ptpn21 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptpn21
Entrez Gene ID	799627
Full Name	protein tyrosine phosphatase, non-receptor type 21
Synonyms	pez,ptpn14
General protein information	Preferred Names protein tyrosine phosphatase, non-receptor type 21 Names tyrosine-protein phosphatase non-receptor type 21 protein-tyrosine phosphatase D1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001339926.7	XP_001339962.4	tyrosine-protein phosphatase non-receptor type 21 isoform X2
XM_005169788.3	XP_005169845.1	tyrosine-protein phosphatase non-receptor type 21 isoform X1
XM_005169788.4	XP_005169845.1	tyrosine-protein phosphatase non-receptor type 21 isoform X1
XM_001339926.8	XP_001339962.4	tyrosine-protein phosphatase non-receptor type 21 isoform X2

Pathways from BioSystems

Homologies

	PTPN21	XP_001083418.1
Canis lupus familiaris (dog)	PTPN21	XP_547945.2
Pan troglodytes (chimpanzee)	PTPN21	XP_510107.2
Bos taurus (cattle)	PTPN21	XP_002691133.1
Gallus gallus (chicken)	PTPN21	XP_421308.3
Danio rerio (zebrafish)	ptpn21	XP_001339962.4
Mus musculus (house mouse)	Ptpn21	NP_036007.2
Rattus norvegicus (Norway rat)	Ptpn21	NP_598229.1
Homo sapiens (human)	PTPN21	NP_008970.2
Xenopus tropicalis (tropical clawed frog)	ptpn21	NP_001106507.1

Gene Ontology

The following ptpn21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptpn21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22135	XM_005169788.4 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa22136	XM_001339926.8 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa22135	XM_005169788.3	Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa22136	XM_001339926.7	Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptpn21 ( XM_005169788.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169845.1 Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22135
Clone ID Related Accession (Same CDS sequence)	XM_005169788.3 , XM_005169788.4
Accession Version	XM_005169788.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 21 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169788.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTTC CGTTCGGATT AAAGCTCAAG AGAACCAGGA GATACACGGT GTCCAGCAAG 
AGCTGCCTGG TGACCCGCAT TCAGCTGCTC AATGGAGAAT ACGTGGAGTT CACGCTTTCG 
GTGGAGAGTA CGGGACAGGA GTGTCTGGAG GCGGTCGCGC AGAGGCTGGA GCTACGCGAG 
ATAACGTACT TCAGCCTGTG GTATTACAAC AAGCAGAACC AGCAGAGATG GATTGATCTG 
GAGAAGCCGC TGAAGAAGCA GCTGGACAAG TACGGACTGG AGCCCACCGT TTACTTTGGG 
GTGGTGTTTT ACGTCTGCAC CGTCACCCAA CTACAACAAG AAATAACCAG ATATCAGTAT 
TATCTGCAGC TGAAGAAAGA TGTTCTGGAG GGAAAGATCC CCTGCTCTAT TGAGCAAGCC 
ATTCATCTGG CCAGCCTGGC TGTGCAAGCT GACCTCGGAG ACTTCAATCG GTATGATTCC 
CAGGAATTCC TTCAAAAAAT CGTGCTTTTT CCGATGCCAT GGATACAAGA CGAGAGGGTT 
GTGGAAGAAG CCACTCAGAA AGTCACCATG CTTTATCAGA ACTACCGAGG TCTTTCTGCA 
CCGGAGGCTG AGCTACTGTA CATGCAGGAG GTGGAGAAGA TTGAGGGCTA CGGCGAGGAG 
AGCATCCAAG CCAAGGACTC TCAGGCCACA GACGTGATCA TCGGCTCCTG TCTGGATGGG 
ATTTTTGTCA AGCACAAAAA TGGGAAAGCT CCTCTCATAT TTCGGTGGAA TGACATTAAT 
AATATGACTC ATAATAAGTC CTTCTTTGCC CTGGAGCTCG CCAACAAAGA AGACACAATC 
CAGTTCCAGA CCGAGGACAT GGAGACGTCC AAATATGTGT GCAGAATGTG GCTGGCGAGG 
CACAAGTTTT ACAAGATTAA CAACAGCAGT CTAGAGCCGT CTTGTGACCC GAACTCCGAG 
GGCCCTCAAA GGTCCATTCT CTCTCTTTCA TTTCCTCGCT TTCCAGCCCT CTCTCGTCTC 
TCTGTGCCTG CTGATAAAGG ACAAACTCAG CCAGCTCCTG TAAACCCAGT GAGGCGACGG 
TCCTCCACAA GAATGTCTCT GCCAAAACCG CAGCCCTACA TGATGCCACC TCAGATGCAC 
TTTAACGGCC ACTATAACGA ACCGTACACG TCTTCACAAG ATAATCTCTA CATCAACAAC 
CAGAATGGTT TCTACTACCA TTCCCAAACC AGCCTGGACC GCTCTCCGCA CGAGTACAAC 
GGACGCATCC GAAACGGCAG CGTTTACAGC GCACAGAGCA CCAGCTCCCT GAACAACCCA 
CAGCACTACC TGCAGCCATC GCCGATGTCC TCCAACCCAA GCATCACAGG AAGTGACCTC 
ATGCGTCCCG ACTACGTGCC CTCCCACCGG CACAGCGCCC TGATTCCACC GTCCTACCGC 
GCCACGCCAG ATTACGAAAC CGTGATGCGA CAGAAAAACA TGATTCCAGC TGCAGAGCGC 
CAGAGCCACT CCATGAGGAA CCTAAATATC GGAAACTCGT ACGCTTACAG CCGACCAGAT 
CCGCTAGTGT ACAGCCAACC GGAAATCCGA GAGCACGGCG CAGGACAGTA TCCTTTTCAC 
CTTAATTACA GCTTCCACAG TCCATCTCCA TACCCCTATC CCACTGAGAG ACGGCCTGTA 
GTGGGCGCTG TAAGCGTTCC CGAACTCACC AACGTCCAGC TGCAGCAGGC GCAGGAATAT 
CCGCCGCCGA ACATCATCAG GAATCAGGTG TACTGGCCTC CGCCGCCGTA TCCGTACCCT 
CATCCTCGAC CCGCCAACAG CACCCCGGAT CTGTCCCGGC ACCTGTACGT CAGCAGCAGT 
AACCCGGATC TGATAACGCG CCGCGTGCAT CACTCGGTGC AGACCTTTCA GGAGGACAGC 
CTGCCAGTGG CGCACTCACT ACAGGAGGTT AGCGAACCGC TAGTCACAGC ACGCCGTCCA 
CACATGCACA AACGCAACTC AATCGAGATC GCAGGACTGG CCTACGGACT GGAGAACATG 
AGGCTCAAGG AGAGGAAGGT ATCCGCATCA GCAGCTGATA CTCCGCCCCC TGTTGCCGCG 
GCAGTGCCCC ATGCAGCATC CGGCTCCGAC ATCAACGTGG TCCTAGAGGT GTCGAAACCA 
GAAGACGTAG GCATCAAAGA GGATGTTTTC TACGGCCACA AGAAGTCTCT GTCGGATGCC 
ACCATGCTGG TGCACAGCAG CGGAGAGGAG GAGGAGTTTG AGGACGACAG CGGCAGACAC 
ACACCACAGT CTCAGGACGC AGTGAGCGGC CCAGAGCATC CCATGACCTC GCTGGGACGC 
TCGCTCGGAG ATCCTCCTGC TTATCCCTTC AGCCACAGTA TGGAGACGGT GCTAACAAGT 
CCTCCGGCCT ACCAGGCGCA TTCCATCATG CAGAAAGCAG AGGACGTGGG GCCACTGCCG 
CCTCTGAGGG AGCCCGGACT CATGATGCCC TCAGTGTCAG AGGGAGATCT GAGCGGACGC 
TTAAGGAAAA ATAGAGACAT CTCGAAAAGA CCTGTGTCTG ACGTGCCTCC GGGGAGGAGA 
AACATTGATG GGCTGCCACC CGCGGGAATG AAAAAAGGAC GCTCGGAGGT GAAGAAGGTC 
GGTCCGCTGA AGCTGGCAGC TCTGAACGGT TTGACCCTGT CCAGGATTCC TCTGCCGGAT 
GATGGAAAGG ACGACCCGGA AAGCGCCTCT AATGATGAAC GGTGTAAGTT TCTGGAGCAG 
CGTTTGGATC AGGGGATGGT GTTCACGGAG TACGAGCAGG TGCCCAAGAA ACGACCCAAC 
AGTGAGTACA GCATCGCTCA GATGCCCGAG AACAACGACA GAAACCGCTT TCAGGACGTC 
CTGCCCTACG ACGACACACG TGTGGAGCTG GTGCCCACCA AAGAGAACAA CACAGGCTAC 
ATCAACGCCT CGCACATCAG GGTGACGGTC AGAGGTAAAG AGTGGAGTTA TATTGCGTCT 
CAGGGTCCGC TTTCCAGCAC CTGTCAGGAT TTCTGGCAGA TGGTTTGGGA ACAAGGAGTG 
TCCATCATCG CAATGGTTAC AGCAGAGGAG GAAGGAGGCC GTGAGAAGAG TTTCCGCTAC 
TGGCCTCGTC TGGGCTCTCG ACACAACACT GTTACATACG GACGCTTCAA GATCACCACA 
CGCTTCCGCA CAGACTCGGG CTGCTACGCC ACCACCGGCC TGAAGATCAA GCATCTACTG 
ACGGGGCAGG AGAGGACCGT CTGGCACCTG CAGTACACCG ACTGGCCCGA TCACGGCTGC 
CCTGAGGACT TTAAAGGCTT TCTCTCGTAT TTGGAGGAGA TCCAGTCTGT GAGGCGACAC 
ACCAACAGTT CCAGCGACCC AAAGAACACA AACCTGCCGG TGCTGGTGCA CTGCAGCGCG 
GGCGTGGGAC GCACCGGTGT GGTCATCCTG TCCGAGATCA TGATCGCCTG TCTGGAACAC 
AATGAGATGC TGGACGTCCC GACGGTTCTG AACCTGCTCC GGCAGCAGCG AATGATGATG 
GTACAGACAC TCTCACAATA CACCTTCATC TACAAAGTCC TCATTCAGTT CCTGAAAAAC 
TCCAGACTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169845.1
CDS	301..3915
Translation	MPLPFGLKLKRTRRYTVSSKSCLVTRIQLLNGEYVEFTLSVESTGQECLEAVAQRLELREITYFSLWYYNKQNQQRWIDLEKPLKKQLDKYGLEPTVYFGVVFYVCTVTQLQQEITRYQYYLQLKKDVLEGKIPCSIEQAIHLASLAVQADLGDFNRYDSQEFLQKIVLFPMPWIQDERVVEEATQKVTMLYQNYRGLSAPEAELLYMQEVEKIEGYGEESIQAKDSQATDVIIGSCLDGIFVKHKNGKAPLIFRWNDINNMTHNKSFFALELANKEDTIQFQTEDMETSKYVCRMWLARHKFYKINNSSLEPSCDPNSEGPQRSILSLSFPRFPALSRLSVPADKGQTQPAPVNPVRRRSSTRMSLPKPQPYMMPPQMHFNGHYNEPYTSSQDNLYINNQNGFYYHSQTSLDRSPHEYNGRIRNGSVYSAQSTSSLNNPQHYLQPSPMSSNPSITGSDLMRPDYVPSHRHSALIPPSYRATPDYETVMRQKNMIPAAERQSHSMRNLNIGNSYAYSRPDPLVYSQPEIREHGAGQYPFHLNYSFHSPSPYPYPTERRPVVGAVSVPELTNVQLQQAQEYPPPNIIRNQVYWPPPPYPYPHPRPANSTPDLSRHLYVSSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLVTARRPHMHKRNSIEIAGLAYGLENMRLKERKVSASAADTPPPVAAAVPHAASGSDINVVLEVSKPEDVGIKEDVFYGHKKSLSDATMLVHSSGEEEEFEDDSGRHTPQSQDAVSGPEHPMTSLGRSLGDPPAYPFSHSMETVLTSPPAYQAHSIMQKAEDVGPLPPLREPGLMMPSVSEGDLSGRLRKNRDISKRPVSDVPPGRRNIDGLPPAGMKKGRSEVKKVGPLKLAALNGLTLSRIPLPDDGKDDPESASNDERCKFLEQRLDQGMVFTEYEQVPKKRPNSEYSIAQMPENNDRNRFQDVLPYDDTRVELVPTKENNTGYINASHIRVTVRGKEWSYIASQGPLSSTCQDFWQMVWEQGVSIIAMVTAEEEGGREKSFRYWPRLGSRHNTVTYGRFKITTRFRTDSGCYATTGLKIKHLLTGQERTVWHLQYTDWPDHGCPEDFKGFLSYLEEIQSVRRHTNSSSDPKNTNLPVLVHCSAGVGRTGVVILSEIMIACLEHNEMLDVPTVLNLLRQQRMMMVQTLSQYTFIYKVLIQFLKNSRLI

Target ORF information:

RefSeq Version	XM_005169788.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169788.3

ORF Insert Sequence:

ATGCCCCTTC CGTTCGGATT AAAGCTCAAG AGAACCAGGA GATACACGGT GTCCAGCAAG 
AGCTGCCTGG TGACCCGCAT TCAGCTGCTC AATGGAGAAT ACGTGGAGTT CACGCTTTCG 
GTGGAGAGTA CGGGACAGGA GTGTCTGGAG GCGGTCGCGC AGAGGCTGGA GCTACGCGAG 
ATAACGTACT TCAGCCTGTG GTATTACAAC AAGCAGAACC AGCAGAGATG GATTGATCTG 
GAGAAGCCGC TGAAGAAGCA GCTGGACAAG TACGGACTGG AGCCCACCGT TTACTTTGGG 
GTGGTGTTTT ACGTCTGCAC CGTCACCCAA CTACAACAAG AAATAACCAG ATATCAGTAT 
TATCTGCAGC TGAAGAAAGA TGTTCTGGAG GGAAAGATCC CCTGCTCTAT TGAGCAAGCC 
ATTCATCTGG CCAGCCTGGC TGTGCAAGCT GACCTCGGAG ACTTCAATCG GTATGATTCC 
CAGGAATTCC TTCAAAAAAT CGTGCTTTTT CCGATGCCAT GGATACAAGA CGAGAGGGTT 
GTGGAAGAAG CCACTCAGAA AGTCACCATG CTTTATCAGA ACTACCGAGG TCTTTCTGCA 
CCGGAGGCTG AGCTACTGTA CATGCAGGAG GTGGAGAAGA TTGAGGGCTA CGGCGAGGAG 
AGCATCCAAG CCAAGGACTC TCAGGCCACA GACGTGATCA TCGGCTCCTG TCTGGATGGG 
ATTTTTGTCA AGCACAAAAA TGGGAAAGCT CCTCTCATAT TTCGGTGGAA TGACATTAAT 
AATATGACTC ATAATAAGTC CTTCTTTGCC CTGGAGCTCG CCAACAAAGA AGACACAATC 
CAGTTCCAGA CCGAGGACAT GGAGACGTCC AAATATGTGT GCAGAATGTG GCTGGCGAGG 
CACAAGTTTT ACAAGATTAA CAACAGCAGT CTAGAGCCGT CTTGTGACCC GAACTCCGAG 
GGCCCTCAAA GGTCCATTCT CTCTCTTTCA TTTCCTCGCT TTCCAGCCCT CTCTCGTCTC 
TCTGTGCCTG CTGATAAAGG ACAAACTCAG CCAGCTCCTG TAAACCCAGT GAGGCGACGG 
TCCTCCACAA GAATGTCTCT GCCAAAACCG CAGCCCTACA TGATGCCACC TCAGATGCAC 
TTTAACGGCC ACTATAACGA ACCGTACACG TCTTCACAAG ATAATCTCTA CATCAACAAC 
CAGAATGGTT TCTACTACCA TTCCCAAACC AGCCTGGACC GCTCTCCGCA CGAGTACAAC 
GGACGCATCC GAAACGGCAG CGTTTACAGC GCACAGAGCA CCAGCTCCCT GAACAACCCA 
CAGCACTACC TGCAGCCATC GCCGATGTCC TCCAACCCAA GCATCACAGG AAGTGACCTC 
ATGCGTCCCG ACTACGTGCC CTCCCACCGG CACAGCGCCC TGATTCCACC GTCCTACCGC 
GCCACGCCAG ATTACGAAAC CGTGATGCGA CAGAAAAACA TGATTCCAGC TGCAGAGCGC 
CAGAGCCACT CCATGAGGAA CCTAAATATC GGAAACTCGT ACGCTTACAG CCGACCAGAT 
CCGCTAGTGT ACAGCCAACC GGAAATCCGA GAGCACGGCG CAGGACAGTA TCCTTTTCAC 
CTTAATTACA GCTTCCACAG TCCATCTCCA TACCCCTATC CCACTGAGAG ACGGCCTGTA 
GTGGGCGCTG TAAGCGTTCC CGAACTCACC AACGTCCAGC TGCAGCAGGC GCAGGAATAT 
CCGCCGCCGA ACATCATCAG GAATCAGGTG TACTGGCCTC CGCCGCCGTA TCCGTACCCT 
CATCCTCGAC CCGCCAACAG CACCCCGGAT CTGTCCCGGC ACCTGTACGT CAGCAGCAGT 
AACCCGGATC TGATAACGCG CCGCGTGCAT CACTCGGTGC AGACCTTTCA GGAGGACAGC 
CTGCCAGTGG CGCACTCACT ACAGGAGGTT AGCGAACCGC TAGTCACAGC ACGCCGTCCA 
CACATGCACA AACGCAACTC AATCGAGATC GCAGGACTGG CCTACGGACT GGAGAACATG 
AGGCTCAAGG AGAGGAAGGT ATCCGCATCA GCAGCTGATA CTCCGCCCCC TGTTGCCGCG 
GCAGTGCCCC ATGCAGCATC CGGCTCCGAC ATCAACGTGG TCCTAGAGGT GTCGAAACCA 
GAAGACGTAG GCATCAAAGA GGATGTTTTC TACGGCCACA AGAAGTCTCT GTCGGATGCC 
ACCATGCTGG TGCACAGCAG CGGAGAGGAG GAGGAGTTTG AGGACGACAG CGGCAGACAC 
ACACCACAGT CTCAGGACGC AGTGAGCGGC CCAGAGCATC CCATGACCTC GCTGGGACGC 
TCGCTCGGAG ATCCTCCTGC TTATCCCTTC AGCCACAGTA TGGAGACGGT GCTAACAAGT 
CCTCCGGCCT ACCAGGCGCA TTCCATCATG CAGAAAGCAG AGGACGTGGG GCCACTGCCG 
CCTCTGAGGG AGCCCGGACT CATGATGCCC TCAGTGTCAG AGGGAGATCT GAGCGGACGC 
TTAAGGAAAA ATAGAGACAT CTCGAAAAGA CCTGTGTCTG ACGTGCCTCC GGGGAGGAGA 
AACATTGATG GGCTGCCACC CGCGGGAATG AAAAAAGGAC GCTCGGAGGT GAAGAAGGTC 
GGTCCGCTGA AGCTGGCAGC TCTGAACGGT TTGACCCTGT CCAGGATTCC TCTGCCGGAT 
GATGGAAAGG ACGACCCGGA AAGCGCCTCT AATGATGAAC GGTGTAAGTT TCTGGAGCAG 
CGTTTGGATC AGGGGATGGT GTTCACGGAG TACGAGCAGG TGCCCAAGAA ACGACCCAAC 
AGTGAGTACA GCATCGCTCA GATGCCCGAG AACAACGACA GAAACCGCTT TCAGGACGTC 
CTGCCCTACG ACGACACACG TGTGGAGCTG GTGCCCACCA AAGAGAACAA CACAGGCTAC 
ATCAACGCCT CGCACATCAG GGTGACGGTC AGAGGTAAAG AGTGGAGTTA TATTGCGTCT 
CAGGGTCCGC TTTCCAGCAC CTGTCAGGAT TTCTGGCAGA TGGTTTGGGA ACAAGGAGTG 
TCCATCATCG CAATGGTTAC AGCAGAGGAG GAAGGAGGCC GTGAGAAGAG TTTCCGCTAC 
TGGCCTCGTC TGGGCTCTCG ACACAACACT GTTACATACG GACGCTTCAA GATCACCACA 
CGCTTCCGCA CAGACTCGGG CTGCTACGCC ACCACCGGCC TGAAGATCAA GCATCTACTG 
ACGGGGCAGG AGAGGACCGT CTGGCACCTG CAGTACACCG ACTGGCCCGA TCACGGCTGC 
CCTGAGGACT TTAAAGGCTT TCTCTCGTAT TTGGAGGAGA TCCAGTCTGT GAGGCGACAC 
ACCAACAGTT CCAGCGACCC AAAGAACACA AACCTGCCGG TGCTGGTGCA CTGCAGCGCG 
GGCGTGGGAC GCACCGGTGT GGTCATCCTG TCCGAGATCA TGATCGCCTG TCTGGAACAC 
AATGAGATGC TGGACGTCCC GACGGTTCTG AACCTGCTCC GGCAGCAGCG AATGATGATG 
GTACAGACAC TCTCACAATA CACCTTCATC TACAAAGTCC TCATTCAGTT CCTGAAAAAC 
TCCAGACTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn21 ( XM_005169788.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169845.1 Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22135
Clone ID Related Accession (Same CDS sequence)	XM_005169788.3 , XM_005169788.4
Accession Version	XM_005169788.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169788.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTTC CGTTCGGATT AAAGCTCAAG AGAACCAGGA GATACACGGT GTCCAGCAAG 
AGCTGCCTGG TGACCCGCAT TCAGCTGCTC AATGGAGAAT ACGTGGAGTT CACGCTTTCG 
GTGGAGAGTA CGGGACAGGA GTGTCTGGAG GCGGTCGCGC AGAGGCTGGA GCTACGCGAG 
ATAACGTACT TCAGCCTGTG GTATTACAAC AAGCAGAACC AGCAGAGATG GATTGATCTG 
GAGAAGCCGC TGAAGAAGCA GCTGGACAAG TACGGACTGG AGCCCACCGT TTACTTTGGG 
GTGGTGTTTT ACGTCTGCAC CGTCACCCAA CTACAACAAG AAATAACCAG ATATCAGTAT 
TATCTGCAGC TGAAGAAAGA TGTTCTGGAG GGAAAGATCC CCTGCTCTAT TGAGCAAGCC 
ATTCATCTGG CCAGCCTGGC TGTGCAAGCT GACCTCGGAG ACTTCAATCG GTATGATTCC 
CAGGAATTCC TTCAAAAAAT CGTGCTTTTT CCGATGCCAT GGATACAAGA CGAGAGGGTT 
GTGGAAGAAG CCACTCAGAA AGTCACCATG CTTTATCAGA ACTACCGAGG TCTTTCTGCA 
CCGGAGGCTG AGCTACTGTA CATGCAGGAG GTGGAGAAGA TTGAGGGCTA CGGCGAGGAG 
AGCATCCAAG CCAAGGACTC TCAGGCCACA GACGTGATCA TCGGCTCCTG TCTGGATGGG 
ATTTTTGTCA AGCACAAAAA TGGGAAAGCT CCTCTCATAT TTCGGTGGAA TGACATTAAT 
AATATGACTC ATAATAAGTC CTTCTTTGCC CTGGAGCTCG CCAACAAAGA AGACACAATC 
CAGTTCCAGA CCGAGGACAT GGAGACGTCC AAATATGTGT GCAGAATGTG GCTGGCGAGG 
CACAAGTTTT ACAAGATTAA CAACAGCAGT CTAGAGCCGT CTTGTGACCC GAACTCCGAG 
GGCCCTCAAA GGTCCATTCT CTCTCTTTCA TTTCCTCGCT TTCCAGCCCT CTCTCGTCTC 
TCTGTGCCTG CTGATAAAGG ACAAACTCAG CCAGCTCCTG TAAACCCAGT GAGGCGACGG 
TCCTCCACAA GAATGTCTCT GCCAAAACCG CAGCCCTACA TGATGCCACC TCAGATGCAC 
TTTAACGGCC ACTATAACGA ACCGTACACG TCTTCACAAG ATAATCTCTA CATCAACAAC 
CAGAATGGTT TCTACTACCA TTCCCAAACC AGCCTGGACC GCTCTCCGCA CGAGTACAAC 
GGACGCATCC GAAACGGCAG CGTTTACAGC GCACAGAGCA CCAGCTCCCT GAACAACCCA 
CAGCACTACC TGCAGCCATC GCCGATGTCC TCCAACCCAA GCATCACAGG AAGTGACCTC 
ATGCGTCCCG ACTACGTGCC CTCCCACCGG CACAGCGCCC TGATTCCACC GTCCTACCGC 
GCCACGCCAG ATTACGAAAC CGTGATGCGA CAGAAAAACA TGATTCCAGC TGCAGAGCGC 
CAGAGCCACT CCATGAGGAA CCTAAATATC GGAAACTCGT ACGCTTACAG CCGACCAGAT 
CCGCTAGTGT ACAGCCAACC GGAAATCCGA GAGCACGGCG CAGGACAGTA TCCTTTTCAC 
CTTAATTACA GCTTCCACAG TCCATCTCCA TACCCCTATC CCACTGAGAG ACGGCCTGTA 
GTGGGCGCTG TAAGCGTTCC CGAACTCACC AACGTCCAGC TGCAGCAGGC GCAGGAATAT 
CCGCCGCCGA ACATCATCAG GAATCAGGTG TACTGGCCTC CGCCGCCGTA TCCGTACCCT 
CATCCTCGAC CCGCCAACAG CACCCCGGAT CTGTCCCGGC ACCTGTACGT CAGCAGCAGT 
AACCCGGATC TGATAACGCG CCGCGTGCAT CACTCGGTGC AGACCTTTCA GGAGGACAGC 
CTGCCAGTGG CGCACTCACT ACAGGAGGTT AGCGAACCGC TAGTCACAGC ACGCCGTCCA 
CACATGCACA AACGCAACTC AATCGAGATC GCAGGACTGG CCTACGGACT GGAGAACATG 
AGGCTCAAGG AGAGGAAGGT ATCCGCATCA GCAGCTGATA CTCCGCCCCC TGTTGCCGCG 
GCAGTGCCCC ATGCAGCATC CGGCTCCGAC ATCAACGTGG TCCTAGAGGT GTCGAAACCA 
GAAGACGTAG GCATCAAAGA GGATGTTTTC TACGGCCACA AGAAGTCTCT GTCGGATGCC 
ACCATGCTGG TGCACAGCAG CGGAGAGGAG GAGGAGTTTG AGGACGACAG CGGCAGACAC 
ACACCACAGT CTCAGGACGC AGTGAGCGGC CCAGAGCATC CCATGACCTC GCTGGGACGC 
TCGCTCGGAG ATCCTCCTGC TTATCCCTTC AGCCACAGTA TGGAGACGGT GCTAACAAGT 
CCTCCGGCCT ACCAGGCGCA TTCCATCATG CAGAAAGCAG AGGACGTGGG GCCACTGCCG 
CCTCTGAGGG AGCCCGGACT CATGATGCCC TCAGTGTCAG AGGGAGATCT GAGCGGACGC 
TTAAGGAAAA ATAGAGACAT CTCGAAAAGA CCTGTGTCTG ACGTGCCTCC GGGGAGGAGA 
AACATTGATG GGCTGCCACC CGCGGGAATG AAAAAAGGAC GCTCGGAGGT GAAGAAGGTC 
GGTCCGCTGA AGCTGGCAGC TCTGAACGGT TTGACCCTGT CCAGGATTCC TCTGCCGGAT 
GATGGAAAGG ACGACCCGGA AAGCGCCTCT AATGATGAAC GGTGTAAGTT TCTGGAGCAG 
CGTTTGGATC AGGGGATGGT GTTCACGGAG TACGAGCAGG TGCCCAAGAA ACGACCCAAC 
AGTGAGTACA GCATCGCTCA GATGCCCGAG AACAACGACA GAAACCGCTT TCAGGACGTC 
CTGCCCTACG ACGACACACG TGTGGAGCTG GTGCCCACCA AAGAGAACAA CACAGGCTAC 
ATCAACGCCT CGCACATCAG GGTGACGGTC AGAGGTAAAG AGTGGAGTTA TATTGCGTCT 
CAGGGTCCGC TTTCCAGCAC CTGTCAGGAT TTCTGGCAGA TGGTTTGGGA ACAAGGAGTG 
TCCATCATCG CAATGGTTAC AGCAGAGGAG GAAGGAGGCC GTGAGAAGAG TTTCCGCTAC 
TGGCCTCGTC TGGGCTCTCG ACACAACACT GTTACATACG GACGCTTCAA GATCACCACA 
CGCTTCCGCA CAGACTCGGG CTGCTACGCC ACCACCGGCC TGAAGATCAA GCATCTACTG 
ACGGGGCAGG AGAGGACCGT CTGGCACCTG CAGTACACCG ACTGGCCCGA TCACGGCTGC 
CCTGAGGACT TTAAAGGCTT TCTCTCGTAT TTGGAGGAGA TCCAGTCTGT GAGGCGACAC 
ACCAACAGTT CCAGCGACCC AAAGAACACA AACCTGCCGG TGCTGGTGCA CTGCAGCGCG 
GGCGTGGGAC GCACCGGTGT GGTCATCCTG TCCGAGATCA TGATCGCCTG TCTGGAACAC 
AATGAGATGC TGGACGTCCC GACGGTTCTG AACCTGCTCC GGCAGCAGCG AATGATGATG 
GTACAGACAC TCTCACAATA CACCTTCATC TACAAAGTCC TCATTCAGTT CCTGAAAAAC 
TCCAGACTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169845.1
CDS	301..3915
Translation	MPLPFGLKLKRTRRYTVSSKSCLVTRIQLLNGEYVEFTLSVESTGQECLEAVAQRLELREITYFSLWYYNKQNQQRWIDLEKPLKKQLDKYGLEPTVYFGVVFYVCTVTQLQQEITRYQYYLQLKKDVLEGKIPCSIEQAIHLASLAVQADLGDFNRYDSQEFLQKIVLFPMPWIQDERVVEEATQKVTMLYQNYRGLSAPEAELLYMQEVEKIEGYGEESIQAKDSQATDVIIGSCLDGIFVKHKNGKAPLIFRWNDINNMTHNKSFFALELANKEDTIQFQTEDMETSKYVCRMWLARHKFYKINNSSLEPSCDPNSEGPQRSILSLSFPRFPALSRLSVPADKGQTQPAPVNPVRRRSSTRMSLPKPQPYMMPPQMHFNGHYNEPYTSSQDNLYINNQNGFYYHSQTSLDRSPHEYNGRIRNGSVYSAQSTSSLNNPQHYLQPSPMSSNPSITGSDLMRPDYVPSHRHSALIPPSYRATPDYETVMRQKNMIPAAERQSHSMRNLNIGNSYAYSRPDPLVYSQPEIREHGAGQYPFHLNYSFHSPSPYPYPTERRPVVGAVSVPELTNVQLQQAQEYPPPNIIRNQVYWPPPPYPYPHPRPANSTPDLSRHLYVSSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLVTARRPHMHKRNSIEIAGLAYGLENMRLKERKVSASAADTPPPVAAAVPHAASGSDINVVLEVSKPEDVGIKEDVFYGHKKSLSDATMLVHSSGEEEEFEDDSGRHTPQSQDAVSGPEHPMTSLGRSLGDPPAYPFSHSMETVLTSPPAYQAHSIMQKAEDVGPLPPLREPGLMMPSVSEGDLSGRLRKNRDISKRPVSDVPPGRRNIDGLPPAGMKKGRSEVKKVGPLKLAALNGLTLSRIPLPDDGKDDPESASNDERCKFLEQRLDQGMVFTEYEQVPKKRPNSEYSIAQMPENNDRNRFQDVLPYDDTRVELVPTKENNTGYINASHIRVTVRGKEWSYIASQGPLSSTCQDFWQMVWEQGVSIIAMVTAEEEGGREKSFRYWPRLGSRHNTVTYGRFKITTRFRTDSGCYATTGLKIKHLLTGQERTVWHLQYTDWPDHGCPEDFKGFLSYLEEIQSVRRHTNSSSDPKNTNLPVLVHCSAGVGRTGVVILSEIMIACLEHNEMLDVPTVLNLLRQQRMMMVQTLSQYTFIYKVLIQFLKNSRLI

Target ORF information:

RefSeq Version	XM_005169788.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169788.4

ORF Insert Sequence:

ATGCCCCTTC CGTTCGGATT AAAGCTCAAG AGAACCAGGA GATACACGGT GTCCAGCAAG 
AGCTGCCTGG TGACCCGCAT TCAGCTGCTC AATGGAGAAT ACGTGGAGTT CACGCTTTCG 
GTGGAGAGTA CGGGACAGGA GTGTCTGGAG GCGGTCGCGC AGAGGCTGGA GCTACGCGAG 
ATAACGTACT TCAGCCTGTG GTATTACAAC AAGCAGAACC AGCAGAGATG GATTGATCTG 
GAGAAGCCGC TGAAGAAGCA GCTGGACAAG TACGGACTGG AGCCCACCGT TTACTTTGGG 
GTGGTGTTTT ACGTCTGCAC CGTCACCCAA CTACAACAAG AAATAACCAG ATATCAGTAT 
TATCTGCAGC TGAAGAAAGA TGTTCTGGAG GGAAAGATCC CCTGCTCTAT TGAGCAAGCC 
ATTCATCTGG CCAGCCTGGC TGTGCAAGCT GACCTCGGAG ACTTCAATCG GTATGATTCC 
CAGGAATTCC TTCAAAAAAT CGTGCTTTTT CCGATGCCAT GGATACAAGA CGAGAGGGTT 
GTGGAAGAAG CCACTCAGAA AGTCACCATG CTTTATCAGA ACTACCGAGG TCTTTCTGCA 
CCGGAGGCTG AGCTACTGTA CATGCAGGAG GTGGAGAAGA TTGAGGGCTA CGGCGAGGAG 
AGCATCCAAG CCAAGGACTC TCAGGCCACA GACGTGATCA TCGGCTCCTG TCTGGATGGG 
ATTTTTGTCA AGCACAAAAA TGGGAAAGCT CCTCTCATAT TTCGGTGGAA TGACATTAAT 
AATATGACTC ATAATAAGTC CTTCTTTGCC CTGGAGCTCG CCAACAAAGA AGACACAATC 
CAGTTCCAGA CCGAGGACAT GGAGACGTCC AAATATGTGT GCAGAATGTG GCTGGCGAGG 
CACAAGTTTT ACAAGATTAA CAACAGCAGT CTAGAGCCGT CTTGTGACCC GAACTCCGAG 
GGCCCTCAAA GGTCCATTCT CTCTCTTTCA TTTCCTCGCT TTCCAGCCCT CTCTCGTCTC 
TCTGTGCCTG CTGATAAAGG ACAAACTCAG CCAGCTCCTG TAAACCCAGT GAGGCGACGG 
TCCTCCACAA GAATGTCTCT GCCAAAACCG CAGCCCTACA TGATGCCACC TCAGATGCAC 
TTTAACGGCC ACTATAACGA ACCGTACACG TCTTCACAAG ATAATCTCTA CATCAACAAC 
CAGAATGGTT TCTACTACCA TTCCCAAACC AGCCTGGACC GCTCTCCGCA CGAGTACAAC 
GGACGCATCC GAAACGGCAG CGTTTACAGC GCACAGAGCA CCAGCTCCCT GAACAACCCA 
CAGCACTACC TGCAGCCATC GCCGATGTCC TCCAACCCAA GCATCACAGG AAGTGACCTC 
ATGCGTCCCG ACTACGTGCC CTCCCACCGG CACAGCGCCC TGATTCCACC GTCCTACCGC 
GCCACGCCAG ATTACGAAAC CGTGATGCGA CAGAAAAACA TGATTCCAGC TGCAGAGCGC 
CAGAGCCACT CCATGAGGAA CCTAAATATC GGAAACTCGT ACGCTTACAG CCGACCAGAT 
CCGCTAGTGT ACAGCCAACC GGAAATCCGA GAGCACGGCG CAGGACAGTA TCCTTTTCAC 
CTTAATTACA GCTTCCACAG TCCATCTCCA TACCCCTATC CCACTGAGAG ACGGCCTGTA 
GTGGGCGCTG TAAGCGTTCC CGAACTCACC AACGTCCAGC TGCAGCAGGC GCAGGAATAT 
CCGCCGCCGA ACATCATCAG GAATCAGGTG TACTGGCCTC CGCCGCCGTA TCCGTACCCT 
CATCCTCGAC CCGCCAACAG CACCCCGGAT CTGTCCCGGC ACCTGTACGT CAGCAGCAGT 
AACCCGGATC TGATAACGCG CCGCGTGCAT CACTCGGTGC AGACCTTTCA GGAGGACAGC 
CTGCCAGTGG CGCACTCACT ACAGGAGGTT AGCGAACCGC TAGTCACAGC ACGCCGTCCA 
CACATGCACA AACGCAACTC AATCGAGATC GCAGGACTGG CCTACGGACT GGAGAACATG 
AGGCTCAAGG AGAGGAAGGT ATCCGCATCA GCAGCTGATA CTCCGCCCCC TGTTGCCGCG 
GCAGTGCCCC ATGCAGCATC CGGCTCCGAC ATCAACGTGG TCCTAGAGGT GTCGAAACCA 
GAAGACGTAG GCATCAAAGA GGATGTTTTC TACGGCCACA AGAAGTCTCT GTCGGATGCC 
ACCATGCTGG TGCACAGCAG CGGAGAGGAG GAGGAGTTTG AGGACGACAG CGGCAGACAC 
ACACCACAGT CTCAGGACGC AGTGAGCGGC CCAGAGCATC CCATGACCTC GCTGGGACGC 
TCGCTCGGAG ATCCTCCTGC TTATCCCTTC AGCCACAGTA TGGAGACGGT GCTAACAAGT 
CCTCCGGCCT ACCAGGCGCA TTCCATCATG CAGAAAGCAG AGGACGTGGG GCCACTGCCG 
CCTCTGAGGG AGCCCGGACT CATGATGCCC TCAGTGTCAG AGGGAGATCT GAGCGGACGC 
TTAAGGAAAA ATAGAGACAT CTCGAAAAGA CCTGTGTCTG ACGTGCCTCC GGGGAGGAGA 
AACATTGATG GGCTGCCACC CGCGGGAATG AAAAAAGGAC GCTCGGAGGT GAAGAAGGTC 
GGTCCGCTGA AGCTGGCAGC TCTGAACGGT TTGACCCTGT CCAGGATTCC TCTGCCGGAT 
GATGGAAAGG ACGACCCGGA AAGCGCCTCT AATGATGAAC GGTGTAAGTT TCTGGAGCAG 
CGTTTGGATC AGGGGATGGT GTTCACGGAG TACGAGCAGG TGCCCAAGAA ACGACCCAAC 
AGTGAGTACA GCATCGCTCA GATGCCCGAG AACAACGACA GAAACCGCTT TCAGGACGTC 
CTGCCCTACG ACGACACACG TGTGGAGCTG GTGCCCACCA AAGAGAACAA CACAGGCTAC 
ATCAACGCCT CGCACATCAG GGTGACGGTC AGAGGTAAAG AGTGGAGTTA TATTGCGTCT 
CAGGGTCCGC TTTCCAGCAC CTGTCAGGAT TTCTGGCAGA TGGTTTGGGA ACAAGGAGTG 
TCCATCATCG CAATGGTTAC AGCAGAGGAG GAAGGAGGCC GTGAGAAGAG TTTCCGCTAC 
TGGCCTCGTC TGGGCTCTCG ACACAACACT GTTACATACG GACGCTTCAA GATCACCACA 
CGCTTCCGCA CAGACTCGGG CTGCTACGCC ACCACCGGCC TGAAGATCAA GCATCTACTG 
ACGGGGCAGG AGAGGACCGT CTGGCACCTG CAGTACACCG ACTGGCCCGA TCACGGCTGC 
CCTGAGGACT TTAAAGGCTT TCTCTCGTAT TTGGAGGAGA TCCAGTCTGT GAGGCGACAC 
ACCAACAGTT CCAGCGACCC AAAGAACACA AACCTGCCGG TGCTGGTGCA CTGCAGCGCG 
GGCGTGGGAC GCACCGGTGT GGTCATCCTG TCCGAGATCA TGATCGCCTG TCTGGAACAC 
AATGAGATGC TGGACGTCCC GACGGTTCTG AACCTGCTCC GGCAGCAGCG AATGATGATG 
GTACAGACAC TCTCACAATA CACCTTCATC TACAAAGTCC TCATTCAGTT CCTGAAAAAC 
TCCAGACTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn21 ( XM_001339926.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001339962.4 Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X2, mRNA.

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CloneID	ODa22136
Clone ID Related Accession (Same CDS sequence)	XM_001339926.7 , XM_001339926.8
Accession Version	XM_001339926.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 21 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001339926.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTTC CGTTCGGATT AAAGCTCAAG AGAACCAGGA GATACACGGT GTCCAGCAAG 
AGCTGCCTGG TGACCCGCAT TCAGCTGCTC AATGGAGAAT ACGTGGAGTT CACGCTTTCG 
GTGGAGAGTA CGGGACAGGA GTGTCTGGAG GCGGTCGCGC AGAGGCTGGA GCTACGCGAG 
ATAACGTACT TCAGCCTGTG GTATTACAAC AAGCAGAACC AGCAGAGATG GATTGATCTG 
GAGAAGCCGC TGAAGAAGCA GCTGGACAAG TACGGACTGG AGCCCACCGT TTACTTTGGG 
GTGGTGTTTT ACGTCTGCAC CGTCACCCAA CTACAACAAG AAATAACCAG ATATCAGTAT 
TATCTGCAGC TGAAGAAAGA TGTTCTGGAG GGAAAGATCC CCTGCTCTAT TGAGCAAGCC 
ATTCATCTGG CCAGCCTGGC TGTGCAAGCT GACCTCGGAG ACTTCAATCG GTATGATTCC 
CAGGAATTCC TTCAAAAAAT CGTGCTTTTT CCGATGCCAT GGATACAAGA CGAGAGGGTT 
GTGGAAGAAG CCACTCAGAA AGTCACCATG CTTTATCAGA ACTACCGAGG TCTTTCTGCA 
CCGGAGGCTG AGCTACTGTA CATGCAGGAG GTGGAGAAGA TTGAGGGCTA CGGCGAGGAG 
AGCATCCAAG CCAAGGACTC TCAGGCCACA GACGTGATCA TCGGCTCCTG TCTGGATGGG 
ATTTTTGTCA AGCACAAAAA TGGGAAAGCT CCTCTCATAT TTCGGTGGAA TGACATTAAT 
AATATGACTC ATAATAAGTC CTTCTTTGCC CTGGAGCTCG CCAACAAAGA AGACACAATC 
CAGTTCCAGA CCGAGGACAT GGAGACGTCC AAATATGTGT GCAGAATGTG GCTGGCGAGG 
CACAAGTTTT ACAAGATTAA CAACAGCAGT CTACAAACTC AGCCAGCTCC TGTAAACCCA 
GTGAGGCGAC GGTCCTCCAC AAGAATGTCT CTGCCAAAAC CGCAGCCCTA CATGATGCCA 
CCTCAGATGC ACTTTAACGG CCACTATAAC GAACCGTACA CGTCTTCACA AGATAATCTC 
TACATCAACA ACCAGAATGG TTTCTACTAC CATTCCCAAA CCAGCCTGGA CCGCTCTCCG 
CACGAGTACA ACGGACGCAT CCGAAACGGC AGCGTTTACA GCGCACAGAG CACCAGCTCC 
CTGAACAACC CACAGCACTA CCTGCAGCCA TCGCCGATGT CCTCCAACCC AAGCATCACA 
GGAAGTGACC TCATGCGTCC CGACTACGTG CCCTCCCACC GGCACAGCGC CCTGATTCCA 
CCGTCCTACC GCGCCACGCC AGATTACGAA ACCGTGATGC GACAGAAAAA CATGATTCCA 
GCTGCAGAGC GCCAGAGCCA CTCCATGAGG AACCTAAATA TCGGAAACTC GTACGCTTAC 
AGCCGACCAG ATCCGCTAGT GTACAGCCAA CCGGAAATCC GAGAGCACGG CGCAGGACAG 
TATCCTTTTC ACCTTAATTA CAGCTTCCAC AGTCCATCTC CATACCCCTA TCCCACTGAG 
AGACGGCCTG TAGTGGGCGC TGTAAGCGTT CCCGAACTCA CCAACGTCCA GCTGCAGCAG 
GCGCAGGAAT ATCCGCCGCC GAACATCATC AGGAATCAGG TGTACTGGCC TCCGCCGCCG 
TATCCGTACC CTCATCCTCG ACCCGCCAAC AGCACCCCGG ATCTGTCCCG GCACCTGTAC 
GTCAGCAGCA GTAACCCGGA TCTGATAACG CGCCGCGTGC ATCACTCGGT GCAGACCTTT 
CAGGAGGACA GCCTGCCAGT GGCGCACTCA CTACAGGAGG TTAGCGAACC GCTAGTCACA 
GCACGCCGTC CACACATGCA CAAACGCAAC TCAATCGAGA TCGCAGGACT GGCCTACGGA 
CTGGAGAACA TGAGGCTCAA GGAGAGGAAG GTATCCGCAT CAGCAGCTGA TACTCCGCCC 
CCTGTTGCCG CGGCAGTGCC CCATGCAGCA TCCGGCTCCG ACATCAACGT GGTCCTAGAG 
GTGTCGAAAC CAGAAGACGT AGGCATCAAA GAGGATGTTT TCTACGGCCA CAAGAAGTCT 
CTGTCGGATG CCACCATGCT GGTGCACAGC AGCGGAGAGG AGGAGGAGTT TGAGGACGAC 
AGCGGCAGAC ACACACCACA GTCTCAGGAC GCAGTGAGCG GCCCAGAGCA TCCCATGACC 
TCGCTGGGAC GCTCGCTCGG AGATCCTCCT GCTTATCCCT TCAGCCACAG TATGGAGACG 
GTGCTAACAA GTCCTCCGGC CTACCAGGCG CATTCCATCA TGCAGAAAGC AGAGGACGTG 
GGGCCACTGC CGCCTCTGAG GGAGCCCGGA CTCATGATGC CCTCAGTGTC AGAGGGAGAT 
CTGAGCGGAC GCTTAAGGAA AAATAGAGAC ATCTCGAAAA GACCTGTGTC TGACGTGCCT 
CCGGGGAGGA GAAACATTGA TGGGCTGCCA CCCGCGGGAA TGAAAAAAGG ACGCTCGGAG 
GTGAAGAAGG TCGGTCCGCT GAAGCTGGCA GCTCTGAACG GTTTGACCCT GTCCAGGATT 
CCTCTGCCGG ATGATGGAAA GGACGACCCG GAAAGCGCCT CTAATGATGA ACGGTGTAAG 
TTTCTGGAGC AGCGTTTGGA TCAGGGGATG GTGTTCACGG AGTACGAGCA GGTGCCCAAG 
AAACGACCCA ACAGTGAGTA CAGCATCGCT CAGATGCCCG AGAACAACGA CAGAAACCGC 
TTTCAGGACG TCCTGCCCTA CGACGACACA CGTGTGGAGC TGGTGCCCAC CAAAGAGAAC 
AACACAGGCT ACATCAACGC CTCGCACATC AGGGTGACGG TCAGAGGTAA AGAGTGGAGT 
TATATTGCGT CTCAGGGTCC GCTTTCCAGC ACCTGTCAGG ATTTCTGGCA GATGGTTTGG 
GAACAAGGAG TGTCCATCAT CGCAATGGTT ACAGCAGAGG AGGAAGGAGG CCGTGAGAAG 
AGTTTCCGCT ACTGGCCTCG TCTGGGCTCT CGACACAACA CTGTTACATA CGGACGCTTC 
AAGATCACCA CACGCTTCCG CACAGACTCG GGCTGCTACG CCACCACCGG CCTGAAGATC 
AAGCATCTAC TGACGGGGCA GGAGAGGACC GTCTGGCACC TGCAGTACAC CGACTGGCCC 
GATCACGGCT GCCCTGAGGA CTTTAAAGGC TTTCTCTCGT ATTTGGAGGA GATCCAGTCT 
GTGAGGCGAC ACACCAACAG TTCCAGCGAC CCAAAGAACA CAAACCTGCC GGTGCTGGTG 
CACTGCAGCG CGGGCGTGGG ACGCACCGGT GTGGTCATCC TGTCCGAGAT CATGATCGCC 
TGTCTGGAAC ACAATGAGAT GCTGGACGTC CCGACGGTTC TGAACCTGCT CCGGCAGCAG 
CGAATGATGA TGGTACAGAC ACTCTCACAA TACACCTTCA TCTACAAAGT CCTCATTCAG 
TTCCTGAAAA ACTCCAGACT GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001339962.4
CDS	301..3807
Translation	MPLPFGLKLKRTRRYTVSSKSCLVTRIQLLNGEYVEFTLSVESTGQECLEAVAQRLELREITYFSLWYYNKQNQQRWIDLEKPLKKQLDKYGLEPTVYFGVVFYVCTVTQLQQEITRYQYYLQLKKDVLEGKIPCSIEQAIHLASLAVQADLGDFNRYDSQEFLQKIVLFPMPWIQDERVVEEATQKVTMLYQNYRGLSAPEAELLYMQEVEKIEGYGEESIQAKDSQATDVIIGSCLDGIFVKHKNGKAPLIFRWNDINNMTHNKSFFALELANKEDTIQFQTEDMETSKYVCRMWLARHKFYKINNSSLQTQPAPVNPVRRRSSTRMSLPKPQPYMMPPQMHFNGHYNEPYTSSQDNLYINNQNGFYYHSQTSLDRSPHEYNGRIRNGSVYSAQSTSSLNNPQHYLQPSPMSSNPSITGSDLMRPDYVPSHRHSALIPPSYRATPDYETVMRQKNMIPAAERQSHSMRNLNIGNSYAYSRPDPLVYSQPEIREHGAGQYPFHLNYSFHSPSPYPYPTERRPVVGAVSVPELTNVQLQQAQEYPPPNIIRNQVYWPPPPYPYPHPRPANSTPDLSRHLYVSSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLVTARRPHMHKRNSIEIAGLAYGLENMRLKERKVSASAADTPPPVAAAVPHAASGSDINVVLEVSKPEDVGIKEDVFYGHKKSLSDATMLVHSSGEEEEFEDDSGRHTPQSQDAVSGPEHPMTSLGRSLGDPPAYPFSHSMETVLTSPPAYQAHSIMQKAEDVGPLPPLREPGLMMPSVSEGDLSGRLRKNRDISKRPVSDVPPGRRNIDGLPPAGMKKGRSEVKKVGPLKLAALNGLTLSRIPLPDDGKDDPESASNDERCKFLEQRLDQGMVFTEYEQVPKKRPNSEYSIAQMPENNDRNRFQDVLPYDDTRVELVPTKENNTGYINASHIRVTVRGKEWSYIASQGPLSSTCQDFWQMVWEQGVSIIAMVTAEEEGGREKSFRYWPRLGSRHNTVTYGRFKITTRFRTDSGCYATTGLKIKHLLTGQERTVWHLQYTDWPDHGCPEDFKGFLSYLEEIQSVRRHTNSSSDPKNTNLPVLVHCSAGVGRTGVVILSEIMIACLEHNEMLDVPTVLNLLRQQRMMMVQTLSQYTFIYKVLIQFLKNSRLI

Target ORF information:

RefSeq Version	XM_001339926.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001339926.7

ORF Insert Sequence:

ATGCCCCTTC CGTTCGGATT AAAGCTCAAG AGAACCAGGA GATACACGGT GTCCAGCAAG 
AGCTGCCTGG TGACCCGCAT TCAGCTGCTC AATGGAGAAT ACGTGGAGTT CACGCTTTCG 
GTGGAGAGTA CGGGACAGGA GTGTCTGGAG GCGGTCGCGC AGAGGCTGGA GCTACGCGAG 
ATAACGTACT TCAGCCTGTG GTATTACAAC AAGCAGAACC AGCAGAGATG GATTGATCTG 
GAGAAGCCGC TGAAGAAGCA GCTGGACAAG TACGGACTGG AGCCCACCGT TTACTTTGGG 
GTGGTGTTTT ACGTCTGCAC CGTCACCCAA CTACAACAAG AAATAACCAG ATATCAGTAT 
TATCTGCAGC TGAAGAAAGA TGTTCTGGAG GGAAAGATCC CCTGCTCTAT TGAGCAAGCC 
ATTCATCTGG CCAGCCTGGC TGTGCAAGCT GACCTCGGAG ACTTCAATCG GTATGATTCC 
CAGGAATTCC TTCAAAAAAT CGTGCTTTTT CCGATGCCAT GGATACAAGA CGAGAGGGTT 
GTGGAAGAAG CCACTCAGAA AGTCACCATG CTTTATCAGA ACTACCGAGG TCTTTCTGCA 
CCGGAGGCTG AGCTACTGTA CATGCAGGAG GTGGAGAAGA TTGAGGGCTA CGGCGAGGAG 
AGCATCCAAG CCAAGGACTC TCAGGCCACA GACGTGATCA TCGGCTCCTG TCTGGATGGG 
ATTTTTGTCA AGCACAAAAA TGGGAAAGCT CCTCTCATAT TTCGGTGGAA TGACATTAAT 
AATATGACTC ATAATAAGTC CTTCTTTGCC CTGGAGCTCG CCAACAAAGA AGACACAATC 
CAGTTCCAGA CCGAGGACAT GGAGACGTCC AAATATGTGT GCAGAATGTG GCTGGCGAGG 
CACAAGTTTT ACAAGATTAA CAACAGCAGT CTACAAACTC AGCCAGCTCC TGTAAACCCA 
GTGAGGCGAC GGTCCTCCAC AAGAATGTCT CTGCCAAAAC CGCAGCCCTA CATGATGCCA 
CCTCAGATGC ACTTTAACGG CCACTATAAC GAACCGTACA CGTCTTCACA AGATAATCTC 
TACATCAACA ACCAGAATGG TTTCTACTAC CATTCCCAAA CCAGCCTGGA CCGCTCTCCG 
CACGAGTACA ACGGACGCAT CCGAAACGGC AGCGTTTACA GCGCACAGAG CACCAGCTCC 
CTGAACAACC CACAGCACTA CCTGCAGCCA TCGCCGATGT CCTCCAACCC AAGCATCACA 
GGAAGTGACC TCATGCGTCC CGACTACGTG CCCTCCCACC GGCACAGCGC CCTGATTCCA 
CCGTCCTACC GCGCCACGCC AGATTACGAA ACCGTGATGC GACAGAAAAA CATGATTCCA 
GCTGCAGAGC GCCAGAGCCA CTCCATGAGG AACCTAAATA TCGGAAACTC GTACGCTTAC 
AGCCGACCAG ATCCGCTAGT GTACAGCCAA CCGGAAATCC GAGAGCACGG CGCAGGACAG 
TATCCTTTTC ACCTTAATTA CAGCTTCCAC AGTCCATCTC CATACCCCTA TCCCACTGAG 
AGACGGCCTG TAGTGGGCGC TGTAAGCGTT CCCGAACTCA CCAACGTCCA GCTGCAGCAG 
GCGCAGGAAT ATCCGCCGCC GAACATCATC AGGAATCAGG TGTACTGGCC TCCGCCGCCG 
TATCCGTACC CTCATCCTCG ACCCGCCAAC AGCACCCCGG ATCTGTCCCG GCACCTGTAC 
GTCAGCAGCA GTAACCCGGA TCTGATAACG CGCCGCGTGC ATCACTCGGT GCAGACCTTT 
CAGGAGGACA GCCTGCCAGT GGCGCACTCA CTACAGGAGG TTAGCGAACC GCTAGTCACA 
GCACGCCGTC CACACATGCA CAAACGCAAC TCAATCGAGA TCGCAGGACT GGCCTACGGA 
CTGGAGAACA TGAGGCTCAA GGAGAGGAAG GTATCCGCAT CAGCAGCTGA TACTCCGCCC 
CCTGTTGCCG CGGCAGTGCC CCATGCAGCA TCCGGCTCCG ACATCAACGT GGTCCTAGAG 
GTGTCGAAAC CAGAAGACGT AGGCATCAAA GAGGATGTTT TCTACGGCCA CAAGAAGTCT 
CTGTCGGATG CCACCATGCT GGTGCACAGC AGCGGAGAGG AGGAGGAGTT TGAGGACGAC 
AGCGGCAGAC ACACACCACA GTCTCAGGAC GCAGTGAGCG GCCCAGAGCA TCCCATGACC 
TCGCTGGGAC GCTCGCTCGG AGATCCTCCT GCTTATCCCT TCAGCCACAG TATGGAGACG 
GTGCTAACAA GTCCTCCGGC CTACCAGGCG CATTCCATCA TGCAGAAAGC AGAGGACGTG 
GGGCCACTGC CGCCTCTGAG GGAGCCCGGA CTCATGATGC CCTCAGTGTC AGAGGGAGAT 
CTGAGCGGAC GCTTAAGGAA AAATAGAGAC ATCTCGAAAA GACCTGTGTC TGACGTGCCT 
CCGGGGAGGA GAAACATTGA TGGGCTGCCA CCCGCGGGAA TGAAAAAAGG ACGCTCGGAG 
GTGAAGAAGG TCGGTCCGCT GAAGCTGGCA GCTCTGAACG GTTTGACCCT GTCCAGGATT 
CCTCTGCCGG ATGATGGAAA GGACGACCCG GAAAGCGCCT CTAATGATGA ACGGTGTAAG 
TTTCTGGAGC AGCGTTTGGA TCAGGGGATG GTGTTCACGG AGTACGAGCA GGTGCCCAAG 
AAACGACCCA ACAGTGAGTA CAGCATCGCT CAGATGCCCG AGAACAACGA CAGAAACCGC 
TTTCAGGACG TCCTGCCCTA CGACGACACA CGTGTGGAGC TGGTGCCCAC CAAAGAGAAC 
AACACAGGCT ACATCAACGC CTCGCACATC AGGGTGACGG TCAGAGGTAA AGAGTGGAGT 
TATATTGCGT CTCAGGGTCC GCTTTCCAGC ACCTGTCAGG ATTTCTGGCA GATGGTTTGG 
GAACAAGGAG TGTCCATCAT CGCAATGGTT ACAGCAGAGG AGGAAGGAGG CCGTGAGAAG 
AGTTTCCGCT ACTGGCCTCG TCTGGGCTCT CGACACAACA CTGTTACATA CGGACGCTTC 
AAGATCACCA CACGCTTCCG CACAGACTCG GGCTGCTACG CCACCACCGG CCTGAAGATC 
AAGCATCTAC TGACGGGGCA GGAGAGGACC GTCTGGCACC TGCAGTACAC CGACTGGCCC 
GATCACGGCT GCCCTGAGGA CTTTAAAGGC TTTCTCTCGT ATTTGGAGGA GATCCAGTCT 
GTGAGGCGAC ACACCAACAG TTCCAGCGAC CCAAAGAACA CAAACCTGCC GGTGCTGGTG 
CACTGCAGCG CGGGCGTGGG ACGCACCGGT GTGGTCATCC TGTCCGAGAT CATGATCGCC 
TGTCTGGAAC ACAATGAGAT GCTGGACGTC CCGACGGTTC TGAACCTGCT CCGGCAGCAG 
CGAATGATGA TGGTACAGAC ACTCTCACAA TACACCTTCA TCTACAAAGT CCTCATTCAG 
TTCCTGAAAA ACTCCAGACT GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn21 ( XM_001339926.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001339962.4 Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X2, mRNA.

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CloneID	ODa22136
Clone ID Related Accession (Same CDS sequence)	XM_001339926.7 , XM_001339926.8
Accession Version	XM_001339926.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001339926.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTTC CGTTCGGATT AAAGCTCAAG AGAACCAGGA GATACACGGT GTCCAGCAAG 
AGCTGCCTGG TGACCCGCAT TCAGCTGCTC AATGGAGAAT ACGTGGAGTT CACGCTTTCG 
GTGGAGAGTA CGGGACAGGA GTGTCTGGAG GCGGTCGCGC AGAGGCTGGA GCTACGCGAG 
ATAACGTACT TCAGCCTGTG GTATTACAAC AAGCAGAACC AGCAGAGATG GATTGATCTG 
GAGAAGCCGC TGAAGAAGCA GCTGGACAAG TACGGACTGG AGCCCACCGT TTACTTTGGG 
GTGGTGTTTT ACGTCTGCAC CGTCACCCAA CTACAACAAG AAATAACCAG ATATCAGTAT 
TATCTGCAGC TGAAGAAAGA TGTTCTGGAG GGAAAGATCC CCTGCTCTAT TGAGCAAGCC 
ATTCATCTGG CCAGCCTGGC TGTGCAAGCT GACCTCGGAG ACTTCAATCG GTATGATTCC 
CAGGAATTCC TTCAAAAAAT CGTGCTTTTT CCGATGCCAT GGATACAAGA CGAGAGGGTT 
GTGGAAGAAG CCACTCAGAA AGTCACCATG CTTTATCAGA ACTACCGAGG TCTTTCTGCA 
CCGGAGGCTG AGCTACTGTA CATGCAGGAG GTGGAGAAGA TTGAGGGCTA CGGCGAGGAG 
AGCATCCAAG CCAAGGACTC TCAGGCCACA GACGTGATCA TCGGCTCCTG TCTGGATGGG 
ATTTTTGTCA AGCACAAAAA TGGGAAAGCT CCTCTCATAT TTCGGTGGAA TGACATTAAT 
AATATGACTC ATAATAAGTC CTTCTTTGCC CTGGAGCTCG CCAACAAAGA AGACACAATC 
CAGTTCCAGA CCGAGGACAT GGAGACGTCC AAATATGTGT GCAGAATGTG GCTGGCGAGG 
CACAAGTTTT ACAAGATTAA CAACAGCAGT CTACAAACTC AGCCAGCTCC TGTAAACCCA 
GTGAGGCGAC GGTCCTCCAC AAGAATGTCT CTGCCAAAAC CGCAGCCCTA CATGATGCCA 
CCTCAGATGC ACTTTAACGG CCACTATAAC GAACCGTACA CGTCTTCACA AGATAATCTC 
TACATCAACA ACCAGAATGG TTTCTACTAC CATTCCCAAA CCAGCCTGGA CCGCTCTCCG 
CACGAGTACA ACGGACGCAT CCGAAACGGC AGCGTTTACA GCGCACAGAG CACCAGCTCC 
CTGAACAACC CACAGCACTA CCTGCAGCCA TCGCCGATGT CCTCCAACCC AAGCATCACA 
GGAAGTGACC TCATGCGTCC CGACTACGTG CCCTCCCACC GGCACAGCGC CCTGATTCCA 
CCGTCCTACC GCGCCACGCC AGATTACGAA ACCGTGATGC GACAGAAAAA CATGATTCCA 
GCTGCAGAGC GCCAGAGCCA CTCCATGAGG AACCTAAATA TCGGAAACTC GTACGCTTAC 
AGCCGACCAG ATCCGCTAGT GTACAGCCAA CCGGAAATCC GAGAGCACGG CGCAGGACAG 
TATCCTTTTC ACCTTAATTA CAGCTTCCAC AGTCCATCTC CATACCCCTA TCCCACTGAG 
AGACGGCCTG TAGTGGGCGC TGTAAGCGTT CCCGAACTCA CCAACGTCCA GCTGCAGCAG 
GCGCAGGAAT ATCCGCCGCC GAACATCATC AGGAATCAGG TGTACTGGCC TCCGCCGCCG 
TATCCGTACC CTCATCCTCG ACCCGCCAAC AGCACCCCGG ATCTGTCCCG GCACCTGTAC 
GTCAGCAGCA GTAACCCGGA TCTGATAACG CGCCGCGTGC ATCACTCGGT GCAGACCTTT 
CAGGAGGACA GCCTGCCAGT GGCGCACTCA CTACAGGAGG TTAGCGAACC GCTAGTCACA 
GCACGCCGTC CACACATGCA CAAACGCAAC TCAATCGAGA TCGCAGGACT GGCCTACGGA 
CTGGAGAACA TGAGGCTCAA GGAGAGGAAG GTATCCGCAT CAGCAGCTGA TACTCCGCCC 
CCTGTTGCCG CGGCAGTGCC CCATGCAGCA TCCGGCTCCG ACATCAACGT GGTCCTAGAG 
GTGTCGAAAC CAGAAGACGT AGGCATCAAA GAGGATGTTT TCTACGGCCA CAAGAAGTCT 
CTGTCGGATG CCACCATGCT GGTGCACAGC AGCGGAGAGG AGGAGGAGTT TGAGGACGAC 
AGCGGCAGAC ACACACCACA GTCTCAGGAC GCAGTGAGCG GCCCAGAGCA TCCCATGACC 
TCGCTGGGAC GCTCGCTCGG AGATCCTCCT GCTTATCCCT TCAGCCACAG TATGGAGACG 
GTGCTAACAA GTCCTCCGGC CTACCAGGCG CATTCCATCA TGCAGAAAGC AGAGGACGTG 
GGGCCACTGC CGCCTCTGAG GGAGCCCGGA CTCATGATGC CCTCAGTGTC AGAGGGAGAT 
CTGAGCGGAC GCTTAAGGAA AAATAGAGAC ATCTCGAAAA GACCTGTGTC TGACGTGCCT 
CCGGGGAGGA GAAACATTGA TGGGCTGCCA CCCGCGGGAA TGAAAAAAGG ACGCTCGGAG 
GTGAAGAAGG TCGGTCCGCT GAAGCTGGCA GCTCTGAACG GTTTGACCCT GTCCAGGATT 
CCTCTGCCGG ATGATGGAAA GGACGACCCG GAAAGCGCCT CTAATGATGA ACGGTGTAAG 
TTTCTGGAGC AGCGTTTGGA TCAGGGGATG GTGTTCACGG AGTACGAGCA GGTGCCCAAG 
AAACGACCCA ACAGTGAGTA CAGCATCGCT CAGATGCCCG AGAACAACGA CAGAAACCGC 
TTTCAGGACG TCCTGCCCTA CGACGACACA CGTGTGGAGC TGGTGCCCAC CAAAGAGAAC 
AACACAGGCT ACATCAACGC CTCGCACATC AGGGTGACGG TCAGAGGTAA AGAGTGGAGT 
TATATTGCGT CTCAGGGTCC GCTTTCCAGC ACCTGTCAGG ATTTCTGGCA GATGGTTTGG 
GAACAAGGAG TGTCCATCAT CGCAATGGTT ACAGCAGAGG AGGAAGGAGG CCGTGAGAAG 
AGTTTCCGCT ACTGGCCTCG TCTGGGCTCT CGACACAACA CTGTTACATA CGGACGCTTC 
AAGATCACCA CACGCTTCCG CACAGACTCG GGCTGCTACG CCACCACCGG CCTGAAGATC 
AAGCATCTAC TGACGGGGCA GGAGAGGACC GTCTGGCACC TGCAGTACAC CGACTGGCCC 
GATCACGGCT GCCCTGAGGA CTTTAAAGGC TTTCTCTCGT ATTTGGAGGA GATCCAGTCT 
GTGAGGCGAC ACACCAACAG TTCCAGCGAC CCAAAGAACA CAAACCTGCC GGTGCTGGTG 
CACTGCAGCG CGGGCGTGGG ACGCACCGGT GTGGTCATCC TGTCCGAGAT CATGATCGCC 
TGTCTGGAAC ACAATGAGAT GCTGGACGTC CCGACGGTTC TGAACCTGCT CCGGCAGCAG 
CGAATGATGA TGGTACAGAC ACTCTCACAA TACACCTTCA TCTACAAAGT CCTCATTCAG 
TTCCTGAAAA ACTCCAGACT GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001339962.4
CDS	301..3807
Translation	MPLPFGLKLKRTRRYTVSSKSCLVTRIQLLNGEYVEFTLSVESTGQECLEAVAQRLELREITYFSLWYYNKQNQQRWIDLEKPLKKQLDKYGLEPTVYFGVVFYVCTVTQLQQEITRYQYYLQLKKDVLEGKIPCSIEQAIHLASLAVQADLGDFNRYDSQEFLQKIVLFPMPWIQDERVVEEATQKVTMLYQNYRGLSAPEAELLYMQEVEKIEGYGEESIQAKDSQATDVIIGSCLDGIFVKHKNGKAPLIFRWNDINNMTHNKSFFALELANKEDTIQFQTEDMETSKYVCRMWLARHKFYKINNSSLQTQPAPVNPVRRRSSTRMSLPKPQPYMMPPQMHFNGHYNEPYTSSQDNLYINNQNGFYYHSQTSLDRSPHEYNGRIRNGSVYSAQSTSSLNNPQHYLQPSPMSSNPSITGSDLMRPDYVPSHRHSALIPPSYRATPDYETVMRQKNMIPAAERQSHSMRNLNIGNSYAYSRPDPLVYSQPEIREHGAGQYPFHLNYSFHSPSPYPYPTERRPVVGAVSVPELTNVQLQQAQEYPPPNIIRNQVYWPPPPYPYPHPRPANSTPDLSRHLYVSSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLVTARRPHMHKRNSIEIAGLAYGLENMRLKERKVSASAADTPPPVAAAVPHAASGSDINVVLEVSKPEDVGIKEDVFYGHKKSLSDATMLVHSSGEEEEFEDDSGRHTPQSQDAVSGPEHPMTSLGRSLGDPPAYPFSHSMETVLTSPPAYQAHSIMQKAEDVGPLPPLREPGLMMPSVSEGDLSGRLRKNRDISKRPVSDVPPGRRNIDGLPPAGMKKGRSEVKKVGPLKLAALNGLTLSRIPLPDDGKDDPESASNDERCKFLEQRLDQGMVFTEYEQVPKKRPNSEYSIAQMPENNDRNRFQDVLPYDDTRVELVPTKENNTGYINASHIRVTVRGKEWSYIASQGPLSSTCQDFWQMVWEQGVSIIAMVTAEEEGGREKSFRYWPRLGSRHNTVTYGRFKITTRFRTDSGCYATTGLKIKHLLTGQERTVWHLQYTDWPDHGCPEDFKGFLSYLEEIQSVRRHTNSSSDPKNTNLPVLVHCSAGVGRTGVVILSEIMIACLEHNEMLDVPTVLNLLRQQRMMMVQTLSQYTFIYKVLIQFLKNSRLI

Target ORF information:

RefSeq Version	XM_001339926.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 21 (ptpn21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001339926.8

ORF Insert Sequence:

ATGCCCCTTC CGTTCGGATT AAAGCTCAAG AGAACCAGGA GATACACGGT GTCCAGCAAG 
AGCTGCCTGG TGACCCGCAT TCAGCTGCTC AATGGAGAAT ACGTGGAGTT CACGCTTTCG 
GTGGAGAGTA CGGGACAGGA GTGTCTGGAG GCGGTCGCGC AGAGGCTGGA GCTACGCGAG 
ATAACGTACT TCAGCCTGTG GTATTACAAC AAGCAGAACC AGCAGAGATG GATTGATCTG 
GAGAAGCCGC TGAAGAAGCA GCTGGACAAG TACGGACTGG AGCCCACCGT TTACTTTGGG 
GTGGTGTTTT ACGTCTGCAC CGTCACCCAA CTACAACAAG AAATAACCAG ATATCAGTAT 
TATCTGCAGC TGAAGAAAGA TGTTCTGGAG GGAAAGATCC CCTGCTCTAT TGAGCAAGCC 
ATTCATCTGG CCAGCCTGGC TGTGCAAGCT GACCTCGGAG ACTTCAATCG GTATGATTCC 
CAGGAATTCC TTCAAAAAAT CGTGCTTTTT CCGATGCCAT GGATACAAGA CGAGAGGGTT 
GTGGAAGAAG CCACTCAGAA AGTCACCATG CTTTATCAGA ACTACCGAGG TCTTTCTGCA 
CCGGAGGCTG AGCTACTGTA CATGCAGGAG GTGGAGAAGA TTGAGGGCTA CGGCGAGGAG 
AGCATCCAAG CCAAGGACTC TCAGGCCACA GACGTGATCA TCGGCTCCTG TCTGGATGGG 
ATTTTTGTCA AGCACAAAAA TGGGAAAGCT CCTCTCATAT TTCGGTGGAA TGACATTAAT 
AATATGACTC ATAATAAGTC CTTCTTTGCC CTGGAGCTCG CCAACAAAGA AGACACAATC 
CAGTTCCAGA CCGAGGACAT GGAGACGTCC AAATATGTGT GCAGAATGTG GCTGGCGAGG 
CACAAGTTTT ACAAGATTAA CAACAGCAGT CTACAAACTC AGCCAGCTCC TGTAAACCCA 
GTGAGGCGAC GGTCCTCCAC AAGAATGTCT CTGCCAAAAC CGCAGCCCTA CATGATGCCA 
CCTCAGATGC ACTTTAACGG CCACTATAAC GAACCGTACA CGTCTTCACA AGATAATCTC 
TACATCAACA ACCAGAATGG TTTCTACTAC CATTCCCAAA CCAGCCTGGA CCGCTCTCCG 
CACGAGTACA ACGGACGCAT CCGAAACGGC AGCGTTTACA GCGCACAGAG CACCAGCTCC 
CTGAACAACC CACAGCACTA CCTGCAGCCA TCGCCGATGT CCTCCAACCC AAGCATCACA 
GGAAGTGACC TCATGCGTCC CGACTACGTG CCCTCCCACC GGCACAGCGC CCTGATTCCA 
CCGTCCTACC GCGCCACGCC AGATTACGAA ACCGTGATGC GACAGAAAAA CATGATTCCA 
GCTGCAGAGC GCCAGAGCCA CTCCATGAGG AACCTAAATA TCGGAAACTC GTACGCTTAC 
AGCCGACCAG ATCCGCTAGT GTACAGCCAA CCGGAAATCC GAGAGCACGG CGCAGGACAG 
TATCCTTTTC ACCTTAATTA CAGCTTCCAC AGTCCATCTC CATACCCCTA TCCCACTGAG 
AGACGGCCTG TAGTGGGCGC TGTAAGCGTT CCCGAACTCA CCAACGTCCA GCTGCAGCAG 
GCGCAGGAAT ATCCGCCGCC GAACATCATC AGGAATCAGG TGTACTGGCC TCCGCCGCCG 
TATCCGTACC CTCATCCTCG ACCCGCCAAC AGCACCCCGG ATCTGTCCCG GCACCTGTAC 
GTCAGCAGCA GTAACCCGGA TCTGATAACG CGCCGCGTGC ATCACTCGGT GCAGACCTTT 
CAGGAGGACA GCCTGCCAGT GGCGCACTCA CTACAGGAGG TTAGCGAACC GCTAGTCACA 
GCACGCCGTC CACACATGCA CAAACGCAAC TCAATCGAGA TCGCAGGACT GGCCTACGGA 
CTGGAGAACA TGAGGCTCAA GGAGAGGAAG GTATCCGCAT CAGCAGCTGA TACTCCGCCC 
CCTGTTGCCG CGGCAGTGCC CCATGCAGCA TCCGGCTCCG ACATCAACGT GGTCCTAGAG 
GTGTCGAAAC CAGAAGACGT AGGCATCAAA GAGGATGTTT TCTACGGCCA CAAGAAGTCT 
CTGTCGGATG CCACCATGCT GGTGCACAGC AGCGGAGAGG AGGAGGAGTT TGAGGACGAC 
AGCGGCAGAC ACACACCACA GTCTCAGGAC GCAGTGAGCG GCCCAGAGCA TCCCATGACC 
TCGCTGGGAC GCTCGCTCGG AGATCCTCCT GCTTATCCCT TCAGCCACAG TATGGAGACG 
GTGCTAACAA GTCCTCCGGC CTACCAGGCG CATTCCATCA TGCAGAAAGC AGAGGACGTG 
GGGCCACTGC CGCCTCTGAG GGAGCCCGGA CTCATGATGC CCTCAGTGTC AGAGGGAGAT 
CTGAGCGGAC GCTTAAGGAA AAATAGAGAC ATCTCGAAAA GACCTGTGTC TGACGTGCCT 
CCGGGGAGGA GAAACATTGA TGGGCTGCCA CCCGCGGGAA TGAAAAAAGG ACGCTCGGAG 
GTGAAGAAGG TCGGTCCGCT GAAGCTGGCA GCTCTGAACG GTTTGACCCT GTCCAGGATT 
CCTCTGCCGG ATGATGGAAA GGACGACCCG GAAAGCGCCT CTAATGATGA ACGGTGTAAG 
TTTCTGGAGC AGCGTTTGGA TCAGGGGATG GTGTTCACGG AGTACGAGCA GGTGCCCAAG 
AAACGACCCA ACAGTGAGTA CAGCATCGCT CAGATGCCCG AGAACAACGA CAGAAACCGC 
TTTCAGGACG TCCTGCCCTA CGACGACACA CGTGTGGAGC TGGTGCCCAC CAAAGAGAAC 
AACACAGGCT ACATCAACGC CTCGCACATC AGGGTGACGG TCAGAGGTAA AGAGTGGAGT 
TATATTGCGT CTCAGGGTCC GCTTTCCAGC ACCTGTCAGG ATTTCTGGCA GATGGTTTGG 
GAACAAGGAG TGTCCATCAT CGCAATGGTT ACAGCAGAGG AGGAAGGAGG CCGTGAGAAG 
AGTTTCCGCT ACTGGCCTCG TCTGGGCTCT CGACACAACA CTGTTACATA CGGACGCTTC 
AAGATCACCA CACGCTTCCG CACAGACTCG GGCTGCTACG CCACCACCGG CCTGAAGATC 
AAGCATCTAC TGACGGGGCA GGAGAGGACC GTCTGGCACC TGCAGTACAC CGACTGGCCC 
GATCACGGCT GCCCTGAGGA CTTTAAAGGC TTTCTCTCGT ATTTGGAGGA GATCCAGTCT 
GTGAGGCGAC ACACCAACAG TTCCAGCGAC CCAAAGAACA CAAACCTGCC GGTGCTGGTG 
CACTGCAGCG CGGGCGTGGG ACGCACCGGT GTGGTCATCC TGTCCGAGAT CATGATCGCC 
TGTCTGGAAC ACAATGAGAT GCTGGACGTC CCGACGGTTC TGAACCTGCT CCGGCAGCAG 
CGAATGATGA TGGTACAGAC ACTCTCACAA TACACCTTCA TCTACAAAGT CCTCATTCAG 
TTCCTGAAAA ACTCCAGACT GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ptpn21 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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