Tif-IA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tif-IA
Entrez Gene ID	35454
Full Name	CG3278 gene product from transcript CG3278-RA
Synonyms	CG16938,CG3278,CG5951,Dmel\CG3278,TIF-1A,TIF-IA,TIf-IA,Tif-1A,TifIA,dTIF-IA,tif1a
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 40F6-40F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_176070.3	NP_788084.1	Tif-IA

Pathways from BioSystems

Homologies

	RRN3	XP_001108222.1
Neurospora crassa	NCU09247	XP_964682.1
Homo sapiens (human)	RRN3	NP_060897.3
Mus musculus (house mouse)	Rrn3	NP_001034610.1
Rattus norvegicus (Norway rat)	Rrn3	NP_001129318.1
Danio rerio (zebrafish)	rrn3	XP_706962.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009024	XP_319773.4
Arabidopsis thaliana (thale cress)	AT2G39240	NP_181457.2
Oryza sativa (rice)	Os06g0688100	NP_001058413.2
Bos taurus (cattle)	RRN3	NP_001179361.2
Schizosaccharomyces pombe (fission yeast)	rrn3	NP_593674.1
Magnaporthe oryzae (rice blast fungus)	MGG_01654	XP_003714640.1
Arabidopsis thaliana (thale cress)	AT1G30590	NP_174347.1
Pan troglodytes (chimpanzee)	RRN3	XP_001143837.2
Canis lupus familiaris (dog)	RRN3	XP_536965.3
Gallus gallus (chicken)	RRN3	NP_001264085.1
Drosophila melanogaster (fruit fly)	Tif-IA	NP_788084.1
Caenorhabditis elegans (roundworm)	C36E8.1	NP_497803.1
Arabidopsis thaliana (thale cress)	AT2G34750	NP_850236.1
Xenopus tropicalis (tropical clawed frog)	rrn3	XP_004918010.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A role of Tif-1a in germline stem cells maintenance
Title: A Combination of CRISPR/Cas9 and Standardized RNAi as a Versatile Platform for the Characterization of Gene Function.

muscle ribosome synthesis functions as a nutrient-dependent checkpoint for overall body growth: in nutrient rich conditions, TOR is required to maintain levels of TIF-IA and ribosome synthesis to promote high levels of systemic insulin
Title: TIF-IA-dependent regulation of ribosome synthesis in drosophila muscle is required to maintain systemic insulin signaling and larval growth.

The following Tif-IA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tif-IA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15174	NM_176070.3 Latest version!	Drosophila melanogaster Tif-IA (Tif-IA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tif-IA ( NM_176070.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788084.1 Drosophila melanogaster Tif-IA (Tif-IA), mRNA.

Price & Availability↑

CloneID	OFa15174
Clone ID Related Accession (Same CDS sequence)	NM_176070.3
Accession Version	NM_176070.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Tif-IA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_176070.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTTCT ACACGAGTAA GACGGATGTG ACTTCCATTT TAAAGACGTT TTCTGGCGTA 
AAACGAAATC AGGCAAATGC CGTGGCCATC AACAAAGTTC GATTTTCCAC ACCCAAAGAG 
AAGGGGCTCG CAGAATCAGT GCGCGTTGCC CTAGAAGAAC GAAATTTCCA TCTGGTCAAG 
GAGTTCACAT ACTTCCTCCG GGAAGCAGAA CTCAGCGATG ATGAGGTTGT AATAATCATA 
AAAGATGCTA AACGAATTGT TCATAATTTG ACTCCAGAAT TTGTAACCGT TGTCGAGGCG 
TTACTGTCCC TTAACTGGAA GAAAAGAAGT TCAGAGATAA TCGAAGCTTA TACTGAATTT 
TGTATCGAAC TAATGGTGAC CCATAATAGA TATTTACCGA TTGGAGTCTC TAAACTAATT 
GTCCACTGGA TTCCTGGAGA TATGGATGAA TCCGATTGGG TCCACGGATG CCCTTCGGAG 
CACGTGCGAA ATGAGTTGAA ACCCATCCAT AAAGTACTGG ATCGCATCTT AACTGCCGTG 
CCAATGGCTT TCGATATTAT CATAGATGCC ATATCCGCCA AATTCCCCTA TTTCAAAAAG 
CCGTACCATG TGACGGCAGG GTATCTGTAT AACGTGCTTT GGTTATTAGA ATACAAGCCT 
ATTTTCGAAG AACTTGTTTT TCAACTAGTT TTACAAAAGC TTTTATTATT AGATGTTAAT 
GCTCCAAAAG ATGAAATTGA ATTAGAAATG AATGATGAGG AGGAAAAGGT GGAAGCGGAC 
ACCTTGTTTG AAATCGACGA TGTATGCGCC GAAAATGTAC CCAAATCTGA AAAATCTGTG 
AGTCATCCTG TTGGCAAAAC CTTGGATGTA TGCCTATTCT TGCTTTATAA GTTTTTTGAT 
GGCAAATGTC GCCTTAATGA AAATAGTAGC GAAGAACAGC GAAGGACTGC CAACCGATTG 
TTTAAGATGT TACTTTATTT GTTCGACGAG GTGTTATTGC CCAGCCACAA CACGCATCAT 
GTGCAATTCA TCCTATTTTA TGCAACTAGT CTGCGGTCGG TATATTCCGA AGCTTTTCTT 
GATTCCTTAT GGACGAAAGT CCAAAATCCG AATGTATCCG CTGTAATTCG ACATGCTGCG 
GTGGGATACA TGGCTAGCTT TTTATCTCGC GCACGATTTT TGCCGCTTAG CACTGTTACT 
TTCTACCTAA AAGAGTTGAC AAGGTGGGCT CATACATACA TAGATGACTC CGATGCATAT 
AAGCAGAACT GCTCTCTGAA AGCCAATTTG GTTTTCTATT CTGTTTGCCA AGCTGTGTTT 
TATTTAATTG CATTTCGCGC AAAAGATTTG ACGGCCAGTT CAAAAGATTT GCTCTTCCTC 
CAATCCCTCC AACTATCGCG CCTTGCTATG TGTCATTTAA ATCCCCTGCG GTATTGTTTA 
GCCCCAGTGG CCACAGCATT TGCTGGTGTT ACAAGAACTT ACCAGCTGGC CTATTGTCAT 
ACTGTTTTGG AGCGCAACGC TCGCCGTAAA CTGGCTACAG TTTACGGTCA TGAAAAATGC 
ATGCCCGATG AGACGCTGGA ATCGTTTTTT CCCTTCGATC CTTATGTACT TAAGTTATCA 
AAAAAATATA TTGAGACTAA TTATATGGTT TACCAGAGCA ATGACACGGA TGAGTATGTC 
TATGGGTCTA ACAAATACGG GCATTCTAGG AAACGTGGTG ACTCTGAAAT GCTAGAGGAA 
GACGAATTCC TAATTGTCGA CAAACGTCCA AAGAATTTTG ATCTGTCTAA AAGTCAGGAA 
TTTGACAAAC AATTTCATTT CGGATCTTCG CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788084.1
CDS	224..2059
Translation	MSFYTSKTDVTSILKTFSGVKRNQANAVAINKVRFSTPKEKGLAESVRVALEERNFHLVKEFTYFLREAELSDDEVVIIIKDAKRIVHNLTPEFVTVVEALLSLNWKKRSSEIIEAYTEFCIELMVTHNRYLPIGVSKLIVHWIPGDMDESDWVHGCPSEHVRNELKPIHKVLDRILTAVPMAFDIIIDAISAKFPYFKKPYHVTAGYLYNVLWLLEYKPIFEELVFQLVLQKLLLLDVNAPKDEIELEMNDEEEKVEADTLFEIDDVCAENVPKSEKSVSHPVGKTLDVCLFLLYKFFDGKCRLNENSSEEQRRTANRLFKMLLYLFDEVLLPSHNTHHVQFILFYATSLRSVYSEAFLDSLWTKVQNPNVSAVIRHAAVGYMASFLSRARFLPLSTVTFYLKELTRWAHTYIDDSDAYKQNCSLKANLVFYSVCQAVFYLIAFRAKDLTASSKDLLFLQSLQLSRLAMCHLNPLRYCLAPVATAFAGVTRTYQLAYCHTVLERNARRKLATVYGHEKCMPDETLESFFPFDPYVLKLSKKYIETNYMVYQSNDTDEYVYGSNKYGHSRKRGDSEMLEEDEFLIVDKRPKNFDLSKSQEFDKQFHFGSSP

Target ORF information:

RefSeq Version	NM_176070.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Tif-IA (Tif-IA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176070.3

ORF Insert Sequence:

ATGTCCTTCT ACACGAGTAA GACGGATGTG ACTTCCATTT TAAAGACGTT TTCTGGCGTA 
AAACGAAATC AGGCAAATGC CGTGGCCATC AACAAAGTTC GATTTTCCAC ACCCAAAGAG 
AAGGGGCTCG CAGAATCAGT GCGCGTTGCC CTAGAAGAAC GAAATTTCCA TCTGGTCAAG 
GAGTTCACAT ACTTCCTCCG GGAAGCAGAA CTCAGCGATG ATGAGGTTGT AATAATCATA 
AAAGATGCTA AACGAATTGT TCATAATTTG ACTCCAGAAT TTGTAACCGT TGTCGAGGCG 
TTACTGTCCC TTAACTGGAA GAAAAGAAGT TCAGAGATAA TCGAAGCTTA TACTGAATTT 
TGTATCGAAC TAATGGTGAC CCATAATAGA TATTTACCGA TTGGAGTCTC TAAACTAATT 
GTCCACTGGA TTCCTGGAGA TATGGATGAA TCCGATTGGG TCCACGGATG CCCTTCGGAG 
CACGTGCGAA ATGAGTTGAA ACCCATCCAT AAAGTACTGG ATCGCATCTT AACTGCCGTG 
CCAATGGCTT TCGATATTAT CATAGATGCC ATATCCGCCA AATTCCCCTA TTTCAAAAAG 
CCGTACCATG TGACGGCAGG GTATCTGTAT AACGTGCTTT GGTTATTAGA ATACAAGCCT 
ATTTTCGAAG AACTTGTTTT TCAACTAGTT TTACAAAAGC TTTTATTATT AGATGTTAAT 
GCTCCAAAAG ATGAAATTGA ATTAGAAATG AATGATGAGG AGGAAAAGGT GGAAGCGGAC 
ACCTTGTTTG AAATCGACGA TGTATGCGCC GAAAATGTAC CCAAATCTGA AAAATCTGTG 
AGTCATCCTG TTGGCAAAAC CTTGGATGTA TGCCTATTCT TGCTTTATAA GTTTTTTGAT 
GGCAAATGTC GCCTTAATGA AAATAGTAGC GAAGAACAGC GAAGGACTGC CAACCGATTG 
TTTAAGATGT TACTTTATTT GTTCGACGAG GTGTTATTGC CCAGCCACAA CACGCATCAT 
GTGCAATTCA TCCTATTTTA TGCAACTAGT CTGCGGTCGG TATATTCCGA AGCTTTTCTT 
GATTCCTTAT GGACGAAAGT CCAAAATCCG AATGTATCCG CTGTAATTCG ACATGCTGCG 
GTGGGATACA TGGCTAGCTT TTTATCTCGC GCACGATTTT TGCCGCTTAG CACTGTTACT 
TTCTACCTAA AAGAGTTGAC AAGGTGGGCT CATACATACA TAGATGACTC CGATGCATAT 
AAGCAGAACT GCTCTCTGAA AGCCAATTTG GTTTTCTATT CTGTTTGCCA AGCTGTGTTT 
TATTTAATTG CATTTCGCGC AAAAGATTTG ACGGCCAGTT CAAAAGATTT GCTCTTCCTC 
CAATCCCTCC AACTATCGCG CCTTGCTATG TGTCATTTAA ATCCCCTGCG GTATTGTTTA 
GCCCCAGTGG CCACAGCATT TGCTGGTGTT ACAAGAACTT ACCAGCTGGC CTATTGTCAT 
ACTGTTTTGG AGCGCAACGC TCGCCGTAAA CTGGCTACAG TTTACGGTCA TGAAAAATGC 
ATGCCCGATG AGACGCTGGA ATCGTTTTTT CCCTTCGATC CTTATGTACT TAAGTTATCA 
AAAAAATATA TTGAGACTAA TTATATGGTT TACCAGAGCA ATGACACGGA TGAGTATGTC 
TATGGGTCTA ACAAATACGG GCATTCTAGG AAACGTGGTG ACTCTGAAAT GCTAGAGGAA 
GACGAATTCC TAATTGTCGA CAAACGTCCA AAGAATTTTG ATCTGTCTAA AAGTCAGGAA 
TTTGACAAAC AATTTCATTT CGGATCTTCG CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tif-IA cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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