SACS cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	SACS
Entrez Gene ID	106902433
Full Name	sacsin molecular chaperone
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014965841.1	XP_014821327.1	sacsin isoform X1
XM_014965842.1	XP_014821328.1	sacsin isoform X2

Pathways from BioSystems
Gene Ontology

The following SACS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SACS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa82206	XM_014965841.1 Latest version!	Calidris pugnax sacsin molecular chaperone (SACS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa82207	XM_014965842.1 Latest version!	Calidris pugnax sacsin molecular chaperone (SACS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SACS ( XM_014965841.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821327.1 Calidris pugnax sacsin molecular chaperone (SACS), transcript variant X1, mRNA.

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CloneID	OCa82206
Clone ID Related Accession (Same CDS sequence)	XM_014965841.1
Accession Version	XM_014965841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094510.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAT ATCAGGGGCC AGCATTTTAT GCATACAACG ATGCAATTTT CACCCCTGAG 
GACTGGCATG GTATACAGGA AATAGCAAGA AGCAGGAAGA AAGATGATCC CCTGAAAGTT 
GGAAGATTTG GAATTGGCTT TAATTCAGTT TATCATATAA CAGATGTCCC TAGCATTTTC 
AGTGGTGACC AGATTGGAAT GCTTGATCCC CATCAGACCC TTTTTGGACC TCATGAATCA 
GGCCAGTGCT GGAATCTAAA AGAAGATAGC AAGGAAATCA ATGAACTTAC AGACCAGTTT 
GCTCCGTTCA TTGGTGTGTT TGGCAGCACT AAGGAAACGT TTAAGAATGG AAGCTTTCCT 
GGTACCTTTT TTCGTTTCCC ACTTCGTCTG CAGCCTTCCC AGCTGAGCAG CAATGTTTAC 
GACAAACAGA AGGTTCTGGA GCTGTTTGAA TCCTTCAGAG CAGATGCAGA CACAGTATTG 
CTCTTTCTGA AGAGTGTTCA GGATGTGTCA TTGCATGTTA GGGAAGCTGA TGGAACTGAG 
AGGCTGATTT TTAGAGTAAC TGCTAGTGAG AACAAGGCCT TGAAACATGA AAGACCAAAT 
TCTATCAAGA TCCTAGGAAC TGCAATAAAT CAGTATTGTA AAGGAGTTCC AAGCAATAGC 
ATAACATGTG TGACATACCA TGTAAATATA GTTGTAGAAG ATGAGAGCGT TAAGGACGCA 
CAGAAAACAT CTTGGTTGGT GTGTAATTGT GTGGGTGGTC GAGGAATGTG TACTGAACTG 
GATAGTTTAG CTGATGACTT GAAATTTGTT CCTACTATTG GAATAGCCAT GTCTTTATCA 
ACTAATGAGG AGGAAAAAGG AGCTGTAGCA GACTTTTCAG GAAGAGCGTT TTGTTTTCTG 
CCTTTGCCTC CAGGTGAAGA GAGCAAAACT GGACTCCCAG TTCATGTCAG TGGTTTCTTT 
GGTCTCACGG ACAATAGGAG GAGTATCAAA TGGCGAGAGC TGGATCAATG GAGAGATCCA 
GCAGCTTTGT GGAATGATCT TCTCGTCGTG AATATTGTGC CCAAGGCATA CACCACCCTT 
ATTTTGGAAG CTATCAAACG AATGGAGACT GAGAAGAATT CAGATTTTCC ATTGTCAGCT 
GAACGAATTT ATAGGTTATG GCCTGATGAG AACAAAATCA GGGTACCTTG GAAACCAATT 
GTAGTACCTC TCTTTAAAGA GCTGCTCCAG CATACAGTTA TTTACTCAGT GAGTAATCAG 
TGGATACAGG TTGAACAGGT GCACTTTTCT GAAATGGATG AGAGTTTGGA GTACACACAG 
TCTGTTCTCA ACTACCTCCA GAATTCAGGG AAGCAGATTG CCAAGGTACC AGCAAATATT 
GCTAGTGCAG TGCATCTTAC TATTTCTGCT GCTAAAGCTG TGAAAAAAGT GACTCCTGCT 
GTAGTACGGC AAGTACTAAG GAAATCTGGA CACAGTGGAA CTGCTGAAGA GAAGCTTCAT 
CTTCTAGAAT TTGTGCTTTC TGATGGAGTC TACAGTGAAC TGATTGGACT GGAGCTTTTG 
CCATTACAAA ATGGAAATTT TATTCCTTTT TCCTCATCTG TGTCAGAACA GGATGTAGTT 
TACATAACCT CAGAAGAATA TCCCAGATCA CTCTTTCCTG GCCTGGAAGG AAGGTTACTT 
TCTGATGATC TGAAACCTGA GGTTCTAGCT GCCTTGAAAG AAGCTGCCAA AAGCCGAGCC 
CAACAGCTTC TCTGGGGATT TTCAGGAAGG CCATGCACTC AGCTGCAGCT TCTGAACCCA 
GAAAGATTCG CTCGGCTCAT TAAAGAAGTT ATGAATAGCA CGTGGCCTGG CAGAGACATG 
GTGGTACAGT GGCATCCAGG TTTGGAAGAC AAAAATCACC CATCAGTTTC CTGGCTTAAG 
ATGGTCTGGA AGAACCTATA TATACATTTT TCTGATGACT TGAGTGTGTT TGATGACATG 
CCGCTTATTC CCAAGACACT GCTGGAGGAA AACCAAACAT CAGTGGAACT GGTTAGACTT 
AGGAATCCAT CACCTATCAT TCTGGAAGAT GAATCTGAGA CTCAGCTCCC GGAATATTTA 
GCTGATATTA TTCAGAAAGT TGGTGGAGTT GTACTTAAGC AGCTAGATAT TTCTATCCAA 
CATCCGCTTA TAAAAAAATA CGTGCATCCA CCATTACCAA GTGCTGTCTT ACAAATCATG 
GAAAAAATGT CCTTACAGAA ACTATGCAGT CAGGTTGCAT CATTCCCGTC AACACATAAA 
GATGCTCTTA GGGCATTCTT GGCTAGTTTA ACTGATGTAA GTGAAAAAGA GAGAAGAATT 
ATTCAGGAAT TGCTAATATT TAAAAAAATT GAAAAATCAT CTGATGAAAG TGTCCCTGTT 
TTTACTGGGC TAAAAGGTAG TAAAGTATTA CACCACACTG CCAAAATTCC TCCTGGTCTA 
AGATTTTCTG TTCCAATAAT TGACAGCAGT GATGAAGCTA CTATTCGCTT AGCTAACCTG 
CTAAAAATAG AACAGCTAAA GAGCACAGAT TGCTTGAAGT TTGTTATACA GGACATAAGA 
AGTGATTTTT ATTCATATGA TGAAATAACA CAGATAATGC AATGGGTTCT TGAAAATCTG 
ACTTTTCTGA AAAATGAGAA TACAGATGTG ATAGATTGGC TGACATCACT AAAGTTTATT 
AAGATTTCAG AGGAAAATAT TATGTCAGCA AGCGAACTCT TTGATCCTGA GGTAGAAGTT 
CTGCAAAACT TATTTTATGC TGAGGAGGAA ATTTGCTTCC CTCCTGTTGT CTTTACATCT 
TCAGATATTC TTCATTCTCT GAGACAAATA GGCTTAAAAA ATGAGGCCAA TCTTGAGGAG 
AGTGATATTA TGAGAGTGGC AAATAAAATT GAAAGTTTGC ATGCTAACTC TAACACTGAT 
CATGATCTAC TGTTAAGAAA AGCTAGAACT CTCGTGATGG TTTTGAACAA AAACCATACA 
CTGCTTCACT CATCTGAAAC AAAAGCTGCA CTGAAAAAAA TAAAATGGAT CCCGGCATGT 
AAGGAAAGAC CTCCCAATTA TCCAGGGTCC TTAGTTTGGA AAGGAGACCA TTGCAATCTG 
TGTTCACCAC CAGAAATGTG TGATATAGCT CATGCAATTT TAGTTGGTTC CTCAGTTCCT 
CTTGTGGAAA ATGTGCTGTT GGATACAGAA AAAGCCCTCG GCATCCCCAC CAAGCCCAGC 
ATAAAAGCAG TCTTAAAACA TTTCAAGGTG GTGGTTGATT GGCACAGTTC TAAAACCTTC 
AGTGATGAGG ACTACTATCA GTTTCAGCAT ATCTTGCTTG AGATTTATGG ATTTATGCAT 
GATCATCTAG AAGAAGGCAA AGAAGCTTTT AAAGCCCTGA AATTTCCCTG GGTTTGGACA 
GGTAAAACAT TTTGTTCCCT CACACAAGCA GTAATAAAGT CAGTACCTGA TCTCGATCTC 
CAACCTTATC TACATTATGT GCCAAAAACG ATGGCGAAGT TTCACCAGTT GTTTATATGT 
TGTGGTTCAA TAGAGCAGTT AACACCAGAC CACGTGTCTA TGGTCATTCA GAAAATATAT 
CTGAAAAGCG AGCAGCCTCT CTCGGAACAA GAGAGTAAAC AAAATCTTCA CATTATGTTG 
AGCATCATAA GGTGGCTGTA CAGCAATCAG ATTCCTGCAA GTCTGCACAC GCCCATGCCG 
CTCTACAGTG GCAAACTTCC CTACAAACTT GCCATGAGGC CCATTCACGA GTGCTGTTAC 
TGCGACATCA AAGTGGATGA CTTAAACGAT TTGCTGGAAG ATTCTGTTGA ACCGATAATT 
TTAGTGCATG AAGACATACC GATGAAAACC GCTGAGTGGT TAAATGTCCC ATGCCTTAGC 
ACAAGACTGA TTAATCCAGA AAATATGGGA TTTGAGCAGT CTGGGCAAAG AGAGCCTCTG 
ACTGTAAGAA TTAAAAACAT TTTGGAGGAA TACCCTTCCA TTTCAGATAT CTTTAAAGAG 
CTTCTTCAAA ATGCTGATGA TGCTAATGCA ACGGAATGCA ATTTCCTGAT TGACATGAGA 
AGAAATATGG ATATAAGAGA GAATCTTCTG GATCCAGGAA TGGCAGCATG TCATGGTCCA 
GCTTTGTGGT CATTCAATAA TTCTGAATTT TCTGACTCTG ATTTCCTAAA TATAACTCGA 
TTAGGAGAGT CTTTAAAAAG GAGTGAAGTT GACAAAGTAG GAAAATTTGG ACTGGGATTC 
AATTCTGTCT ATCATATCAC TGATGTTCCT ATTATTTTGA GCCGGGAGTT CATGATTATG 
TTCGATCCAA ACGTTAACCA TATCAGTAAG CATATTAGAG ATAAATCTAA CCCTGGCATC 
AAAATCAACT GGAGTAAACA ACAGAAGAGG CTGCGAAAGT TTCCCAACCA GTTTAAGCCT 
TTTATAGGAG TTTTTGGTTG CCAGTTGCCA CTGACTGTAG AATCTCCTTA CAGCTACAAA 
GGAACTCTTT TCAGGCTGTC CTTTAGAACT CAGCAGGAAG CTAAAGTGAG TGAAGTCAGC 
AGTGCGTCTT ACAATACAGC AGACATTTAC TCTCTTGTGG ATGAGTTTAG CATCTGTGGT 
CATAGACTGA TAATATTTGC TCAGAGTGTA AATTCAATGG TTTTGAAATA CTTGAAAATT 
GAAGCAGATG ATCCTGGGGT GGCACAGGAT GTTGTCACTA TCAAAAAAAA TCTGTGTTCT 
TCCAAAGCCT TGACTGCCCC AAACGTAAGT GTTTTAAAGG AAGCGGCTAA ACTCATGAAG 
GTCTGCAATG GCAGTAGTAA AAAACTTCCC ACTGAAACAC CAAAGTCCTC GTGTATCTTA 
CAGATAGTAG TAGAAGAATT TCACCATGTA TTTAGACGGA TTGCTGATTT ACAGTCTCCA 
CTTTTCAGAG GTACAGAAGA TGATCCAGCT TCTTTCTTTG AAATGGCTAA ATCAGGACAG 
ATGAAGAGAT CAGCTGATGA ATTGCCACAG AAAACAGTAG ATTCAACAAC ATGGCTTATA 
TGTTCTTGCA TGGACACTGG AGATGCTTTA AAATTTTCAT TACATGAAAG TGGACGAAGA 
CTAGGTCTTG TTCCCTGTGG TGGAGTAGGA GTGTTGCTCT CTGAAACTCA GGATCAAAAA 
TGGATTGTGA AACCTAATTG CAACAACATA GGTGAAGTAT TCTGCTATTT ACCTCTACGA 
ATCAAAACAG GCTTACCTCT TCATATAAAT GGCTGCTTTG CAGTTACTTC AAATCGGAAA 
GAAATCTGGA AAACGGACAC AAAGGGAAGA TGGAATACAG TATTCATGAG GCATGTTATT 
GTAAAAGCCT ACATAGAAGC CCTTTGTGTT CTGCGTGACA TGGCAATCAA TGGTGAGCTT 
GTTGACTACA GTTACTCTGC AGTGTGGCCA GATCCTGATT CAGTTCATGA TGATTTTTCT 
GTTATTTGTC AAGGGTTTTA TGAAGACATA GCTCACATGA AGAACAAAGA AGGGATCAAA 
GTGTTTTCTG ATGGTTTCTC TTGGGTTTCC ATGAAGAATG TAAGGTTTTT AGATGACTCT 
ATACTGAAAC GACCAGATAT TGGACCATCA GCCTTTAAGA TCTTTCAGAA GTACCTTAAA 
AAAACTGGTT CTAAAAATCT GTGTGCTGTA GATCTTCCTT CCTGGGTAAA GACAGGATTT 
GAAGAAGCAG GATGCAAGTA TATATTACTG GAGAACACTT TTTCTGAGAA ACAGTTCTTT 
TCTGAGGTGT TTTTCCCTAA CATTCAGGAG ATTGATGCAG AGCTGCGTGA TCCTTTGATG 
CGTTATGTTC TCAATGAAAA AGTTGAGGAG TTCTCAGGAA TTCTTCGTGT TACTCCGTGT 
ATTCCCTGTT CGTTGGATGG ACATCCATTA GTTACACCAT CAAGACTGAT CCATCCTGAA 
GGAAGAGTTG CAAAATTATA TGATGCTGAG GATGGAAGAT TTCCTTACGG CACCACTCAG 
GATTATCTTA ACCCAGTTAT TTTGGTTAAA CTTGTTCAGT TGGGAATGGC TAAAGATGAT 
ATTTTATGGG AAGATCTGAT GGAACGTGCA GAGTCAGTAG CCGAAGTTAA CAAGATTGAT 
CATGCTGCAG CTTGTCTTAG AAGCAGCATT ATAGTGAGTC TTATTGATGA AAAACTAAAA 
TCTAGGGACC CTAGAGCTAA AGAATTTGCT GCAAAATGTC AAACCATCCC TTTCCTTCCT 
TTCCTTAGCA AACCAGCAGG CTTCTCACTG CACTGGAAAG GCAGTGATTT TCAGCCTGAA 
GCAATGTTTT CAGCAACTGA TCTTTTCACT GCTGATCATC AAGATACAGT TTGCCTAATA 
CAACCCATTC TTAATGAAAA TTCCCACTCC TTTAAAGGTT GTGGTGCTTT GTCATTAGCT 
GTCAAAGAAT TTTTGGGTTT ACTGAAGAAA CCAGCTGTTA ATTTGGTCAT AAATCAGTTA 
GAAGAAGTTG CAAAGTCATT TGATGGAATC ACGTTATATC AAGAAAATAT CACTAATGCT 
TGTTACAAAT ATCTACATGA AGCAATGTTA CAGAACGAAT CAACGAAAGC TATGATAATT 
GAACAACTGA CAAACTGTAG TTTTATTCTG GTTGAGAATG TGTATGTTGA TCCAACAAAA 
GTGTCTTTTC ATTTGAATTT TGAAGCAGCA CCCTACCTCT ATCAGTTGCC TAACAAATAT 
AAAAATAGTT TCCGTGAGCT GTTTGAAAGC GTGGGGGTAA GACAGGCCTT TACAGTTGAA 
GATTTTGCTG TAGTTCTGGA ATTAATAGAT CAGGAAAGAG GGAACAAACA ACTAACAGAA 
GACAACTTTC AGCTTTGCAG GAGAATAATT AGTGAAGGAA TATGGGGCCT AATTAGAGAG 
AAGAAGCAGG AATTTTGTGA GAAAAAGTAC GGTGAGATTT TGTTACCTGA CACTCGTCTT 
GCACTTCTGC CTGCGAAATC TTTGTGTTAC AATGACTGTC CATGGATTAA GGTTAAAGAC 
ACAACCGTTA AATATTGTCA TGGTGATATT CCAAGAGAAG TTGCAGTAAA GCTTGGAGCA 
ATCCCAAAAC GTCATAAAGC TTTAGAAAGA TATGCATCCA ATATCTGTTT TACTACCCTT 
GGAACAGAGT TTGGCCAGAA AGAAAAATTG ACAAGTAGAA TTAAAAGCAT TCTTAATGCC 
TACCCGTCAG AAAAAGAAAT GCTGAAAGAA CTCCTTCAGA ATGCAGATGA TGCAAAAGCT 
ACAGAGATCT GTTTTGTGTT TGATCCTAGG CAGCATCCAG CTGATAGAAT ATTTGATGAG 
AAATGGGCAC CACTTCAAGG ACCAGCACTG TGTGTTTACA ACAACCAGCC TTTTACAGAA 
GATGATATTC GAGGAATTCA GAACCTTGGA AAAGGTACAA AAGTAGGAAA TCCCTGTAAA 
ACTGGACAAT ATGGTATAGG TTTCAATTCT GTTTATCACA TTACTGATTG CCCTTCTTTC 
ATATCTGGCA ATGATATACT TTGTATTTTT GATCCTCATG CTAGATATGC ACCAGGTTCA 
ACATCAACAA GTCCTGGCCG CATGTTTAGA GATTTAGATG CAGATTTCAG AACACAGTTC 
TCAGATGTGC TGGACCTTTA CTTAGGAGAT CACTTTAAAC TGGATAATTG CACAATGTTT 
AGGTTTCCTC TTCGAAATGG AGAAATGGCA AAAGTATCAG AAATTTCCTC AGTTCCATGT 
TCGGATAGAA TGGTCCAAAA CCTATTAGAT AAGCTGCGTA CAGATGGAGC AGAACTCTTA 
ATGTTTTTGA ACCATATGGA AAAAATTTCT ATTTGTGAGA TAGAAAAAAC AACAGGAGCA 
CTGAATGTGT TGTATTCTGT GCAAGGCAAA ATCACTGATG GAGACAGACT GAAACGAAAG 
CAATTCCATG CATCTGTAAT TGACAGCGTA AGTAAAAAAA AGCAGCTAAG TGAAATACCC 
GTGCAACAGA TTACTTACAC AATGGATACT GAAGACTCTG AAGGAAATCT TACAACTTGG 
TTAATTTGTA ACAGGTCAGG CTTTTCTGCC ATGGAAAAGG TATCCAAAAG TGTGGTTTCA 
GCCCACAAGA ATGAGGACAT TACTCTTTTT CCACGTGGCG GGGTAGCAGC TTGCATTACG 
CATAATTACA AAAAGCCCCA CAGAGCGTTC TGTTTCTTAC CGTTATCGTT AGAAACTGGA 
TTACCCTTTC ACGTGAATGG ACACTTTGCC CTGGATTCTG CAAGAAGAAA TCTGTGGCGT 
GATGATAACG GTGTTGGAGT AAGAAGTGAC TGGAATAGTA GCCTAATGAC AGCGCTGATT 
GCACCGGCCT ACGTTGAACT GCTGATTCAG CTGAAGAAAC GGTATTTCCC AGGCACTGAT 
CCTACAGTGT CCGTGCTGCA AAACACACCT GTTCATGTTG TGAAAGACAC TTTAAAAAAA 
TTTTTATCCT TTTTTCCAGT TAATAGACTT GATATTCAGC CGGATTGGTA TTGCTTAGTG 
AAAGCAGTTT ACAGTTGCAT TTATGAAGAT CTGAAGCGTC TTTTACCTGT TATGCGAGCT 
CCAAATATCG ATGGTTCTGA TTTGCATTCT GCTGTTATCA TCACATGGGT TAATATGTCT 
ACAGTGAACA AAGGCAGGCC ATTCTTTGAC AATTTACTAC AAGATGAATT GCAACATCTC 
AAAAATACAG AATACAACAT CACAACGCGG AAGACAGTGG CAGAAAATGT CTACAGACTC 
AAGCATTTAC TCTTAGAAAT TGGATTTAAT TTGGTATATA ACTGTGATGA AACTGCAAAT 
CTTTACCACT GTCTGGTAGA TGCAGATATT CCTGTCACCT ATGTGACACC TGCTGATGTC 
CGTTCATTTT TGATGACATT TTCTTTTCCA GACTCTAATT GCCACATTGG AAAGTTACCC 
TGTCGTCTTC AGCAGACGAA TTTGAAACTT TTCCATAGTC TCAAACTTCT GGTTGACTAC 
TGTTTTAAAG ATGCAGAAGA AAATGAAATC CAGATTGAGG GCTTGCCACT CCTTATTACT 
CTTGACAATG TTTTGCAAAT TTTTGATTCA AAGCGACCAA AGTTCTTAAC AACATACCAT 
GAGCTGATTC CATCTCGCAA GGATCTCTTT ATGAACACGT TGTACTTGAG ATACAACAAT 
ATTTTACTGA GCAGTGAAGT AGCGAAAGTC TTTGATATCA AAAGTTTTGC TGAATTGTTA 
TCATCTGTGT TGCCGAGAGA ATATAAAACC AGAAATTGTA TGAAATGGAA GGAAAACTTT 
GCAAGTGAGT CTTGGCTTAA AAACGCCTGG CACTTTATTA GTGAGTCTAT AAATATAAAG 
GAAGAGCAAG AAGATACGCA AGCAAAATTC GATGATGTTG TTGAAACTTT GAAGGACTGG 
ACTTTGCTCC CAGGTGTAAG GTTTACTGTC TCAGCTAATC ACCTTGTTGT GCCAGAGTGT 
GACGTTCTGC TGCCACTCAG CATTATGCAT ATAGCTGTTT TTCCAAATGC TCAGAGTGAT 
AAAGTCTTCC ATGCTTTAAT GAAAACAGGC TGTATTCAGT TGGCATTGAA CAAAATTTGC 
TCCAAAGACA GTGCTTTAGT GCCTTTGTTG TCAGGATGTA CAGCAAATAT AGATAATCCT 
TCAAGCATTC TGAAAGCCAT ACAATATATG GTACAGACAT CAACTTTCAG GACTGAAAAG 
TTAGCAGAAA GTGACTTTGA GGCTCTCTTA ATGTACTTCA ATTGCAATTT ATGTCATTTG 
ACATCTCAGG ATGACATTAA AATTTTAAAG TCTCTTCCAT GTTATAAATC GATCAGTGGC 
CGATACATCA GCATTTCAAA ATACGGGACG TGTTACGTTC TCACGAAAAG CATCCCATCA 
GTAGAAGTAG ACAGATGGAC ACAGTCAACT TCATCAGCTT TTCTTGAAGA GAAGGTTCAT 
TTGACAGATT TGTACACTCT GCTTGGCTGT GTCTCTGTGG ATGATCTTGA AGTGTACTTG 
AAACATCTTT TGCCAAAAAT TGAGAGCCTG TCTTACGATG CAAAATTAGA ACACTTGATC 
TACCTAAAGA ATAGGCTAAC TAGCATTGAG GAATCTTCAG AAATGAAAGA ACAGCTGTTT 
GAAAAGCTTG AAAGCATTTT GATCATTCAT GATGCAACCA ATAGATTAAA GCCTGCAAAA 
TATTTTTATG ACAGGACTGT AAAAGTCTTT GAAGTTATGC TTCCTGAAAA GTTCTTTATA 
CCTCAGGATT TTTTTAAGAA ACTCGAGCAA CTTACAAAAC CAAAAAACCA AGCTGCATTC 
CTTCCATCAT GGGTAACTTT TCTACGACAT ATCGGATTGA AATTCATCAT TTCGCAGCAA 
CAGTTGCTAC AGTTTGCTAA GGAGATCAGC ATGCGAGCGA ATACAGAAAA CTGGTCTAAA 
GAAACACTAC AGAATACTGT CGATGTCCTC CTTCATCACA TATTTCAAGA AAGAACTGAC 
CTTTTGTCAG GAAATTTTCT GAAAGAATTG TCATTAATTC CTTTTCTGTG CCCTGAACGA 
GCTCCAGCAG AATTTGTTAG ATTTCATCCT CAGTATCAAG AAGTTAATGG GACACTTCCT 
CTTATACGAT TCAATGGGGC ACAGGTGAAT CCTAAATTCA AACAGACAGA TGTGTTGCAG 
TTGCTGTGGA CTTCATGTCC CATTCTTCCT GAGAAAGCAA CACCTTTAAG CATCAAGGAG 
CAAGAGGGAA GTAGTCTTGG TGCACAAGAA CAACTTGAGC AAGTTTTAAC TATGCTGAAT 
GTCAATTTGG ACCCTCCCCT TGACAAAGTT ATCAATAACT GCAGGAACAT ATGCAATATA 
ACGACTTTGG ATGAGGACAT GGTGAAAACT AGGGCAAAGG TCTTAAGAAG CATTTATGAA 
TTTCTTAGTA CAGAGAAAAG GGAATTCCGC TTTCAACTTC GAGGAGTTTC TTTTGTAATG 
GTGGAAGAAG GTTGGAAGCT TCTGAAGCCT GAAGAGGTGG TAATAAACCT TGAGTATGAA 
TCTGATTTTA AACCTTATCT TTACAAACTT CCTTTAGAAC TTGGTACTTT CCATCAGTTA 
TTTAAACATT TGGGTACTGA AGATATTATT TCAACAAAGC AGTATGTTGA AGTTCTAGGT 
CGCATATACA AGAGCTCGGA AGGAAAGCAA TTGGATCCTA ATGAAATGCG CACGGTTAAA 
AGAGTAGTTT CTGGCCTGTT CAAAAGTCTT CAGAATGATT CTGTCAAGGT TAGGAATGAC 
CTTGAGAATA TGAGGGATTT TGCCCTTTAC CTTCCGAGTC AAGATGGTAG GTTGGTAAAG 
TCTAGTATCC TAGTTTTTGA TGATGCACCT CACTACAAAA GCAGAATCCA AGGTAACATT 
GGTGTGCAAA TGCTTGTTGA TCTTAGCCAG TGTTACTTAG GTAAAGACCA TGGCTTTCAC 
ACTAAATTGA TTATGTTATT CCCTCAGAAA TTGAGGCCTC GCTTACTTAG CAGTATTCTC 
GAAGAACAGT TGGATGAAGA GTCTCCCAAA ATCTGTCAGT TTGGAGCATT ATGTTCTTTG 
CAGGGAAGGT TACAGTTACT GTTGTCTTCG GAACAATTCA TCACAGGACT CATTAGGATT 
ATGAAGCATG AAAATGACAA TGCTTTTTTA GCTAATGAGG AAAAAGCAGT AAGACTTTGC 
AAAGCTTTGC GAGAAGGCTT GAAAGTTTCC TGTTTTGAGA AGTTGCAAAC AACGTTACGA 
GTTAAAGGCT TTGCTCCTAT TCCCCACAGC AAAAGCGAAA CATTTGCTTT CCTGAAGAGA 
TACGGAAATG CAGTAATACT GCTTTACATA CAGCATTCTG ACAGCAAAGA CATAAATTTC 
CTGTTGGCAT TAGCCATGAC CTTAAAATCT GCAACTGACA ACCTGATTTC TGATACCTCA 
TATTTAATTG CCATGCTGGG TTGTAATGAT ATTTACCGGA TCAGTGAAAA GCTTGACAGT 
TTAGGAGTGA AGTACGACTC TTCTGAGCCA TCAAAACTTG AACTACCAAC ACCTGGCACT 
CCTATACCAG CTGAAATTCA CTATACACTT CTTATGGATC CAATGAATGT ATTTTACCCT 
GGTGAATATG TTGGTTACCT GGTTGATGCT GAAGGTGGTG ATATATACGG ATCATATCAA 
CCAACTTACA CCTATGCAAT CATTGTACAA GAAGTGGAAA GAGAAGATGA TGAAAATCCC 
AGTTTTCTAG GGAAGATTTA CCAGATCGAT ATTGGATACA GTGAGTATAA AATAGTGAGC 
TCTCTTGACT TGTACAAATT TTCAAGACCG GAGGAAAGTT CTCAAAGCAG GGATAGCACA 
CCTTCTACCC CAACCAGTCC TACAGAATTT CCAGCACACG GACCTAGGGC CATTCCACCT 
CTCTTCGCTG GTAGAGAGAG CCACAAAACA ATGTCCTCAA AACATCATTC TCCAAAAAAG 
ATAAAGTCAA ACTCATTGCC AGAGATATTA AGAGAAGTTA CATCTGTGAT TGAACAGGCT 
TGGAAGCTTC CAGAATCTGA AAGAAAAAAG ATTATTAGGA GGCTGTATCT TAAATGGCAT 
CCAGATAAAA ATGCAGAGAA TCTTGATATA GCTAATGAAG TTTTCAAACA TTTACAGAAT 
GAGATTAACA GGCTAGAGAA ACAGGCCTTC ATGGATCAAA ATATGGACAG AGCCTCGAGA 
AGACCATTTT CATCCTCTGC TTCTCGATTT CAGTCGGATA AATTTTCATT TCAAAGATTT 
TATACTTCAT GGAATCAAGA AGCAACAAGC CACAAATCTG AAAGGCAGCA GTATAAAGAA 
AAGTGTCCAT CTTCCACGGC GCCATCTTAC TCGCAGCGCT TTTTTGTCCC ACCTACCTTC 
AGGTCCGTTG GCAATCCTGT AGAGGCACGT CGGTGGCTTA GGCAGGCCCG AGCTAACTTC 
TCAGCTGCAA GAAATGACCT TCATAAAAAT GCCAATGAAT GGGTATGCTT TAAGTGCTAC 
CTTTCTACTA AACTCGCCTT GATTGCAGCT GACTATGCTG TGAAGGGCAA ATCGGACAAA 
GACGTAAAAC CAGCAGCCCT TGCACAAAAA ATAGAGGAAT ACAGTCAGCA ACTTGAAGGG 
CTTACAAATG ATGTTCAGAC GTTGGAAGCT TACGGAGTAG ATAGCTTAAA AACTAGGTAT 
CCTGACTTAC TGCCTTTCCC ACAGATTCCC AATGATAGAT TTACTTCAGA AGTTGCTATG 
AGAGTGATGG AATGTACTGC TTGTATCATA ATAAAACTTG AGAACTTTAT ACAACAGAAG 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821327.1
CDS	267..13592
Translation	MAQYQGPAFYAYNDAIFTPEDWHGIQEIARSRKKDDPLKVGRFGIGFNSVYHITDVPSIFSGDQIGMLDPHQTLFGPHESGQCWNLKEDSKEINELTDQFAPFIGVFGSTKETFKNGSFPGTFFRFPLRLQPSQLSSNVYDKQKVLELFESFRADADTVLLFLKSVQDVSLHVREADGTERLIFRVTASENKALKHERPNSIKILGTAINQYCKGVPSNSITCVTYHVNIVVEDESVKDAQKTSWLVCNCVGGRGMCTELDSLADDLKFVPTIGIAMSLSTNEEEKGAVADFSGRAFCFLPLPPGEESKTGLPVHVSGFFGLTDNRRSIKWRELDQWRDPAALWNDLLVVNIVPKAYTTLILEAIKRMETEKNSDFPLSAERIYRLWPDENKIRVPWKPIVVPLFKELLQHTVIYSVSNQWIQVEQVHFSEMDESLEYTQSVLNYLQNSGKQIAKVPANIASAVHLTISAAKAVKKVTPAVVRQVLRKSGHSGTAEEKLHLLEFVLSDGVYSELIGLELLPLQNGNFIPFSSSVSEQDVVYITSEEYPRSLFPGLEGRLLSDDLKPEVLAALKEAAKSRAQQLLWGFSGRPCTQLQLLNPERFARLIKEVMNSTWPGRDMVVQWHPGLEDKNHPSVSWLKMVWKNLYIHFSDDLSVFDDMPLIPKTLLEENQTSVELVRLRNPSPIILEDESETQLPEYLADIIQKVGGVVLKQLDISIQHPLIKKYVHPPLPSAVLQIMEKMSLQKLCSQVASFPSTHKDALRAFLASLTDVSEKERRIIQELLIFKKIEKSSDESVPVFTGLKGSKVLHHTAKIPPGLRFSVPIIDSSDEATIRLANLLKIEQLKSTDCLKFVIQDIRSDFYSYDEITQIMQWVLENLTFLKNENTDVIDWLTSLKFIKISEENIMSASELFDPEVEVLQNLFYAEEEICFPPVVFTSSDILHSLRQIGLKNEANLEESDIMRVANKIESLHANSNTDHDLLLRKARTLVMVLNKNHTLLHSSETKAALKKIKWIPACKERPPNYPGSLVWKGDHCNLCSPPEMCDIAHAILVGSSVPLVENVLLDTEKALGIPTKPSIKAVLKHFKVVVDWHSSKTFSDEDYYQFQHILLEIYGFMHDHLEEGKEAFKALKFPWVWTGKTFCSLTQAVIKSVPDLDLQPYLHYVPKTMAKFHQLFICCGSIEQLTPDHVSMVIQKIYLKSEQPLSEQESKQNLHIMLSIIRWLYSNQIPASLHTPMPLYSGKLPYKLAMRPIHECCYCDIKVDDLNDLLEDSVEPIILVHEDIPMKTAEWLNVPCLSTRLINPENMGFEQSGQREPLTVRIKNILEEYPSISDIFKELLQNADDANATECNFLIDMRRNMDIRENLLDPGMAACHGPALWSFNNSEFSDSDFLNITRLGESLKRSEVDKVGKFGLGFNSVYHITDVPIILSREFMIMFDPNVNHISKHIRDKSNPGIKINWSKQQKRLRKFPNQFKPFIGVFGCQLPLTVESPYSYKGTLFRLSFRTQQEAKVSEVSSASYNTADIYSLVDEFSICGHRLIIFAQSVNSMVLKYLKIEADDPGVAQDVVTIKKNLCSSKALTAPNVSVLKEAAKLMKVCNGSSKKLPTETPKSSCILQIVVEEFHHVFRRIADLQSPLFRGTEDDPASFFEMAKSGQMKRSADELPQKTVDSTTWLICSCMDTGDALKFSLHESGRRLGLVPCGGVGVLLSETQDQKWIVKPNCNNIGEVFCYLPLRIKTGLPLHINGCFAVTSNRKEIWKTDTKGRWNTVFMRHVIVKAYIEALCVLRDMAINGELVDYSYSAVWPDPDSVHDDFSVICQGFYEDIAHMKNKEGIKVFSDGFSWVSMKNVRFLDDSILKRPDIGPSAFKIFQKYLKKTGSKNLCAVDLPSWVKTGFEEAGCKYILLENTFSEKQFFSEVFFPNIQEIDAELRDPLMRYVLNEKVEEFSGILRVTPCIPCSLDGHPLVTPSRLIHPEGRVAKLYDAEDGRFPYGTTQDYLNPVILVKLVQLGMAKDDILWEDLMERAESVAEVNKIDHAAACLRSSIIVSLIDEKLKSRDPRAKEFAAKCQTIPFLPFLSKPAGFSLHWKGSDFQPEAMFSATDLFTADHQDTVCLIQPILNENSHSFKGCGALSLAVKEFLGLLKKPAVNLVINQLEEVAKSFDGITLYQENITNACYKYLHEAMLQNESTKAMIIEQLTNCSFILVENVYVDPTKVSFHLNFEAAPYLYQLPNKYKNSFRELFESVGVRQAFTVEDFAVVLELIDQERGNKQLTEDNFQLCRRIISEGIWGLIREKKQEFCEKKYGEILLPDTRLALLPAKSLCYNDCPWIKVKDTTVKYCHGDIPREVAVKLGAIPKRHKALERYASNICFTTLGTEFGQKEKLTSRIKSILNAYPSEKEMLKELLQNADDAKATEICFVFDPRQHPADRIFDEKWAPLQGPALCVYNNQPFTEDDIRGIQNLGKGTKVGNPCKTGQYGIGFNSVYHITDCPSFISGNDILCIFDPHARYAPGSTSTSPGRMFRDLDADFRTQFSDVLDLYLGDHFKLDNCTMFRFPLRNGEMAKVSEISSVPCSDRMVQNLLDKLRTDGAELLMFLNHMEKISICEIEKTTGALNVLYSVQGKITDGDRLKRKQFHASVIDSVSKKKQLSEIPVQQITYTMDTEDSEGNLTTWLICNRSGFSAMEKVSKSVVSAHKNEDITLFPRGGVAACITHNYKKPHRAFCFLPLSLETGLPFHVNGHFALDSARRNLWRDDNGVGVRSDWNSSLMTALIAPAYVELLIQLKKRYFPGTDPTVSVLQNTPVHVVKDTLKKFLSFFPVNRLDIQPDWYCLVKAVYSCIYEDLKRLLPVMRAPNIDGSDLHSAVIITWVNMSTVNKGRPFFDNLLQDELQHLKNTEYNITTRKTVAENVYRLKHLLLEIGFNLVYNCDETANLYHCLVDADIPVTYVTPADVRSFLMTFSFPDSNCHIGKLPCRLQQTNLKLFHSLKLLVDYCFKDAEENEIQIEGLPLLITLDNVLQIFDSKRPKFLTTYHELIPSRKDLFMNTLYLRYNNILLSSEVAKVFDIKSFAELLSSVLPREYKTRNCMKWKENFASESWLKNAWHFISESINIKEEQEDTQAKFDDVVETLKDWTLLPGVRFTVSANHLVVPECDVLLPLSIMHIAVFPNAQSDKVFHALMKTGCIQLALNKICSKDSALVPLLSGCTANIDNPSSILKAIQYMVQTSTFRTEKLAESDFEALLMYFNCNLCHLTSQDDIKILKSLPCYKSISGRYISISKYGTCYVLTKSIPSVEVDRWTQSTSSAFLEEKVHLTDLYTLLGCVSVDDLEVYLKHLLPKIESLSYDAKLEHLIYLKNRLTSIEESSEMKEQLFEKLESILIIHDATNRLKPAKYFYDRTVKVFEVMLPEKFFIPQDFFKKLEQLTKPKNQAAFLPSWVTFLRHIGLKFIISQQQLLQFAKEISMRANTENWSKETLQNTVDVLLHHIFQERTDLLSGNFLKELSLIPFLCPERAPAEFVRFHPQYQEVNGTLPLIRFNGAQVNPKFKQTDVLQLLWTSCPILPEKATPLSIKEQEGSSLGAQEQLEQVLTMLNVNLDPPLDKVINNCRNICNITTLDEDMVKTRAKVLRSIYEFLSTEKREFRFQLRGVSFVMVEEGWKLLKPEEVVINLEYESDFKPYLYKLPLELGTFHQLFKHLGTEDIISTKQYVEVLGRIYKSSEGKQLDPNEMRTVKRVVSGLFKSLQNDSVKVRNDLENMRDFALYLPSQDGRLVKSSILVFDDAPHYKSRIQGNIGVQMLVDLSQCYLGKDHGFHTKLIMLFPQKLRPRLLSSILEEQLDEESPKICQFGALCSLQGRLQLLLSSEQFITGLIRIMKHENDNAFLANEEKAVRLCKALREGLKVSCFEKLQTTLRVKGFAPIPHSKSETFAFLKRYGNAVILLYIQHSDSKDINFLLALAMTLKSATDNLISDTSYLIAMLGCNDIYRISEKLDSLGVKYDSSEPSKLELPTPGTPIPAEIHYTLLMDPMNVFYPGEYVGYLVDAEGGDIYGSYQPTYTYAIIVQEVEREDDENPSFLGKIYQIDIGYSEYKIVSSLDLYKFSRPEESSQSRDSTPSTPTSPTEFPAHGPRAIPPLFAGRESHKTMSSKHHSPKKIKSNSLPEILREVTSVIEQAWKLPESERKKIIRRLYLKWHPDKNAENLDIANEVFKHLQNEINRLEKQAFMDQNMDRASRRPFSSSASRFQSDKFSFQRFYTSWNQEATSHKSERQQYKEKCPSSTAPSYSQRFFVPPTFRSVGNPVEARRWLRQARANFSAARNDLHKNANEWVCFKCYLSTKLALIAADYAVKGKSDKDVKPAALAQKIEEYSQQLEGLTNDVQTLEAYGVDSLKTRYPDLLPFPQIPNDRFTSEVAMRVMECTACIIIKLENFIQQKV

Target ORF information:

RefSeq Version	XM_014965841.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sacsin molecular chaperone (SACS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965841.1

ORF Insert Sequence:

ATGGCGCAAT ATCAGGGGCC AGCATTTTAT GCATACAACG ATGCAATTTT CACCCCTGAG 
GACTGGCATG GTATACAGGA AATAGCAAGA AGCAGGAAGA AAGATGATCC CCTGAAAGTT 
GGAAGATTTG GAATTGGCTT TAATTCAGTT TATCATATAA CAGATGTCCC TAGCATTTTC 
AGTGGTGACC AGATTGGAAT GCTTGATCCC CATCAGACCC TTTTTGGACC TCATGAATCA 
GGCCAGTGCT GGAATCTAAA AGAAGATAGC AAGGAAATCA ATGAACTTAC AGACCAGTTT 
GCTCCGTTCA TTGGTGTGTT TGGCAGCACT AAGGAAACGT TTAAGAATGG AAGCTTTCCT 
GGTACCTTTT TTCGTTTCCC ACTTCGTCTG CAGCCTTCCC AGCTGAGCAG CAATGTTTAC 
GACAAACAGA AGGTTCTGGA GCTGTTTGAA TCCTTCAGAG CAGATGCAGA CACAGTATTG 
CTCTTTCTGA AGAGTGTTCA GGATGTGTCA TTGCATGTTA GGGAAGCTGA TGGAACTGAG 
AGGCTGATTT TTAGAGTAAC TGCTAGTGAG AACAAGGCCT TGAAACATGA AAGACCAAAT 
TCTATCAAGA TCCTAGGAAC TGCAATAAAT CAGTATTGTA AAGGAGTTCC AAGCAATAGC 
ATAACATGTG TGACATACCA TGTAAATATA GTTGTAGAAG ATGAGAGCGT TAAGGACGCA 
CAGAAAACAT CTTGGTTGGT GTGTAATTGT GTGGGTGGTC GAGGAATGTG TACTGAACTG 
GATAGTTTAG CTGATGACTT GAAATTTGTT CCTACTATTG GAATAGCCAT GTCTTTATCA 
ACTAATGAGG AGGAAAAAGG AGCTGTAGCA GACTTTTCAG GAAGAGCGTT TTGTTTTCTG 
CCTTTGCCTC CAGGTGAAGA GAGCAAAACT GGACTCCCAG TTCATGTCAG TGGTTTCTTT 
GGTCTCACGG ACAATAGGAG GAGTATCAAA TGGCGAGAGC TGGATCAATG GAGAGATCCA 
GCAGCTTTGT GGAATGATCT TCTCGTCGTG AATATTGTGC CCAAGGCATA CACCACCCTT 
ATTTTGGAAG CTATCAAACG AATGGAGACT GAGAAGAATT CAGATTTTCC ATTGTCAGCT 
GAACGAATTT ATAGGTTATG GCCTGATGAG AACAAAATCA GGGTACCTTG GAAACCAATT 
GTAGTACCTC TCTTTAAAGA GCTGCTCCAG CATACAGTTA TTTACTCAGT GAGTAATCAG 
TGGATACAGG TTGAACAGGT GCACTTTTCT GAAATGGATG AGAGTTTGGA GTACACACAG 
TCTGTTCTCA ACTACCTCCA GAATTCAGGG AAGCAGATTG CCAAGGTACC AGCAAATATT 
GCTAGTGCAG TGCATCTTAC TATTTCTGCT GCTAAAGCTG TGAAAAAAGT GACTCCTGCT 
GTAGTACGGC AAGTACTAAG GAAATCTGGA CACAGTGGAA CTGCTGAAGA GAAGCTTCAT 
CTTCTAGAAT TTGTGCTTTC TGATGGAGTC TACAGTGAAC TGATTGGACT GGAGCTTTTG 
CCATTACAAA ATGGAAATTT TATTCCTTTT TCCTCATCTG TGTCAGAACA GGATGTAGTT 
TACATAACCT CAGAAGAATA TCCCAGATCA CTCTTTCCTG GCCTGGAAGG AAGGTTACTT 
TCTGATGATC TGAAACCTGA GGTTCTAGCT GCCTTGAAAG AAGCTGCCAA AAGCCGAGCC 
CAACAGCTTC TCTGGGGATT TTCAGGAAGG CCATGCACTC AGCTGCAGCT TCTGAACCCA 
GAAAGATTCG CTCGGCTCAT TAAAGAAGTT ATGAATAGCA CGTGGCCTGG CAGAGACATG 
GTGGTACAGT GGCATCCAGG TTTGGAAGAC AAAAATCACC CATCAGTTTC CTGGCTTAAG 
ATGGTCTGGA AGAACCTATA TATACATTTT TCTGATGACT TGAGTGTGTT TGATGACATG 
CCGCTTATTC CCAAGACACT GCTGGAGGAA AACCAAACAT CAGTGGAACT GGTTAGACTT 
AGGAATCCAT CACCTATCAT TCTGGAAGAT GAATCTGAGA CTCAGCTCCC GGAATATTTA 
GCTGATATTA TTCAGAAAGT TGGTGGAGTT GTACTTAAGC AGCTAGATAT TTCTATCCAA 
CATCCGCTTA TAAAAAAATA CGTGCATCCA CCATTACCAA GTGCTGTCTT ACAAATCATG 
GAAAAAATGT CCTTACAGAA ACTATGCAGT CAGGTTGCAT CATTCCCGTC AACACATAAA 
GATGCTCTTA GGGCATTCTT GGCTAGTTTA ACTGATGTAA GTGAAAAAGA GAGAAGAATT 
ATTCAGGAAT TGCTAATATT TAAAAAAATT GAAAAATCAT CTGATGAAAG TGTCCCTGTT 
TTTACTGGGC TAAAAGGTAG TAAAGTATTA CACCACACTG CCAAAATTCC TCCTGGTCTA 
AGATTTTCTG TTCCAATAAT TGACAGCAGT GATGAAGCTA CTATTCGCTT AGCTAACCTG 
CTAAAAATAG AACAGCTAAA GAGCACAGAT TGCTTGAAGT TTGTTATACA GGACATAAGA 
AGTGATTTTT ATTCATATGA TGAAATAACA CAGATAATGC AATGGGTTCT TGAAAATCTG 
ACTTTTCTGA AAAATGAGAA TACAGATGTG ATAGATTGGC TGACATCACT AAAGTTTATT 
AAGATTTCAG AGGAAAATAT TATGTCAGCA AGCGAACTCT TTGATCCTGA GGTAGAAGTT 
CTGCAAAACT TATTTTATGC TGAGGAGGAA ATTTGCTTCC CTCCTGTTGT CTTTACATCT 
TCAGATATTC TTCATTCTCT GAGACAAATA GGCTTAAAAA ATGAGGCCAA TCTTGAGGAG 
AGTGATATTA TGAGAGTGGC AAATAAAATT GAAAGTTTGC ATGCTAACTC TAACACTGAT 
CATGATCTAC TGTTAAGAAA AGCTAGAACT CTCGTGATGG TTTTGAACAA AAACCATACA 
CTGCTTCACT CATCTGAAAC AAAAGCTGCA CTGAAAAAAA TAAAATGGAT CCCGGCATGT 
AAGGAAAGAC CTCCCAATTA TCCAGGGTCC TTAGTTTGGA AAGGAGACCA TTGCAATCTG 
TGTTCACCAC CAGAAATGTG TGATATAGCT CATGCAATTT TAGTTGGTTC CTCAGTTCCT 
CTTGTGGAAA ATGTGCTGTT GGATACAGAA AAAGCCCTCG GCATCCCCAC CAAGCCCAGC 
ATAAAAGCAG TCTTAAAACA TTTCAAGGTG GTGGTTGATT GGCACAGTTC TAAAACCTTC 
AGTGATGAGG ACTACTATCA GTTTCAGCAT ATCTTGCTTG AGATTTATGG ATTTATGCAT 
GATCATCTAG AAGAAGGCAA AGAAGCTTTT AAAGCCCTGA AATTTCCCTG GGTTTGGACA 
GGTAAAACAT TTTGTTCCCT CACACAAGCA GTAATAAAGT CAGTACCTGA TCTCGATCTC 
CAACCTTATC TACATTATGT GCCAAAAACG ATGGCGAAGT TTCACCAGTT GTTTATATGT 
TGTGGTTCAA TAGAGCAGTT AACACCAGAC CACGTGTCTA TGGTCATTCA GAAAATATAT 
CTGAAAAGCG AGCAGCCTCT CTCGGAACAA GAGAGTAAAC AAAATCTTCA CATTATGTTG 
AGCATCATAA GGTGGCTGTA CAGCAATCAG ATTCCTGCAA GTCTGCACAC GCCCATGCCG 
CTCTACAGTG GCAAACTTCC CTACAAACTT GCCATGAGGC CCATTCACGA GTGCTGTTAC 
TGCGACATCA AAGTGGATGA CTTAAACGAT TTGCTGGAAG ATTCTGTTGA ACCGATAATT 
TTAGTGCATG AAGACATACC GATGAAAACC GCTGAGTGGT TAAATGTCCC ATGCCTTAGC 
ACAAGACTGA TTAATCCAGA AAATATGGGA TTTGAGCAGT CTGGGCAAAG AGAGCCTCTG 
ACTGTAAGAA TTAAAAACAT TTTGGAGGAA TACCCTTCCA TTTCAGATAT CTTTAAAGAG 
CTTCTTCAAA ATGCTGATGA TGCTAATGCA ACGGAATGCA ATTTCCTGAT TGACATGAGA 
AGAAATATGG ATATAAGAGA GAATCTTCTG GATCCAGGAA TGGCAGCATG TCATGGTCCA 
GCTTTGTGGT CATTCAATAA TTCTGAATTT TCTGACTCTG ATTTCCTAAA TATAACTCGA 
TTAGGAGAGT CTTTAAAAAG GAGTGAAGTT GACAAAGTAG GAAAATTTGG ACTGGGATTC 
AATTCTGTCT ATCATATCAC TGATGTTCCT ATTATTTTGA GCCGGGAGTT CATGATTATG 
TTCGATCCAA ACGTTAACCA TATCAGTAAG CATATTAGAG ATAAATCTAA CCCTGGCATC 
AAAATCAACT GGAGTAAACA ACAGAAGAGG CTGCGAAAGT TTCCCAACCA GTTTAAGCCT 
TTTATAGGAG TTTTTGGTTG CCAGTTGCCA CTGACTGTAG AATCTCCTTA CAGCTACAAA 
GGAACTCTTT TCAGGCTGTC CTTTAGAACT CAGCAGGAAG CTAAAGTGAG TGAAGTCAGC 
AGTGCGTCTT ACAATACAGC AGACATTTAC TCTCTTGTGG ATGAGTTTAG CATCTGTGGT 
CATAGACTGA TAATATTTGC TCAGAGTGTA AATTCAATGG TTTTGAAATA CTTGAAAATT 
GAAGCAGATG ATCCTGGGGT GGCACAGGAT GTTGTCACTA TCAAAAAAAA TCTGTGTTCT 
TCCAAAGCCT TGACTGCCCC AAACGTAAGT GTTTTAAAGG AAGCGGCTAA ACTCATGAAG 
GTCTGCAATG GCAGTAGTAA AAAACTTCCC ACTGAAACAC CAAAGTCCTC GTGTATCTTA 
CAGATAGTAG TAGAAGAATT TCACCATGTA TTTAGACGGA TTGCTGATTT ACAGTCTCCA 
CTTTTCAGAG GTACAGAAGA TGATCCAGCT TCTTTCTTTG AAATGGCTAA ATCAGGACAG 
ATGAAGAGAT CAGCTGATGA ATTGCCACAG AAAACAGTAG ATTCAACAAC ATGGCTTATA 
TGTTCTTGCA TGGACACTGG AGATGCTTTA AAATTTTCAT TACATGAAAG TGGACGAAGA 
CTAGGTCTTG TTCCCTGTGG TGGAGTAGGA GTGTTGCTCT CTGAAACTCA GGATCAAAAA 
TGGATTGTGA AACCTAATTG CAACAACATA GGTGAAGTAT TCTGCTATTT ACCTCTACGA 
ATCAAAACAG GCTTACCTCT TCATATAAAT GGCTGCTTTG CAGTTACTTC AAATCGGAAA 
GAAATCTGGA AAACGGACAC AAAGGGAAGA TGGAATACAG TATTCATGAG GCATGTTATT 
GTAAAAGCCT ACATAGAAGC CCTTTGTGTT CTGCGTGACA TGGCAATCAA TGGTGAGCTT 
GTTGACTACA GTTACTCTGC AGTGTGGCCA GATCCTGATT CAGTTCATGA TGATTTTTCT 
GTTATTTGTC AAGGGTTTTA TGAAGACATA GCTCACATGA AGAACAAAGA AGGGATCAAA 
GTGTTTTCTG ATGGTTTCTC TTGGGTTTCC ATGAAGAATG TAAGGTTTTT AGATGACTCT 
ATACTGAAAC GACCAGATAT TGGACCATCA GCCTTTAAGA TCTTTCAGAA GTACCTTAAA 
AAAACTGGTT CTAAAAATCT GTGTGCTGTA GATCTTCCTT CCTGGGTAAA GACAGGATTT 
GAAGAAGCAG GATGCAAGTA TATATTACTG GAGAACACTT TTTCTGAGAA ACAGTTCTTT 
TCTGAGGTGT TTTTCCCTAA CATTCAGGAG ATTGATGCAG AGCTGCGTGA TCCTTTGATG 
CGTTATGTTC TCAATGAAAA AGTTGAGGAG TTCTCAGGAA TTCTTCGTGT TACTCCGTGT 
ATTCCCTGTT CGTTGGATGG ACATCCATTA GTTACACCAT CAAGACTGAT CCATCCTGAA 
GGAAGAGTTG CAAAATTATA TGATGCTGAG GATGGAAGAT TTCCTTACGG CACCACTCAG 
GATTATCTTA ACCCAGTTAT TTTGGTTAAA CTTGTTCAGT TGGGAATGGC TAAAGATGAT 
ATTTTATGGG AAGATCTGAT GGAACGTGCA GAGTCAGTAG CCGAAGTTAA CAAGATTGAT 
CATGCTGCAG CTTGTCTTAG AAGCAGCATT ATAGTGAGTC TTATTGATGA AAAACTAAAA 
TCTAGGGACC CTAGAGCTAA AGAATTTGCT GCAAAATGTC AAACCATCCC TTTCCTTCCT 
TTCCTTAGCA AACCAGCAGG CTTCTCACTG CACTGGAAAG GCAGTGATTT TCAGCCTGAA 
GCAATGTTTT CAGCAACTGA TCTTTTCACT GCTGATCATC AAGATACAGT TTGCCTAATA 
CAACCCATTC TTAATGAAAA TTCCCACTCC TTTAAAGGTT GTGGTGCTTT GTCATTAGCT 
GTCAAAGAAT TTTTGGGTTT ACTGAAGAAA CCAGCTGTTA ATTTGGTCAT AAATCAGTTA 
GAAGAAGTTG CAAAGTCATT TGATGGAATC ACGTTATATC AAGAAAATAT CACTAATGCT 
TGTTACAAAT ATCTACATGA AGCAATGTTA CAGAACGAAT CAACGAAAGC TATGATAATT 
GAACAACTGA CAAACTGTAG TTTTATTCTG GTTGAGAATG TGTATGTTGA TCCAACAAAA 
GTGTCTTTTC ATTTGAATTT TGAAGCAGCA CCCTACCTCT ATCAGTTGCC TAACAAATAT 
AAAAATAGTT TCCGTGAGCT GTTTGAAAGC GTGGGGGTAA GACAGGCCTT TACAGTTGAA 
GATTTTGCTG TAGTTCTGGA ATTAATAGAT CAGGAAAGAG GGAACAAACA ACTAACAGAA 
GACAACTTTC AGCTTTGCAG GAGAATAATT AGTGAAGGAA TATGGGGCCT AATTAGAGAG 
AAGAAGCAGG AATTTTGTGA GAAAAAGTAC GGTGAGATTT TGTTACCTGA CACTCGTCTT 
GCACTTCTGC CTGCGAAATC TTTGTGTTAC AATGACTGTC CATGGATTAA GGTTAAAGAC 
ACAACCGTTA AATATTGTCA TGGTGATATT CCAAGAGAAG TTGCAGTAAA GCTTGGAGCA 
ATCCCAAAAC GTCATAAAGC TTTAGAAAGA TATGCATCCA ATATCTGTTT TACTACCCTT 
GGAACAGAGT TTGGCCAGAA AGAAAAATTG ACAAGTAGAA TTAAAAGCAT TCTTAATGCC 
TACCCGTCAG AAAAAGAAAT GCTGAAAGAA CTCCTTCAGA ATGCAGATGA TGCAAAAGCT 
ACAGAGATCT GTTTTGTGTT TGATCCTAGG CAGCATCCAG CTGATAGAAT ATTTGATGAG 
AAATGGGCAC CACTTCAAGG ACCAGCACTG TGTGTTTACA ACAACCAGCC TTTTACAGAA 
GATGATATTC GAGGAATTCA GAACCTTGGA AAAGGTACAA AAGTAGGAAA TCCCTGTAAA 
ACTGGACAAT ATGGTATAGG TTTCAATTCT GTTTATCACA TTACTGATTG CCCTTCTTTC 
ATATCTGGCA ATGATATACT TTGTATTTTT GATCCTCATG CTAGATATGC ACCAGGTTCA 
ACATCAACAA GTCCTGGCCG CATGTTTAGA GATTTAGATG CAGATTTCAG AACACAGTTC 
TCAGATGTGC TGGACCTTTA CTTAGGAGAT CACTTTAAAC TGGATAATTG CACAATGTTT 
AGGTTTCCTC TTCGAAATGG AGAAATGGCA AAAGTATCAG AAATTTCCTC AGTTCCATGT 
TCGGATAGAA TGGTCCAAAA CCTATTAGAT AAGCTGCGTA CAGATGGAGC AGAACTCTTA 
ATGTTTTTGA ACCATATGGA AAAAATTTCT ATTTGTGAGA TAGAAAAAAC AACAGGAGCA 
CTGAATGTGT TGTATTCTGT GCAAGGCAAA ATCACTGATG GAGACAGACT GAAACGAAAG 
CAATTCCATG CATCTGTAAT TGACAGCGTA AGTAAAAAAA AGCAGCTAAG TGAAATACCC 
GTGCAACAGA TTACTTACAC AATGGATACT GAAGACTCTG AAGGAAATCT TACAACTTGG 
TTAATTTGTA ACAGGTCAGG CTTTTCTGCC ATGGAAAAGG TATCCAAAAG TGTGGTTTCA 
GCCCACAAGA ATGAGGACAT TACTCTTTTT CCACGTGGCG GGGTAGCAGC TTGCATTACG 
CATAATTACA AAAAGCCCCA CAGAGCGTTC TGTTTCTTAC CGTTATCGTT AGAAACTGGA 
TTACCCTTTC ACGTGAATGG ACACTTTGCC CTGGATTCTG CAAGAAGAAA TCTGTGGCGT 
GATGATAACG GTGTTGGAGT AAGAAGTGAC TGGAATAGTA GCCTAATGAC AGCGCTGATT 
GCACCGGCCT ACGTTGAACT GCTGATTCAG CTGAAGAAAC GGTATTTCCC AGGCACTGAT 
CCTACAGTGT CCGTGCTGCA AAACACACCT GTTCATGTTG TGAAAGACAC TTTAAAAAAA 
TTTTTATCCT TTTTTCCAGT TAATAGACTT GATATTCAGC CGGATTGGTA TTGCTTAGTG 
AAAGCAGTTT ACAGTTGCAT TTATGAAGAT CTGAAGCGTC TTTTACCTGT TATGCGAGCT 
CCAAATATCG ATGGTTCTGA TTTGCATTCT GCTGTTATCA TCACATGGGT TAATATGTCT 
ACAGTGAACA AAGGCAGGCC ATTCTTTGAC AATTTACTAC AAGATGAATT GCAACATCTC 
AAAAATACAG AATACAACAT CACAACGCGG AAGACAGTGG CAGAAAATGT CTACAGACTC 
AAGCATTTAC TCTTAGAAAT TGGATTTAAT TTGGTATATA ACTGTGATGA AACTGCAAAT 
CTTTACCACT GTCTGGTAGA TGCAGATATT CCTGTCACCT ATGTGACACC TGCTGATGTC 
CGTTCATTTT TGATGACATT TTCTTTTCCA GACTCTAATT GCCACATTGG AAAGTTACCC 
TGTCGTCTTC AGCAGACGAA TTTGAAACTT TTCCATAGTC TCAAACTTCT GGTTGACTAC 
TGTTTTAAAG ATGCAGAAGA AAATGAAATC CAGATTGAGG GCTTGCCACT CCTTATTACT 
CTTGACAATG TTTTGCAAAT TTTTGATTCA AAGCGACCAA AGTTCTTAAC AACATACCAT 
GAGCTGATTC CATCTCGCAA GGATCTCTTT ATGAACACGT TGTACTTGAG ATACAACAAT 
ATTTTACTGA GCAGTGAAGT AGCGAAAGTC TTTGATATCA AAAGTTTTGC TGAATTGTTA 
TCATCTGTGT TGCCGAGAGA ATATAAAACC AGAAATTGTA TGAAATGGAA GGAAAACTTT 
GCAAGTGAGT CTTGGCTTAA AAACGCCTGG CACTTTATTA GTGAGTCTAT AAATATAAAG 
GAAGAGCAAG AAGATACGCA AGCAAAATTC GATGATGTTG TTGAAACTTT GAAGGACTGG 
ACTTTGCTCC CAGGTGTAAG GTTTACTGTC TCAGCTAATC ACCTTGTTGT GCCAGAGTGT 
GACGTTCTGC TGCCACTCAG CATTATGCAT ATAGCTGTTT TTCCAAATGC TCAGAGTGAT 
AAAGTCTTCC ATGCTTTAAT GAAAACAGGC TGTATTCAGT TGGCATTGAA CAAAATTTGC 
TCCAAAGACA GTGCTTTAGT GCCTTTGTTG TCAGGATGTA CAGCAAATAT AGATAATCCT 
TCAAGCATTC TGAAAGCCAT ACAATATATG GTACAGACAT CAACTTTCAG GACTGAAAAG 
TTAGCAGAAA GTGACTTTGA GGCTCTCTTA ATGTACTTCA ATTGCAATTT ATGTCATTTG 
ACATCTCAGG ATGACATTAA AATTTTAAAG TCTCTTCCAT GTTATAAATC GATCAGTGGC 
CGATACATCA GCATTTCAAA ATACGGGACG TGTTACGTTC TCACGAAAAG CATCCCATCA 
GTAGAAGTAG ACAGATGGAC ACAGTCAACT TCATCAGCTT TTCTTGAAGA GAAGGTTCAT 
TTGACAGATT TGTACACTCT GCTTGGCTGT GTCTCTGTGG ATGATCTTGA AGTGTACTTG 
AAACATCTTT TGCCAAAAAT TGAGAGCCTG TCTTACGATG CAAAATTAGA ACACTTGATC 
TACCTAAAGA ATAGGCTAAC TAGCATTGAG GAATCTTCAG AAATGAAAGA ACAGCTGTTT 
GAAAAGCTTG AAAGCATTTT GATCATTCAT GATGCAACCA ATAGATTAAA GCCTGCAAAA 
TATTTTTATG ACAGGACTGT AAAAGTCTTT GAAGTTATGC TTCCTGAAAA GTTCTTTATA 
CCTCAGGATT TTTTTAAGAA ACTCGAGCAA CTTACAAAAC CAAAAAACCA AGCTGCATTC 
CTTCCATCAT GGGTAACTTT TCTACGACAT ATCGGATTGA AATTCATCAT TTCGCAGCAA 
CAGTTGCTAC AGTTTGCTAA GGAGATCAGC ATGCGAGCGA ATACAGAAAA CTGGTCTAAA 
GAAACACTAC AGAATACTGT CGATGTCCTC CTTCATCACA TATTTCAAGA AAGAACTGAC 
CTTTTGTCAG GAAATTTTCT GAAAGAATTG TCATTAATTC CTTTTCTGTG CCCTGAACGA 
GCTCCAGCAG AATTTGTTAG ATTTCATCCT CAGTATCAAG AAGTTAATGG GACACTTCCT 
CTTATACGAT TCAATGGGGC ACAGGTGAAT CCTAAATTCA AACAGACAGA TGTGTTGCAG 
TTGCTGTGGA CTTCATGTCC CATTCTTCCT GAGAAAGCAA CACCTTTAAG CATCAAGGAG 
CAAGAGGGAA GTAGTCTTGG TGCACAAGAA CAACTTGAGC AAGTTTTAAC TATGCTGAAT 
GTCAATTTGG ACCCTCCCCT TGACAAAGTT ATCAATAACT GCAGGAACAT ATGCAATATA 
ACGACTTTGG ATGAGGACAT GGTGAAAACT AGGGCAAAGG TCTTAAGAAG CATTTATGAA 
TTTCTTAGTA CAGAGAAAAG GGAATTCCGC TTTCAACTTC GAGGAGTTTC TTTTGTAATG 
GTGGAAGAAG GTTGGAAGCT TCTGAAGCCT GAAGAGGTGG TAATAAACCT TGAGTATGAA 
TCTGATTTTA AACCTTATCT TTACAAACTT CCTTTAGAAC TTGGTACTTT CCATCAGTTA 
TTTAAACATT TGGGTACTGA AGATATTATT TCAACAAAGC AGTATGTTGA AGTTCTAGGT 
CGCATATACA AGAGCTCGGA AGGAAAGCAA TTGGATCCTA ATGAAATGCG CACGGTTAAA 
AGAGTAGTTT CTGGCCTGTT CAAAAGTCTT CAGAATGATT CTGTCAAGGT TAGGAATGAC 
CTTGAGAATA TGAGGGATTT TGCCCTTTAC CTTCCGAGTC AAGATGGTAG GTTGGTAAAG 
TCTAGTATCC TAGTTTTTGA TGATGCACCT CACTACAAAA GCAGAATCCA AGGTAACATT 
GGTGTGCAAA TGCTTGTTGA TCTTAGCCAG TGTTACTTAG GTAAAGACCA TGGCTTTCAC 
ACTAAATTGA TTATGTTATT CCCTCAGAAA TTGAGGCCTC GCTTACTTAG CAGTATTCTC 
GAAGAACAGT TGGATGAAGA GTCTCCCAAA ATCTGTCAGT TTGGAGCATT ATGTTCTTTG 
CAGGGAAGGT TACAGTTACT GTTGTCTTCG GAACAATTCA TCACAGGACT CATTAGGATT 
ATGAAGCATG AAAATGACAA TGCTTTTTTA GCTAATGAGG AAAAAGCAGT AAGACTTTGC 
AAAGCTTTGC GAGAAGGCTT GAAAGTTTCC TGTTTTGAGA AGTTGCAAAC AACGTTACGA 
GTTAAAGGCT TTGCTCCTAT TCCCCACAGC AAAAGCGAAA CATTTGCTTT CCTGAAGAGA 
TACGGAAATG CAGTAATACT GCTTTACATA CAGCATTCTG ACAGCAAAGA CATAAATTTC 
CTGTTGGCAT TAGCCATGAC CTTAAAATCT GCAACTGACA ACCTGATTTC TGATACCTCA 
TATTTAATTG CCATGCTGGG TTGTAATGAT ATTTACCGGA TCAGTGAAAA GCTTGACAGT 
TTAGGAGTGA AGTACGACTC TTCTGAGCCA TCAAAACTTG AACTACCAAC ACCTGGCACT 
CCTATACCAG CTGAAATTCA CTATACACTT CTTATGGATC CAATGAATGT ATTTTACCCT 
GGTGAATATG TTGGTTACCT GGTTGATGCT GAAGGTGGTG ATATATACGG ATCATATCAA 
CCAACTTACA CCTATGCAAT CATTGTACAA GAAGTGGAAA GAGAAGATGA TGAAAATCCC 
AGTTTTCTAG GGAAGATTTA CCAGATCGAT ATTGGATACA GTGAGTATAA AATAGTGAGC 
TCTCTTGACT TGTACAAATT TTCAAGACCG GAGGAAAGTT CTCAAAGCAG GGATAGCACA 
CCTTCTACCC CAACCAGTCC TACAGAATTT CCAGCACACG GACCTAGGGC CATTCCACCT 
CTCTTCGCTG GTAGAGAGAG CCACAAAACA ATGTCCTCAA AACATCATTC TCCAAAAAAG 
ATAAAGTCAA ACTCATTGCC AGAGATATTA AGAGAAGTTA CATCTGTGAT TGAACAGGCT 
TGGAAGCTTC CAGAATCTGA AAGAAAAAAG ATTATTAGGA GGCTGTATCT TAAATGGCAT 
CCAGATAAAA ATGCAGAGAA TCTTGATATA GCTAATGAAG TTTTCAAACA TTTACAGAAT 
GAGATTAACA GGCTAGAGAA ACAGGCCTTC ATGGATCAAA ATATGGACAG AGCCTCGAGA 
AGACCATTTT CATCCTCTGC TTCTCGATTT CAGTCGGATA AATTTTCATT TCAAAGATTT 
TATACTTCAT GGAATCAAGA AGCAACAAGC CACAAATCTG AAAGGCAGCA GTATAAAGAA 
AAGTGTCCAT CTTCCACGGC GCCATCTTAC TCGCAGCGCT TTTTTGTCCC ACCTACCTTC 
AGGTCCGTTG GCAATCCTGT AGAGGCACGT CGGTGGCTTA GGCAGGCCCG AGCTAACTTC 
TCAGCTGCAA GAAATGACCT TCATAAAAAT GCCAATGAAT GGGTATGCTT TAAGTGCTAC 
CTTTCTACTA AACTCGCCTT GATTGCAGCT GACTATGCTG TGAAGGGCAA ATCGGACAAA 
GACGTAAAAC CAGCAGCCCT TGCACAAAAA ATAGAGGAAT ACAGTCAGCA ACTTGAAGGG 
CTTACAAATG ATGTTCAGAC GTTGGAAGCT TACGGAGTAG ATAGCTTAAA AACTAGGTAT 
CCTGACTTAC TGCCTTTCCC ACAGATTCCC AATGATAGAT TTACTTCAGA AGTTGCTATG 
AGAGTGATGG AATGTACTGC TTGTATCATA ATAAAACTTG AGAACTTTAT ACAACAGAAG 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SACS ( XM_014965842.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821328.1 Calidris pugnax sacsin molecular chaperone (SACS), transcript variant X2, mRNA.

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CloneID	OCa82207
Clone ID Related Accession (Same CDS sequence)	XM_014965842.1
Accession Version	XM_014965842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094510.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAT ATCAGGGGCC AGCATTTTAT GCATACAACG ATGCAATTTT CACCCCTGAG 
GACTGGCATG GTATACAGGA AATAGCAAGA AGCAGGAAGA AAGATGATCC CCTGAAAGTT 
GGAAGATTTG GAATTGGCTT TAATTCAGTT TATCATATAA CAGATGTCCC TAGCATTTTC 
AGTGGTGACC AGATTGGAAT GCTTGATCCC CATCAGACCC TTTTTGGACC TCATGAATCA 
GGCCAGTGCT GGAATCTAAA AGAAGATAGC AAGGAAATCA ATGAACTTAC AGACCAGTTT 
GCTCCGTTCA TTGGTGTGTT TGGCAGCACT AAGGAAACGT TTAAGAATGG AAGCTTTCCT 
GGTACCTTTT TTCGTTTCCC ACTTCGTCTG CAGCCTTCCC AGCTGAGCAG CAATGTTTAC 
GACAAACAGA AGGTTCTGGA GCTGTTTGAA TCCTTCAGAG CAGATGCAGA CACAGTATTG 
CTCTTTCTGA AGAGTGTTCA GGATGTGTCA TTGCATGTTA GGGAAGCTGA TGGAACTGAG 
AGGCTGATTT TTAGAGTAAC TGCTAGTGAG AACAAGGCCT TGAAACATGA AAGACCAAAT 
TCTATCAAGA TCCTAGGAAC TGCAATAAAT CAGTATTGTA AAGGAGTTCC AAGCAATAGC 
ATAACATGTG TGACATACCA TGTAAATATA GTTGTAGAAG ATGAGAGCGT TAAGGACGCA 
CAGAAAACAT CTTGGTTGGT GTGTAATTGT GTGGGTGGTC GAGGAATGTG TACTGAACTG 
GATAGTTTAG CTGATGACTT GAAATTTGTT CCTACTATTG GAATAGCCAT GTCTTTATCA 
ACTAATGAGG AGGAAAAAGG AGCTGTAGCA GACTTTTCAG GAAGAGCGTT TTGTTTTCTG 
CCTTTGCCTC CAGGTGAAGA GAGCAAAACT GGACTCCCAG TTCATGTCAG TGGTTTCTTT 
GGTCTCACGG ACAATAGGAG GAGTATCAAA TGGCGAGAGC TGGATCAATG GAGAGATCCA 
GCAGCTTTGT GGAATGATCT TCTCGTCGTG AATATTGTGC CCAAGGCATA CACCACCCTT 
ATTTTGGAAG CTATCAAACG AATGGAGACT GAGAAGAATT CAGATTTTCC ATTGTCAGCT 
GAACGAATTT ATAGGTTATG GCCTGATGAG AACAAAATCA GGGTACCTTG GAAACCAATT 
GTAGTACCTC TCTTTAAAGA GCTGCTCCAG CATACAGTTA TTTACTCAGT GAGTAATCAG 
TGGATACAGG TTGAACAGGT GCACTTTTCT GAAATGGATG AGAGTTTGGA GTACACACAG 
TCTGTTCTCA ACTACCTCCA GAATTCAGGG AAGCAGATTG CCAAGGTACC AGCAAATATT 
GCTAGTGCAG TGCATCTTAC TATTTCTGCT GCTAAAGCTG TGAAAAAAGT GACTCCTGCT 
GTAGTACGGC AAGTACTAAG GAAATCTGGA CACAGTGGAA CTGCTGAAGA GAAGCTTCAT 
CTTCTAGAAT TTGTGCTTTC TGATGGAGTC TACAGTGAAC TGATTGGACT GGAGCTTTTG 
CCATTACAAA ATGGAAATTT TATTCCTTTT TCCTCATCTG TGTCAGAACA GGATGTAGTT 
TACATAACCT CAGAAGAATA TCCCAGATCA CTCTTTCCTG GCCTGGAAGG AAGGTTACTT 
TCTGATGATC TGAAACCTGA GGTTCTAGCT GCCTTGAAAG AAGCTGCCAA AAGCCGAGGA 
AGGCCATGCA CTCAGCTGCA GCTTCTGAAC CCAGAAAGAT TCGCTCGGCT CATTAAAGAA 
GTTATGAATA GCACGTGGCC TGGCAGAGAC ATGGTGGTAC AGTGGCATCC AGGTTTGGAA 
GACAAAAATC ACCCATCAGT TTCCTGGCTT AAGATGGTCT GGAAGAACCT ATATATACAT 
TTTTCTGATG ACTTGAGTGT GTTTGATGAC ATGCCGCTTA TTCCCAAGAC ACTGCTGGAG 
GAAAACCAAA CATCAGTGGA ACTGGTTAGA CTTAGGAATC CATCACCTAT CATTCTGGAA 
GATGAATCTG AGACTCAGCT CCCGGAATAT TTAGCTGATA TTATTCAGAA AGTTGGTGGA 
GTTGTACTTA AGCAGCTAGA TATTTCTATC CAACATCCGC TTATAAAAAA ATACGTGCAT 
CCACCATTAC CAAGTGCTGT CTTACAAATC ATGGAAAAAA TGTCCTTACA GAAACTATGC 
AGTCAGGTTG CATCATTCCC GTCAACACAT AAAGATGCTC TTAGGGCATT CTTGGCTAGT 
TTAACTGATG TAAGTGAAAA AGAGAGAAGA ATTATTCAGG AATTGCTAAT ATTTAAAAAA 
ATTGAAAAAT CATCTGATGA AAGTGTCCCT GTTTTTACTG GGCTAAAAGG TAGTAAAGTA 
TTACACCACA CTGCCAAAAT TCCTCCTGGT CTAAGATTTT CTGTTCCAAT AATTGACAGC 
AGTGATGAAG CTACTATTCG CTTAGCTAAC CTGCTAAAAA TAGAACAGCT AAAGAGCACA 
GATTGCTTGA AGTTTGTTAT ACAGGACATA AGAAGTGATT TTTATTCATA TGATGAAATA 
ACACAGATAA TGCAATGGGT TCTTGAAAAT CTGACTTTTC TGAAAAATGA GAATACAGAT 
GTGATAGATT GGCTGACATC ACTAAAGTTT ATTAAGATTT CAGAGGAAAA TATTATGTCA 
GCAAGCGAAC TCTTTGATCC TGAGGTAGAA GTTCTGCAAA ACTTATTTTA TGCTGAGGAG 
GAAATTTGCT TCCCTCCTGT TGTCTTTACA TCTTCAGATA TTCTTCATTC TCTGAGACAA 
ATAGGCTTAA AAAATGAGGC CAATCTTGAG GAGAGTGATA TTATGAGAGT GGCAAATAAA 
ATTGAAAGTT TGCATGCTAA CTCTAACACT GATCATGATC TACTGTTAAG AAAAGCTAGA 
ACTCTCGTGA TGGTTTTGAA CAAAAACCAT ACACTGCTTC ACTCATCTGA AACAAAAGCT 
GCACTGAAAA AAATAAAATG GATCCCGGCA TGTAAGGAAA GACCTCCCAA TTATCCAGGG 
TCCTTAGTTT GGAAAGGAGA CCATTGCAAT CTGTGTTCAC CACCAGAAAT GTGTGATATA 
GCTCATGCAA TTTTAGTTGG TTCCTCAGTT CCTCTTGTGG AAAATGTGCT GTTGGATACA 
GAAAAAGCCC TCGGCATCCC CACCAAGCCC AGCATAAAAG CAGTCTTAAA ACATTTCAAG 
GTGGTGGTTG ATTGGCACAG TTCTAAAACC TTCAGTGATG AGGACTACTA TCAGTTTCAG 
CATATCTTGC TTGAGATTTA TGGATTTATG CATGATCATC TAGAAGAAGG CAAAGAAGCT 
TTTAAAGCCC TGAAATTTCC CTGGGTTTGG ACAGGTAAAA CATTTTGTTC CCTCACACAA 
GCAGTAATAA AGTCAGTACC TGATCTCGAT CTCCAACCTT ATCTACATTA TGTGCCAAAA 
ACGATGGCGA AGTTTCACCA GTTGTTTATA TGTTGTGGTT CAATAGAGCA GTTAACACCA 
GACCACGTGT CTATGGTCAT TCAGAAAATA TATCTGAAAA GCGAGCAGCC TCTCTCGGAA 
CAAGAGAGTA AACAAAATCT TCACATTATG TTGAGCATCA TAAGGTGGCT GTACAGCAAT 
CAGATTCCTG CAAGTCTGCA CACGCCCATG CCGCTCTACA GTGGCAAACT TCCCTACAAA 
CTTGCCATGA GGCCCATTCA CGAGTGCTGT TACTGCGACA TCAAAGTGGA TGACTTAAAC 
GATTTGCTGG AAGATTCTGT TGAACCGATA ATTTTAGTGC ATGAAGACAT ACCGATGAAA 
ACCGCTGAGT GGTTAAATGT CCCATGCCTT AGCACAAGAC TGATTAATCC AGAAAATATG 
GGATTTGAGC AGTCTGGGCA AAGAGAGCCT CTGACTGTAA GAATTAAAAA CATTTTGGAG 
GAATACCCTT CCATTTCAGA TATCTTTAAA GAGCTTCTTC AAAATGCTGA TGATGCTAAT 
GCAACGGAAT GCAATTTCCT GATTGACATG AGAAGAAATA TGGATATAAG AGAGAATCTT 
CTGGATCCAG GAATGGCAGC ATGTCATGGT CCAGCTTTGT GGTCATTCAA TAATTCTGAA 
TTTTCTGACT CTGATTTCCT AAATATAACT CGATTAGGAG AGTCTTTAAA AAGGAGTGAA 
GTTGACAAAG TAGGAAAATT TGGACTGGGA TTCAATTCTG TCTATCATAT CACTGATGTT 
CCTATTATTT TGAGCCGGGA GTTCATGATT ATGTTCGATC CAAACGTTAA CCATATCAGT 
AAGCATATTA GAGATAAATC TAACCCTGGC ATCAAAATCA ACTGGAGTAA ACAACAGAAG 
AGGCTGCGAA AGTTTCCCAA CCAGTTTAAG CCTTTTATAG GAGTTTTTGG TTGCCAGTTG 
CCACTGACTG TAGAATCTCC TTACAGCTAC AAAGGAACTC TTTTCAGGCT GTCCTTTAGA 
ACTCAGCAGG AAGCTAAAGT GAGTGAAGTC AGCAGTGCGT CTTACAATAC AGCAGACATT 
TACTCTCTTG TGGATGAGTT TAGCATCTGT GGTCATAGAC TGATAATATT TGCTCAGAGT 
GTAAATTCAA TGGTTTTGAA ATACTTGAAA ATTGAAGCAG ATGATCCTGG GGTGGCACAG 
GATGTTGTCA CTATCAAAAA AAATCTGTGT TCTTCCAAAG CCTTGACTGC CCCAAACGTA 
AGTGTTTTAA AGGAAGCGGC TAAACTCATG AAGGTCTGCA ATGGCAGTAG TAAAAAACTT 
CCCACTGAAA CACCAAAGTC CTCGTGTATC TTACAGATAG TAGTAGAAGA ATTTCACCAT 
GTATTTAGAC GGATTGCTGA TTTACAGTCT CCACTTTTCA GAGGTACAGA AGATGATCCA 
GCTTCTTTCT TTGAAATGGC TAAATCAGGA CAGATGAAGA GATCAGCTGA TGAATTGCCA 
CAGAAAACAG TAGATTCAAC AACATGGCTT ATATGTTCTT GCATGGACAC TGGAGATGCT 
TTAAAATTTT CATTACATGA AAGTGGACGA AGACTAGGTC TTGTTCCCTG TGGTGGAGTA 
GGAGTGTTGC TCTCTGAAAC TCAGGATCAA AAATGGATTG TGAAACCTAA TTGCAACAAC 
ATAGGTGAAG TATTCTGCTA TTTACCTCTA CGAATCAAAA CAGGCTTACC TCTTCATATA 
AATGGCTGCT TTGCAGTTAC TTCAAATCGG AAAGAAATCT GGAAAACGGA CACAAAGGGA 
AGATGGAATA CAGTATTCAT GAGGCATGTT ATTGTAAAAG CCTACATAGA AGCCCTTTGT 
GTTCTGCGTG ACATGGCAAT CAATGGTGAG CTTGTTGACT ACAGTTACTC TGCAGTGTGG 
CCAGATCCTG ATTCAGTTCA TGATGATTTT TCTGTTATTT GTCAAGGGTT TTATGAAGAC 
ATAGCTCACA TGAAGAACAA AGAAGGGATC AAAGTGTTTT CTGATGGTTT CTCTTGGGTT 
TCCATGAAGA ATGTAAGGTT TTTAGATGAC TCTATACTGA AACGACCAGA TATTGGACCA 
TCAGCCTTTA AGATCTTTCA GAAGTACCTT AAAAAAACTG GTTCTAAAAA TCTGTGTGCT 
GTAGATCTTC CTTCCTGGGT AAAGACAGGA TTTGAAGAAG CAGGATGCAA GTATATATTA 
CTGGAGAACA CTTTTTCTGA GAAACAGTTC TTTTCTGAGG TGTTTTTCCC TAACATTCAG 
GAGATTGATG CAGAGCTGCG TGATCCTTTG ATGCGTTATG TTCTCAATGA AAAAGTTGAG 
GAGTTCTCAG GAATTCTTCG TGTTACTCCG TGTATTCCCT GTTCGTTGGA TGGACATCCA 
TTAGTTACAC CATCAAGACT GATCCATCCT GAAGGAAGAG TTGCAAAATT ATATGATGCT 
GAGGATGGAA GATTTCCTTA CGGCACCACT CAGGATTATC TTAACCCAGT TATTTTGGTT 
AAACTTGTTC AGTTGGGAAT GGCTAAAGAT GATATTTTAT GGGAAGATCT GATGGAACGT 
GCAGAGTCAG TAGCCGAAGT TAACAAGATT GATCATGCTG CAGCTTGTCT TAGAAGCAGC 
ATTATAGTGA GTCTTATTGA TGAAAAACTA AAATCTAGGG ACCCTAGAGC TAAAGAATTT 
GCTGCAAAAT GTCAAACCAT CCCTTTCCTT CCTTTCCTTA GCAAACCAGC AGGCTTCTCA 
CTGCACTGGA AAGGCAGTGA TTTTCAGCCT GAAGCAATGT TTTCAGCAAC TGATCTTTTC 
ACTGCTGATC ATCAAGATAC AGTTTGCCTA ATACAACCCA TTCTTAATGA AAATTCCCAC 
TCCTTTAAAG GTTGTGGTGC TTTGTCATTA GCTGTCAAAG AATTTTTGGG TTTACTGAAG 
AAACCAGCTG TTAATTTGGT CATAAATCAG TTAGAAGAAG TTGCAAAGTC ATTTGATGGA 
ATCACGTTAT ATCAAGAAAA TATCACTAAT GCTTGTTACA AATATCTACA TGAAGCAATG 
TTACAGAACG AATCAACGAA AGCTATGATA ATTGAACAAC TGACAAACTG TAGTTTTATT 
CTGGTTGAGA ATGTGTATGT TGATCCAACA AAAGTGTCTT TTCATTTGAA TTTTGAAGCA 
GCACCCTACC TCTATCAGTT GCCTAACAAA TATAAAAATA GTTTCCGTGA GCTGTTTGAA 
AGCGTGGGGG TAAGACAGGC CTTTACAGTT GAAGATTTTG CTGTAGTTCT GGAATTAATA 
GATCAGGAAA GAGGGAACAA ACAACTAACA GAAGACAACT TTCAGCTTTG CAGGAGAATA 
ATTAGTGAAG GAATATGGGG CCTAATTAGA GAGAAGAAGC AGGAATTTTG TGAGAAAAAG 
TACGGTGAGA TTTTGTTACC TGACACTCGT CTTGCACTTC TGCCTGCGAA ATCTTTGTGT 
TACAATGACT GTCCATGGAT TAAGGTTAAA GACACAACCG TTAAATATTG TCATGGTGAT 
ATTCCAAGAG AAGTTGCAGT AAAGCTTGGA GCAATCCCAA AACGTCATAA AGCTTTAGAA 
AGATATGCAT CCAATATCTG TTTTACTACC CTTGGAACAG AGTTTGGCCA GAAAGAAAAA 
TTGACAAGTA GAATTAAAAG CATTCTTAAT GCCTACCCGT CAGAAAAAGA AATGCTGAAA 
GAACTCCTTC AGAATGCAGA TGATGCAAAA GCTACAGAGA TCTGTTTTGT GTTTGATCCT 
AGGCAGCATC CAGCTGATAG AATATTTGAT GAGAAATGGG CACCACTTCA AGGACCAGCA 
CTGTGTGTTT ACAACAACCA GCCTTTTACA GAAGATGATA TTCGAGGAAT TCAGAACCTT 
GGAAAAGGTA CAAAAGTAGG AAATCCCTGT AAAACTGGAC AATATGGTAT AGGTTTCAAT 
TCTGTTTATC ACATTACTGA TTGCCCTTCT TTCATATCTG GCAATGATAT ACTTTGTATT 
TTTGATCCTC ATGCTAGATA TGCACCAGGT TCAACATCAA CAAGTCCTGG CCGCATGTTT 
AGAGATTTAG ATGCAGATTT CAGAACACAG TTCTCAGATG TGCTGGACCT TTACTTAGGA 
GATCACTTTA AACTGGATAA TTGCACAATG TTTAGGTTTC CTCTTCGAAA TGGAGAAATG 
GCAAAAGTAT CAGAAATTTC CTCAGTTCCA TGTTCGGATA GAATGGTCCA AAACCTATTA 
GATAAGCTGC GTACAGATGG AGCAGAACTC TTAATGTTTT TGAACCATAT GGAAAAAATT 
TCTATTTGTG AGATAGAAAA AACAACAGGA GCACTGAATG TGTTGTATTC TGTGCAAGGC 
AAAATCACTG ATGGAGACAG ACTGAAACGA AAGCAATTCC ATGCATCTGT AATTGACAGC 
GTAAGTAAAA AAAAGCAGCT AAGTGAAATA CCCGTGCAAC AGATTACTTA CACAATGGAT 
ACTGAAGACT CTGAAGGAAA TCTTACAACT TGGTTAATTT GTAACAGGTC AGGCTTTTCT 
GCCATGGAAA AGGTATCCAA AAGTGTGGTT TCAGCCCACA AGAATGAGGA CATTACTCTT 
TTTCCACGTG GCGGGGTAGC AGCTTGCATT ACGCATAATT ACAAAAAGCC CCACAGAGCG 
TTCTGTTTCT TACCGTTATC GTTAGAAACT GGATTACCCT TTCACGTGAA TGGACACTTT 
GCCCTGGATT CTGCAAGAAG AAATCTGTGG CGTGATGATA ACGGTGTTGG AGTAAGAAGT 
GACTGGAATA GTAGCCTAAT GACAGCGCTG ATTGCACCGG CCTACGTTGA ACTGCTGATT 
CAGCTGAAGA AACGGTATTT CCCAGGCACT GATCCTACAG TGTCCGTGCT GCAAAACACA 
CCTGTTCATG TTGTGAAAGA CACTTTAAAA AAATTTTTAT CCTTTTTTCC AGTTAATAGA 
CTTGATATTC AGCCGGATTG GTATTGCTTA GTGAAAGCAG TTTACAGTTG CATTTATGAA 
GATCTGAAGC GTCTTTTACC TGTTATGCGA GCTCCAAATA TCGATGGTTC TGATTTGCAT 
TCTGCTGTTA TCATCACATG GGTTAATATG TCTACAGTGA ACAAAGGCAG GCCATTCTTT 
GACAATTTAC TACAAGATGA ATTGCAACAT CTCAAAAATA CAGAATACAA CATCACAACG 
CGGAAGACAG TGGCAGAAAA TGTCTACAGA CTCAAGCATT TACTCTTAGA AATTGGATTT 
AATTTGGTAT ATAACTGTGA TGAAACTGCA AATCTTTACC ACTGTCTGGT AGATGCAGAT 
ATTCCTGTCA CCTATGTGAC ACCTGCTGAT GTCCGTTCAT TTTTGATGAC ATTTTCTTTT 
CCAGACTCTA ATTGCCACAT TGGAAAGTTA CCCTGTCGTC TTCAGCAGAC GAATTTGAAA 
CTTTTCCATA GTCTCAAACT TCTGGTTGAC TACTGTTTTA AAGATGCAGA AGAAAATGAA 
ATCCAGATTG AGGGCTTGCC ACTCCTTATT ACTCTTGACA ATGTTTTGCA AATTTTTGAT 
TCAAAGCGAC CAAAGTTCTT AACAACATAC CATGAGCTGA TTCCATCTCG CAAGGATCTC 
TTTATGAACA CGTTGTACTT GAGATACAAC AATATTTTAC TGAGCAGTGA AGTAGCGAAA 
GTCTTTGATA TCAAAAGTTT TGCTGAATTG TTATCATCTG TGTTGCCGAG AGAATATAAA 
ACCAGAAATT GTATGAAATG GAAGGAAAAC TTTGCAAGTG AGTCTTGGCT TAAAAACGCC 
TGGCACTTTA TTAGTGAGTC TATAAATATA AAGGAAGAGC AAGAAGATAC GCAAGCAAAA 
TTCGATGATG TTGTTGAAAC TTTGAAGGAC TGGACTTTGC TCCCAGGTGT AAGGTTTACT 
GTCTCAGCTA ATCACCTTGT TGTGCCAGAG TGTGACGTTC TGCTGCCACT CAGCATTATG 
CATATAGCTG TTTTTCCAAA TGCTCAGAGT GATAAAGTCT TCCATGCTTT AATGAAAACA 
GGCTGTATTC AGTTGGCATT GAACAAAATT TGCTCCAAAG ACAGTGCTTT AGTGCCTTTG 
TTGTCAGGAT GTACAGCAAA TATAGATAAT CCTTCAAGCA TTCTGAAAGC CATACAATAT 
ATGGTACAGA CATCAACTTT CAGGACTGAA AAGTTAGCAG AAAGTGACTT TGAGGCTCTC 
TTAATGTACT TCAATTGCAA TTTATGTCAT TTGACATCTC AGGATGACAT TAAAATTTTA 
AAGTCTCTTC CATGTTATAA ATCGATCAGT GGCCGATACA TCAGCATTTC AAAATACGGG 
ACGTGTTACG TTCTCACGAA AAGCATCCCA TCAGTAGAAG TAGACAGATG GACACAGTCA 
ACTTCATCAG CTTTTCTTGA AGAGAAGGTT CATTTGACAG ATTTGTACAC TCTGCTTGGC 
TGTGTCTCTG TGGATGATCT TGAAGTGTAC TTGAAACATC TTTTGCCAAA AATTGAGAGC 
CTGTCTTACG ATGCAAAATT AGAACACTTG ATCTACCTAA AGAATAGGCT AACTAGCATT 
GAGGAATCTT CAGAAATGAA AGAACAGCTG TTTGAAAAGC TTGAAAGCAT TTTGATCATT 
CATGATGCAA CCAATAGATT AAAGCCTGCA AAATATTTTT ATGACAGGAC TGTAAAAGTC 
TTTGAAGTTA TGCTTCCTGA AAAGTTCTTT ATACCTCAGG ATTTTTTTAA GAAACTCGAG 
CAACTTACAA AACCAAAAAA CCAAGCTGCA TTCCTTCCAT CATGGGTAAC TTTTCTACGA 
CATATCGGAT TGAAATTCAT CATTTCGCAG CAACAGTTGC TACAGTTTGC TAAGGAGATC 
AGCATGCGAG CGAATACAGA AAACTGGTCT AAAGAAACAC TACAGAATAC TGTCGATGTC 
CTCCTTCATC ACATATTTCA AGAAAGAACT GACCTTTTGT CAGGAAATTT TCTGAAAGAA 
TTGTCATTAA TTCCTTTTCT GTGCCCTGAA CGAGCTCCAG CAGAATTTGT TAGATTTCAT 
CCTCAGTATC AAGAAGTTAA TGGGACACTT CCTCTTATAC GATTCAATGG GGCACAGGTG 
AATCCTAAAT TCAAACAGAC AGATGTGTTG CAGTTGCTGT GGACTTCATG TCCCATTCTT 
CCTGAGAAAG CAACACCTTT AAGCATCAAG GAGCAAGAGG GAAGTAGTCT TGGTGCACAA 
GAACAACTTG AGCAAGTTTT AACTATGCTG AATGTCAATT TGGACCCTCC CCTTGACAAA 
GTTATCAATA ACTGCAGGAA CATATGCAAT ATAACGACTT TGGATGAGGA CATGGTGAAA 
ACTAGGGCAA AGGTCTTAAG AAGCATTTAT GAATTTCTTA GTACAGAGAA AAGGGAATTC 
CGCTTTCAAC TTCGAGGAGT TTCTTTTGTA ATGGTGGAAG AAGGTTGGAA GCTTCTGAAG 
CCTGAAGAGG TGGTAATAAA CCTTGAGTAT GAATCTGATT TTAAACCTTA TCTTTACAAA 
CTTCCTTTAG AACTTGGTAC TTTCCATCAG TTATTTAAAC ATTTGGGTAC TGAAGATATT 
ATTTCAACAA AGCAGTATGT TGAAGTTCTA GGTCGCATAT ACAAGAGCTC GGAAGGAAAG 
CAATTGGATC CTAATGAAAT GCGCACGGTT AAAAGAGTAG TTTCTGGCCT GTTCAAAAGT 
CTTCAGAATG ATTCTGTCAA GGTTAGGAAT GACCTTGAGA ATATGAGGGA TTTTGCCCTT 
TACCTTCCGA GTCAAGATGG TAGGTTGGTA AAGTCTAGTA TCCTAGTTTT TGATGATGCA 
CCTCACTACA AAAGCAGAAT CCAAGGTAAC ATTGGTGTGC AAATGCTTGT TGATCTTAGC 
CAGTGTTACT TAGGTAAAGA CCATGGCTTT CACACTAAAT TGATTATGTT ATTCCCTCAG 
AAATTGAGGC CTCGCTTACT TAGCAGTATT CTCGAAGAAC AGTTGGATGA AGAGTCTCCC 
AAAATCTGTC AGTTTGGAGC ATTATGTTCT TTGCAGGGAA GGTTACAGTT ACTGTTGTCT 
TCGGAACAAT TCATCACAGG ACTCATTAGG ATTATGAAGC ATGAAAATGA CAATGCTTTT 
TTAGCTAATG AGGAAAAAGC AGTAAGACTT TGCAAAGCTT TGCGAGAAGG CTTGAAAGTT 
TCCTGTTTTG AGAAGTTGCA AACAACGTTA CGAGTTAAAG GCTTTGCTCC TATTCCCCAC 
AGCAAAAGCG AAACATTTGC TTTCCTGAAG AGATACGGAA ATGCAGTAAT ACTGCTTTAC 
ATACAGCATT CTGACAGCAA AGACATAAAT TTCCTGTTGG CATTAGCCAT GACCTTAAAA 
TCTGCAACTG ACAACCTGAT TTCTGATACC TCATATTTAA TTGCCATGCT GGGTTGTAAT 
GATATTTACC GGATCAGTGA AAAGCTTGAC AGTTTAGGAG TGAAGTACGA CTCTTCTGAG 
CCATCAAAAC TTGAACTACC AACACCTGGC ACTCCTATAC CAGCTGAAAT TCACTATACA 
CTTCTTATGG ATCCAATGAA TGTATTTTAC CCTGGTGAAT ATGTTGGTTA CCTGGTTGAT 
GCTGAAGGTG GTGATATATA CGGATCATAT CAACCAACTT ACACCTATGC AATCATTGTA 
CAAGAAGTGG AAAGAGAAGA TGATGAAAAT CCCAGTTTTC TAGGGAAGAT TTACCAGATC 
GATATTGGAT ACAGTGAGTA TAAAATAGTG AGCTCTCTTG ACTTGTACAA ATTTTCAAGA 
CCGGAGGAAA GTTCTCAAAG CAGGGATAGC ACACCTTCTA CCCCAACCAG TCCTACAGAA 
TTTCCAGCAC ACGGACCTAG GGCCATTCCA CCTCTCTTCG CTGGTAGAGA GAGCCACAAA 
ACAATGTCCT CAAAACATCA TTCTCCAAAA AAGATAAAGT CAAACTCATT GCCAGAGATA 
TTAAGAGAAG TTACATCTGT GATTGAACAG GCTTGGAAGC TTCCAGAATC TGAAAGAAAA 
AAGATTATTA GGAGGCTGTA TCTTAAATGG CATCCAGATA AAAATGCAGA GAATCTTGAT 
ATAGCTAATG AAGTTTTCAA ACATTTACAG AATGAGATTA ACAGGCTAGA GAAACAGGCC 
TTCATGGATC AAAATATGGA CAGAGCCTCG AGAAGACCAT TTTCATCCTC TGCTTCTCGA 
TTTCAGTCGG ATAAATTTTC ATTTCAAAGA TTTTATACTT CATGGAATCA AGAAGCAACA 
AGCCACAAAT CTGAAAGGCA GCAGTATAAA GAAAAGTGTC CATCTTCCAC GGCGCCATCT 
TACTCGCAGC GCTTTTTTGT CCCACCTACC TTCAGGTCCG TTGGCAATCC TGTAGAGGCA 
CGTCGGTGGC TTAGGCAGGC CCGAGCTAAC TTCTCAGCTG CAAGAAATGA CCTTCATAAA 
AATGCCAATG AATGGGTATG CTTTAAGTGC TACCTTTCTA CTAAACTCGC CTTGATTGCA 
GCTGACTATG CTGTGAAGGG CAAATCGGAC AAAGACGTAA AACCAGCAGC CCTTGCACAA 
AAAATAGAGG AATACAGTCA GCAACTTGAA GGGCTTACAA ATGATGTTCA GACGTTGGAA 
GCTTACGGAG TAGATAGCTT AAAAACTAGG TATCCTGACT TACTGCCTTT CCCACAGATT 
CCCAATGATA GATTTACTTC AGAAGTTGCT ATGAGAGTGA TGGAATGTAC TGCTTGTATC 
ATAATAAAAC TTGAGAACTT TATACAACAG AAGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821328.1
CDS	267..13565
Translation	MAQYQGPAFYAYNDAIFTPEDWHGIQEIARSRKKDDPLKVGRFGIGFNSVYHITDVPSIFSGDQIGMLDPHQTLFGPHESGQCWNLKEDSKEINELTDQFAPFIGVFGSTKETFKNGSFPGTFFRFPLRLQPSQLSSNVYDKQKVLELFESFRADADTVLLFLKSVQDVSLHVREADGTERLIFRVTASENKALKHERPNSIKILGTAINQYCKGVPSNSITCVTYHVNIVVEDESVKDAQKTSWLVCNCVGGRGMCTELDSLADDLKFVPTIGIAMSLSTNEEEKGAVADFSGRAFCFLPLPPGEESKTGLPVHVSGFFGLTDNRRSIKWRELDQWRDPAALWNDLLVVNIVPKAYTTLILEAIKRMETEKNSDFPLSAERIYRLWPDENKIRVPWKPIVVPLFKELLQHTVIYSVSNQWIQVEQVHFSEMDESLEYTQSVLNYLQNSGKQIAKVPANIASAVHLTISAAKAVKKVTPAVVRQVLRKSGHSGTAEEKLHLLEFVLSDGVYSELIGLELLPLQNGNFIPFSSSVSEQDVVYITSEEYPRSLFPGLEGRLLSDDLKPEVLAALKEAAKSRGRPCTQLQLLNPERFARLIKEVMNSTWPGRDMVVQWHPGLEDKNHPSVSWLKMVWKNLYIHFSDDLSVFDDMPLIPKTLLEENQTSVELVRLRNPSPIILEDESETQLPEYLADIIQKVGGVVLKQLDISIQHPLIKKYVHPPLPSAVLQIMEKMSLQKLCSQVASFPSTHKDALRAFLASLTDVSEKERRIIQELLIFKKIEKSSDESVPVFTGLKGSKVLHHTAKIPPGLRFSVPIIDSSDEATIRLANLLKIEQLKSTDCLKFVIQDIRSDFYSYDEITQIMQWVLENLTFLKNENTDVIDWLTSLKFIKISEENIMSASELFDPEVEVLQNLFYAEEEICFPPVVFTSSDILHSLRQIGLKNEANLEESDIMRVANKIESLHANSNTDHDLLLRKARTLVMVLNKNHTLLHSSETKAALKKIKWIPACKERPPNYPGSLVWKGDHCNLCSPPEMCDIAHAILVGSSVPLVENVLLDTEKALGIPTKPSIKAVLKHFKVVVDWHSSKTFSDEDYYQFQHILLEIYGFMHDHLEEGKEAFKALKFPWVWTGKTFCSLTQAVIKSVPDLDLQPYLHYVPKTMAKFHQLFICCGSIEQLTPDHVSMVIQKIYLKSEQPLSEQESKQNLHIMLSIIRWLYSNQIPASLHTPMPLYSGKLPYKLAMRPIHECCYCDIKVDDLNDLLEDSVEPIILVHEDIPMKTAEWLNVPCLSTRLINPENMGFEQSGQREPLTVRIKNILEEYPSISDIFKELLQNADDANATECNFLIDMRRNMDIRENLLDPGMAACHGPALWSFNNSEFSDSDFLNITRLGESLKRSEVDKVGKFGLGFNSVYHITDVPIILSREFMIMFDPNVNHISKHIRDKSNPGIKINWSKQQKRLRKFPNQFKPFIGVFGCQLPLTVESPYSYKGTLFRLSFRTQQEAKVSEVSSASYNTADIYSLVDEFSICGHRLIIFAQSVNSMVLKYLKIEADDPGVAQDVVTIKKNLCSSKALTAPNVSVLKEAAKLMKVCNGSSKKLPTETPKSSCILQIVVEEFHHVFRRIADLQSPLFRGTEDDPASFFEMAKSGQMKRSADELPQKTVDSTTWLICSCMDTGDALKFSLHESGRRLGLVPCGGVGVLLSETQDQKWIVKPNCNNIGEVFCYLPLRIKTGLPLHINGCFAVTSNRKEIWKTDTKGRWNTVFMRHVIVKAYIEALCVLRDMAINGELVDYSYSAVWPDPDSVHDDFSVICQGFYEDIAHMKNKEGIKVFSDGFSWVSMKNVRFLDDSILKRPDIGPSAFKIFQKYLKKTGSKNLCAVDLPSWVKTGFEEAGCKYILLENTFSEKQFFSEVFFPNIQEIDAELRDPLMRYVLNEKVEEFSGILRVTPCIPCSLDGHPLVTPSRLIHPEGRVAKLYDAEDGRFPYGTTQDYLNPVILVKLVQLGMAKDDILWEDLMERAESVAEVNKIDHAAACLRSSIIVSLIDEKLKSRDPRAKEFAAKCQTIPFLPFLSKPAGFSLHWKGSDFQPEAMFSATDLFTADHQDTVCLIQPILNENSHSFKGCGALSLAVKEFLGLLKKPAVNLVINQLEEVAKSFDGITLYQENITNACYKYLHEAMLQNESTKAMIIEQLTNCSFILVENVYVDPTKVSFHLNFEAAPYLYQLPNKYKNSFRELFESVGVRQAFTVEDFAVVLELIDQERGNKQLTEDNFQLCRRIISEGIWGLIREKKQEFCEKKYGEILLPDTRLALLPAKSLCYNDCPWIKVKDTTVKYCHGDIPREVAVKLGAIPKRHKALERYASNICFTTLGTEFGQKEKLTSRIKSILNAYPSEKEMLKELLQNADDAKATEICFVFDPRQHPADRIFDEKWAPLQGPALCVYNNQPFTEDDIRGIQNLGKGTKVGNPCKTGQYGIGFNSVYHITDCPSFISGNDILCIFDPHARYAPGSTSTSPGRMFRDLDADFRTQFSDVLDLYLGDHFKLDNCTMFRFPLRNGEMAKVSEISSVPCSDRMVQNLLDKLRTDGAELLMFLNHMEKISICEIEKTTGALNVLYSVQGKITDGDRLKRKQFHASVIDSVSKKKQLSEIPVQQITYTMDTEDSEGNLTTWLICNRSGFSAMEKVSKSVVSAHKNEDITLFPRGGVAACITHNYKKPHRAFCFLPLSLETGLPFHVNGHFALDSARRNLWRDDNGVGVRSDWNSSLMTALIAPAYVELLIQLKKRYFPGTDPTVSVLQNTPVHVVKDTLKKFLSFFPVNRLDIQPDWYCLVKAVYSCIYEDLKRLLPVMRAPNIDGSDLHSAVIITWVNMSTVNKGRPFFDNLLQDELQHLKNTEYNITTRKTVAENVYRLKHLLLEIGFNLVYNCDETANLYHCLVDADIPVTYVTPADVRSFLMTFSFPDSNCHIGKLPCRLQQTNLKLFHSLKLLVDYCFKDAEENEIQIEGLPLLITLDNVLQIFDSKRPKFLTTYHELIPSRKDLFMNTLYLRYNNILLSSEVAKVFDIKSFAELLSSVLPREYKTRNCMKWKENFASESWLKNAWHFISESINIKEEQEDTQAKFDDVVETLKDWTLLPGVRFTVSANHLVVPECDVLLPLSIMHIAVFPNAQSDKVFHALMKTGCIQLALNKICSKDSALVPLLSGCTANIDNPSSILKAIQYMVQTSTFRTEKLAESDFEALLMYFNCNLCHLTSQDDIKILKSLPCYKSISGRYISISKYGTCYVLTKSIPSVEVDRWTQSTSSAFLEEKVHLTDLYTLLGCVSVDDLEVYLKHLLPKIESLSYDAKLEHLIYLKNRLTSIEESSEMKEQLFEKLESILIIHDATNRLKPAKYFYDRTVKVFEVMLPEKFFIPQDFFKKLEQLTKPKNQAAFLPSWVTFLRHIGLKFIISQQQLLQFAKEISMRANTENWSKETLQNTVDVLLHHIFQERTDLLSGNFLKELSLIPFLCPERAPAEFVRFHPQYQEVNGTLPLIRFNGAQVNPKFKQTDVLQLLWTSCPILPEKATPLSIKEQEGSSLGAQEQLEQVLTMLNVNLDPPLDKVINNCRNICNITTLDEDMVKTRAKVLRSIYEFLSTEKREFRFQLRGVSFVMVEEGWKLLKPEEVVINLEYESDFKPYLYKLPLELGTFHQLFKHLGTEDIISTKQYVEVLGRIYKSSEGKQLDPNEMRTVKRVVSGLFKSLQNDSVKVRNDLENMRDFALYLPSQDGRLVKSSILVFDDAPHYKSRIQGNIGVQMLVDLSQCYLGKDHGFHTKLIMLFPQKLRPRLLSSILEEQLDEESPKICQFGALCSLQGRLQLLLSSEQFITGLIRIMKHENDNAFLANEEKAVRLCKALREGLKVSCFEKLQTTLRVKGFAPIPHSKSETFAFLKRYGNAVILLYIQHSDSKDINFLLALAMTLKSATDNLISDTSYLIAMLGCNDIYRISEKLDSLGVKYDSSEPSKLELPTPGTPIPAEIHYTLLMDPMNVFYPGEYVGYLVDAEGGDIYGSYQPTYTYAIIVQEVEREDDENPSFLGKIYQIDIGYSEYKIVSSLDLYKFSRPEESSQSRDSTPSTPTSPTEFPAHGPRAIPPLFAGRESHKTMSSKHHSPKKIKSNSLPEILREVTSVIEQAWKLPESERKKIIRRLYLKWHPDKNAENLDIANEVFKHLQNEINRLEKQAFMDQNMDRASRRPFSSSASRFQSDKFSFQRFYTSWNQEATSHKSERQQYKEKCPSSTAPSYSQRFFVPPTFRSVGNPVEARRWLRQARANFSAARNDLHKNANEWVCFKCYLSTKLALIAADYAVKGKSDKDVKPAALAQKIEEYSQQLEGLTNDVQTLEAYGVDSLKTRYPDLLPFPQIPNDRFTSEVAMRVMECTACIIIKLENFIQQKV

Target ORF information:

RefSeq Version	XM_014965842.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sacsin molecular chaperone (SACS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965842.1

ORF Insert Sequence:

ATGGCGCAAT ATCAGGGGCC AGCATTTTAT GCATACAACG ATGCAATTTT CACCCCTGAG 
GACTGGCATG GTATACAGGA AATAGCAAGA AGCAGGAAGA AAGATGATCC CCTGAAAGTT 
GGAAGATTTG GAATTGGCTT TAATTCAGTT TATCATATAA CAGATGTCCC TAGCATTTTC 
AGTGGTGACC AGATTGGAAT GCTTGATCCC CATCAGACCC TTTTTGGACC TCATGAATCA 
GGCCAGTGCT GGAATCTAAA AGAAGATAGC AAGGAAATCA ATGAACTTAC AGACCAGTTT 
GCTCCGTTCA TTGGTGTGTT TGGCAGCACT AAGGAAACGT TTAAGAATGG AAGCTTTCCT 
GGTACCTTTT TTCGTTTCCC ACTTCGTCTG CAGCCTTCCC AGCTGAGCAG CAATGTTTAC 
GACAAACAGA AGGTTCTGGA GCTGTTTGAA TCCTTCAGAG CAGATGCAGA CACAGTATTG 
CTCTTTCTGA AGAGTGTTCA GGATGTGTCA TTGCATGTTA GGGAAGCTGA TGGAACTGAG 
AGGCTGATTT TTAGAGTAAC TGCTAGTGAG AACAAGGCCT TGAAACATGA AAGACCAAAT 
TCTATCAAGA TCCTAGGAAC TGCAATAAAT CAGTATTGTA AAGGAGTTCC AAGCAATAGC 
ATAACATGTG TGACATACCA TGTAAATATA GTTGTAGAAG ATGAGAGCGT TAAGGACGCA 
CAGAAAACAT CTTGGTTGGT GTGTAATTGT GTGGGTGGTC GAGGAATGTG TACTGAACTG 
GATAGTTTAG CTGATGACTT GAAATTTGTT CCTACTATTG GAATAGCCAT GTCTTTATCA 
ACTAATGAGG AGGAAAAAGG AGCTGTAGCA GACTTTTCAG GAAGAGCGTT TTGTTTTCTG 
CCTTTGCCTC CAGGTGAAGA GAGCAAAACT GGACTCCCAG TTCATGTCAG TGGTTTCTTT 
GGTCTCACGG ACAATAGGAG GAGTATCAAA TGGCGAGAGC TGGATCAATG GAGAGATCCA 
GCAGCTTTGT GGAATGATCT TCTCGTCGTG AATATTGTGC CCAAGGCATA CACCACCCTT 
ATTTTGGAAG CTATCAAACG AATGGAGACT GAGAAGAATT CAGATTTTCC ATTGTCAGCT 
GAACGAATTT ATAGGTTATG GCCTGATGAG AACAAAATCA GGGTACCTTG GAAACCAATT 
GTAGTACCTC TCTTTAAAGA GCTGCTCCAG CATACAGTTA TTTACTCAGT GAGTAATCAG 
TGGATACAGG TTGAACAGGT GCACTTTTCT GAAATGGATG AGAGTTTGGA GTACACACAG 
TCTGTTCTCA ACTACCTCCA GAATTCAGGG AAGCAGATTG CCAAGGTACC AGCAAATATT 
GCTAGTGCAG TGCATCTTAC TATTTCTGCT GCTAAAGCTG TGAAAAAAGT GACTCCTGCT 
GTAGTACGGC AAGTACTAAG GAAATCTGGA CACAGTGGAA CTGCTGAAGA GAAGCTTCAT 
CTTCTAGAAT TTGTGCTTTC TGATGGAGTC TACAGTGAAC TGATTGGACT GGAGCTTTTG 
CCATTACAAA ATGGAAATTT TATTCCTTTT TCCTCATCTG TGTCAGAACA GGATGTAGTT 
TACATAACCT CAGAAGAATA TCCCAGATCA CTCTTTCCTG GCCTGGAAGG AAGGTTACTT 
TCTGATGATC TGAAACCTGA GGTTCTAGCT GCCTTGAAAG AAGCTGCCAA AAGCCGAGGA 
AGGCCATGCA CTCAGCTGCA GCTTCTGAAC CCAGAAAGAT TCGCTCGGCT CATTAAAGAA 
GTTATGAATA GCACGTGGCC TGGCAGAGAC ATGGTGGTAC AGTGGCATCC AGGTTTGGAA 
GACAAAAATC ACCCATCAGT TTCCTGGCTT AAGATGGTCT GGAAGAACCT ATATATACAT 
TTTTCTGATG ACTTGAGTGT GTTTGATGAC ATGCCGCTTA TTCCCAAGAC ACTGCTGGAG 
GAAAACCAAA CATCAGTGGA ACTGGTTAGA CTTAGGAATC CATCACCTAT CATTCTGGAA 
GATGAATCTG AGACTCAGCT CCCGGAATAT TTAGCTGATA TTATTCAGAA AGTTGGTGGA 
GTTGTACTTA AGCAGCTAGA TATTTCTATC CAACATCCGC TTATAAAAAA ATACGTGCAT 
CCACCATTAC CAAGTGCTGT CTTACAAATC ATGGAAAAAA TGTCCTTACA GAAACTATGC 
AGTCAGGTTG CATCATTCCC GTCAACACAT AAAGATGCTC TTAGGGCATT CTTGGCTAGT 
TTAACTGATG TAAGTGAAAA AGAGAGAAGA ATTATTCAGG AATTGCTAAT ATTTAAAAAA 
ATTGAAAAAT CATCTGATGA AAGTGTCCCT GTTTTTACTG GGCTAAAAGG TAGTAAAGTA 
TTACACCACA CTGCCAAAAT TCCTCCTGGT CTAAGATTTT CTGTTCCAAT AATTGACAGC 
AGTGATGAAG CTACTATTCG CTTAGCTAAC CTGCTAAAAA TAGAACAGCT AAAGAGCACA 
GATTGCTTGA AGTTTGTTAT ACAGGACATA AGAAGTGATT TTTATTCATA TGATGAAATA 
ACACAGATAA TGCAATGGGT TCTTGAAAAT CTGACTTTTC TGAAAAATGA GAATACAGAT 
GTGATAGATT GGCTGACATC ACTAAAGTTT ATTAAGATTT CAGAGGAAAA TATTATGTCA 
GCAAGCGAAC TCTTTGATCC TGAGGTAGAA GTTCTGCAAA ACTTATTTTA TGCTGAGGAG 
GAAATTTGCT TCCCTCCTGT TGTCTTTACA TCTTCAGATA TTCTTCATTC TCTGAGACAA 
ATAGGCTTAA AAAATGAGGC CAATCTTGAG GAGAGTGATA TTATGAGAGT GGCAAATAAA 
ATTGAAAGTT TGCATGCTAA CTCTAACACT GATCATGATC TACTGTTAAG AAAAGCTAGA 
ACTCTCGTGA TGGTTTTGAA CAAAAACCAT ACACTGCTTC ACTCATCTGA AACAAAAGCT 
GCACTGAAAA AAATAAAATG GATCCCGGCA TGTAAGGAAA GACCTCCCAA TTATCCAGGG 
TCCTTAGTTT GGAAAGGAGA CCATTGCAAT CTGTGTTCAC CACCAGAAAT GTGTGATATA 
GCTCATGCAA TTTTAGTTGG TTCCTCAGTT CCTCTTGTGG AAAATGTGCT GTTGGATACA 
GAAAAAGCCC TCGGCATCCC CACCAAGCCC AGCATAAAAG CAGTCTTAAA ACATTTCAAG 
GTGGTGGTTG ATTGGCACAG TTCTAAAACC TTCAGTGATG AGGACTACTA TCAGTTTCAG 
CATATCTTGC TTGAGATTTA TGGATTTATG CATGATCATC TAGAAGAAGG CAAAGAAGCT 
TTTAAAGCCC TGAAATTTCC CTGGGTTTGG ACAGGTAAAA CATTTTGTTC CCTCACACAA 
GCAGTAATAA AGTCAGTACC TGATCTCGAT CTCCAACCTT ATCTACATTA TGTGCCAAAA 
ACGATGGCGA AGTTTCACCA GTTGTTTATA TGTTGTGGTT CAATAGAGCA GTTAACACCA 
GACCACGTGT CTATGGTCAT TCAGAAAATA TATCTGAAAA GCGAGCAGCC TCTCTCGGAA 
CAAGAGAGTA AACAAAATCT TCACATTATG TTGAGCATCA TAAGGTGGCT GTACAGCAAT 
CAGATTCCTG CAAGTCTGCA CACGCCCATG CCGCTCTACA GTGGCAAACT TCCCTACAAA 
CTTGCCATGA GGCCCATTCA CGAGTGCTGT TACTGCGACA TCAAAGTGGA TGACTTAAAC 
GATTTGCTGG AAGATTCTGT TGAACCGATA ATTTTAGTGC ATGAAGACAT ACCGATGAAA 
ACCGCTGAGT GGTTAAATGT CCCATGCCTT AGCACAAGAC TGATTAATCC AGAAAATATG 
GGATTTGAGC AGTCTGGGCA AAGAGAGCCT CTGACTGTAA GAATTAAAAA CATTTTGGAG 
GAATACCCTT CCATTTCAGA TATCTTTAAA GAGCTTCTTC AAAATGCTGA TGATGCTAAT 
GCAACGGAAT GCAATTTCCT GATTGACATG AGAAGAAATA TGGATATAAG AGAGAATCTT 
CTGGATCCAG GAATGGCAGC ATGTCATGGT CCAGCTTTGT GGTCATTCAA TAATTCTGAA 
TTTTCTGACT CTGATTTCCT AAATATAACT CGATTAGGAG AGTCTTTAAA AAGGAGTGAA 
GTTGACAAAG TAGGAAAATT TGGACTGGGA TTCAATTCTG TCTATCATAT CACTGATGTT 
CCTATTATTT TGAGCCGGGA GTTCATGATT ATGTTCGATC CAAACGTTAA CCATATCAGT 
AAGCATATTA GAGATAAATC TAACCCTGGC ATCAAAATCA ACTGGAGTAA ACAACAGAAG 
AGGCTGCGAA AGTTTCCCAA CCAGTTTAAG CCTTTTATAG GAGTTTTTGG TTGCCAGTTG 
CCACTGACTG TAGAATCTCC TTACAGCTAC AAAGGAACTC TTTTCAGGCT GTCCTTTAGA 
ACTCAGCAGG AAGCTAAAGT GAGTGAAGTC AGCAGTGCGT CTTACAATAC AGCAGACATT 
TACTCTCTTG TGGATGAGTT TAGCATCTGT GGTCATAGAC TGATAATATT TGCTCAGAGT 
GTAAATTCAA TGGTTTTGAA ATACTTGAAA ATTGAAGCAG ATGATCCTGG GGTGGCACAG 
GATGTTGTCA CTATCAAAAA AAATCTGTGT TCTTCCAAAG CCTTGACTGC CCCAAACGTA 
AGTGTTTTAA AGGAAGCGGC TAAACTCATG AAGGTCTGCA ATGGCAGTAG TAAAAAACTT 
CCCACTGAAA CACCAAAGTC CTCGTGTATC TTACAGATAG TAGTAGAAGA ATTTCACCAT 
GTATTTAGAC GGATTGCTGA TTTACAGTCT CCACTTTTCA GAGGTACAGA AGATGATCCA 
GCTTCTTTCT TTGAAATGGC TAAATCAGGA CAGATGAAGA GATCAGCTGA TGAATTGCCA 
CAGAAAACAG TAGATTCAAC AACATGGCTT ATATGTTCTT GCATGGACAC TGGAGATGCT 
TTAAAATTTT CATTACATGA AAGTGGACGA AGACTAGGTC TTGTTCCCTG TGGTGGAGTA 
GGAGTGTTGC TCTCTGAAAC TCAGGATCAA AAATGGATTG TGAAACCTAA TTGCAACAAC 
ATAGGTGAAG TATTCTGCTA TTTACCTCTA CGAATCAAAA CAGGCTTACC TCTTCATATA 
AATGGCTGCT TTGCAGTTAC TTCAAATCGG AAAGAAATCT GGAAAACGGA CACAAAGGGA 
AGATGGAATA CAGTATTCAT GAGGCATGTT ATTGTAAAAG CCTACATAGA AGCCCTTTGT 
GTTCTGCGTG ACATGGCAAT CAATGGTGAG CTTGTTGACT ACAGTTACTC TGCAGTGTGG 
CCAGATCCTG ATTCAGTTCA TGATGATTTT TCTGTTATTT GTCAAGGGTT TTATGAAGAC 
ATAGCTCACA TGAAGAACAA AGAAGGGATC AAAGTGTTTT CTGATGGTTT CTCTTGGGTT 
TCCATGAAGA ATGTAAGGTT TTTAGATGAC TCTATACTGA AACGACCAGA TATTGGACCA 
TCAGCCTTTA AGATCTTTCA GAAGTACCTT AAAAAAACTG GTTCTAAAAA TCTGTGTGCT 
GTAGATCTTC CTTCCTGGGT AAAGACAGGA TTTGAAGAAG CAGGATGCAA GTATATATTA 
CTGGAGAACA CTTTTTCTGA GAAACAGTTC TTTTCTGAGG TGTTTTTCCC TAACATTCAG 
GAGATTGATG CAGAGCTGCG TGATCCTTTG ATGCGTTATG TTCTCAATGA AAAAGTTGAG 
GAGTTCTCAG GAATTCTTCG TGTTACTCCG TGTATTCCCT GTTCGTTGGA TGGACATCCA 
TTAGTTACAC CATCAAGACT GATCCATCCT GAAGGAAGAG TTGCAAAATT ATATGATGCT 
GAGGATGGAA GATTTCCTTA CGGCACCACT CAGGATTATC TTAACCCAGT TATTTTGGTT 
AAACTTGTTC AGTTGGGAAT GGCTAAAGAT GATATTTTAT GGGAAGATCT GATGGAACGT 
GCAGAGTCAG TAGCCGAAGT TAACAAGATT GATCATGCTG CAGCTTGTCT TAGAAGCAGC 
ATTATAGTGA GTCTTATTGA TGAAAAACTA AAATCTAGGG ACCCTAGAGC TAAAGAATTT 
GCTGCAAAAT GTCAAACCAT CCCTTTCCTT CCTTTCCTTA GCAAACCAGC AGGCTTCTCA 
CTGCACTGGA AAGGCAGTGA TTTTCAGCCT GAAGCAATGT TTTCAGCAAC TGATCTTTTC 
ACTGCTGATC ATCAAGATAC AGTTTGCCTA ATACAACCCA TTCTTAATGA AAATTCCCAC 
TCCTTTAAAG GTTGTGGTGC TTTGTCATTA GCTGTCAAAG AATTTTTGGG TTTACTGAAG 
AAACCAGCTG TTAATTTGGT CATAAATCAG TTAGAAGAAG TTGCAAAGTC ATTTGATGGA 
ATCACGTTAT ATCAAGAAAA TATCACTAAT GCTTGTTACA AATATCTACA TGAAGCAATG 
TTACAGAACG AATCAACGAA AGCTATGATA ATTGAACAAC TGACAAACTG TAGTTTTATT 
CTGGTTGAGA ATGTGTATGT TGATCCAACA AAAGTGTCTT TTCATTTGAA TTTTGAAGCA 
GCACCCTACC TCTATCAGTT GCCTAACAAA TATAAAAATA GTTTCCGTGA GCTGTTTGAA 
AGCGTGGGGG TAAGACAGGC CTTTACAGTT GAAGATTTTG CTGTAGTTCT GGAATTAATA 
GATCAGGAAA GAGGGAACAA ACAACTAACA GAAGACAACT TTCAGCTTTG CAGGAGAATA 
ATTAGTGAAG GAATATGGGG CCTAATTAGA GAGAAGAAGC AGGAATTTTG TGAGAAAAAG 
TACGGTGAGA TTTTGTTACC TGACACTCGT CTTGCACTTC TGCCTGCGAA ATCTTTGTGT 
TACAATGACT GTCCATGGAT TAAGGTTAAA GACACAACCG TTAAATATTG TCATGGTGAT 
ATTCCAAGAG AAGTTGCAGT AAAGCTTGGA GCAATCCCAA AACGTCATAA AGCTTTAGAA 
AGATATGCAT CCAATATCTG TTTTACTACC CTTGGAACAG AGTTTGGCCA GAAAGAAAAA 
TTGACAAGTA GAATTAAAAG CATTCTTAAT GCCTACCCGT CAGAAAAAGA AATGCTGAAA 
GAACTCCTTC AGAATGCAGA TGATGCAAAA GCTACAGAGA TCTGTTTTGT GTTTGATCCT 
AGGCAGCATC CAGCTGATAG AATATTTGAT GAGAAATGGG CACCACTTCA AGGACCAGCA 
CTGTGTGTTT ACAACAACCA GCCTTTTACA GAAGATGATA TTCGAGGAAT TCAGAACCTT 
GGAAAAGGTA CAAAAGTAGG AAATCCCTGT AAAACTGGAC AATATGGTAT AGGTTTCAAT 
TCTGTTTATC ACATTACTGA TTGCCCTTCT TTCATATCTG GCAATGATAT ACTTTGTATT 
TTTGATCCTC ATGCTAGATA TGCACCAGGT TCAACATCAA CAAGTCCTGG CCGCATGTTT 
AGAGATTTAG ATGCAGATTT CAGAACACAG TTCTCAGATG TGCTGGACCT TTACTTAGGA 
GATCACTTTA AACTGGATAA TTGCACAATG TTTAGGTTTC CTCTTCGAAA TGGAGAAATG 
GCAAAAGTAT CAGAAATTTC CTCAGTTCCA TGTTCGGATA GAATGGTCCA AAACCTATTA 
GATAAGCTGC GTACAGATGG AGCAGAACTC TTAATGTTTT TGAACCATAT GGAAAAAATT 
TCTATTTGTG AGATAGAAAA AACAACAGGA GCACTGAATG TGTTGTATTC TGTGCAAGGC 
AAAATCACTG ATGGAGACAG ACTGAAACGA AAGCAATTCC ATGCATCTGT AATTGACAGC 
GTAAGTAAAA AAAAGCAGCT AAGTGAAATA CCCGTGCAAC AGATTACTTA CACAATGGAT 
ACTGAAGACT CTGAAGGAAA TCTTACAACT TGGTTAATTT GTAACAGGTC AGGCTTTTCT 
GCCATGGAAA AGGTATCCAA AAGTGTGGTT TCAGCCCACA AGAATGAGGA CATTACTCTT 
TTTCCACGTG GCGGGGTAGC AGCTTGCATT ACGCATAATT ACAAAAAGCC CCACAGAGCG 
TTCTGTTTCT TACCGTTATC GTTAGAAACT GGATTACCCT TTCACGTGAA TGGACACTTT 
GCCCTGGATT CTGCAAGAAG AAATCTGTGG CGTGATGATA ACGGTGTTGG AGTAAGAAGT 
GACTGGAATA GTAGCCTAAT GACAGCGCTG ATTGCACCGG CCTACGTTGA ACTGCTGATT 
CAGCTGAAGA AACGGTATTT CCCAGGCACT GATCCTACAG TGTCCGTGCT GCAAAACACA 
CCTGTTCATG TTGTGAAAGA CACTTTAAAA AAATTTTTAT CCTTTTTTCC AGTTAATAGA 
CTTGATATTC AGCCGGATTG GTATTGCTTA GTGAAAGCAG TTTACAGTTG CATTTATGAA 
GATCTGAAGC GTCTTTTACC TGTTATGCGA GCTCCAAATA TCGATGGTTC TGATTTGCAT 
TCTGCTGTTA TCATCACATG GGTTAATATG TCTACAGTGA ACAAAGGCAG GCCATTCTTT 
GACAATTTAC TACAAGATGA ATTGCAACAT CTCAAAAATA CAGAATACAA CATCACAACG 
CGGAAGACAG TGGCAGAAAA TGTCTACAGA CTCAAGCATT TACTCTTAGA AATTGGATTT 
AATTTGGTAT ATAACTGTGA TGAAACTGCA AATCTTTACC ACTGTCTGGT AGATGCAGAT 
ATTCCTGTCA CCTATGTGAC ACCTGCTGAT GTCCGTTCAT TTTTGATGAC ATTTTCTTTT 
CCAGACTCTA ATTGCCACAT TGGAAAGTTA CCCTGTCGTC TTCAGCAGAC GAATTTGAAA 
CTTTTCCATA GTCTCAAACT TCTGGTTGAC TACTGTTTTA AAGATGCAGA AGAAAATGAA 
ATCCAGATTG AGGGCTTGCC ACTCCTTATT ACTCTTGACA ATGTTTTGCA AATTTTTGAT 
TCAAAGCGAC CAAAGTTCTT AACAACATAC CATGAGCTGA TTCCATCTCG CAAGGATCTC 
TTTATGAACA CGTTGTACTT GAGATACAAC AATATTTTAC TGAGCAGTGA AGTAGCGAAA 
GTCTTTGATA TCAAAAGTTT TGCTGAATTG TTATCATCTG TGTTGCCGAG AGAATATAAA 
ACCAGAAATT GTATGAAATG GAAGGAAAAC TTTGCAAGTG AGTCTTGGCT TAAAAACGCC 
TGGCACTTTA TTAGTGAGTC TATAAATATA AAGGAAGAGC AAGAAGATAC GCAAGCAAAA 
TTCGATGATG TTGTTGAAAC TTTGAAGGAC TGGACTTTGC TCCCAGGTGT AAGGTTTACT 
GTCTCAGCTA ATCACCTTGT TGTGCCAGAG TGTGACGTTC TGCTGCCACT CAGCATTATG 
CATATAGCTG TTTTTCCAAA TGCTCAGAGT GATAAAGTCT TCCATGCTTT AATGAAAACA 
GGCTGTATTC AGTTGGCATT GAACAAAATT TGCTCCAAAG ACAGTGCTTT AGTGCCTTTG 
TTGTCAGGAT GTACAGCAAA TATAGATAAT CCTTCAAGCA TTCTGAAAGC CATACAATAT 
ATGGTACAGA CATCAACTTT CAGGACTGAA AAGTTAGCAG AAAGTGACTT TGAGGCTCTC 
TTAATGTACT TCAATTGCAA TTTATGTCAT TTGACATCTC AGGATGACAT TAAAATTTTA 
AAGTCTCTTC CATGTTATAA ATCGATCAGT GGCCGATACA TCAGCATTTC AAAATACGGG 
ACGTGTTACG TTCTCACGAA AAGCATCCCA TCAGTAGAAG TAGACAGATG GACACAGTCA 
ACTTCATCAG CTTTTCTTGA AGAGAAGGTT CATTTGACAG ATTTGTACAC TCTGCTTGGC 
TGTGTCTCTG TGGATGATCT TGAAGTGTAC TTGAAACATC TTTTGCCAAA AATTGAGAGC 
CTGTCTTACG ATGCAAAATT AGAACACTTG ATCTACCTAA AGAATAGGCT AACTAGCATT 
GAGGAATCTT CAGAAATGAA AGAACAGCTG TTTGAAAAGC TTGAAAGCAT TTTGATCATT 
CATGATGCAA CCAATAGATT AAAGCCTGCA AAATATTTTT ATGACAGGAC TGTAAAAGTC 
TTTGAAGTTA TGCTTCCTGA AAAGTTCTTT ATACCTCAGG ATTTTTTTAA GAAACTCGAG 
CAACTTACAA AACCAAAAAA CCAAGCTGCA TTCCTTCCAT CATGGGTAAC TTTTCTACGA 
CATATCGGAT TGAAATTCAT CATTTCGCAG CAACAGTTGC TACAGTTTGC TAAGGAGATC 
AGCATGCGAG CGAATACAGA AAACTGGTCT AAAGAAACAC TACAGAATAC TGTCGATGTC 
CTCCTTCATC ACATATTTCA AGAAAGAACT GACCTTTTGT CAGGAAATTT TCTGAAAGAA 
TTGTCATTAA TTCCTTTTCT GTGCCCTGAA CGAGCTCCAG CAGAATTTGT TAGATTTCAT 
CCTCAGTATC AAGAAGTTAA TGGGACACTT CCTCTTATAC GATTCAATGG GGCACAGGTG 
AATCCTAAAT TCAAACAGAC AGATGTGTTG CAGTTGCTGT GGACTTCATG TCCCATTCTT 
CCTGAGAAAG CAACACCTTT AAGCATCAAG GAGCAAGAGG GAAGTAGTCT TGGTGCACAA 
GAACAACTTG AGCAAGTTTT AACTATGCTG AATGTCAATT TGGACCCTCC CCTTGACAAA 
GTTATCAATA ACTGCAGGAA CATATGCAAT ATAACGACTT TGGATGAGGA CATGGTGAAA 
ACTAGGGCAA AGGTCTTAAG AAGCATTTAT GAATTTCTTA GTACAGAGAA AAGGGAATTC 
CGCTTTCAAC TTCGAGGAGT TTCTTTTGTA ATGGTGGAAG AAGGTTGGAA GCTTCTGAAG 
CCTGAAGAGG TGGTAATAAA CCTTGAGTAT GAATCTGATT TTAAACCTTA TCTTTACAAA 
CTTCCTTTAG AACTTGGTAC TTTCCATCAG TTATTTAAAC ATTTGGGTAC TGAAGATATT 
ATTTCAACAA AGCAGTATGT TGAAGTTCTA GGTCGCATAT ACAAGAGCTC GGAAGGAAAG 
CAATTGGATC CTAATGAAAT GCGCACGGTT AAAAGAGTAG TTTCTGGCCT GTTCAAAAGT 
CTTCAGAATG ATTCTGTCAA GGTTAGGAAT GACCTTGAGA ATATGAGGGA TTTTGCCCTT 
TACCTTCCGA GTCAAGATGG TAGGTTGGTA AAGTCTAGTA TCCTAGTTTT TGATGATGCA 
CCTCACTACA AAAGCAGAAT CCAAGGTAAC ATTGGTGTGC AAATGCTTGT TGATCTTAGC 
CAGTGTTACT TAGGTAAAGA CCATGGCTTT CACACTAAAT TGATTATGTT ATTCCCTCAG 
AAATTGAGGC CTCGCTTACT TAGCAGTATT CTCGAAGAAC AGTTGGATGA AGAGTCTCCC 
AAAATCTGTC AGTTTGGAGC ATTATGTTCT TTGCAGGGAA GGTTACAGTT ACTGTTGTCT 
TCGGAACAAT TCATCACAGG ACTCATTAGG ATTATGAAGC ATGAAAATGA CAATGCTTTT 
TTAGCTAATG AGGAAAAAGC AGTAAGACTT TGCAAAGCTT TGCGAGAAGG CTTGAAAGTT 
TCCTGTTTTG AGAAGTTGCA AACAACGTTA CGAGTTAAAG GCTTTGCTCC TATTCCCCAC 
AGCAAAAGCG AAACATTTGC TTTCCTGAAG AGATACGGAA ATGCAGTAAT ACTGCTTTAC 
ATACAGCATT CTGACAGCAA AGACATAAAT TTCCTGTTGG CATTAGCCAT GACCTTAAAA 
TCTGCAACTG ACAACCTGAT TTCTGATACC TCATATTTAA TTGCCATGCT GGGTTGTAAT 
GATATTTACC GGATCAGTGA AAAGCTTGAC AGTTTAGGAG TGAAGTACGA CTCTTCTGAG 
CCATCAAAAC TTGAACTACC AACACCTGGC ACTCCTATAC CAGCTGAAAT TCACTATACA 
CTTCTTATGG ATCCAATGAA TGTATTTTAC CCTGGTGAAT ATGTTGGTTA CCTGGTTGAT 
GCTGAAGGTG GTGATATATA CGGATCATAT CAACCAACTT ACACCTATGC AATCATTGTA 
CAAGAAGTGG AAAGAGAAGA TGATGAAAAT CCCAGTTTTC TAGGGAAGAT TTACCAGATC 
GATATTGGAT ACAGTGAGTA TAAAATAGTG AGCTCTCTTG ACTTGTACAA ATTTTCAAGA 
CCGGAGGAAA GTTCTCAAAG CAGGGATAGC ACACCTTCTA CCCCAACCAG TCCTACAGAA 
TTTCCAGCAC ACGGACCTAG GGCCATTCCA CCTCTCTTCG CTGGTAGAGA GAGCCACAAA 
ACAATGTCCT CAAAACATCA TTCTCCAAAA AAGATAAAGT CAAACTCATT GCCAGAGATA 
TTAAGAGAAG TTACATCTGT GATTGAACAG GCTTGGAAGC TTCCAGAATC TGAAAGAAAA 
AAGATTATTA GGAGGCTGTA TCTTAAATGG CATCCAGATA AAAATGCAGA GAATCTTGAT 
ATAGCTAATG AAGTTTTCAA ACATTTACAG AATGAGATTA ACAGGCTAGA GAAACAGGCC 
TTCATGGATC AAAATATGGA CAGAGCCTCG AGAAGACCAT TTTCATCCTC TGCTTCTCGA 
TTTCAGTCGG ATAAATTTTC ATTTCAAAGA TTTTATACTT CATGGAATCA AGAAGCAACA 
AGCCACAAAT CTGAAAGGCA GCAGTATAAA GAAAAGTGTC CATCTTCCAC GGCGCCATCT 
TACTCGCAGC GCTTTTTTGT CCCACCTACC TTCAGGTCCG TTGGCAATCC TGTAGAGGCA 
CGTCGGTGGC TTAGGCAGGC CCGAGCTAAC TTCTCAGCTG CAAGAAATGA CCTTCATAAA 
AATGCCAATG AATGGGTATG CTTTAAGTGC TACCTTTCTA CTAAACTCGC CTTGATTGCA 
GCTGACTATG CTGTGAAGGG CAAATCGGAC AAAGACGTAA AACCAGCAGC CCTTGCACAA 
AAAATAGAGG AATACAGTCA GCAACTTGAA GGGCTTACAA ATGATGTTCA GACGTTGGAA 
GCTTACGGAG TAGATAGCTT AAAAACTAGG TATCCTGACT TACTGCCTTT CCCACAGATT 
CCCAATGATA GATTTACTTC AGAAGTTGCT ATGAGAGTGA TGGAATGTAC TGCTTGTATC 
ATAATAAAAC TTGAGAACTT TATACAACAG AAGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SACS cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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