ORF » Species Summary » Calidris pugnax » SLC12A2 cDNA ORF clone

SLC12A2 cDNA ORF clone, Calidris pugnax(ruff)

The following SLC12A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa74402 XM_014954800.1
Latest version!		 Calidris pugnax solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCa74403 XM_014954801.1
Latest version!		 Calidris pugnax solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SLC12A2 ( XM_014954800.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014810286.1 Calidris pugnax solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCa74402
    Clone ID Related Accession (Same CDS sequence) XM_014954800.1
    Accession Version XM_014954800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product solute carrier family 12 member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGGGCA AGCAGCAGCA GCCGCAGCCG GAGGAGCTGC CTCCTCCCGG CCTCGCCGTC 
    CCGGCGGGCT CGGAGGAGGG CGGCAAGGAT GCGGCGGGCG GCGGTGCGCT GGGCAGACCG 
    CTGGGTCCCA CGCCCAGCCA GAGCCGCTTC CAGGTGGACC TGGTGGCCGA GGGCGCCGGC 
    CGGCCGCCCG AGGAATCCCG CAAGGCGAGC GGGGGCGGCG GCACGGCGGG GAAGGGCAGC 
    GAGGAAGCCA AGGGCAGGTT TCGGGTGAAC TTCGTGGACC CGTCGGCCGG TGACGAGAGC 
    CCCGGCGAGC CGGGGGGCGG CAGCTCGGAG GGCGGCAATG TCAGCTTCCA GAACGGCGGC 
    GACACGGTGC TGAGCGAGGG CAGCCTCCAC TCCGGCGGGC ACCACCACTA CTACTATGAC 
    ACCCACACCA ACACCTACTA CCTGCGCACC TTCGGCCACA ACACCATGGA CGCCGTGCCC 
    CGCATCGACC ACTACCGCCA CACGGCCGCC GACCTGGGCG AGAAGCTCAT CCGGCCCAGC 
    CTGGCCGAGC TGCACGATGA GCTGGACAAG GAGCCCTTTG AAGATGGCTA TGCAAATGGT 
    GAGGAAGGCA CACCAGCTGG AGAAGCAGCT GCCACTTACA CACCTGATAG CAAAGGAGTG 
    GTCAAGTTTG GCTGGATAAA AGGCGTATTG GTACGATGTA TGTTAAATAT TTGGGGTGTG 
    ATGCTCTTCA TCAGACTCTC ATGGATTGTA GGACAAGCTG GGATAGGTCT GTCTGTTGTG 
    GTTATTGGAA TGGCCACTGT TGTGACAACT ATTACAGGAC TGTCTACTTC AGCAATAGCC 
    ACAAATGGGT TTGTAAGAGG AGGAGGTGCA TATTACTTAA TTTCCAGAAG TCTGGGACCG 
    GAGTTTGGTG GTGCCATAGG TCTGATTTTT GCATTTGCCA ATGCTGTTGC AGTAGCAATG 
    TATGTTGTTG GCTTTGCAGA GACAGTTGTG GAGCTGCTCA AGGAAAATGG AACGTTGATG 
    GTGGATGAAA TGAATGACAT CAGAATCATA GGGGCTATCA CAGTGGTGGT ATTGCTGGGT 
    ATTTCTGTTG CCGGAATGGA ATGGGAGGCA AAAGCCCAGA TAGTCTTACT GGTGATCCTC 
    ATACTTGCTA TTGGAGACTT CATCATAGGC ACATTTATTC CTCTGGATAG CAAGAAGCCT 
    AAAGGTTTCT TTGGTTATAA AGCTGAAATA TTTATGGAGA ATTTTGGGCC AGATTTCCGA 
    GACGAGGAAA CTTTCTTTTC TGTATTTGCA ATTTTCTTCC CTGCTGCAAC TGGCATACTT 
    GCTGGTGCAA ATATCTCAGG TGATCTTGCG GATCCTCAGT CAGCCATACC TAAAGGAACG 
    TTGTTGGCCA TCTTAATTAC TACATTGGTT TACGCAGGAA TTGCAGTATC TGTAGGTTCC 
    TGTGTTGTTC GAGATGCCAC AGGAAACATT AATAACACCA TTGTCACTGA GCTGACAAAT 
    TGCACTACTG CAGCTTGCAA ATTAAACTAT GATTTCTCAT CCTGTGAGAC AGGGTGTCAC 
    TATGGGCTGA TGAACAATTT CCAGGTAATG AGCATGGTAT CGGGATTTGC ACCATTGATT 
    TCTGCAGGTA TTTTTTCAGC CACCCTGTCT TCTGCATTAG CATCTCTTGT GAGTGCACCT 
    AAGATCTTCC AGGCTTTGTG CAAGGACAAC ATTTATCCTG CATTTCAGAT GTTTGGTAAA 
    GGCTATGGGA AGAACAATGA ACCACTGAGA GGATATCTTC TAACGTTTTT AATTGCTCTT 
    GGATTCATAC TAATTGCTGA ACTGAACGTC ATTGCTCCAA TTATTTCTAA CTTCTTCTTG 
    GCCTCATATG CCTTGATTAA CTTCTCTGTA TTCCATGCTT CTCTTGCAAA ATCTCCAGGT 
    TGGCGCCCTG CATTCAAATA CTACAATATG TGGATTTCTC TTGTTGGAGC AATTCTGTGC 
    TGCATTGTAA TGTTTGTCAT TAATTGGTGG GCAGCACTTC TAACATATGT CATAGTCCTT 
    GGACTCTACA TTTATGTCAC CTACAAGAAA CCAGATGTGA ACTGGGGATC CTCAACACAA 
    GCTCTGACTT ACTTGAATGC ACTGCAGCAT TCTATTCGGC TCTCAGGAGT GGAAGATCAC 
    GTGAAAAACT TTAGACCTCA GTGCTTAATT ATGACAGGGC CTCCAAATGC ACGTCCAGCT 
    TTGCTTCACC TTGTCCATGC TTTCACCAAG AATGTGGGCT TGATGATCTG TGGCCATGTA 
    CATATGGGTC CTCGGAGGCA AGCTATGAAG GAACTTTCAA CTGATTTGGC TAAATATCAG 
    CGATGGCTGA TTAAAAACAA GATGAAGGCT TTCTATGCAC CAGTGCATGC AGAGGACTTG 
    AGGGATGGAG GACAATACTT AATGCAGGCT GCTGGTCTGG GTAGAATGAG ACCTAATACT 
    CTTGTTGTTG GATTTAAGAA AAACTGGAGA CAAAGTGACA TGAGAGATGT TGAAACCTAT 
    ATCAATTTAT TCCATGATGC CTTTGATGTT CAGTATGGCG TAGTTGTAAT CCGCCTAAAG 
    GAAGGCCTGG ACATTTCTCA CCTTCAGGGC CAAGAAGAGT TACTGTCATC CCAAGAAAAA 
    TCTCCGTGTA CCAAGGACGT CATAGTAAAA GTGGATTATA GCAAGAAGTC TGAGACAGAC 
    ACTTCTATAG TTTTTGCTCC ATCATCTAGT GAAAGAACTC CCACTCCACA TAAAGAGGAG 
    GACGAGGATG GAAAGACTCC AACACAACCA CTGTTGAAAA AAGATTCAAA GAGCCCATCT 
    TCTCCTTTAA ACTTGGCTGA CCAAAGACTT CTTGATGCTA GTACTCAGTT TCAGAAGAAA 
    CAAGGCAAAA GCAATATTGA TGTCTGGTGG CTCTTTGATG ATGGAGGTTT GACACTGTTG 
    ATTCCGTATC TTATTACAAC CAAGAAGAAG TGGAAAGACT GTAAGATCAG AGTGTTCATT 
    GGTGGAAAAA TAAACAGGAT TGATCATGAC AGAAGAGCGA TGGCTACTTT GCTGAGCAAA 
    TTTCGGATAG ACTTCTCGGA CATTATGGTT CTTGGGGATA TTAATACTAA GCCAAAGAAA 
    GAAAATATTG CAGCTTTTGA AGAAATGATA GAGCCCTTCC GACTTCATGA AGATGATAAA 
    GAACAAGAAA TTGCAGATAA AATGAAGGAG GATGAACCAT GGAGGATAAC AGATAACGAA 
    CTTGAGCTTT ACAAAACAAA GACTTACCGC CAGATCAGGC TAAATGAGTT GCTGAAAGAA 
    CATTCAAGTA CAGCTAACAT AATTGTCATG AGTTTGCCAG TTGCACGAAA AGGTGCCGTT 
    TCTAGCGCGC TGTACATGGC CTGGTTAGAA GCTCTTTCTA AAGATCTGCC ACCAATTCTC 
    CTTGTTCGTG GGAATCATCA GAGCGTTCTT ACCTTCTATT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014810286.1
    CDS54..3518
    Translation

    Target ORF information:

    RefSeq Version XM_014954800.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014954800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGGGCA AGCAGCAGCA GCCGCAGCCG GAGGAGCTGC CTCCTCCCGG CCTCGCCGTC 
    CCGGCGGGCT CGGAGGAGGG CGGCAAGGAT GCGGCGGGCG GCGGTGCGCT GGGCAGACCG 
    CTGGGTCCCA CGCCCAGCCA GAGCCGCTTC CAGGTGGACC TGGTGGCCGA GGGCGCCGGC 
    CGGCCGCCCG AGGAATCCCG CAAGGCGAGC GGGGGCGGCG GCACGGCGGG GAAGGGCAGC 
    GAGGAAGCCA AGGGCAGGTT TCGGGTGAAC TTCGTGGACC CGTCGGCCGG TGACGAGAGC 
    CCCGGCGAGC CGGGGGGCGG CAGCTCGGAG GGCGGCAATG TCAGCTTCCA GAACGGCGGC 
    GACACGGTGC TGAGCGAGGG CAGCCTCCAC TCCGGCGGGC ACCACCACTA CTACTATGAC 
    ACCCACACCA ACACCTACTA CCTGCGCACC TTCGGCCACA ACACCATGGA CGCCGTGCCC 
    CGCATCGACC ACTACCGCCA CACGGCCGCC GACCTGGGCG AGAAGCTCAT CCGGCCCAGC 
    CTGGCCGAGC TGCACGATGA GCTGGACAAG GAGCCCTTTG AAGATGGCTA TGCAAATGGT 
    GAGGAAGGCA CACCAGCTGG AGAAGCAGCT GCCACTTACA CACCTGATAG CAAAGGAGTG 
    GTCAAGTTTG GCTGGATAAA AGGCGTATTG GTACGATGTA TGTTAAATAT TTGGGGTGTG 
    ATGCTCTTCA TCAGACTCTC ATGGATTGTA GGACAAGCTG GGATAGGTCT GTCTGTTGTG 
    GTTATTGGAA TGGCCACTGT TGTGACAACT ATTACAGGAC TGTCTACTTC AGCAATAGCC 
    ACAAATGGGT TTGTAAGAGG AGGAGGTGCA TATTACTTAA TTTCCAGAAG TCTGGGACCG 
    GAGTTTGGTG GTGCCATAGG TCTGATTTTT GCATTTGCCA ATGCTGTTGC AGTAGCAATG 
    TATGTTGTTG GCTTTGCAGA GACAGTTGTG GAGCTGCTCA AGGAAAATGG AACGTTGATG 
    GTGGATGAAA TGAATGACAT CAGAATCATA GGGGCTATCA CAGTGGTGGT ATTGCTGGGT 
    ATTTCTGTTG CCGGAATGGA ATGGGAGGCA AAAGCCCAGA TAGTCTTACT GGTGATCCTC 
    ATACTTGCTA TTGGAGACTT CATCATAGGC ACATTTATTC CTCTGGATAG CAAGAAGCCT 
    AAAGGTTTCT TTGGTTATAA AGCTGAAATA TTTATGGAGA ATTTTGGGCC AGATTTCCGA 
    GACGAGGAAA CTTTCTTTTC TGTATTTGCA ATTTTCTTCC CTGCTGCAAC TGGCATACTT 
    GCTGGTGCAA ATATCTCAGG TGATCTTGCG GATCCTCAGT CAGCCATACC TAAAGGAACG 
    TTGTTGGCCA TCTTAATTAC TACATTGGTT TACGCAGGAA TTGCAGTATC TGTAGGTTCC 
    TGTGTTGTTC GAGATGCCAC AGGAAACATT AATAACACCA TTGTCACTGA GCTGACAAAT 
    TGCACTACTG CAGCTTGCAA ATTAAACTAT GATTTCTCAT CCTGTGAGAC AGGGTGTCAC 
    TATGGGCTGA TGAACAATTT CCAGGTAATG AGCATGGTAT CGGGATTTGC ACCATTGATT 
    TCTGCAGGTA TTTTTTCAGC CACCCTGTCT TCTGCATTAG CATCTCTTGT GAGTGCACCT 
    AAGATCTTCC AGGCTTTGTG CAAGGACAAC ATTTATCCTG CATTTCAGAT GTTTGGTAAA 
    GGCTATGGGA AGAACAATGA ACCACTGAGA GGATATCTTC TAACGTTTTT AATTGCTCTT 
    GGATTCATAC TAATTGCTGA ACTGAACGTC ATTGCTCCAA TTATTTCTAA CTTCTTCTTG 
    GCCTCATATG CCTTGATTAA CTTCTCTGTA TTCCATGCTT CTCTTGCAAA ATCTCCAGGT 
    TGGCGCCCTG CATTCAAATA CTACAATATG TGGATTTCTC TTGTTGGAGC AATTCTGTGC 
    TGCATTGTAA TGTTTGTCAT TAATTGGTGG GCAGCACTTC TAACATATGT CATAGTCCTT 
    GGACTCTACA TTTATGTCAC CTACAAGAAA CCAGATGTGA ACTGGGGATC CTCAACACAA 
    GCTCTGACTT ACTTGAATGC ACTGCAGCAT TCTATTCGGC TCTCAGGAGT GGAAGATCAC 
    GTGAAAAACT TTAGACCTCA GTGCTTAATT ATGACAGGGC CTCCAAATGC ACGTCCAGCT 
    TTGCTTCACC TTGTCCATGC TTTCACCAAG AATGTGGGCT TGATGATCTG TGGCCATGTA 
    CATATGGGTC CTCGGAGGCA AGCTATGAAG GAACTTTCAA CTGATTTGGC TAAATATCAG 
    CGATGGCTGA TTAAAAACAA GATGAAGGCT TTCTATGCAC CAGTGCATGC AGAGGACTTG 
    AGGGATGGAG GACAATACTT AATGCAGGCT GCTGGTCTGG GTAGAATGAG ACCTAATACT 
    CTTGTTGTTG GATTTAAGAA AAACTGGAGA CAAAGTGACA TGAGAGATGT TGAAACCTAT 
    ATCAATTTAT TCCATGATGC CTTTGATGTT CAGTATGGCG TAGTTGTAAT CCGCCTAAAG 
    GAAGGCCTGG ACATTTCTCA CCTTCAGGGC CAAGAAGAGT TACTGTCATC CCAAGAAAAA 
    TCTCCGTGTA CCAAGGACGT CATAGTAAAA GTGGATTATA GCAAGAAGTC TGAGACAGAC 
    ACTTCTATAG TTTTTGCTCC ATCATCTAGT GAAAGAACTC CCACTCCACA TAAAGAGGAG 
    GACGAGGATG GAAAGACTCC AACACAACCA CTGTTGAAAA AAGATTCAAA GAGCCCATCT 
    TCTCCTTTAA ACTTGGCTGA CCAAAGACTT CTTGATGCTA GTACTCAGTT TCAGAAGAAA 
    CAAGGCAAAA GCAATATTGA TGTCTGGTGG CTCTTTGATG ATGGAGGTTT GACACTGTTG 
    ATTCCGTATC TTATTACAAC CAAGAAGAAG TGGAAAGACT GTAAGATCAG AGTGTTCATT 
    GGTGGAAAAA TAAACAGGAT TGATCATGAC AGAAGAGCGA TGGCTACTTT GCTGAGCAAA 
    TTTCGGATAG ACTTCTCGGA CATTATGGTT CTTGGGGATA TTAATACTAA GCCAAAGAAA 
    GAAAATATTG CAGCTTTTGA AGAAATGATA GAGCCCTTCC GACTTCATGA AGATGATAAA 
    GAACAAGAAA TTGCAGATAA AATGAAGGAG GATGAACCAT GGAGGATAAC AGATAACGAA 
    CTTGAGCTTT ACAAAACAAA GACTTACCGC CAGATCAGGC TAAATGAGTT GCTGAAAGAA 
    CATTCAAGTA CAGCTAACAT AATTGTCATG AGTTTGCCAG TTGCACGAAA AGGTGCCGTT 
    TCTAGCGCGC TGTACATGGC CTGGTTAGAA GCTCTTTCTA AAGATCTGCC ACCAATTCTC 
    CTTGTTCGTG GGAATCATCA GAGCGTTCTT ACCTTCTATT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SLC12A2 ( XM_014954801.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014810287.1 Calidris pugnax solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCa74403
    Clone ID Related Accession (Same CDS sequence) XM_014954801.1
    Accession Version XM_014954801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product solute carrier family 12 member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAGGGCA AGCAGCAGCA GCCGCAGCCG GAGGAGCTGC CTCCTCCCGG CCTCGCCGTC 
    CCGGCGGGCT CGGAGGAGGG CGGCAAGGAT GCGGCGGGCG GCGGTGCGCT GGGCAGACCG 
    CTGGGTCCCA CGCCCAGCCA GAGCCGCTTC CAGGTGGACC TGGTGGCCGA GGGCGCCGGC 
    CGGCCGCCCG AGGAATCCCG CAAGGCGAGC GGGGGCGGCG GCACGGCGGG GAAGGGCAGC 
    GAGGAAGCCA AGGGCAGGTT TCGGGTGAAC TTCGTGGACC CGTCGGCCGG TGACGAGAGC 
    CCCGGCGAGC CGGGGGGCGG CAGCTCGGAG GGCGGCAATG TCAGCTTCCA GAACGGCGGC 
    GACACGGTGC TGAGCGAGGG CAGCCTCCAC TCCGGCGGGC ACCACCACTA CTACTATGAC 
    ACCCACACCA ACACCTACTA CCTGCGCACC TTCGGCCACA ACACCATGGA CGCCGTGCCC 
    CGCATCGACC ACTACCGCCA CACGGCCGCC GACCTGGGCG AGAAGCTCAT CCGGCCCAGC 
    CTGGCCGAGC TGCACGATGA GCTGGACAAG GAGCCCTTTG AAGATGGCTA TGCAAATGGT 
    GAGGAAGGCA CACCAGCTGG AGAAGCAGCT GCCACTTACA CACCTGATAG CAAAGGAGTG 
    GTCAAGTTTG GCTGGATAAA AGGCGTATTG GTACGATGTA TGTTAAATAT TTGGGGTGTG 
    ATGCTCTTCA TCAGACTCTC ATGGATTGTA GGACAAGCTG GGATAGGTCT GTCTGTTGTG 
    GTTATTGGAA TGGCCACTGT TGTGACAACT ATTACAGGAC TGTCTACTTC AGCAATAGCC 
    ACAAATGGGT TTGTAAGAGG AGGAGGTGCA TATTACTTAA TTTCCAGAAG TCTGGGACCG 
    GAGTTTGGTG GTGCCATAGG TCTGATTTTT GCATTTGCCA ATGCTGTTGC AGTAGCAATG 
    TATGTTGTTG GCTTTGCAGA GACAGTTGTG GAGCTGCTCA AGGAAAATGG AACGTTGATG 
    GTGGATGAAA TGAATGACAT CAGAATCATA GGGGCTATCA CAGTGGTGGT ATTGCTGGGT 
    ATTTCTGTTG CCGGAATGGA ATGGGAGGCA AAAGCCCAGA TAGTCTTACT GGTGATCCTC 
    ATACTTGCTA TTGGAGACTT CATCATAGGC ACATTTATTC CTCTGGATAG CAAGAAGCCT 
    AAAGGTTTCT TTGGTTATAA AGCTGAAATA TTTATGGAGA ATTTTGGGCC AGATTTCCGA 
    GACGAGGAAA CTTTCTTTTC TGTATTTGCA ATTTTCTTCC CTGCTGCAAC TGGCATACTT 
    GCTGGTGCAA ATATCTCAGG TGATCTTGCG GATCCTCAGT CAGCCATACC TAAAGGAACG 
    TTGTTGGCCA TCTTAATTAC TACATTGGTT TACGCAGGAA TTGCAGTATC TGTAGGTTCC 
    TGTGTTGTTC GAGATGCCAC AGGAAACATT AATAACACCA TTGTCACTGA GCTGACAAAT 
    TGCACTACTG CAGCTTGCAA ATTAAACTAT GATTTCTCAT CCTGTGAGAC AGGGTGTCAC 
    TATGGGCTGA TGAACAATTT CCAGGTAATG AGCATGGTAT CGGGATTTGC ACCATTGATT 
    TCTGCAGGTA TTTTTTCAGC CACCCTGTCT TCTGCATTAG CATCTCTTGT GAGTGCACCT 
    AAGATCTTCC AGGCTTTGTG CAAGGACAAC ATTTATCCTG CATTTCAGAT GTTTGGTAAA 
    GGCTATGGGA AGAACAATGA ACCACTGAGA GGATATCTTC TAACGTTTTT AATTGCTCTT 
    GGATTCATAC TAATTGCTGA ACTGAACGTC ATTGCTCCAA TTATTTCTAA CTTCTTCTTG 
    GCCTCATATG CCTTGATTAA CTTCTCTGTA TTCCATGCTT CTCTTGCAAA ATCTCCAGGT 
    TGGCGCCCTG CATTCAAATA CTACAATATG TGGATTTCTC TTGTTGGAGC AATTCTGTGC 
    TGCATTGTAA TGTTTGTCAT TAATTGGTGG GCAGCACTTC TAACATATGT CATAGTCCTT 
    GGACTCTACA TTTATGTCAC CTACAAGAAA CCAGATGTGA ACTGGGGATC CTCAACACAA 
    GCTCTGACTT ACTTGAATGC ACTGCAGCAT TCTATTCGGC TCTCAGGAGT GGAAGATCAC 
    GTGAAAAACT TTAGACCTCA GTGCTTAATT ATGACAGGGC CTCCAAATGC ACGTCCAGCT 
    TTGCTTCACC TTGTCCATGC TTTCACCAAG AATGTGGGCT TGATGATCTG TGGCCATGTA 
    CATATGGGTC CTCGGAGGCA AGCTATGAAG GAACTTTCAA CTGATTTGGC TAAATATCAG 
    CGATGGCTGA TTAAAAACAA GATGAAGGCT TTCTATGCAC CAGTGCATGC AGAGGACTTG 
    AGGGATGGAG GACAATACTT AATGCAGGCT GCTGGTCTGG GTAGAATGAG ACCTAATACT 
    CTTGTTGTTG GATTTAAGAA AAACTGGAGA CAAAGTGACA TGAGAGATGT TGAAACCTAT 
    ATCAATTTAT TCCATGATGC CTTTGATGTT CAGTATGGCG TAGTTGTAAT CCGCCTAAAG 
    GAAGGCCTGG ACATTTCTCA CCTTCAGGGC CAAGAAGAGT TACTGTCATC CCAAGAAAAA 
    TCTCCGTGTA CCAAGGACGT CATAGTAAAA GTGGATTATA GCAAGAAGTC TGAGACAGAC 
    ACTTCTATAG TTTTTGCTCC ATCATCTAGT GAAAGAACTC CCACTCCACA TAAAGATTCA 
    AAGAGCCCAT CTTCTCCTTT AAACTTGGCT GACCAAAGAC TTCTTGATGC TAGTACTCAG 
    TTTCAGAAGA AACAAGGCAA AAGCAATATT GATGTCTGGT GGCTCTTTGA TGATGGAGGT 
    TTGACACTGT TGATTCCGTA TCTTATTACA ACCAAGAAGA AGTGGAAAGA CTGTAAGATC 
    AGAGTGTTCA TTGGTGGAAA AATAAACAGG ATTGATCATG ACAGAAGAGC GATGGCTACT 
    TTGCTGAGCA AATTTCGGAT AGACTTCTCG GACATTATGG TTCTTGGGGA TATTAATACT 
    AAGCCAAAGA AAGAAAATAT TGCAGCTTTT GAAGAAATGA TAGAGCCCTT CCGACTTCAT 
    GAAGATGATA AAGAACAAGA AATTGCAGAT AAAATGAAGG AGGATGAACC ATGGAGGATA 
    ACAGATAACG AACTTGAGCT TTACAAAACA AAGACTTACC GCCAGATCAG GCTAAATGAG 
    TTGCTGAAAG AACATTCAAG TACAGCTAAC ATAATTGTCA TGAGTTTGCC AGTTGCACGA 
    AAAGGTGCCG TTTCTAGCGC GCTGTACATG GCCTGGTTAG AAGCTCTTTC TAAAGATCTG 
    CCACCAATTC TCCTTGTTCG TGGGAATCAT CAGAGCGTTC TTACCTTCTA TTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014810287.1
    CDS54..3470
    Translation

    Target ORF information:

    RefSeq Version XM_014954801.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014954801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAGGGCA AGCAGCAGCA GCCGCAGCCG GAGGAGCTGC CTCCTCCCGG CCTCGCCGTC 
    CCGGCGGGCT CGGAGGAGGG CGGCAAGGAT GCGGCGGGCG GCGGTGCGCT GGGCAGACCG 
    CTGGGTCCCA CGCCCAGCCA GAGCCGCTTC CAGGTGGACC TGGTGGCCGA GGGCGCCGGC 
    CGGCCGCCCG AGGAATCCCG CAAGGCGAGC GGGGGCGGCG GCACGGCGGG GAAGGGCAGC 
    GAGGAAGCCA AGGGCAGGTT TCGGGTGAAC TTCGTGGACC CGTCGGCCGG TGACGAGAGC 
    CCCGGCGAGC CGGGGGGCGG CAGCTCGGAG GGCGGCAATG TCAGCTTCCA GAACGGCGGC 
    GACACGGTGC TGAGCGAGGG CAGCCTCCAC TCCGGCGGGC ACCACCACTA CTACTATGAC 
    ACCCACACCA ACACCTACTA CCTGCGCACC TTCGGCCACA ACACCATGGA CGCCGTGCCC 
    CGCATCGACC ACTACCGCCA CACGGCCGCC GACCTGGGCG AGAAGCTCAT CCGGCCCAGC 
    CTGGCCGAGC TGCACGATGA GCTGGACAAG GAGCCCTTTG AAGATGGCTA TGCAAATGGT 
    GAGGAAGGCA CACCAGCTGG AGAAGCAGCT GCCACTTACA CACCTGATAG CAAAGGAGTG 
    GTCAAGTTTG GCTGGATAAA AGGCGTATTG GTACGATGTA TGTTAAATAT TTGGGGTGTG 
    ATGCTCTTCA TCAGACTCTC ATGGATTGTA GGACAAGCTG GGATAGGTCT GTCTGTTGTG 
    GTTATTGGAA TGGCCACTGT TGTGACAACT ATTACAGGAC TGTCTACTTC AGCAATAGCC 
    ACAAATGGGT TTGTAAGAGG AGGAGGTGCA TATTACTTAA TTTCCAGAAG TCTGGGACCG 
    GAGTTTGGTG GTGCCATAGG TCTGATTTTT GCATTTGCCA ATGCTGTTGC AGTAGCAATG 
    TATGTTGTTG GCTTTGCAGA GACAGTTGTG GAGCTGCTCA AGGAAAATGG AACGTTGATG 
    GTGGATGAAA TGAATGACAT CAGAATCATA GGGGCTATCA CAGTGGTGGT ATTGCTGGGT 
    ATTTCTGTTG CCGGAATGGA ATGGGAGGCA AAAGCCCAGA TAGTCTTACT GGTGATCCTC 
    ATACTTGCTA TTGGAGACTT CATCATAGGC ACATTTATTC CTCTGGATAG CAAGAAGCCT 
    AAAGGTTTCT TTGGTTATAA AGCTGAAATA TTTATGGAGA ATTTTGGGCC AGATTTCCGA 
    GACGAGGAAA CTTTCTTTTC TGTATTTGCA ATTTTCTTCC CTGCTGCAAC TGGCATACTT 
    GCTGGTGCAA ATATCTCAGG TGATCTTGCG GATCCTCAGT CAGCCATACC TAAAGGAACG 
    TTGTTGGCCA TCTTAATTAC TACATTGGTT TACGCAGGAA TTGCAGTATC TGTAGGTTCC 
    TGTGTTGTTC GAGATGCCAC AGGAAACATT AATAACACCA TTGTCACTGA GCTGACAAAT 
    TGCACTACTG CAGCTTGCAA ATTAAACTAT GATTTCTCAT CCTGTGAGAC AGGGTGTCAC 
    TATGGGCTGA TGAACAATTT CCAGGTAATG AGCATGGTAT CGGGATTTGC ACCATTGATT 
    TCTGCAGGTA TTTTTTCAGC CACCCTGTCT TCTGCATTAG CATCTCTTGT GAGTGCACCT 
    AAGATCTTCC AGGCTTTGTG CAAGGACAAC ATTTATCCTG CATTTCAGAT GTTTGGTAAA 
    GGCTATGGGA AGAACAATGA ACCACTGAGA GGATATCTTC TAACGTTTTT AATTGCTCTT 
    GGATTCATAC TAATTGCTGA ACTGAACGTC ATTGCTCCAA TTATTTCTAA CTTCTTCTTG 
    GCCTCATATG CCTTGATTAA CTTCTCTGTA TTCCATGCTT CTCTTGCAAA ATCTCCAGGT 
    TGGCGCCCTG CATTCAAATA CTACAATATG TGGATTTCTC TTGTTGGAGC AATTCTGTGC 
    TGCATTGTAA TGTTTGTCAT TAATTGGTGG GCAGCACTTC TAACATATGT CATAGTCCTT 
    GGACTCTACA TTTATGTCAC CTACAAGAAA CCAGATGTGA ACTGGGGATC CTCAACACAA 
    GCTCTGACTT ACTTGAATGC ACTGCAGCAT TCTATTCGGC TCTCAGGAGT GGAAGATCAC 
    GTGAAAAACT TTAGACCTCA GTGCTTAATT ATGACAGGGC CTCCAAATGC ACGTCCAGCT 
    TTGCTTCACC TTGTCCATGC TTTCACCAAG AATGTGGGCT TGATGATCTG TGGCCATGTA 
    CATATGGGTC CTCGGAGGCA AGCTATGAAG GAACTTTCAA CTGATTTGGC TAAATATCAG 
    CGATGGCTGA TTAAAAACAA GATGAAGGCT TTCTATGCAC CAGTGCATGC AGAGGACTTG 
    AGGGATGGAG GACAATACTT AATGCAGGCT GCTGGTCTGG GTAGAATGAG ACCTAATACT 
    CTTGTTGTTG GATTTAAGAA AAACTGGAGA CAAAGTGACA TGAGAGATGT TGAAACCTAT 
    ATCAATTTAT TCCATGATGC CTTTGATGTT CAGTATGGCG TAGTTGTAAT CCGCCTAAAG 
    GAAGGCCTGG ACATTTCTCA CCTTCAGGGC CAAGAAGAGT TACTGTCATC CCAAGAAAAA 
    TCTCCGTGTA CCAAGGACGT CATAGTAAAA GTGGATTATA GCAAGAAGTC TGAGACAGAC 
    ACTTCTATAG TTTTTGCTCC ATCATCTAGT GAAAGAACTC CCACTCCACA TAAAGATTCA 
    AAGAGCCCAT CTTCTCCTTT AAACTTGGCT GACCAAAGAC TTCTTGATGC TAGTACTCAG 
    TTTCAGAAGA AACAAGGCAA AAGCAATATT GATGTCTGGT GGCTCTTTGA TGATGGAGGT 
    TTGACACTGT TGATTCCGTA TCTTATTACA ACCAAGAAGA AGTGGAAAGA CTGTAAGATC 
    AGAGTGTTCA TTGGTGGAAA AATAAACAGG ATTGATCATG ACAGAAGAGC GATGGCTACT 
    TTGCTGAGCA AATTTCGGAT AGACTTCTCG GACATTATGG TTCTTGGGGA TATTAATACT 
    AAGCCAAAGA AAGAAAATAT TGCAGCTTTT GAAGAAATGA TAGAGCCCTT CCGACTTCAT 
    GAAGATGATA AAGAACAAGA AATTGCAGAT AAAATGAAGG AGGATGAACC ATGGAGGATA 
    ACAGATAACG AACTTGAGCT TTACAAAACA AAGACTTACC GCCAGATCAG GCTAAATGAG 
    TTGCTGAAAG AACATTCAAG TACAGCTAAC ATAATTGTCA TGAGTTTGCC AGTTGCACGA 
    AAAGGTGCCG TTTCTAGCGC GCTGTACATG GCCTGGTTAG AAGCTCTTTC TAAAGATCTG 
    CCACCAATTC TCCTTGTTCG TGGGAATCAT CAGAGCGTTC TTACCTTCTA TTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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