Nefl cDNA ORF clone, Castor canadensis(American beaver)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nefl
Entrez Gene ID	109677700
Full Name	neurofilament light
Gene Type	protein-coding
Organism	Castor canadensis(American beaver)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020153546.1	XP_020009135.1	neurofilament light polypeptide

Pathways from BioSystems
Gene Ontology

The following Nefl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nefl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa289882	XM_020153546.1 Latest version!	Castor canadensis neurofilament light (Nefl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nefl ( XM_020153546.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020009135.1 Castor canadensis neurofilament light (Nefl), mRNA.

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CloneID	OCa289882
Clone ID Related Accession (Same CDS sequence)	XM_020153546.1
Accession Version	XM_020153546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	neurofilament light polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017884268.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCT TCAGCTACGA TCCGTATTAC TCGACCTCCT ACAAGCGGCG CTACGTGGAG 
ACGCCCCGGG TTCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCACG CTCCGCTTAC 
TCCAGCTACT CGGTCCCCGT CTCCTCCTCG CTGTCCGTGC GCCGCAGCTA CTCTTCCAGC 
TCCGGTTCTT TGATGCCCAG CCTGGAGAAC CTCGACCTGA GTCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACGCAGGAG AAGGCGCAGC TCCAGGACCT CAACGATCGC 
TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTAGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCGTCCC GCTTCCGGGC GCTGTACGAG 
CAGGAGATCC GCGACTTGCG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAAGCGCTC 
CAGGGCGAGC GCGAAGGGCT GGAGGAGACT CTGCGCAACC TGCAGGCGCG CTATGAAGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGCGCCGAC 
GAGGCCGCGC TCGCCCGCGC CGAGCTCGAA AAGCGCATCG ACAGCCTGAT GGACGAAATC 
GCTTTCCTGA AGAAGGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCTCA GATCCAGTAC 
GCGCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCTG ACCTCTCCGC GGCGCTCAAG 
GACATCCGCG CGCAATATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC CGAAGAATGG 
TTCAAGAGCC GCTTCACCGT GCTGACCGAG AGTGCCGCCA AGAACACCGA TGCCGTGCGC 
GCTGCCAAGG ATGAGGTGTC GGAGAGCCGC CGCCTGCTCA AGGCCAAGAC CCTGGAGATT 
GAAGCATGCC GGGGCATGAA CGAAGCGCTG GAGAAGCAGC TGCAGGAGCT GGAGGACAAG 
CAGAACGCAG ACATCAGTGC TATGCAGGAC ACAATCAACA AATTAGAAAA TGAATTGAGA 
ACCACAAAGA GTGAAATGGC AAGGTACCTG AAAGAATATC AGGACCTCCT CAACGTGAAG 
ATGGCACTGG ACATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGTGA AGAGACCAGG 
CTGAGCTTCA CCAGCGTGGG CAGCATCACC AGTGGCTACT CCCAGAGCTC CCAGGTCTTT 
GGCCGATCTG CCTATGGTGG TCTGCAGACC AGCTCCTACT TGATGTCCGC CCGCTCCTTC 
CCCTCCTACT ACACCAGCCA TGTCCAGGAA GAGCAGATAG AGGTGGAGGA GACCATTGAG 
GCCGCCAAAG CCGAGGAAGC CAAGGATGAG CCTCCCTCTG AAGGGGAAGC TGAGGAAGAG 
GAGAAGGAGA AGGAAGAGGG TGAGGAAGAG GAGGGAGCCG AAGAGGAAGA AGCAGCTAAG 
GAGGAATCTG AAGAGGCAAA GGAGGAAGAG GAAGAGGAAG GTGAGGAAGG AGAAGAAACC 
AAAGAATCAG AAGAGGAGGA GAAGAAAGAT GAAGGTGCTG AGGAGGAAAA AGCAACTAAG 
AAGAAAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020009135.1
CDS	362..1993
Translation	MSSFSYDPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSVPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSARSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKEESEEAKEEEEEEGEEGEETKESEEEEKKDEGAEEEKATKKKD

Target ORF information:

RefSeq Version	XM_020153546.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis neurofilament light (Nefl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020153546.1

ORF Insert Sequence:

ATGAGTTCCT TCAGCTACGA TCCGTATTAC TCGACCTCCT ACAAGCGGCG CTACGTGGAG 
ACGCCCCGGG TTCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCACG CTCCGCTTAC 
TCCAGCTACT CGGTCCCCGT CTCCTCCTCG CTGTCCGTGC GCCGCAGCTA CTCTTCCAGC 
TCCGGTTCTT TGATGCCCAG CCTGGAGAAC CTCGACCTGA GTCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACGCAGGAG AAGGCGCAGC TCCAGGACCT CAACGATCGC 
TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTAGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCGTCCC GCTTCCGGGC GCTGTACGAG 
CAGGAGATCC GCGACTTGCG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAAGCGCTC 
CAGGGCGAGC GCGAAGGGCT GGAGGAGACT CTGCGCAACC TGCAGGCGCG CTATGAAGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGCGCCGAC 
GAGGCCGCGC TCGCCCGCGC CGAGCTCGAA AAGCGCATCG ACAGCCTGAT GGACGAAATC 
GCTTTCCTGA AGAAGGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCTCA GATCCAGTAC 
GCGCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCTG ACCTCTCCGC GGCGCTCAAG 
GACATCCGCG CGCAATATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC CGAAGAATGG 
TTCAAGAGCC GCTTCACCGT GCTGACCGAG AGTGCCGCCA AGAACACCGA TGCCGTGCGC 
GCTGCCAAGG ATGAGGTGTC GGAGAGCCGC CGCCTGCTCA AGGCCAAGAC CCTGGAGATT 
GAAGCATGCC GGGGCATGAA CGAAGCGCTG GAGAAGCAGC TGCAGGAGCT GGAGGACAAG 
CAGAACGCAG ACATCAGTGC TATGCAGGAC ACAATCAACA AATTAGAAAA TGAATTGAGA 
ACCACAAAGA GTGAAATGGC AAGGTACCTG AAAGAATATC AGGACCTCCT CAACGTGAAG 
ATGGCACTGG ACATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGTGA AGAGACCAGG 
CTGAGCTTCA CCAGCGTGGG CAGCATCACC AGTGGCTACT CCCAGAGCTC CCAGGTCTTT 
GGCCGATCTG CCTATGGTGG TCTGCAGACC AGCTCCTACT TGATGTCCGC CCGCTCCTTC 
CCCTCCTACT ACACCAGCCA TGTCCAGGAA GAGCAGATAG AGGTGGAGGA GACCATTGAG 
GCCGCCAAAG CCGAGGAAGC CAAGGATGAG CCTCCCTCTG AAGGGGAAGC TGAGGAAGAG 
GAGAAGGAGA AGGAAGAGGG TGAGGAAGAG GAGGGAGCCG AAGAGGAAGA AGCAGCTAAG 
GAGGAATCTG AAGAGGCAAA GGAGGAAGAG GAAGAGGAAG GTGAGGAAGG AGAAGAAACC 
AAAGAATCAG AAGAGGAGGA GAAGAAAGAT GAAGGTGCTG AGGAGGAAAA AGCAACTAAG 
AAGAAAGATT GA