CNAG_07513 cDNA ORF clone, Cryptococcus neoformans var. grubii H99

Gene
Clones
gRNAs

Summary

Gene Symbol	CNAG_07513
Entrez Gene ID	23890351
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Cryptococcus neoformans var. grubii H99

mRNA and Protein(s)

mRNA	Protein	Name
XM_012192461.1	XP_012047851.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation.
Janbon G, Ormerod KL, Paulet D, Byrnes EJ 3rd, Yadav V, Chatterjee G, Mullapudi N, Hon CC, Billmyre RB, Brunel F, Bahn YS, Chen W, Chen Y, Chow EW, Copp?e JY, Floyd-Averette A, Gaillardin C, Gerik KJ, Goldberg J, Gonzalez-Hilarion S, Gujja S, Hamlin JL, Hsueh YP, Ianiri G, Jones S, Kodira CD, Kozubowski L, Lam W, Marra M, Mesner LD, Mieczkowski PA, Moyrand F, Nielsen K, Proux C, Rossignol T, Schein JE, Sun S, Wollschlaeger C, Wood IA, Zeng Q, Neuv?glise C, Newlon CS, Perfect JR, Lodge JK, Idnurm A, Stajich JE, Kronstad JW, Sanyal K, Heitman J, Fraser JA, Cuomo CA, Dietrich FS
PLoS genetics10(4)e1004261(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CNAG_07513 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNAG_07513 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr135012	XM_012192461.1 Latest version!	Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_07513), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNAG_07513 ( XM_012192461.1 ) cDNA ORF clone, Cryptococcus neoformans var. grubii H99 -> XP_012047851.1 Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_07513), mRNA.

Price & Availability↑

CloneID	OCr135012
Clone ID Related Accession (Same CDS sequence)	XM_012192461.1
Accession Version	XM_012192461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Cryptococcus neoformans var. grubii H99
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026747).

ATGTTTCTAC ATGACCTTCT GGAACAATGC TTTGAGTTCA TTGCTATGGA CGGTGCAATC 
GGATGCCAAC CAACGGCCCT ACAGAAACAT TTATCCAACT TAAACTCCTC GTTTGACAAG 
TCTACTTTTA ACTATGTTTG GAGATCACTT TGTCTTCAAC CTACTATCGA AATCATCCTC 
ACTTCGGACC TTTTCACCAC ATCCACTGGT CACGTGGCTA CAGTTAAAGG AAAGAAAAGG 
AAGTACGGCA ATCAGGTCGA TTCACACGAT TTAATCAGAA TTCTCTGTGA GGACAATGGG 
GCTCAAAGAA AGGGAGAGGA AGTCTCAATC AAAGATTACG AGGCTTTAAG TGCAAAATGG 
GGAGATAGGC TTCGCATTAG GTGCACCGAA GACGAGATCT ACTTTCGTCT GACCGGCCAG 
CACGAAAGGC TCTCCAGCTT AACGGCTGAC AATTTCCAAG TCCTCCAGCT GATAGCTATG 
AGTCGTGAGA ACGGGATTGC ACTTATCGAT CTCGACTCTT CGTCTGGTTT GGAGAAAGTG 
GAGAACATTC ATGCTCGAGT AGATGTGCTC ACCGATCTTG GTCTATGCGT GAGACTGTCT 
CAGACTATTC GGAACAGCAT TGGCTCTATT CTTGTGCATT ACAAGTTCCT CCATCTTAAT 
TCTGCCTACC TCTCTCTCTG TCGATTGAAT CCTGAACTGC AAGACACTCT TCCACTCGAT 
GTATCACTCT CCCATATGAC ACGGAATGAG GACAATGACG ACTTTGTAAA CGAAGCCGAC 
GAAGAAGAAG ATCAATCGGT AAATAAACTT CGTCTAGACT TCTCCCCTTT GACAGAAACG 
GAAGTGTCAT CGGGACATAT AATCAGAGAG AGATTGCTGA AAACTCTCGA TCATCCCGAA 
CTCAAAAACC ATCTGCTAAG ACAAAGAGGC CTCTTATATG CACTAGGATG GGTAGGGCCT 
GTGAGCACCA AGCAGCGCAG GGCAGTAGAG CGCATCATTC GGGCTCTCGT TGACAGTGGA 
GTGATTGAAC GTGTTGCCGC TGGAGACAAG AAAATTCTGT GCATTCGCCT AACCAAATAT 
AGGACAGAAC TCGATAAAGA TGTGTTGGAG ATTAACGACA ATCCAAAGAA GGAGGTGCAG 
GAGGATCGGG ATTGTCTGCA AGTGATCGCT CACCGGGCGC CCTATCAATC AGCAAGCGGT 
ATATCAATGA GTCAAGCATT GGATTATCAA GTGTTGGATC TGATCGTGCA AGCTGGTACA 
GACGGGATTA TTAAAAATGA TCTTTGGCGG AAACTAAACT TCATGGTATC GAGATCGTTT 
GACCAAGTCA TCGAGCGCGT CACGGTTACC ACTACCCCGA ATCATCTATG GTCTCGAATA 
ATCATTAGGA CTAGAGAGAA CGAGAGCCGT GAACAACGAC ATCGTTACTT TTCAATATCG 
AGTTTTCTCG ATGTTGCACG GCAGCTCGGT CGAGACAGCG ATCTCGGTGA TGCCGCTAAA 
GAGCCGGGAA ACGTAGGACA GTTTGCCGAT ATCTCCTGCA GATCTCGCTA TCATGGAGCC 
CAAGAGTATC TTCAAGCTCT TGGGGGGACC GTCACGAAAA AGGCGATTAA AAGTCAGAAG 
AAGGATCCCA AGGATTTCAA GTATCAGGAT TCAACGCAAA AACGTGGACG ACCATCGACC 
ACTATTATTG TTTACGACGC TCATAACGGT GGAAGGAACC GTGGAATTAT CGGGGCTATC 
CCGGTCCAAC CAGATCTTCC GGCTCGTTTA ATTTATGACT CCGACTCCGG CCTTGTGATG 
ACTCCATCTC CTTTGTGGAG TGGAAAGGGA GCTCATCCTC CGTTTACAGA GGAACAGCGT 
CGTGAAGGAA AGGAGCCCGC GTGGTTTGAC AGGTATCCTC ACGCAAAAGA ACGTGCAACA 
CCGAGAAATT CTAAGAAAGT GAAGGGAAAG GGGCGGTCAG AAGTTGGAGC GGCTACAAAC 
GCTGGCAGCG TGGACACTCA TGAATCTAGA CAAATCAAAC GAATAAAAAG CGACGTCGAC 
CGCAAAGAAG ATATGGGCAA GCAGTTGTCG ACCGCCTCAC CTGGACCGTC AGCAGTGACT 
AGCGATCTAC ATGGTGATTC CATTCGCCAA GGCATATCGG CTGACGAAAC TTCCATTAAC 
ACTTCCTCCT CTTCCCTTCT TAATGCCAAG GGTCAGGGAG GGAAAGTGCC CAAGGACAAT 
AGATCCGGCA TTGTTTCCGC TCCTTCTGAG GTAAATCATA TTGGTTCACC TGTCACTGGA 
CCAGCCACGA AAACACAGAC ATTAAACACA CCTGTTGCAA GCAGTGGCAT GGTAGACGAT 
GCTGAACAGC GCGTCCCGGT AGTTGAGACC TCTGGACTTT CAGAGGCCTC AAAAGAGGTA 
GCCAACACCC TTGTTGACCT GTCGAATCTC CCACAGCTAA GTGAATCATT GCCTGTATCA 
TTGCCGGCTT CTCCTTCTGT TCTATCTGTG CACATTACAC ACGAAAGATC ATCGGGACCT 
CAGGGATGCT CAAATGGTGA ACCTCGTCTC ACAAAGTCTT TTATTTCATC TGCCAGTCCC 
GTTGAGGCCG AATCCGTTAC AGCGTCTTCC ACCCAAGCCC AAGCTAAGAT CATTGAAGGT 
GATGCCAAAC GAAAGGGCTC TTTAATGCCG GCGTCTGAAC AACCCTCATT AAAATCTGCT 
CGGTTGGTTA AGGGAGAGGA AATGACCAAC GTATCTGCAG GTGAAATGAG GTACACTCCG 
GATCAACGGG CGGTCCCATC ACTTTCTCCA GCTTCTTTGA GTGATGAGGC TGCAATGCCA 
ACTTTATCTT CTGATGACGG CGAGGGCAGG GAGGCAACGC CTTTGAAAGA GATGCCTCCA 
TCCACTTCAT TAACTCCTCT GCCAGCAAGA TTGAAAAAAG GAAAAAGTAT TGGTTCAACA 
AGCAGGAAAT CATCCACTCC TCGAGGTCTG TTAGACTTAC GCGAATCTTT ACAAGCGGAA 
GAGCTCCTAC AATTCATCCA TGATTTTGGC GGGATCATAG CACAAGCTAA GATCCAGAAT 
GAACATCACG CATGGACTGT CAAGCATGCT GGTTCTTCCC ATCCTCATTC CCCCAGCAGG 
GCATACACAA TGGATCGCAC CACCATCAAG CGGGCTCTTG GAGCTCTTGA CCGTCAAGGC 
CGCCTCAGAC ATACTACGAC TCAGGTTGCG ACCACTTTAG GAACATTCTC GTCTGTCAAA 
TTCTATTACG TGCCGGATCT TCCCTCTCAA CAGCTTAACA GCCATATTAA TAGGCATAGG 
CATGAGACCA CACAAACGGG AAAGCTGTTT AGGTCCTCCG TTGCTACGGA GCTTCCCCAA 
ATCGAGTTTT CAGACTTCCA TCGTTCAGAC CCCAGTGGTG GCAAAGTTTC TGTTGCCCCG 
CTTGAAGAGT CGGCTGTCAA ATCCAATTCG GAAAGGAGGG ATCTCCTGCT TACTGAGAGA 
GATGTAGTCA GTCAACTCTA TGGGTACAAA TTTGGCATGT TTGTTAGAGT CCGAACTCTC 
CATTGCGCCA TCGTCAACGC TCTCAAGACA CCTGACTCCC CTTCCATCAT CTCCACTTCC 
CCTCGAATCT TCTCACTTAC CTTACTTTTC GAGGAACTTC AGGCCAAAGA CTGGTACGCT 
ATCATGCAGT ATACTAAGCT CATGGAAGAT ATCGTGGCCT GGCTTGGGAC CCCCAGCAAT 
GGAGAGACCA AGCTCAAGGA TGTGCCACCC GAGTTTAGAC CTCCTAAGGG TTTTGGCGGA 
TCGCTTCCAA GGGACAGAAT TGGAAGATTG TTGGATGTGT TGGCGACTCT CAAGATCCTC 
ACACCGCTTG TAGGTGTGCA TGACGGAAAC GGCGACATCA AAATTGGTGA ACAAATGTCA 
TTCCGAGTAG CAGACTCAAG TGTTGCCCCC TACTATGTGC TACATGATTA TGCTCCGGTG 
TATCATGTTG CATTAGGATC TAGAGCCCCT CTCTTAGGCC TGCTTCCGGT CATGTTTGAA 
GGAGACATCA TAGCGTTCTG GGGAAAGTAC AGAGAGGCGG TTCGGGATAA CACACCTATC 
GATCTGCCTG ATAAAATCGA CCAGGCTTCT AACAGTCGCG ACCTAATGTT CCCCAGTATA 
GCCCACCTCA AAGATCCTAT TGAACTTGAC GCATCTTTCC TTCGCAGAAT GCAAACCCGG 
GCTAGGTGGG ATGACAAACC TCGATTGACA ACATTACAGC AGGATGCAAT CAGAGATGCG 
ATAGATTGGT CACGGGCAAC CATTCACATT ACTTCCCCAG AAGCACTTGA AAACTTCGCC 
TACGAAAACG CTCTTCCGAT TTCGATCATC CAGGCTGAGC TTCAGTCTGG CCAAAAACGC 
GCTCGAGAAA ACATAGCTCA TCGTGAGGAG AGGCTTCGCG ACAGCGCACG TCGAGCCCAG 
GAACGGCAAG AGAGACGGTT GCGCTCATAT CAAGAGCGAA TCGCAGGAGA AGAAGCAGCG 
TCACGTCAAG CCTGGGAAGA AAGAGTTGCA ACAAGTGCAG TGCGAAAGGG TGTGCCTTAT 
GATTCGGCGT TACTGGACTT CGTCAGTTCG AAAGCTCCAG CCAACATGAA GAAGGCGAAT 
GTTACAGATG GACTAGTGGA TTATTGGGTA GGTGTATGGG AACTGTGCAA GGAGATGGAT 
GGTGAGGAGA GGGGGCAACT TTTGGCACAA GGAAGAGTGT CTTTAAAGCA AAAAGACAAG 
GGGGCAGACA AAAAATTAAA GAGGAGAGCG AAGGGCAAGA AAGCTACAGG AACGCCTGGA 
CAAGGCGAGG CGCCAGACGT AGAGGGAACG ACGACTAAAA GAAAAAATCG TTCGAAACGC 
AAGTGGACGA CTGAAGACGA CGAACTTATG CTTGATAGCG AAGCCATCAT ACGGGCTCGT 
TCTCGAACCA ATGCTTATAA GGGCCGCGCT GCCATGTCCC AGCTTTACCC AAGAATCACA 
GCAAGCACTT TCCGTATGCG TATTATGAAA ATTGCTGGCG AGCCCGGCAA GCTGGCATAC 
CTAGAACGGT TGGAACAGGC GTGGTACGAA CTTTGGCTCA AATATAGAGG GACTGAAGAA 
TTGCCGGATG ATAATATCGA GAGTTCCGTG GAGTTTGACT TGAAAGCTCA CATCGACTTC 
CTGAGAAAAC ACGTCGACAA GCGTACATTG AAATTGCTCG CCGCTTCTAC GCCCATTACA 
TCAGGTTTCC ATGCCCCAGA TCTCCCCTGC GACTTTTTTG ATCTCCACAA CCGATTTGAT 
TGGGAATATT CCAAGACCAG TAGACACACA TTTGATACTG TTGCTGAATC TCTAAGCGCC 
GAAGAGATAC GACTCAATAA TTTGGCCCGA AAGTCACTGA TACATAGCCC CACCAGGGGG 
CAGACTGAGA CGGGGTCGAA TCTCAATAGG GAGATGGGCA AGCTTCGCGC AGTAATGAAA 
CTTATCGTCG CTACTCCTGC AGCCACATAT GATATCGATT GCGGCGAACG TTTACTGTCC 
TCATGGGACA CATCTATCAG AGAAATGGCT ACTGCCGAAT TAGTTGATGC GGGAGTTTTC 
AGGAAGCACC TTGTGGGAGC AACAGCGGGT ACTGCCGGAA GGTTTTATGA CTTCACGGCT 
CAATGGCAGC AGTTGTCGGA TGGCCCACTG CCAACCACGT TCTGGGAAGA AGCAGAGGTG 
CTGGAGACGA AATTTAAAGA CGCTGGCGAC GAAGGATTCG AGTGGCCGTT GATCGGGCAA 
TCGGGTGAAC TGGGTAAATT GATGAGTATG GTATCAAATG ATGAGGCCGA ATTTTCCTTT 
AACATTAAGG ACTTCCCGGC CATCAACATC CCCCATTATA ACACCAGAAA ACTTAATGAT 
GATGCTTATG AGTTCGACTT CAAAGTCAAA AGGTCAGTCC TCGCTTGTCC GCCACTTGCG 
GCCTCTGCAC CGAATCCATT CAAATGCAAT GTTCCTTCTC CGTGGAACAC CACTGCCCTT 
GACGTCGAAC AATCCCGGCA GGTCCTGGAA GCTGCCAGTC GAGTGATAGA TGCTGTCATT 
GCCGCTGGTC CCGAAGGTAT CACCAAATCA TCACTTCAAA GCATGCTCAA TCTTTCCATT 
AATTTACTCG AAGGAGTCTT TAATACTTTT CAGGAGGATT CCCCTGCGGT ATTTTGGTCC 
GGCTACGATA CCGCGCGTCT CGTGGCGGCG GAATTCTGGC CCTCATGGAC AATTAGGACA 
CGCCCTTTCG AGCTTAAAGA GAAAAAAGAG AGCGAAAGGT TTACAACCCC ACGCCGTTGG 
CTTGACGTTT ACGGTCAAGT GTTGCATACA GAGTGGATTC GTGCGGTTAA TCGCGTCGCG 
AGTCATTTGA TGATGAGGCC TGGTATTAGC CAGCGTCGGT TGCGAGGGAA GTTGGATTTA 
ATTTTGGACC GGCTTGAGTT AGTAGATGTT TTGGAGTATC TTGTGAAAAG GGGTATTGGA 
CAACGGAGAT GGATGGGGCC CGGAGGGATG AACATGCCTC CTGTGGAGGC TACAAGCATA 
GAAGAGGAGG AATGGGTGGT ATGGTCACTG GATACCAATT TGTCTTTTTC AACTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012047851.1
CDS	210..6809
Translation	MFLHDLLEQCFEFIAMDGAIGCQPTALQKHLSNLNSSFDKSTFNYVWRSLCLQPTIEIILTSDLFTTSTGHVATVKGKKRKYGNQVDSHDLIRILCEDNGAQRKGEEVSIKDYEALSAKWGDRLRIRCTEDEIYFRLTGQHERLSSLTADNFQVLQLIAMSRENGIALIDLDSSSGLEKVENIHARVDVLTDLGLCVRLSQTIRNSIGSILVHYKFLHLNSAYLSLCRLNPELQDTLPLDVSLSHMTRNEDNDDFVNEADEEEDQSVNKLRLDFSPLTETEVSSGHIIRERLLKTLDHPELKNHLLRQRGLLYALGWVGPVSTKQRRAVERIIRALVDSGVIERVAAGDKKILCIRLTKYRTELDKDVLEINDNPKKEVQEDRDCLQVIAHRAPYQSASGISMSQALDYQVLDLIVQAGTDGIIKNDLWRKLNFMVSRSFDQVIERVTVTTTPNHLWSRIIIRTRENESREQRHRYFSISSFLDVARQLGRDSDLGDAAKEPGNVGQFADISCRSRYHGAQEYLQALGGTVTKKAIKSQKKDPKDFKYQDSTQKRGRPSTTIIVYDAHNGGRNRGIIGAIPVQPDLPARLIYDSDSGLVMTPSPLWSGKGAHPPFTEEQRREGKEPAWFDRYPHAKERATPRNSKKVKGKGRSEVGAATNAGSVDTHESRQIKRIKSDVDRKEDMGKQLSTASPGPSAVTSDLHGDSIRQGISADETSINTSSSSLLNAKGQGGKVPKDNRSGIVSAPSEVNHIGSPVTGPATKTQTLNTPVASSGMVDDAEQRVPVVETSGLSEASKEVANTLVDLSNLPQLSESLPVSLPASPSVLSVHITHERSSGPQGCSNGEPRLTKSFISSASPVEAESVTASSTQAQAKIIEGDAKRKGSLMPASEQPSLKSARLVKGEEMTNVSAGEMRYTPDQRAVPSLSPASLSDEAAMPTLSSDDGEGREATPLKEMPPSTSLTPLPARLKKGKSIGSTSRKSSTPRGLLDLRESLQAEELLQFIHDFGGIIAQAKIQNEHHAWTVKHAGSSHPHSPSRAYTMDRTTIKRALGALDRQGRLRHTTTQVATTLGTFSSVKFYYVPDLPSQQLNSHINRHRHETTQTGKLFRSSVATELPQIEFSDFHRSDPSGGKVSVAPLEESAVKSNSERRDLLLTERDVVSQLYGYKFGMFVRVRTLHCAIVNALKTPDSPSIISTSPRIFSLTLLFEELQAKDWYAIMQYTKLMEDIVAWLGTPSNGETKLKDVPPEFRPPKGFGGSLPRDRIGRLLDVLATLKILTPLVGVHDGNGDIKIGEQMSFRVADSSVAPYYVLHDYAPVYHVALGSRAPLLGLLPVMFEGDIIAFWGKYREAVRDNTPIDLPDKIDQASNSRDLMFPSIAHLKDPIELDASFLRRMQTRARWDDKPRLTTLQQDAIRDAIDWSRATIHITSPEALENFAYENALPISIIQAELQSGQKRARENIAHREERLRDSARRAQERQERRLRSYQERIAGEEAASRQAWEERVATSAVRKGVPYDSALLDFVSSKAPANMKKANVTDGLVDYWVGVWELCKEMDGEERGQLLAQGRVSLKQKDKGADKKLKRRAKGKKATGTPGQGEAPDVEGTTTKRKNRSKRKWTTEDDELMLDSEAIIRARSRTNAYKGRAAMSQLYPRITASTFRMRIMKIAGEPGKLAYLERLEQAWYELWLKYRGTEELPDDNIESSVEFDLKAHIDFLRKHVDKRTLKLLAASTPITSGFHAPDLPCDFFDLHNRFDWEYSKTSRHTFDTVAESLSAEEIRLNNLARKSLIHSPTRGQTETGSNLNREMGKLRAVMKLIVATPAATYDIDCGERLLSSWDTSIREMATAELVDAGVFRKHLVGATAGTAGRFYDFTAQWQQLSDGPLPTTFWEEAEVLETKFKDAGDEGFEWPLIGQSGELGKLMSMVSNDEAEFSFNIKDFPAINIPHYNTRKLNDDAYEFDFKVKRSVLACPPLAASAPNPFKCNVPSPWNTTALDVEQSRQVLEAASRVIDAVIAAGPEGITKSSLQSMLNLSINLLEGVFNTFQEDSPAVFWSGYDTARLVAAEFWPSWTIRTRPFELKEKKESERFTTPRRWLDVYGQVLHTEWIRAVNRVASHLMMRPGISQRRLRGKLDLILDRLELVDVLEYLVKRGIGQRRWMGPGGMNMPPVEATSIEEEEWVVWSLDTNLSFSTA

Target ORF information:

RefSeq Version	XM_012192461.1
Organism	Cryptococcus neoformans var. grubii H99
Definition	Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_07513), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012192461.1

ORF Insert Sequence:

ATGTTTCTAC ATGACCTTCT GGAACAATGC TTTGAGTTCA TTGCTATGGA CGGTGCAATC 
GGATGCCAAC CAACGGCCCT ACAGAAACAT TTATCCAACT TAAACTCCTC GTTTGACAAG 
TCTACTTTTA ACTATGTTTG GAGATCACTT TGTCTTCAAC CTACTATCGA AATCATCCTC 
ACTTCGGACC TTTTCACCAC ATCCACTGGT CACGTGGCTA CAGTTAAAGG AAAGAAAAGG 
AAGTACGGCA ATCAGGTCGA TTCACACGAT TTAATCAGAA TTCTCTGTGA GGACAATGGG 
GCTCAAAGAA AGGGAGAGGA AGTCTCAATC AAAGATTACG AGGCTTTAAG TGCAAAATGG 
GGAGATAGGC TTCGCATTAG GTGCACCGAA GACGAGATCT ACTTTCGTCT GACCGGCCAG 
CACGAAAGGC TCTCCAGCTT AACGGCTGAC AATTTCCAAG TCCTCCAGCT GATAGCTATG 
AGTCGTGAGA ACGGGATTGC ACTTATCGAT CTCGACTCTT CGTCTGGTTT GGAGAAAGTG 
GAGAACATTC ATGCTCGAGT AGATGTGCTC ACCGATCTTG GTCTATGCGT GAGACTGTCT 
CAGACTATTC GGAACAGCAT TGGCTCTATT CTTGTGCATT ACAAGTTCCT CCATCTTAAT 
TCTGCCTACC TCTCTCTCTG TCGATTGAAT CCTGAACTGC AAGACACTCT TCCACTCGAT 
GTATCACTCT CCCATATGAC ACGGAATGAG GACAATGACG ACTTTGTAAA CGAAGCCGAC 
GAAGAAGAAG ATCAATCGGT AAATAAACTT CGTCTAGACT TCTCCCCTTT GACAGAAACG 
GAAGTGTCAT CGGGACATAT AATCAGAGAG AGATTGCTGA AAACTCTCGA TCATCCCGAA 
CTCAAAAACC ATCTGCTAAG ACAAAGAGGC CTCTTATATG CACTAGGATG GGTAGGGCCT 
GTGAGCACCA AGCAGCGCAG GGCAGTAGAG CGCATCATTC GGGCTCTCGT TGACAGTGGA 
GTGATTGAAC GTGTTGCCGC TGGAGACAAG AAAATTCTGT GCATTCGCCT AACCAAATAT 
AGGACAGAAC TCGATAAAGA TGTGTTGGAG ATTAACGACA ATCCAAAGAA GGAGGTGCAG 
GAGGATCGGG ATTGTCTGCA AGTGATCGCT CACCGGGCGC CCTATCAATC AGCAAGCGGT 
ATATCAATGA GTCAAGCATT GGATTATCAA GTGTTGGATC TGATCGTGCA AGCTGGTACA 
GACGGGATTA TTAAAAATGA TCTTTGGCGG AAACTAAACT TCATGGTATC GAGATCGTTT 
GACCAAGTCA TCGAGCGCGT CACGGTTACC ACTACCCCGA ATCATCTATG GTCTCGAATA 
ATCATTAGGA CTAGAGAGAA CGAGAGCCGT GAACAACGAC ATCGTTACTT TTCAATATCG 
AGTTTTCTCG ATGTTGCACG GCAGCTCGGT CGAGACAGCG ATCTCGGTGA TGCCGCTAAA 
GAGCCGGGAA ACGTAGGACA GTTTGCCGAT ATCTCCTGCA GATCTCGCTA TCATGGAGCC 
CAAGAGTATC TTCAAGCTCT TGGGGGGACC GTCACGAAAA AGGCGATTAA AAGTCAGAAG 
AAGGATCCCA AGGATTTCAA GTATCAGGAT TCAACGCAAA AACGTGGACG ACCATCGACC 
ACTATTATTG TTTACGACGC TCATAACGGT GGAAGGAACC GTGGAATTAT CGGGGCTATC 
CCGGTCCAAC CAGATCTTCC GGCTCGTTTA ATTTATGACT CCGACTCCGG CCTTGTGATG 
ACTCCATCTC CTTTGTGGAG TGGAAAGGGA GCTCATCCTC CGTTTACAGA GGAACAGCGT 
CGTGAAGGAA AGGAGCCCGC GTGGTTTGAC AGGTATCCTC ACGCAAAAGA ACGTGCAACA 
CCGAGAAATT CTAAGAAAGT GAAGGGAAAG GGGCGGTCAG AAGTTGGAGC GGCTACAAAC 
GCTGGCAGCG TGGACACTCA TGAATCTAGA CAAATCAAAC GAATAAAAAG CGACGTCGAC 
CGCAAAGAAG ATATGGGCAA GCAGTTGTCG ACCGCCTCAC CTGGACCGTC AGCAGTGACT 
AGCGATCTAC ATGGTGATTC CATTCGCCAA GGCATATCGG CTGACGAAAC TTCCATTAAC 
ACTTCCTCCT CTTCCCTTCT TAATGCCAAG GGTCAGGGAG GGAAAGTGCC CAAGGACAAT 
AGATCCGGCA TTGTTTCCGC TCCTTCTGAG GTAAATCATA TTGGTTCACC TGTCACTGGA 
CCAGCCACGA AAACACAGAC ATTAAACACA CCTGTTGCAA GCAGTGGCAT GGTAGACGAT 
GCTGAACAGC GCGTCCCGGT AGTTGAGACC TCTGGACTTT CAGAGGCCTC AAAAGAGGTA 
GCCAACACCC TTGTTGACCT GTCGAATCTC CCACAGCTAA GTGAATCATT GCCTGTATCA 
TTGCCGGCTT CTCCTTCTGT TCTATCTGTG CACATTACAC ACGAAAGATC ATCGGGACCT 
CAGGGATGCT CAAATGGTGA ACCTCGTCTC ACAAAGTCTT TTATTTCATC TGCCAGTCCC 
GTTGAGGCCG AATCCGTTAC AGCGTCTTCC ACCCAAGCCC AAGCTAAGAT CATTGAAGGT 
GATGCCAAAC GAAAGGGCTC TTTAATGCCG GCGTCTGAAC AACCCTCATT AAAATCTGCT 
CGGTTGGTTA AGGGAGAGGA AATGACCAAC GTATCTGCAG GTGAAATGAG GTACACTCCG 
GATCAACGGG CGGTCCCATC ACTTTCTCCA GCTTCTTTGA GTGATGAGGC TGCAATGCCA 
ACTTTATCTT CTGATGACGG CGAGGGCAGG GAGGCAACGC CTTTGAAAGA GATGCCTCCA 
TCCACTTCAT TAACTCCTCT GCCAGCAAGA TTGAAAAAAG GAAAAAGTAT TGGTTCAACA 
AGCAGGAAAT CATCCACTCC TCGAGGTCTG TTAGACTTAC GCGAATCTTT ACAAGCGGAA 
GAGCTCCTAC AATTCATCCA TGATTTTGGC GGGATCATAG CACAAGCTAA GATCCAGAAT 
GAACATCACG CATGGACTGT CAAGCATGCT GGTTCTTCCC ATCCTCATTC CCCCAGCAGG 
GCATACACAA TGGATCGCAC CACCATCAAG CGGGCTCTTG GAGCTCTTGA CCGTCAAGGC 
CGCCTCAGAC ATACTACGAC TCAGGTTGCG ACCACTTTAG GAACATTCTC GTCTGTCAAA 
TTCTATTACG TGCCGGATCT TCCCTCTCAA CAGCTTAACA GCCATATTAA TAGGCATAGG 
CATGAGACCA CACAAACGGG AAAGCTGTTT AGGTCCTCCG TTGCTACGGA GCTTCCCCAA 
ATCGAGTTTT CAGACTTCCA TCGTTCAGAC CCCAGTGGTG GCAAAGTTTC TGTTGCCCCG 
CTTGAAGAGT CGGCTGTCAA ATCCAATTCG GAAAGGAGGG ATCTCCTGCT TACTGAGAGA 
GATGTAGTCA GTCAACTCTA TGGGTACAAA TTTGGCATGT TTGTTAGAGT CCGAACTCTC 
CATTGCGCCA TCGTCAACGC TCTCAAGACA CCTGACTCCC CTTCCATCAT CTCCACTTCC 
CCTCGAATCT TCTCACTTAC CTTACTTTTC GAGGAACTTC AGGCCAAAGA CTGGTACGCT 
ATCATGCAGT ATACTAAGCT CATGGAAGAT ATCGTGGCCT GGCTTGGGAC CCCCAGCAAT 
GGAGAGACCA AGCTCAAGGA TGTGCCACCC GAGTTTAGAC CTCCTAAGGG TTTTGGCGGA 
TCGCTTCCAA GGGACAGAAT TGGAAGATTG TTGGATGTGT TGGCGACTCT CAAGATCCTC 
ACACCGCTTG TAGGTGTGCA TGACGGAAAC GGCGACATCA AAATTGGTGA ACAAATGTCA 
TTCCGAGTAG CAGACTCAAG TGTTGCCCCC TACTATGTGC TACATGATTA TGCTCCGGTG 
TATCATGTTG CATTAGGATC TAGAGCCCCT CTCTTAGGCC TGCTTCCGGT CATGTTTGAA 
GGAGACATCA TAGCGTTCTG GGGAAAGTAC AGAGAGGCGG TTCGGGATAA CACACCTATC 
GATCTGCCTG ATAAAATCGA CCAGGCTTCT AACAGTCGCG ACCTAATGTT CCCCAGTATA 
GCCCACCTCA AAGATCCTAT TGAACTTGAC GCATCTTTCC TTCGCAGAAT GCAAACCCGG 
GCTAGGTGGG ATGACAAACC TCGATTGACA ACATTACAGC AGGATGCAAT CAGAGATGCG 
ATAGATTGGT CACGGGCAAC CATTCACATT ACTTCCCCAG AAGCACTTGA AAACTTCGCC 
TACGAAAACG CTCTTCCGAT TTCGATCATC CAGGCTGAGC TTCAGTCTGG CCAAAAACGC 
GCTCGAGAAA ACATAGCTCA TCGTGAGGAG AGGCTTCGCG ACAGCGCACG TCGAGCCCAG 
GAACGGCAAG AGAGACGGTT GCGCTCATAT CAAGAGCGAA TCGCAGGAGA AGAAGCAGCG 
TCACGTCAAG CCTGGGAAGA AAGAGTTGCA ACAAGTGCAG TGCGAAAGGG TGTGCCTTAT 
GATTCGGCGT TACTGGACTT CGTCAGTTCG AAAGCTCCAG CCAACATGAA GAAGGCGAAT 
GTTACAGATG GACTAGTGGA TTATTGGGTA GGTGTATGGG AACTGTGCAA GGAGATGGAT 
GGTGAGGAGA GGGGGCAACT TTTGGCACAA GGAAGAGTGT CTTTAAAGCA AAAAGACAAG 
GGGGCAGACA AAAAATTAAA GAGGAGAGCG AAGGGCAAGA AAGCTACAGG AACGCCTGGA 
CAAGGCGAGG CGCCAGACGT AGAGGGAACG ACGACTAAAA GAAAAAATCG TTCGAAACGC 
AAGTGGACGA CTGAAGACGA CGAACTTATG CTTGATAGCG AAGCCATCAT ACGGGCTCGT 
TCTCGAACCA ATGCTTATAA GGGCCGCGCT GCCATGTCCC AGCTTTACCC AAGAATCACA 
GCAAGCACTT TCCGTATGCG TATTATGAAA ATTGCTGGCG AGCCCGGCAA GCTGGCATAC 
CTAGAACGGT TGGAACAGGC GTGGTACGAA CTTTGGCTCA AATATAGAGG GACTGAAGAA 
TTGCCGGATG ATAATATCGA GAGTTCCGTG GAGTTTGACT TGAAAGCTCA CATCGACTTC 
CTGAGAAAAC ACGTCGACAA GCGTACATTG AAATTGCTCG CCGCTTCTAC GCCCATTACA 
TCAGGTTTCC ATGCCCCAGA TCTCCCCTGC GACTTTTTTG ATCTCCACAA CCGATTTGAT 
TGGGAATATT CCAAGACCAG TAGACACACA TTTGATACTG TTGCTGAATC TCTAAGCGCC 
GAAGAGATAC GACTCAATAA TTTGGCCCGA AAGTCACTGA TACATAGCCC CACCAGGGGG 
CAGACTGAGA CGGGGTCGAA TCTCAATAGG GAGATGGGCA AGCTTCGCGC AGTAATGAAA 
CTTATCGTCG CTACTCCTGC AGCCACATAT GATATCGATT GCGGCGAACG TTTACTGTCC 
TCATGGGACA CATCTATCAG AGAAATGGCT ACTGCCGAAT TAGTTGATGC GGGAGTTTTC 
AGGAAGCACC TTGTGGGAGC AACAGCGGGT ACTGCCGGAA GGTTTTATGA CTTCACGGCT 
CAATGGCAGC AGTTGTCGGA TGGCCCACTG CCAACCACGT TCTGGGAAGA AGCAGAGGTG 
CTGGAGACGA AATTTAAAGA CGCTGGCGAC GAAGGATTCG AGTGGCCGTT GATCGGGCAA 
TCGGGTGAAC TGGGTAAATT GATGAGTATG GTATCAAATG ATGAGGCCGA ATTTTCCTTT 
AACATTAAGG ACTTCCCGGC CATCAACATC CCCCATTATA ACACCAGAAA ACTTAATGAT 
GATGCTTATG AGTTCGACTT CAAAGTCAAA AGGTCAGTCC TCGCTTGTCC GCCACTTGCG 
GCCTCTGCAC CGAATCCATT CAAATGCAAT GTTCCTTCTC CGTGGAACAC CACTGCCCTT 
GACGTCGAAC AATCCCGGCA GGTCCTGGAA GCTGCCAGTC GAGTGATAGA TGCTGTCATT 
GCCGCTGGTC CCGAAGGTAT CACCAAATCA TCACTTCAAA GCATGCTCAA TCTTTCCATT 
AATTTACTCG AAGGAGTCTT TAATACTTTT CAGGAGGATT CCCCTGCGGT ATTTTGGTCC 
GGCTACGATA CCGCGCGTCT CGTGGCGGCG GAATTCTGGC CCTCATGGAC AATTAGGACA 
CGCCCTTTCG AGCTTAAAGA GAAAAAAGAG AGCGAAAGGT TTACAACCCC ACGCCGTTGG 
CTTGACGTTT ACGGTCAAGT GTTGCATACA GAGTGGATTC GTGCGGTTAA TCGCGTCGCG 
AGTCATTTGA TGATGAGGCC TGGTATTAGC CAGCGTCGGT TGCGAGGGAA GTTGGATTTA 
ATTTTGGACC GGCTTGAGTT AGTAGATGTT TTGGAGTATC TTGTGAAAAG GGGTATTGGA 
CAACGGAGAT GGATGGGGCC CGGAGGGATG AACATGCCTC CTGTGGAGGC TACAAGCATA 
GAAGAGGAGG AATGGGTGGT ATGGTCACTG GATACCAATT TGTCTTTTTC AACTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation.
PLoS genetics10(4)e1004261(2014 Apr)
Janbon G,Ormerod KL,Paulet D,Byrnes EJ 3rd,Yadav V,Chatterjee G,Mullapudi N,Hon CC,Billmyre RB,Brunel F,Bahn YS,Chen W,Chen Y,Chow EW,Copp?e JY,Floyd-Averette A,Gaillardin C,Gerik KJ,Goldberg J,Gonzalez-Hilarion S,Gujja S,Hamlin JL,Hsueh YP,Ianiri G,Jones S,Kodira CD,Kozubowski L,Lam W,Marra M,Mesner LD,Mieczkowski PA,Moyrand F,Nielsen K,Proux C,Rossignol T,Schein JE,Sun S,Wollschlaeger C,Wood IA,Zeng Q,Neuv?glise C,Newlon CS,Perfect JR,Lodge JK,Idnurm A,Stajich JE,Kronstad JW,Sanyal K,Heitman J,Fraser JA,Cuomo CA,Dietrich FS

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CNAG_07513 cDNA ORF clone, Cryptococcus neoformans var. grubii H99

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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