Dif cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dif
Entrez Gene ID	35045
Full Name	Dorsal-related immunity factor
Synonyms	6794,CG6794,DIF,Dmel\CG6794,dif
General protein information	Preferred Names Dorsal-related immunity factor Names CG6794 gene product from transcript CG6794-RD CG6794-PA CG6794-PB CG6794-PC CG6794-PD Dif-PA Dif-PB Dif-PC Dif-PD Dorsal-like immunity factor Dorsal-related transcription factor dorsal related immunity factor dorsal-related immunity factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36C7-36C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_165216.3	NP_724050.1	Dorsal-related immunity factor, isoform B
NM_001169528.1	NP_001162999.1	Dorsal-related immunity factor, isoform D
NM_001169527.2	NP_001162998.1	Dorsal-related immunity factor, isoform C
NM_078865.4	NP_523589.2	Dorsal-related immunity factor, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results, not only revealed a novel function and modulation pattern of miR-958, but also provided a new insight into the underlying molecular mechanisms of Toll signaling in regulation of innate immunity.
Title: miR-958 inhibits Toll signaling and Drosomycin expression via direct targeting of Toll and Dif in Drosophila melanogaster.

Fasting increases survival to cold in dif mutant Drosophila melanogaster.
Title: Fasting increases survival to cold in FOXO, DIF, autophagy mutants and in other genotypes of Drosophila melanogaster.

DIF factor seems to be involved in the response to fungal infection after a mild cold stress and in the resistance to heat. DIF seems to have no role in the increased longevity of non-infected flies and resistance to a severe cold shock.
Title: The NF-?B-like factor DIF could explain some positive effects of a mild stress on longevity, behavioral aging, and resistance to strong stresses in Drosophila melanogaster.

Data show that the DIF and Relish complex is detectable in whole animal extracts, suggesting that this heterodimer may function in vivo to increase the spectrum and level of antimicrobial peptide production in response to different infections.
Title: Heterodimers of NF-kappaB transcription factors DIF and Relish regulate antimicrobial peptide genes in Drosophila.

Molecular modeling was used to compare the tertiary structure of Dif with two other Drosophila proteins.
Title: Functional conservation of Rel binding sites in drosophilid genomes.

The following Dif gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dif cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14819	NM_078865.4 Latest version!	Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14819	NM_165216.3 Latest version!	Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14819	NM_001169528.1 Latest version!	Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16875	NM_001169527.2 Latest version!	Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dif ( NM_165216.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724050.1 Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14819
Clone ID Related Accession (Same CDS sequence)	NM_165216.3 , NM_001169528.1 , NM_078865.4
Accession Version	NM_165216.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dorsal-related immunity factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165216.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGAGG AGGCTTTCGG CGATATACAG GAAATTATCA ATGCCAGCAT GGAGTTGAAT 
GGTGGCGCCA CCGGCGGAGG CAGTGTGGCA GGAGCTGTTG GCGGAGGCGG TGCTGCACAT 
CACATATTAT CGCAGTCGAC TTCCCTGCCG GTAATGCCGT CGCACATTCC GCTCCACCTG 
CAGAATCAGA ATATGAATCA GAATCTGCCC GAGCCAAGTG CAAGAAGTGG TCCCCACCTG 
CGTATCGTGG AGGAGCCGAC AAGCAATATA ATCCGCTTTC GCTACAAATG CGAGGGTCGC 
ACCGCCGGTT CGATTCCGGG CATGAACTCC AGCTCGGAAA CGGGCAAGAC CTTTCCCACC 
ATCGAAGTGT GCAACTACGA TGGACCCGTC ATCATCGTGG TCTCCTGTGT GACGAGCGAC 
GAGCCCTTCC GCCAGCATCC CCACTGGCTG GTCAGCAAGG AGGAGGCGGA TGCCTGCAAG 
TCGGGCATCT ACCAAAAGAA ATTGCCGCCA GAGGAGCGGC GGCTGGTGCT CCAAAAAGTG 
GGCATACAGT GCGCCAAGAA GCTGGAGATG CGCGACTCGC TGGTGGAGAG GGAAAGGAGA 
AACATCGATC CCTTCAATGC CAAATTCGAT CACAAGGACC AGATCGACAA GATCAATCGG 
TATGAGTTGC GCCTCTGCTA CCAGGCCTTC ATCACAGTGG GCAACTCAAA AGTGCCCCTG 
GATCCCATCG TATCCTCACC GATTTACGGC AAGAGCAGCG AACTGACCAT CACCCGGCTG 
TGCAGTTGCG CGGCCACTGC GAACGGCGGC GACGAGATCA TCATGCTGTG CGAGAAGATT 
GCCAAGGACG ACATCGAGGT GCGATTCTAT GAGACGGACA AGGATGGACG GGAGACGTGG 
TTCGCCAACG CAGAGTTCCA ACCCACGGAC GTGTTCAAAC AGATGGCCAT AGCTTTTAAG 
ACGCCGCGCT ACAGGAACAC CGAGATCACA CAAAGTGTTA ATGTGGAGCT GAAACTAGTG 
AGACCCTCGG ATGGAGCGAC GAGTGCCCCA CTGCCGTTCG AGTACTACCC GAATCCAGAA 
CTCCTAACCA AACACAATCG CCGTGTTGCC CAAAAAACAG TTGAAAGCCT AAAGCGCAGT 
CTCATGAGCA CCAATTTACA TCCATCCAAG CAGGTTAAAA CCTCCAGCCA GTATACGATA 
TTTTCCAAGC CACAAATTGC GACCACAACA CCGCAGACAC AGGTGTCGCC TGGTATGCCT 
TTGATGTTTC CCGGCGGAAG TCCCAATTTT GTGCAGGATA TCAAGATGGA GAATGGATTC 
ATGGATGTGG ATAGCCAGAG TAGCCAGTGT CCATCAGTGG AACGCAATTT CGCTTCGCCT 
CGATCCAACT GCAGTACAGT GGATAGTATT CCGCCGATGC AAATGGGGCA AAATCAAACT 
CATCTTTATT TGCCAGATGC CACAAACTTC ACATTTAATG GGAATTTCGC ATCGCCTTCG 
TCCAATTGCA GTACAGTGGA TAGCATTCCG CCGTTTCAAA TCGGACAGAG GAATAACCAT 
ATGTATCTGC CGGAGAATTC AAACTTTCCG GTAAACGGCT GTAGTCCTAC CCATTTCAGT 
GGTGGCTCTA TGACACCAAT CAATAATAAC AACAATGTAC TAATTAATAA CAATAACAAT 
GACTTTTTAA GTCAGAAAAT GAGTGCCATA AGCATACCGC CACAAGGAAA TTTTGGCATT 
AAACAGGTAT ATCAACAGAC TCAGCAGTTC CTGCCGCAAT TGCAGCCAGA GTCCATTCCA 
TATCTGGCCC AATCCCATCC AGAACAGTCG CAGTACCAAC AGCAACAGCA GCCACAGGAG 
CAGCAACCTC CAGCAGATGA ACCCACCCAA TCGTTTAGTG ATCTAATTTC CAGCAGCATA 
GGCATGGCGC CCATTGACAC CAGCGAACTT ATCCAGGACA TTGAGGCAGA ACTCAATAGC 
TTGGGAATTC AGCCATTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724050.1
CDS	213..2216
Translation	MFEEAFGDIQEIINASMELNGGATGGGSVAGAVGGGGAAHHILSQSTSLPVMPSHIPLHLQNQNMNQNLPEPSARSGPHLRIVEEPTSNIIRFRYKCEGRTAGSIPGMNSSSETGKTFPTIEVCNYDGPVIIVVSCVTSDEPFRQHPHWLVSKEEADACKSGIYQKKLPPEERRLVLQKVGIQCAKKLEMRDSLVERERRNIDPFNAKFDHKDQIDKINRYELRLCYQAFITVGNSKVPLDPIVSSPIYGKSSELTITRLCSCAATANGGDEIIMLCEKIAKDDIEVRFYETDKDGRETWFANAEFQPTDVFKQMAIAFKTPRYRNTEITQSVNVELKLVRPSDGATSAPLPFEYYPNPELLTKHNRRVAQKTVESLKRSLMSTNLHPSKQVKTSSQYTIFSKPQIATTTPQTQVSPGMPLMFPGGSPNFVQDIKMENGFMDVDSQSSQCPSVERNFASPRSNCSTVDSIPPMQMGQNQTHLYLPDATNFTFNGNFASPSSNCSTVDSIPPFQIGQRNNHMYLPENSNFPVNGCSPTHFSGGSMTPINNNNNVLINNNNNDFLSQKMSAISIPPQGNFGIKQVYQQTQQFLPQLQPESIPYLAQSHPEQSQYQQQQQPQEQQPPADEPTQSFSDLISSSIGMAPIDTSELIQDIEAELNSLGIQPFK

Target ORF information:

RefSeq Version	NM_165216.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165216.3

ORF Insert Sequence:

ATGTTTGAGG AGGCTTTCGG CGATATACAG GAAATTATCA ATGCCAGCAT GGAGTTGAAT 
GGTGGCGCCA CCGGCGGAGG CAGTGTGGCA GGAGCTGTTG GCGGAGGCGG TGCTGCACAT 
CACATATTAT CGCAGTCGAC TTCCCTGCCG GTAATGCCGT CGCACATTCC GCTCCACCTG 
CAGAATCAGA ATATGAATCA GAATCTGCCC GAGCCAAGTG CAAGAAGTGG TCCCCACCTG 
CGTATCGTGG AGGAGCCGAC AAGCAATATA ATCCGCTTTC GCTACAAATG CGAGGGTCGC 
ACCGCCGGTT CGATTCCGGG CATGAACTCC AGCTCGGAAA CGGGCAAGAC CTTTCCCACC 
ATCGAAGTGT GCAACTACGA TGGACCCGTC ATCATCGTGG TCTCCTGTGT GACGAGCGAC 
GAGCCCTTCC GCCAGCATCC CCACTGGCTG GTCAGCAAGG AGGAGGCGGA TGCCTGCAAG 
TCGGGCATCT ACCAAAAGAA ATTGCCGCCA GAGGAGCGGC GGCTGGTGCT CCAAAAAGTG 
GGCATACAGT GCGCCAAGAA GCTGGAGATG CGCGACTCGC TGGTGGAGAG GGAAAGGAGA 
AACATCGATC CCTTCAATGC CAAATTCGAT CACAAGGACC AGATCGACAA GATCAATCGG 
TATGAGTTGC GCCTCTGCTA CCAGGCCTTC ATCACAGTGG GCAACTCAAA AGTGCCCCTG 
GATCCCATCG TATCCTCACC GATTTACGGC AAGAGCAGCG AACTGACCAT CACCCGGCTG 
TGCAGTTGCG CGGCCACTGC GAACGGCGGC GACGAGATCA TCATGCTGTG CGAGAAGATT 
GCCAAGGACG ACATCGAGGT GCGATTCTAT GAGACGGACA AGGATGGACG GGAGACGTGG 
TTCGCCAACG CAGAGTTCCA ACCCACGGAC GTGTTCAAAC AGATGGCCAT AGCTTTTAAG 
ACGCCGCGCT ACAGGAACAC CGAGATCACA CAAAGTGTTA ATGTGGAGCT GAAACTAGTG 
AGACCCTCGG ATGGAGCGAC GAGTGCCCCA CTGCCGTTCG AGTACTACCC GAATCCAGAA 
CTCCTAACCA AACACAATCG CCGTGTTGCC CAAAAAACAG TTGAAAGCCT AAAGCGCAGT 
CTCATGAGCA CCAATTTACA TCCATCCAAG CAGGTTAAAA CCTCCAGCCA GTATACGATA 
TTTTCCAAGC CACAAATTGC GACCACAACA CCGCAGACAC AGGTGTCGCC TGGTATGCCT 
TTGATGTTTC CCGGCGGAAG TCCCAATTTT GTGCAGGATA TCAAGATGGA GAATGGATTC 
ATGGATGTGG ATAGCCAGAG TAGCCAGTGT CCATCAGTGG AACGCAATTT CGCTTCGCCT 
CGATCCAACT GCAGTACAGT GGATAGTATT CCGCCGATGC AAATGGGGCA AAATCAAACT 
CATCTTTATT TGCCAGATGC CACAAACTTC ACATTTAATG GGAATTTCGC ATCGCCTTCG 
TCCAATTGCA GTACAGTGGA TAGCATTCCG CCGTTTCAAA TCGGACAGAG GAATAACCAT 
ATGTATCTGC CGGAGAATTC AAACTTTCCG GTAAACGGCT GTAGTCCTAC CCATTTCAGT 
GGTGGCTCTA TGACACCAAT CAATAATAAC AACAATGTAC TAATTAATAA CAATAACAAT 
GACTTTTTAA GTCAGAAAAT GAGTGCCATA AGCATACCGC CACAAGGAAA TTTTGGCATT 
AAACAGGTAT ATCAACAGAC TCAGCAGTTC CTGCCGCAAT TGCAGCCAGA GTCCATTCCA 
TATCTGGCCC AATCCCATCC AGAACAGTCG CAGTACCAAC AGCAACAGCA GCCACAGGAG 
CAGCAACCTC CAGCAGATGA ACCCACCCAA TCGTTTAGTG ATCTAATTTC CAGCAGCATA 
GGCATGGCGC CCATTGACAC CAGCGAACTT ATCCAGGACA TTGAGGCAGA ACTCAATAGC 
TTGGGAATTC AGCCATTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dif ( NM_001169528.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162999.1 Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa14819
Clone ID Related Accession (Same CDS sequence)	NM_165216.3 , NM_001169528.1 , NM_078865.4
Accession Version	NM_001169528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dorsal-related immunity factor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGAGG AGGCTTTCGG CGATATACAG GAAATTATCA ATGCCAGCAT GGAGTTGAAT 
GGTGGCGCCA CCGGCGGAGG CAGTGTGGCA GGAGCTGTTG GCGGAGGCGG TGCTGCACAT 
CACATATTAT CGCAGTCGAC TTCCCTGCCG GTAATGCCGT CGCACATTCC GCTCCACCTG 
CAGAATCAGA ATATGAATCA GAATCTGCCC GAGCCAAGTG CAAGAAGTGG TCCCCACCTG 
CGTATCGTGG AGGAGCCGAC AAGCAATATA ATCCGCTTTC GCTACAAATG CGAGGGTCGC 
ACCGCCGGTT CGATTCCGGG CATGAACTCC AGCTCGGAAA CGGGCAAGAC CTTTCCCACC 
ATCGAAGTGT GCAACTACGA TGGACCCGTC ATCATCGTGG TCTCCTGTGT GACGAGCGAC 
GAGCCCTTCC GCCAGCATCC CCACTGGCTG GTCAGCAAGG AGGAGGCGGA TGCCTGCAAG 
TCGGGCATCT ACCAAAAGAA ATTGCCGCCA GAGGAGCGGC GGCTGGTGCT CCAAAAAGTG 
GGCATACAGT GCGCCAAGAA GCTGGAGATG CGCGACTCGC TGGTGGAGAG GGAAAGGAGA 
AACATCGATC CCTTCAATGC CAAATTCGAT CACAAGGACC AGATCGACAA GATCAATCGG 
TATGAGTTGC GCCTCTGCTA CCAGGCCTTC ATCACAGTGG GCAACTCAAA AGTGCCCCTG 
GATCCCATCG TATCCTCACC GATTTACGGC AAGAGCAGCG AACTGACCAT CACCCGGCTG 
TGCAGTTGCG CGGCCACTGC GAACGGCGGC GACGAGATCA TCATGCTGTG CGAGAAGATT 
GCCAAGGACG ACATCGAGGT GCGATTCTAT GAGACGGACA AGGATGGACG GGAGACGTGG 
TTCGCCAACG CAGAGTTCCA ACCCACGGAC GTGTTCAAAC AGATGGCCAT AGCTTTTAAG 
ACGCCGCGCT ACAGGAACAC CGAGATCACA CAAAGTGTTA ATGTGGAGCT GAAACTAGTG 
AGACCCTCGG ATGGAGCGAC GAGTGCCCCA CTGCCGTTCG AGTACTACCC GAATCCAGAA 
CTCCTAACCA AACACAATCG CCGTGTTGCC CAAAAAACAG TTGAAAGCCT AAAGCGCAGT 
CTCATGAGCA CCAATTTACA TCCATCCAAG CAGGTTAAAA CCTCCAGCCA GTATACGATA 
TTTTCCAAGC CACAAATTGC GACCACAACA CCGCAGACAC AGGTGTCGCC TGGTATGCCT 
TTGATGTTTC CCGGCGGAAG TCCCAATTTT GTGCAGGATA TCAAGATGGA GAATGGATTC 
ATGGATGTGG ATAGCCAGAG TAGCCAGTGT CCATCAGTGG AACGCAATTT CGCTTCGCCT 
CGATCCAACT GCAGTACAGT GGATAGTATT CCGCCGATGC AAATGGGGCA AAATCAAACT 
CATCTTTATT TGCCAGATGC CACAAACTTC ACATTTAATG GGAATTTCGC ATCGCCTTCG 
TCCAATTGCA GTACAGTGGA TAGCATTCCG CCGTTTCAAA TCGGACAGAG GAATAACCAT 
ATGTATCTGC CGGAGAATTC AAACTTTCCG GTAAACGGCT GTAGTCCTAC CCATTTCAGT 
GGTGGCTCTA TGACACCAAT CAATAATAAC AACAATGTAC TAATTAATAA CAATAACAAT 
GACTTTTTAA GTCAGAAAAT GAGTGCCATA AGCATACCGC CACAAGGAAA TTTTGGCATT 
AAACAGGTAT ATCAACAGAC TCAGCAGTTC CTGCCGCAAT TGCAGCCAGA GTCCATTCCA 
TATCTGGCCC AATCCCATCC AGAACAGTCG CAGTACCAAC AGCAACAGCA GCCACAGGAG 
CAGCAACCTC CAGCAGATGA ACCCACCCAA TCGTTTAGTG ATCTAATTTC CAGCAGCATA 
GGCATGGCGC CCATTGACAC CAGCGAACTT ATCCAGGACA TTGAGGCAGA ACTCAATAGC 
TTGGGAATTC AGCCATTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162999.1
CDS	325..2328
Translation	MFEEAFGDIQEIINASMELNGGATGGGSVAGAVGGGGAAHHILSQSTSLPVMPSHIPLHLQNQNMNQNLPEPSARSGPHLRIVEEPTSNIIRFRYKCEGRTAGSIPGMNSSSETGKTFPTIEVCNYDGPVIIVVSCVTSDEPFRQHPHWLVSKEEADACKSGIYQKKLPPEERRLVLQKVGIQCAKKLEMRDSLVERERRNIDPFNAKFDHKDQIDKINRYELRLCYQAFITVGNSKVPLDPIVSSPIYGKSSELTITRLCSCAATANGGDEIIMLCEKIAKDDIEVRFYETDKDGRETWFANAEFQPTDVFKQMAIAFKTPRYRNTEITQSVNVELKLVRPSDGATSAPLPFEYYPNPELLTKHNRRVAQKTVESLKRSLMSTNLHPSKQVKTSSQYTIFSKPQIATTTPQTQVSPGMPLMFPGGSPNFVQDIKMENGFMDVDSQSSQCPSVERNFASPRSNCSTVDSIPPMQMGQNQTHLYLPDATNFTFNGNFASPSSNCSTVDSIPPFQIGQRNNHMYLPENSNFPVNGCSPTHFSGGSMTPINNNNNVLINNNNNDFLSQKMSAISIPPQGNFGIKQVYQQTQQFLPQLQPESIPYLAQSHPEQSQYQQQQQPQEQQPPADEPTQSFSDLISSSIGMAPIDTSELIQDIEAELNSLGIQPFK

Target ORF information:

RefSeq Version	NM_001169528.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169528.1

ORF Insert Sequence:

ATGTTTGAGG AGGCTTTCGG CGATATACAG GAAATTATCA ATGCCAGCAT GGAGTTGAAT 
GGTGGCGCCA CCGGCGGAGG CAGTGTGGCA GGAGCTGTTG GCGGAGGCGG TGCTGCACAT 
CACATATTAT CGCAGTCGAC TTCCCTGCCG GTAATGCCGT CGCACATTCC GCTCCACCTG 
CAGAATCAGA ATATGAATCA GAATCTGCCC GAGCCAAGTG CAAGAAGTGG TCCCCACCTG 
CGTATCGTGG AGGAGCCGAC AAGCAATATA ATCCGCTTTC GCTACAAATG CGAGGGTCGC 
ACCGCCGGTT CGATTCCGGG CATGAACTCC AGCTCGGAAA CGGGCAAGAC CTTTCCCACC 
ATCGAAGTGT GCAACTACGA TGGACCCGTC ATCATCGTGG TCTCCTGTGT GACGAGCGAC 
GAGCCCTTCC GCCAGCATCC CCACTGGCTG GTCAGCAAGG AGGAGGCGGA TGCCTGCAAG 
TCGGGCATCT ACCAAAAGAA ATTGCCGCCA GAGGAGCGGC GGCTGGTGCT CCAAAAAGTG 
GGCATACAGT GCGCCAAGAA GCTGGAGATG CGCGACTCGC TGGTGGAGAG GGAAAGGAGA 
AACATCGATC CCTTCAATGC CAAATTCGAT CACAAGGACC AGATCGACAA GATCAATCGG 
TATGAGTTGC GCCTCTGCTA CCAGGCCTTC ATCACAGTGG GCAACTCAAA AGTGCCCCTG 
GATCCCATCG TATCCTCACC GATTTACGGC AAGAGCAGCG AACTGACCAT CACCCGGCTG 
TGCAGTTGCG CGGCCACTGC GAACGGCGGC GACGAGATCA TCATGCTGTG CGAGAAGATT 
GCCAAGGACG ACATCGAGGT GCGATTCTAT GAGACGGACA AGGATGGACG GGAGACGTGG 
TTCGCCAACG CAGAGTTCCA ACCCACGGAC GTGTTCAAAC AGATGGCCAT AGCTTTTAAG 
ACGCCGCGCT ACAGGAACAC CGAGATCACA CAAAGTGTTA ATGTGGAGCT GAAACTAGTG 
AGACCCTCGG ATGGAGCGAC GAGTGCCCCA CTGCCGTTCG AGTACTACCC GAATCCAGAA 
CTCCTAACCA AACACAATCG CCGTGTTGCC CAAAAAACAG TTGAAAGCCT AAAGCGCAGT 
CTCATGAGCA CCAATTTACA TCCATCCAAG CAGGTTAAAA CCTCCAGCCA GTATACGATA 
TTTTCCAAGC CACAAATTGC GACCACAACA CCGCAGACAC AGGTGTCGCC TGGTATGCCT 
TTGATGTTTC CCGGCGGAAG TCCCAATTTT GTGCAGGATA TCAAGATGGA GAATGGATTC 
ATGGATGTGG ATAGCCAGAG TAGCCAGTGT CCATCAGTGG AACGCAATTT CGCTTCGCCT 
CGATCCAACT GCAGTACAGT GGATAGTATT CCGCCGATGC AAATGGGGCA AAATCAAACT 
CATCTTTATT TGCCAGATGC CACAAACTTC ACATTTAATG GGAATTTCGC ATCGCCTTCG 
TCCAATTGCA GTACAGTGGA TAGCATTCCG CCGTTTCAAA TCGGACAGAG GAATAACCAT 
ATGTATCTGC CGGAGAATTC AAACTTTCCG GTAAACGGCT GTAGTCCTAC CCATTTCAGT 
GGTGGCTCTA TGACACCAAT CAATAATAAC AACAATGTAC TAATTAATAA CAATAACAAT 
GACTTTTTAA GTCAGAAAAT GAGTGCCATA AGCATACCGC CACAAGGAAA TTTTGGCATT 
AAACAGGTAT ATCAACAGAC TCAGCAGTTC CTGCCGCAAT TGCAGCCAGA GTCCATTCCA 
TATCTGGCCC AATCCCATCC AGAACAGTCG CAGTACCAAC AGCAACAGCA GCCACAGGAG 
CAGCAACCTC CAGCAGATGA ACCCACCCAA TCGTTTAGTG ATCTAATTTC CAGCAGCATA 
GGCATGGCGC CCATTGACAC CAGCGAACTT ATCCAGGACA TTGAGGCAGA ACTCAATAGC 
TTGGGAATTC AGCCATTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dif ( NM_078865.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523589.2 Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14819
Clone ID Related Accession (Same CDS sequence)	NM_165216.3 , NM_001169528.1 , NM_078865.4
Accession Version	NM_078865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dorsal-related immunity factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078865.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGAGG AGGCTTTCGG CGATATACAG GAAATTATCA ATGCCAGCAT GGAGTTGAAT 
GGTGGCGCCA CCGGCGGAGG CAGTGTGGCA GGAGCTGTTG GCGGAGGCGG TGCTGCACAT 
CACATATTAT CGCAGTCGAC TTCCCTGCCG GTAATGCCGT CGCACATTCC GCTCCACCTG 
CAGAATCAGA ATATGAATCA GAATCTGCCC GAGCCAAGTG CAAGAAGTGG TCCCCACCTG 
CGTATCGTGG AGGAGCCGAC AAGCAATATA ATCCGCTTTC GCTACAAATG CGAGGGTCGC 
ACCGCCGGTT CGATTCCGGG CATGAACTCC AGCTCGGAAA CGGGCAAGAC CTTTCCCACC 
ATCGAAGTGT GCAACTACGA TGGACCCGTC ATCATCGTGG TCTCCTGTGT GACGAGCGAC 
GAGCCCTTCC GCCAGCATCC CCACTGGCTG GTCAGCAAGG AGGAGGCGGA TGCCTGCAAG 
TCGGGCATCT ACCAAAAGAA ATTGCCGCCA GAGGAGCGGC GGCTGGTGCT CCAAAAAGTG 
GGCATACAGT GCGCCAAGAA GCTGGAGATG CGCGACTCGC TGGTGGAGAG GGAAAGGAGA 
AACATCGATC CCTTCAATGC CAAATTCGAT CACAAGGACC AGATCGACAA GATCAATCGG 
TATGAGTTGC GCCTCTGCTA CCAGGCCTTC ATCACAGTGG GCAACTCAAA AGTGCCCCTG 
GATCCCATCG TATCCTCACC GATTTACGGC AAGAGCAGCG AACTGACCAT CACCCGGCTG 
TGCAGTTGCG CGGCCACTGC GAACGGCGGC GACGAGATCA TCATGCTGTG CGAGAAGATT 
GCCAAGGACG ACATCGAGGT GCGATTCTAT GAGACGGACA AGGATGGACG GGAGACGTGG 
TTCGCCAACG CAGAGTTCCA ACCCACGGAC GTGTTCAAAC AGATGGCCAT AGCTTTTAAG 
ACGCCGCGCT ACAGGAACAC CGAGATCACA CAAAGTGTTA ATGTGGAGCT GAAACTAGTG 
AGACCCTCGG ATGGAGCGAC GAGTGCCCCA CTGCCGTTCG AGTACTACCC GAATCCAGAA 
CTCCTAACCA AACACAATCG CCGTGTTGCC CAAAAAACAG TTGAAAGCCT AAAGCGCAGT 
CTCATGAGCA CCAATTTACA TCCATCCAAG CAGGTTAAAA CCTCCAGCCA GTATACGATA 
TTTTCCAAGC CACAAATTGC GACCACAACA CCGCAGACAC AGGTGTCGCC TGGTATGCCT 
TTGATGTTTC CCGGCGGAAG TCCCAATTTT GTGCAGGATA TCAAGATGGA GAATGGATTC 
ATGGATGTGG ATAGCCAGAG TAGCCAGTGT CCATCAGTGG AACGCAATTT CGCTTCGCCT 
CGATCCAACT GCAGTACAGT GGATAGTATT CCGCCGATGC AAATGGGGCA AAATCAAACT 
CATCTTTATT TGCCAGATGC CACAAACTTC ACATTTAATG GGAATTTCGC ATCGCCTTCG 
TCCAATTGCA GTACAGTGGA TAGCATTCCG CCGTTTCAAA TCGGACAGAG GAATAACCAT 
ATGTATCTGC CGGAGAATTC AAACTTTCCG GTAAACGGCT GTAGTCCTAC CCATTTCAGT 
GGTGGCTCTA TGACACCAAT CAATAATAAC AACAATGTAC TAATTAATAA CAATAACAAT 
GACTTTTTAA GTCAGAAAAT GAGTGCCATA AGCATACCGC CACAAGGAAA TTTTGGCATT 
AAACAGGTAT ATCAACAGAC TCAGCAGTTC CTGCCGCAAT TGCAGCCAGA GTCCATTCCA 
TATCTGGCCC AATCCCATCC AGAACAGTCG CAGTACCAAC AGCAACAGCA GCCACAGGAG 
CAGCAACCTC CAGCAGATGA ACCCACCCAA TCGTTTAGTG ATCTAATTTC CAGCAGCATA 
GGCATGGCGC CCATTGACAC CAGCGAACTT ATCCAGGACA TTGAGGCAGA ACTCAATAGC 
TTGGGAATTC AGCCATTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523589.2
CDS	565..2568
Translation	MFEEAFGDIQEIINASMELNGGATGGGSVAGAVGGGGAAHHILSQSTSLPVMPSHIPLHLQNQNMNQNLPEPSARSGPHLRIVEEPTSNIIRFRYKCEGRTAGSIPGMNSSSETGKTFPTIEVCNYDGPVIIVVSCVTSDEPFRQHPHWLVSKEEADACKSGIYQKKLPPEERRLVLQKVGIQCAKKLEMRDSLVERERRNIDPFNAKFDHKDQIDKINRYELRLCYQAFITVGNSKVPLDPIVSSPIYGKSSELTITRLCSCAATANGGDEIIMLCEKIAKDDIEVRFYETDKDGRETWFANAEFQPTDVFKQMAIAFKTPRYRNTEITQSVNVELKLVRPSDGATSAPLPFEYYPNPELLTKHNRRVAQKTVESLKRSLMSTNLHPSKQVKTSSQYTIFSKPQIATTTPQTQVSPGMPLMFPGGSPNFVQDIKMENGFMDVDSQSSQCPSVERNFASPRSNCSTVDSIPPMQMGQNQTHLYLPDATNFTFNGNFASPSSNCSTVDSIPPFQIGQRNNHMYLPENSNFPVNGCSPTHFSGGSMTPINNNNNVLINNNNNDFLSQKMSAISIPPQGNFGIKQVYQQTQQFLPQLQPESIPYLAQSHPEQSQYQQQQQPQEQQPPADEPTQSFSDLISSSIGMAPIDTSELIQDIEAELNSLGIQPFK

Target ORF information:

RefSeq Version	NM_078865.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078865.4

ORF Insert Sequence:

ATGTTTGAGG AGGCTTTCGG CGATATACAG GAAATTATCA ATGCCAGCAT GGAGTTGAAT 
GGTGGCGCCA CCGGCGGAGG CAGTGTGGCA GGAGCTGTTG GCGGAGGCGG TGCTGCACAT 
CACATATTAT CGCAGTCGAC TTCCCTGCCG GTAATGCCGT CGCACATTCC GCTCCACCTG 
CAGAATCAGA ATATGAATCA GAATCTGCCC GAGCCAAGTG CAAGAAGTGG TCCCCACCTG 
CGTATCGTGG AGGAGCCGAC AAGCAATATA ATCCGCTTTC GCTACAAATG CGAGGGTCGC 
ACCGCCGGTT CGATTCCGGG CATGAACTCC AGCTCGGAAA CGGGCAAGAC CTTTCCCACC 
ATCGAAGTGT GCAACTACGA TGGACCCGTC ATCATCGTGG TCTCCTGTGT GACGAGCGAC 
GAGCCCTTCC GCCAGCATCC CCACTGGCTG GTCAGCAAGG AGGAGGCGGA TGCCTGCAAG 
TCGGGCATCT ACCAAAAGAA ATTGCCGCCA GAGGAGCGGC GGCTGGTGCT CCAAAAAGTG 
GGCATACAGT GCGCCAAGAA GCTGGAGATG CGCGACTCGC TGGTGGAGAG GGAAAGGAGA 
AACATCGATC CCTTCAATGC CAAATTCGAT CACAAGGACC AGATCGACAA GATCAATCGG 
TATGAGTTGC GCCTCTGCTA CCAGGCCTTC ATCACAGTGG GCAACTCAAA AGTGCCCCTG 
GATCCCATCG TATCCTCACC GATTTACGGC AAGAGCAGCG AACTGACCAT CACCCGGCTG 
TGCAGTTGCG CGGCCACTGC GAACGGCGGC GACGAGATCA TCATGCTGTG CGAGAAGATT 
GCCAAGGACG ACATCGAGGT GCGATTCTAT GAGACGGACA AGGATGGACG GGAGACGTGG 
TTCGCCAACG CAGAGTTCCA ACCCACGGAC GTGTTCAAAC AGATGGCCAT AGCTTTTAAG 
ACGCCGCGCT ACAGGAACAC CGAGATCACA CAAAGTGTTA ATGTGGAGCT GAAACTAGTG 
AGACCCTCGG ATGGAGCGAC GAGTGCCCCA CTGCCGTTCG AGTACTACCC GAATCCAGAA 
CTCCTAACCA AACACAATCG CCGTGTTGCC CAAAAAACAG TTGAAAGCCT AAAGCGCAGT 
CTCATGAGCA CCAATTTACA TCCATCCAAG CAGGTTAAAA CCTCCAGCCA GTATACGATA 
TTTTCCAAGC CACAAATTGC GACCACAACA CCGCAGACAC AGGTGTCGCC TGGTATGCCT 
TTGATGTTTC CCGGCGGAAG TCCCAATTTT GTGCAGGATA TCAAGATGGA GAATGGATTC 
ATGGATGTGG ATAGCCAGAG TAGCCAGTGT CCATCAGTGG AACGCAATTT CGCTTCGCCT 
CGATCCAACT GCAGTACAGT GGATAGTATT CCGCCGATGC AAATGGGGCA AAATCAAACT 
CATCTTTATT TGCCAGATGC CACAAACTTC ACATTTAATG GGAATTTCGC ATCGCCTTCG 
TCCAATTGCA GTACAGTGGA TAGCATTCCG CCGTTTCAAA TCGGACAGAG GAATAACCAT 
ATGTATCTGC CGGAGAATTC AAACTTTCCG GTAAACGGCT GTAGTCCTAC CCATTTCAGT 
GGTGGCTCTA TGACACCAAT CAATAATAAC AACAATGTAC TAATTAATAA CAATAACAAT 
GACTTTTTAA GTCAGAAAAT GAGTGCCATA AGCATACCGC CACAAGGAAA TTTTGGCATT 
AAACAGGTAT ATCAACAGAC TCAGCAGTTC CTGCCGCAAT TGCAGCCAGA GTCCATTCCA 
TATCTGGCCC AATCCCATCC AGAACAGTCG CAGTACCAAC AGCAACAGCA GCCACAGGAG 
CAGCAACCTC CAGCAGATGA ACCCACCCAA TCGTTTAGTG ATCTAATTTC CAGCAGCATA 
GGCATGGCGC CCATTGACAC CAGCGAACTT ATCCAGGACA TTGAGGCAGA ACTCAATAGC 
TTGGGAATTC AGCCATTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dif ( NM_001169527.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162998.1 Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant C, mRNA.

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CloneID	OFa16875
Clone ID Related Accession (Same CDS sequence)	NM_001169527.2
Accession Version	NM_001169527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dorsal-related immunity factor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169527.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGAGG AGGCTTTCGG CGATATACAG GAAATTATCA ATGCCAGCAT GGAGTTGAAT 
GGTGGCGCCA CCGGCGGAGG CAGTGTGGCA GGAGCTGTTG GCGGAGGCGG TGCTGCACAT 
CACATATTAT CGCAGTCGAC TTCCCTGCCG GTAATGCCGT CGCACATTCC GCTCCACCTG 
CAGAATCAGA ATATGAATCA GAATCTGCCC GAGCCAAGTG CAAGAAGTGG TCCCCACCTG 
CGTATCGTGG AGGAGCCGAC AAGCAATATA ATCCGCTTTC GCTACAAATG CGAGGGTCGC 
ACCGCCGGTT CGATTCCGGG CATGAACTCC AGCTCGGAAA CGGGCAAGAC CTTTCCCACC 
ATCGAAGTGT GCAACTACGA TGGACCCGTC ATCATCGTGG TCTCCTGTGT GACGAGCGAC 
GAGCCCTTCC GCCAGCATCC CCACTGGCTG GTCAGCAAGG AGGAGGCGGA TGCCTGCAAG 
TCGGGCATCT ACCAAAAGAA ATTGCCGCCA GAGGAGCGGC GGCTGGTGCT CCAAAAAGTG 
GGCATACAGT GCGCCAAGAA GCTGGAGATG CGCGACTCGC TGGTGGAGAG GGAAAGGAGA 
AACATCGATC CCTTCAATGC CAAATTCGAT CACAAGGACC AGATCGACAA GATCAATCGG 
TATGAGTTGC GCCTCTGCTA CCAGGCCTTC ATCACAGTGG GCAACTCAAA AGTGCCCCTG 
GATCCCATCG TATCCTCACC GATTTACGGC AAGAGCAGCG AACTGACCAT CACCCGGCTG 
TGCAGTTGCG CGGCCACTGC GAACGGCGGC GACGAGATCA TCATGCTGTG CGAGAAGATT 
GCCAAGGACG ACATCGAGGT GCGATTCTAT GAGACGGACA AGGATGGACG GGAGACGTGG 
TTCGCCAACG CAGAGTTCCA ACCCACGGAC GTGTTCAAAC AGATGGCCAT AGCTTTTAAG 
ACGCCGCGCT ACAGGAACAC CGAGATCACA CAAAGTGTTA ATGTGGAGCT GAAACTAGTG 
AGACCCTCGG ATGGAGCGAC GAGTGCCCCA CTGCCGTTCG AGTACTACCC GAATCCAGGT 
ACAGTAACCT TTGCCCGGCT ACAGCGAAAG TTGAAACGCC GCCAGGAACT GGACGTGTTC 
CAGCAGATCC TTTCCCTAGA CAGCGAAACG ACGGCCACGA AATACTCGTG CCCACCGATG 
GATCTGGAGG AGGATAATAA CACGGTTTCA TCCGATGACC AACAGAGTTC GGGAACTCTC 
AACGAATTCG AGATAGCCGT CAAGAAAATG CAAATGCGTC AGAGGGGTGA ATCGCACGAA 
ATGGATGGTG GCACTGTCAC CGAGTACACG GATAACGATA ACGAACCAGA TATGGAAATA 
GAGCTGGAGG TTCCGCAATT GTTGCCCCAA CTGGCCGATG ACTGCACCCA GACGTCCACG 
CCGATGGAGG AGATTCTGCA GCATCCGCAG CTGCAATCGC GACCCCTGTC CAATGTGGAA 
ACAATCACCG AGTGGATGAA GAGCAGCGAG TTCGAGAGAA CGGATAGCCT GACGGTGGAG 
AACGGGGACA CGCAATCCCT ATCGAATAGC ACGGCCCAAA CAGCATTTAC CAATGTCAGC 
AGCTTGACAG CCAATACGAC CATAACGAAC CAGTTGGACG AGGAAGCCCA CGGTCTTTTG 
GCCAATGGTT CTCGCAGGGA TACCCTCGAG AATCCGGCCG TGAAAGAGCT ACCGGAAAAT 
GAAAAAGAAC CGGAAATGGT TTCAGCCAGT GCCCAGGCCT CTATGGATTT AGATGAAAAC 
TTCGATGAGA CAGCCACATA TACGAGCCTA CAAATAGCCT TTAATAATCC CATAGACATA 
GGATTGCCCA AAGAAAGCAA ACAAGCAGCC CCCATTTATC CACCCACCAA TCCGTTTGGT 
CAGCTAGATG ATGACGAACT GGTGGTGCTG ACAAATCCAC CAGCTCCCAG GATCAAAGTG 
AACGCTGCTC AGACACCAGC CACGCCCCCA GCCTCACCGC CCCTGCCATT GCCACCTCGA 
ACTCCTTCCC CAGTTTCAGA TCAGAGATTA CCTCCCTTAC CACCGAAACC GCAGAGAAAC 
TCCCAGGATC TGAGTGCTGT CAGTGATTCA ATTTCTGTGA GCCGCTCGCG AAACGGCAGC 
CTAAGCTCCA ATAGAACTAC ACCTTCGCCT ATAATTATAA TGCGAACCCC TGATCAAAGT 
CCCACCAAAA GGCAACCGAC GCCCAGTGCT TCGCCAAAGA AGCGTCAGGG ATTCTTCTCG 
AGATTCTTTT CTCGCCGAAG GAGCAAGACG GATGACGAAG AATCTCTGAC ACCTGGTGGC 
AGCCCCAATA AGCAACTACC AGGGGGATCG AAAGCCACAA CACCCACCGG CAGTAGGGAA 
CCAAGCGTTG CGCACTTTTC CCTGGGAGAT CCCAATCGCA GCTCCACAAG ATCGATGCAG 
CCACTGGGAA AAAGTGAGGG TCGAAATGGC AAGCCAGTCG GTCGTAGTTC GAGCAGTGTA 
TCGGGTAAAA GACCTGCCCA CCTGGATGCG GATGTTATTC ACATACCATT GAAGGGAGGC 
GATAGCGAGA ACAGTTTGCT ACGACCGGAG AGCTACTCGA ATGCCAGCAC CCTTAGTTAC 
GGTCGACCGC TGGATAGGAA GACCCTGAGC ACTCTGCAAC TGGCGGATAT TCCCATAAGT 
GACGGCAATA TGGAATTGAT TGCCATCGCC GACAGGCAGA GCATCAAGAA TCTGTGCGAG 
GGAGCCTATG GTGTTGTGCT GGATCCCAGT GTGGATCTCT CCGAGGCGGA ACACTTTGCT 
TTGTACACCA GCAAAAGTCC GGAACCACCA TTTGGCGGGG AGTGCGGCGA AGGTGGAGCA 
GCAACTGGTG GAGTGGATAC GACCGATTTT TTGAGTGCGG ATGAAATAGC ACGGCGGCTG 
GCGGAGGCAA ATGGCTTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162998.1
CDS	565..3528
Translation	MFEEAFGDIQEIINASMELNGGATGGGSVAGAVGGGGAAHHILSQSTSLPVMPSHIPLHLQNQNMNQNLPEPSARSGPHLRIVEEPTSNIIRFRYKCEGRTAGSIPGMNSSSETGKTFPTIEVCNYDGPVIIVVSCVTSDEPFRQHPHWLVSKEEADACKSGIYQKKLPPEERRLVLQKVGIQCAKKLEMRDSLVERERRNIDPFNAKFDHKDQIDKINRYELRLCYQAFITVGNSKVPLDPIVSSPIYGKSSELTITRLCSCAATANGGDEIIMLCEKIAKDDIEVRFYETDKDGRETWFANAEFQPTDVFKQMAIAFKTPRYRNTEITQSVNVELKLVRPSDGATSAPLPFEYYPNPGTVTFARLQRKLKRRQELDVFQQILSLDSETTATKYSCPPMDLEEDNNTVSSDDQQSSGTLNEFEIAVKKMQMRQRGESHEMDGGTVTEYTDNDNEPDMEIELEVPQLLPQLADDCTQTSTPMEEILQHPQLQSRPLSNVETITEWMKSSEFERTDSLTVENGDTQSLSNSTAQTAFTNVSSLTANTTITNQLDEEAHGLLANGSRRDTLENPAVKELPENEKEPEMVSASAQASMDLDENFDETATYTSLQIAFNNPIDIGLPKESKQAAPIYPPTNPFGQLDDDELVVLTNPPAPRIKVNAAQTPATPPASPPLPLPPRTPSPVSDQRLPPLPPKPQRNSQDLSAVSDSISVSRSRNGSLSSNRTTPSPIIIMRTPDQSPTKRQPTPSASPKKRQGFFSRFFSRRRSKTDDEESLTPGGSPNKQLPGGSKATTPTGSREPSVAHFSLGDPNRSSTRSMQPLGKSEGRNGKPVGRSSSSVSGKRPAHLDADVIHIPLKGGDSENSLLRPESYSNASTLSYGRPLDRKTLSTLQLADIPISDGNMELIAIADRQSIKNLCEGAYGVVLDPSVDLSEAEHFALYTSKSPEPPFGGECGEGGAATGGVDTTDFLSADEIARRLAEANGLE

Target ORF information:

RefSeq Version	NM_001169527.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dorsal-related immunity factor (Dif), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169527.2

ORF Insert Sequence:

ATGTTTGAGG AGGCTTTCGG CGATATACAG GAAATTATCA ATGCCAGCAT GGAGTTGAAT 
GGTGGCGCCA CCGGCGGAGG CAGTGTGGCA GGAGCTGTTG GCGGAGGCGG TGCTGCACAT 
CACATATTAT CGCAGTCGAC TTCCCTGCCG GTAATGCCGT CGCACATTCC GCTCCACCTG 
CAGAATCAGA ATATGAATCA GAATCTGCCC GAGCCAAGTG CAAGAAGTGG TCCCCACCTG 
CGTATCGTGG AGGAGCCGAC AAGCAATATA ATCCGCTTTC GCTACAAATG CGAGGGTCGC 
ACCGCCGGTT CGATTCCGGG CATGAACTCC AGCTCGGAAA CGGGCAAGAC CTTTCCCACC 
ATCGAAGTGT GCAACTACGA TGGACCCGTC ATCATCGTGG TCTCCTGTGT GACGAGCGAC 
GAGCCCTTCC GCCAGCATCC CCACTGGCTG GTCAGCAAGG AGGAGGCGGA TGCCTGCAAG 
TCGGGCATCT ACCAAAAGAA ATTGCCGCCA GAGGAGCGGC GGCTGGTGCT CCAAAAAGTG 
GGCATACAGT GCGCCAAGAA GCTGGAGATG CGCGACTCGC TGGTGGAGAG GGAAAGGAGA 
AACATCGATC CCTTCAATGC CAAATTCGAT CACAAGGACC AGATCGACAA GATCAATCGG 
TATGAGTTGC GCCTCTGCTA CCAGGCCTTC ATCACAGTGG GCAACTCAAA AGTGCCCCTG 
GATCCCATCG TATCCTCACC GATTTACGGC AAGAGCAGCG AACTGACCAT CACCCGGCTG 
TGCAGTTGCG CGGCCACTGC GAACGGCGGC GACGAGATCA TCATGCTGTG CGAGAAGATT 
GCCAAGGACG ACATCGAGGT GCGATTCTAT GAGACGGACA AGGATGGACG GGAGACGTGG 
TTCGCCAACG CAGAGTTCCA ACCCACGGAC GTGTTCAAAC AGATGGCCAT AGCTTTTAAG 
ACGCCGCGCT ACAGGAACAC CGAGATCACA CAAAGTGTTA ATGTGGAGCT GAAACTAGTG 
AGACCCTCGG ATGGAGCGAC GAGTGCCCCA CTGCCGTTCG AGTACTACCC GAATCCAGGT 
ACAGTAACCT TTGCCCGGCT ACAGCGAAAG TTGAAACGCC GCCAGGAACT GGACGTGTTC 
CAGCAGATCC TTTCCCTAGA CAGCGAAACG ACGGCCACGA AATACTCGTG CCCACCGATG 
GATCTGGAGG AGGATAATAA CACGGTTTCA TCCGATGACC AACAGAGTTC GGGAACTCTC 
AACGAATTCG AGATAGCCGT CAAGAAAATG CAAATGCGTC AGAGGGGTGA ATCGCACGAA 
ATGGATGGTG GCACTGTCAC CGAGTACACG GATAACGATA ACGAACCAGA TATGGAAATA 
GAGCTGGAGG TTCCGCAATT GTTGCCCCAA CTGGCCGATG ACTGCACCCA GACGTCCACG 
CCGATGGAGG AGATTCTGCA GCATCCGCAG CTGCAATCGC GACCCCTGTC CAATGTGGAA 
ACAATCACCG AGTGGATGAA GAGCAGCGAG TTCGAGAGAA CGGATAGCCT GACGGTGGAG 
AACGGGGACA CGCAATCCCT ATCGAATAGC ACGGCCCAAA CAGCATTTAC CAATGTCAGC 
AGCTTGACAG CCAATACGAC CATAACGAAC CAGTTGGACG AGGAAGCCCA CGGTCTTTTG 
GCCAATGGTT CTCGCAGGGA TACCCTCGAG AATCCGGCCG TGAAAGAGCT ACCGGAAAAT 
GAAAAAGAAC CGGAAATGGT TTCAGCCAGT GCCCAGGCCT CTATGGATTT AGATGAAAAC 
TTCGATGAGA CAGCCACATA TACGAGCCTA CAAATAGCCT TTAATAATCC CATAGACATA 
GGATTGCCCA AAGAAAGCAA ACAAGCAGCC CCCATTTATC CACCCACCAA TCCGTTTGGT 
CAGCTAGATG ATGACGAACT GGTGGTGCTG ACAAATCCAC CAGCTCCCAG GATCAAAGTG 
AACGCTGCTC AGACACCAGC CACGCCCCCA GCCTCACCGC CCCTGCCATT GCCACCTCGA 
ACTCCTTCCC CAGTTTCAGA TCAGAGATTA CCTCCCTTAC CACCGAAACC GCAGAGAAAC 
TCCCAGGATC TGAGTGCTGT CAGTGATTCA ATTTCTGTGA GCCGCTCGCG AAACGGCAGC 
CTAAGCTCCA ATAGAACTAC ACCTTCGCCT ATAATTATAA TGCGAACCCC TGATCAAAGT 
CCCACCAAAA GGCAACCGAC GCCCAGTGCT TCGCCAAAGA AGCGTCAGGG ATTCTTCTCG 
AGATTCTTTT CTCGCCGAAG GAGCAAGACG GATGACGAAG AATCTCTGAC ACCTGGTGGC 
AGCCCCAATA AGCAACTACC AGGGGGATCG AAAGCCACAA CACCCACCGG CAGTAGGGAA 
CCAAGCGTTG CGCACTTTTC CCTGGGAGAT CCCAATCGCA GCTCCACAAG ATCGATGCAG 
CCACTGGGAA AAAGTGAGGG TCGAAATGGC AAGCCAGTCG GTCGTAGTTC GAGCAGTGTA 
TCGGGTAAAA GACCTGCCCA CCTGGATGCG GATGTTATTC ACATACCATT GAAGGGAGGC 
GATAGCGAGA ACAGTTTGCT ACGACCGGAG AGCTACTCGA ATGCCAGCAC CCTTAGTTAC 
GGTCGACCGC TGGATAGGAA GACCCTGAGC ACTCTGCAAC TGGCGGATAT TCCCATAAGT 
GACGGCAATA TGGAATTGAT TGCCATCGCC GACAGGCAGA GCATCAAGAA TCTGTGCGAG 
GGAGCCTATG GTGTTGTGCT GGATCCCAGT GTGGATCTCT CCGAGGCGGA ACACTTTGCT 
TTGTACACCA GCAAAAGTCC GGAACCACCA TTTGGCGGGG AGTGCGGCGA AGGTGGAGCA 
GCAACTGGTG GAGTGGATAC GACCGATTTT TTGAGTGCGG ATGAAATAGC ACGGCGGCTG 
GCGGAGGCAA ATGGCTTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dif cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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