTCGACAT GGGGTCAGCA ATCAAATTCA 
GGAGCTTGTC AATCCCAGAC CACACTGTCA TTGGCAGAAA TCCAAAAGTT AGAAGAAGAA 
CGAGAACGGC AGCTTCGAGA AGAGCAAAGG CGTCAGCAGC GTGAACTAAT GAAGGCCCTC 
CAGCAACAGC AGCAGCAACA GCAGCAAAAA TTGTCAGGCT GGGGTAATGT CACCAAACCA 
ACAGGCACCA CCAAGTCCCT GCTGGAGATA CAGCAGGAGG AGGCAAGGCA GATGCAGAAA 
CAGCAGCAAC AGCAGCACCA GCAGCCGAAC AGAGTCAGAA ATACTACGCA CTCTAACCTG 
CACACCAGCA TTGGGAATTC AGTGTGGGGC TCCCTAAACA CTAATCCCAG CAACCAGTGG 
GCATCTGACC TAGTCAGTAG CATCTGGAGT AATGCTGATA CCAAAAACTC AAACATGGGT 
TTCTGGGATG ATGCAGTGAA GGAAGTGGGA CCTCGTAACT CGACCAATAA AAACAAAAGC 
AATGCCAGCC TCAGTAAATC TGTGGGTCTT ACAAGTAGAC AAAACAAGAA GGTGGAAGAA 
GAGGAGAAGC TGTTGAAATT GTTCCAGGGG GTTAATAAAG CCCAGGATGG ATTCACTCAG 
TGGTGTGAAC AGATGCTTCA TGCACTCAAC ACAGCCAACA ACTTAGATGT TCCCACGTTT 
GTTTCTTTCC TGAAGGAAGT GGAGTCTCCC TACGAGGTCC ATGATTACAT CAGAGCCTAT 
CTTGGAGATA CTCCTGAGGC CAAGGAGTTT GCCAAGCAGT TTCTCGAGCG CCGTGCCAAA 
CAGAAAGCCA GTCAGCAGCG ACAACAGCAG CAGCAACAGG ATTCAGTGTG GGGGATGAAC 
CACAGTTCGC TACATTCGGT CTTTCAGACC AACCAGAGCA ACAACCAGCA ATCCAACTTT 
GAGGCTGTGC AGAGCGGGAA GAAGAAGAAG AAACAGAAAA TGGTTCGCGC AGATCCCAGC 
TTGCTAGGGT TTTCAGTGAA TGCCTCGTCA GAGCGGCTCA ATATGGGAGA AATAGAGACT 
TTGGAAGACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807627.1
CDS	124..3798
Translation	MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVVGAPRGRSSSRGRGRGRGESGFYQRSFDEVEGGFGRGGGREMHRSQSWEERGDRRFEKPGRKDPVRPNFEEGGTATTGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRERDDERGYRRVRCGSGSMDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEERSDSEGSHNEEAKDHEKTTKKEGEKTERVVAETVEEAAQTSSPATRSDTPPKSQPPDPLQTSLFERKEESVPERTEKTEDKENRTENTPPAKMSNRGEDLASVAQPLPQISADTASPAHLSPPVSNSNPALRPVQTPVTAAPGMGNIPTDPDDEEGLKHLEQQAEKMVAYLQDSALDDERLAAKIQEHRAKGSSMPLFHEAMQKWYYKDPQGEIQGPFSNQEMAEWFQAGYFTMSLLVKRACDETFQPLGDIMKMWGRVPFTPGPAPLPHLGELDQERLTRQQELALIQMQHLQYQHLLIQQQYAQVLAQQQKAALSSQQQQQLAILLQQFQALKMRISEQNMMPPVTRSVSVPDTGSIWELQSASQPTVWEGSSVWDLPIDTAAQGPALEQLQQLEKAKAAKEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRQREELIRKQEEEAAKWAREEEEAQRRLEESRLRMEEEAARQRLEEEERKRKELELQRQKEIMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNSGACQSQTTLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVTKPTGTTKSLLEIQQEEARQMQKQQQQQHQQPNRVRNTTHSNLHTSIGNSVWGSLNTNPSNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGPRNSTNKNKSNASLSKSVGLTSRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTPEAKEFAKQFLERRAKQKASQQRQQQQQQDSVWGMNHSSLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETLEDY

Target ORF information:

RefSeq Version	XM_014952141.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952141.1

ORF Insert Sequence:

ATGGCAGCAG AAACTCAGAC ACTTAACTTT GGGCCTGAAT GGCTGCGAGC TTTGTCTAGT 
GGCGGGAGCG TGACATCCCC TCCTCTGTCT CCGGCTTTGC CAAAGTACAA ACTAGCAGAC 
TATCGCTATG GGAGAGAAGA AATGTTGGCA CTTTTTCTAA AGGATAATAA GATACCTTCA 
GACCTTCTGG ATAAGGAGTT TCTGCCTATT TTACAAGAGG AGCCCCTGCC ACCACTGGCA 
CTTGTACCTT TTACAGAAGA AGAACAGAGA AATTTCTCGA TGTCTGTAAA CAGTGCTGCT 
GTCCTGCGGT TGACGGGACG CGGAGGAGGA ACGGTGGTAG GGGCTCCTCG AGGTCGAAGT 
TCCTCTAGAG GTCGAGGCAG AGGCCGAGGA GAAAGCGGTT TCTACCAAAG AAGTTTTGAT 
GAAGTGGAGG GTGGATTTGG GCGAGGAGGG GGCAGGGAAA TGCACAGATC ACAGAGTTGG 
GAGGAAAGGG GAGACAGACG CTTTGAAAAA CCAGGGCGAA AAGATCCAGT GAGACCAAAC 
TTTGAGGAAG GTGGGACAGC AACGACGGGG AGGAAGCATG AGTTTATACG CTCTGAGAGC 
GAGAACTGGC GCATCTTCAG GGAGGAACAA AATGGGGAAG ATGAAGATGG AGGCTGGCGA 
CTGGCTGGGT CACGGAGGGA TGGTGAGAGG TGGCGTCCTC ACAGTCCAGA CGGCCCTCGT 
TCCGCAGGCT GGCGAGAGCA CATGGAGCGG CGGCGGAGGT TTGAGTTCGA TTTCCGGGAA 
CGAGACGACG AGCGGGGCTA TCGACGGGTA CGGTGTGGCA GCGGCAGCAT GGACGACGAC 
AGAGACAGTC TCCCCGAGTG GTGCTTAGAA GATGCAGAAG AAGAAATGGG AACATTTGAC 
TCCTCCGGAG CGTTTCTCTC TTTAAAGAAG GTGCAGAAGG AGCCAATCCC TGAGGAACAG 
GAGATGGACT TCAGACCTGT GGAGGAAGGG GAAGAAAGAT CAGACTCTGA AGGGAGCCAC 
AACGAAGAAG CCAAAGATCA TGAAAAGACC ACCAAGAAAG AGGGGGAGAA AACAGAGAGA 
GTGGTGGCAG AAACAGTGGA AGAAGCAGCC CAAACATCTT CACCGGCTAC CAGGTCAGAT 
ACCCCACCGA AATCCCAGCC TCCAGACCCG CTGCAGACGA GCCTCTTTGA AAGGAAGGAA 
GAGTCTGTGC CAGAAAGAAC AGAAAAAACA GAAGACAAAG AGAATCGAAC AGAGAATACA 
CCACCAGCCA AAATGTCCAA CAGAGGGGAA GATTTGGCTT CTGTTGCTCA ACCTTTGCCA 
CAGATTTCTG CAGACACAGC TTCTCCTGCT CATCTTTCTC CTCCTGTTTC CAATTCAAAT 
CCTGCTCTGC GGCCAGTTCA AACACCTGTC ACAGCTGCTC CAGGCATGGG CAACATTCCC 
ACTGACCCAG ATGATGAAGA GGGCCTCAAA CACCTAGAGC AGCAAGCAGA GAAGATGGTG 
GCATACCTGC AGGACAGTGC CCTTGATGAT GAAAGGCTAG CAGCAAAAAT TCAAGAGCAC 
AGAGCAAAGG GTTCCTCTAT GCCGCTGTTC CATGAAGCCA TGCAGAAATG GTACTACAAA 
GATCCACAAG GAGAAATTCA GGGTCCTTTC AGTAATCAGG AGATGGCAGA GTGGTTCCAG 
GCCGGATACT TCACCATGTC GCTGTTGGTC AAGAGAGCCT GCGATGAGAC TTTCCAGCCA 
CTGGGAGACA TCATGAAAAT GTGGGGCAGG GTACCCTTCA CTCCAGGTCC AGCACCGCTT 
CCACACCTGG GCGAGCTGGA CCAAGAGCGG CTGACTAGGC AACAAGAGTT GGCCCTGATC 
CAAATGCAGC ACCTCCAGTA TCAGCATCTT CTAATACAAC AACAATATGC CCAGGTGCTG 
GCCCAGCAGC AAAAGGCAGC CCTCTCATCC CAGCAGCAGC AGCAGCTCGC TATTCTCTTG 
CAACAGTTCC AGGCCCTGAA GATGAGAATA TCTGAACAGA ACATGATGCC ACCTGTTACA 
AGGTCTGTGT CAGTGCCGGA CACTGGCTCA ATATGGGAAC TTCAGTCAGC TTCACAGCCT 
ACAGTCTGGG AAGGAAGCAG TGTCTGGGAT CTCCCCATTG ATACTGCAGC TCAGGGACCA 
GCTCTAGAAC AACTTCAGCA GCTCGAGAAG GCCAAAGCTG CAAAGGAGGA AGCCCTGAGG 
CGACAACGAG AGCAGGAAAT TGCACTAAGG CGGCAGCGGG AGGAAGAGGA GCGGCAACAG 
CAGGAGGAAG CACTTAGAAG ACTGGAAGAG AGAAGAAGAG AAGAGGAGGA AAGACGGCAG 
CGAGAGGAGT TAATTCGTAA ACAGGAAGAG GAGGCTGCCA AGTGGGCTCG TGAAGAGGAG 
GAAGCTCAAC GGCGACTTGA AGAAAGTCGG CTACGCATGG AAGAAGAGGC AGCCAGACAG 
CGGCTCGAAG AAGAAGAGCG AAAGCGCAAG GAGCTGGAAC TCCAGCGCCA GAAAGAAATA 
ATGAGACAGA GGCAGCAGCA GCAGGAGGCT TTACGACGGC TGCAACAACA GCAGCAACAG 
CAGCAACTTG CACAAATGAA GCTCCCTTCA TCTTCGACAT GGGGTCAGCA ATCAAATTCA 
GGAGCTTGTC AATCCCAGAC CACACTGTCA TTGGCAGAAA TCCAAAAGTT AGAAGAAGAA 
CGAGAACGGC AGCTTCGAGA AGAGCAAAGG CGTCAGCAGC GTGAACTAAT GAAGGCCCTC 
CAGCAACAGC AGCAGCAACA GCAGCAAAAA TTGTCAGGCT GGGGTAATGT CACCAAACCA 
ACAGGCACCA CCAAGTCCCT GCTGGAGATA CAGCAGGAGG AGGCAAGGCA GATGCAGAAA 
CAGCAGCAAC AGCAGCACCA GCAGCCGAAC AGAGTCAGAA ATACTACGCA CTCTAACCTG 
CACACCAGCA TTGGGAATTC AGTGTGGGGC TCCCTAAACA CTAATCCCAG CAACCAGTGG 
GCATCTGACC TAGTCAGTAG CATCTGGAGT AATGCTGATA CCAAAAACTC AAACATGGGT 
TTCTGGGATG ATGCAGTGAA GGAAGTGGGA CCTCGTAACT CGACCAATAA AAACAAAAGC 
AATGCCAGCC TCAGTAAATC TGTGGGTCTT ACAAGTAGAC AAAACAAGAA GGTGGAAGAA 
GAGGAGAAGC TGTTGAAATT GTTCCAGGGG GTTAATAAAG CCCAGGATGG ATTCACTCAG 
TGGTGTGAAC AGATGCTTCA TGCACTCAAC ACAGCCAACA ACTTAGATGT TCCCACGTTT 
GTTTCTTTCC TGAAGGAAGT GGAGTCTCCC TACGAGGTCC ATGATTACAT CAGAGCCTAT 
CTTGGAGATA CTCCTGAGGC CAAGGAGTTT GCCAAGCAGT TTCTCGAGCG CCGTGCCAAA 
CAGAAAGCCA GTCAGCAGCG ACAACAGCAG CAGCAACAGG ATTCAGTGTG GGGGATGAAC 
CACAGTTCGC TACATTCGGT CTTTCAGACC AACCAGAGCA ACAACCAGCA ATCCAACTTT 
GAGGCTGTGC AGAGCGGGAA GAAGAAGAAG AAACAGAAAA TGGTTCGCGC AGATCCCAGC 
TTGCTAGGGT TTTCAGTGAA TGCCTCGTCA GAGCGGCTCA ATATGGGAGA AATAGAGACT 
TTGGAAGACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GIGYF2
Entrez Gene ID	106893868
Full Name	GRB10 interacting GYF protein 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GIGYF2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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