Limk2 cDNA ORF clone, Castor canadensis(American beaver)

Gene
Clones
gRNAs

Summary

Gene Symbol	Limk2
Entrez Gene ID	109696918
Full Name	LIM domain kinase 2
Gene Type	protein-coding
Organism	Castor canadensis(American beaver)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020180217.1	XP_020035806.1	LOW QUALITY PROTEIN: LIM domain kinase 2

Pathways from BioSystems
Gene Ontology

The following Limk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Limk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa295786	XM_020180217.1 Latest version!	Castor canadensis LIM domain kinase 2 (Limk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Limk2 ( XM_020180217.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020035806.1 Castor canadensis LIM domain kinase 2 (Limk2), mRNA.

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CloneID	OCa295786
Clone ID Related Accession (Same CDS sequence)	XM_020180217.1
Accession Version	XM_020180217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	LOW QUALITY PROTEIN: LIM domain kinase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017871927.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCCGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGGGA TCATGTCACT 
CCAAGCCAGA GACTGTATAG GACTGTCAAC GTAGCCTGGC ACAGCTCCTG CTTCCGGTGT 
TCGGAATGCC AGGATTCCCT CACCAACTGG TACTATGAGA AAGATGGGAA ACTCTACTGC 
CACNAAGACT ACTGGGGGAA ATTTGGGGAG TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGACCTGTCA TGGTGGCTGG GGAGTTTAAA TACCACCCAG AGTGCTTTGC CTGTATGAGC 
TGCAAGGTGA TCATTGAGGA CGGAGATGCC TACGCCCTGG TACAGCACGC TACTCTCTAC 
TGTGGGAAGT GCCACAATGA GGTGGTCCTG GCACCCATGT TTGAGAGGCT GTCCACAGAG 
TCTGTTCAGG ACCAGCTACC CTACTCTGTC ACCCTCATCT CCATGCCAGC CACCACCGAG 
GGCAGGCGAG GCTTCTCTGT GTCTGTGGAG AGTGCCTGCT CCAACTATGC CACCACTGTG 
CAAGTGAAAG AAGTAAACCG GATGCACATT AGTGCTAACA ACCGCAATGC CATCCATCCT 
GGGGACCGCA TTCTGGAGAT TAACGGGACC CCCGTCCGCA CACTCCGAGT GGAGGAGGTG 
GAGGAAGCGA TCAGCCAGAC GAGCCAGACG CTACAGCTGT TGATTGAACA TGACCCCGTC 
ACCCAACGCC TGGACCATCT GCGGCTAGGT GCCCAGCTTT CTGCCCACAT GCAGAATGCT 
GGGCAGTCCC ACACCCTCAG CCCTCTGGAC ACCAAGGAGA ATCTGGAAGG AACGCTAAGG 
AGACGCTCCC TGAGACGCAG TAACAGCATC TCCAGATCAC CTGGCCCCAG CTCCCCCAAG 
GAGCCCTTGC TGCTCAGCCG TGATATCAGC CGCTCTGAAT CCCTCCGCTG CCCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCATGTGAC CTGATCCACG GAGAGGTCCT GGGGAAGGGC 
TTCTTTGGGC AGGCCATCAA GGTGACACAC AAAGCCACGG GTAAAGTGAT GGTCATGAAG 
GAGTTAATTC GATGTGATGA GGAGACACAG AAGACTTTTC TGACTGAGGT GAAAGTGATG 
CGCAGCCTTG ACCACCCCAA TGTGCTCAAG TTCATTGGCG TCCTGTACAA GGACAAGAGG 
CTGAACCTGC TGACAGAGTA CATTGAAGGG GGCACGCTGA AGGACTTCCT ACGCAGCGTG 
GACCGGTTCC CCTGGCAACA GAAGGTCAGG TTCGCGAAAG GCATCGCCTC TGGAATGGCC 
TATTTGCACT CCATGTGCAT CATCCATCGG GATCTGAACT CACACAACTG TCTCATCAAG 
CTGGACAAGA CTGTGGTGGT GGCAGACTTT GGACTGTCAC GTCTCATCGT AGAAGAGAGG 
AAAAGGTCCC CAGTGGAGAA GGCCAGCACG AAGAAACGCA CCTTGCGCAA GAATGACCGC 
AAGAAGCGTT ACACAGTGGT GGGGAACCCC TACTGGATGG CCCCTGAGAT GCTCAACGGA 
AAAAGCTACG ATGAGACAGT GGACGTCTTC TCCTTCGGGA TCGTTCTCTG TGAGATCATT 
GGGCAGGTGT ACGCGGATCC CGACTGCCTG CCCCGCACAC TGGACTTTGG CCTCAACGTG 
AAGCTTTTCT GGGAGAAGTT TGTCCCCACA GACTGTCCCC CAGCCTTCTT CCCCCTCGCT 
GCGATCTGCT GCAAACTGGA GCCTGAGAGC AGACCAACAT TCTCAAAACT GGAGGACTCC 
TTTGAGGCCC TCTCACTGTA CCTGGGAGAG CTGGGCATCC CGCTGCCTGC AGAGCTAGAG 
GAGCTGGACC ACACTGTAAG CGTGCAGTAC GGCCTGACGC GGGACTCGCC CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020035806.1
CDS	115..2031
Translation	MAALAGEEAWRCRGCGDHVTPSQRLYRTVNVAWHSSCFRCSECQDSLTNWYYEKDGKLYCHnullDYWGKFGEFCHGCSLLMTGPVMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISANNRNAIHPGDRILEINGTPVRTLRVEEVEEAISQTSQTLQLLIEHDPVTQRLDHLRLGAQLSAHMQNAGQSHTLSPLDTKENLEGTLRRRSLRRSNSISRSPGPSSPKEPLLLSRDISRSESLRCPSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKRLNLLTEYIEGGTLKDFLRSVDRFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRSPVEKASTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESRPTFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSVQYGLTRDSPP

Target ORF information:

RefSeq Version	XM_020180217.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis LIM domain kinase 2 (Limk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020180217.1

ORF Insert Sequence:

ATGGCCGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGGGA TCATGTCACT 
CCAAGCCAGA GACTGTATAG GACTGTCAAC GTAGCCTGGC ACAGCTCCTG CTTCCGGTGT 
TCGGAATGCC AGGATTCCCT CACCAACTGG TACTATGAGA AAGATGGGAA ACTCTACTGC 
CACNAAGACT ACTGGGGGAA ATTTGGGGAG TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGACCTGTCA TGGTGGCTGG GGAGTTTAAA TACCACCCAG AGTGCTTTGC CTGTATGAGC 
TGCAAGGTGA TCATTGAGGA CGGAGATGCC TACGCCCTGG TACAGCACGC TACTCTCTAC 
TGTGGGAAGT GCCACAATGA GGTGGTCCTG GCACCCATGT TTGAGAGGCT GTCCACAGAG 
TCTGTTCAGG ACCAGCTACC CTACTCTGTC ACCCTCATCT CCATGCCAGC CACCACCGAG 
GGCAGGCGAG GCTTCTCTGT GTCTGTGGAG AGTGCCTGCT CCAACTATGC CACCACTGTG 
CAAGTGAAAG AAGTAAACCG GATGCACATT AGTGCTAACA ACCGCAATGC CATCCATCCT 
GGGGACCGCA TTCTGGAGAT TAACGGGACC CCCGTCCGCA CACTCCGAGT GGAGGAGGTG 
GAGGAAGCGA TCAGCCAGAC GAGCCAGACG CTACAGCTGT TGATTGAACA TGACCCCGTC 
ACCCAACGCC TGGACCATCT GCGGCTAGGT GCCCAGCTTT CTGCCCACAT GCAGAATGCT 
GGGCAGTCCC ACACCCTCAG CCCTCTGGAC ACCAAGGAGA ATCTGGAAGG AACGCTAAGG 
AGACGCTCCC TGAGACGCAG TAACAGCATC TCCAGATCAC CTGGCCCCAG CTCCCCCAAG 
GAGCCCTTGC TGCTCAGCCG TGATATCAGC CGCTCTGAAT CCCTCCGCTG CCCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCATGTGAC CTGATCCACG GAGAGGTCCT GGGGAAGGGC 
TTCTTTGGGC AGGCCATCAA GGTGACACAC AAAGCCACGG GTAAAGTGAT GGTCATGAAG 
GAGTTAATTC GATGTGATGA GGAGACACAG AAGACTTTTC TGACTGAGGT GAAAGTGATG 
CGCAGCCTTG ACCACCCCAA TGTGCTCAAG TTCATTGGCG TCCTGTACAA GGACAAGAGG 
CTGAACCTGC TGACAGAGTA CATTGAAGGG GGCACGCTGA AGGACTTCCT ACGCAGCGTG 
GACCGGTTCC CCTGGCAACA GAAGGTCAGG TTCGCGAAAG GCATCGCCTC TGGAATGGCC 
TATTTGCACT CCATGTGCAT CATCCATCGG GATCTGAACT CACACAACTG TCTCATCAAG 
CTGGACAAGA CTGTGGTGGT GGCAGACTTT GGACTGTCAC GTCTCATCGT AGAAGAGAGG 
AAAAGGTCCC CAGTGGAGAA GGCCAGCACG AAGAAACGCA CCTTGCGCAA GAATGACCGC 
AAGAAGCGTT ACACAGTGGT GGGGAACCCC TACTGGATGG CCCCTGAGAT GCTCAACGGA 
AAAAGCTACG ATGAGACAGT GGACGTCTTC TCCTTCGGGA TCGTTCTCTG TGAGATCATT 
GGGCAGGTGT ACGCGGATCC CGACTGCCTG CCCCGCACAC TGGACTTTGG CCTCAACGTG 
AAGCTTTTCT GGGAGAAGTT TGTCCCCACA GACTGTCCCC CAGCCTTCTT CCCCCTCGCT 
GCGATCTGCT GCAAACTGGA GCCTGAGAGC AGACCAACAT TCTCAAAACT GGAGGACTCC 
TTTGAGGCCC TCTCACTGTA CCTGGGAGAG CTGGGCATCC CGCTGCCTGC AGAGCTAGAG 
GAGCTGGACC ACACTGTAAG CGTGCAGTAC GGCCTGACGC GGGACTCGCC CCCCTAG