PR-Set7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PR-Set7
Entrez Gene ID	41743
Full Name	PR/SET domain containing protein 7
Synonyms	CG3307,Dmel\CG3307,PR-SET7,PR/SET07,PRSET7,Pr-set-7,PrSet7,SET8,Set7,Set8,dPR-Set7,dSET8,dSet8,l(3)neo41,pr-Set7,pr-set7
General protein information	Preferred Names PR/SET domain containing protein 7 Names CG3307 gene product from transcript CG3307-RB CG3307-PA CG3307-PB CG3307-PD PR-Set7-PA PR-Set7-PB PR-Set7-PD SET domain containing 8 pr-set7
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88B3-88B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260171.2	NP_001247100.1	PR/SET domain containing protein 7, isoform D
NM_142097.4	NP_650354.1	PR/SET domain containing protein 7, isoform A
NM_169577.3	NP_731900.1	PR/SET domain containing protein 7, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

coincident with the ATR-dependent cell cycle arrest, increased DNA damage is specifically limited to late replicating regions of the Drosophila genome, suggesting that PR-Set7-mediated monomethylation of H4K20 is critical for maintaining the genomic integrity of late replicating domains
Title: Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila.

crystal structure reveals the basis of the specificity of the enzyme for histone H4-K20
Title: Specificity and mechanism of the histone methyltransferase Pr-Set7.

Results support the hypothesis that methylation of H4 lysine 20 by PR/Set7 maintains silent chromatin, in part, by precluding neighboring acetylation on the H4 tail
Title: PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin.

The following PR-Set7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PR-Set7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04860	NM_001260171.2 Latest version!	Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa04860	NM_142097.4 Latest version!	Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa04860	NM_169577.3 Latest version!	Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PR-Set7 ( NM_001260171.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247100.1 Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa04860
Clone ID Related Accession (Same CDS sequence)	NM_001260171.2 , NM_142097.4 , NM_169577.3
Accession Version	NM_001260171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PR/SET domain containing protein 7, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260171.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAATGG TGCGAAGACG ACAACGGCCC GCCAAGGAAG CCGCATCCTC ATCCTCTGGT 
GGAGCTTCCT CGGGATCGGG AATTCCTGTT GACCAGGCAC TGCCACTGAA TGTGGCGGGA 
AATCTGCTGG AGGATCAGTA TTTTGCCAGC CCCAAGCGGA AAGACTGCCG TTTGATGAAG 
GTCACTCAAA ATGGCCAACT GCCGGAAGCC ACAATGATGG CACACAACAA AGACAACAAA 
GCGGGTCGCA CAATAGGAGT TCCCTTGGCC ACGCGCTCAC AAACTCGCAC CATTGAGAAC 
TTCTTCAAAG CCAATGCTGC CGCTAAGGAT TCCCAAAAGA CAATACACAC AGAGGAGCAG 
TTGAATCTAG GAAATCAGGA ACTGAAACTG GACGATGAGG AGCTAAATGG ACAGATAAAA 
CTAGATGACG AGGTGCTAAA GCTGGCAGAC AAGCAGATAA ATGAAAACCT TCCCTTTGCC 
GACGAAGTAG ATGCCAAGGC AGAACAAAAG CTTATGGATG AGGAACTGCA GCAGGTGGTG 
GAGGAACTTC TGTTCGATGG CAGTTCCAGA GCGTCCTCCA ATTCGCCCTT TTATCAGCAT 
GATATGGATG TCATGCAGGA GATCCAGCAG ACTCCAGAAA TTCCACACAT CAAAAAGGTC 
ACAGAGCCGC TCGAGGGACT GGGCTCTCTG GCCGACTTCC AGACACATCG CTCTGCGCTG 
AGGGACAGCC ACAGTTCCAC GCACAGCAGC AGCACCGACA ACATCTTCCT GCAGGAGCCG 
GTACTGACCC TGGACATTGA TCGCACGCCC ACCAAAGCCT CCAGCATAAA GATCAATCGG 
AGCTTCGAAC TGGCCGGAGC CGTATTCTCA TCACCTCCGT CGGTGCTAAA CGCCTGTCTC 
AATGGACGCT TCAATCAAAT AGTAAGCCTG AATGGACAAA AGGAGGCGCT GGACTTGCCG 
CACTTCGATT TGGATCAACA TGACAGTAGT TCTTGCGACA GCGGAGTAGC CTGTGGTCTC 
ACTGCCAATA CAGAATCGCC AGCGGGGCAA CCCCGACGCA GAAAACCAGC CACACCGCAT 
CGCATCCTCT GCCCCTCGCC AATCAAAACG GCTTTGAAGG TAACTGGAGG GATTTGCAAG 
GTCGGATCTG CGGATCCATT GTCGCCGCGC AAGTCTCCTC GAAAACTGCC GACCACAACA 
GCAGCGGTGG CAGCCTGCAA GTCGCGTCGA AGACTGAACC AGCCAAAGCC ACAAGCGCCT 
TACCAGCCAC AGCTACAGAA ACCACCGTCA CAACAGCAGC AGCAGCAGCA GGATGATATC 
GTCGTGGTGC TAGACGACGA CGATGATGAA GGAGATGACG AGGACGACGT TCGAGCCCTG 
ATCAAGGCTG CCGAGGAGCG TGAAAACCAG AACAAAGCAC CAGCCACAGC GAACAGCAAC 
AAAGCAGGCA TGAAAACGAT GCTCAAGCCT GCGCCCGTGA AATCAAAGAC CAAAAGCAAG 
GGGCCAACGA AGGGACAACC GCCGCTGCCC TTGGCTGCCA CCAACGGCAA TCGTGAGATG 
ACGGACTTCT TCCCGGTGCG AAGGAGTGTG CGCAAGACTA AGACGGCCGT CAAGGAGGAA 
TGGATGCGAG GCTTGGAGCA AGCAGTGCTG GAAGAGCGCT GCGATGGTCT CCAAGTACGC 
CACTTTATGG GCAAGGGAAG AGGCGTAGTC GCCGATCGGC CCTTTAAACG CAACGAATTT 
GTGGTTGAGT ATGTGGGAGA TCTCATATCT ATAGGCGAAG CCGCGGAACG GGAGAAACGC 
TATGCGTTGG ACGAGAATGC TGGGTGCTAT ATGTACTACT TCAAGCACAA GTCCCAGCAG 
TACTGCATCG ATGCAACCGT TGACACCGGC AAGCTGGGGC GCCTCATCAA TCACTCGCGG 
GCTGGTAACC TAATGACCAA AGTAGTACTT ATCAAACAGC GGCCGCATCT CGTACTCCTG 
GCCAAGGACG ACATCGAGCC GGGCGAGGAA CTGACCTATG ACTACGGGGA CCGATCGAAG 
GAGTCACTAT TGCACCATCC TTGGTTGGCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247100.1
CDS	166..2241
Translation	MIMVRRRQRPAKEAASSSSGGASSGSGIPVDQALPLNVAGNLLEDQYFASPKRKDCRLMKVTQNGQLPEATMMAHNKDNKAGRTIGVPLATRSQTRTIENFFKANAAAKDSQKTIHTEEQLNLGNQELKLDDEELNGQIKLDDEVLKLADKQINENLPFADEVDAKAEQKLMDEELQQVVEELLFDGSSRASSNSPFYQHDMDVMQEIQQTPEIPHIKKVTEPLEGLGSLADFQTHRSALRDSHSSTHSSSTDNIFLQEPVLTLDIDRTPTKASSIKINRSFELAGAVFSSPPSVLNACLNGRFNQIVSLNGQKEALDLPHFDLDQHDSSSCDSGVACGLTANTESPAGQPRRRKPATPHRILCPSPIKTALKVTGGICKVGSADPLSPRKSPRKLPTTTAAVAACKSRRRLNQPKPQAPYQPQLQKPPSQQQQQQQDDIVVVLDDDDDEGDDEDDVRALIKAAEERENQNKAPATANSNKAGMKTMLKPAPVKSKTKSKGPTKGQPPLPLAATNGNREMTDFFPVRRSVRKTKTAVKEEWMRGLEQAVLEERCDGLQVRHFMGKGRGVVADRPFKRNEFVVEYVGDLISIGEAAEREKRYALDENAGCYMYYFKHKSQQYCIDATVDTGKLGRLINHSRAGNLMTKVVLIKQRPHLVLLAKDDIEPGEELTYDYGDRSKESLLHHPWLAF

Target ORF information:

RefSeq Version	NM_001260171.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260171.2

ORF Insert Sequence:

ATGATAATGG TGCGAAGACG ACAACGGCCC GCCAAGGAAG CCGCATCCTC ATCCTCTGGT 
GGAGCTTCCT CGGGATCGGG AATTCCTGTT GACCAGGCAC TGCCACTGAA TGTGGCGGGA 
AATCTGCTGG AGGATCAGTA TTTTGCCAGC CCCAAGCGGA AAGACTGCCG TTTGATGAAG 
GTCACTCAAA ATGGCCAACT GCCGGAAGCC ACAATGATGG CACACAACAA AGACAACAAA 
GCGGGTCGCA CAATAGGAGT TCCCTTGGCC ACGCGCTCAC AAACTCGCAC CATTGAGAAC 
TTCTTCAAAG CCAATGCTGC CGCTAAGGAT TCCCAAAAGA CAATACACAC AGAGGAGCAG 
TTGAATCTAG GAAATCAGGA ACTGAAACTG GACGATGAGG AGCTAAATGG ACAGATAAAA 
CTAGATGACG AGGTGCTAAA GCTGGCAGAC AAGCAGATAA ATGAAAACCT TCCCTTTGCC 
GACGAAGTAG ATGCCAAGGC AGAACAAAAG CTTATGGATG AGGAACTGCA GCAGGTGGTG 
GAGGAACTTC TGTTCGATGG CAGTTCCAGA GCGTCCTCCA ATTCGCCCTT TTATCAGCAT 
GATATGGATG TCATGCAGGA GATCCAGCAG ACTCCAGAAA TTCCACACAT CAAAAAGGTC 
ACAGAGCCGC TCGAGGGACT GGGCTCTCTG GCCGACTTCC AGACACATCG CTCTGCGCTG 
AGGGACAGCC ACAGTTCCAC GCACAGCAGC AGCACCGACA ACATCTTCCT GCAGGAGCCG 
GTACTGACCC TGGACATTGA TCGCACGCCC ACCAAAGCCT CCAGCATAAA GATCAATCGG 
AGCTTCGAAC TGGCCGGAGC CGTATTCTCA TCACCTCCGT CGGTGCTAAA CGCCTGTCTC 
AATGGACGCT TCAATCAAAT AGTAAGCCTG AATGGACAAA AGGAGGCGCT GGACTTGCCG 
CACTTCGATT TGGATCAACA TGACAGTAGT TCTTGCGACA GCGGAGTAGC CTGTGGTCTC 
ACTGCCAATA CAGAATCGCC AGCGGGGCAA CCCCGACGCA GAAAACCAGC CACACCGCAT 
CGCATCCTCT GCCCCTCGCC AATCAAAACG GCTTTGAAGG TAACTGGAGG GATTTGCAAG 
GTCGGATCTG CGGATCCATT GTCGCCGCGC AAGTCTCCTC GAAAACTGCC GACCACAACA 
GCAGCGGTGG CAGCCTGCAA GTCGCGTCGA AGACTGAACC AGCCAAAGCC ACAAGCGCCT 
TACCAGCCAC AGCTACAGAA ACCACCGTCA CAACAGCAGC AGCAGCAGCA GGATGATATC 
GTCGTGGTGC TAGACGACGA CGATGATGAA GGAGATGACG AGGACGACGT TCGAGCCCTG 
ATCAAGGCTG CCGAGGAGCG TGAAAACCAG AACAAAGCAC CAGCCACAGC GAACAGCAAC 
AAAGCAGGCA TGAAAACGAT GCTCAAGCCT GCGCCCGTGA AATCAAAGAC CAAAAGCAAG 
GGGCCAACGA AGGGACAACC GCCGCTGCCC TTGGCTGCCA CCAACGGCAA TCGTGAGATG 
ACGGACTTCT TCCCGGTGCG AAGGAGTGTG CGCAAGACTA AGACGGCCGT CAAGGAGGAA 
TGGATGCGAG GCTTGGAGCA AGCAGTGCTG GAAGAGCGCT GCGATGGTCT CCAAGTACGC 
CACTTTATGG GCAAGGGAAG AGGCGTAGTC GCCGATCGGC CCTTTAAACG CAACGAATTT 
GTGGTTGAGT ATGTGGGAGA TCTCATATCT ATAGGCGAAG CCGCGGAACG GGAGAAACGC 
TATGCGTTGG ACGAGAATGC TGGGTGCTAT ATGTACTACT TCAAGCACAA GTCCCAGCAG 
TACTGCATCG ATGCAACCGT TGACACCGGC AAGCTGGGGC GCCTCATCAA TCACTCGCGG 
GCTGGTAACC TAATGACCAA AGTAGTACTT ATCAAACAGC GGCCGCATCT CGTACTCCTG 
GCCAAGGACG ACATCGAGCC GGGCGAGGAA CTGACCTATG ACTACGGGGA CCGATCGAAG 
GAGTCACTAT TGCACCATCC TTGGTTGGCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PR-Set7 ( NM_142097.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650354.1 Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04860
Clone ID Related Accession (Same CDS sequence)	NM_001260171.2 , NM_142097.4 , NM_169577.3
Accession Version	NM_142097.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PR/SET domain containing protein 7, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142097.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAATGG TGCGAAGACG ACAACGGCCC GCCAAGGAAG CCGCATCCTC ATCCTCTGGT 
GGAGCTTCCT CGGGATCGGG AATTCCTGTT GACCAGGCAC TGCCACTGAA TGTGGCGGGA 
AATCTGCTGG AGGATCAGTA TTTTGCCAGC CCCAAGCGGA AAGACTGCCG TTTGATGAAG 
GTCACTCAAA ATGGCCAACT GCCGGAAGCC ACAATGATGG CACACAACAA AGACAACAAA 
GCGGGTCGCA CAATAGGAGT TCCCTTGGCC ACGCGCTCAC AAACTCGCAC CATTGAGAAC 
TTCTTCAAAG CCAATGCTGC CGCTAAGGAT TCCCAAAAGA CAATACACAC AGAGGAGCAG 
TTGAATCTAG GAAATCAGGA ACTGAAACTG GACGATGAGG AGCTAAATGG ACAGATAAAA 
CTAGATGACG AGGTGCTAAA GCTGGCAGAC AAGCAGATAA ATGAAAACCT TCCCTTTGCC 
GACGAAGTAG ATGCCAAGGC AGAACAAAAG CTTATGGATG AGGAACTGCA GCAGGTGGTG 
GAGGAACTTC TGTTCGATGG CAGTTCCAGA GCGTCCTCCA ATTCGCCCTT TTATCAGCAT 
GATATGGATG TCATGCAGGA GATCCAGCAG ACTCCAGAAA TTCCACACAT CAAAAAGGTC 
ACAGAGCCGC TCGAGGGACT GGGCTCTCTG GCCGACTTCC AGACACATCG CTCTGCGCTG 
AGGGACAGCC ACAGTTCCAC GCACAGCAGC AGCACCGACA ACATCTTCCT GCAGGAGCCG 
GTACTGACCC TGGACATTGA TCGCACGCCC ACCAAAGCCT CCAGCATAAA GATCAATCGG 
AGCTTCGAAC TGGCCGGAGC CGTATTCTCA TCACCTCCGT CGGTGCTAAA CGCCTGTCTC 
AATGGACGCT TCAATCAAAT AGTAAGCCTG AATGGACAAA AGGAGGCGCT GGACTTGCCG 
CACTTCGATT TGGATCAACA TGACAGTAGT TCTTGCGACA GCGGAGTAGC CTGTGGTCTC 
ACTGCCAATA CAGAATCGCC AGCGGGGCAA CCCCGACGCA GAAAACCAGC CACACCGCAT 
CGCATCCTCT GCCCCTCGCC AATCAAAACG GCTTTGAAGG TAACTGGAGG GATTTGCAAG 
GTCGGATCTG CGGATCCATT GTCGCCGCGC AAGTCTCCTC GAAAACTGCC GACCACAACA 
GCAGCGGTGG CAGCCTGCAA GTCGCGTCGA AGACTGAACC AGCCAAAGCC ACAAGCGCCT 
TACCAGCCAC AGCTACAGAA ACCACCGTCA CAACAGCAGC AGCAGCAGCA GGATGATATC 
GTCGTGGTGC TAGACGACGA CGATGATGAA GGAGATGACG AGGACGACGT TCGAGCCCTG 
ATCAAGGCTG CCGAGGAGCG TGAAAACCAG AACAAAGCAC CAGCCACAGC GAACAGCAAC 
AAAGCAGGCA TGAAAACGAT GCTCAAGCCT GCGCCCGTGA AATCAAAGAC CAAAAGCAAG 
GGGCCAACGA AGGGACAACC GCCGCTGCCC TTGGCTGCCA CCAACGGCAA TCGTGAGATG 
ACGGACTTCT TCCCGGTGCG AAGGAGTGTG CGCAAGACTA AGACGGCCGT CAAGGAGGAA 
TGGATGCGAG GCTTGGAGCA AGCAGTGCTG GAAGAGCGCT GCGATGGTCT CCAAGTACGC 
CACTTTATGG GCAAGGGAAG AGGCGTAGTC GCCGATCGGC CCTTTAAACG CAACGAATTT 
GTGGTTGAGT ATGTGGGAGA TCTCATATCT ATAGGCGAAG CCGCGGAACG GGAGAAACGC 
TATGCGTTGG ACGAGAATGC TGGGTGCTAT ATGTACTACT TCAAGCACAA GTCCCAGCAG 
TACTGCATCG ATGCAACCGT TGACACCGGC AAGCTGGGGC GCCTCATCAA TCACTCGCGG 
GCTGGTAACC TAATGACCAA AGTAGTACTT ATCAAACAGC GGCCGCATCT CGTACTCCTG 
GCCAAGGACG ACATCGAGCC GGGCGAGGAA CTGACCTATG ACTACGGGGA CCGATCGAAG 
GAGTCACTAT TGCACCATCC TTGGTTGGCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650354.1
CDS	302..2377
Translation	MIMVRRRQRPAKEAASSSSGGASSGSGIPVDQALPLNVAGNLLEDQYFASPKRKDCRLMKVTQNGQLPEATMMAHNKDNKAGRTIGVPLATRSQTRTIENFFKANAAAKDSQKTIHTEEQLNLGNQELKLDDEELNGQIKLDDEVLKLADKQINENLPFADEVDAKAEQKLMDEELQQVVEELLFDGSSRASSNSPFYQHDMDVMQEIQQTPEIPHIKKVTEPLEGLGSLADFQTHRSALRDSHSSTHSSSTDNIFLQEPVLTLDIDRTPTKASSIKINRSFELAGAVFSSPPSVLNACLNGRFNQIVSLNGQKEALDLPHFDLDQHDSSSCDSGVACGLTANTESPAGQPRRRKPATPHRILCPSPIKTALKVTGGICKVGSADPLSPRKSPRKLPTTTAAVAACKSRRRLNQPKPQAPYQPQLQKPPSQQQQQQQDDIVVVLDDDDDEGDDEDDVRALIKAAEERENQNKAPATANSNKAGMKTMLKPAPVKSKTKSKGPTKGQPPLPLAATNGNREMTDFFPVRRSVRKTKTAVKEEWMRGLEQAVLEERCDGLQVRHFMGKGRGVVADRPFKRNEFVVEYVGDLISIGEAAEREKRYALDENAGCYMYYFKHKSQQYCIDATVDTGKLGRLINHSRAGNLMTKVVLIKQRPHLVLLAKDDIEPGEELTYDYGDRSKESLLHHPWLAF

Target ORF information:

RefSeq Version	NM_142097.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142097.4

ORF Insert Sequence:

ATGATAATGG TGCGAAGACG ACAACGGCCC GCCAAGGAAG CCGCATCCTC ATCCTCTGGT 
GGAGCTTCCT CGGGATCGGG AATTCCTGTT GACCAGGCAC TGCCACTGAA TGTGGCGGGA 
AATCTGCTGG AGGATCAGTA TTTTGCCAGC CCCAAGCGGA AAGACTGCCG TTTGATGAAG 
GTCACTCAAA ATGGCCAACT GCCGGAAGCC ACAATGATGG CACACAACAA AGACAACAAA 
GCGGGTCGCA CAATAGGAGT TCCCTTGGCC ACGCGCTCAC AAACTCGCAC CATTGAGAAC 
TTCTTCAAAG CCAATGCTGC CGCTAAGGAT TCCCAAAAGA CAATACACAC AGAGGAGCAG 
TTGAATCTAG GAAATCAGGA ACTGAAACTG GACGATGAGG AGCTAAATGG ACAGATAAAA 
CTAGATGACG AGGTGCTAAA GCTGGCAGAC AAGCAGATAA ATGAAAACCT TCCCTTTGCC 
GACGAAGTAG ATGCCAAGGC AGAACAAAAG CTTATGGATG AGGAACTGCA GCAGGTGGTG 
GAGGAACTTC TGTTCGATGG CAGTTCCAGA GCGTCCTCCA ATTCGCCCTT TTATCAGCAT 
GATATGGATG TCATGCAGGA GATCCAGCAG ACTCCAGAAA TTCCACACAT CAAAAAGGTC 
ACAGAGCCGC TCGAGGGACT GGGCTCTCTG GCCGACTTCC AGACACATCG CTCTGCGCTG 
AGGGACAGCC ACAGTTCCAC GCACAGCAGC AGCACCGACA ACATCTTCCT GCAGGAGCCG 
GTACTGACCC TGGACATTGA TCGCACGCCC ACCAAAGCCT CCAGCATAAA GATCAATCGG 
AGCTTCGAAC TGGCCGGAGC CGTATTCTCA TCACCTCCGT CGGTGCTAAA CGCCTGTCTC 
AATGGACGCT TCAATCAAAT AGTAAGCCTG AATGGACAAA AGGAGGCGCT GGACTTGCCG 
CACTTCGATT TGGATCAACA TGACAGTAGT TCTTGCGACA GCGGAGTAGC CTGTGGTCTC 
ACTGCCAATA CAGAATCGCC AGCGGGGCAA CCCCGACGCA GAAAACCAGC CACACCGCAT 
CGCATCCTCT GCCCCTCGCC AATCAAAACG GCTTTGAAGG TAACTGGAGG GATTTGCAAG 
GTCGGATCTG CGGATCCATT GTCGCCGCGC AAGTCTCCTC GAAAACTGCC GACCACAACA 
GCAGCGGTGG CAGCCTGCAA GTCGCGTCGA AGACTGAACC AGCCAAAGCC ACAAGCGCCT 
TACCAGCCAC AGCTACAGAA ACCACCGTCA CAACAGCAGC AGCAGCAGCA GGATGATATC 
GTCGTGGTGC TAGACGACGA CGATGATGAA GGAGATGACG AGGACGACGT TCGAGCCCTG 
ATCAAGGCTG CCGAGGAGCG TGAAAACCAG AACAAAGCAC CAGCCACAGC GAACAGCAAC 
AAAGCAGGCA TGAAAACGAT GCTCAAGCCT GCGCCCGTGA AATCAAAGAC CAAAAGCAAG 
GGGCCAACGA AGGGACAACC GCCGCTGCCC TTGGCTGCCA CCAACGGCAA TCGTGAGATG 
ACGGACTTCT TCCCGGTGCG AAGGAGTGTG CGCAAGACTA AGACGGCCGT CAAGGAGGAA 
TGGATGCGAG GCTTGGAGCA AGCAGTGCTG GAAGAGCGCT GCGATGGTCT CCAAGTACGC 
CACTTTATGG GCAAGGGAAG AGGCGTAGTC GCCGATCGGC CCTTTAAACG CAACGAATTT 
GTGGTTGAGT ATGTGGGAGA TCTCATATCT ATAGGCGAAG CCGCGGAACG GGAGAAACGC 
TATGCGTTGG ACGAGAATGC TGGGTGCTAT ATGTACTACT TCAAGCACAA GTCCCAGCAG 
TACTGCATCG ATGCAACCGT TGACACCGGC AAGCTGGGGC GCCTCATCAA TCACTCGCGG 
GCTGGTAACC TAATGACCAA AGTAGTACTT ATCAAACAGC GGCCGCATCT CGTACTCCTG 
GCCAAGGACG ACATCGAGCC GGGCGAGGAA CTGACCTATG ACTACGGGGA CCGATCGAAG 
GAGTCACTAT TGCACCATCC TTGGTTGGCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PR-Set7 ( NM_169577.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731900.1 Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04860
Clone ID Related Accession (Same CDS sequence)	NM_001260171.2 , NM_142097.4 , NM_169577.3
Accession Version	NM_169577.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PR/SET domain containing protein 7, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169577.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAATGG TGCGAAGACG ACAACGGCCC GCCAAGGAAG CCGCATCCTC ATCCTCTGGT 
GGAGCTTCCT CGGGATCGGG AATTCCTGTT GACCAGGCAC TGCCACTGAA TGTGGCGGGA 
AATCTGCTGG AGGATCAGTA TTTTGCCAGC CCCAAGCGGA AAGACTGCCG TTTGATGAAG 
GTCACTCAAA ATGGCCAACT GCCGGAAGCC ACAATGATGG CACACAACAA AGACAACAAA 
GCGGGTCGCA CAATAGGAGT TCCCTTGGCC ACGCGCTCAC AAACTCGCAC CATTGAGAAC 
TTCTTCAAAG CCAATGCTGC CGCTAAGGAT TCCCAAAAGA CAATACACAC AGAGGAGCAG 
TTGAATCTAG GAAATCAGGA ACTGAAACTG GACGATGAGG AGCTAAATGG ACAGATAAAA 
CTAGATGACG AGGTGCTAAA GCTGGCAGAC AAGCAGATAA ATGAAAACCT TCCCTTTGCC 
GACGAAGTAG ATGCCAAGGC AGAACAAAAG CTTATGGATG AGGAACTGCA GCAGGTGGTG 
GAGGAACTTC TGTTCGATGG CAGTTCCAGA GCGTCCTCCA ATTCGCCCTT TTATCAGCAT 
GATATGGATG TCATGCAGGA GATCCAGCAG ACTCCAGAAA TTCCACACAT CAAAAAGGTC 
ACAGAGCCGC TCGAGGGACT GGGCTCTCTG GCCGACTTCC AGACACATCG CTCTGCGCTG 
AGGGACAGCC ACAGTTCCAC GCACAGCAGC AGCACCGACA ACATCTTCCT GCAGGAGCCG 
GTACTGACCC TGGACATTGA TCGCACGCCC ACCAAAGCCT CCAGCATAAA GATCAATCGG 
AGCTTCGAAC TGGCCGGAGC CGTATTCTCA TCACCTCCGT CGGTGCTAAA CGCCTGTCTC 
AATGGACGCT TCAATCAAAT AGTAAGCCTG AATGGACAAA AGGAGGCGCT GGACTTGCCG 
CACTTCGATT TGGATCAACA TGACAGTAGT TCTTGCGACA GCGGAGTAGC CTGTGGTCTC 
ACTGCCAATA CAGAATCGCC AGCGGGGCAA CCCCGACGCA GAAAACCAGC CACACCGCAT 
CGCATCCTCT GCCCCTCGCC AATCAAAACG GCTTTGAAGG TAACTGGAGG GATTTGCAAG 
GTCGGATCTG CGGATCCATT GTCGCCGCGC AAGTCTCCTC GAAAACTGCC GACCACAACA 
GCAGCGGTGG CAGCCTGCAA GTCGCGTCGA AGACTGAACC AGCCAAAGCC ACAAGCGCCT 
TACCAGCCAC AGCTACAGAA ACCACCGTCA CAACAGCAGC AGCAGCAGCA GGATGATATC 
GTCGTGGTGC TAGACGACGA CGATGATGAA GGAGATGACG AGGACGACGT TCGAGCCCTG 
ATCAAGGCTG CCGAGGAGCG TGAAAACCAG AACAAAGCAC CAGCCACAGC GAACAGCAAC 
AAAGCAGGCA TGAAAACGAT GCTCAAGCCT GCGCCCGTGA AATCAAAGAC CAAAAGCAAG 
GGGCCAACGA AGGGACAACC GCCGCTGCCC TTGGCTGCCA CCAACGGCAA TCGTGAGATG 
ACGGACTTCT TCCCGGTGCG AAGGAGTGTG CGCAAGACTA AGACGGCCGT CAAGGAGGAA 
TGGATGCGAG GCTTGGAGCA AGCAGTGCTG GAAGAGCGCT GCGATGGTCT CCAAGTACGC 
CACTTTATGG GCAAGGGAAG AGGCGTAGTC GCCGATCGGC CCTTTAAACG CAACGAATTT 
GTGGTTGAGT ATGTGGGAGA TCTCATATCT ATAGGCGAAG CCGCGGAACG GGAGAAACGC 
TATGCGTTGG ACGAGAATGC TGGGTGCTAT ATGTACTACT TCAAGCACAA GTCCCAGCAG 
TACTGCATCG ATGCAACCGT TGACACCGGC AAGCTGGGGC GCCTCATCAA TCACTCGCGG 
GCTGGTAACC TAATGACCAA AGTAGTACTT ATCAAACAGC GGCCGCATCT CGTACTCCTG 
GCCAAGGACG ACATCGAGCC GGGCGAGGAA CTGACCTATG ACTACGGGGA CCGATCGAAG 
GAGTCACTAT TGCACCATCC TTGGTTGGCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731900.1
CDS	372..2447
Translation	MIMVRRRQRPAKEAASSSSGGASSGSGIPVDQALPLNVAGNLLEDQYFASPKRKDCRLMKVTQNGQLPEATMMAHNKDNKAGRTIGVPLATRSQTRTIENFFKANAAAKDSQKTIHTEEQLNLGNQELKLDDEELNGQIKLDDEVLKLADKQINENLPFADEVDAKAEQKLMDEELQQVVEELLFDGSSRASSNSPFYQHDMDVMQEIQQTPEIPHIKKVTEPLEGLGSLADFQTHRSALRDSHSSTHSSSTDNIFLQEPVLTLDIDRTPTKASSIKINRSFELAGAVFSSPPSVLNACLNGRFNQIVSLNGQKEALDLPHFDLDQHDSSSCDSGVACGLTANTESPAGQPRRRKPATPHRILCPSPIKTALKVTGGICKVGSADPLSPRKSPRKLPTTTAAVAACKSRRRLNQPKPQAPYQPQLQKPPSQQQQQQQDDIVVVLDDDDDEGDDEDDVRALIKAAEERENQNKAPATANSNKAGMKTMLKPAPVKSKTKSKGPTKGQPPLPLAATNGNREMTDFFPVRRSVRKTKTAVKEEWMRGLEQAVLEERCDGLQVRHFMGKGRGVVADRPFKRNEFVVEYVGDLISIGEAAEREKRYALDENAGCYMYYFKHKSQQYCIDATVDTGKLGRLINHSRAGNLMTKVVLIKQRPHLVLLAKDDIEPGEELTYDYGDRSKESLLHHPWLAF

Target ORF information:

RefSeq Version	NM_169577.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PR/SET domain containing protein 7 (PR-Set7), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169577.3

ORF Insert Sequence:

ATGATAATGG TGCGAAGACG ACAACGGCCC GCCAAGGAAG CCGCATCCTC ATCCTCTGGT 
GGAGCTTCCT CGGGATCGGG AATTCCTGTT GACCAGGCAC TGCCACTGAA TGTGGCGGGA 
AATCTGCTGG AGGATCAGTA TTTTGCCAGC CCCAAGCGGA AAGACTGCCG TTTGATGAAG 
GTCACTCAAA ATGGCCAACT GCCGGAAGCC ACAATGATGG CACACAACAA AGACAACAAA 
GCGGGTCGCA CAATAGGAGT TCCCTTGGCC ACGCGCTCAC AAACTCGCAC CATTGAGAAC 
TTCTTCAAAG CCAATGCTGC CGCTAAGGAT TCCCAAAAGA CAATACACAC AGAGGAGCAG 
TTGAATCTAG GAAATCAGGA ACTGAAACTG GACGATGAGG AGCTAAATGG ACAGATAAAA 
CTAGATGACG AGGTGCTAAA GCTGGCAGAC AAGCAGATAA ATGAAAACCT TCCCTTTGCC 
GACGAAGTAG ATGCCAAGGC AGAACAAAAG CTTATGGATG AGGAACTGCA GCAGGTGGTG 
GAGGAACTTC TGTTCGATGG CAGTTCCAGA GCGTCCTCCA ATTCGCCCTT TTATCAGCAT 
GATATGGATG TCATGCAGGA GATCCAGCAG ACTCCAGAAA TTCCACACAT CAAAAAGGTC 
ACAGAGCCGC TCGAGGGACT GGGCTCTCTG GCCGACTTCC AGACACATCG CTCTGCGCTG 
AGGGACAGCC ACAGTTCCAC GCACAGCAGC AGCACCGACA ACATCTTCCT GCAGGAGCCG 
GTACTGACCC TGGACATTGA TCGCACGCCC ACCAAAGCCT CCAGCATAAA GATCAATCGG 
AGCTTCGAAC TGGCCGGAGC CGTATTCTCA TCACCTCCGT CGGTGCTAAA CGCCTGTCTC 
AATGGACGCT TCAATCAAAT AGTAAGCCTG AATGGACAAA AGGAGGCGCT GGACTTGCCG 
CACTTCGATT TGGATCAACA TGACAGTAGT TCTTGCGACA GCGGAGTAGC CTGTGGTCTC 
ACTGCCAATA CAGAATCGCC AGCGGGGCAA CCCCGACGCA GAAAACCAGC CACACCGCAT 
CGCATCCTCT GCCCCTCGCC AATCAAAACG GCTTTGAAGG TAACTGGAGG GATTTGCAAG 
GTCGGATCTG CGGATCCATT GTCGCCGCGC AAGTCTCCTC GAAAACTGCC GACCACAACA 
GCAGCGGTGG CAGCCTGCAA GTCGCGTCGA AGACTGAACC AGCCAAAGCC ACAAGCGCCT 
TACCAGCCAC AGCTACAGAA ACCACCGTCA CAACAGCAGC AGCAGCAGCA GGATGATATC 
GTCGTGGTGC TAGACGACGA CGATGATGAA GGAGATGACG AGGACGACGT TCGAGCCCTG 
ATCAAGGCTG CCGAGGAGCG TGAAAACCAG AACAAAGCAC CAGCCACAGC GAACAGCAAC 
AAAGCAGGCA TGAAAACGAT GCTCAAGCCT GCGCCCGTGA AATCAAAGAC CAAAAGCAAG 
GGGCCAACGA AGGGACAACC GCCGCTGCCC TTGGCTGCCA CCAACGGCAA TCGTGAGATG 
ACGGACTTCT TCCCGGTGCG AAGGAGTGTG CGCAAGACTA AGACGGCCGT CAAGGAGGAA 
TGGATGCGAG GCTTGGAGCA AGCAGTGCTG GAAGAGCGCT GCGATGGTCT CCAAGTACGC 
CACTTTATGG GCAAGGGAAG AGGCGTAGTC GCCGATCGGC CCTTTAAACG CAACGAATTT 
GTGGTTGAGT ATGTGGGAGA TCTCATATCT ATAGGCGAAG CCGCGGAACG GGAGAAACGC 
TATGCGTTGG ACGAGAATGC TGGGTGCTAT ATGTACTACT TCAAGCACAA GTCCCAGCAG 
TACTGCATCG ATGCAACCGT TGACACCGGC AAGCTGGGGC GCCTCATCAA TCACTCGCGG 
GCTGGTAACC TAATGACCAA AGTAGTACTT ATCAAACAGC GGCCGCATCT CGTACTCCTG 
GCCAAGGACG ACATCGAGCC GGGCGAGGAA CTGACCTATG ACTACGGGGA CCGATCGAAG 
GAGTCACTAT TGCACCATCC TTGGTTGGCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PR-Set7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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