nes cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nes
Entrez Gene ID	100150939
Full Name	nestin
General protein information	Preferred Names nestin Names nestin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001919887.6	XP_001919922.1	nestin
XM_001919887.7	XP_001919922.1	nestin

Pathways from BioSystems
Gene Ontology

The following nes gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nes cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa21583	XM_001919887.7 Latest version!	Danio rerio nestin (nes), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa21583	XM_001919887.6	Danio rerio nestin (nes), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nes ( XM_001919887.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001919922.1 Danio rerio nestin (nes), mRNA.

Price & Availability↑

CloneID	ODa21583
Clone ID Related Accession (Same CDS sequence)	XM_001919887.6 , XM_001919887.7
Accession Version	XM_001919887.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nestin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919887.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTC TGGGTGTCCG ACAGCCATTT GGCCCTCAGT TTCAGGAGGA AAAGTACCAG 
ATGCTAGAGC TGAACCACCG CCTTGAATCA TACTTGGGCC GTGTGAAACT TCTGGAGGAA 
GAGAACAAGC TACTTCGAGA GGAGATCCAC ACTCTCAAGA GCAGCAGGGA CCCAGCAGGC 
CAACGTAAAG CCCAGGAGGA GGCTCTCAGT CAGGCCAGGA GGATGATGGA GGAGGCCTGG 
AGAAAGAAAG ACTGTGTGGA GCTGGAGGTA GAGAATCTGA TGGAGGATAT GGAGAAGGTG 
AGCTTTCAAA GAAAGAAGGT GAAGACTGCA CAGGCTGAAG CTCAGAGGAA GCTCACAGAG 
AGCAGAAAGG AGCTGGAAGA GGAGCGCAGG GCTCAGATTT GGCTTAGAGA GAAGGTCGGT 
CAACTCGAGA AAGACCTCAT GCTGCAGATG CAAGTGCACC AGGAGAACAT GGAAACCATG 
CAGGCCTCAC TGAAACAGAC AAAGCAGGTC CTAATGGCTC CACAGCGTGT GCAAGCTACC 
AGCATCCCAG ACCTGGGGCA GGAGTATGGC TACAAGGCAG CTCAAGCCTG GCAGGAGGCA 
GCCAACAACT ATCAGAAACA GGTTGGACGT CTGGAGGAGA GTCTGAACCA GACAAAAGCT 
AACATGACAA AGATTTACCA GGAGAAGAGA GAGAGCCAGC GTCAGGTACA GCATCTGGCC 
AAAGAGCTGG AGAGCACGAG GATCAAGAGG CAGATGCTGG AGAAACATGC CATGCAGCAA 
AGAGAAGAAC AGAAAGAGGA GCTTCAACAT CTTCAGGCCC AAGTAAACAC CCTGGAGTTG 
GAGAAAGACT GTCTGGGGCA GCAGATTGAC AGCCTGATGT TGGATAGACA ACATCTGCTG 
CAGGTGAAGA TGTCTCTTGG ACTGGAGGTG GCAACATACA GAGCTTTGCT GGACAGCGAG 
GGCCTGAGAA TCGACAGACC AACACCAAAC AAGACCAGCT CTACTGTCTT TTTAGATGCA 
CTCTCAAAGC CCACTGGGAT CCAGTCAACT TCTCAAACCA CTGCTGCTTC CTGCCTTCTC 
AGCAGTTCTG TGTCTACAAG TCACAGATCA ATCGCCTCAT CCCGCAGTCT GCTGACCAGT 
GCGGCTCCGT CATGGACACT GACACGAGGG ACTCCACAGA GACCACCAAG CAGTACCTCG 
ATGACAGAAA AGACAGAGGA TCACATATCT GAGGAGACTA AGAGATCTGC TGAGGAGTCT 
GTGGATCAAT TACAGCAAGA GAAAGTCCAG CAAGACTGGA CTCTCGAATC CACTCTTCCC 
AAAACATCTG CAGAACCAAA ACCAGAGCTT CAACCAGAAG AAATTAAGGT GGAAGACGAA 
GCAAATGAAC CCCAACAAGC TCATATGGTT GAATCTAAAA CTGATGAATC TGAACTGACT 
AGTGTACCAG CTGAGCAACA AGGAAGTATG TCCCAAACTC CAGAGACAAA GAGCTGGGCT 
GGTCATTTCA ATGATCCTGC AGAAGTTTCA GAAGACGGAA AGGATGAAGA CACTGAAGTG 
TCTGTCGAAA TGGCTCGTAT CTCACATGCA CCCAAGGTTG CTTGGGAAGA AATCAAATCT 
GCAGTAGAGG ATGAAAAAGA TGATGCTTCT GAGATGGACG TAAGATCAGA GATCATCAGT 
GAAAGCCACA CATTTGCATA TGGAGATGCA GAGAATGATA GTAATACATT AACATCAAGC 
CATATCAGTC AAAACACCAA TGCACTGGCT TCATCTTTCC TTGAGCAAGG AACTCTAGAT 
TCAGCTAGTT ATTTTGGCTA TGAGGCAACA AATGAAAATC TTATGAAGGA AGAGGAACTG 
GACAATGTGA GCAATATTAG CGAGGAGGTG ACAGAACAAT TGAACAGTGA AACTGAGGCA 
GCTATTGATT CAATCAATGA GTGGGACAGA CAGGAAGAAA GTGAACCAGA GAATGAGACC 
AAAGTGATGA CTTCTTACTT TGAGGTAGAA GAGGGAGAAA TGAGCATCAA TACTGACACG 
AAAGATAAAA CTGAAGATGA CATAGAAAGG GAAGAGTTAG AAGTGACAGA AAGAGAAATT 
CTAGTGCAAA GTGCAGGTGG AATGCTGGGT GTAGATTTAC CCAATGACAC TGATCATCAG 
GATGAGCACA ATCTACCTCA GATAGAGCTG AACGAAGACC AATCTGAGGC AGAAGAAGAA 
AATCAAAAAG AAAAGCAATG TGATGAAGAT GACTCCCCAA ACATATCAGC CTCATTGAAA 
ACTGACCCAG GAGAAGGAGA CAGTTACAGC CAGGAGCACA CGCTGGCAGA CACACGGCCC 
TTGATCCGTT ATAAGAGCGA TGAGGAAACC TCACACCACA TCGGTGAGAC CAGTGACAGT 
GAGGATGAGA AAGAAAGAAT TGATCAAGGC CACTTGAATG AGAAACGTTT TAACACTATG 
GAGGATCTCA CTGAAGAACC AGACATGGAG GTCACCGGAA AGATGAGGCT GGAGGATCTT 
GTTTCTAATG AAGAAGCTGC AGCTGACGAT GTGGCATCTG TGATGTTTCA GAAAGTCTCT 
GGAGACCATG AAAGTTTGGA TGTGGTGGTG CAAGAAAGTG AACGCAAGGG TAATCTGGAG 
AAGGAAGATA GTGGTGATGC TGATAATATG AGAGACAATA AGGAAGAAGA TGTCAGTGTT 
TTTGAGCAAA ATGAGAACCA GCAACTGACT GAACACCAGC ATGTTCACAC AGAACAGGTT 
GAGGATAAAC CAGTCCATTC ACATGAAACT CAAGAACACG TCAACACGGA GTTTTCATCC 
ACATTTTCTG AAAGATCTCA GCAAGAGCAA CTGGAAGAAT CAAGTCTGAC TATGTTTGAA 
GATGAAGCTG CAACCAAAGA AGCAGAAGAT TCGGATGTCT CTATGCACAC CAACATAGAT 
CTGATTGACA GTCGCTCTTT AGAGAGCGAG ATAAACGGTC AGCCAGACAT TATGACCTCA 
GACATGGCCA ACTCTGAGTG CAATAGCTCA GAAGACGAGT CTCCAAATGC CAGCCAGTGC 
TTTCAGAACA CAAGCCTTTT AGCAGCGGCG ACTCCTAATG AACAGCCATT AACCTTTACT 
AATGAAGTCT CCAAGGCTGA TTATGTGTCT GACAACAACG ACGTGCCTGA AGAAGTGCTC 
AGTGAGGAGA AGAGCACTGA TGAGCCCAAG GCCTCTCAGA TTGATGACTT GGAGAATTTT 
AACATTTGGG GGGAAAGCAC GAGCATTTCA GATGCAGCCC AGACCACACA TGCAAGCATG 
GATGAACATT CAAGCATTTC CCCAGTGAAT GAGAATCTGG AAAGCTCAAA CAAAATACCA 
AGTGTGGCGG AAAACATCTT TGGACATTTT GAAGAACATC TCGAAAGGTC AGTGGAGAGC 
TTTCCAGAAA AAGAAGTGAC TGTGATGGAC TTTGAGAACA ACGATTTAGG AGAGGAATTT 
CAGTCGAAGC AAATGAAAAG TGAGATTCAC AGCTTCTTCA GCACTAGTTT GAAGCAAGAT 
TTCTGGAGCG AAGGGAAAAT GGAGATGGCA GCTACTTATG ATCCCGCCAA AACTGAAGAC 
TTAAATCAGG CCATGGTCTT TGGAGAGGAG TGGAGAGAAA TAGGAGTGCA ATCAGCGAAT 
GGAGATACTA AAGAAGAGAT GGAGATCCTC AAAATCAGGG ATGATAAGCA AAAAGATGGA 
CAGCCAGCGC AGAGCAAGAT AGTCCAGTCA GATGATTCTG CTGATGAGGG GGATTCCTGG 
TCATCTGGTG ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919922.1
CDS	142..3939
Translation	MELLGVRQPFGPQFQEEKYQMLELNHRLESYLGRVKLLEEENKLLREEIHTLKSSRDPAGQRKAQEEALSQARRMMEEAWRKKDCVELEVENLMEDMEKVSFQRKKVKTAQAEAQRKLTESRKELEEERRAQIWLREKVGQLEKDLMLQMQVHQENMETMQASLKQTKQVLMAPQRVQATSIPDLGQEYGYKAAQAWQEAANNYQKQVGRLEESLNQTKANMTKIYQEKRESQRQVQHLAKELESTRIKRQMLEKHAMQQREEQKEELQHLQAQVNTLELEKDCLGQQIDSLMLDRQHLLQVKMSLGLEVATYRALLDSEGLRIDRPTPNKTSSTVFLDALSKPTGIQSTSQTTAASCLLSSSVSTSHRSIASSRSLLTSAAPSWTLTRGTPQRPPSSTSMTEKTEDHISEETKRSAEESVDQLQQEKVQQDWTLESTLPKTSAEPKPELQPEEIKVEDEANEPQQAHMVESKTDESELTSVPAEQQGSMSQTPETKSWAGHFNDPAEVSEDGKDEDTEVSVEMARISHAPKVAWEEIKSAVEDEKDDASEMDVRSEIISESHTFAYGDAENDSNTLTSSHISQNTNALASSFLEQGTLDSASYFGYEATNENLMKEEELDNVSNISEEVTEQLNSETEAAIDSINEWDRQEESEPENETKVMTSYFEVEEGEMSINTDTKDKTEDDIEREELEVTEREILVQSAGGMLGVDLPNDTDHQDEHNLPQIELNEDQSEAEEENQKEKQCDEDDSPNISASLKTDPGEGDSYSQEHTLADTRPLIRYKSDEETSHHIGETSDSEDEKERIDQGHLNEKRFNTMEDLTEEPDMEVTGKMRLEDLVSNEEAAADDVASVMFQKVSGDHESLDVVVQESERKGNLEKEDSGDADNMRDNKEEDVSVFEQNENQQLTEHQHVHTEQVEDKPVHSHETQEHVNTEFSSTFSERSQQEQLEESSLTMFEDEAATKEAEDSDVSMHTNIDLIDSRSLESEINGQPDIMTSDMANSECNSSEDESPNASQCFQNTSLLAAATPNEQPLTFTNEVSKADYVSDNNDVPEEVLSEEKSTDEPKASQIDDLENFNIWGESTSISDAAQTTHASMDEHSSISPVNENLESSNKIPSVAENIFGHFEEHLERSVESFPEKEVTVMDFENNDLGEEFQSKQMKSEIHSFFSTSLKQDFWSEGKMEMAATYDPAKTEDLNQAMVFGEEWREIGVQSANGDTKEEMEILKIRDDKQKDGQPAQSKIVQSDDSADEGDSWSSGDE

Target ORF information:

RefSeq Version	XM_001919887.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nestin (nes), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919887.6

ORF Insert Sequence:

ATGGAGCTTC TGGGTGTCCG ACAGCCATTT GGCCCTCAGT TTCAGGAGGA AAAGTACCAG 
ATGCTAGAGC TGAACCACCG CCTTGAATCA TACTTGGGCC GTGTGAAACT TCTGGAGGAA 
GAGAACAAGC TACTTCGAGA GGAGATCCAC ACTCTCAAGA GCAGCAGGGA CCCAGCAGGC 
CAACGTAAAG CCCAGGAGGA GGCTCTCAGT CAGGCCAGGA GGATGATGGA GGAGGCCTGG 
AGAAAGAAAG ACTGTGTGGA GCTGGAGGTA GAGAATCTGA TGGAGGATAT GGAGAAGGTG 
AGCTTTCAAA GAAAGAAGGT GAAGACTGCA CAGGCTGAAG CTCAGAGGAA GCTCACAGAG 
AGCAGAAAGG AGCTGGAAGA GGAGCGCAGG GCTCAGATTT GGCTTAGAGA GAAGGTCGGT 
CAACTCGAGA AAGACCTCAT GCTGCAGATG CAAGTGCACC AGGAGAACAT GGAAACCATG 
CAGGCCTCAC TGAAACAGAC AAAGCAGGTC CTAATGGCTC CACAGCGTGT GCAAGCTACC 
AGCATCCCAG ACCTGGGGCA GGAGTATGGC TACAAGGCAG CTCAAGCCTG GCAGGAGGCA 
GCCAACAACT ATCAGAAACA GGTTGGACGT CTGGAGGAGA GTCTGAACCA GACAAAAGCT 
AACATGACAA AGATTTACCA GGAGAAGAGA GAGAGCCAGC GTCAGGTACA GCATCTGGCC 
AAAGAGCTGG AGAGCACGAG GATCAAGAGG CAGATGCTGG AGAAACATGC CATGCAGCAA 
AGAGAAGAAC AGAAAGAGGA GCTTCAACAT CTTCAGGCCC AAGTAAACAC CCTGGAGTTG 
GAGAAAGACT GTCTGGGGCA GCAGATTGAC AGCCTGATGT TGGATAGACA ACATCTGCTG 
CAGGTGAAGA TGTCTCTTGG ACTGGAGGTG GCAACATACA GAGCTTTGCT GGACAGCGAG 
GGCCTGAGAA TCGACAGACC AACACCAAAC AAGACCAGCT CTACTGTCTT TTTAGATGCA 
CTCTCAAAGC CCACTGGGAT CCAGTCAACT TCTCAAACCA CTGCTGCTTC CTGCCTTCTC 
AGCAGTTCTG TGTCTACAAG TCACAGATCA ATCGCCTCAT CCCGCAGTCT GCTGACCAGT 
GCGGCTCCGT CATGGACACT GACACGAGGG ACTCCACAGA GACCACCAAG CAGTACCTCG 
ATGACAGAAA AGACAGAGGA TCACATATCT GAGGAGACTA AGAGATCTGC TGAGGAGTCT 
GTGGATCAAT TACAGCAAGA GAAAGTCCAG CAAGACTGGA CTCTCGAATC CACTCTTCCC 
AAAACATCTG CAGAACCAAA ACCAGAGCTT CAACCAGAAG AAATTAAGGT GGAAGACGAA 
GCAAATGAAC CCCAACAAGC TCATATGGTT GAATCTAAAA CTGATGAATC TGAACTGACT 
AGTGTACCAG CTGAGCAACA AGGAAGTATG TCCCAAACTC CAGAGACAAA GAGCTGGGCT 
GGTCATTTCA ATGATCCTGC AGAAGTTTCA GAAGACGGAA AGGATGAAGA CACTGAAGTG 
TCTGTCGAAA TGGCTCGTAT CTCACATGCA CCCAAGGTTG CTTGGGAAGA AATCAAATCT 
GCAGTAGAGG ATGAAAAAGA TGATGCTTCT GAGATGGACG TAAGATCAGA GATCATCAGT 
GAAAGCCACA CATTTGCATA TGGAGATGCA GAGAATGATA GTAATACATT AACATCAAGC 
CATATCAGTC AAAACACCAA TGCACTGGCT TCATCTTTCC TTGAGCAAGG AACTCTAGAT 
TCAGCTAGTT ATTTTGGCTA TGAGGCAACA AATGAAAATC TTATGAAGGA AGAGGAACTG 
GACAATGTGA GCAATATTAG CGAGGAGGTG ACAGAACAAT TGAACAGTGA AACTGAGGCA 
GCTATTGATT CAATCAATGA GTGGGACAGA CAGGAAGAAA GTGAACCAGA GAATGAGACC 
AAAGTGATGA CTTCTTACTT TGAGGTAGAA GAGGGAGAAA TGAGCATCAA TACTGACACG 
AAAGATAAAA CTGAAGATGA CATAGAAAGG GAAGAGTTAG AAGTGACAGA AAGAGAAATT 
CTAGTGCAAA GTGCAGGTGG AATGCTGGGT GTAGATTTAC CCAATGACAC TGATCATCAG 
GATGAGCACA ATCTACCTCA GATAGAGCTG AACGAAGACC AATCTGAGGC AGAAGAAGAA 
AATCAAAAAG AAAAGCAATG TGATGAAGAT GACTCCCCAA ACATATCAGC CTCATTGAAA 
ACTGACCCAG GAGAAGGAGA CAGTTACAGC CAGGAGCACA CGCTGGCAGA CACACGGCCC 
TTGATCCGTT ATAAGAGCGA TGAGGAAACC TCACACCACA TCGGTGAGAC CAGTGACAGT 
GAGGATGAGA AAGAAAGAAT TGATCAAGGC CACTTGAATG AGAAACGTTT TAACACTATG 
GAGGATCTCA CTGAAGAACC AGACATGGAG GTCACCGGAA AGATGAGGCT GGAGGATCTT 
GTTTCTAATG AAGAAGCTGC AGCTGACGAT GTGGCATCTG TGATGTTTCA GAAAGTCTCT 
GGAGACCATG AAAGTTTGGA TGTGGTGGTG CAAGAAAGTG AACGCAAGGG TAATCTGGAG 
AAGGAAGATA GTGGTGATGC TGATAATATG AGAGACAATA AGGAAGAAGA TGTCAGTGTT 
TTTGAGCAAA ATGAGAACCA GCAACTGACT GAACACCAGC ATGTTCACAC AGAACAGGTT 
GAGGATAAAC CAGTCCATTC ACATGAAACT CAAGAACACG TCAACACGGA GTTTTCATCC 
ACATTTTCTG AAAGATCTCA GCAAGAGCAA CTGGAAGAAT CAAGTCTGAC TATGTTTGAA 
GATGAAGCTG CAACCAAAGA AGCAGAAGAT TCGGATGTCT CTATGCACAC CAACATAGAT 
CTGATTGACA GTCGCTCTTT AGAGAGCGAG ATAAACGGTC AGCCAGACAT TATGACCTCA 
GACATGGCCA ACTCTGAGTG CAATAGCTCA GAAGACGAGT CTCCAAATGC CAGCCAGTGC 
TTTCAGAACA CAAGCCTTTT AGCAGCGGCG ACTCCTAATG AACAGCCATT AACCTTTACT 
AATGAAGTCT CCAAGGCTGA TTATGTGTCT GACAACAACG ACGTGCCTGA AGAAGTGCTC 
AGTGAGGAGA AGAGCACTGA TGAGCCCAAG GCCTCTCAGA TTGATGACTT GGAGAATTTT 
AACATTTGGG GGGAAAGCAC GAGCATTTCA GATGCAGCCC AGACCACACA TGCAAGCATG 
GATGAACATT CAAGCATTTC CCCAGTGAAT GAGAATCTGG AAAGCTCAAA CAAAATACCA 
AGTGTGGCGG AAAACATCTT TGGACATTTT GAAGAACATC TCGAAAGGTC AGTGGAGAGC 
TTTCCAGAAA AAGAAGTGAC TGTGATGGAC TTTGAGAACA ACGATTTAGG AGAGGAATTT 
CAGTCGAAGC AAATGAAAAG TGAGATTCAC AGCTTCTTCA GCACTAGTTT GAAGCAAGAT 
TTCTGGAGCG AAGGGAAAAT GGAGATGGCA GCTACTTATG ATCCCGCCAA AACTGAAGAC 
TTAAATCAGG CCATGGTCTT TGGAGAGGAG TGGAGAGAAA TAGGAGTGCA ATCAGCGAAT 
GGAGATACTA AAGAAGAGAT GGAGATCCTC AAAATCAGGG ATGATAAGCA AAAAGATGGA 
CAGCCAGCGC AGAGCAAGAT AGTCCAGTCA GATGATTCTG CTGATGAGGG GGATTCCTGG 
TCATCTGGTG ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nes ( XM_001919887.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001919922.1 Danio rerio nestin (nes), mRNA.

Price & Availability↑

CloneID	ODa21583
Clone ID Related Accession (Same CDS sequence)	XM_001919887.6 , XM_001919887.7
Accession Version	XM_001919887.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nestin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919887.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTC TGGGTGTCCG ACAGCCATTT GGCCCTCAGT TTCAGGAGGA AAAGTACCAG 
ATGCTAGAGC TGAACCACCG CCTTGAATCA TACTTGGGCC GTGTGAAACT TCTGGAGGAA 
GAGAACAAGC TACTTCGAGA GGAGATCCAC ACTCTCAAGA GCAGCAGGGA CCCAGCAGGC 
CAACGTAAAG CCCAGGAGGA GGCTCTCAGT CAGGCCAGGA GGATGATGGA GGAGGCCTGG 
AGAAAGAAAG ACTGTGTGGA GCTGGAGGTA GAGAATCTGA TGGAGGATAT GGAGAAGGTG 
AGCTTTCAAA GAAAGAAGGT GAAGACTGCA CAGGCTGAAG CTCAGAGGAA GCTCACAGAG 
AGCAGAAAGG AGCTGGAAGA GGAGCGCAGG GCTCAGATTT GGCTTAGAGA GAAGGTCGGT 
CAACTCGAGA AAGACCTCAT GCTGCAGATG CAAGTGCACC AGGAGAACAT GGAAACCATG 
CAGGCCTCAC TGAAACAGAC AAAGCAGGTC CTAATGGCTC CACAGCGTGT GCAAGCTACC 
AGCATCCCAG ACCTGGGGCA GGAGTATGGC TACAAGGCAG CTCAAGCCTG GCAGGAGGCA 
GCCAACAACT ATCAGAAACA GGTTGGACGT CTGGAGGAGA GTCTGAACCA GACAAAAGCT 
AACATGACAA AGATTTACCA GGAGAAGAGA GAGAGCCAGC GTCAGGTACA GCATCTGGCC 
AAAGAGCTGG AGAGCACGAG GATCAAGAGG CAGATGCTGG AGAAACATGC CATGCAGCAA 
AGAGAAGAAC AGAAAGAGGA GCTTCAACAT CTTCAGGCCC AAGTAAACAC CCTGGAGTTG 
GAGAAAGACT GTCTGGGGCA GCAGATTGAC AGCCTGATGT TGGATAGACA ACATCTGCTG 
CAGGTGAAGA TGTCTCTTGG ACTGGAGGTG GCAACATACA GAGCTTTGCT GGACAGCGAG 
GGCCTGAGAA TCGACAGACC AACACCAAAC AAGACCAGCT CTACTGTCTT TTTAGATGCA 
CTCTCAAAGC CCACTGGGAT CCAGTCAACT TCTCAAACCA CTGCTGCTTC CTGCCTTCTC 
AGCAGTTCTG TGTCTACAAG TCACAGATCA ATCGCCTCAT CCCGCAGTCT GCTGACCAGT 
GCGGCTCCGT CATGGACACT GACACGAGGG ACTCCACAGA GACCACCAAG CAGTACCTCG 
ATGACAGAAA AGACAGAGGA TCACATATCT GAGGAGACTA AGAGATCTGC TGAGGAGTCT 
GTGGATCAAT TACAGCAAGA GAAAGTCCAG CAAGACTGGA CTCTCGAATC CACTCTTCCC 
AAAACATCTG CAGAACCAAA ACCAGAGCTT CAACCAGAAG AAATTAAGGT GGAAGACGAA 
GCAAATGAAC CCCAACAAGC TCATATGGTT GAATCTAAAA CTGATGAATC TGAACTGACT 
AGTGTACCAG CTGAGCAACA AGGAAGTATG TCCCAAACTC CAGAGACAAA GAGCTGGGCT 
GGTCATTTCA ATGATCCTGC AGAAGTTTCA GAAGACGGAA AGGATGAAGA CACTGAAGTG 
TCTGTCGAAA TGGCTCGTAT CTCACATGCA CCCAAGGTTG CTTGGGAAGA AATCAAATCT 
GCAGTAGAGG ATGAAAAAGA TGATGCTTCT GAGATGGACG TAAGATCAGA GATCATCAGT 
GAAAGCCACA CATTTGCATA TGGAGATGCA GAGAATGATA GTAATACATT AACATCAAGC 
CATATCAGTC AAAACACCAA TGCACTGGCT TCATCTTTCC TTGAGCAAGG AACTCTAGAT 
TCAGCTAGTT ATTTTGGCTA TGAGGCAACA AATGAAAATC TTATGAAGGA AGAGGAACTG 
GACAATGTGA GCAATATTAG CGAGGAGGTG ACAGAACAAT TGAACAGTGA AACTGAGGCA 
GCTATTGATT CAATCAATGA GTGGGACAGA CAGGAAGAAA GTGAACCAGA GAATGAGACC 
AAAGTGATGA CTTCTTACTT TGAGGTAGAA GAGGGAGAAA TGAGCATCAA TACTGACACG 
AAAGATAAAA CTGAAGATGA CATAGAAAGG GAAGAGTTAG AAGTGACAGA AAGAGAAATT 
CTAGTGCAAA GTGCAGGTGG AATGCTGGGT GTAGATTTAC CCAATGACAC TGATCATCAG 
GATGAGCACA ATCTACCTCA GATAGAGCTG AACGAAGACC AATCTGAGGC AGAAGAAGAA 
AATCAAAAAG AAAAGCAATG TGATGAAGAT GACTCCCCAA ACATATCAGC CTCATTGAAA 
ACTGACCCAG GAGAAGGAGA CAGTTACAGC CAGGAGCACA CGCTGGCAGA CACACGGCCC 
TTGATCCGTT ATAAGAGCGA TGAGGAAACC TCACACCACA TCGGTGAGAC CAGTGACAGT 
GAGGATGAGA AAGAAAGAAT TGATCAAGGC CACTTGAATG AGAAACGTTT TAACACTATG 
GAGGATCTCA CTGAAGAACC AGACATGGAG GTCACCGGAA AGATGAGGCT GGAGGATCTT 
GTTTCTAATG AAGAAGCTGC AGCTGACGAT GTGGCATCTG TGATGTTTCA GAAAGTCTCT 
GGAGACCATG AAAGTTTGGA TGTGGTGGTG CAAGAAAGTG AACGCAAGGG TAATCTGGAG 
AAGGAAGATA GTGGTGATGC TGATAATATG AGAGACAATA AGGAAGAAGA TGTCAGTGTT 
TTTGAGCAAA ATGAGAACCA GCAACTGACT GAACACCAGC ATGTTCACAC AGAACAGGTT 
GAGGATAAAC CAGTCCATTC ACATGAAACT CAAGAACACG TCAACACGGA GTTTTCATCC 
ACATTTTCTG AAAGATCTCA GCAAGAGCAA CTGGAAGAAT CAAGTCTGAC TATGTTTGAA 
GATGAAGCTG CAACCAAAGA AGCAGAAGAT TCGGATGTCT CTATGCACAC CAACATAGAT 
CTGATTGACA GTCGCTCTTT AGAGAGCGAG ATAAACGGTC AGCCAGACAT TATGACCTCA 
GACATGGCCA ACTCTGAGTG CAATAGCTCA GAAGACGAGT CTCCAAATGC CAGCCAGTGC 
TTTCAGAACA CAAGCCTTTT AGCAGCGGCG ACTCCTAATG AACAGCCATT AACCTTTACT 
AATGAAGTCT CCAAGGCTGA TTATGTGTCT GACAACAACG ACGTGCCTGA AGAAGTGCTC 
AGTGAGGAGA AGAGCACTGA TGAGCCCAAG GCCTCTCAGA TTGATGACTT GGAGAATTTT 
AACATTTGGG GGGAAAGCAC GAGCATTTCA GATGCAGCCC AGACCACACA TGCAAGCATG 
GATGAACATT CAAGCATTTC CCCAGTGAAT GAGAATCTGG AAAGCTCAAA CAAAATACCA 
AGTGTGGCGG AAAACATCTT TGGACATTTT GAAGAACATC TCGAAAGGTC AGTGGAGAGC 
TTTCCAGAAA AAGAAGTGAC TGTGATGGAC TTTGAGAACA ACGATTTAGG AGAGGAATTT 
CAGTCGAAGC AAATGAAAAG TGAGATTCAC AGCTTCTTCA GCACTAGTTT GAAGCAAGAT 
TTCTGGAGCG AAGGGAAAAT GGAGATGGCA GCTACTTATG ATCCCGCCAA AACTGAAGAC 
TTAAATCAGG CCATGGTCTT TGGAGAGGAG TGGAGAGAAA TAGGAGTGCA ATCAGCGAAT 
GGAGATACTA AAGAAGAGAT GGAGATCCTC AAAATCAGGG ATGATAAGCA AAAAGATGGA 
CAGCCAGCGC AGAGCAAGAT AGTCCAGTCA GATGATTCTG CTGATGAGGG GGATTCCTGG 
TCATCTGGTG ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919922.1
CDS	183..3980
Translation	MELLGVRQPFGPQFQEEKYQMLELNHRLESYLGRVKLLEEENKLLREEIHTLKSSRDPAGQRKAQEEALSQARRMMEEAWRKKDCVELEVENLMEDMEKVSFQRKKVKTAQAEAQRKLTESRKELEEERRAQIWLREKVGQLEKDLMLQMQVHQENMETMQASLKQTKQVLMAPQRVQATSIPDLGQEYGYKAAQAWQEAANNYQKQVGRLEESLNQTKANMTKIYQEKRESQRQVQHLAKELESTRIKRQMLEKHAMQQREEQKEELQHLQAQVNTLELEKDCLGQQIDSLMLDRQHLLQVKMSLGLEVATYRALLDSEGLRIDRPTPNKTSSTVFLDALSKPTGIQSTSQTTAASCLLSSSVSTSHRSIASSRSLLTSAAPSWTLTRGTPQRPPSSTSMTEKTEDHISEETKRSAEESVDQLQQEKVQQDWTLESTLPKTSAEPKPELQPEEIKVEDEANEPQQAHMVESKTDESELTSVPAEQQGSMSQTPETKSWAGHFNDPAEVSEDGKDEDTEVSVEMARISHAPKVAWEEIKSAVEDEKDDASEMDVRSEIISESHTFAYGDAENDSNTLTSSHISQNTNALASSFLEQGTLDSASYFGYEATNENLMKEEELDNVSNISEEVTEQLNSETEAAIDSINEWDRQEESEPENETKVMTSYFEVEEGEMSINTDTKDKTEDDIEREELEVTEREILVQSAGGMLGVDLPNDTDHQDEHNLPQIELNEDQSEAEEENQKEKQCDEDDSPNISASLKTDPGEGDSYSQEHTLADTRPLIRYKSDEETSHHIGETSDSEDEKERIDQGHLNEKRFNTMEDLTEEPDMEVTGKMRLEDLVSNEEAAADDVASVMFQKVSGDHESLDVVVQESERKGNLEKEDSGDADNMRDNKEEDVSVFEQNENQQLTEHQHVHTEQVEDKPVHSHETQEHVNTEFSSTFSERSQQEQLEESSLTMFEDEAATKEAEDSDVSMHTNIDLIDSRSLESEINGQPDIMTSDMANSECNSSEDESPNASQCFQNTSLLAAATPNEQPLTFTNEVSKADYVSDNNDVPEEVLSEEKSTDEPKASQIDDLENFNIWGESTSISDAAQTTHASMDEHSSISPVNENLESSNKIPSVAENIFGHFEEHLERSVESFPEKEVTVMDFENNDLGEEFQSKQMKSEIHSFFSTSLKQDFWSEGKMEMAATYDPAKTEDLNQAMVFGEEWREIGVQSANGDTKEEMEILKIRDDKQKDGQPAQSKIVQSDDSADEGDSWSSGDE

Target ORF information:

RefSeq Version	XM_001919887.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nestin (nes), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919887.7

ORF Insert Sequence:

ATGGAGCTTC TGGGTGTCCG ACAGCCATTT GGCCCTCAGT TTCAGGAGGA AAAGTACCAG 
ATGCTAGAGC TGAACCACCG CCTTGAATCA TACTTGGGCC GTGTGAAACT TCTGGAGGAA 
GAGAACAAGC TACTTCGAGA GGAGATCCAC ACTCTCAAGA GCAGCAGGGA CCCAGCAGGC 
CAACGTAAAG CCCAGGAGGA GGCTCTCAGT CAGGCCAGGA GGATGATGGA GGAGGCCTGG 
AGAAAGAAAG ACTGTGTGGA GCTGGAGGTA GAGAATCTGA TGGAGGATAT GGAGAAGGTG 
AGCTTTCAAA GAAAGAAGGT GAAGACTGCA CAGGCTGAAG CTCAGAGGAA GCTCACAGAG 
AGCAGAAAGG AGCTGGAAGA GGAGCGCAGG GCTCAGATTT GGCTTAGAGA GAAGGTCGGT 
CAACTCGAGA AAGACCTCAT GCTGCAGATG CAAGTGCACC AGGAGAACAT GGAAACCATG 
CAGGCCTCAC TGAAACAGAC AAAGCAGGTC CTAATGGCTC CACAGCGTGT GCAAGCTACC 
AGCATCCCAG ACCTGGGGCA GGAGTATGGC TACAAGGCAG CTCAAGCCTG GCAGGAGGCA 
GCCAACAACT ATCAGAAACA GGTTGGACGT CTGGAGGAGA GTCTGAACCA GACAAAAGCT 
AACATGACAA AGATTTACCA GGAGAAGAGA GAGAGCCAGC GTCAGGTACA GCATCTGGCC 
AAAGAGCTGG AGAGCACGAG GATCAAGAGG CAGATGCTGG AGAAACATGC CATGCAGCAA 
AGAGAAGAAC AGAAAGAGGA GCTTCAACAT CTTCAGGCCC AAGTAAACAC CCTGGAGTTG 
GAGAAAGACT GTCTGGGGCA GCAGATTGAC AGCCTGATGT TGGATAGACA ACATCTGCTG 
CAGGTGAAGA TGTCTCTTGG ACTGGAGGTG GCAACATACA GAGCTTTGCT GGACAGCGAG 
GGCCTGAGAA TCGACAGACC AACACCAAAC AAGACCAGCT CTACTGTCTT TTTAGATGCA 
CTCTCAAAGC CCACTGGGAT CCAGTCAACT TCTCAAACCA CTGCTGCTTC CTGCCTTCTC 
AGCAGTTCTG TGTCTACAAG TCACAGATCA ATCGCCTCAT CCCGCAGTCT GCTGACCAGT 
GCGGCTCCGT CATGGACACT GACACGAGGG ACTCCACAGA GACCACCAAG CAGTACCTCG 
ATGACAGAAA AGACAGAGGA TCACATATCT GAGGAGACTA AGAGATCTGC TGAGGAGTCT 
GTGGATCAAT TACAGCAAGA GAAAGTCCAG CAAGACTGGA CTCTCGAATC CACTCTTCCC 
AAAACATCTG CAGAACCAAA ACCAGAGCTT CAACCAGAAG AAATTAAGGT GGAAGACGAA 
GCAAATGAAC CCCAACAAGC TCATATGGTT GAATCTAAAA CTGATGAATC TGAACTGACT 
AGTGTACCAG CTGAGCAACA AGGAAGTATG TCCCAAACTC CAGAGACAAA GAGCTGGGCT 
GGTCATTTCA ATGATCCTGC AGAAGTTTCA GAAGACGGAA AGGATGAAGA CACTGAAGTG 
TCTGTCGAAA TGGCTCGTAT CTCACATGCA CCCAAGGTTG CTTGGGAAGA AATCAAATCT 
GCAGTAGAGG ATGAAAAAGA TGATGCTTCT GAGATGGACG TAAGATCAGA GATCATCAGT 
GAAAGCCACA CATTTGCATA TGGAGATGCA GAGAATGATA GTAATACATT AACATCAAGC 
CATATCAGTC AAAACACCAA TGCACTGGCT TCATCTTTCC TTGAGCAAGG AACTCTAGAT 
TCAGCTAGTT ATTTTGGCTA TGAGGCAACA AATGAAAATC TTATGAAGGA AGAGGAACTG 
GACAATGTGA GCAATATTAG CGAGGAGGTG ACAGAACAAT TGAACAGTGA AACTGAGGCA 
GCTATTGATT CAATCAATGA GTGGGACAGA CAGGAAGAAA GTGAACCAGA GAATGAGACC 
AAAGTGATGA CTTCTTACTT TGAGGTAGAA GAGGGAGAAA TGAGCATCAA TACTGACACG 
AAAGATAAAA CTGAAGATGA CATAGAAAGG GAAGAGTTAG AAGTGACAGA AAGAGAAATT 
CTAGTGCAAA GTGCAGGTGG AATGCTGGGT GTAGATTTAC CCAATGACAC TGATCATCAG 
GATGAGCACA ATCTACCTCA GATAGAGCTG AACGAAGACC AATCTGAGGC AGAAGAAGAA 
AATCAAAAAG AAAAGCAATG TGATGAAGAT GACTCCCCAA ACATATCAGC CTCATTGAAA 
ACTGACCCAG GAGAAGGAGA CAGTTACAGC CAGGAGCACA CGCTGGCAGA CACACGGCCC 
TTGATCCGTT ATAAGAGCGA TGAGGAAACC TCACACCACA TCGGTGAGAC CAGTGACAGT 
GAGGATGAGA AAGAAAGAAT TGATCAAGGC CACTTGAATG AGAAACGTTT TAACACTATG 
GAGGATCTCA CTGAAGAACC AGACATGGAG GTCACCGGAA AGATGAGGCT GGAGGATCTT 
GTTTCTAATG AAGAAGCTGC AGCTGACGAT GTGGCATCTG TGATGTTTCA GAAAGTCTCT 
GGAGACCATG AAAGTTTGGA TGTGGTGGTG CAAGAAAGTG AACGCAAGGG TAATCTGGAG 
AAGGAAGATA GTGGTGATGC TGATAATATG AGAGACAATA AGGAAGAAGA TGTCAGTGTT 
TTTGAGCAAA ATGAGAACCA GCAACTGACT GAACACCAGC ATGTTCACAC AGAACAGGTT 
GAGGATAAAC CAGTCCATTC ACATGAAACT CAAGAACACG TCAACACGGA GTTTTCATCC 
ACATTTTCTG AAAGATCTCA GCAAGAGCAA CTGGAAGAAT CAAGTCTGAC TATGTTTGAA 
GATGAAGCTG CAACCAAAGA AGCAGAAGAT TCGGATGTCT CTATGCACAC CAACATAGAT 
CTGATTGACA GTCGCTCTTT AGAGAGCGAG ATAAACGGTC AGCCAGACAT TATGACCTCA 
GACATGGCCA ACTCTGAGTG CAATAGCTCA GAAGACGAGT CTCCAAATGC CAGCCAGTGC 
TTTCAGAACA CAAGCCTTTT AGCAGCGGCG ACTCCTAATG AACAGCCATT AACCTTTACT 
AATGAAGTCT CCAAGGCTGA TTATGTGTCT GACAACAACG ACGTGCCTGA AGAAGTGCTC 
AGTGAGGAGA AGAGCACTGA TGAGCCCAAG GCCTCTCAGA TTGATGACTT GGAGAATTTT 
AACATTTGGG GGGAAAGCAC GAGCATTTCA GATGCAGCCC AGACCACACA TGCAAGCATG 
GATGAACATT CAAGCATTTC CCCAGTGAAT GAGAATCTGG AAAGCTCAAA CAAAATACCA 
AGTGTGGCGG AAAACATCTT TGGACATTTT GAAGAACATC TCGAAAGGTC AGTGGAGAGC 
TTTCCAGAAA AAGAAGTGAC TGTGATGGAC TTTGAGAACA ACGATTTAGG AGAGGAATTT 
CAGTCGAAGC AAATGAAAAG TGAGATTCAC AGCTTCTTCA GCACTAGTTT GAAGCAAGAT 
TTCTGGAGCG AAGGGAAAAT GGAGATGGCA GCTACTTATG ATCCCGCCAA AACTGAAGAC 
TTAAATCAGG CCATGGTCTT TGGAGAGGAG TGGAGAGAAA TAGGAGTGCA ATCAGCGAAT 
GGAGATACTA AAGAAGAGAT GGAGATCCTC AAAATCAGGG ATGATAAGCA AAAAGATGGA 
CAGCCAGCGC AGAGCAAGAT AGTCCAGTCA GATGATTCTG CTGATGAGGG GGATTCCTGG 
TCATCTGGTG ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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nes cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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