PREP cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	PREP
Entrez Gene ID	106901592
Full Name	prolyl endopeptidase
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014964437.1	XP_014819923.1	prolyl endopeptidase

Pathways from BioSystems
Gene Ontology

The following PREP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PREP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa81196	XM_014964437.1 Latest version!	Calidris pugnax prolyl endopeptidase (PREP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PREP ( XM_014964437.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014819923.1 Calidris pugnax prolyl endopeptidase (PREP), mRNA.

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CloneID	OCa81196
Clone ID Related Accession (Same CDS sequence)	XM_014964437.1
Accession Version	XM_014964437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	prolyl endopeptidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094507.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 18% of CDS bases ##RefSeq-Attributes-END##

ATGGCCTCCT TGGCCAATGT GGCCTCCACT TCTCGGGTGA AGGGGAGTAT CTGGCCGATT 
GTGGAGAAGC AGGGTGAAAC CCCTTGGCCG GCTGGTGAGG GGGAAACAAC CCCTCCGCTG 
GAGCATGGGG CGAGCGGAGG CGCCTGCACC CCGGGCCCCG GCCCCGTCCA GCCGGCGCCT 
GTGCCGGCGG CTCCCTGCAG GGGGCGCTGT GGGGCTCTGC CCGCCGCCGC CGGCCCGCTC 
TTGGCTGGGG GCGGGTCTTA TGGCGGCGCC GCTCCGCCTG GGCCGCGCCG CACGCCTGCT 
GCCGGCTGCC GCTACGGCCC TCGGTCTCGG GTTGTTCACG TCCGTGGGCC GCGGCGGCGG 
CTGGGGGCTT GTGCAAAGCC GGGTTTGTGG CCGCCGTGCC TGTGGGCAAG TCCCGGGTGT 
GGGGGGCTGC TGCCGGCCGG CTGGGGCGGG GGGCGGCCGG GGGAAGGAGC CGGTCTGGGC 
GCCCACCCCG GGGCTGCCGG TACCGCTGGG TGTCTGCGGG GAAGCCAGGC CTCGGCCGGG 
GCAGAGGAAC CAGCAGCCGT ATTTTTGTGT GGTGGCACAT CTGTAAGACC CCCTCCTTCT 
AGCCATGGAC GGGTGTTGGA TTACCATGGG TGTAAAATCA GTGATCCCTA CTGCTGGCTT 
GAAGATCCCG ACAGTGAACA GACGAAGGCG TTTGTGGAGG CTCAGAACAA GCTTACAGTA 
CCCTTTCTCG AGCAGTGTCC TGTCAGAGGG CTGTTCAAAG AACGAATGAC TGAACTCTAC 
GATTATCCTA AGTACAGTTG CCACTTCAAG AAAGGAAAAA GGTATTTTCA CTTCTACAAT 
ACAGGACTGC AGAACCAGCG AGTTTTATAT GTACAGGATT CCTTGGATGC CGATGCCAAA 
GTTTTTCTTG ATCCAAATAA GCTTTCTGAT GATGGCACTG TGGCTTTACG AGGTTATGCA 
TTCAGTGAAG ATGGTGAATA CTTTGCATAT GGCCTGAGTT CTAGTGGCTC TGATTGGGTC 
ACTATCAAGT TTATGAAAGT AGAAGGTCCT GAGGATCTTC CAGATACGCT GGAGAGAGTT 
AAATTCAGTT GCATGGCATG GACCCATGAT GGAAAAGGCA TGTTCTATAA TTGCTATCCA 
AAACAAGATG GGAAAAGTGA TGGTACTGAG ACCTCCACTA ATCTGCACCA AAAGCTGCAT 
TATCATGTGT TAGGAACTGA CCAGTCAGAA GATATCCTGT GTGCTGAATT TCCCGATGAA 
CCAAAATGGA TGGGTGGAGC TGAGGTCTCA GACGATGGTC GATACGTCTT GCTGTCTATT 
CGAGAAGGGT GTGATCCAGT GAACAGACTG TGGTACTGTG ATCTACAGAC AGAGTCTCAG 
GGTATATCAG GAATTCTTCA ATGGGTGAAG CTGATAGATA ACTTTGAGGC TGAGTATGAG 
TATGTCACCA ATGAAGGAAC AGTGTTCACT TTCAAGACAA ACCGCCACTC TCCAAATTAC 
CGGTTAATCA ACATTGACTT CAGTGACCCA GAGGAATCCA AATGGAAAGT TCTCGTCCCT 
GAACATGAAA GAGATGTTCT AGAGTGGGTT GCTTGTGTGA GGTCCAACTT TCTGGTTTTG 
TGCTACCTAC ACGACGTGAA GAACATCCTG CAACTACATG ACCTAGCTAC TGGTGCACAT 
CTCAAGACTT TCCCCCTAGA GGTTGGCAGT ATTGTGGGGT ATAGCGGCCA AAAGAAGGAC 
ACCGAAATAT TCTATCAGTT TACTTCCTTT TTATCTCCAG GTATTATATA TCACTGTGAT 
CTGACCAAAG AGGAACTGGA GCCAAGAGTT TTCCGGGAGG TGACTGTGAA AGGATTTGAT 
CCTTCGGTTT ACCAGACAAT TCAGGTTTTC TACCCGAGCA AAGATGGTAC TAAGATCCCA 
ATGTTTATTA TTCACAAGAA AGGAATTAAA TTGGATGGTT CTCATCCCGC CTTCTTGTAC 
GGATATGGAG GCTTCAACAT ATCAATTACA CCAAGTTACA GTGTGTCAAG ACTTATTTTT 
GTGCGACACC TTGGGGGTGT TCTAGCGGTG GCGAACATCA GGGGTGGTGG TGAATATGGA 
GAGACCTGGC ACAAAGGTGG TATCTTGGCC AACAAACAAA ATTGCTTTGA TGATTTTCAG 
TGTGCTGCCA AATACCTCAT CAAGGAAGGC TACACATCCC CAAAGAAGTT GACAATTAAT 
GGAGGCTCAA ATGGGGGCCT CTTAGTCGCT GCCTGTGCCA ATCAGCGCCC CGACCTCTTT 
GGTTGTGCCA TTGCCCAAGT GGGAGTGATG GACATGCTCA AGTTCCACAA GTACACCATC 
GGCCATGCTT GGACCACCGA CTACGGTTGC TCCGACTGTA AAGAGCAGTT TGAATGGCTG 
TGCAAGTACT CTCCACTTCA CAACATCAAA CTACCAGAAG AAGATGGCAT CCAGTATCCC 
TCTACGCTGC TTCTCACAGC AGACCATGAC GATCGCGTGG TCCCTCTCCA TTCGCTGAAG 
TTTATCGCTA CTCTGCAATA CGTTGTGGGC CGAAGCCGTA AACAAACCAA TCCGCTTCTC 
ATCCACGTCG ACACCAAGGC TGGGCACGGA GCAGGAAAAC CAACTGCTAA AGTTATAGAA 
GAAGTGTCAG ATATGTTTGC TTTTATAGCA CGATGCCTAA ATCTTGAGTG GATTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014819923.1
CDS	1..2700
Translation	MASLANVASTSRVKGSIWPIVEKQGETPWPAGEGETTPPLEHGASGGACTPGPGPVQPAPVPAAPCRGRCGALPAAAGPLLAGGGSYGGAAPPGPRRTPAAGCRYGPRSRVVHVRGPRRRLGACAKPGLWPPCLWASPGCGGLLPAGWGGGRPGEGAGLGAHPGAAGTAGCLRGSQASAGAEEPAAVFLCGGTSVRPPPSSHGRVLDYHGCKISDPYCWLEDPDSEQTKAFVEAQNKLTVPFLEQCPVRGLFKERMTELYDYPKYSCHFKKGKRYFHFYNTGLQNQRVLYVQDSLDADAKVFLDPNKLSDDGTVALRGYAFSEDGEYFAYGLSSSGSDWVTIKFMKVEGPEDLPDTLERVKFSCMAWTHDGKGMFYNCYPKQDGKSDGTETSTNLHQKLHYHVLGTDQSEDILCAEFPDEPKWMGGAEVSDDGRYVLLSIREGCDPVNRLWYCDLQTESQGISGILQWVKLIDNFEAEYEYVTNEGTVFTFKTNRHSPNYRLINIDFSDPEESKWKVLVPEHERDVLEWVACVRSNFLVLCYLHDVKNILQLHDLATGAHLKTFPLEVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGFDPSVYQTIQVFYPSKDGTKIPMFIIHKKGIKLDGSHPAFLYGYGGFNISITPSYSVSRLIFVRHLGGVLAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAKYLIKEGYTSPKKLTINGGSNGGLLVAACANQRPDLFGCAIAQVGVMDMLKFHKYTIGHAWTTDYGCSDCKEQFEWLCKYSPLHNIKLPEEDGIQYPSTLLLTADHDDRVVPLHSLKFIATLQYVVGRSRKQTNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNLEWIE

Target ORF information:

RefSeq Version	XM_014964437.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax prolyl endopeptidase (PREP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014964437.1

ORF Insert Sequence:

ATGGCCTCCT TGGCCAATGT GGCCTCCACT TCTCGGGTGA AGGGGAGTAT CTGGCCGATT 
GTGGAGAAGC AGGGTGAAAC CCCTTGGCCG GCTGGTGAGG GGGAAACAAC CCCTCCGCTG 
GAGCATGGGG CGAGCGGAGG CGCCTGCACC CCGGGCCCCG GCCCCGTCCA GCCGGCGCCT 
GTGCCGGCGG CTCCCTGCAG GGGGCGCTGT GGGGCTCTGC CCGCCGCCGC CGGCCCGCTC 
TTGGCTGGGG GCGGGTCTTA TGGCGGCGCC GCTCCGCCTG GGCCGCGCCG CACGCCTGCT 
GCCGGCTGCC GCTACGGCCC TCGGTCTCGG GTTGTTCACG TCCGTGGGCC GCGGCGGCGG 
CTGGGGGCTT GTGCAAAGCC GGGTTTGTGG CCGCCGTGCC TGTGGGCAAG TCCCGGGTGT 
GGGGGGCTGC TGCCGGCCGG CTGGGGCGGG GGGCGGCCGG GGGAAGGAGC CGGTCTGGGC 
GCCCACCCCG GGGCTGCCGG TACCGCTGGG TGTCTGCGGG GAAGCCAGGC CTCGGCCGGG 
GCAGAGGAAC CAGCAGCCGT ATTTTTGTGT GGTGGCACAT CTGTAAGACC CCCTCCTTCT 
AGCCATGGAC GGGTGTTGGA TTACCATGGG TGTAAAATCA GTGATCCCTA CTGCTGGCTT 
GAAGATCCCG ACAGTGAACA GACGAAGGCG TTTGTGGAGG CTCAGAACAA GCTTACAGTA 
CCCTTTCTCG AGCAGTGTCC TGTCAGAGGG CTGTTCAAAG AACGAATGAC TGAACTCTAC 
GATTATCCTA AGTACAGTTG CCACTTCAAG AAAGGAAAAA GGTATTTTCA CTTCTACAAT 
ACAGGACTGC AGAACCAGCG AGTTTTATAT GTACAGGATT CCTTGGATGC CGATGCCAAA 
GTTTTTCTTG ATCCAAATAA GCTTTCTGAT GATGGCACTG TGGCTTTACG AGGTTATGCA 
TTCAGTGAAG ATGGTGAATA CTTTGCATAT GGCCTGAGTT CTAGTGGCTC TGATTGGGTC 
ACTATCAAGT TTATGAAAGT AGAAGGTCCT GAGGATCTTC CAGATACGCT GGAGAGAGTT 
AAATTCAGTT GCATGGCATG GACCCATGAT GGAAAAGGCA TGTTCTATAA TTGCTATCCA 
AAACAAGATG GGAAAAGTGA TGGTACTGAG ACCTCCACTA ATCTGCACCA AAAGCTGCAT 
TATCATGTGT TAGGAACTGA CCAGTCAGAA GATATCCTGT GTGCTGAATT TCCCGATGAA 
CCAAAATGGA TGGGTGGAGC TGAGGTCTCA GACGATGGTC GATACGTCTT GCTGTCTATT 
CGAGAAGGGT GTGATCCAGT GAACAGACTG TGGTACTGTG ATCTACAGAC AGAGTCTCAG 
GGTATATCAG GAATTCTTCA ATGGGTGAAG CTGATAGATA ACTTTGAGGC TGAGTATGAG 
TATGTCACCA ATGAAGGAAC AGTGTTCACT TTCAAGACAA ACCGCCACTC TCCAAATTAC 
CGGTTAATCA ACATTGACTT CAGTGACCCA GAGGAATCCA AATGGAAAGT TCTCGTCCCT 
GAACATGAAA GAGATGTTCT AGAGTGGGTT GCTTGTGTGA GGTCCAACTT TCTGGTTTTG 
TGCTACCTAC ACGACGTGAA GAACATCCTG CAACTACATG ACCTAGCTAC TGGTGCACAT 
CTCAAGACTT TCCCCCTAGA GGTTGGCAGT ATTGTGGGGT ATAGCGGCCA AAAGAAGGAC 
ACCGAAATAT TCTATCAGTT TACTTCCTTT TTATCTCCAG GTATTATATA TCACTGTGAT 
CTGACCAAAG AGGAACTGGA GCCAAGAGTT TTCCGGGAGG TGACTGTGAA AGGATTTGAT 
CCTTCGGTTT ACCAGACAAT TCAGGTTTTC TACCCGAGCA AAGATGGTAC TAAGATCCCA 
ATGTTTATTA TTCACAAGAA AGGAATTAAA TTGGATGGTT CTCATCCCGC CTTCTTGTAC 
GGATATGGAG GCTTCAACAT ATCAATTACA CCAAGTTACA GTGTGTCAAG ACTTATTTTT 
GTGCGACACC TTGGGGGTGT TCTAGCGGTG GCGAACATCA GGGGTGGTGG TGAATATGGA 
GAGACCTGGC ACAAAGGTGG TATCTTGGCC AACAAACAAA ATTGCTTTGA TGATTTTCAG 
TGTGCTGCCA AATACCTCAT CAAGGAAGGC TACACATCCC CAAAGAAGTT GACAATTAAT 
GGAGGCTCAA ATGGGGGCCT CTTAGTCGCT GCCTGTGCCA ATCAGCGCCC CGACCTCTTT 
GGTTGTGCCA TTGCCCAAGT GGGAGTGATG GACATGCTCA AGTTCCACAA GTACACCATC 
GGCCATGCTT GGACCACCGA CTACGGTTGC TCCGACTGTA AAGAGCAGTT TGAATGGCTG 
TGCAAGTACT CTCCACTTCA CAACATCAAA CTACCAGAAG AAGATGGCAT CCAGTATCCC 
TCTACGCTGC TTCTCACAGC AGACCATGAC GATCGCGTGG TCCCTCTCCA TTCGCTGAAG 
TTTATCGCTA CTCTGCAATA CGTTGTGGGC CGAAGCCGTA AACAAACCAA TCCGCTTCTC 
ATCCACGTCG ACACCAAGGC TGGGCACGGA GCAGGAAAAC CAACTGCTAA AGTTATAGAA 
GAAGTGTCAG ATATGTTTGC TTTTATAGCA CGATGCCTAA ATCTTGAGTG GATTGAATAG