hkb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hkb
Entrez Gene ID	40549
Full Name	huckebein
Synonyms	5953,CG9768,Dmel\CG9768,HKB,Hkb,anon-82B3,gurt
General protein information	Preferred Names huckebein Names CG9768 gene product from transcript CG9768-RA CG9768-PA gurtelchen hkb-PA huckbein hucklebein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 82A4-82A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_079497.4	NP_524221.1	huckebein

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004517	XP_313817.5
Drosophila melanogaster (fruit fly)	hkb	NP_524221.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Huckebein is part of a combinatorial repression code in the anterior blastoderm.
Title: Huckebein is part of a combinatorial repression code in the anterior blastoderm.

hkb(gurt) mutant embryos reveal that the adjacent endoderm plays a critical role in the later stages of visceral muscle development
Title: Fusion of circular and longitudinal muscles in Drosophila is independent of the endoderm but further visceral muscle differentiation requires a close contact between mesoderm and endoderm.

Huckebein does not activate transcription; rather, it physically interacts with Glide/Gcm thereby triggering its autoregulation.
Title: Huckebein-mediated autoregulation of Glide/Gcm triggers glia specification.

hkb plays a role in salivary tube development in Drosophila
Title: Epithelial tube morphology is determined by the polarized growth and delivery of apical membrane.

The following hkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03817	NM_079497.4 Latest version!	Drosophila melanogaster huckebein (hkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hkb ( NM_079497.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524221.1 Drosophila melanogaster huckebein (hkb), mRNA.

Price & Availability↑

CloneID	OFa03817
Clone ID Related Accession (Same CDS sequence)	NM_079497.4
Accession Version	NM_079497.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	huckebein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079497.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACGA TTAACCTGCA TCCCCCGCAA ACCTACTCGC GACTTTTCCG ACCCTGGGAT 
ACTCAGCGCC AGGCGGCGAC CTCGGCGGCA CCCAGGACAG TGAAGCAGGA GCCGGAGGCG 
ATTACCATCA AGACGGAGCT GGCCAGCAGC AGTTTCGGCC ACGACTGCGA CGGAGACTTC 
TCCTCCGCAT CCTCCTCTGC GTCAAGCAGC AGCAACAGCA GCAAGTCCTG CTACGAGGAG 
GCCCTGAACC ACAGCAGCTA CACCCGCACT TCCACCCCGC TGCTGGACGC CGCCCCGCAC 
CCCGTCTTTT CACACCCGCA GTCGTCGCCG TTGGACACGC ACGCCGCCGC CACCGCATCC 
TTGGCTCCGC CAAACCAGCA CGCTCCATTC CTGTCGGCCG CCAGCGATCT CTACTACGCT 
GCCGCCGCCG CAGCAGCCGC TGCTGCCTCC ACGCCCACTG CCGTCCCGGG CTTCGGCATG 
GACCCGTTCA CTATGGGCCT GATGGAGCAA GAGTACGCCC GCGTGATGGC CGAGGACGCA 
CAGTTAAAGG CGCTCAACAG CCGCAAGCAG CGGCCGAAGA AGTTCAAGTG CCCCAACTGC 
GACGTGGCCT TCTCCAACAA CGGCCAGCTG AAGGGCCACA TCCGCATCCA CACCGGCGAA 
CGCCCATTTA AGTGCGACGT GAACACATGC GGCAAGACCT TCACCCGCAA TGAGGAACTG 
ACGCGTCACA AGCGAATCCA CACCGGACTG CGGCCGTATC CTTGCAGTGC CTGCGGAAAG 
AAGTTTGGCC GACGCGACCA CTTAAAGAAG CACATGAAGA CGCACATGCC GCAGGAGCGC 
CAGCTGGGCC CGTCCATCTT CGTGCCCATG TACTCATACC TTTATGGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524221.1
CDS	144..1037
Translation	MSTINLHPPQTYSRLFRPWDTQRQAATSAAPRTVKQEPEAITIKTELASSSFGHDCDGDFSSASSSASSSSNSSKSCYEEALNHSSYTRTSTPLLDAAPHPVFSHPQSSPLDTHAAATASLAPPNQHAPFLSAASDLYYAAAAAAAAAASTPTAVPGFGMDPFTMGLMEQEYARVMAEDAQLKALNSRKQRPKKFKCPNCDVAFSNNGQLKGHIRIHTGERPFKCDVNTCGKTFTRNEELTRHKRIHTGLRPYPCSACGKKFGRRDHLKKHMKTHMPQERQLGPSIFVPMYSYLYGY

Target ORF information:

RefSeq Version	NM_079497.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster huckebein (hkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079497.4

ORF Insert Sequence:

ATGTCGACGA TTAACCTGCA TCCCCCGCAA ACCTACTCGC GACTTTTCCG ACCCTGGGAT 
ACTCAGCGCC AGGCGGCGAC CTCGGCGGCA CCCAGGACAG TGAAGCAGGA GCCGGAGGCG 
ATTACCATCA AGACGGAGCT GGCCAGCAGC AGTTTCGGCC ACGACTGCGA CGGAGACTTC 
TCCTCCGCAT CCTCCTCTGC GTCAAGCAGC AGCAACAGCA GCAAGTCCTG CTACGAGGAG 
GCCCTGAACC ACAGCAGCTA CACCCGCACT TCCACCCCGC TGCTGGACGC CGCCCCGCAC 
CCCGTCTTTT CACACCCGCA GTCGTCGCCG TTGGACACGC ACGCCGCCGC CACCGCATCC 
TTGGCTCCGC CAAACCAGCA CGCTCCATTC CTGTCGGCCG CCAGCGATCT CTACTACGCT 
GCCGCCGCCG CAGCAGCCGC TGCTGCCTCC ACGCCCACTG CCGTCCCGGG CTTCGGCATG 
GACCCGTTCA CTATGGGCCT GATGGAGCAA GAGTACGCCC GCGTGATGGC CGAGGACGCA 
CAGTTAAAGG CGCTCAACAG CCGCAAGCAG CGGCCGAAGA AGTTCAAGTG CCCCAACTGC 
GACGTGGCCT TCTCCAACAA CGGCCAGCTG AAGGGCCACA TCCGCATCCA CACCGGCGAA 
CGCCCATTTA AGTGCGACGT GAACACATGC GGCAAGACCT TCACCCGCAA TGAGGAACTG 
ACGCGTCACA AGCGAATCCA CACCGGACTG CGGCCGTATC CTTGCAGTGC CTGCGGAAAG 
AAGTTTGGCC GACGCGACCA CTTAAAGAAG CACATGAAGA CGCACATGCC GCAGGAGCGC 
CAGCTGGGCC CGTCCATCTT CGTGCCCATG TACTCATACC TTTATGGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hkb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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