BEAF-32 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	BEAF-32
Entrez Gene ID	36645
Full Name	Boundary element-associated factor of 32kD
Synonyms	BEAF,BEAF-32A,BEAF-32B,BEAF32,BEAF32A,BEAF32B,BEAF32b,Beaf-32,Beaf32,CG10159,Dmel\CG10159,beaf,bs04c01.y1
General protein information	Preferred Names Boundary element-associated factor of 32kD Names CG10159 gene product from transcript CG10159-RA BEAF-32-PA BEAF-32-PB BEAF-32-PC CG10159-PA CG10159-PB CG10159-PC boundary element associated factor boundary element associated factor 32 boundary element associated factor of 32 boundary element-associated factor boundary element-associated factor of 32kD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51C2-51C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_166069.3	NP_725418.1	boundary element-associated factor of 32kD, isoform B
NM_079023.5	NP_523747.1	boundary element-associated factor of 32kD, isoform A
NM_001259399.2	NP_001246328.1	boundary element-associated factor of 32kD, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identify key roles for the Zelda and Zerknullt transcription factors in establishing the resulting expression domain, and find widespread binding of insulator proteins to the Mad and Brinker-bound genomic regions. Analysis of embryos lacking the BEAF-32 insulator protein shows reduced transcription of a peak BMP target gene and a reduction in the number of amnioserosa cells, the fate specified by peak BMP signaling.
Title: Regulation of the BMP Signaling-Responsive Transcriptional Network in the Drosophila Embryo.

Loss of BEAF-32 does not noticeably impair the functions of the Hippo pathway in eye growth regulation.
Title: The insulator protein BEAF-32 is required for Hippo pathway activity in the terminal differentiation of neuronal subtypes.

The BESS domain alone is sufficient to mediate BEAF-BEAF interactions, although the presence of the putative leucine zipper on at least one protein strengthens the interactions. BEAF-32B is sufficient to rescue a null BEAF mutation. Using mutant BEAF-32B rescue transgenes, we show that the middle region and the BESS domain are essential.
Title: Characterization of the Drosophila BEAF-32A and BEAF-32B Insulator Proteins.

Miss-regulation of these genes explains the unregulated cell growth and neoplastic phenotypes observed in imaginal tissues of BEAF-32 mutants.
Title: The BEAF insulator regulates genes involved in cell polarity and neoplastic growth.

BEAF is a positive regulator of the chromatin domain boundary that separates myoglianin and eyeless genes.
Title: A BEAF dependent chromatin domain boundary separates myoglianin and eyeless genes of Drosophila melanogaster.

The following BEAF-32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BEAF-32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10020	NM_079023.5 Latest version!	Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant A (BEAF-32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa10021	NM_166069.3 Latest version!	Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant B (BEAF-32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa10021	NM_001259399.2 Latest version!	Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant C (BEAF-32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BEAF-32 ( NM_079023.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523747.1 Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant A (BEAF-32), mRNA.

Price & Availability↑

CloneID	OFa10020
Clone ID Related Accession (Same CDS sequence)	NM_079023.5
Accession Version	NM_079023.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	boundary element-associated factor of 32kD, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079023.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGTTG AAAAGAAATC AGAGCTTCGT AGCCTACTAA AATCCGGGGA CAAGCGTTGC 
AAACTCGTTG AGCCCAGGAA CACCAAAAGC TGCGTCTGGA GGTTTTTCAA TCTCGTCCAG 
TGCGACGACC ACATAGAGCC GTACGCCTGC TGCAAAACCT GTGGCGATCT GTTATCCTAC 
AGCGGCAAAA CTGGAACCGG ATCCCTGCTA CGGCACCGAT GTCTCCACTC CAGCAGCAGT 
AACGACAAAA CCGTGCGGAT TACCAAGGCC AAGACGCTGA GGGAGCCGCT GCGTGTGGCC 
AAGCCGAAGA TCGAGTCGAA CGTCGCCAAT TATCTGGGCG AGGCGGGCGC CCTGCCGCAA 
AAGTGGGAGG AGTACGAGCA GAACGATGAG ATTAGCGAGG ACATTAAGGA CATCATATAC 
AGCGAGGATC CACTGTGCTA TAGTCCTATA CACGTGATGG ATGATGAGGG CCTGGATCAG 
CCTGAGAAGC AAGTGACCGT GCTAACCCAT TCGACTTCAC CAGCCGGTGG AAGTAGCAGG 
CCCATCGGTG TTGGTTCGGG CGTACAGGCT ACTGTGGTGG CCTCCACGTC CTCCAGCAGC 
AGCTCGGCCA AGCAGCTGAA GAACAACCTG GAGACGTCCA TCGAACGGCT GACCGCTGTC 
AGCGAACAGC TCAGCTACAT CATACAACAG AATCACGAGG AGCTCACCAA GGACGACGAC 
TACTATTTTG CACTTAGCTT GGTGCCCGCA ATGCGCCACC TTTCGCTCAG TCGGAAGATG 
TACGTGCGGT CCAAGATCCA GGATATACTG TTCAAGGAAA GTGAGGACTC AACGCTTGCC 
AAGGATGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523747.1
CDS	118..969
Translation	MHVEKKSELRSLLKSGDKRCKLVEPRNTKSCVWRFFNLVQCDDHIEPYACCKTCGDLLSYSGKTGTGSLLRHRCLHSSSSNDKTVRITKAKTLREPLRVAKPKIESNVANYLGEAGALPQKWEEYEQNDEISEDIKDIIYSEDPLCYSPIHVMDDEGLDQPEKQVTVLTHSTSPAGGSSRPIGVGSGVQATVVASTSSSSSSAKQLKNNLETSIERLTAVSEQLSYIIQQNHEELTKDDDYYFALSLVPAMRHLSLSRKMYVRSKIQDILFKESEDSTLAKDE

Target ORF information:

RefSeq Version	NM_079023.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant A (BEAF-32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079023.5

ORF Insert Sequence:

ATGCATGTTG AAAAGAAATC AGAGCTTCGT AGCCTACTAA AATCCGGGGA CAAGCGTTGC 
AAACTCGTTG AGCCCAGGAA CACCAAAAGC TGCGTCTGGA GGTTTTTCAA TCTCGTCCAG 
TGCGACGACC ACATAGAGCC GTACGCCTGC TGCAAAACCT GTGGCGATCT GTTATCCTAC 
AGCGGCAAAA CTGGAACCGG ATCCCTGCTA CGGCACCGAT GTCTCCACTC CAGCAGCAGT 
AACGACAAAA CCGTGCGGAT TACCAAGGCC AAGACGCTGA GGGAGCCGCT GCGTGTGGCC 
AAGCCGAAGA TCGAGTCGAA CGTCGCCAAT TATCTGGGCG AGGCGGGCGC CCTGCCGCAA 
AAGTGGGAGG AGTACGAGCA GAACGATGAG ATTAGCGAGG ACATTAAGGA CATCATATAC 
AGCGAGGATC CACTGTGCTA TAGTCCTATA CACGTGATGG ATGATGAGGG CCTGGATCAG 
CCTGAGAAGC AAGTGACCGT GCTAACCCAT TCGACTTCAC CAGCCGGTGG AAGTAGCAGG 
CCCATCGGTG TTGGTTCGGG CGTACAGGCT ACTGTGGTGG CCTCCACGTC CTCCAGCAGC 
AGCTCGGCCA AGCAGCTGAA GAACAACCTG GAGACGTCCA TCGAACGGCT GACCGCTGTC 
AGCGAACAGC TCAGCTACAT CATACAACAG AATCACGAGG AGCTCACCAA GGACGACGAC 
TACTATTTTG CACTTAGCTT GGTGCCCGCA ATGCGCCACC TTTCGCTCAG TCGGAAGATG 
TACGTGCGGT CCAAGATCCA GGATATACTG TTCAAGGAAA GTGAGGACTC AACGCTTGCC 
AAGGATGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEAF-32 ( NM_166069.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725418.1 Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant B (BEAF-32), mRNA.

Price & Availability↑

CloneID	OFa10021
Clone ID Related Accession (Same CDS sequence)	NM_166069.3 , NM_001259399.2
Accession Version	NM_166069.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	boundary element-associated factor of 32kD, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_166069.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCAAGG GTCGTGTTAG CAATGTGTGG CAGCACTACG ATATTAACGA GGAGTGCGAA 
AACTTTGCGA TATGCCGTTA TTGCGGCAAC AATATATCGC GTGGTGGCAT GGCCAGCAAT 
TTGAAGGGCA ACAATACGAC CAATTTGTGG ACGCATCTGC GGCACAAGCA CAGGGACGAG 
GTGCTGGTCA GTCCGGTGCA GCAACGTCCC CAGGCCCGGA TGATCCCAAT CATCAAGAAA 
GACAAAACCG TGCGGATTAC CAAGGCCAAG ACGCTGAGGG AGCCGCTGCG TGTGGCCAAG 
CCGAAGATCG AGTCGAACGT CGCCAATTAT CTGGGCGAGG CGGGCGCCCT GCCGCAAAAG 
TGGGAGGAGT ACGAGCAGAA CGATGAGATT AGCGAGGACA TTAAGGACAT CATATACAGC 
GAGGATCCAC TGTGCTATAG TCCTATACAC GTGATGGATG ATGAGGGCCT GGATCAGCCT 
GAGAAGCAAG TGACCGTGCT AACCCATTCG ACTTCACCAG CCGGTGGAAG TAGCAGGCCC 
ATCGGTGTTG GTTCGGGCGT ACAGGCTACT GTGGTGGCCT CCACGTCCTC CAGCAGCAGC 
TCGGCCAAGC AGCTGAAGAA CAACCTGGAG ACGTCCATCG AACGGCTGAC CGCTGTCAGC 
GAACAGCTCA GCTACATCAT ACAACAGAAT CACGAGGAGC TCACCAAGGA CGACGACTAC 
TATTTTGCAC TTAGCTTGGT GCCCGCAATG CGCCACCTTT CGCTCAGTCG GAAGATGTAC 
GTGCGGTCCA AGATCCAGGA TATACTGTTC AAGGAAAGTG AGGACTCAAC GCTTGCCAAG 
GATGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725418.1
CDS	102..950
Translation	MPKGRVSNVWQHYDINEECENFAICRYCGNNISRGGMASNLKGNNTTNLWTHLRHKHRDEVLVSPVQQRPQARMIPIIKKDKTVRITKAKTLREPLRVAKPKIESNVANYLGEAGALPQKWEEYEQNDEISEDIKDIIYSEDPLCYSPIHVMDDEGLDQPEKQVTVLTHSTSPAGGSSRPIGVGSGVQATVVASTSSSSSSAKQLKNNLETSIERLTAVSEQLSYIIQQNHEELTKDDDYYFALSLVPAMRHLSLSRKMYVRSKIQDILFKESEDSTLAKDE

Target ORF information:

RefSeq Version	NM_166069.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant B (BEAF-32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166069.3

ORF Insert Sequence:

ATGCCCAAGG GTCGTGTTAG CAATGTGTGG CAGCACTACG ATATTAACGA GGAGTGCGAA 
AACTTTGCGA TATGCCGTTA TTGCGGCAAC AATATATCGC GTGGTGGCAT GGCCAGCAAT 
TTGAAGGGCA ACAATACGAC CAATTTGTGG ACGCATCTGC GGCACAAGCA CAGGGACGAG 
GTGCTGGTCA GTCCGGTGCA GCAACGTCCC CAGGCCCGGA TGATCCCAAT CATCAAGAAA 
GACAAAACCG TGCGGATTAC CAAGGCCAAG ACGCTGAGGG AGCCGCTGCG TGTGGCCAAG 
CCGAAGATCG AGTCGAACGT CGCCAATTAT CTGGGCGAGG CGGGCGCCCT GCCGCAAAAG 
TGGGAGGAGT ACGAGCAGAA CGATGAGATT AGCGAGGACA TTAAGGACAT CATATACAGC 
GAGGATCCAC TGTGCTATAG TCCTATACAC GTGATGGATG ATGAGGGCCT GGATCAGCCT 
GAGAAGCAAG TGACCGTGCT AACCCATTCG ACTTCACCAG CCGGTGGAAG TAGCAGGCCC 
ATCGGTGTTG GTTCGGGCGT ACAGGCTACT GTGGTGGCCT CCACGTCCTC CAGCAGCAGC 
TCGGCCAAGC AGCTGAAGAA CAACCTGGAG ACGTCCATCG AACGGCTGAC CGCTGTCAGC 
GAACAGCTCA GCTACATCAT ACAACAGAAT CACGAGGAGC TCACCAAGGA CGACGACTAC 
TATTTTGCAC TTAGCTTGGT GCCCGCAATG CGCCACCTTT CGCTCAGTCG GAAGATGTAC 
GTGCGGTCCA AGATCCAGGA TATACTGTTC AAGGAAAGTG AGGACTCAAC GCTTGCCAAG 
GATGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEAF-32 ( NM_001259399.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246328.1 Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant C (BEAF-32), mRNA.

Price & Availability↑

CloneID	OFa10021
Clone ID Related Accession (Same CDS sequence)	NM_166069.3 , NM_001259399.2
Accession Version	NM_001259399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	boundary element-associated factor of 32kD, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259399.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCAAGG GTCGTGTTAG CAATGTGTGG CAGCACTACG ATATTAACGA GGAGTGCGAA 
AACTTTGCGA TATGCCGTTA TTGCGGCAAC AATATATCGC GTGGTGGCAT GGCCAGCAAT 
TTGAAGGGCA ACAATACGAC CAATTTGTGG ACGCATCTGC GGCACAAGCA CAGGGACGAG 
GTGCTGGTCA GTCCGGTGCA GCAACGTCCC CAGGCCCGGA TGATCCCAAT CATCAAGAAA 
GACAAAACCG TGCGGATTAC CAAGGCCAAG ACGCTGAGGG AGCCGCTGCG TGTGGCCAAG 
CCGAAGATCG AGTCGAACGT CGCCAATTAT CTGGGCGAGG CGGGCGCCCT GCCGCAAAAG 
TGGGAGGAGT ACGAGCAGAA CGATGAGATT AGCGAGGACA TTAAGGACAT CATATACAGC 
GAGGATCCAC TGTGCTATAG TCCTATACAC GTGATGGATG ATGAGGGCCT GGATCAGCCT 
GAGAAGCAAG TGACCGTGCT AACCCATTCG ACTTCACCAG CCGGTGGAAG TAGCAGGCCC 
ATCGGTGTTG GTTCGGGCGT ACAGGCTACT GTGGTGGCCT CCACGTCCTC CAGCAGCAGC 
TCGGCCAAGC AGCTGAAGAA CAACCTGGAG ACGTCCATCG AACGGCTGAC CGCTGTCAGC 
GAACAGCTCA GCTACATCAT ACAACAGAAT CACGAGGAGC TCACCAAGGA CGACGACTAC 
TATTTTGCAC TTAGCTTGGT GCCCGCAATG CGCCACCTTT CGCTCAGTCG GAAGATGTAC 
GTGCGGTCCA AGATCCAGGA TATACTGTTC AAGGAAAGTG AGGACTCAAC GCTTGCCAAG 
GATGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246328.1
CDS	487..1335
Translation	MPKGRVSNVWQHYDINEECENFAICRYCGNNISRGGMASNLKGNNTTNLWTHLRHKHRDEVLVSPVQQRPQARMIPIIKKDKTVRITKAKTLREPLRVAKPKIESNVANYLGEAGALPQKWEEYEQNDEISEDIKDIIYSEDPLCYSPIHVMDDEGLDQPEKQVTVLTHSTSPAGGSSRPIGVGSGVQATVVASTSSSSSSAKQLKNNLETSIERLTAVSEQLSYIIQQNHEELTKDDDYYFALSLVPAMRHLSLSRKMYVRSKIQDILFKESEDSTLAKDE

Target ORF information:

RefSeq Version	NM_001259399.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster boundary element-associated factor of 32kD, transcript variant C (BEAF-32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259399.2

ORF Insert Sequence:

ATGCCCAAGG GTCGTGTTAG CAATGTGTGG CAGCACTACG ATATTAACGA GGAGTGCGAA 
AACTTTGCGA TATGCCGTTA TTGCGGCAAC AATATATCGC GTGGTGGCAT GGCCAGCAAT 
TTGAAGGGCA ACAATACGAC CAATTTGTGG ACGCATCTGC GGCACAAGCA CAGGGACGAG 
GTGCTGGTCA GTCCGGTGCA GCAACGTCCC CAGGCCCGGA TGATCCCAAT CATCAAGAAA 
GACAAAACCG TGCGGATTAC CAAGGCCAAG ACGCTGAGGG AGCCGCTGCG TGTGGCCAAG 
CCGAAGATCG AGTCGAACGT CGCCAATTAT CTGGGCGAGG CGGGCGCCCT GCCGCAAAAG 
TGGGAGGAGT ACGAGCAGAA CGATGAGATT AGCGAGGACA TTAAGGACAT CATATACAGC 
GAGGATCCAC TGTGCTATAG TCCTATACAC GTGATGGATG ATGAGGGCCT GGATCAGCCT 
GAGAAGCAAG TGACCGTGCT AACCCATTCG ACTTCACCAG CCGGTGGAAG TAGCAGGCCC 
ATCGGTGTTG GTTCGGGCGT ACAGGCTACT GTGGTGGCCT CCACGTCCTC CAGCAGCAGC 
TCGGCCAAGC AGCTGAAGAA CAACCTGGAG ACGTCCATCG AACGGCTGAC CGCTGTCAGC 
GAACAGCTCA GCTACATCAT ACAACAGAAT CACGAGGAGC TCACCAAGGA CGACGACTAC 
TATTTTGCAC TTAGCTTGGT GCCCGCAATG CGCCACCTTT CGCTCAGTCG GAAGATGTAC 
GTGCGGTCCA AGATCCAGGA TATACTGTTC AAGGAAAGTG AGGACTCAAC GCTTGCCAAG 
GATGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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BEAF-32 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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