hwt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hwt
Entrez Gene ID	32202
Full Name	CG43921 gene product from transcript CG43921-RA
Synonyms	43921,BcDNA:GH02424,CG11146,CG15729,CG32653,CG42251,Dmel\CG43921,Dmel_CG11146,Dmel_CG15729,Dmel_CG32653,Dmel_CG42251,lincRNA.981
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11A12-11A12

mRNA and Protein(s)

mRNA	Protein	Name
NM_132578.3	NP_572806.2	heavyweight, isoform C
NM_001272555.2	NP_001259484.1	heavyweight, isoform A
NM_001298227.1	NP_001285156.1	heavyweight, isoform D
NM_167324.3	NP_727617.2	heavyweight, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hwt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hwt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01400	NM_132578.3 Latest version!	Drosophila melanogaster heavyweight (hwt), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01401	NM_167324.3 Latest version!	Drosophila melanogaster heavyweight (hwt), transcript variant B, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa02800	NM_001272555.2 Latest version!	Drosophila melanogaster heavyweight (hwt), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa21443	NM_001298227.1 Latest version!	Drosophila melanogaster heavyweight (hwt), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hwt ( NM_132578.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572806.2 Drosophila melanogaster heavyweight (hwt), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01400
Clone ID Related Accession (Same CDS sequence)	NM_132578.3
Accession Version	NM_132578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heavyweight, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132578.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACCGC ACCGCCTGCT GGGACCAGCA GCCGTGTGCG GAGCCTGCAT CACATCCGCT 
CCTGCCTCCT CCTTGGCCGG CGGACGGGGC AGCGAGCTCG GCGATGATCT GGAAGAGGAG 
TACTTCCGGC CGCGCAGCAT CTTCTATGTG CATCCACATG GCGTCCACGA GTGCATCGAG 
TGCGCCCAGC CAGCCCAGCC TGCGACTGTT CCAGCGCCAA CGCTGAGCAA TCCATCGCTC 
GATATCTACG GCGACGACGA GGACGAAGTG GACGGTGACG AGGACGAGGA CGAAGAGGAC 
GACTCCGAGG ACATCATGGG CAGCCGGCGG CGGCAGATCT ATGAGACGGC CTTCGACTGC 
AAGATAGCCA AGTCGGATGA CGATCTAGAT GAGGTGGATC GCATTACCAA TTGCTCGGTC 
TTGCTGCAGT TGAGTAATGG CAACGGTGGT GACATCAGCA AGGTAACCGG CTCTTCGAAG 
ACGCGGGCCG AGTGCAAGCG AGCCAAAAAC TCCAAAAATC AGGCTCTGCA AGAGCAAACG 
GGCGAGGCCC ATGTACAAGT GCATCATGTG GAGGCGGAGC TGGGCAAGCT GCAGCTGAGT 
CAAGTGGACC AGCAGAATCA GAATCAGAAC CAGAGCCAAA ACCAGCAGCA TGGTGTGAGT 
GCTACCGGTA GTGGAGCAAG TGCATCCAGC ACGCAGCAGC TGCCCGTGCG GGGATATACC 
CCATCGCCGC CATCGACGGC TCCGCTGCCG ATGAAATTTC CCGGCAAACA TGATCGCTAC 
CTGAACATGA ACAGCATCAG GAGTGCACCC AATCTGCCCG CCGCCAATCC AGCGCATCCC 
CGTCTCCGGG ATCTCCGTCT GCCCATCCAG TCCATGCGGC ATCAGTGCGG CGGCAGCAGC 
AATGACAACA GTCTCACCGA CAGCGCCTAC GTGAATCCGC CCTCGACCAA CTCGAATTCG 
AATGCGGCAT CGGCTTCCGC CTCGGCCTCC GTCTCGGCAA CCGGATCAGC AGCTCCACGC 
CGACCGCGAT CTTTTGTCCT CGAATCGGGC CGGGTGCTAG AGCTGCGACG CAGTGCCCAG 
GGCCATCAGG GTGGCCAGTC GACCCACCAT CACCATCATC ATCATCATCA TGCGCCGGGG 
AGCAGAAGAA ATCGCCATGG CCAAAGCCAG GGGCAAACGA CCGGTGGTGG CCACAACTAC 
AGCAGCACCG AGAGCATTGC CACCTCGAGC AGTGGCGGCA GCATGGAATC GCTGCGTTCC 
AGCACCAGCG AGGGCAATCG CAGCACCTCC AGCTCCGAGT CGCGGCACTC CAGTTCGCTG 
AGCTCTCACA GCTCGGAGAG CGGCAGCGGT GGCAGCGGCG GTAGCGGAGG CTCCAGTGTG 
GCCTATCCCC TGCGACCGGC TCCCCTGCTG CTACACTCCA AGCTGCACAT CCTGAGTCCC 
ATTTCCGACA AGTCCTCGCA GGAACCGGCA TCGGCCAGTG CCTCGGAGCA ATCACAGCAG 
CCGTCCCAGC AGCTGGCGCC GATGGCGTCG CAGGAAGACG ATTCCACAAC GAGTGGCGCA 
CCAGCAGGGA ACCAAGTCCA GGTGAATGCC TCCTTGAAGC AGCCGCGTAG CAATCGGGGT 
GCACCAAATA AGGCCCTGCT CCAGCTGGCC GATGAGCTAC TGGGTTCGGA TAGTGGGATA 
TCGCTGCATT CCCGGGAGGA TGGAAAACCG CAGGCCTTAG CGCTACAGCG TTTAACGCTG 
CCCAAGCTAC AGTTGATTGG TGCTGAAGGA ACAACAACGT CGGCAACGGC CTCCGGAGCA 
GGAGGTAGCT CAGCAACGGG TGGCAGTAGC GGCGCTGGAG GATCAGGATC CGGTTCGGTA 
GTTGCCAGCG GATCGACTGG CATACAGCAG GATCTGCGCG ATTTGCCCTT CGATATGCCC 
AAACTGAGGC GACGCAAGAC GCTGCAACAG GAGGCCGCCT GCACATCGGG CAGTGCCACG 
TCCGTCGATC TCGGCGAGCT GCCCTTCGAC ATGCCCAAGT TGCGACGACG CCTGAGGGCC 
AACCAGGCGG AGATCAATAA CCTGCTGATG CACAGCACCG AATCCAGTGG CATCTCGCAG 
GCTTCCTCCA GTCATTCGAT GCGCGACGAC CAAAAGATGT CCAGCAAGTT GGATACGGCA 
CTTTTTCGGC AGAATCTCAC TCTAAACCTT AATGAATCGC GACAGGCGAC CAAACAGTTT 
GGCAGCCTGG ATCTGCGTGG TCTCAATAGC AACAAGGAGC TAAACATGAA TCTCAGCCAG 
GGATATGTCA CTGCTGTGGA TCTAATCGAT GTGACAATTC CGCTGGAGCG GCAGGGTTGG 
TACCATGGTG CCATAACGCG AATTGAGGCG GAGACCACAC TGCGTCCGCT GTCCGAGGGA 
TCCTTCCTGG TGCGCAACTG CGAGTCCACG AAGCAGGACT ATTCGCTATC CCTCAAGGGC 
GCCAAGGGCT TTATGCACAT GCGCATCCAG CGCAACGAGA CTGGACAGTA CATCCTTGGC 
CAGTTTAGTC GTCCCTTCGA GACGGTACCG GAGATGATCC GGCACTTTTG CCTCAACCGG 
CTGCCAGTGC GCGGTGCCGA GCACATGTGT CTGATCGAGC CGGTCATCGC CCAGCTGCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572806.2
CDS	1999..4641
Translation	MPPHRLLGPAAVCGACITSAPASSLAGGRGSELGDDLEEEYFRPRSIFYVHPHGVHECIECAQPAQPATVPAPTLSNPSLDIYGDDEDEVDGDEDEDEEDDSEDIMGSRRRQIYETAFDCKIAKSDDDLDEVDRITNCSVLLQLSNGNGGDISKVTGSSKTRAECKRAKNSKNQALQEQTGEAHVQVHHVEAELGKLQLSQVDQQNQNQNQSQNQQHGVSATGSGASASSTQQLPVRGYTPSPPSTAPLPMKFPGKHDRYLNMNSIRSAPNLPAANPAHPRLRDLRLPIQSMRHQCGGSSNDNSLTDSAYVNPPSTNSNSNAASASASASVSATGSAAPRRPRSFVLESGRVLELRRSAQGHQGGQSTHHHHHHHHHAPGSRRNRHGQSQGQTTGGGHNYSSTESIATSSSGGSMESLRSSTSEGNRSTSSSESRHSSSLSSHSSESGSGGSGGSGGSSVAYPLRPAPLLLHSKLHILSPISDKSSQEPASASASEQSQQPSQQLAPMASQEDDSTTSGAPAGNQVQVNASLKQPRSNRGAPNKALLQLADELLGSDSGISLHSREDGKPQALALQRLTLPKLQLIGAEGTTTSATASGAGGSSATGGSSGAGGSGSGSVVASGSTGIQQDLRDLPFDMPKLRRRKTLQQEAACTSGSATSVDLGELPFDMPKLRRRLRANQAEINNLLMHSTESSGISQASSSHSMRDDQKMSSKLDTALFRQNLTLNLNESRQATKQFGSLDLRGLNSNKELNMNLSQGYVTAVDLIDVTIPLERQGWYHGAITRIEAETTLRPLSEGSFLVRNCESTKQDYSLSLKGAKGFMHMRIQRNETGQYILGQFSRPFETVPEMIRHFCLNRLPVRGAEHMCLIEPVIAQLL

Target ORF information:

RefSeq Version	NM_132578.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heavyweight (hwt), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132578.3

ORF Insert Sequence:

ATGCCACCGC ACCGCCTGCT GGGACCAGCA GCCGTGTGCG GAGCCTGCAT CACATCCGCT 
CCTGCCTCCT CCTTGGCCGG CGGACGGGGC AGCGAGCTCG GCGATGATCT GGAAGAGGAG 
TACTTCCGGC CGCGCAGCAT CTTCTATGTG CATCCACATG GCGTCCACGA GTGCATCGAG 
TGCGCCCAGC CAGCCCAGCC TGCGACTGTT CCAGCGCCAA CGCTGAGCAA TCCATCGCTC 
GATATCTACG GCGACGACGA GGACGAAGTG GACGGTGACG AGGACGAGGA CGAAGAGGAC 
GACTCCGAGG ACATCATGGG CAGCCGGCGG CGGCAGATCT ATGAGACGGC CTTCGACTGC 
AAGATAGCCA AGTCGGATGA CGATCTAGAT GAGGTGGATC GCATTACCAA TTGCTCGGTC 
TTGCTGCAGT TGAGTAATGG CAACGGTGGT GACATCAGCA AGGTAACCGG CTCTTCGAAG 
ACGCGGGCCG AGTGCAAGCG AGCCAAAAAC TCCAAAAATC AGGCTCTGCA AGAGCAAACG 
GGCGAGGCCC ATGTACAAGT GCATCATGTG GAGGCGGAGC TGGGCAAGCT GCAGCTGAGT 
CAAGTGGACC AGCAGAATCA GAATCAGAAC CAGAGCCAAA ACCAGCAGCA TGGTGTGAGT 
GCTACCGGTA GTGGAGCAAG TGCATCCAGC ACGCAGCAGC TGCCCGTGCG GGGATATACC 
CCATCGCCGC CATCGACGGC TCCGCTGCCG ATGAAATTTC CCGGCAAACA TGATCGCTAC 
CTGAACATGA ACAGCATCAG GAGTGCACCC AATCTGCCCG CCGCCAATCC AGCGCATCCC 
CGTCTCCGGG ATCTCCGTCT GCCCATCCAG TCCATGCGGC ATCAGTGCGG CGGCAGCAGC 
AATGACAACA GTCTCACCGA CAGCGCCTAC GTGAATCCGC CCTCGACCAA CTCGAATTCG 
AATGCGGCAT CGGCTTCCGC CTCGGCCTCC GTCTCGGCAA CCGGATCAGC AGCTCCACGC 
CGACCGCGAT CTTTTGTCCT CGAATCGGGC CGGGTGCTAG AGCTGCGACG CAGTGCCCAG 
GGCCATCAGG GTGGCCAGTC GACCCACCAT CACCATCATC ATCATCATCA TGCGCCGGGG 
AGCAGAAGAA ATCGCCATGG CCAAAGCCAG GGGCAAACGA CCGGTGGTGG CCACAACTAC 
AGCAGCACCG AGAGCATTGC CACCTCGAGC AGTGGCGGCA GCATGGAATC GCTGCGTTCC 
AGCACCAGCG AGGGCAATCG CAGCACCTCC AGCTCCGAGT CGCGGCACTC CAGTTCGCTG 
AGCTCTCACA GCTCGGAGAG CGGCAGCGGT GGCAGCGGCG GTAGCGGAGG CTCCAGTGTG 
GCCTATCCCC TGCGACCGGC TCCCCTGCTG CTACACTCCA AGCTGCACAT CCTGAGTCCC 
ATTTCCGACA AGTCCTCGCA GGAACCGGCA TCGGCCAGTG CCTCGGAGCA ATCACAGCAG 
CCGTCCCAGC AGCTGGCGCC GATGGCGTCG CAGGAAGACG ATTCCACAAC GAGTGGCGCA 
CCAGCAGGGA ACCAAGTCCA GGTGAATGCC TCCTTGAAGC AGCCGCGTAG CAATCGGGGT 
GCACCAAATA AGGCCCTGCT CCAGCTGGCC GATGAGCTAC TGGGTTCGGA TAGTGGGATA 
TCGCTGCATT CCCGGGAGGA TGGAAAACCG CAGGCCTTAG CGCTACAGCG TTTAACGCTG 
CCCAAGCTAC AGTTGATTGG TGCTGAAGGA ACAACAACGT CGGCAACGGC CTCCGGAGCA 
GGAGGTAGCT CAGCAACGGG TGGCAGTAGC GGCGCTGGAG GATCAGGATC CGGTTCGGTA 
GTTGCCAGCG GATCGACTGG CATACAGCAG GATCTGCGCG ATTTGCCCTT CGATATGCCC 
AAACTGAGGC GACGCAAGAC GCTGCAACAG GAGGCCGCCT GCACATCGGG CAGTGCCACG 
TCCGTCGATC TCGGCGAGCT GCCCTTCGAC ATGCCCAAGT TGCGACGACG CCTGAGGGCC 
AACCAGGCGG AGATCAATAA CCTGCTGATG CACAGCACCG AATCCAGTGG CATCTCGCAG 
GCTTCCTCCA GTCATTCGAT GCGCGACGAC CAAAAGATGT CCAGCAAGTT GGATACGGCA 
CTTTTTCGGC AGAATCTCAC TCTAAACCTT AATGAATCGC GACAGGCGAC CAAACAGTTT 
GGCAGCCTGG ATCTGCGTGG TCTCAATAGC AACAAGGAGC TAAACATGAA TCTCAGCCAG 
GGATATGTCA CTGCTGTGGA TCTAATCGAT GTGACAATTC CGCTGGAGCG GCAGGGTTGG 
TACCATGGTG CCATAACGCG AATTGAGGCG GAGACCACAC TGCGTCCGCT GTCCGAGGGA 
TCCTTCCTGG TGCGCAACTG CGAGTCCACG AAGCAGGACT ATTCGCTATC CCTCAAGGGC 
GCCAAGGGCT TTATGCACAT GCGCATCCAG CGCAACGAGA CTGGACAGTA CATCCTTGGC 
CAGTTTAGTC GTCCCTTCGA GACGGTACCG GAGATGATCC GGCACTTTTG CCTCAACCGG 
CTGCCAGTGC GCGGTGCCGA GCACATGTGT CTGATCGAGC CGGTCATCGC CCAGCTGCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hwt ( NM_167324.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727617.2 Drosophila melanogaster heavyweight (hwt), transcript variant B, partial mRNA.

Price & Availability↑

CloneID	OFa01401
Clone ID Related Accession (Same CDS sequence)	NM_167324.3
Accession Version	NM_167324.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heavyweight, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167324.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGTTCTGTC GTCTCTGCTG GATTGATTTT AATGCCGGCG AGGCAATGGT GGAGTGCGCG 
GGTCCCTGTG GTAGTTGCTT CCACCGCAAT TGTGTCGATT GTGTCACCGG GGATGTGGCG 
CACATCCTGG CCACCGGCGG TGGTTACTAT TCCGGTCTGG AATGGTATTG CCGCACTTGT 
CGTCAACTTT ATCGCCTGCA AGTGTACTTC GAGATTGCCA TCTGTGATGA TTACAGCCTG 
CCCGTAGACT TTGTTAAAAA ACGACCGTCC AGCTTTGATC CGTGCCTGGC GGAGGCGATA 
CCAGCGAATC CCGAGCATTG GATAGCACCC AGTCCGCAAA CCGAGTTTCT GCCATTTGGT 
GAAGCCCAAC AAGCGTTGCA GCTACAACCA GCAGCAGCAG CAGCAACAGC AGCAGCAACA 
ACATCTGCAG CAGCAGCGGC AAACGCAACA GCAGCAGCTC CTCAACTACC GCCACCGCTG 
CCCGTTCCCG TGGCAACATT GCCCAATCAG GCGGCGACGG CAGCAGCAGC AGCAACAGCT 
GCGATTTTGG CCACTCACCA GCAACAGCAG CAGCAGCAGC ATCAGCATTT GAGCAACAAC 
AACAACAACG GCGGCAGCAC ACACGGCCAA CAGCATACGC ACAGCAACAA TAACAACACA 
TTGCCAGTAG GGGCACAGCA GCAGCAACAG CAGCAGCATC AACAACAACG TGCAACATTG 
CAACATCCAC CGCTGCCCAA CAGCAACATC AAGCAGGCAT CGGTGAAATT AATCGAAGGA 
TATTCAGATC CAAAGGATAC GCGCAATCAC AAAAAGGTCT ATCACAAAAA GGCCAAGAAA 
CGCTCGACTA CTGGCCACAA GCGGCATCAG CAACAGCAGC AGCAACAGCA GCCGCATCAG 
CAGCAATTGC AGCAGCAGCA ACAGCAAACG TTGCCAACGC CTAGAAGTGC GCAGCAGCAA 
CAGCAACTGC AGCAAAAGTT ACAGCAGCGG CATTCCACCT ACAACAACAA CAACGGCAAC 
TCAAGAAAGG ATAAACGCAA CAGTCAATAC AATTCAGACT CCTCGGCTCC GAAATCCTCC 
GACGACGAGG CGGACATCGA CGAAGAGGTT GAGCAGCAAC CGCTGCCCGT GCCACAAGCA 
CCTGTCCAGA GTGCTTCACA ACCAGCGGCC GGACTCAACT CGCCGGATTC CATATCCGAT 
GACCTGGTGC GTCCGCTTCC ACCGCAACTG CAGCGCAGTC CGCGCAAACT GACCGTCGCT 
CCGGCGGAGC ACAACAAGCG CCAGCCCTCG CCCTACTACT ACTCCGATCT GCTCAAGAGT 
CGTGACAAGG ACAAGGATAA AGACAAGGAC AAGGACAAAG ACAAGGACAA GGATAAAGAC 
AAGGACAAGG ACAAGGACAA GGAACGGGAC AAGGACAGGG AGGACTTTAA GCTCAAGTGT 
CGCCAGTCGA CGGCCAGTGA ACCGCCGCAG CAAACCCAGA CACAGTTGGG TGGCTACCGG 
AAGAGCTCCA GCTTGGATGT GCCGCCGGCG GAGGAGCAGC CGGAAGCAGA GAGTCACGAA 
GAGGAGGATC CCCGGACGGG ACAGGACTCT CAATCCACGC CCAAGCGTTA CAGTTTCACC 
GAGGAGGGTG TACACATCAT TCGCTGCGAG ACGCCCAGCA CCAGTACTTC CGAGGAATCC 
GATTGCAGTG AGTGCCTCAA GCGACGCGAG TGGCACGCTC GTGCCTTAGC CTTAGTCCGC 
AAGACCTGCA ACGTTCAGCC AAGGAATCAG AATCCCACTG GCAGCGAGCT TCAGCTGCAG 
TTGCAGCATT GCGACGCCGG CGAGGGCGAA CTGCTTAAGT GCTGTCCACC GCCACCGGCT 
CCAGCGGAGA ACCAGTCTCA GGTCCAGCCG ATGCCACCGC ACCGCCTGCT GGGACCAGCA 
GCCGTGTGCG GAGCCTGCAT CACATCCGCT CCTGCCTCCT CCTTGGCCGG CGGACGGGGC 
AGCGAGCTCG GCGATGATCT GGAAGAGGAG TACTTCCGGC CGCGCAGCAT CTTCTATGTG 
CATCCACATG GCGTCCACGA GTGCATCGAG TGCGCCCAGC CAGCCCAGCC TGCGACTGTT 
CCAGCGCCAA CGCTGAGCAA TCCATCGCTC GATATCTACG GCGACGACGA GGACGAAGTG 
GACGGTGACG AGGACGAGGA CGAAGAGGAC GACTCCGAGG ACATCATGGG CAGCCGGCGG 
CGGCAGATCT ATGAGACGGC CTTCGACTGC AAGATAGCCA AGTCGGATGA CGATCTAGAT 
GAGGTGGATC GCATTACCAA TTGCTCGGTC TTGCTGCAGT TGAGTAATGG CAACGGTGGT 
GACATCAGCA AGGTAACCGG CTCTTCGAAG ACGCGGGCCG AGTGCAAGCG AGCCAAAAAC 
TCCAAAAATC AGGCTCTGCA AGAGCAAACG GGCGAGGCCC ATGTACAAGT GCATCATGTG 
GAGGCGGAGC TGGGCAAGCT GCAGCTGAGT CAAGTGGACC AGCAGAATCA GAATCAGAAC 
CAGAGCCAAA ACCAGCAGCA TGGTGTGAGT GCTACCGGTA GTGGAGCAAG TGCATCCAGC 
ACGCAGCAGC TGCCCGTGCG GGGATATACC CCATCGCCGC CATCGACGGC TCCGCTGCCG 
ATGAAATTTC CCGGCAAACA TGATCGCTAC CTGAACATGA ACAGCATCAG GAGTGCACCC 
AATCTGCCCG CCGCCAATCC AGCGCATCCC CGTCTCCGGG ATCTCCGTCT GCCCATCCAG 
TCCATGCGGC ATCAGTGCGG CGGCAGCAGC AATGACAACA GTCTCACCGA CAGCGCCTAC 
GTGAATCCGC CCTCGACCAA CTCGAATTCG AATGCGGCAT CGGCTTCCGC CTCGGCCTCC 
GTCTCGGCAA CCGGATCAGC AGCTCCACGC CGACCGCGAT CTTTTGTCCT CGAATCGGGC 
CGGGTGCTAG AGCTGCGACG CAGTGCCCAG GGCCATCAGG GTGGCCAGTC GACCCACCAT 
CACCATCATC ATCATCATCA TGCGCCGGGG AGCAGAAGAA ATCGCCATGG CCAAAGCCAG 
GGGCAAACGA CCGGTGGTGG CCACAACTAC AGCAGCACCG AGAGCATTGC CACCTCGAGC 
AGTGGCGGCA GCATGGAATC GCTGCGTTCC AGCACCAGCG AGGGCAATCG CAGCACCTCC 
AGCTCCGAGT CGCGGCACTC CAGTTCGCTG AGCTCTCACA GCTCGGAGAG CGGCAGCGGT 
GGCAGCGGCG GTAGCGGAGG CTCCAGTGTG GCCTATCCCC TGCGACCGGC TCCCCTGCTG 
CTACACTCCA AGCTGCACAT CCTGAGTCCC ATTTCCGACA AGTCCTCGCA GGAACCGGCA 
TCGGCCAGTG CCTCGGAGCA ATCACAGCAG CCGTCCCAGC AGCTGGCGCC GATGGCGTCG 
CAGGAAGACG ATTCCACAAC GAGTGGCGCA CCAGCAGGGA ACCAAGTCCA GGTGAATGCC 
TCCTTGAAGC AGCCGCGTAG CAATCGGGGT GCACCAAATA AGGCCCTGCT CCAGCTGGCC 
GATGAGCTAC TGGGTTCGGA TAGTGGGATA TCGCTGCATT CCCGGGAGGA TGGAAAACCG 
CAGGCCTTAG CGCTACAGCG TTTAACGCTG CCCAAGCTAC AGTTGATTGG TGCTGAAGGA 
ACAACAACGT CGGCAACGGC CTCCGGAGCA GGAGGTAGCT CAGCAACGGG TGGCAGTAGC 
GGCGCTGGAG GATCAGGATC CGGTTCGGTA GTTGCCAGCG GATCGACTGG CATACAGCAG 
GATCTGCGCG ATTTGCCCTT CGATATGCCC AAACTGAGGC GACGCAAGAC GCTGCAACAG 
GAGGCCGCCT GCACATCGGG CAGTGCCACG TCCGTCGATC TCGGCGAGCT GCCCTTCGAC 
ATGCCCAAGT TGCGACGACG CCTGAGGGCC AACCAGGCGG AGATCAATAA CCTGCTGATG 
CACAGCACCG AATCCAGTGG CATCTCGCAG GCTTCCTCCA GTCATTCGAT GCGCGACGAC 
CAAAAGATGT CCAGCAAGTT GGATACGGCA CTTTTTCGGC AGAATCTCAC TCTAAACCTT 
AATGAATCGC GACAGGCGAC CAAACAGTTT GGCAGCCTGG ATCTGCGTGG TCTCAATAGC 
AACAAGGAGC TAAACATGAA TCTCAGCCAG GGATATGTCA CTGCTGTGGA TCTAATCGAT 
GTGACAATTC CGCTGGAGCG GCAGGGTTGG TACCATGGTG CCATAACGCG AATTGAGGCG 
GAGACCACAC TGCGTCCGCT GTCCGAGGGA TCCTTCCTGG TGCGCAACTG CGAGTCCACG 
AAGCAGGACT ATTCGCTATC CCTCAAGGGC GCCAAGGGCT TTATGCACAT GCGCATCCAG 
CGCAACGAGA CTGGACAGTA CATCCTTGGC CAGTTTAGTC GTCCCTTCGA GACGGTACCG 
GAGATGATCC GGCACTTTTG CCTCAACCGG CTGCCAGTGC GCGGTGCCGA GCACATGTGT 
CTGATCGAGC CGGTCATCGC CCAGCTGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727617.2
CDS	1..4533
Translation	MFCRLCWIDFNAGEAMVECAGPCGSCFHRNCVDCVTGDVAHILATGGGYYSGLEWYCRTCRQLYRLQVYFEIAICDDYSLPVDFVKKRPSSFDPCLAEAIPANPEHWIAPSPQTEFLPFGEAQQALQLQPAAAAATAAATTSAAAAANATAAAPQLPPPLPVPVATLPNQAATAAAAATAAILATHQQQQQQQHQHLSNNNNNGGSTHGQQHTHSNNNNTLPVGAQQQQQQQHQQQRATLQHPPLPNSNIKQASVKLIEGYSDPKDTRNHKKVYHKKAKKRSTTGHKRHQQQQQQQQPHQQQLQQQQQQTLPTPRSAQQQQQLQQKLQQRHSTYNNNNGNSRKDKRNSQYNSDSSAPKSSDDEADIDEEVEQQPLPVPQAPVQSASQPAAGLNSPDSISDDLVRPLPPQLQRSPRKLTVAPAEHNKRQPSPYYYSDLLKSRDKDKDKDKDKDKDKDKDKDKDKDKDKERDKDREDFKLKCRQSTASEPPQQTQTQLGGYRKSSSLDVPPAEEQPEAESHEEEDPRTGQDSQSTPKRYSFTEEGVHIIRCETPSTSTSEESDCSECLKRREWHARALALVRKTCNVQPRNQNPTGSELQLQLQHCDAGEGELLKCCPPPPAPAENQSQVQPMPPHRLLGPAAVCGACITSAPASSLAGGRGSELGDDLEEEYFRPRSIFYVHPHGVHECIECAQPAQPATVPAPTLSNPSLDIYGDDEDEVDGDEDEDEEDDSEDIMGSRRRQIYETAFDCKIAKSDDDLDEVDRITNCSVLLQLSNGNGGDISKVTGSSKTRAECKRAKNSKNQALQEQTGEAHVQVHHVEAELGKLQLSQVDQQNQNQNQSQNQQHGVSATGSGASASSTQQLPVRGYTPSPPSTAPLPMKFPGKHDRYLNMNSIRSAPNLPAANPAHPRLRDLRLPIQSMRHQCGGSSNDNSLTDSAYVNPPSTNSNSNAASASASASVSATGSAAPRRPRSFVLESGRVLELRRSAQGHQGGQSTHHHHHHHHHAPGSRRNRHGQSQGQTTGGGHNYSSTESIATSSSGGSMESLRSSTSEGNRSTSSSESRHSSSLSSHSSESGSGGSGGSGGSSVAYPLRPAPLLLHSKLHILSPISDKSSQEPASASASEQSQQPSQQLAPMASQEDDSTTSGAPAGNQVQVNASLKQPRSNRGAPNKALLQLADELLGSDSGISLHSREDGKPQALALQRLTLPKLQLIGAEGTTTSATASGAGGSSATGGSSGAGGSGSGSVVASGSTGIQQDLRDLPFDMPKLRRRKTLQQEAACTSGSATSVDLGELPFDMPKLRRRLRANQAEINNLLMHSTESSGISQASSSHSMRDDQKMSSKLDTALFRQNLTLNLNESRQATKQFGSLDLRGLNSNKELNMNLSQGYVTAVDLIDVTIPLERQGWYHGAITRIEAETTLRPLSEGSFLVRNCESTKQDYSLSLKGAKGFMHMRIQRNETGQYILGQFSRPFETVPEMIRHFCLNRLPVRGAEHMCLIEPVIAQLL

Target ORF information:

RefSeq Version	NM_167324.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heavyweight (hwt), transcript variant B, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167324.3

ORF Insert Sequence:

ATGTTCTGTC GTCTCTGCTG GATTGATTTT AATGCCGGCG AGGCAATGGT GGAGTGCGCG 
GGTCCCTGTG GTAGTTGCTT CCACCGCAAT TGTGTCGATT GTGTCACCGG GGATGTGGCG 
CACATCCTGG CCACCGGCGG TGGTTACTAT TCCGGTCTGG AATGGTATTG CCGCACTTGT 
CGTCAACTTT ATCGCCTGCA AGTGTACTTC GAGATTGCCA TCTGTGATGA TTACAGCCTG 
CCCGTAGACT TTGTTAAAAA ACGACCGTCC AGCTTTGATC CGTGCCTGGC GGAGGCGATA 
CCAGCGAATC CCGAGCATTG GATAGCACCC AGTCCGCAAA CCGAGTTTCT GCCATTTGGT 
GAAGCCCAAC AAGCGTTGCA GCTACAACCA GCAGCAGCAG CAGCAACAGC AGCAGCAACA 
ACATCTGCAG CAGCAGCGGC AAACGCAACA GCAGCAGCTC CTCAACTACC GCCACCGCTG 
CCCGTTCCCG TGGCAACATT GCCCAATCAG GCGGCGACGG CAGCAGCAGC AGCAACAGCT 
GCGATTTTGG CCACTCACCA GCAACAGCAG CAGCAGCAGC ATCAGCATTT GAGCAACAAC 
AACAACAACG GCGGCAGCAC ACACGGCCAA CAGCATACGC ACAGCAACAA TAACAACACA 
TTGCCAGTAG GGGCACAGCA GCAGCAACAG CAGCAGCATC AACAACAACG TGCAACATTG 
CAACATCCAC CGCTGCCCAA CAGCAACATC AAGCAGGCAT CGGTGAAATT AATCGAAGGA 
TATTCAGATC CAAAGGATAC GCGCAATCAC AAAAAGGTCT ATCACAAAAA GGCCAAGAAA 
CGCTCGACTA CTGGCCACAA GCGGCATCAG CAACAGCAGC AGCAACAGCA GCCGCATCAG 
CAGCAATTGC AGCAGCAGCA ACAGCAAACG TTGCCAACGC CTAGAAGTGC GCAGCAGCAA 
CAGCAACTGC AGCAAAAGTT ACAGCAGCGG CATTCCACCT ACAACAACAA CAACGGCAAC 
TCAAGAAAGG ATAAACGCAA CAGTCAATAC AATTCAGACT CCTCGGCTCC GAAATCCTCC 
GACGACGAGG CGGACATCGA CGAAGAGGTT GAGCAGCAAC CGCTGCCCGT GCCACAAGCA 
CCTGTCCAGA GTGCTTCACA ACCAGCGGCC GGACTCAACT CGCCGGATTC CATATCCGAT 
GACCTGGTGC GTCCGCTTCC ACCGCAACTG CAGCGCAGTC CGCGCAAACT GACCGTCGCT 
CCGGCGGAGC ACAACAAGCG CCAGCCCTCG CCCTACTACT ACTCCGATCT GCTCAAGAGT 
CGTGACAAGG ACAAGGATAA AGACAAGGAC AAGGACAAAG ACAAGGACAA GGATAAAGAC 
AAGGACAAGG ACAAGGACAA GGAACGGGAC AAGGACAGGG AGGACTTTAA GCTCAAGTGT 
CGCCAGTCGA CGGCCAGTGA ACCGCCGCAG CAAACCCAGA CACAGTTGGG TGGCTACCGG 
AAGAGCTCCA GCTTGGATGT GCCGCCGGCG GAGGAGCAGC CGGAAGCAGA GAGTCACGAA 
GAGGAGGATC CCCGGACGGG ACAGGACTCT CAATCCACGC CCAAGCGTTA CAGTTTCACC 
GAGGAGGGTG TACACATCAT TCGCTGCGAG ACGCCCAGCA CCAGTACTTC CGAGGAATCC 
GATTGCAGTG AGTGCCTCAA GCGACGCGAG TGGCACGCTC GTGCCTTAGC CTTAGTCCGC 
AAGACCTGCA ACGTTCAGCC AAGGAATCAG AATCCCACTG GCAGCGAGCT TCAGCTGCAG 
TTGCAGCATT GCGACGCCGG CGAGGGCGAA CTGCTTAAGT GCTGTCCACC GCCACCGGCT 
CCAGCGGAGA ACCAGTCTCA GGTCCAGCCG ATGCCACCGC ACCGCCTGCT GGGACCAGCA 
GCCGTGTGCG GAGCCTGCAT CACATCCGCT CCTGCCTCCT CCTTGGCCGG CGGACGGGGC 
AGCGAGCTCG GCGATGATCT GGAAGAGGAG TACTTCCGGC CGCGCAGCAT CTTCTATGTG 
CATCCACATG GCGTCCACGA GTGCATCGAG TGCGCCCAGC CAGCCCAGCC TGCGACTGTT 
CCAGCGCCAA CGCTGAGCAA TCCATCGCTC GATATCTACG GCGACGACGA GGACGAAGTG 
GACGGTGACG AGGACGAGGA CGAAGAGGAC GACTCCGAGG ACATCATGGG CAGCCGGCGG 
CGGCAGATCT ATGAGACGGC CTTCGACTGC AAGATAGCCA AGTCGGATGA CGATCTAGAT 
GAGGTGGATC GCATTACCAA TTGCTCGGTC TTGCTGCAGT TGAGTAATGG CAACGGTGGT 
GACATCAGCA AGGTAACCGG CTCTTCGAAG ACGCGGGCCG AGTGCAAGCG AGCCAAAAAC 
TCCAAAAATC AGGCTCTGCA AGAGCAAACG GGCGAGGCCC ATGTACAAGT GCATCATGTG 
GAGGCGGAGC TGGGCAAGCT GCAGCTGAGT CAAGTGGACC AGCAGAATCA GAATCAGAAC 
CAGAGCCAAA ACCAGCAGCA TGGTGTGAGT GCTACCGGTA GTGGAGCAAG TGCATCCAGC 
ACGCAGCAGC TGCCCGTGCG GGGATATACC CCATCGCCGC CATCGACGGC TCCGCTGCCG 
ATGAAATTTC CCGGCAAACA TGATCGCTAC CTGAACATGA ACAGCATCAG GAGTGCACCC 
AATCTGCCCG CCGCCAATCC AGCGCATCCC CGTCTCCGGG ATCTCCGTCT GCCCATCCAG 
TCCATGCGGC ATCAGTGCGG CGGCAGCAGC AATGACAACA GTCTCACCGA CAGCGCCTAC 
GTGAATCCGC CCTCGACCAA CTCGAATTCG AATGCGGCAT CGGCTTCCGC CTCGGCCTCC 
GTCTCGGCAA CCGGATCAGC AGCTCCACGC CGACCGCGAT CTTTTGTCCT CGAATCGGGC 
CGGGTGCTAG AGCTGCGACG CAGTGCCCAG GGCCATCAGG GTGGCCAGTC GACCCACCAT 
CACCATCATC ATCATCATCA TGCGCCGGGG AGCAGAAGAA ATCGCCATGG CCAAAGCCAG 
GGGCAAACGA CCGGTGGTGG CCACAACTAC AGCAGCACCG AGAGCATTGC CACCTCGAGC 
AGTGGCGGCA GCATGGAATC GCTGCGTTCC AGCACCAGCG AGGGCAATCG CAGCACCTCC 
AGCTCCGAGT CGCGGCACTC CAGTTCGCTG AGCTCTCACA GCTCGGAGAG CGGCAGCGGT 
GGCAGCGGCG GTAGCGGAGG CTCCAGTGTG GCCTATCCCC TGCGACCGGC TCCCCTGCTG 
CTACACTCCA AGCTGCACAT CCTGAGTCCC ATTTCCGACA AGTCCTCGCA GGAACCGGCA 
TCGGCCAGTG CCTCGGAGCA ATCACAGCAG CCGTCCCAGC AGCTGGCGCC GATGGCGTCG 
CAGGAAGACG ATTCCACAAC GAGTGGCGCA CCAGCAGGGA ACCAAGTCCA GGTGAATGCC 
TCCTTGAAGC AGCCGCGTAG CAATCGGGGT GCACCAAATA AGGCCCTGCT CCAGCTGGCC 
GATGAGCTAC TGGGTTCGGA TAGTGGGATA TCGCTGCATT CCCGGGAGGA TGGAAAACCG 
CAGGCCTTAG CGCTACAGCG TTTAACGCTG CCCAAGCTAC AGTTGATTGG TGCTGAAGGA 
ACAACAACGT CGGCAACGGC CTCCGGAGCA GGAGGTAGCT CAGCAACGGG TGGCAGTAGC 
GGCGCTGGAG GATCAGGATC CGGTTCGGTA GTTGCCAGCG GATCGACTGG CATACAGCAG 
GATCTGCGCG ATTTGCCCTT CGATATGCCC AAACTGAGGC GACGCAAGAC GCTGCAACAG 
GAGGCCGCCT GCACATCGGG CAGTGCCACG TCCGTCGATC TCGGCGAGCT GCCCTTCGAC 
ATGCCCAAGT TGCGACGACG CCTGAGGGCC AACCAGGCGG AGATCAATAA CCTGCTGATG 
CACAGCACCG AATCCAGTGG CATCTCGCAG GCTTCCTCCA GTCATTCGAT GCGCGACGAC 
CAAAAGATGT CCAGCAAGTT GGATACGGCA CTTTTTCGGC AGAATCTCAC TCTAAACCTT 
AATGAATCGC GACAGGCGAC CAAACAGTTT GGCAGCCTGG ATCTGCGTGG TCTCAATAGC 
AACAAGGAGC TAAACATGAA TCTCAGCCAG GGATATGTCA CTGCTGTGGA TCTAATCGAT 
GTGACAATTC CGCTGGAGCG GCAGGGTTGG TACCATGGTG CCATAACGCG AATTGAGGCG 
GAGACCACAC TGCGTCCGCT GTCCGAGGGA TCCTTCCTGG TGCGCAACTG CGAGTCCACG 
AAGCAGGACT ATTCGCTATC CCTCAAGGGC GCCAAGGGCT TTATGCACAT GCGCATCCAG 
CGCAACGAGA CTGGACAGTA CATCCTTGGC CAGTTTAGTC GTCCCTTCGA GACGGTACCG 
GAGATGATCC GGCACTTTTG CCTCAACCGG CTGCCAGTGC GCGGTGCCGA GCACATGTGT 
CTGATCGAGC CGGTCATCGC CCAGCTGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hwt ( NM_001272555.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259484.1 Drosophila melanogaster heavyweight (hwt), transcript variant A, mRNA.

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CloneID	OFa02800
Clone ID Related Accession (Same CDS sequence)	NM_001272555.2
Accession Version	NM_001272555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heavyweight, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272555.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCGCA TGTGGCGAAA AGTAAACCAC AGTTTGCGCA ACACCAGCAA GTCGCAAGCA 
CAGCAAGCAG CAGGTGGCGA TGGAATCATC GGTGGCCCAG CCACAGGAGC CACTGCAGCC 
ACGCCTCAAT CCACCGACAC GATTAGTTCG GCTGGCGGTT TCGGCGATGG CCAGCTGGTG 
GTCGCTGTCC AGGGATTAGC CAGCGGAGGC ATTGGTGCTT CCATCACTGC CAGTGGTGGC 
AGCAATGCCA CCGGACGACG ACTGGCCAGC AGCGGTGGAG GTGGCGGTTG TAACAAATCC 
CTTAGCCAAC ATGTCCTGCA AACACGTACC GCTTCCTCGG AACGCCGCAG GCGACGCCGT 
CGGAAAAGTC GCCCACGACG TGGCGGTGGA ATGGGTGTCA CCAGTTGCGT CGGCGATCCT 
GGTGGCGACA ATATGTCTTC CTCGGGCGGC TTTTATACAC TGAAAGAGCA CCAGCACTAT 
CGTCCCAGTC ACCATCATGG CGGGGGCGTT TCTGTCGGCG TTGGCGGTGG CTCATCCCGT 
CGTCTTTTCG CCACTTCGGC GCCCAGCGGC ACAGTGATGA TGTATCCGAA TCCCCAGCGC 
GGCACGGCAC TAGCACCATC TGTGGGATCT GGAACCGGGC ACGCAAACAC TGCGGGCTTA 
GGAGGAGGAG GAGGAGGAGG AGCCGGAGGT GCTGGAGGTG GAGCGACTGG ATCCGGAACT 
GGAGGACAGC CGGACATTTC ACTGAGACAG CGGGCGGTGG CCAAGTTGCG CATGTTCAAC 
TTCCATCTCA ACTGGGATCT GCACATGACG CACTGCAAGC CCTGCGGTCC CCGTTTGAGT 
GGCAGTGGTG GCAACATAAT CACCCGAAGG CTGTGCCGCA ATCGTCGGCG GGAGGACAAC 
GAACTGTACC GCTCCAATAG CTTCAAGTTC GAGCGATTCG AGCGCAAGGA GTGCCTGGAG 
GAGCTGTCCA ACACGTTGCA GAAGCAGATT GCCATCTGTG ATGATTACAG CCTGCCCGTA 
GACTTTGTTA AAAAACGACC GTCCAGCTTT GATCCGTGCC TGGCGGAGGC GATACCAGCG 
AATCCCGAGC ATTGGATAGC ACCCAGTCCG CAAACCGAGT TTCTGCCATT TGGTGAAGCC 
CAACAAGCGT TGCAGCTACA ACCAGCAGCA GCAGCAGCAA CAGCAGCAGC AACAACATCT 
GCAGCAGCAG CGGCAAACGC AACAGCAGCA GCTCCTCAAC TACCGCCACC GCTGCCCGTT 
CCCGTGGCAA CATTGCCCAA TCAGGCGGCG ACGGCAGCAG CAGCAGCAAC AGCTGCGATT 
TTGGCCACTC ACCAGCAACA GCAGCAGCAG CAGCATCAGC ATTTGAGCAA CAACAACAAC 
AACGGCGGCA GCACACACGG CCAACAGCAT ACGCACAGCA ACAATAACAA CACATTGCCA 
GTAGGGGCAC AGCAGCAGCA ACAGCAGCAG CATCAACAAC AACGTGCAAC ATTGCAACAT 
CCACCGCTGC CCAACAGCAA CATCAAGCAG GCATCGGTGA AATTAATCGA AGGATATTCA 
GATCCAAAGG ATACGCGCAA TCACAAAAAG GTCTATCACA AAAAGGCCAA GAAACGCTCG 
ACTACTGGCC ACAAGCGGCA TCAGCAACAG CAGCAGCAAC AGCAGCCGCA TCAGCAGCAA 
TTGCAGCAGC AGCAACAGCA AACGTTGCCA ACGCCTAGAA GTGCGCAGCA GCAACAGCAA 
CTGCAGCAAA AGTTACAGCA GCGGCATTCC ACCTACAACA ACAACAACGG CAACTCAAGA 
AAGGATAAAC GCAACAGTCA ATACAATTCA GACTCCTCGG CTCCGAAATC CTCCGACGAC 
GAGGCGGACA TCGACGAAGA GGTTGAGCAG CAACCGCTGC CCGTGCCACA AGCACCTGTC 
CAGAGTGCTT CACAACCAGC GGCCGGACTC AACTCGCCGG ATTCCATATC CGATGACCTG 
GTGCGTCCGC TTCCACCGCA ACTGCAGCGC AGTCCGCGCA AACTGACCGT CGCTCCGGCG 
GAGCACAACA AGCGCCAGCC CTCGCCCTAC TACTACTCCG ATCTGCTCAA GAGTCGTGAC 
AAGGACAAGG ATAAAGACAA GGACAAGGAC AAAGACAAGG ACAAGGATAA AGACAAGGAC 
AAGGACAAGG ACAAGGAACG GGACAAGGAC AGGGAGGACT TTAAGCTCAA GTGTCGCCAG 
TCGACGGCCA GTGAACCGCC GCAGCAAACC CAGACACAGT TGGGTGGCTA CCGGAAGAGC 
TCCAGCTTGG ATGTGCCGCC GGCGGAGGAG CAGCCGGAAG CAGAGAGTCA CGAAGAGGAG 
GATCCCCGGA CGGGACAGGA CTCTCAATCC ACGCCCAAGC GTTACAGTTT CACCGAGGAG 
GGTGTACACA TCATTCGCTG CGAGACGCCC AGCACCAGTA CTTCCGAGGA ATCCGATTGC 
AGTGAGTGCC TCAAGCGACG CGAGTGGCAC GCTCGTGCCT TAGCCTTAGT CCGCAAGACC 
TGCAACGTTC AGCCAAGGAA TCAGAATCCC ACTGGCAGCG AGCTTCAGCT GCAGTTGCAG 
CATTGCGACG CCGGCGAGGG CGAACTGCTT AAGTGCTGTC CACCGCCACC GGCTCCAGCG 
GAGAACCAGT CTCAGGTCCA GCCGATGCCA CCGCACCGCC TGCTGGGACC AGCAGCCGTG 
TGCGGAGCCT GCATCACATC CGCTCCTGCC TCCTCCTTGG CCGGCGGACG GGGCAGCGAG 
CTCGGCGATG ATCTGGAAGA GGAGTACTTC CGGCCGCGCA GCATCTTCTA TGTGCATCCA 
CATGGCGTCC ACGAGTGCAT CGAGTGCGCC CAGCCAGCCC AGCCTGCGAC TGTTCCAGCG 
CCAACGCTGA GCAATCCATC GCTCGATATC TACGGCGACG ACGAGGACGA AGTGGACGGT 
GACGAGGACG AGGACGAAGA GGACGACTCC GAGGACATCA TGGGCAGCCG GCGGCGGCAG 
ATCTATGAGA CGGCCTTCGA CTGCAAGATA GCCAAGTCGG ATGACGATCT AGATGAGGTG 
GATCGCATTA CCAATTGCTC GGTCTTGCTG CAGTTGAGTA ATGGCAACGG TGGTGACATC 
AGCAAGGTAA CCGGCTCTTC GAAGACGCGG GCCGAGTGCA AGCGAGCCAA AAACTCCAAA 
AATCAGGCTC TGCAAGAGCA AACGGGCGAG GCCCATGTAC AAGTGCATCA TGTGGAGGCG 
GAGCTGGGCA AGCTGCAGCT GAGTCAAGTG GACCAGCAGA ATCAGAATCA GAACCAGAGC 
CAAAACCAGC AGCATGGTGT GAGTGCTACC GGTAGTGGAG CAAGTGCATC CAGCACGCAG 
CAGCTGCCCG TGCGGGGATA TACCCCATCG CCGCCATCGA CGGCTCCGCT GCCGATGAAA 
TTTCCCGGCA AACATGATCG CTACCTGAAC ATGAACAGCA TCAGGAGTGC ACCCAATCTG 
CCCGCCGCCA ATCCAGCGCA TCCCCGTCTC CGGGATCTCC GTCTGCCCAT CCAGTCCATG 
CGGCATCAGT GCGGCGGCAG CAGCAATGAC AACAGTCTCA CCGACAGCGC CTACGTGAAT 
CCGCCCTCGA CCAACTCGAA TTCGAATGCG GCATCGGCTT CCGCCTCGGC CTCCGTCTCG 
GCAACCGGAT CAGCAGCTCC ACGCCGACCG CGATCTTTTG TCCTCGAATC GGGCCGGGTG 
CTAGAGCTGC GACGCAGTGC CCAGGGCCAT CAGGGTGGCC AGTCGACCCA CCATCACCAT 
CATCATCATC ATCATGCGCC GGGGAGCAGA AGAAATCGCC ATGGCCAAAG CCAGGGGCAA 
ACGACCGGTG GTGGCCACAA CTACAGCAGC ACCGAGAGCA TTGCCACCTC GAGCAGTGGC 
GGCAGCATGG AATCGCTGCG TTCCAGCACC AGCGAGGGCA ATCGCAGCAC CTCCAGCTCC 
GAGTCGCGGC ACTCCAGTTC GCTGAGCTCT CACAGCTCGG AGAGCGGCAG CGGTGGCAGC 
GGCGGTAGCG GAGGCTCCAG TGTGGCCTAT CCCCTGCGAC CGGCTCCCCT GCTGCTACAC 
TCCAAGCTGC ACATCCTGAG TCCCATTTCC GACAAGTCCT CGCAGGAACC GGCATCGGCC 
AGTGCCTCGG AGCAATCACA GCAGCCGTCC CAGCAGCTGG CGCCGATGGC GTCGCAGGAA 
GACGATTCCA CAACGAGTGG CGCACCAGCA GGGAACCAAG TCCAGGTGAA TGCCTCCTTG 
AAGCAGCCGC GTAGCAATCG GGGTGCACCA AATAAGGCCC TGCTCCAGCT GGCCGATGAG 
CTACTGGGTT CGGATAGTGG GATATCGCTG CATTCCCGGG AGGATGGAAA ACCGCAGGCC 
TTAGCGCTAC AGCGTTTAAC GCTGCCCAAG CTACAGTTGA TTGGTGCTGA AGGAACAACA 
ACGTCGGCAA CGGCCTCCGG AGCAGGAGGT AGCTCAGCAA CGGGTGGCAG TAGCGGCGCT 
GGAGGATCAG GATCCGGTTC GGTAGTTGCC AGCGGATCGA CTGGCATACA GCAGGATCTG 
CGCGATTTGC CCTTCGATAT GCCCAAACTG AGGCGACGCA AGACGCTGCA ACAGGAGGCC 
GCCTGCACAT CGGGCAGTGC CACGTCCGTC GATCTCGGCG AGCTGCCCTT CGACATGCCC 
AAGTTGCGAC GACGCCTGAG GGCCAACCAG GCGGAGATCA ATAACCTGCT GATGCACAGC 
ACCGAATCCA GTGGCATCTC GCAGGCTTCC TCCAGTCATT CGATGCGCGA CGACCAAAAG 
ATGTCCAGCA AGTTGGATAC GGCACTTTTT CGGCAGAATC TCACTCTAAA CCTTAATGAA 
TCGCGACAGG CGACCAAACA GTTTGGCAGC CTGGATCTGC GTGGTCTCAA TAGCAACAAG 
GAGCTAAACA TGAATCTCAG CCAGGGATAT GTCACTGCTG TGGATCTAAT CGATGTGACA 
ATTCCGCTGG AGCGGCAGGG TTGGTACCAT GGTGCCATAA CGCGAATTGA GGCGGAGACC 
ACACTGCGTC CGCTGTCCGA GGGATCCTTC CTGGTGCGCA ACTGCGAGTC CACGAAGCAG 
GACTATTCGC TATCCCTCAA GGGCGCCAAG GGCTTTATGC ACATGCGCAT CCAGCGCAAC 
GAGACTGGAC AGTACATCCT TGGCCAGTTT AGTCGTCCCT TCGAGACGGT ACCGGAGATG 
ATCCGGCACT TTTGCCTCAA CCGGCTGCCA GTGCGCGGTG CCGAGCACAT GTGTCTGATC 
GAGCCGGTCA TCGCCCAGCT GCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259484.1
CDS	1550..6856
Translation	MERMWRKVNHSLRNTSKSQAQQAAGGDGIIGGPATGATAATPQSTDTISSAGGFGDGQLVVAVQGLASGGIGASITASGGSNATGRRLASSGGGGGCNKSLSQHVLQTRTASSERRRRRRRKSRPRRGGGMGVTSCVGDPGGDNMSSSGGFYTLKEHQHYRPSHHHGGGVSVGVGGGSSRRLFATSAPSGTVMMYPNPQRGTALAPSVGSGTGHANTAGLGGGGGGGAGGAGGGATGSGTGGQPDISLRQRAVAKLRMFNFHLNWDLHMTHCKPCGPRLSGSGGNIITRRLCRNRRREDNELYRSNSFKFERFERKECLEELSNTLQKQIAICDDYSLPVDFVKKRPSSFDPCLAEAIPANPEHWIAPSPQTEFLPFGEAQQALQLQPAAAAATAAATTSAAAAANATAAAPQLPPPLPVPVATLPNQAATAAAAATAAILATHQQQQQQQHQHLSNNNNNGGSTHGQQHTHSNNNNTLPVGAQQQQQQQHQQQRATLQHPPLPNSNIKQASVKLIEGYSDPKDTRNHKKVYHKKAKKRSTTGHKRHQQQQQQQQPHQQQLQQQQQQTLPTPRSAQQQQQLQQKLQQRHSTYNNNNGNSRKDKRNSQYNSDSSAPKSSDDEADIDEEVEQQPLPVPQAPVQSASQPAAGLNSPDSISDDLVRPLPPQLQRSPRKLTVAPAEHNKRQPSPYYYSDLLKSRDKDKDKDKDKDKDKDKDKDKDKDKDKERDKDREDFKLKCRQSTASEPPQQTQTQLGGYRKSSSLDVPPAEEQPEAESHEEEDPRTGQDSQSTPKRYSFTEEGVHIIRCETPSTSTSEESDCSECLKRREWHARALALVRKTCNVQPRNQNPTGSELQLQLQHCDAGEGELLKCCPPPPAPAENQSQVQPMPPHRLLGPAAVCGACITSAPASSLAGGRGSELGDDLEEEYFRPRSIFYVHPHGVHECIECAQPAQPATVPAPTLSNPSLDIYGDDEDEVDGDEDEDEEDDSEDIMGSRRRQIYETAFDCKIAKSDDDLDEVDRITNCSVLLQLSNGNGGDISKVTGSSKTRAECKRAKNSKNQALQEQTGEAHVQVHHVEAELGKLQLSQVDQQNQNQNQSQNQQHGVSATGSGASASSTQQLPVRGYTPSPPSTAPLPMKFPGKHDRYLNMNSIRSAPNLPAANPAHPRLRDLRLPIQSMRHQCGGSSNDNSLTDSAYVNPPSTNSNSNAASASASASVSATGSAAPRRPRSFVLESGRVLELRRSAQGHQGGQSTHHHHHHHHHAPGSRRNRHGQSQGQTTGGGHNYSSTESIATSSSGGSMESLRSSTSEGNRSTSSSESRHSSSLSSHSSESGSGGSGGSGGSSVAYPLRPAPLLLHSKLHILSPISDKSSQEPASASASEQSQQPSQQLAPMASQEDDSTTSGAPAGNQVQVNASLKQPRSNRGAPNKALLQLADELLGSDSGISLHSREDGKPQALALQRLTLPKLQLIGAEGTTTSATASGAGGSSATGGSSGAGGSGSGSVVASGSTGIQQDLRDLPFDMPKLRRRKTLQQEAACTSGSATSVDLGELPFDMPKLRRRLRANQAEINNLLMHSTESSGISQASSSHSMRDDQKMSSKLDTALFRQNLTLNLNESRQATKQFGSLDLRGLNSNKELNMNLSQGYVTAVDLIDVTIPLERQGWYHGAITRIEAETTLRPLSEGSFLVRNCESTKQDYSLSLKGAKGFMHMRIQRNETGQYILGQFSRPFETVPEMIRHFCLNRLPVRGAEHMCLIEPVIAQLL

Target ORF information:

RefSeq Version	NM_001272555.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heavyweight (hwt), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272555.2

ORF Insert Sequence:

ATGGAGCGCA TGTGGCGAAA AGTAAACCAC AGTTTGCGCA ACACCAGCAA GTCGCAAGCA 
CAGCAAGCAG CAGGTGGCGA TGGAATCATC GGTGGCCCAG CCACAGGAGC CACTGCAGCC 
ACGCCTCAAT CCACCGACAC GATTAGTTCG GCTGGCGGTT TCGGCGATGG CCAGCTGGTG 
GTCGCTGTCC AGGGATTAGC CAGCGGAGGC ATTGGTGCTT CCATCACTGC CAGTGGTGGC 
AGCAATGCCA CCGGACGACG ACTGGCCAGC AGCGGTGGAG GTGGCGGTTG TAACAAATCC 
CTTAGCCAAC ATGTCCTGCA AACACGTACC GCTTCCTCGG AACGCCGCAG GCGACGCCGT 
CGGAAAAGTC GCCCACGACG TGGCGGTGGA ATGGGTGTCA CCAGTTGCGT CGGCGATCCT 
GGTGGCGACA ATATGTCTTC CTCGGGCGGC TTTTATACAC TGAAAGAGCA CCAGCACTAT 
CGTCCCAGTC ACCATCATGG CGGGGGCGTT TCTGTCGGCG TTGGCGGTGG CTCATCCCGT 
CGTCTTTTCG CCACTTCGGC GCCCAGCGGC ACAGTGATGA TGTATCCGAA TCCCCAGCGC 
GGCACGGCAC TAGCACCATC TGTGGGATCT GGAACCGGGC ACGCAAACAC TGCGGGCTTA 
GGAGGAGGAG GAGGAGGAGG AGCCGGAGGT GCTGGAGGTG GAGCGACTGG ATCCGGAACT 
GGAGGACAGC CGGACATTTC ACTGAGACAG CGGGCGGTGG CCAAGTTGCG CATGTTCAAC 
TTCCATCTCA ACTGGGATCT GCACATGACG CACTGCAAGC CCTGCGGTCC CCGTTTGAGT 
GGCAGTGGTG GCAACATAAT CACCCGAAGG CTGTGCCGCA ATCGTCGGCG GGAGGACAAC 
GAACTGTACC GCTCCAATAG CTTCAAGTTC GAGCGATTCG AGCGCAAGGA GTGCCTGGAG 
GAGCTGTCCA ACACGTTGCA GAAGCAGATT GCCATCTGTG ATGATTACAG CCTGCCCGTA 
GACTTTGTTA AAAAACGACC GTCCAGCTTT GATCCGTGCC TGGCGGAGGC GATACCAGCG 
AATCCCGAGC ATTGGATAGC ACCCAGTCCG CAAACCGAGT TTCTGCCATT TGGTGAAGCC 
CAACAAGCGT TGCAGCTACA ACCAGCAGCA GCAGCAGCAA CAGCAGCAGC AACAACATCT 
GCAGCAGCAG CGGCAAACGC AACAGCAGCA GCTCCTCAAC TACCGCCACC GCTGCCCGTT 
CCCGTGGCAA CATTGCCCAA TCAGGCGGCG ACGGCAGCAG CAGCAGCAAC AGCTGCGATT 
TTGGCCACTC ACCAGCAACA GCAGCAGCAG CAGCATCAGC ATTTGAGCAA CAACAACAAC 
AACGGCGGCA GCACACACGG CCAACAGCAT ACGCACAGCA ACAATAACAA CACATTGCCA 
GTAGGGGCAC AGCAGCAGCA ACAGCAGCAG CATCAACAAC AACGTGCAAC ATTGCAACAT 
CCACCGCTGC CCAACAGCAA CATCAAGCAG GCATCGGTGA AATTAATCGA AGGATATTCA 
GATCCAAAGG ATACGCGCAA TCACAAAAAG GTCTATCACA AAAAGGCCAA GAAACGCTCG 
ACTACTGGCC ACAAGCGGCA TCAGCAACAG CAGCAGCAAC AGCAGCCGCA TCAGCAGCAA 
TTGCAGCAGC AGCAACAGCA AACGTTGCCA ACGCCTAGAA GTGCGCAGCA GCAACAGCAA 
CTGCAGCAAA AGTTACAGCA GCGGCATTCC ACCTACAACA ACAACAACGG CAACTCAAGA 
AAGGATAAAC GCAACAGTCA ATACAATTCA GACTCCTCGG CTCCGAAATC CTCCGACGAC 
GAGGCGGACA TCGACGAAGA GGTTGAGCAG CAACCGCTGC CCGTGCCACA AGCACCTGTC 
CAGAGTGCTT CACAACCAGC GGCCGGACTC AACTCGCCGG ATTCCATATC CGATGACCTG 
GTGCGTCCGC TTCCACCGCA ACTGCAGCGC AGTCCGCGCA AACTGACCGT CGCTCCGGCG 
GAGCACAACA AGCGCCAGCC CTCGCCCTAC TACTACTCCG ATCTGCTCAA GAGTCGTGAC 
AAGGACAAGG ATAAAGACAA GGACAAGGAC AAAGACAAGG ACAAGGATAA AGACAAGGAC 
AAGGACAAGG ACAAGGAACG GGACAAGGAC AGGGAGGACT TTAAGCTCAA GTGTCGCCAG 
TCGACGGCCA GTGAACCGCC GCAGCAAACC CAGACACAGT TGGGTGGCTA CCGGAAGAGC 
TCCAGCTTGG ATGTGCCGCC GGCGGAGGAG CAGCCGGAAG CAGAGAGTCA CGAAGAGGAG 
GATCCCCGGA CGGGACAGGA CTCTCAATCC ACGCCCAAGC GTTACAGTTT CACCGAGGAG 
GGTGTACACA TCATTCGCTG CGAGACGCCC AGCACCAGTA CTTCCGAGGA ATCCGATTGC 
AGTGAGTGCC TCAAGCGACG CGAGTGGCAC GCTCGTGCCT TAGCCTTAGT CCGCAAGACC 
TGCAACGTTC AGCCAAGGAA TCAGAATCCC ACTGGCAGCG AGCTTCAGCT GCAGTTGCAG 
CATTGCGACG CCGGCGAGGG CGAACTGCTT AAGTGCTGTC CACCGCCACC GGCTCCAGCG 
GAGAACCAGT CTCAGGTCCA GCCGATGCCA CCGCACCGCC TGCTGGGACC AGCAGCCGTG 
TGCGGAGCCT GCATCACATC CGCTCCTGCC TCCTCCTTGG CCGGCGGACG GGGCAGCGAG 
CTCGGCGATG ATCTGGAAGA GGAGTACTTC CGGCCGCGCA GCATCTTCTA TGTGCATCCA 
CATGGCGTCC ACGAGTGCAT CGAGTGCGCC CAGCCAGCCC AGCCTGCGAC TGTTCCAGCG 
CCAACGCTGA GCAATCCATC GCTCGATATC TACGGCGACG ACGAGGACGA AGTGGACGGT 
GACGAGGACG AGGACGAAGA GGACGACTCC GAGGACATCA TGGGCAGCCG GCGGCGGCAG 
ATCTATGAGA CGGCCTTCGA CTGCAAGATA GCCAAGTCGG ATGACGATCT AGATGAGGTG 
GATCGCATTA CCAATTGCTC GGTCTTGCTG CAGTTGAGTA ATGGCAACGG TGGTGACATC 
AGCAAGGTAA CCGGCTCTTC GAAGACGCGG GCCGAGTGCA AGCGAGCCAA AAACTCCAAA 
AATCAGGCTC TGCAAGAGCA AACGGGCGAG GCCCATGTAC AAGTGCATCA TGTGGAGGCG 
GAGCTGGGCA AGCTGCAGCT GAGTCAAGTG GACCAGCAGA ATCAGAATCA GAACCAGAGC 
CAAAACCAGC AGCATGGTGT GAGTGCTACC GGTAGTGGAG CAAGTGCATC CAGCACGCAG 
CAGCTGCCCG TGCGGGGATA TACCCCATCG CCGCCATCGA CGGCTCCGCT GCCGATGAAA 
TTTCCCGGCA AACATGATCG CTACCTGAAC ATGAACAGCA TCAGGAGTGC ACCCAATCTG 
CCCGCCGCCA ATCCAGCGCA TCCCCGTCTC CGGGATCTCC GTCTGCCCAT CCAGTCCATG 
CGGCATCAGT GCGGCGGCAG CAGCAATGAC AACAGTCTCA CCGACAGCGC CTACGTGAAT 
CCGCCCTCGA CCAACTCGAA TTCGAATGCG GCATCGGCTT CCGCCTCGGC CTCCGTCTCG 
GCAACCGGAT CAGCAGCTCC ACGCCGACCG CGATCTTTTG TCCTCGAATC GGGCCGGGTG 
CTAGAGCTGC GACGCAGTGC CCAGGGCCAT CAGGGTGGCC AGTCGACCCA CCATCACCAT 
CATCATCATC ATCATGCGCC GGGGAGCAGA AGAAATCGCC ATGGCCAAAG CCAGGGGCAA 
ACGACCGGTG GTGGCCACAA CTACAGCAGC ACCGAGAGCA TTGCCACCTC GAGCAGTGGC 
GGCAGCATGG AATCGCTGCG TTCCAGCACC AGCGAGGGCA ATCGCAGCAC CTCCAGCTCC 
GAGTCGCGGC ACTCCAGTTC GCTGAGCTCT CACAGCTCGG AGAGCGGCAG CGGTGGCAGC 
GGCGGTAGCG GAGGCTCCAG TGTGGCCTAT CCCCTGCGAC CGGCTCCCCT GCTGCTACAC 
TCCAAGCTGC ACATCCTGAG TCCCATTTCC GACAAGTCCT CGCAGGAACC GGCATCGGCC 
AGTGCCTCGG AGCAATCACA GCAGCCGTCC CAGCAGCTGG CGCCGATGGC GTCGCAGGAA 
GACGATTCCA CAACGAGTGG CGCACCAGCA GGGAACCAAG TCCAGGTGAA TGCCTCCTTG 
AAGCAGCCGC GTAGCAATCG GGGTGCACCA AATAAGGCCC TGCTCCAGCT GGCCGATGAG 
CTACTGGGTT CGGATAGTGG GATATCGCTG CATTCCCGGG AGGATGGAAA ACCGCAGGCC 
TTAGCGCTAC AGCGTTTAAC GCTGCCCAAG CTACAGTTGA TTGGTGCTGA AGGAACAACA 
ACGTCGGCAA CGGCCTCCGG AGCAGGAGGT AGCTCAGCAA CGGGTGGCAG TAGCGGCGCT 
GGAGGATCAG GATCCGGTTC GGTAGTTGCC AGCGGATCGA CTGGCATACA GCAGGATCTG 
CGCGATTTGC CCTTCGATAT GCCCAAACTG AGGCGACGCA AGACGCTGCA ACAGGAGGCC 
GCCTGCACAT CGGGCAGTGC CACGTCCGTC GATCTCGGCG AGCTGCCCTT CGACATGCCC 
AAGTTGCGAC GACGCCTGAG GGCCAACCAG GCGGAGATCA ATAACCTGCT GATGCACAGC 
ACCGAATCCA GTGGCATCTC GCAGGCTTCC TCCAGTCATT CGATGCGCGA CGACCAAAAG 
ATGTCCAGCA AGTTGGATAC GGCACTTTTT CGGCAGAATC TCACTCTAAA CCTTAATGAA 
TCGCGACAGG CGACCAAACA GTTTGGCAGC CTGGATCTGC GTGGTCTCAA TAGCAACAAG 
GAGCTAAACA TGAATCTCAG CCAGGGATAT GTCACTGCTG TGGATCTAAT CGATGTGACA 
ATTCCGCTGG AGCGGCAGGG TTGGTACCAT GGTGCCATAA CGCGAATTGA GGCGGAGACC 
ACACTGCGTC CGCTGTCCGA GGGATCCTTC CTGGTGCGCA ACTGCGAGTC CACGAAGCAG 
GACTATTCGC TATCCCTCAA GGGCGCCAAG GGCTTTATGC ACATGCGCAT CCAGCGCAAC 
GAGACTGGAC AGTACATCCT TGGCCAGTTT AGTCGTCCCT TCGAGACGGT ACCGGAGATG 
ATCCGGCACT TTTGCCTCAA CCGGCTGCCA GTGCGCGGTG CCGAGCACAT GTGTCTGATC 
GAGCCGGTCA TCGCCCAGCT GCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hwt ( NM_001298227.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285156.1 Drosophila melanogaster heavyweight (hwt), transcript variant D, mRNA.

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CloneID	OFa21443
Clone ID Related Accession (Same CDS sequence)	NM_001298227.1
Accession Version	NM_001298227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heavyweight, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATCAGTGAAA ACGAGGAAAG TGTGCTAGAG ATTGCCATCT GTGATGATTA CAGCCTGCCC 
GTAGACTTTG TTAAAAAACG ACCGTCCAGC TTTGATCCGT GCCTGGCGGA GGCGATACCA 
GCGAATCCCG AGCATTGGAT AGCACCCAGT CCGCAAACCG AGTTTCTGCC ATTTGGTGAA 
GCCCAACAAG CGTTGCAGCT ACAACCAGCA GCAGCAGCAG CAACAGCAGC AGCAACAACA 
TCTGCAGCAG CAGCGGCAAA CGCAACAGCA GCAGCTCCTC AACTACCGCC ACCGCTGCCC 
GTTCCCGTGG CAACATTGCC CAATCAGGCG GCGACGGCAG CAGCAGCAGC AACAGCTGCG 
ATTTTGGCCA CTCACCAGCA ACAGCAGCAG CAGCAGCATC AGCATTTGAG CAACAACAAC 
AACAACGGCG GCAGCACACA CGGCCAACAG CATACGCACA GCAACAATAA CAACACATTG 
CCAGTAGGGG CACAGCAGCA GCAACAGCAG CAGCATCAAC AACAACGTGC AACATTGCAA 
CATCCACCGC TGCCCAACAG CAACATCAAG CAGGCATCGG TGAAATTAAT CGAAGGATAT 
TCAGATCCAA AGGATACGCG CAATCACAAA AAGGTCTATC ACAAAAAGGC CAAGAAACGC 
TCGACTACTG GCCACAAGCG GCATCAGCAA CAGCAGCAGC AACAGCAGCC GCATCAGCAG 
CAATTGCAGC AGCAGCAACA GCAAACGTTG CCAACGCCTA GAAGTGCGCA GCAGCAACAG 
CAACTGCAGC AAAAGTTACA GCAGCGGCAT TCCACCTACA ACAACAACAA CGGCAACTCA 
AGAAAGGATA AACGCAACAG TCAATACAAT TCAGACTCCT CGGCTCCGAA ATCCTCCGAC 
GACGAGGCGG ACATCGACGA AGAGGTTGAG CAGCAACCGC TGCCCGTGCC ACAAGCACCT 
GTCCAGAGTG CTTCACAACC AGCGGCCGGA CTCAACTCGC CGGATTCCAT ATCCGATGAC 
CTGGTGCGTC CGCTTCCACC GCAACTGCAG CGCAGTCCGC GCAAACTGAC CGTCGCTCCG 
GCGGAGCACA ACAAGCGCCA GCCCTCGCCC TACTACTACT CCGATCTGCT CAAGAGTCGT 
GACAAGGACA AGGATAAAGA CAAGGACAAG GACAAAGACA AGGACAAGGA TAAAGACAAG 
GACAAGGACA AGGACAAGGA ACGGGACAAG GACAGGGAGG ACTTTAAGCT CAAGTGTCGC 
CAGTCGACGG CCAGTGAACC GCCGCAGCAA ACCCAGACAC AGTTGGGTGG CTACCGGAAG 
AGCTCCAGCT TGGATGTGCC GCCGGCGGAG GAGCAGCCGG AAGCAGAGAG TCACGAAGAG 
GAGGATCCCC GGACGGGACA GGACTCTCAA TCCACGCCCA AGCGTTACAG TTTCACCGAG 
GAGGGTGTAC ACATCATTCG CTGCGAGACG CCCAGCACCA GTACTTCCGA GGAATCCGAT 
TGCAGTGAGT GCCTCAAGCG ACGCGAGTGG CACGCTCGTG CCTTAGCCTT AGTCCGCAAG 
ACCTGCAACG TTCAGCCAAG GAATCAGAAT CCCACTGGCA GCGAGCTTCA GCTGCAGTTG 
CAGCATTGCG ACGCCGGCGA GGGCGAACTG CTTAAGTGCT GTCCACCGCC ACCGGCTCCA 
GCGGAGAACC AGTCTCAGGT CCAGCCGATG CCACCGCACC GCCTGCTGGG ACCAGCAGCC 
GTGTGCGGAG CCTGCATCAC ATCCGCTCCT GCCTCCTCCT TGGCCGGCGG ACGGGGCAGC 
GAGCTCGGCG ATGATCTGGA AGAGGAGTAC TTCCGGCCGC GCAGCATCTT CTATGTGCAT 
CCACATGGCG TCCACGAGTG CATCGAGTGC GCCCAGCCAG CCCAGCCTGC GACTGTTCCA 
GCGCCAACGC TGAGCAATCC ATCGCTCGAT ATCTACGGCG ACGACGAGGA CGAAGTGGAC 
GGTGACGAGG ACGAGGACGA AGAGGACGAC TCCGAGGACA TCATGGGCAG CCGGCGGCGG 
CAGATCTATG AGACGGCCTT CGACTGCAAG ATAGCCAAGT CGGATGACGA TCTAGATGAG 
GTGGATCGCA TTACCAATTG CTCGGTCTTG CTGCAGTTGA GTAATGGCAA CGGTGGTGAC 
ATCAGCAAGG TAACCGGCTC TTCGAAGACG CGGGCCGAGT GCAAGCGAGC CAAAAACTCC 
AAAAATCAGG CTCTGCAAGA GCAAACGGGC GAGGCCCATG TACAAGTGCA TCATGTGGAG 
GCGGAGCTGG GCAAGCTGCA GCTGAGTCAA GTGGACCAGC AGAATCAGAA TCAGAACCAG 
AGCCAAAACC AGCAGCATGG TGTGAGTGCT ACCGGTAGTG GAGCAAGTGC ATCCAGCACG 
CAGCAGCTGC CCGTGCGGGG ATATACCCCA TCGCCGCCAT CGACGGCTCC GCTGCCGATG 
AAATTTCCCG GCAAACATGA TCGCTACCTG AACATGAACA GCATCAGGAG TGCACCCAAT 
CTGCCCGCCG CCAATCCAGC GCATCCCCGT CTCCGGGATC TCCGTCTGCC CATCCAGTCC 
ATGCGGCATC AGTGCGGCGG CAGCAGCAAT GACAACAGTC TCACCGACAG CGCCTACGTG 
AATCCGCCCT CGACCAACTC GAATTCGAAT GCGGCATCGG CTTCCGCCTC GGCCTCCGTC 
TCGGCAACCG GATCAGCAGC TCCACGCCGA CCGCGATCTT TTGTCCTCGA ATCGGGCCGG 
GTGCTAGAGC TGCGACGCAG TGCCCAGGGC CATCAGGGTG GCCAGTCGAC CCACCATCAC 
CATCATCATC ATCATCATGC GCCGGGGAGC AGAAGAAATC GCCATGGCCA AAGCCAGGGG 
CAAACGACCG GTGGTGGCCA CAACTACAGC AGCACCGAGA GCATTGCCAC CTCGAGCAGT 
GGCGGCAGCA TGGAATCGCT GCGTTCCAGC ACCAGCGAGG GCAATCGCAG CACCTCCAGC 
TCCGAGTCGC GGCACTCCAG TTCGCTGAGC TCTCACAGCT CGGAGAGCGG CAGCGGTGGC 
AGCGGCGGTA GCGGAGGCTC CAGTGTGGCC TATCCCCTGC GACCGGCTCC CCTGCTGCTA 
CACTCCAAGC TGCACATCCT GAGTCCCATT TCCGACAAGT CCTCGCAGGA ACCGGCATCG 
GCCAGTGCCT CGGAGCAATC ACAGCAGCCG TCCCAGCAGC TGGCGCCGAT GGCGTCGCAG 
GAAGACGATT CCACAACGAG TGGCGCACCA GCAGGGAACC AAGTCCAGGT GAATGCCTCC 
TTGAAGCAGC CGCGTAGCAA TCGGGGTGCA CCAAATAAGG CCCTGCTCCA GCTGGCCGAT 
GAGCTACTGG GTTCGGATAG TGGGATATCG CTGCATTCCC GGGAGGATGG AAAACCGCAG 
GCCTTAGCGC TACAGCGTTT AACGCTGCCC AAGCTACAGT TGATTGGTGC TGAAGGAACA 
ACAACGTCGG CAACGGCCTC CGGAGCAGGA GGTAGCTCAG CAACGGGTGG CAGTAGCGGC 
GCTGGAGGAT CAGGATCCGG TTCGGTAGTT GCCAGCGGAT CGACTGGCAT ACAGCAGGAT 
CTGCGCGATT TGCCCTTCGA TATGCCCAAA CTGAGGCGAC GCAAGACGCT GCAACAGGAG 
GCCGCCTGCA CATCGGGCAG TGCCACGTCC GTCGATCTCG GCGAGCTGCC CTTCGACATG 
CCCAAGTTGC GACGACGCCT GAGGGCCAAC CAGGCGGAGA TCAATAACCT GCTGATGCAC 
AGCACCGAAT CCAGTGGCAT CTCGCAGGCT TCCTCCAGTC ATTCGATGCG CGACGACCAA 
AAGATGTCCA GCAAGTTGGA TACGGCACTT TTTCGGCAGA ATCTCACTCT AAACCTTAAT 
GAATCGCGAC AGGCGACCAA ACAGTTTGGC AGCCTGGATC TGCGTGGTCT CAATAGCAAC 
AAGGAGCTAA ACATGAATCT CAGCCAGGGA TATGTCACTG CTGTGGATCT AATCGATGTG 
ACAATTCCGC TGGAGCGGCA GGGTTGGTAC CATGGTGCCA TAACGCGAAT TGAGGCGGAG 
ACCACACTGC GTCCGCTGTC CGAGGGATCC TTCCTGGTGC GCAACTGCGA GTCCACGAAG 
CAGGACTATT CGCTATCCCT CAAGGGCGCC AAGGGCTTTA TGCACATGCG CATCCAGCGC 
AACGAGACTG GACAGTACAT CCTTGGCCAG TTTAGTCGTC CCTTCGAGAC GGTACCGGAG 
ATGATCCGGC ACTTTTGCCT CAACCGGCTG CCAGTGCGCG GTGCCGAGCA CATGTGTCTG 
ATCGAGCCGG TCATCGCCCA GCTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285156.1
CDS	292..4641
Translation	ISENEESVLEIAICDDYSLPVDFVKKRPSSFDPCLAEAIPANPEHWIAPSPQTEFLPFGEAQQALQLQPAAAAATAAATTSAAAAANATAAAPQLPPPLPVPVATLPNQAATAAAAATAAILATHQQQQQQQHQHLSNNNNNGGSTHGQQHTHSNNNNTLPVGAQQQQQQQHQQQRATLQHPPLPNSNIKQASVKLIEGYSDPKDTRNHKKVYHKKAKKRSTTGHKRHQQQQQQQQPHQQQLQQQQQQTLPTPRSAQQQQQLQQKLQQRHSTYNNNNGNSRKDKRNSQYNSDSSAPKSSDDEADIDEEVEQQPLPVPQAPVQSASQPAAGLNSPDSISDDLVRPLPPQLQRSPRKLTVAPAEHNKRQPSPYYYSDLLKSRDKDKDKDKDKDKDKDKDKDKDKDKDKERDKDREDFKLKCRQSTASEPPQQTQTQLGGYRKSSSLDVPPAEEQPEAESHEEEDPRTGQDSQSTPKRYSFTEEGVHIIRCETPSTSTSEESDCSECLKRREWHARALALVRKTCNVQPRNQNPTGSELQLQLQHCDAGEGELLKCCPPPPAPAENQSQVQPMPPHRLLGPAAVCGACITSAPASSLAGGRGSELGDDLEEEYFRPRSIFYVHPHGVHECIECAQPAQPATVPAPTLSNPSLDIYGDDEDEVDGDEDEDEEDDSEDIMGSRRRQIYETAFDCKIAKSDDDLDEVDRITNCSVLLQLSNGNGGDISKVTGSSKTRAECKRAKNSKNQALQEQTGEAHVQVHHVEAELGKLQLSQVDQQNQNQNQSQNQQHGVSATGSGASASSTQQLPVRGYTPSPPSTAPLPMKFPGKHDRYLNMNSIRSAPNLPAANPAHPRLRDLRLPIQSMRHQCGGSSNDNSLTDSAYVNPPSTNSNSNAASASASASVSATGSAAPRRPRSFVLESGRVLELRRSAQGHQGGQSTHHHHHHHHHAPGSRRNRHGQSQGQTTGGGHNYSSTESIATSSSGGSMESLRSSTSEGNRSTSSSESRHSSSLSSHSSESGSGGSGGSGGSSVAYPLRPAPLLLHSKLHILSPISDKSSQEPASASASEQSQQPSQQLAPMASQEDDSTTSGAPAGNQVQVNASLKQPRSNRGAPNKALLQLADELLGSDSGISLHSREDGKPQALALQRLTLPKLQLIGAEGTTTSATASGAGGSSATGGSSGAGGSGSGSVVASGSTGIQQDLRDLPFDMPKLRRRKTLQQEAACTSGSATSVDLGELPFDMPKLRRRLRANQAEINNLLMHSTESSGISQASSSHSMRDDQKMSSKLDTALFRQNLTLNLNESRQATKQFGSLDLRGLNSNKELNMNLSQGYVTAVDLIDVTIPLERQGWYHGAITRIEAETTLRPLSEGSFLVRNCESTKQDYSLSLKGAKGFMHMRIQRNETGQYILGQFSRPFETVPEMIRHFCLNRLPVRGAEHMCLIEPVIAQLL

Target ORF information:

RefSeq Version	NM_001298227.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heavyweight (hwt), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298227.1

ORF Insert Sequence:

ATCAGTGAAA ACGAGGAAAG TGTGCTAGAG ATTGCCATCT GTGATGATTA CAGCCTGCCC 
GTAGACTTTG TTAAAAAACG ACCGTCCAGC TTTGATCCGT GCCTGGCGGA GGCGATACCA 
GCGAATCCCG AGCATTGGAT AGCACCCAGT CCGCAAACCG AGTTTCTGCC ATTTGGTGAA 
GCCCAACAAG CGTTGCAGCT ACAACCAGCA GCAGCAGCAG CAACAGCAGC AGCAACAACA 
TCTGCAGCAG CAGCGGCAAA CGCAACAGCA GCAGCTCCTC AACTACCGCC ACCGCTGCCC 
GTTCCCGTGG CAACATTGCC CAATCAGGCG GCGACGGCAG CAGCAGCAGC AACAGCTGCG 
ATTTTGGCCA CTCACCAGCA ACAGCAGCAG CAGCAGCATC AGCATTTGAG CAACAACAAC 
AACAACGGCG GCAGCACACA CGGCCAACAG CATACGCACA GCAACAATAA CAACACATTG 
CCAGTAGGGG CACAGCAGCA GCAACAGCAG CAGCATCAAC AACAACGTGC AACATTGCAA 
CATCCACCGC TGCCCAACAG CAACATCAAG CAGGCATCGG TGAAATTAAT CGAAGGATAT 
TCAGATCCAA AGGATACGCG CAATCACAAA AAGGTCTATC ACAAAAAGGC CAAGAAACGC 
TCGACTACTG GCCACAAGCG GCATCAGCAA CAGCAGCAGC AACAGCAGCC GCATCAGCAG 
CAATTGCAGC AGCAGCAACA GCAAACGTTG CCAACGCCTA GAAGTGCGCA GCAGCAACAG 
CAACTGCAGC AAAAGTTACA GCAGCGGCAT TCCACCTACA ACAACAACAA CGGCAACTCA 
AGAAAGGATA AACGCAACAG TCAATACAAT TCAGACTCCT CGGCTCCGAA ATCCTCCGAC 
GACGAGGCGG ACATCGACGA AGAGGTTGAG CAGCAACCGC TGCCCGTGCC ACAAGCACCT 
GTCCAGAGTG CTTCACAACC AGCGGCCGGA CTCAACTCGC CGGATTCCAT ATCCGATGAC 
CTGGTGCGTC CGCTTCCACC GCAACTGCAG CGCAGTCCGC GCAAACTGAC CGTCGCTCCG 
GCGGAGCACA ACAAGCGCCA GCCCTCGCCC TACTACTACT CCGATCTGCT CAAGAGTCGT 
GACAAGGACA AGGATAAAGA CAAGGACAAG GACAAAGACA AGGACAAGGA TAAAGACAAG 
GACAAGGACA AGGACAAGGA ACGGGACAAG GACAGGGAGG ACTTTAAGCT CAAGTGTCGC 
CAGTCGACGG CCAGTGAACC GCCGCAGCAA ACCCAGACAC AGTTGGGTGG CTACCGGAAG 
AGCTCCAGCT TGGATGTGCC GCCGGCGGAG GAGCAGCCGG AAGCAGAGAG TCACGAAGAG 
GAGGATCCCC GGACGGGACA GGACTCTCAA TCCACGCCCA AGCGTTACAG TTTCACCGAG 
GAGGGTGTAC ACATCATTCG CTGCGAGACG CCCAGCACCA GTACTTCCGA GGAATCCGAT 
TGCAGTGAGT GCCTCAAGCG ACGCGAGTGG CACGCTCGTG CCTTAGCCTT AGTCCGCAAG 
ACCTGCAACG TTCAGCCAAG GAATCAGAAT CCCACTGGCA GCGAGCTTCA GCTGCAGTTG 
CAGCATTGCG ACGCCGGCGA GGGCGAACTG CTTAAGTGCT GTCCACCGCC ACCGGCTCCA 
GCGGAGAACC AGTCTCAGGT CCAGCCGATG CCACCGCACC GCCTGCTGGG ACCAGCAGCC 
GTGTGCGGAG CCTGCATCAC ATCCGCTCCT GCCTCCTCCT TGGCCGGCGG ACGGGGCAGC 
GAGCTCGGCG ATGATCTGGA AGAGGAGTAC TTCCGGCCGC GCAGCATCTT CTATGTGCAT 
CCACATGGCG TCCACGAGTG CATCGAGTGC GCCCAGCCAG CCCAGCCTGC GACTGTTCCA 
GCGCCAACGC TGAGCAATCC ATCGCTCGAT ATCTACGGCG ACGACGAGGA CGAAGTGGAC 
GGTGACGAGG ACGAGGACGA AGAGGACGAC TCCGAGGACA TCATGGGCAG CCGGCGGCGG 
CAGATCTATG AGACGGCCTT CGACTGCAAG ATAGCCAAGT CGGATGACGA TCTAGATGAG 
GTGGATCGCA TTACCAATTG CTCGGTCTTG CTGCAGTTGA GTAATGGCAA CGGTGGTGAC 
ATCAGCAAGG TAACCGGCTC TTCGAAGACG CGGGCCGAGT GCAAGCGAGC CAAAAACTCC 
AAAAATCAGG CTCTGCAAGA GCAAACGGGC GAGGCCCATG TACAAGTGCA TCATGTGGAG 
GCGGAGCTGG GCAAGCTGCA GCTGAGTCAA GTGGACCAGC AGAATCAGAA TCAGAACCAG 
AGCCAAAACC AGCAGCATGG TGTGAGTGCT ACCGGTAGTG GAGCAAGTGC ATCCAGCACG 
CAGCAGCTGC CCGTGCGGGG ATATACCCCA TCGCCGCCAT CGACGGCTCC GCTGCCGATG 
AAATTTCCCG GCAAACATGA TCGCTACCTG AACATGAACA GCATCAGGAG TGCACCCAAT 
CTGCCCGCCG CCAATCCAGC GCATCCCCGT CTCCGGGATC TCCGTCTGCC CATCCAGTCC 
ATGCGGCATC AGTGCGGCGG CAGCAGCAAT GACAACAGTC TCACCGACAG CGCCTACGTG 
AATCCGCCCT CGACCAACTC GAATTCGAAT GCGGCATCGG CTTCCGCCTC GGCCTCCGTC 
TCGGCAACCG GATCAGCAGC TCCACGCCGA CCGCGATCTT TTGTCCTCGA ATCGGGCCGG 
GTGCTAGAGC TGCGACGCAG TGCCCAGGGC CATCAGGGTG GCCAGTCGAC CCACCATCAC 
CATCATCATC ATCATCATGC GCCGGGGAGC AGAAGAAATC GCCATGGCCA AAGCCAGGGG 
CAAACGACCG GTGGTGGCCA CAACTACAGC AGCACCGAGA GCATTGCCAC CTCGAGCAGT 
GGCGGCAGCA TGGAATCGCT GCGTTCCAGC ACCAGCGAGG GCAATCGCAG CACCTCCAGC 
TCCGAGTCGC GGCACTCCAG TTCGCTGAGC TCTCACAGCT CGGAGAGCGG CAGCGGTGGC 
AGCGGCGGTA GCGGAGGCTC CAGTGTGGCC TATCCCCTGC GACCGGCTCC CCTGCTGCTA 
CACTCCAAGC TGCACATCCT GAGTCCCATT TCCGACAAGT CCTCGCAGGA ACCGGCATCG 
GCCAGTGCCT CGGAGCAATC ACAGCAGCCG TCCCAGCAGC TGGCGCCGAT GGCGTCGCAG 
GAAGACGATT CCACAACGAG TGGCGCACCA GCAGGGAACC AAGTCCAGGT GAATGCCTCC 
TTGAAGCAGC CGCGTAGCAA TCGGGGTGCA CCAAATAAGG CCCTGCTCCA GCTGGCCGAT 
GAGCTACTGG GTTCGGATAG TGGGATATCG CTGCATTCCC GGGAGGATGG AAAACCGCAG 
GCCTTAGCGC TACAGCGTTT AACGCTGCCC AAGCTACAGT TGATTGGTGC TGAAGGAACA 
ACAACGTCGG CAACGGCCTC CGGAGCAGGA GGTAGCTCAG CAACGGGTGG CAGTAGCGGC 
GCTGGAGGAT CAGGATCCGG TTCGGTAGTT GCCAGCGGAT CGACTGGCAT ACAGCAGGAT 
CTGCGCGATT TGCCCTTCGA TATGCCCAAA CTGAGGCGAC GCAAGACGCT GCAACAGGAG 
GCCGCCTGCA CATCGGGCAG TGCCACGTCC GTCGATCTCG GCGAGCTGCC CTTCGACATG 
CCCAAGTTGC GACGACGCCT GAGGGCCAAC CAGGCGGAGA TCAATAACCT GCTGATGCAC 
AGCACCGAAT CCAGTGGCAT CTCGCAGGCT TCCTCCAGTC ATTCGATGCG CGACGACCAA 
AAGATGTCCA GCAAGTTGGA TACGGCACTT TTTCGGCAGA ATCTCACTCT AAACCTTAAT 
GAATCGCGAC AGGCGACCAA ACAGTTTGGC AGCCTGGATC TGCGTGGTCT CAATAGCAAC 
AAGGAGCTAA ACATGAATCT CAGCCAGGGA TATGTCACTG CTGTGGATCT AATCGATGTG 
ACAATTCCGC TGGAGCGGCA GGGTTGGTAC CATGGTGCCA TAACGCGAAT TGAGGCGGAG 
ACCACACTGC GTCCGCTGTC CGAGGGATCC TTCCTGGTGC GCAACTGCGA GTCCACGAAG 
CAGGACTATT CGCTATCCCT CAAGGGCGCC AAGGGCTTTA TGCACATGCG CATCCAGCGC 
AACGAGACTG GACAGTACAT CCTTGGCCAG TTTAGTCGTC CCTTCGAGAC GGTACCGGAG 
ATGATCCGGC ACTTTTGCCT CAACCGGCTG CCAGTGCGCG GTGCCGAGCA CATGTGTCTG 
ATCGAGCCGG TCATCGCCCA GCTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hwt cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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