ITSN2 gene cDNA ORF clone, Felis catus(domestic cat)

mRNA	Protein	Name
XM_019827832.1	XP_019683391.1	intersectin-2 isoform X2
XM_019827833.1	XP_019683392.1	intersectin-2 isoform X3
XM_019827836.1	XP_019683395.1	intersectin-2 isoform X6
XM_019827838.1	XP_019683397.1	intersectin-2 isoform X8
XM_019827839.1	XP_019683398.1	intersectin-2 isoform X9
XM_023252050.1	XP_023107818.1	intersectin-2 isoform X1
XM_023252055.1	XP_023107823.1	intersectin-2 isoform X4
XM_023252057.1	XP_023107825.1	intersectin-2 isoform X5
XM_019827831.1	XP_019683390.1	intersectin-2 isoform X1
XM_023252051.1	XP_023107819.1	intersectin-2 isoform X1
XM_023252052.1	XP_023107820.1	intersectin-2 isoform X1
XM_023252053.1	XP_023107821.1	intersectin-2 isoform X2
XM_023252056.1	XP_023107824.1	intersectin-2 isoform X5
XM_023252058.1	XP_023107826.1	intersectin-2 isoform X6
XM_019827838.2	XP_019683397.2	intersectin-2 isoform X7
XM_019827837.1	XP_019683396.1	intersectin-2 isoform X7
XM_019827831.2	XP_019683390.2	intersectin-2 isoform X1
XM_023252054.1	XP_023107822.1	intersectin-2 isoform X3
XM_019827834.1	XP_019683393.1	intersectin-2 isoform X4
XM_019827835.1	XP_019683394.1	intersectin-2 isoform X5
XM_023252049.1	XP_023107817.1	intersectin-2 isoform X1
XM_019827835.2	XP_019683394.2	intersectin-2 isoform X5
XM_019827837.2	XP_019683396.2	intersectin-2 isoform X8
XM_023252059.1	XP_023107827.1	intersectin-2 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb40753	XM_019827831.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb40754	XM_019827832.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb40755	XM_019827833.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb40756	XM_019827834.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb40757	XM_019827836.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb40758	XM_019827838.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73977	XM_023252052.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73977	XM_019827831.2 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73977	XM_023252049.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73977	XM_023252051.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73977	XM_023252050.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73978	XM_023252053.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73979	XM_023252054.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73980	XM_023252055.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73981	XM_023252056.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73981	XM_019827835.2 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73981	XM_023252057.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73982	XM_023252058.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73983	XM_019827838.2 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb73984	XM_019827837.2 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFb73985	XM_023252059.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFb32214	XM_019827837.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFb32215	XM_019827839.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFb32216	XM_019827835.1 Latest version!	Felis catus intersectin 2 (ITSN2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ITSN2 ( XM_019827831.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683390.1 Felis catus intersectin 2 (ITSN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb40753
Clone ID Related Accession (Same CDS sequence)	XM_019827831.1
Accession Version	XM_019827831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTTTGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683390.1
CDS	84..5243
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827831.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827831.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTTTGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827832.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683391.1 Felis catus intersectin 2 (ITSN2), transcript variant X2, mRNA.

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CloneID	OFb40754
Clone ID Related Accession (Same CDS sequence)	XM_019827832.1
Accession Version	XM_019827832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTGAGCC AGCTACTGCT 
TTGGTGAATT ATAGAGCATT GTACCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT 
AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCGTAG GAGAACCTGG TTGGCTTTAT 
GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCCATCA 
GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC AGTATCTGCT 
ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT 
CGTACTGTTT CCCCTGGATC TGTATCACCG ATTCATGGCC AGGGACAGGT TGTAGAAAAC 
CTAAAGGCGC AGGCCCTGTG TTCTTGGACT GCAAAGAAGG ATAACCACTT GAACTTTTCA 
AAACATGATA TTATCACTGT GTTGGAACAG CAAGAAAATT GGTGGTTTGG AGAGGTGCAT 
GGAGGAAGAG GGTGGTTTCC AAAATCTTAT GTCAAAATAC TCCCTGGGAG TGAAGTCAAG 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAC 
ACAGGTGGAG AGGAATATAT TGCACTTTAT CCATATTCAA GTGTAGAACC TGGAGATTTG 
ACTTTCACTG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAATG GTGGACAGGA 
AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA ATCTGGAACA TCAAACAAGA AACCTGAGAT TGCTCAAGTA 
ACTTCAGCAT ATGTGGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCCGG GCAGTTAATA 
CTAATCCTAA AGAAAAATTC AAGTGGGTGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAACTACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGTAGCA 
AATAACGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT 
GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT 
GTTAAGATGA CAACAGACTC CGATCCAAGT CAACAGTGGT GTGCTGATCT CCAAAGCCTG 
GACACCATGC AGCCAGTTGA GAGGAAGAGG CAGGGCTATA TTCACGAGCT GATTCAGACC 
GAAGAGCGGT ACGTGGACGA CCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGTG 
GAGTCGGGCT TTCTCAGTGA AGGAGAAATG GCCTTGATTT TTGTAAACTG GAAAGAACTC 
ATCATGTCTA ACACAAAGCT ACTCAAGGCC TTGCGGGTAC GGAAGAAGAC CGGGGGAGAG 
AAGATGCCCG TGCAGATGAT TGGGGACATC CTGGCAGCCG AGCTGTCCCA CATGCAGGCT 
TATATCCGGT TCTGTAGCTG TCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACCGAT 
GAAGACACGG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG ATGTAAAGGA 
ATGCCCCTTT CCAGCTTCCT GCTGAAGCCC ATGCAGAGAA TCACCCGCTA CCCTCTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AGTCATGTGG ACCATTCCTC CCTAAAGCTG 
GCTCTTGAGC GGGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA 
AATTCAGACC GACTCGAGTG GATCCAGGCA CACGTTCAGT GTGAAGGTCT TGCAGAGCAA 
CTTATTTTCA ACTCCCTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA 
CTATACAAGA CCAAGAGCAA CAAGGAACTA CACGGATTCC TCTTCAATGA CTTTCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCAGGTT CTGAGAAGCT TTTCAGCTCT 
AAGTCCAATG CCCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCCTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCTCA CATTGACCGG 
GTCTACACCC TCCGAACAGA CAACATTAAC GAGAGGACTG CTTGGGTCCA GAAGATCAAG 
GCGGCATCTG AGCAGTACAT TGACACGGAA AAGAAGAAAC GGGAGAAGGC TTATCAAGCT 
CGCTCTCAAA AGACTTCAGG CATTGGGCGT CTGATGGTGC ACGTCATCGA AGCTACAGAA 
TTAAAAGCCT GTAAACCGAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTATA CCACCAGAAC CCTCCAGGAC ACACTCAATC CCAAGTGGAA CTTTAACTGC 
CAGTTCTTTA TTAAGGACCT TTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGC ACTGAAGTTC CAGTGGCAAA AATTCGCACA 
GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTCCTGC TGCACGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683391.1
CDS	385..5538
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827832.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827832.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTGAGCC AGCTACTGCT 
TTGGTGAATT ATAGAGCATT GTACCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT 
AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCGTAG GAGAACCTGG TTGGCTTTAT 
GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCCATCA 
GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC AGTATCTGCT 
ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT 
CGTACTGTTT CCCCTGGATC TGTATCACCG ATTCATGGCC AGGGACAGGT TGTAGAAAAC 
CTAAAGGCGC AGGCCCTGTG TTCTTGGACT GCAAAGAAGG ATAACCACTT GAACTTTTCA 
AAACATGATA TTATCACTGT GTTGGAACAG CAAGAAAATT GGTGGTTTGG AGAGGTGCAT 
GGAGGAAGAG GGTGGTTTCC AAAATCTTAT GTCAAAATAC TCCCTGGGAG TGAAGTCAAG 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAC 
ACAGGTGGAG AGGAATATAT TGCACTTTAT CCATATTCAA GTGTAGAACC TGGAGATTTG 
ACTTTCACTG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAATG GTGGACAGGA 
AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA ATCTGGAACA TCAAACAAGA AACCTGAGAT TGCTCAAGTA 
ACTTCAGCAT ATGTGGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCCGG GCAGTTAATA 
CTAATCCTAA AGAAAAATTC AAGTGGGTGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAACTACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGTAGCA 
AATAACGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT 
GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT 
GTTAAGATGA CAACAGACTC CGATCCAAGT CAACAGTGGT GTGCTGATCT CCAAAGCCTG 
GACACCATGC AGCCAGTTGA GAGGAAGAGG CAGGGCTATA TTCACGAGCT GATTCAGACC 
GAAGAGCGGT ACGTGGACGA CCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGTG 
GAGTCGGGCT TTCTCAGTGA AGGAGAAATG GCCTTGATTT TTGTAAACTG GAAAGAACTC 
ATCATGTCTA ACACAAAGCT ACTCAAGGCC TTGCGGGTAC GGAAGAAGAC CGGGGGAGAG 
AAGATGCCCG TGCAGATGAT TGGGGACATC CTGGCAGCCG AGCTGTCCCA CATGCAGGCT 
TATATCCGGT TCTGTAGCTG TCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACCGAT 
GAAGACACGG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG ATGTAAAGGA 
ATGCCCCTTT CCAGCTTCCT GCTGAAGCCC ATGCAGAGAA TCACCCGCTA CCCTCTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AGTCATGTGG ACCATTCCTC CCTAAAGCTG 
GCTCTTGAGC GGGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA 
AATTCAGACC GACTCGAGTG GATCCAGGCA CACGTTCAGT GTGAAGGTCT TGCAGAGCAA 
CTTATTTTCA ACTCCCTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA 
CTATACAAGA CCAAGAGCAA CAAGGAACTA CACGGATTCC TCTTCAATGA CTTTCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCAGGTT CTGAGAAGCT TTTCAGCTCT 
AAGTCCAATG CCCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCCTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCTCA CATTGACCGG 
GTCTACACCC TCCGAACAGA CAACATTAAC GAGAGGACTG CTTGGGTCCA GAAGATCAAG 
GCGGCATCTG AGCAGTACAT TGACACGGAA AAGAAGAAAC GGGAGAAGGC TTATCAAGCT 
CGCTCTCAAA AGACTTCAGG CATTGGGCGT CTGATGGTGC ACGTCATCGA AGCTACAGAA 
TTAAAAGCCT GTAAACCGAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTATA CCACCAGAAC CCTCCAGGAC ACACTCAATC CCAAGTGGAA CTTTAACTGC 
CAGTTCTTTA TTAAGGACCT TTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGC ACTGAAGTTC CAGTGGCAAA AATTCGCACA 
GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTCCTGC TGCACGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827833.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683392.1 Felis catus intersectin 2 (ITSN2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OFb40755
Clone ID Related Accession (Same CDS sequence)	XM_019827833.1
Accession Version	XM_019827833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGGA ACTGAGAGAA 
AGCTACAATA CACAGCAGTT AGCCCTTGAA CAACTTTATA AGATCAAACG TGACAAGTTG 
AAGGAAATTG AAAGAAAAAG GTCAGAGCTA ATACAGAAGA AGAAACTAGA AGATGAGGCT 
GTAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAGAG AAAACCTTAG AAAAGAGGAA 
GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAACACAAG AAAAAGTTCA AGAAGAGGAA 
CGGAAGGCTG AGGAGAAACC ATGTAAGGGT GAGCCAGCTA CTGCTTTGGT GAATTATAGA 
GCATTGTACC GCTTTGAAGC CAGGAGCCAT GATGAGATGA GTTTTAATTC CGGGGATATA 
ATACAGGTTG ATGAAAAAAC CGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA 
AATTTTGGCT GGTTTCCTTG CAACTATGTA GAAAAAATGC CATCAGGTGA AAAATCTGTG 
TCTCCTAAGA AGGCCTTACT TCCCCCTACA GTATCAGTAT CTGCTACCTC AACTTCTTCT 
GAATCACTTT CTTCAAATCA ACCAGCATCA GTGACTGATT ATCAAAATGT ATCTTTTACA 
AACCTAACTG TTAATACATC ATGGCAGAAA AAATCAGCTT TTACTCGTAC TGTTTCCCCT 
GGATCTGTAT CACCGATTCA TGGCCAGGGA CAGGTTGTAG AAAACCTAAA GGCGCAGGCC 
CTGTGTTCTT GGACTGCAAA GAAGGATAAC CACTTGAACT TTTCAAAACA TGATATTATC 
ACTGTGTTGG AACAGCAAGA AAATTGGTGG TTTGGAGAGG TGCATGGAGG AAGAGGGTGG 
TTTCCAAAAT CTTATGTCAA AATACTCCCT GGGAGTGAAG TCAAGCGGGA AGAACCAGAA 
GCTTTGTATG CAGCTGTAAA CAAGAAACCT ACCTCTGCAG CCTACACAGG TGGAGAGGAA 
TATATTGCAC TTTATCCATA TTCAAGTGTA GAACCTGGAG ATTTGACTTT CACTGAAGGT 
GAAGAAATAT TGGTGACCCA GAAAGATGGA GAATGGTGGA CAGGAAGTAT TGGAGATAGA 
ACTGGAATTT TTCCATCAAA TTATGTCAAA CCAAAAGATC AAGAGAGTTT TGGGAGTGCT 
AGCAAATCTG GAACATCAAA CAAGAAACCT GAGATTGCTC AAGTAACTTC AGCATATGTG 
GCTTCTGGTT CTGAACAACT TAGCCTTGCA CCCGGGCAGT TAATACTAAT CCTAAAGAAA 
AATTCAAGTG GGTGGTGGCA AGGGGAATTA CAGGCCAGAG GAAAAAAGCG ACAGAAAGGA 
TGGTTTCCTG CCAGCCATGT TAAACTTTTG GGTCCAAGTA GTGAAAGAAC TACACCTGCC 
TTTCATCCTG TATGTCAGGT GATTGCTATG TATGACTATG TAGCAAATAA CGAGGATGAG 
CTGAATTTCT CCAAGGGACA ACTTATCAAT GTTTTGAACA AAGATGATCC TGACTGGTGG 
CAAGGAGAAA TCAACGGGGT GACTGGTCTC TTTCCTTCAA ACTATGTTAA GATGACAACA 
GACTCCGATC CAAGTCAACA GTGGTGTGCT GATCTCCAAA GCCTGGACAC CATGCAGCCA 
GTTGAGAGGA AGAGGCAGGG CTATATTCAC GAGCTGATTC AGACCGAAGA GCGGTACGTG 
GACGACCTTC AGCTCGTCGT GGAGGTTTTT CAGAAACGGA TGGTGGAGTC GGGCTTTCTC 
AGTGAAGGAG AAATGGCCTT GATTTTTGTA AACTGGAAAG AACTCATCAT GTCTAACACA 
AAGCTACTCA AGGCCTTGCG GGTACGGAAG AAGACCGGGG GAGAGAAGAT GCCCGTGCAG 
ATGATTGGGG ACATCCTGGC AGCCGAGCTG TCCCACATGC AGGCTTATAT CCGGTTCTGT 
AGCTGTCAGC TTAATGGAGC AGCTCTGTTA CAGCAGAAGA CCGATGAAGA CACGGATTTC 
AAGGAATTTT TAAAGAAGCT GGCATCTGAC CCTCGATGTA AAGGAATGCC CCTTTCCAGC 
TTCCTGCTGA AGCCCATGCA GAGAATCACC CGCTACCCTC TGCTCATCAG AAGTATTCTG 
GAGAACACCC TGGAGAGTCA TGTGGACCAT TCCTCCCTAA AGCTGGCTCT TGAGCGGGCA 
GAGGAGCTTT GTTCCCAAGT TAATGAAGGA GTTCGGGAAA AAGAAAATTC AGACCGACTC 
GAGTGGATCC AGGCACACGT TCAGTGTGAA GGTCTTGCAG AGCAACTTAT TTTCAACTCC 
CTCACCAACT GCCTGGGGCC CCGGAAGCTT CTGCACAGTG GGAAACTATA CAAGACCAAG 
AGCAACAAGG AACTACACGG ATTCCTCTTC AATGACTTTC TGCTTCTTAC CTACATGGTC 
AAGCAGTTTG CTGTTTCCTC AGGTTCTGAG AAGCTTTTCA GCTCTAAGTC CAATGCCCAA 
TTCAAAATGT ATAAAACGCC CATTTTCCTG AATGAAGTCC TGGTGAAACT GCCCACAGAC 
CCTTCCAGCG ATGAGCCTGT CTTCCACATT TCTCACATTG ACCGGGTCTA CACCCTCCGA 
ACAGACAACA TTAACGAGAG GACTGCTTGG GTCCAGAAGA TCAAGGCGGC ATCTGAGCAG 
TACATTGACA CGGAAAAGAA GAAACGGGAG AAGGCTTATC AAGCTCGCTC TCAAAAGACT 
TCAGGCATTG GGCGTCTGAT GGTGCACGTC ATCGAAGCTA CAGAATTAAA AGCCTGTAAA 
CCGAATGGAA AGAGCAATCC ATACTGTGAA ATCAGCATGG GCTCCCAGAG CTATACCACC 
AGAACCCTCC AGGACACACT CAATCCCAAG TGGAACTTTA ACTGCCAGTT CTTTATTAAG 
GACCTTTACC AAGATGTGCT GTGTCTCACC ATGTTTGACA GAGACCAGTT TTCACCAGAT 
GATTTCCTGG GTCGCACTGA AGTTCCAGTG GCAAAAATTC GCACAGAACA GGAAAGCAAA 
GGCCCCACAA CCCGCCGACT CCTGCTGCAC GAGGTCCCCA CCGGGGAGGT CTGGGTCCGC 
TTTGACCTGC AGCTTTTTGA GCAAAAAACT CTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683392.1
CDS	380..5458
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827833.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827833.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGGA ACTGAGAGAA 
AGCTACAATA CACAGCAGTT AGCCCTTGAA CAACTTTATA AGATCAAACG TGACAAGTTG 
AAGGAAATTG AAAGAAAAAG GTCAGAGCTA ATACAGAAGA AGAAACTAGA AGATGAGGCT 
GTAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAGAG AAAACCTTAG AAAAGAGGAA 
GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAACACAAG AAAAAGTTCA AGAAGAGGAA 
CGGAAGGCTG AGGAGAAACC ATGTAAGGGT GAGCCAGCTA CTGCTTTGGT GAATTATAGA 
GCATTGTACC GCTTTGAAGC CAGGAGCCAT GATGAGATGA GTTTTAATTC CGGGGATATA 
ATACAGGTTG ATGAAAAAAC CGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA 
AATTTTGGCT GGTTTCCTTG CAACTATGTA GAAAAAATGC CATCAGGTGA AAAATCTGTG 
TCTCCTAAGA AGGCCTTACT TCCCCCTACA GTATCAGTAT CTGCTACCTC AACTTCTTCT 
GAATCACTTT CTTCAAATCA ACCAGCATCA GTGACTGATT ATCAAAATGT ATCTTTTACA 
AACCTAACTG TTAATACATC ATGGCAGAAA AAATCAGCTT TTACTCGTAC TGTTTCCCCT 
GGATCTGTAT CACCGATTCA TGGCCAGGGA CAGGTTGTAG AAAACCTAAA GGCGCAGGCC 
CTGTGTTCTT GGACTGCAAA GAAGGATAAC CACTTGAACT TTTCAAAACA TGATATTATC 
ACTGTGTTGG AACAGCAAGA AAATTGGTGG TTTGGAGAGG TGCATGGAGG AAGAGGGTGG 
TTTCCAAAAT CTTATGTCAA AATACTCCCT GGGAGTGAAG TCAAGCGGGA AGAACCAGAA 
GCTTTGTATG CAGCTGTAAA CAAGAAACCT ACCTCTGCAG CCTACACAGG TGGAGAGGAA 
TATATTGCAC TTTATCCATA TTCAAGTGTA GAACCTGGAG ATTTGACTTT CACTGAAGGT 
GAAGAAATAT TGGTGACCCA GAAAGATGGA GAATGGTGGA CAGGAAGTAT TGGAGATAGA 
ACTGGAATTT TTCCATCAAA TTATGTCAAA CCAAAAGATC AAGAGAGTTT TGGGAGTGCT 
AGCAAATCTG GAACATCAAA CAAGAAACCT GAGATTGCTC AAGTAACTTC AGCATATGTG 
GCTTCTGGTT CTGAACAACT TAGCCTTGCA CCCGGGCAGT TAATACTAAT CCTAAAGAAA 
AATTCAAGTG GGTGGTGGCA AGGGGAATTA CAGGCCAGAG GAAAAAAGCG ACAGAAAGGA 
TGGTTTCCTG CCAGCCATGT TAAACTTTTG GGTCCAAGTA GTGAAAGAAC TACACCTGCC 
TTTCATCCTG TATGTCAGGT GATTGCTATG TATGACTATG TAGCAAATAA CGAGGATGAG 
CTGAATTTCT CCAAGGGACA ACTTATCAAT GTTTTGAACA AAGATGATCC TGACTGGTGG 
CAAGGAGAAA TCAACGGGGT GACTGGTCTC TTTCCTTCAA ACTATGTTAA GATGACAACA 
GACTCCGATC CAAGTCAACA GTGGTGTGCT GATCTCCAAA GCCTGGACAC CATGCAGCCA 
GTTGAGAGGA AGAGGCAGGG CTATATTCAC GAGCTGATTC AGACCGAAGA GCGGTACGTG 
GACGACCTTC AGCTCGTCGT GGAGGTTTTT CAGAAACGGA TGGTGGAGTC GGGCTTTCTC 
AGTGAAGGAG AAATGGCCTT GATTTTTGTA AACTGGAAAG AACTCATCAT GTCTAACACA 
AAGCTACTCA AGGCCTTGCG GGTACGGAAG AAGACCGGGG GAGAGAAGAT GCCCGTGCAG 
ATGATTGGGG ACATCCTGGC AGCCGAGCTG TCCCACATGC AGGCTTATAT CCGGTTCTGT 
AGCTGTCAGC TTAATGGAGC AGCTCTGTTA CAGCAGAAGA CCGATGAAGA CACGGATTTC 
AAGGAATTTT TAAAGAAGCT GGCATCTGAC CCTCGATGTA AAGGAATGCC CCTTTCCAGC 
TTCCTGCTGA AGCCCATGCA GAGAATCACC CGCTACCCTC TGCTCATCAG AAGTATTCTG 
GAGAACACCC TGGAGAGTCA TGTGGACCAT TCCTCCCTAA AGCTGGCTCT TGAGCGGGCA 
GAGGAGCTTT GTTCCCAAGT TAATGAAGGA GTTCGGGAAA AAGAAAATTC AGACCGACTC 
GAGTGGATCC AGGCACACGT TCAGTGTGAA GGTCTTGCAG AGCAACTTAT TTTCAACTCC 
CTCACCAACT GCCTGGGGCC CCGGAAGCTT CTGCACAGTG GGAAACTATA CAAGACCAAG 
AGCAACAAGG AACTACACGG ATTCCTCTTC AATGACTTTC TGCTTCTTAC CTACATGGTC 
AAGCAGTTTG CTGTTTCCTC AGGTTCTGAG AAGCTTTTCA GCTCTAAGTC CAATGCCCAA 
TTCAAAATGT ATAAAACGCC CATTTTCCTG AATGAAGTCC TGGTGAAACT GCCCACAGAC 
CCTTCCAGCG ATGAGCCTGT CTTCCACATT TCTCACATTG ACCGGGTCTA CACCCTCCGA 
ACAGACAACA TTAACGAGAG GACTGCTTGG GTCCAGAAGA TCAAGGCGGC ATCTGAGCAG 
TACATTGACA CGGAAAAGAA GAAACGGGAG AAGGCTTATC AAGCTCGCTC TCAAAAGACT 
TCAGGCATTG GGCGTCTGAT GGTGCACGTC ATCGAAGCTA CAGAATTAAA AGCCTGTAAA 
CCGAATGGAA AGAGCAATCC ATACTGTGAA ATCAGCATGG GCTCCCAGAG CTATACCACC 
AGAACCCTCC AGGACACACT CAATCCCAAG TGGAACTTTA ACTGCCAGTT CTTTATTAAG 
GACCTTTACC AAGATGTGCT GTGTCTCACC ATGTTTGACA GAGACCAGTT TTCACCAGAT 
GATTTCCTGG GTCGCACTGA AGTTCCAGTG GCAAAAATTC GCACAGAACA GGAAAGCAAA 
GGCCCCACAA CCCGCCGACT CCTGCTGCAC GAGGTCCCCA CCGGGGAGGT CTGGGTCCGC 
TTTGACCTGC AGCTTTTTGA GCAAAAAACT CTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827834.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683393.1 Felis catus intersectin 2 (ITSN2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OFb40756
Clone ID Related Accession (Same CDS sequence)	XM_019827834.1
Accession Version	XM_019827834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTTTGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGATTC TGGAGAACAC CCTGGAGAGT 
CATGTGGACC ATTCCTCCCT AAAGCTGGCT CTTGAGCGGG CAGAGGAGCT TTGTTCCCAA 
GTTAATGAAG GAGTTCGGGA AAAAGAAAAT TCAGACCGAC TCGAGTGGAT CCAGGCACAC 
GTTCAGTGTG AAGGTCTTGC AGAGCAACTT ATTTTCAACT CCCTCACCAA CTGCCTGGGG 
CCCCGGAAGC TTCTGCACAG TGGGAAACTA TACAAGACCA AGAGCAACAA GGAACTACAC 
GGATTCCTCT TCAATGACTT TCTGCTTCTT ACCTACATGG TCAAGCAGTT TGCTGTTTCC 
TCAGGTTCTG AGAAGCTTTT CAGCTCTAAG TCCAATGCCC AATTCAAAAT GTATAAAACG 
CCCATTTTCC TGAATGAAGT CCTGGTGAAA CTGCCCACAG ACCCTTCCAG CGATGAGCCT 
GTCTTCCACA TTTCTCACAT TGACCGGGTC TACACCCTCC GAACAGACAA CATTAACGAG 
AGGACTGCTT GGGTCCAGAA GATCAAGGCG GCATCTGAGC AGTACATTGA CACGGAAAAG 
AAGAAACGGG AGAAGGCTTA TCAAGCTCGC TCTCAAAAGA CTTCAGGCAT TGGGCGTCTG 
ATGGTGCACG TCATCGAAGC TACAGAATTA AAAGCCTGTA AACCGAATGG AAAGAGCAAT 
CCATACTGTG AAATCAGCAT GGGCTCCCAG AGCTATACCA CCAGAACCCT CCAGGACACA 
CTCAATCCCA AGTGGAACTT TAACTGCCAG TTCTTTATTA AGGACCTTTA CCAAGATGTG 
CTGTGTCTCA CCATGTTTGA CAGAGACCAG TTTTCACCAG ATGATTTCCT GGGTCGCACT 
GAAGTTCCAG TGGCAAAAAT TCGCACAGAA CAGGAAAGCA AAGGCCCCAC AACCCGCCGA 
CTCCTGCTGC ACGAGGTCCC CACCGGGGAG GTCTGGGTCC GCTTTGACCT GCAGCTTTTT 
GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683393.1
CDS	384..5444
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827834.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827834.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTTTGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGATTC TGGAGAACAC CCTGGAGAGT 
CATGTGGACC ATTCCTCCCT AAAGCTGGCT CTTGAGCGGG CAGAGGAGCT TTGTTCCCAA 
GTTAATGAAG GAGTTCGGGA AAAAGAAAAT TCAGACCGAC TCGAGTGGAT CCAGGCACAC 
GTTCAGTGTG AAGGTCTTGC AGAGCAACTT ATTTTCAACT CCCTCACCAA CTGCCTGGGG 
CCCCGGAAGC TTCTGCACAG TGGGAAACTA TACAAGACCA AGAGCAACAA GGAACTACAC 
GGATTCCTCT TCAATGACTT TCTGCTTCTT ACCTACATGG TCAAGCAGTT TGCTGTTTCC 
TCAGGTTCTG AGAAGCTTTT CAGCTCTAAG TCCAATGCCC AATTCAAAAT GTATAAAACG 
CCCATTTTCC TGAATGAAGT CCTGGTGAAA CTGCCCACAG ACCCTTCCAG CGATGAGCCT 
GTCTTCCACA TTTCTCACAT TGACCGGGTC TACACCCTCC GAACAGACAA CATTAACGAG 
AGGACTGCTT GGGTCCAGAA GATCAAGGCG GCATCTGAGC AGTACATTGA CACGGAAAAG 
AAGAAACGGG AGAAGGCTTA TCAAGCTCGC TCTCAAAAGA CTTCAGGCAT TGGGCGTCTG 
ATGGTGCACG TCATCGAAGC TACAGAATTA AAAGCCTGTA AACCGAATGG AAAGAGCAAT 
CCATACTGTG AAATCAGCAT GGGCTCCCAG AGCTATACCA CCAGAACCCT CCAGGACACA 
CTCAATCCCA AGTGGAACTT TAACTGCCAG TTCTTTATTA AGGACCTTTA CCAAGATGTG 
CTGTGTCTCA CCATGTTTGA CAGAGACCAG TTTTCACCAG ATGATTTCCT GGGTCGCACT 
GAAGTTCCAG TGGCAAAAAT TCGCACAGAA CAGGAAAGCA AAGGCCCCAC AACCCGCCGA 
CTCCTGCTGC ACGAGGTCCC CACCGGGGAG GTCTGGGTCC GCTTTGACCT GCAGCTTTTT 
GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827836.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683395.1 Felis catus intersectin 2 (ITSN2), transcript variant X6, mRNA.

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CloneID	OFb40757
Clone ID Related Accession (Same CDS sequence)	XM_019827836.1
Accession Version	XM_019827836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTGAGCCAG CTACTGCTTT GGTGAATTAT AGAGCATTGT ACCGCTTTGA AGCCAGGAGC 
CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCGTAGGA 
GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT 
GTAGAAAAAA TGCCATCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT 
ACAGTATCAG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG 
AAAAAATCAG CTTTTACTCG TACTGTTTCC CCTGGATCTG TATCACCGAT TCATGGCCAG 
GGACAGGTTG TAGAAAACCT AAAGGCGCAG GCCCTGTGTT CTTGGACTGC AAAGAAGGAT 
AACCACTTGA ACTTTTCAAA ACATGATATT ATCACTGTGT TGGAACAGCA AGAAAATTGG 
TGGTTTGGAG AGGTGCATGG AGGAAGAGGG TGGTTTCCAA AATCTTATGT CAAAATACTC 
CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAACAAGAAA 
CCTACCTCTG CAGCCTACAC AGGTGGAGAG GAATATATTG CACTTTATCC ATATTCAAGT 
GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAATGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC 
AAACCAAAAG ATCAAGAGAG TTTTGGGAGT GCTAGCAAAT CTGGAACATC AAACAAGAAA 
CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTGGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCCGGGC AGTTAATACT AATCCTAAAG AAAAATTCAA GTGGGTGGTG GCAAGGGGAA 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AACTACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGTAGCAAA TAACGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC 
AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCCG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTCC AAAGCCTGGA CACCATGCAG CCAGTTGAGA GGAAGAGGCA GGGCTATATT 
CACGAGCTGA TTCAGACCGA AGAGCGGTAC GTGGACGACC TTCAGCTCGT CGTGGAGGTT 
TTTCAGAAAC GGATGGTGGA GTCGGGCTTT CTCAGTGAAG GAGAAATGGC CTTGATTTTT 
GTAAACTGGA AAGAACTCAT CATGTCTAAC ACAAAGCTAC TCAAGGCCTT GCGGGTACGG 
AAGAAGACCG GGGGAGAGAA GATGCCCGTG CAGATGATTG GGGACATCCT GGCAGCCGAG 
CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACCGATGA AGACACGGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT 
GACCCTCGAT GTAAAGGAAT GCCCCTTTCC AGCTTCCTGC TGAAGCCCAT GCAGAGAATC 
ACCCGCTACC CTCTGCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAG TCATGTGGAC 
CATTCCTCCC TAAAGCTGGC TCTTGAGCGG GCAGAGGAGC TTTGTTCCCA AGTTAATGAA 
GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTCGAGTGGA TCCAGGCACA CGTTCAGTGT 
GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCCCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTTCTGCACA GTGGGAAACT ATACAAGACC AAGAGCAACA AGGAACTACA CGGATTCCTC 
TTCAATGACT TTCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCAGGTTCT 
GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCCTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCTCACA TTGACCGGGT CTACACCCTC CGAACAGACA ACATTAACGA GAGGACTGCT 
TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATTG ACACGGAAAA GAAGAAACGG 
GAGAAGGCTT ATCAAGCTCG CTCTCAAAAG ACTTCAGGCA TTGGGCGTCT GATGGTGCAC 
GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCGAATG GAAAGAGCAA TCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCAGGACAC ACTCAATCCC 
AAGTGGAACT TTAACTGCCA GTTCTTTATT AAGGACCTTT ACCAAGATGT GCTGTGTCTC 
ACCATGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGCAC TGAAGTTCCA 
GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTCCTGCTG 
CACGAGGTCC CCACCGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683395.1
CDS	384..5435
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827836.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827836.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTGAGCCAG CTACTGCTTT GGTGAATTAT AGAGCATTGT ACCGCTTTGA AGCCAGGAGC 
CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCGTAGGA 
GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT 
GTAGAAAAAA TGCCATCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT 
ACAGTATCAG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG 
AAAAAATCAG CTTTTACTCG TACTGTTTCC CCTGGATCTG TATCACCGAT TCATGGCCAG 
GGACAGGTTG TAGAAAACCT AAAGGCGCAG GCCCTGTGTT CTTGGACTGC AAAGAAGGAT 
AACCACTTGA ACTTTTCAAA ACATGATATT ATCACTGTGT TGGAACAGCA AGAAAATTGG 
TGGTTTGGAG AGGTGCATGG AGGAAGAGGG TGGTTTCCAA AATCTTATGT CAAAATACTC 
CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAACAAGAAA 
CCTACCTCTG CAGCCTACAC AGGTGGAGAG GAATATATTG CACTTTATCC ATATTCAAGT 
GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAATGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC 
AAACCAAAAG ATCAAGAGAG TTTTGGGAGT GCTAGCAAAT CTGGAACATC AAACAAGAAA 
CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTGGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCCGGGC AGTTAATACT AATCCTAAAG AAAAATTCAA GTGGGTGGTG GCAAGGGGAA 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AACTACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGTAGCAAA TAACGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC 
AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCCG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTCC AAAGCCTGGA CACCATGCAG CCAGTTGAGA GGAAGAGGCA GGGCTATATT 
CACGAGCTGA TTCAGACCGA AGAGCGGTAC GTGGACGACC TTCAGCTCGT CGTGGAGGTT 
TTTCAGAAAC GGATGGTGGA GTCGGGCTTT CTCAGTGAAG GAGAAATGGC CTTGATTTTT 
GTAAACTGGA AAGAACTCAT CATGTCTAAC ACAAAGCTAC TCAAGGCCTT GCGGGTACGG 
AAGAAGACCG GGGGAGAGAA GATGCCCGTG CAGATGATTG GGGACATCCT GGCAGCCGAG 
CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACCGATGA AGACACGGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT 
GACCCTCGAT GTAAAGGAAT GCCCCTTTCC AGCTTCCTGC TGAAGCCCAT GCAGAGAATC 
ACCCGCTACC CTCTGCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAG TCATGTGGAC 
CATTCCTCCC TAAAGCTGGC TCTTGAGCGG GCAGAGGAGC TTTGTTCCCA AGTTAATGAA 
GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTCGAGTGGA TCCAGGCACA CGTTCAGTGT 
GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCCCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTTCTGCACA GTGGGAAACT ATACAAGACC AAGAGCAACA AGGAACTACA CGGATTCCTC 
TTCAATGACT TTCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCAGGTTCT 
GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCCTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCTCACA TTGACCGGGT CTACACCCTC CGAACAGACA ACATTAACGA GAGGACTGCT 
TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATTG ACACGGAAAA GAAGAAACGG 
GAGAAGGCTT ATCAAGCTCG CTCTCAAAAG ACTTCAGGCA TTGGGCGTCT GATGGTGCAC 
GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCGAATG GAAAGAGCAA TCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCAGGACAC ACTCAATCCC 
AAGTGGAACT TTAACTGCCA GTTCTTTATT AAGGACCTTT ACCAAGATGT GCTGTGTCTC 
ACCATGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGCAC TGAAGTTCCA 
GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTCCTGCTG 
CACGAGGTCC CCACCGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827838.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683397.1 Felis catus intersectin 2 (ITSN2), transcript variant X8, mRNA.

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CloneID	OFb40758
Clone ID Related Accession (Same CDS sequence)	XM_019827838.1
Accession Version	XM_019827838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTA TTACCTCTGA AGAACGGACT AAGCATGACA AGCAGTTTGA TAACCTCAAA 
CCCTCAGGAG GTTACATAAC AGGTGATCAA GCACGTACCT TTTTCTTACA GTCAGGCCTG 
CCGGCTCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG 
GATCAGCAAG AATTCTCCAT AGCGATGAAG CTCATCAAAC TGAAGCTACA AGGCCAGCAG 
CTGCCCGTGG TCCTCCCTCC TATTATGAAA CAACCTCCTA TGTTCTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCACTCATT GCCTCCAGTT 
GCACCTATGG CAACCCCCTT ACCCTCTGCG ACTTCAGGGA CCACCCTTCC TCCCTTAATG 
ATGCCTGCTC CCCTAGTGCC TTCTGTTAGC ACATCGTCAC TACCAAATGG AACCGCTGGC 
CTCATTCAGC CGTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC 
AGTCTCATGA TGGGAGGACT CGGCGGTGCT AGTTTACAGA AAGCCCAGTC TCTGATTGAT 
TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCACTCT CTGGAAACTC GCCCAAGACT 
GGGACCTTAG AGTGGGCGGT TCCTCAGCCT TCAAGGTTAA AATATCGGCA AAAATTTAAT 
AGTCTTGACA AAAGCATGAG TGGATATCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT 
CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATTTGGA CCCTTGCTGA CATTGATGGC 
GACGGACAGC TGAAAGCTGA AGAGTTCATC CTTGCGATGC ATCTCACTGA CATGGCCAAA 
GCTGGACAGC CCTTGCCACT GACGTTGCCT CCTGAACTTG TCCCTCCATC TTTCAGAGGA 
GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCCT ATCAGAAAAT TCAAGAAGAG 
GAACCTCAGA AAAAATTACC AGTTACTTTC GAAGACAAGC GGAAGGCCAA CTACGAGCGA 
GGGAACGTGG AACTGGAGAA GCGGCGCCAA GCCCTGATGG AGCAGCAGCA GAGGGAGGCA 
GAGCGAAAAG CCCAGAAAGA AAAGGAAGAG TGGGAGCGAA AACAGAGAGA ATTACAGGAA 
CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCTTGG AGAAGCAGAG GGAATTGGAG 
AGACAGCGGG AGGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAA 
CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGGATTCGTC GCCAGGAGCT TCTCAATCAA 
AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG TCTTCATCTC 
GAGTTGGAAG CACTGAATGG CAAACACCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA 
CTCCGAAAAC AAACTCAAAA GACTGAGCTG GAAGTTCTAG ATAAACAGTG TGACCTGGAA 
ATTATGGAAA TCAAGCAACT CCAACAAGAA CTTCAGGATT ATCAAAATAA ACTTATCTAT 
TTGGTACCGG AGAAGCAATT ATTAAATGAA AGAATTAAGA ACATGCAGCT TGGTAACACA 
CCTGATTTAG GGATTGGTTT GCTTCATAAA AAATCATTGG AAAAGGAAGA ATTATGCCAA 
AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT ATCTGAAATG 
GATTCATTTA ACAATCAATT AAAGTGTGGG AATATGGATG ACTCTGTTCT GCAGTGCCTT 
TTGTCTCTGC TAAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT GAGAGAAAGC 
TACAATACAC AGCAGTTAGC CCTTGAACAA CTTTATAAGA TCAAACGTGA CAAGTTGAAG 
GAAATTGAAA GAAAAAGGTC AGAGCTAATA CAGAAGAAGA AACTAGAAGA TGAGGCTGTA 
AGGAAAGCAA AGCAAGGAAA AGAAAACTTA TGGAGAGAAA ACCTTAGAAA AGAGGAAGAA 
GAAAAACAAA AGCGACTCCA GGAAGAAAAA ACACAAGAAA AAGTTCAAGA AGAGGAACGG 
AAGGCTGAGG AGAAACCATG TAAGGGTGAG CCAGCTACTG CTTTGGTGAA TTATAGAGCA 
TTGTACCGCT TTGAAGCCAG GAGCCATGAT GAGATGAGTT TTAATTCCGG GGATATAATA 
CAGGTTGATG AAAAAACCGT AGGAGAACCT GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT 
TTTGGCTGGT TTCCTTGCAA CTATGTAGAA AAAATGCCAT CAGGTGAAAA ATCTGTGTCT 
CCTAAGAAGG CCTTACTTCC CCCTACAGTA TCAGTATCTG CTACCTCAAC TTCTTCTGAA 
TCACTTTCTT CAAATCAACC AGCATCAGTG ACTGATTATC AAAATGTATC TTTTACAAAC 
CTAACTGTTA ATACATCATG GCAGAAAAAA TCAGCTTTTA CTCGTACTGT TTCCCCTGGA 
TCTGTATCAC CGATTCATGG CCAGGGACAG GTTGTAGAAA ACCTAAAGGC GCAGGCCCTG 
TGTTCTTGGA CTGCAAAGAA GGATAACCAC TTGAACTTTT CAAAACATGA TATTATCACT 
GTGTTGGAAC AGCAAGAAAA TTGGTGGTTT GGAGAGGTGC ATGGAGGAAG AGGGTGGTTT 
CCAAAATCTT ATGTCAAAAT ACTCCCTGGG AGTGAAGTCA AGCGGGAAGA ACCAGAAGCT 
TTGTATGCAG CTGTAAACAA GAAACCTACC TCTGCAGCCT ACACAGGTGG AGAGGAATAT 
ATTGCACTTT ATCCATATTC AAGTGTAGAA CCTGGAGATT TGACTTTCAC TGAAGGTGAA 
GAAATATTGG TGACCCAGAA AGATGGAGAA TGGTGGACAG GAAGTATTGG AGATAGAACT 
GGAATTTTTC CATCAAATTA TGTCAAACCA AAAGATCAAG AGAGTTTTGG GAGTGCTAGC 
AAATCTGGAA CATCAAACAA GAAACCTGAG ATTGCTCAAG TAACTTCAGC ATATGTGGCT 
TCTGGTTCTG AACAACTTAG CCTTGCACCC GGGCAGTTAA TACTAATCCT AAAGAAAAAT 
TCAAGTGGGT GGTGGCAAGG GGAATTACAG GCCAGAGGAA AAAAGCGACA GAAAGGATGG 
TTTCCTGCCA GCCATGTTAA ACTTTTGGGT CCAAGTAGTG AAAGAACTAC ACCTGCCTTT 
CATCCTGTAT GTCAGGTGAT TGCTATGTAT GACTATGTAG CAAATAACGA GGATGAGCTG 
AATTTCTCCA AGGGACAACT TATCAATGTT TTGAACAAAG ATGATCCTGA CTGGTGGCAA 
GGAGAAATCA ACGGGGTGAC TGGTCTCTTT CCTTCAAACT ATGTTAAGAT GACAACAGAC 
TCCGATCCAA GTCAACAGTG GTGTGCTGAT CTCCAAAGCC TGGACACCAT GCAGCCAGTT 
GAGAGGAAGA GGCAGGGCTA TATTCACGAG CTGATTCAGA CCGAAGAGCG GTACGTGGAC 
GACCTTCAGC TCGTCGTGGA GGTTTTTCAG AAACGGATGG TGGAGTCGGG CTTTCTCAGT 
GAAGGAGAAA TGGCCTTGAT TTTTGTAAAC TGGAAAGAAC TCATCATGTC TAACACAAAG 
CTACTCAAGG CCTTGCGGGT ACGGAAGAAG ACCGGGGGAG AGAAGATGCC CGTGCAGATG 
ATTGGGGACA TCCTGGCAGC CGAGCTGTCC CACATGCAGG CTTATATCCG GTTCTGTAGC 
TGTCAGCTTA ATGGAGCAGC TCTGTTACAG CAGAAGACCG ATGAAGACAC GGATTTCAAG 
GAATTTTTAA AGAAGCTGGC ATCTGACCCT CGATGTAAAG GAATGCCCCT TTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG AATCACCCGC TACCCTCTGC TCATCAGAAG TATTCTGGAG 
AACACCCTGG AGAGTCATGT GGACCATTCC TCCCTAAAGC TGGCTCTTGA GCGGGCAGAG 
GAGCTTTGTT CCCAAGTTAA TGAAGGAGTT CGGGAAAAAG AAAATTCAGA CCGACTCGAG 
TGGATCCAGG CACACGTTCA GTGTGAAGGT CTTGCAGAGC AACTTATTTT CAACTCCCTC 
ACCAACTGCC TGGGGCCCCG GAAGCTTCTG CACAGTGGGA AACTATACAA GACCAAGAGC 
AACAAGGAAC TACACGGATT CCTCTTCAAT GACTTTCTGC TTCTTACCTA CATGGTCAAG 
CAGTTTGCTG TTTCCTCAGG TTCTGAGAAG CTTTTCAGCT CTAAGTCCAA TGCCCAATTC 
AAAATGTATA AAACGCCCAT TTTCCTGAAT GAAGTCCTGG TGAAACTGCC CACAGACCCT 
TCCAGCGATG AGCCTGTCTT CCACATTTCT CACATTGACC GGGTCTACAC CCTCCGAACA 
GACAACATTA ACGAGAGGAC TGCTTGGGTC CAGAAGATCA AGGCGGCATC TGAGCAGTAC 
ATTGACACGG AAAAGAAGAA ACGGGAGAAG GCTTATCAAG CTCGCTCTCA AAAGACTTCA 
GGCATTGGGC GTCTGATGGT GCACGTCATC GAAGCTACAG AATTAAAAGC CTGTAAACCG 
AATGGAAAGA GCAATCCATA CTGTGAAATC AGCATGGGCT CCCAGAGCTA TACCACCAGA 
ACCCTCCAGG ACACACTCAA TCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAC 
CTTTACCAAG ATGTGCTGTG TCTCACCATG TTTGACAGAG ACCAGTTTTC ACCAGATGAT 
TTCCTGGGTC GCACTGAAGT TCCAGTGGCA AAAATTCGCA CAGAACAGGA AAGCAAAGGC 
CCCACAACCC GCCGACTCCT GCTGCACGAG GTCCCCACCG GGGAGGTCTG GGTCCGCTTT 
GACCTGCAGC TTTTTGAGCA AAAAACTCTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683397.1
CDS	111..5126
Translation	MWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827838.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827838.1

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGGACT AAGCATGACA AGCAGTTTGA TAACCTCAAA 
CCCTCAGGAG GTTACATAAC AGGTGATCAA GCACGTACCT TTTTCTTACA GTCAGGCCTG 
CCGGCTCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG 
GATCAGCAAG AATTCTCCAT AGCGATGAAG CTCATCAAAC TGAAGCTACA AGGCCAGCAG 
CTGCCCGTGG TCCTCCCTCC TATTATGAAA CAACCTCCTA TGTTCTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCACTCATT GCCTCCAGTT 
GCACCTATGG CAACCCCCTT ACCCTCTGCG ACTTCAGGGA CCACCCTTCC TCCCTTAATG 
ATGCCTGCTC CCCTAGTGCC TTCTGTTAGC ACATCGTCAC TACCAAATGG AACCGCTGGC 
CTCATTCAGC CGTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC 
AGTCTCATGA TGGGAGGACT CGGCGGTGCT AGTTTACAGA AAGCCCAGTC TCTGATTGAT 
TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCACTCT CTGGAAACTC GCCCAAGACT 
GGGACCTTAG AGTGGGCGGT TCCTCAGCCT TCAAGGTTAA AATATCGGCA AAAATTTAAT 
AGTCTTGACA AAAGCATGAG TGGATATCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT 
CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATTTGGA CCCTTGCTGA CATTGATGGC 
GACGGACAGC TGAAAGCTGA AGAGTTCATC CTTGCGATGC ATCTCACTGA CATGGCCAAA 
GCTGGACAGC CCTTGCCACT GACGTTGCCT CCTGAACTTG TCCCTCCATC TTTCAGAGGA 
GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCCT ATCAGAAAAT TCAAGAAGAG 
GAACCTCAGA AAAAATTACC AGTTACTTTC GAAGACAAGC GGAAGGCCAA CTACGAGCGA 
GGGAACGTGG AACTGGAGAA GCGGCGCCAA GCCCTGATGG AGCAGCAGCA GAGGGAGGCA 
GAGCGAAAAG CCCAGAAAGA AAAGGAAGAG TGGGAGCGAA AACAGAGAGA ATTACAGGAA 
CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCTTGG AGAAGCAGAG GGAATTGGAG 
AGACAGCGGG AGGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAA 
CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGGATTCGTC GCCAGGAGCT TCTCAATCAA 
AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG TCTTCATCTC 
GAGTTGGAAG CACTGAATGG CAAACACCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA 
CTCCGAAAAC AAACTCAAAA GACTGAGCTG GAAGTTCTAG ATAAACAGTG TGACCTGGAA 
ATTATGGAAA TCAAGCAACT CCAACAAGAA CTTCAGGATT ATCAAAATAA ACTTATCTAT 
TTGGTACCGG AGAAGCAATT ATTAAATGAA AGAATTAAGA ACATGCAGCT TGGTAACACA 
CCTGATTTAG GGATTGGTTT GCTTCATAAA AAATCATTGG AAAAGGAAGA ATTATGCCAA 
AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT ATCTGAAATG 
GATTCATTTA ACAATCAATT AAAGTGTGGG AATATGGATG ACTCTGTTCT GCAGTGCCTT 
TTGTCTCTGC TAAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT GAGAGAAAGC 
TACAATACAC AGCAGTTAGC CCTTGAACAA CTTTATAAGA TCAAACGTGA CAAGTTGAAG 
GAAATTGAAA GAAAAAGGTC AGAGCTAATA CAGAAGAAGA AACTAGAAGA TGAGGCTGTA 
AGGAAAGCAA AGCAAGGAAA AGAAAACTTA TGGAGAGAAA ACCTTAGAAA AGAGGAAGAA 
GAAAAACAAA AGCGACTCCA GGAAGAAAAA ACACAAGAAA AAGTTCAAGA AGAGGAACGG 
AAGGCTGAGG AGAAACCATG TAAGGGTGAG CCAGCTACTG CTTTGGTGAA TTATAGAGCA 
TTGTACCGCT TTGAAGCCAG GAGCCATGAT GAGATGAGTT TTAATTCCGG GGATATAATA 
CAGGTTGATG AAAAAACCGT AGGAGAACCT GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT 
TTTGGCTGGT TTCCTTGCAA CTATGTAGAA AAAATGCCAT CAGGTGAAAA ATCTGTGTCT 
CCTAAGAAGG CCTTACTTCC CCCTACAGTA TCAGTATCTG CTACCTCAAC TTCTTCTGAA 
TCACTTTCTT CAAATCAACC AGCATCAGTG ACTGATTATC AAAATGTATC TTTTACAAAC 
CTAACTGTTA ATACATCATG GCAGAAAAAA TCAGCTTTTA CTCGTACTGT TTCCCCTGGA 
TCTGTATCAC CGATTCATGG CCAGGGACAG GTTGTAGAAA ACCTAAAGGC GCAGGCCCTG 
TGTTCTTGGA CTGCAAAGAA GGATAACCAC TTGAACTTTT CAAAACATGA TATTATCACT 
GTGTTGGAAC AGCAAGAAAA TTGGTGGTTT GGAGAGGTGC ATGGAGGAAG AGGGTGGTTT 
CCAAAATCTT ATGTCAAAAT ACTCCCTGGG AGTGAAGTCA AGCGGGAAGA ACCAGAAGCT 
TTGTATGCAG CTGTAAACAA GAAACCTACC TCTGCAGCCT ACACAGGTGG AGAGGAATAT 
ATTGCACTTT ATCCATATTC AAGTGTAGAA CCTGGAGATT TGACTTTCAC TGAAGGTGAA 
GAAATATTGG TGACCCAGAA AGATGGAGAA TGGTGGACAG GAAGTATTGG AGATAGAACT 
GGAATTTTTC CATCAAATTA TGTCAAACCA AAAGATCAAG AGAGTTTTGG GAGTGCTAGC 
AAATCTGGAA CATCAAACAA GAAACCTGAG ATTGCTCAAG TAACTTCAGC ATATGTGGCT 
TCTGGTTCTG AACAACTTAG CCTTGCACCC GGGCAGTTAA TACTAATCCT AAAGAAAAAT 
TCAAGTGGGT GGTGGCAAGG GGAATTACAG GCCAGAGGAA AAAAGCGACA GAAAGGATGG 
TTTCCTGCCA GCCATGTTAA ACTTTTGGGT CCAAGTAGTG AAAGAACTAC ACCTGCCTTT 
CATCCTGTAT GTCAGGTGAT TGCTATGTAT GACTATGTAG CAAATAACGA GGATGAGCTG 
AATTTCTCCA AGGGACAACT TATCAATGTT TTGAACAAAG ATGATCCTGA CTGGTGGCAA 
GGAGAAATCA ACGGGGTGAC TGGTCTCTTT CCTTCAAACT ATGTTAAGAT GACAACAGAC 
TCCGATCCAA GTCAACAGTG GTGTGCTGAT CTCCAAAGCC TGGACACCAT GCAGCCAGTT 
GAGAGGAAGA GGCAGGGCTA TATTCACGAG CTGATTCAGA CCGAAGAGCG GTACGTGGAC 
GACCTTCAGC TCGTCGTGGA GGTTTTTCAG AAACGGATGG TGGAGTCGGG CTTTCTCAGT 
GAAGGAGAAA TGGCCTTGAT TTTTGTAAAC TGGAAAGAAC TCATCATGTC TAACACAAAG 
CTACTCAAGG CCTTGCGGGT ACGGAAGAAG ACCGGGGGAG AGAAGATGCC CGTGCAGATG 
ATTGGGGACA TCCTGGCAGC CGAGCTGTCC CACATGCAGG CTTATATCCG GTTCTGTAGC 
TGTCAGCTTA ATGGAGCAGC TCTGTTACAG CAGAAGACCG ATGAAGACAC GGATTTCAAG 
GAATTTTTAA AGAAGCTGGC ATCTGACCCT CGATGTAAAG GAATGCCCCT TTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG AATCACCCGC TACCCTCTGC TCATCAGAAG TATTCTGGAG 
AACACCCTGG AGAGTCATGT GGACCATTCC TCCCTAAAGC TGGCTCTTGA GCGGGCAGAG 
GAGCTTTGTT CCCAAGTTAA TGAAGGAGTT CGGGAAAAAG AAAATTCAGA CCGACTCGAG 
TGGATCCAGG CACACGTTCA GTGTGAAGGT CTTGCAGAGC AACTTATTTT CAACTCCCTC 
ACCAACTGCC TGGGGCCCCG GAAGCTTCTG CACAGTGGGA AACTATACAA GACCAAGAGC 
AACAAGGAAC TACACGGATT CCTCTTCAAT GACTTTCTGC TTCTTACCTA CATGGTCAAG 
CAGTTTGCTG TTTCCTCAGG TTCTGAGAAG CTTTTCAGCT CTAAGTCCAA TGCCCAATTC 
AAAATGTATA AAACGCCCAT TTTCCTGAAT GAAGTCCTGG TGAAACTGCC CACAGACCCT 
TCCAGCGATG AGCCTGTCTT CCACATTTCT CACATTGACC GGGTCTACAC CCTCCGAACA 
GACAACATTA ACGAGAGGAC TGCTTGGGTC CAGAAGATCA AGGCGGCATC TGAGCAGTAC 
ATTGACACGG AAAAGAAGAA ACGGGAGAAG GCTTATCAAG CTCGCTCTCA AAAGACTTCA 
GGCATTGGGC GTCTGATGGT GCACGTCATC GAAGCTACAG AATTAAAAGC CTGTAAACCG 
AATGGAAAGA GCAATCCATA CTGTGAAATC AGCATGGGCT CCCAGAGCTA TACCACCAGA 
ACCCTCCAGG ACACACTCAA TCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAC 
CTTTACCAAG ATGTGCTGTG TCTCACCATG TTTGACAGAG ACCAGTTTTC ACCAGATGAT 
TTCCTGGGTC GCACTGAAGT TCCAGTGGCA AAAATTCGCA CAGAACAGGA AAGCAAAGGC 
CCCACAACCC GCCGACTCCT GCTGCACGAG GTCCCCACCG GGGAGGTCTG GGTCCGCTTT 
GACCTGCAGC TTTTTGAGCA AAAAACTCTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252050.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107818.1 Felis catus intersectin 2 (ITSN2), transcript variant X2, mRNA.

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CloneID	OFb73977
Clone ID Related Accession (Same CDS sequence)	XM_023252050.1 , XM_023252051.1 , XM_023252052.1 , XM_019827831.2 , XM_023252049.1
Accession Version	XM_023252050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107818.1
CDS	141..5300
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252050.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252050.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252051.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107819.1 Felis catus intersectin 2 (ITSN2), transcript variant X4, mRNA.

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CloneID	OFb73977
Clone ID Related Accession (Same CDS sequence)	XM_023252050.1 , XM_023252051.1 , XM_023252052.1 , XM_019827831.2 , XM_023252049.1
Accession Version	XM_023252051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107819.1
CDS	57..5216
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252051.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252051.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252052.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107820.1 Felis catus intersectin 2 (ITSN2), transcript variant X5, mRNA.

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CloneID	OFb73977
Clone ID Related Accession (Same CDS sequence)	XM_023252050.1 , XM_023252051.1 , XM_023252052.1 , XM_019827831.2 , XM_023252049.1
Accession Version	XM_023252052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107820.1
CDS	136..5295
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252052.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252052.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827831.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683390.2 Felis catus intersectin 2 (ITSN2), transcript variant X3, mRNA.

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CloneID	OFb73977
Clone ID Related Accession (Same CDS sequence)	XM_023252050.1 , XM_023252051.1 , XM_023252052.1 , XM_019827831.2 , XM_023252049.1
Accession Version	XM_019827831.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019827831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683390.2
CDS	161..5320
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827831.2
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827831.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252049.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107817.1 Felis catus intersectin 2 (ITSN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb73977
Clone ID Related Accession (Same CDS sequence)	XM_023252050.1 , XM_023252051.1 , XM_023252052.1 , XM_019827831.2 , XM_023252049.1
Accession Version	XM_023252049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107817.1
CDS	136..5295
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252049.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252049.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGAAGC TGGCATCTGA CCCTCGATGT 
AAAGGAATGC CCCTTTCCAG CTTCCTGCTG AAGCCCATGC AGAGAATCAC CCGCTACCCT 
CTGCTCATCA GAAGTATTCT GGAGAACACC CTGGAGAGTC ATGTGGACCA TTCCTCCCTA 
AAGCTGGCTC TTGAGCGGGC AGAGGAGCTT TGTTCCCAAG TTAATGAAGG AGTTCGGGAA 
AAAGAAAATT CAGACCGACT CGAGTGGATC CAGGCACACG TTCAGTGTGA AGGTCTTGCA 
GAGCAACTTA TTTTCAACTC CCTCACCAAC TGCCTGGGGC CCCGGAAGCT TCTGCACAGT 
GGGAAACTAT ACAAGACCAA GAGCAACAAG GAACTACACG GATTCCTCTT CAATGACTTT 
CTGCTTCTTA CCTACATGGT CAAGCAGTTT GCTGTTTCCT CAGGTTCTGA GAAGCTTTTC 
AGCTCTAAGT CCAATGCCCA ATTCAAAATG TATAAAACGC CCATTTTCCT GAATGAAGTC 
CTGGTGAAAC TGCCCACAGA CCCTTCCAGC GATGAGCCTG TCTTCCACAT TTCTCACATT 
GACCGGGTCT ACACCCTCCG AACAGACAAC ATTAACGAGA GGACTGCTTG GGTCCAGAAG 
ATCAAGGCGG CATCTGAGCA GTACATTGAC ACGGAAAAGA AGAAACGGGA GAAGGCTTAT 
CAAGCTCGCT CTCAAAAGAC TTCAGGCATT GGGCGTCTGA TGGTGCACGT CATCGAAGCT 
ACAGAATTAA AAGCCTGTAA ACCGAATGGA AAGAGCAATC CATACTGTGA AATCAGCATG 
GGCTCCCAGA GCTATACCAC CAGAACCCTC CAGGACACAC TCAATCCCAA GTGGAACTTT 
AACTGCCAGT TCTTTATTAA GGACCTTTAC CAAGATGTGC TGTGTCTCAC CATGTTTGAC 
AGAGACCAGT TTTCACCAGA TGATTTCCTG GGTCGCACTG AAGTTCCAGT GGCAAAAATT 
CGCACAGAAC AGGAAAGCAA AGGCCCCACA ACCCGCCGAC TCCTGCTGCA CGAGGTCCCC 
ACCGGGGAGG TCTGGGTCCG CTTTGACCTG CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252053.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107821.1 Felis catus intersectin 2 (ITSN2), transcript variant X6, mRNA.

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CloneID	OFb73978
Clone ID Related Accession (Same CDS sequence)	XM_023252053.1
Accession Version	XM_023252053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTGAGCC AGCTACTGCT 
ATGGTGAATT ATAGAGCATT GTACCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT 
AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCGTAG GAGAACCTGG TTGGCTTTAT 
GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCCATCA 
GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC AGTATCTGCT 
ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT 
CGTACTGTTT CCCCTGGATC TGTATCACCG ATTCATGGCC AGGGACAGGT TGTAGAAAAC 
CTAAAGGCGC AGGCCCTGTG TTCTTGGACT GCAAAGAAGG ATAACCACTT GAACTTTTCA 
AAACATGATA TTATCACTGT GTTGGAACAG CAAGAAAATT GGTGGTTTGG AGAGGTGCAT 
GGAGGAAGAG GGTGGTTTCC AAAATCTTAT GTCAAAATAC TCCCTGGGAG TGAAGTCAAG 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAC 
ACAGGTGGAG AGGAATATAT TGCACTTTAT CCATATTCAA GTGTAGAACC TGGAGATTTG 
ACTTTCACTG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAATG GTGGACAGGA 
AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA ATCTGGAACA TCAAACAAGA AACCTGAGAT TGCTCAAGTA 
ACTTCAGCAT ATGTGGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCCGG GCAGTTAATA 
CTAATCCTAA AGAAAAATTC AAGTGGGTGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAACTACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGTAGCA 
AATAACGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT 
GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT 
GTTAAGATGA CAACAGACTC CGATCCAAGT CAACAGTGGT GTGCTGATCT CCAAAGCCTG 
GACACCATGC AGCCAGTTGA GAGGAAGAGG CAGGGCTATA TTCACGAGCT GATTCAGACC 
GAAGAGCGGT ACGTGGACGA CCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGTG 
GAGTCGGGCT TTCTCAGTGA AGGAGAAATG GCCTTGATTT TTGTAAACTG GAAAGAACTC 
ATCATGTCTA ACACAAAGCT ACTCAAGGCC TTGCGGGTAC GGAAGAAGAC CGGGGGAGAG 
AAGATGCCCG TGCAGATGAT TGGGGACATC CTGGCAGCCG AGCTGTCCCA CATGCAGGCT 
TATATCCGGT TCTGTAGCTG TCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACCGAT 
GAAGACACGG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG ATGTAAAGGA 
ATGCCCCTTT CCAGCTTCCT GCTGAAGCCC ATGCAGAGAA TCACCCGCTA CCCTCTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AGTCATGTGG ACCATTCCTC CCTAAAGCTG 
GCTCTTGAGC GGGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA 
AATTCAGACC GACTCGAGTG GATCCAGGCA CACGTTCAGT GTGAAGGTCT TGCAGAGCAA 
CTTATTTTCA ACTCCCTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA 
CTATACAAGA CCAAGAGCAA CAAGGAACTA CACGGATTCC TCTTCAATGA CTTTCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCAGGTT CTGAGAAGCT TTTCAGCTCT 
AAGTCCAATG CCCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCCTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCTCA CATTGACCGG 
GTCTACACCC TCCGAACAGA CAACATTAAC GAGAGGACTG CTTGGGTCCA GAAGATCAAG 
GCGGCATCTG AGCAGTACAT TGACACGGAA AAGAAGAAAC GGGAGAAGGC TTATCAAGCT 
CGCTCTCAAA AGACTTCAGG CATTGGGCGT CTGATGGTGC ACGTCATCGA AGCTACAGAA 
TTAAAAGCCT GTAAACCGAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTATA CCACCAGAAC CCTCCAGGAC ACACTCAATC CCAAGTGGAA CTTTAACTGC 
CAGTTCTTTA TTAAGGACCT TTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGC ACTGAAGTTC CAGTGGCAAA AATTCGCACA 
GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTCCTGC TGCACGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107821.1
CDS	136..5289
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252053.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252053.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTGAGCC AGCTACTGCT 
ATGGTGAATT ATAGAGCATT GTACCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT 
AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCGTAG GAGAACCTGG TTGGCTTTAT 
GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCCATCA 
GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC AGTATCTGCT 
ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT 
CGTACTGTTT CCCCTGGATC TGTATCACCG ATTCATGGCC AGGGACAGGT TGTAGAAAAC 
CTAAAGGCGC AGGCCCTGTG TTCTTGGACT GCAAAGAAGG ATAACCACTT GAACTTTTCA 
AAACATGATA TTATCACTGT GTTGGAACAG CAAGAAAATT GGTGGTTTGG AGAGGTGCAT 
GGAGGAAGAG GGTGGTTTCC AAAATCTTAT GTCAAAATAC TCCCTGGGAG TGAAGTCAAG 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAC 
ACAGGTGGAG AGGAATATAT TGCACTTTAT CCATATTCAA GTGTAGAACC TGGAGATTTG 
ACTTTCACTG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAATG GTGGACAGGA 
AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA ATCTGGAACA TCAAACAAGA AACCTGAGAT TGCTCAAGTA 
ACTTCAGCAT ATGTGGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCCGG GCAGTTAATA 
CTAATCCTAA AGAAAAATTC AAGTGGGTGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAACTACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGTAGCA 
AATAACGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT 
GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT 
GTTAAGATGA CAACAGACTC CGATCCAAGT CAACAGTGGT GTGCTGATCT CCAAAGCCTG 
GACACCATGC AGCCAGTTGA GAGGAAGAGG CAGGGCTATA TTCACGAGCT GATTCAGACC 
GAAGAGCGGT ACGTGGACGA CCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGTG 
GAGTCGGGCT TTCTCAGTGA AGGAGAAATG GCCTTGATTT TTGTAAACTG GAAAGAACTC 
ATCATGTCTA ACACAAAGCT ACTCAAGGCC TTGCGGGTAC GGAAGAAGAC CGGGGGAGAG 
AAGATGCCCG TGCAGATGAT TGGGGACATC CTGGCAGCCG AGCTGTCCCA CATGCAGGCT 
TATATCCGGT TCTGTAGCTG TCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACCGAT 
GAAGACACGG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG ATGTAAAGGA 
ATGCCCCTTT CCAGCTTCCT GCTGAAGCCC ATGCAGAGAA TCACCCGCTA CCCTCTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AGTCATGTGG ACCATTCCTC CCTAAAGCTG 
GCTCTTGAGC GGGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA 
AATTCAGACC GACTCGAGTG GATCCAGGCA CACGTTCAGT GTGAAGGTCT TGCAGAGCAA 
CTTATTTTCA ACTCCCTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA 
CTATACAAGA CCAAGAGCAA CAAGGAACTA CACGGATTCC TCTTCAATGA CTTTCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCAGGTT CTGAGAAGCT TTTCAGCTCT 
AAGTCCAATG CCCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCCTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCTCA CATTGACCGG 
GTCTACACCC TCCGAACAGA CAACATTAAC GAGAGGACTG CTTGGGTCCA GAAGATCAAG 
GCGGCATCTG AGCAGTACAT TGACACGGAA AAGAAGAAAC GGGAGAAGGC TTATCAAGCT 
CGCTCTCAAA AGACTTCAGG CATTGGGCGT CTGATGGTGC ACGTCATCGA AGCTACAGAA 
TTAAAAGCCT GTAAACCGAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTATA CCACCAGAAC CCTCCAGGAC ACACTCAATC CCAAGTGGAA CTTTAACTGC 
CAGTTCTTTA TTAAGGACCT TTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGC ACTGAAGTTC CAGTGGCAAA AATTCGCACA 
GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTCCTGC TGCACGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252054.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107822.1 Felis catus intersectin 2 (ITSN2), transcript variant X7, mRNA.

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CloneID	OFb73979
Clone ID Related Accession (Same CDS sequence)	XM_023252054.1
Accession Version	XM_023252054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGGA ACTGAGAGAA 
AGCTACAATA CACAGCAGTT AGCCCTTGAA CAACTTTATA AGATCAAACG TGACAAGTTG 
AAGGAAATTG AAAGAAAAAG GTCAGAGCTA ATACAGAAGA AGAAACTAGA AGATGAGGCT 
GTAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAGAG AAAACCTTAG AAAAGAGGAA 
GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAACACAAG AAAAAGTTCA AGAAGAGGAA 
CGGAAGGCTG AGGAGAAACC ATGTAAGGGT GAGCCAGCTA CTGCTATGGT GAATTATAGA 
GCATTGTACC GCTTTGAAGC CAGGAGCCAT GATGAGATGA GTTTTAATTC CGGGGATATA 
ATACAGGTTG ATGAAAAAAC CGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA 
AATTTTGGCT GGTTTCCTTG CAACTATGTA GAAAAAATGC CATCAGGTGA AAAATCTGTG 
TCTCCTAAGA AGGCCTTACT TCCCCCTACA GTATCAGTAT CTGCTACCTC AACTTCTTCT 
GAATCACTTT CTTCAAATCA ACCAGCATCA GTGACTGATT ATCAAAATGT ATCTTTTACA 
AACCTAACTG TTAATACATC ATGGCAGAAA AAATCAGCTT TTACTCGTAC TGTTTCCCCT 
GGATCTGTAT CACCGATTCA TGGCCAGGGA CAGGTTGTAG AAAACCTAAA GGCGCAGGCC 
CTGTGTTCTT GGACTGCAAA GAAGGATAAC CACTTGAACT TTTCAAAACA TGATATTATC 
ACTGTGTTGG AACAGCAAGA AAATTGGTGG TTTGGAGAGG TGCATGGAGG AAGAGGGTGG 
TTTCCAAAAT CTTATGTCAA AATACTCCCT GGGAGTGAAG TCAAGCGGGA AGAACCAGAA 
GCTTTGTATG CAGCTGTAAA CAAGAAACCT ACCTCTGCAG CCTACACAGG TGGAGAGGAA 
TATATTGCAC TTTATCCATA TTCAAGTGTA GAACCTGGAG ATTTGACTTT CACTGAAGGT 
GAAGAAATAT TGGTGACCCA GAAAGATGGA GAATGGTGGA CAGGAAGTAT TGGAGATAGA 
ACTGGAATTT TTCCATCAAA TTATGTCAAA CCAAAAGATC AAGAGAGTTT TGGGAGTGCT 
AGCAAATCTG GAACATCAAA CAAGAAACCT GAGATTGCTC AAGTAACTTC AGCATATGTG 
GCTTCTGGTT CTGAACAACT TAGCCTTGCA CCCGGGCAGT TAATACTAAT CCTAAAGAAA 
AATTCAAGTG GGTGGTGGCA AGGGGAATTA CAGGCCAGAG GAAAAAAGCG ACAGAAAGGA 
TGGTTTCCTG CCAGCCATGT TAAACTTTTG GGTCCAAGTA GTGAAAGAAC TACACCTGCC 
TTTCATCCTG TATGTCAGGT GATTGCTATG TATGACTATG TAGCAAATAA CGAGGATGAG 
CTGAATTTCT CCAAGGGACA ACTTATCAAT GTTTTGAACA AAGATGATCC TGACTGGTGG 
CAAGGAGAAA TCAACGGGGT GACTGGTCTC TTTCCTTCAA ACTATGTTAA GATGACAACA 
GACTCCGATC CAAGTCAACA GTGGTGTGCT GATCTCCAAA GCCTGGACAC CATGCAGCCA 
GTTGAGAGGA AGAGGCAGGG CTATATTCAC GAGCTGATTC AGACCGAAGA GCGGTACGTG 
GACGACCTTC AGCTCGTCGT GGAGGTTTTT CAGAAACGGA TGGTGGAGTC GGGCTTTCTC 
AGTGAAGGAG AAATGGCCTT GATTTTTGTA AACTGGAAAG AACTCATCAT GTCTAACACA 
AAGCTACTCA AGGCCTTGCG GGTACGGAAG AAGACCGGGG GAGAGAAGAT GCCCGTGCAG 
ATGATTGGGG ACATCCTGGC AGCCGAGCTG TCCCACATGC AGGCTTATAT CCGGTTCTGT 
AGCTGTCAGC TTAATGGAGC AGCTCTGTTA CAGCAGAAGA CCGATGAAGA CACGGATTTC 
AAGGAATTTT TAAAGAAGCT GGCATCTGAC CCTCGATGTA AAGGAATGCC CCTTTCCAGC 
TTCCTGCTGA AGCCCATGCA GAGAATCACC CGCTACCCTC TGCTCATCAG AAGTATTCTG 
GAGAACACCC TGGAGAGTCA TGTGGACCAT TCCTCCCTAA AGCTGGCTCT TGAGCGGGCA 
GAGGAGCTTT GTTCCCAAGT TAATGAAGGA GTTCGGGAAA AAGAAAATTC AGACCGACTC 
GAGTGGATCC AGGCACACGT TCAGTGTGAA GGTCTTGCAG AGCAACTTAT TTTCAACTCC 
CTCACCAACT GCCTGGGGCC CCGGAAGCTT CTGCACAGTG GGAAACTATA CAAGACCAAG 
AGCAACAAGG AACTACACGG ATTCCTCTTC AATGACTTTC TGCTTCTTAC CTACATGGTC 
AAGCAGTTTG CTGTTTCCTC AGGTTCTGAG AAGCTTTTCA GCTCTAAGTC CAATGCCCAA 
TTCAAAATGT ATAAAACGCC CATTTTCCTG AATGAAGTCC TGGTGAAACT GCCCACAGAC 
CCTTCCAGCG ATGAGCCTGT CTTCCACATT TCTCACATTG ACCGGGTCTA CACCCTCCGA 
ACAGACAACA TTAACGAGAG GACTGCTTGG GTCCAGAAGA TCAAGGCGGC ATCTGAGCAG 
TACATTGACA CGGAAAAGAA GAAACGGGAG AAGGCTTATC AAGCTCGCTC TCAAAAGACT 
TCAGGCATTG GGCGTCTGAT GGTGCACGTC ATCGAAGCTA CAGAATTAAA AGCCTGTAAA 
CCGAATGGAA AGAGCAATCC ATACTGTGAA ATCAGCATGG GCTCCCAGAG CTATACCACC 
AGAACCCTCC AGGACACACT CAATCCCAAG TGGAACTTTA ACTGCCAGTT CTTTATTAAG 
GACCTTTACC AAGATGTGCT GTGTCTCACC ATGTTTGACA GAGACCAGTT TTCACCAGAT 
GATTTCCTGG GTCGCACTGA AGTTCCAGTG GCAAAAATTC GCACAGAACA GGAAAGCAAA 
GGCCCCACAA CCCGCCGACT CCTGCTGCAC GAGGTCCCCA CCGGGGAGGT CTGGGTCCGC 
TTTGACCTGC AGCTTTTTGA GCAAAAAACT CTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107822.1
CDS	136..5214
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252054.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252054.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGGA ACTGAGAGAA 
AGCTACAATA CACAGCAGTT AGCCCTTGAA CAACTTTATA AGATCAAACG TGACAAGTTG 
AAGGAAATTG AAAGAAAAAG GTCAGAGCTA ATACAGAAGA AGAAACTAGA AGATGAGGCT 
GTAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAGAG AAAACCTTAG AAAAGAGGAA 
GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAACACAAG AAAAAGTTCA AGAAGAGGAA 
CGGAAGGCTG AGGAGAAACC ATGTAAGGGT GAGCCAGCTA CTGCTATGGT GAATTATAGA 
GCATTGTACC GCTTTGAAGC CAGGAGCCAT GATGAGATGA GTTTTAATTC CGGGGATATA 
ATACAGGTTG ATGAAAAAAC CGTAGGAGAA CCTGGTTGGC TTTATGGTAG TTTTCAAGGA 
AATTTTGGCT GGTTTCCTTG CAACTATGTA GAAAAAATGC CATCAGGTGA AAAATCTGTG 
TCTCCTAAGA AGGCCTTACT TCCCCCTACA GTATCAGTAT CTGCTACCTC AACTTCTTCT 
GAATCACTTT CTTCAAATCA ACCAGCATCA GTGACTGATT ATCAAAATGT ATCTTTTACA 
AACCTAACTG TTAATACATC ATGGCAGAAA AAATCAGCTT TTACTCGTAC TGTTTCCCCT 
GGATCTGTAT CACCGATTCA TGGCCAGGGA CAGGTTGTAG AAAACCTAAA GGCGCAGGCC 
CTGTGTTCTT GGACTGCAAA GAAGGATAAC CACTTGAACT TTTCAAAACA TGATATTATC 
ACTGTGTTGG AACAGCAAGA AAATTGGTGG TTTGGAGAGG TGCATGGAGG AAGAGGGTGG 
TTTCCAAAAT CTTATGTCAA AATACTCCCT GGGAGTGAAG TCAAGCGGGA AGAACCAGAA 
GCTTTGTATG CAGCTGTAAA CAAGAAACCT ACCTCTGCAG CCTACACAGG TGGAGAGGAA 
TATATTGCAC TTTATCCATA TTCAAGTGTA GAACCTGGAG ATTTGACTTT CACTGAAGGT 
GAAGAAATAT TGGTGACCCA GAAAGATGGA GAATGGTGGA CAGGAAGTAT TGGAGATAGA 
ACTGGAATTT TTCCATCAAA TTATGTCAAA CCAAAAGATC AAGAGAGTTT TGGGAGTGCT 
AGCAAATCTG GAACATCAAA CAAGAAACCT GAGATTGCTC AAGTAACTTC AGCATATGTG 
GCTTCTGGTT CTGAACAACT TAGCCTTGCA CCCGGGCAGT TAATACTAAT CCTAAAGAAA 
AATTCAAGTG GGTGGTGGCA AGGGGAATTA CAGGCCAGAG GAAAAAAGCG ACAGAAAGGA 
TGGTTTCCTG CCAGCCATGT TAAACTTTTG GGTCCAAGTA GTGAAAGAAC TACACCTGCC 
TTTCATCCTG TATGTCAGGT GATTGCTATG TATGACTATG TAGCAAATAA CGAGGATGAG 
CTGAATTTCT CCAAGGGACA ACTTATCAAT GTTTTGAACA AAGATGATCC TGACTGGTGG 
CAAGGAGAAA TCAACGGGGT GACTGGTCTC TTTCCTTCAA ACTATGTTAA GATGACAACA 
GACTCCGATC CAAGTCAACA GTGGTGTGCT GATCTCCAAA GCCTGGACAC CATGCAGCCA 
GTTGAGAGGA AGAGGCAGGG CTATATTCAC GAGCTGATTC AGACCGAAGA GCGGTACGTG 
GACGACCTTC AGCTCGTCGT GGAGGTTTTT CAGAAACGGA TGGTGGAGTC GGGCTTTCTC 
AGTGAAGGAG AAATGGCCTT GATTTTTGTA AACTGGAAAG AACTCATCAT GTCTAACACA 
AAGCTACTCA AGGCCTTGCG GGTACGGAAG AAGACCGGGG GAGAGAAGAT GCCCGTGCAG 
ATGATTGGGG ACATCCTGGC AGCCGAGCTG TCCCACATGC AGGCTTATAT CCGGTTCTGT 
AGCTGTCAGC TTAATGGAGC AGCTCTGTTA CAGCAGAAGA CCGATGAAGA CACGGATTTC 
AAGGAATTTT TAAAGAAGCT GGCATCTGAC CCTCGATGTA AAGGAATGCC CCTTTCCAGC 
TTCCTGCTGA AGCCCATGCA GAGAATCACC CGCTACCCTC TGCTCATCAG AAGTATTCTG 
GAGAACACCC TGGAGAGTCA TGTGGACCAT TCCTCCCTAA AGCTGGCTCT TGAGCGGGCA 
GAGGAGCTTT GTTCCCAAGT TAATGAAGGA GTTCGGGAAA AAGAAAATTC AGACCGACTC 
GAGTGGATCC AGGCACACGT TCAGTGTGAA GGTCTTGCAG AGCAACTTAT TTTCAACTCC 
CTCACCAACT GCCTGGGGCC CCGGAAGCTT CTGCACAGTG GGAAACTATA CAAGACCAAG 
AGCAACAAGG AACTACACGG ATTCCTCTTC AATGACTTTC TGCTTCTTAC CTACATGGTC 
AAGCAGTTTG CTGTTTCCTC AGGTTCTGAG AAGCTTTTCA GCTCTAAGTC CAATGCCCAA 
TTCAAAATGT ATAAAACGCC CATTTTCCTG AATGAAGTCC TGGTGAAACT GCCCACAGAC 
CCTTCCAGCG ATGAGCCTGT CTTCCACATT TCTCACATTG ACCGGGTCTA CACCCTCCGA 
ACAGACAACA TTAACGAGAG GACTGCTTGG GTCCAGAAGA TCAAGGCGGC ATCTGAGCAG 
TACATTGACA CGGAAAAGAA GAAACGGGAG AAGGCTTATC AAGCTCGCTC TCAAAAGACT 
TCAGGCATTG GGCGTCTGAT GGTGCACGTC ATCGAAGCTA CAGAATTAAA AGCCTGTAAA 
CCGAATGGAA AGAGCAATCC ATACTGTGAA ATCAGCATGG GCTCCCAGAG CTATACCACC 
AGAACCCTCC AGGACACACT CAATCCCAAG TGGAACTTTA ACTGCCAGTT CTTTATTAAG 
GACCTTTACC AAGATGTGCT GTGTCTCACC ATGTTTGACA GAGACCAGTT TTCACCAGAT 
GATTTCCTGG GTCGCACTGA AGTTCCAGTG GCAAAAATTC GCACAGAACA GGAAAGCAAA 
GGCCCCACAA CCCGCCGACT CCTGCTGCAC GAGGTCCCCA CCGGGGAGGT CTGGGTCCGC 
TTTGACCTGC AGCTTTTTGA GCAAAAAACT CTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252055.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107823.1 Felis catus intersectin 2 (ITSN2), transcript variant X8, mRNA.

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CloneID	OFb73980
Clone ID Related Accession (Same CDS sequence)	XM_023252055.1
Accession Version	XM_023252055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGATTC TGGAGAACAC CCTGGAGAGT 
CATGTGGACC ATTCCTCCCT AAAGCTGGCT CTTGAGCGGG CAGAGGAGCT TTGTTCCCAA 
GTTAATGAAG GAGTTCGGGA AAAAGAAAAT TCAGACCGAC TCGAGTGGAT CCAGGCACAC 
GTTCAGTGTG AAGGTCTTGC AGAGCAACTT ATTTTCAACT CCCTCACCAA CTGCCTGGGG 
CCCCGGAAGC TTCTGCACAG TGGGAAACTA TACAAGACCA AGAGCAACAA GGAACTACAC 
GGATTCCTCT TCAATGACTT TCTGCTTCTT ACCTACATGG TCAAGCAGTT TGCTGTTTCC 
TCAGGTTCTG AGAAGCTTTT CAGCTCTAAG TCCAATGCCC AATTCAAAAT GTATAAAACG 
CCCATTTTCC TGAATGAAGT CCTGGTGAAA CTGCCCACAG ACCCTTCCAG CGATGAGCCT 
GTCTTCCACA TTTCTCACAT TGACCGGGTC TACACCCTCC GAACAGACAA CATTAACGAG 
AGGACTGCTT GGGTCCAGAA GATCAAGGCG GCATCTGAGC AGTACATTGA CACGGAAAAG 
AAGAAACGGG AGAAGGCTTA TCAAGCTCGC TCTCAAAAGA CTTCAGGCAT TGGGCGTCTG 
ATGGTGCACG TCATCGAAGC TACAGAATTA AAAGCCTGTA AACCGAATGG AAAGAGCAAT 
CCATACTGTG AAATCAGCAT GGGCTCCCAG AGCTATACCA CCAGAACCCT CCAGGACACA 
CTCAATCCCA AGTGGAACTT TAACTGCCAG TTCTTTATTA AGGACCTTTA CCAAGATGTG 
CTGTGTCTCA CCATGTTTGA CAGAGACCAG TTTTCACCAG ATGATTTCCT GGGTCGCACT 
GAAGTTCCAG TGGCAAAAAT TCGCACAGAA CAGGAAAGCA AAGGCCCCAC AACCCGCCGA 
CTCCTGCTGC ACGAGGTCCC CACCGGGGAG GTCTGGGTCC GCTTTGACCT GCAGCTTTTT 
GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107823.1
CDS	136..5196
Translation	MMAQFPTTMNEQKRSVRHIFTTRDSNSGVFCTSRIILARVFFNHGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252055.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252055.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC 
TTCTTTAATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT 
GACAAGCAGT TTGATAACCT CAAACCCTCA GGAGGTTACA TAACAGGTGA TCAAGCACGT 
ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA 
GACCTGAACA AGGATGGGAA GATGGATCAG CAAGAATTCT CCATAGCGAT GAAGCTCATC 
AAACTGAAGC TACAAGGCCA GCAGCTGCCC GTGGTCCTCC CTCCTATTAT GAAACAACCT 
CCTATGTTCT CTCCATTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC 
ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGGCAACCC CCTTACCCTC TGCGACTTCA 
GGGACCACCC TTCCTCCCTT AATGATGCCT GCTCCCCTAG TGCCTTCTGT TAGCACATCG 
TCACTACCAA ATGGAACCGC TGGCCTCATT CAGCCGTTAT CCATTCCTTA TTCTTCTTCA 
ACATTGCCTC ATACGTCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA 
CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACTTCCTC AACTGCTTCA 
CTCTCTGGAA ACTCGCCCAA GACTGGGACC TTAGAGTGGG CGGTTCCTCA GCCTTCAAGG 
TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGATA TCTCTCAGGT 
TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT 
TGGACCCTTG CTGACATTGA TGGCGACGGA CAGCTGAAAG CTGAAGAGTT CATCCTTGCG 
ATGCATCTCA CTGACATGGC CAAAGCTGGA CAGCCCTTGC CACTGACGTT GCCTCCTGAA 
CTTGTCCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT CCATTAATGG AACTCTGCCT 
TCCTATCAGA AAATTCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAAGAC 
AAGCGGAAGG CCAACTACGA GCGAGGGAAC GTGGAACTGG AGAAGCGGCG CCAAGCCCTG 
ATGGAGCAGC AGCAGAGGGA GGCAGAGCGA AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG 
CGAAAACAGA GAGAATTACA GGAACAAGAA TGGAAGAAAC AACTTGAATT AGAAAAACGC 
TTGGAGAAGC AGAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA 
AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT 
CGTCGCCAGG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC 
TCTAAAAAGA AGAGTCTTCA TCTCGAGTTG GAAGCACTGA ATGGCAAACA CCAGCAGATC 
TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT 
CTAGATAAAC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG 
GATTATCAAA ATAAACTTAT CTATTTGGTA CCGGAGAAGC AATTATTAAA TGAAAGAATT 
AAGAACATGC AGCTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA 
TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA 
ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAATC AATTAAAGTG TGGGAATATG 
GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC 
TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAACTTTAT 
AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAG 
AAGAAACTAG AAGATGAGGC TGTAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGA 
GAAAACCTTA GAAAAGAGGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAACACAA 
GAAAAAGTTC AAGAAGAGGA ACGGAAGGCT GAGGAGAAAC CATGTAAGGG TGAGCCAGCT 
ACTGCTATGG TGAATTATAG AGCATTGTAC CGCTTTGAAG CCAGGAGCCA TGATGAGATG 
AGTTTTAATT CCGGGGATAT AATACAGGTT GATGAAAAAA CCGTAGGAGA ACCTGGTTGG 
CTTTATGGTA GTTTTCAAGG AAATTTTGGC TGGTTTCCTT GCAACTATGT AGAAAAAATG 
CCATCAGGTG AAAAATCTGT GTCTCCTAAG AAGGCCTTAC TTCCCCCTAC AGTATCAGTA 
TCTGCTACCT CAACTTCTTC TGAATCACTT TCTTCAAATC AACCAGCATC AGTGACTGAT 
TATCAAAATG TATCTTTTAC AAACCTAACT GTTAATACAT CATGGCAGAA AAAATCAGCT 
TTTACTCGTA CTGTTTCCCC TGGATCTGTA TCACCGATTC ATGGCCAGGG ACAGGTTGTA 
GAAAACCTAA AGGCGCAGGC CCTGTGTTCT TGGACTGCAA AGAAGGATAA CCACTTGAAC 
TTTTCAAAAC ATGATATTAT CACTGTGTTG GAACAGCAAG AAAATTGGTG GTTTGGAGAG 
GTGCATGGAG GAAGAGGGTG GTTTCCAAAA TCTTATGTCA AAATACTCCC TGGGAGTGAA 
GTCAAGCGGG AAGAACCAGA AGCTTTGTAT GCAGCTGTAA ACAAGAAACC TACCTCTGCA 
GCCTACACAG GTGGAGAGGA ATATATTGCA CTTTATCCAT ATTCAAGTGT AGAACCTGGA 
GATTTGACTT TCACTGAAGG TGAAGAAATA TTGGTGACCC AGAAAGATGG AGAATGGTGG 
ACAGGAAGTA TTGGAGATAG AACTGGAATT TTTCCATCAA ATTATGTCAA ACCAAAAGAT 
CAAGAGAGTT TTGGGAGTGC TAGCAAATCT GGAACATCAA ACAAGAAACC TGAGATTGCT 
CAAGTAACTT CAGCATATGT GGCTTCTGGT TCTGAACAAC TTAGCCTTGC ACCCGGGCAG 
TTAATACTAA TCCTAAAGAA AAATTCAAGT GGGTGGTGGC AAGGGGAATT ACAGGCCAGA 
GGAAAAAAGC GACAGAAAGG ATGGTTTCCT GCCAGCCATG TTAAACTTTT GGGTCCAAGT 
AGTGAAAGAA CTACACCTGC CTTTCATCCT GTATGTCAGG TGATTGCTAT GTATGACTAT 
GTAGCAAATA ACGAGGATGA GCTGAATTTC TCCAAGGGAC AACTTATCAA TGTTTTGAAC 
AAAGATGATC CTGACTGGTG GCAAGGAGAA ATCAACGGGG TGACTGGTCT CTTTCCTTCA 
AACTATGTTA AGATGACAAC AGACTCCGAT CCAAGTCAAC AGTGGTGTGC TGATCTCCAA 
AGCCTGGACA CCATGCAGCC AGTTGAGAGG AAGAGGCAGG GCTATATTCA CGAGCTGATT 
CAGACCGAAG AGCGGTACGT GGACGACCTT CAGCTCGTCG TGGAGGTTTT TCAGAAACGG 
ATGGTGGAGT CGGGCTTTCT CAGTGAAGGA GAAATGGCCT TGATTTTTGT AAACTGGAAA 
GAACTCATCA TGTCTAACAC AAAGCTACTC AAGGCCTTGC GGGTACGGAA GAAGACCGGG 
GGAGAGAAGA TGCCCGTGCA GATGATTGGG GACATCCTGG CAGCCGAGCT GTCCCACATG 
CAGGCTTATA TCCGGTTCTG TAGCTGTCAG CTTAATGGAG CAGCTCTGTT ACAGCAGAAG 
ACCGATGAAG ACACGGATTT CAAGGAATTT TTAAAGATTC TGGAGAACAC CCTGGAGAGT 
CATGTGGACC ATTCCTCCCT AAAGCTGGCT CTTGAGCGGG CAGAGGAGCT TTGTTCCCAA 
GTTAATGAAG GAGTTCGGGA AAAAGAAAAT TCAGACCGAC TCGAGTGGAT CCAGGCACAC 
GTTCAGTGTG AAGGTCTTGC AGAGCAACTT ATTTTCAACT CCCTCACCAA CTGCCTGGGG 
CCCCGGAAGC TTCTGCACAG TGGGAAACTA TACAAGACCA AGAGCAACAA GGAACTACAC 
GGATTCCTCT TCAATGACTT TCTGCTTCTT ACCTACATGG TCAAGCAGTT TGCTGTTTCC 
TCAGGTTCTG AGAAGCTTTT CAGCTCTAAG TCCAATGCCC AATTCAAAAT GTATAAAACG 
CCCATTTTCC TGAATGAAGT CCTGGTGAAA CTGCCCACAG ACCCTTCCAG CGATGAGCCT 
GTCTTCCACA TTTCTCACAT TGACCGGGTC TACACCCTCC GAACAGACAA CATTAACGAG 
AGGACTGCTT GGGTCCAGAA GATCAAGGCG GCATCTGAGC AGTACATTGA CACGGAAAAG 
AAGAAACGGG AGAAGGCTTA TCAAGCTCGC TCTCAAAAGA CTTCAGGCAT TGGGCGTCTG 
ATGGTGCACG TCATCGAAGC TACAGAATTA AAAGCCTGTA AACCGAATGG AAAGAGCAAT 
CCATACTGTG AAATCAGCAT GGGCTCCCAG AGCTATACCA CCAGAACCCT CCAGGACACA 
CTCAATCCCA AGTGGAACTT TAACTGCCAG TTCTTTATTA AGGACCTTTA CCAAGATGTG 
CTGTGTCTCA CCATGTTTGA CAGAGACCAG TTTTCACCAG ATGATTTCCT GGGTCGCACT 
GAAGTTCCAG TGGCAAAAAT TCGCACAGAA CAGGAAAGCA AAGGCCCCAC AACCCGCCGA 
CTCCTGCTGC ACGAGGTCCC CACCGGGGAG GTCTGGGTCC GCTTTGACCT GCAGCTTTTT 
GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252057.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107825.1 Felis catus intersectin 2 (ITSN2), transcript variant X10, mRNA.

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CloneID	OFb73981
Clone ID Related Accession (Same CDS sequence)	XM_023252057.1 , XM_023252056.1 , XM_019827835.2
Accession Version	XM_023252057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTAAGGGTG AGCCAGCTAC TGCTATGGTG AATTATAGAG CATTGTACCG CTTTGAAGCC 
AGGAGCCATG ATGAGATGAG TTTTAATTCC GGGGATATAA TACAGGTTGA TGAAAAAACC 
GTAGGAGAAC CTGGTTGGCT TTATGGTAGT TTTCAAGGAA ATTTTGGCTG GTTTCCTTGC 
AACTATGTAG AAAAAATGCC ATCAGGTGAA AAATCTGTGT CTCCTAAGAA GGCCTTACTT 
CCCCCTACAG TATCAGTATC TGCTACCTCA ACTTCTTCTG AATCACTTTC TTCAAATCAA 
CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTACAA ACCTAACTGT TAATACATCA 
TGGCAGAAAA AATCAGCTTT TACTCGTACT GTTTCCCCTG GATCTGTATC ACCGATTCAT 
GGCCAGGGAC AGGTTGTAGA AAACCTAAAG GCGCAGGCCC TGTGTTCTTG GACTGCAAAG 
AAGGATAACC ACTTGAACTT TTCAAAACAT GATATTATCA CTGTGTTGGA ACAGCAAGAA 
AATTGGTGGT TTGGAGAGGT GCATGGAGGA AGAGGGTGGT TTCCAAAATC TTATGTCAAA 
ATACTCCCTG GGAGTGAAGT CAAGCGGGAA GAACCAGAAG CTTTGTATGC AGCTGTAAAC 
AAGAAACCTA CCTCTGCAGC CTACACAGGT GGAGAGGAAT ATATTGCACT TTATCCATAT 
TCAAGTGTAG AACCTGGAGA TTTGACTTTC ACTGAAGGTG AAGAAATATT GGTGACCCAG 
AAAGATGGAG AATGGTGGAC AGGAAGTATT GGAGATAGAA CTGGAATTTT TCCATCAAAT 
TATGTCAAAC CAAAAGATCA AGAGAGTTTT GGGAGTGCTA GCAAATCTGG AACATCAAAC 
AAGAAACCTG AGATTGCTCA AGTAACTTCA GCATATGTGG CTTCTGGTTC TGAACAACTT 
AGCCTTGCAC CCGGGCAGTT AATACTAATC CTAAAGAAAA ATTCAAGTGG GTGGTGGCAA 
GGGGAATTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT GGTTTCCTGC CAGCCATGTT 
AAACTTTTGG GTCCAAGTAG TGAAAGAACT ACACCTGCCT TTCATCCTGT ATGTCAGGTG 
ATTGCTATGT ATGACTATGT AGCAAATAAC GAGGATGAGC TGAATTTCTC CAAGGGACAA 
CTTATCAATG TTTTGAACAA AGATGATCCT GACTGGTGGC AAGGAGAAAT CAACGGGGTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCCGATCC AAGTCAACAG 
TGGTGTGCTG ATCTCCAAAG CCTGGACACC ATGCAGCCAG TTGAGAGGAA GAGGCAGGGC 
TATATTCACG AGCTGATTCA GACCGAAGAG CGGTACGTGG ACGACCTTCA GCTCGTCGTG 
GAGGTTTTTC AGAAACGGAT GGTGGAGTCG GGCTTTCTCA GTGAAGGAGA AATGGCCTTG 
ATTTTTGTAA ACTGGAAAGA ACTCATCATG TCTAACACAA AGCTACTCAA GGCCTTGCGG 
GTACGGAAGA AGACCGGGGG AGAGAAGATG CCCGTGCAGA TGATTGGGGA CATCCTGGCA 
GCCGAGCTGT CCCACATGCA GGCTTATATC CGGTTCTGTA GCTGTCAGCT TAATGGAGCA 
GCTCTGTTAC AGCAGAAGAC CGATGAAGAC ACGGATTTCA AGGAATTTTT AAAGAAGCTG 
GCATCTGACC CTCGATGTAA AGGAATGCCC CTTTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGAATCACCC GCTACCCTCT GCTCATCAGA AGTATTCTGG AGAACACCCT GGAGAGTCAT 
GTGGACCATT CCTCCCTAAA GCTGGCTCTT GAGCGGGCAG AGGAGCTTTG TTCCCAAGTT 
AATGAAGGAG TTCGGGAAAA AGAAAATTCA GACCGACTCG AGTGGATCCA GGCACACGTT 
CAGTGTGAAG GTCTTGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGGCCC 
CGGAAGCTTC TGCACAGTGG GAAACTATAC AAGACCAAGA GCAACAAGGA ACTACACGGA 
TTCCTCTTCA ATGACTTTCT GCTTCTTACC TACATGGTCA AGCAGTTTGC TGTTTCCTCA 
GGTTCTGAGA AGCTTTTCAG CTCTAAGTCC AATGCCCAAT TCAAAATGTA TAAAACGCCC 
ATTTTCCTGA ATGAAGTCCT GGTGAAACTG CCCACAGACC CTTCCAGCGA TGAGCCTGTC 
TTCCACATTT CTCACATTGA CCGGGTCTAC ACCCTCCGAA CAGACAACAT TAACGAGAGG 
ACTGCTTGGG TCCAGAAGAT CAAGGCGGCA TCTGAGCAGT ACATTGACAC GGAAAAGAAG 
AAACGGGAGA AGGCTTATCA AGCTCGCTCT CAAAAGACTT CAGGCATTGG GCGTCTGATG 
GTGCACGTCA TCGAAGCTAC AGAATTAAAA GCCTGTAAAC CGAATGGAAA GAGCAATCCA 
TACTGTGAAA TCAGCATGGG CTCCCAGAGC TATACCACCA GAACCCTCCA GGACACACTC 
AATCCCAAGT GGAACTTTAA CTGCCAGTTC TTTATTAAGG ACCTTTACCA AGATGTGCTG 
TGTCTCACCA TGTTTGACAG AGACCAGTTT TCACCAGATG ATTTCCTGGG TCGCACTGAA 
GTTCCAGTGG CAAAAATTCG CACAGAACAG GAAAGCAAAG GCCCCACAAC CCGCCGACTC 
CTGCTGCACG AGGTCCCCAC CGGGGAGGTC TGGGTCCGCT TTGACCTGCA GCTTTTTGAG 
CAAAAAACTC TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107825.1
CDS	141..5198
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252057.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252057.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTAAGGGTG AGCCAGCTAC TGCTATGGTG AATTATAGAG CATTGTACCG CTTTGAAGCC 
AGGAGCCATG ATGAGATGAG TTTTAATTCC GGGGATATAA TACAGGTTGA TGAAAAAACC 
GTAGGAGAAC CTGGTTGGCT TTATGGTAGT TTTCAAGGAA ATTTTGGCTG GTTTCCTTGC 
AACTATGTAG AAAAAATGCC ATCAGGTGAA AAATCTGTGT CTCCTAAGAA GGCCTTACTT 
CCCCCTACAG TATCAGTATC TGCTACCTCA ACTTCTTCTG AATCACTTTC TTCAAATCAA 
CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTACAA ACCTAACTGT TAATACATCA 
TGGCAGAAAA AATCAGCTTT TACTCGTACT GTTTCCCCTG GATCTGTATC ACCGATTCAT 
GGCCAGGGAC AGGTTGTAGA AAACCTAAAG GCGCAGGCCC TGTGTTCTTG GACTGCAAAG 
AAGGATAACC ACTTGAACTT TTCAAAACAT GATATTATCA CTGTGTTGGA ACAGCAAGAA 
AATTGGTGGT TTGGAGAGGT GCATGGAGGA AGAGGGTGGT TTCCAAAATC TTATGTCAAA 
ATACTCCCTG GGAGTGAAGT CAAGCGGGAA GAACCAGAAG CTTTGTATGC AGCTGTAAAC 
AAGAAACCTA CCTCTGCAGC CTACACAGGT GGAGAGGAAT ATATTGCACT TTATCCATAT 
TCAAGTGTAG AACCTGGAGA TTTGACTTTC ACTGAAGGTG AAGAAATATT GGTGACCCAG 
AAAGATGGAG AATGGTGGAC AGGAAGTATT GGAGATAGAA CTGGAATTTT TCCATCAAAT 
TATGTCAAAC CAAAAGATCA AGAGAGTTTT GGGAGTGCTA GCAAATCTGG AACATCAAAC 
AAGAAACCTG AGATTGCTCA AGTAACTTCA GCATATGTGG CTTCTGGTTC TGAACAACTT 
AGCCTTGCAC CCGGGCAGTT AATACTAATC CTAAAGAAAA ATTCAAGTGG GTGGTGGCAA 
GGGGAATTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT GGTTTCCTGC CAGCCATGTT 
AAACTTTTGG GTCCAAGTAG TGAAAGAACT ACACCTGCCT TTCATCCTGT ATGTCAGGTG 
ATTGCTATGT ATGACTATGT AGCAAATAAC GAGGATGAGC TGAATTTCTC CAAGGGACAA 
CTTATCAATG TTTTGAACAA AGATGATCCT GACTGGTGGC AAGGAGAAAT CAACGGGGTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCCGATCC AAGTCAACAG 
TGGTGTGCTG ATCTCCAAAG CCTGGACACC ATGCAGCCAG TTGAGAGGAA GAGGCAGGGC 
TATATTCACG AGCTGATTCA GACCGAAGAG CGGTACGTGG ACGACCTTCA GCTCGTCGTG 
GAGGTTTTTC AGAAACGGAT GGTGGAGTCG GGCTTTCTCA GTGAAGGAGA AATGGCCTTG 
ATTTTTGTAA ACTGGAAAGA ACTCATCATG TCTAACACAA AGCTACTCAA GGCCTTGCGG 
GTACGGAAGA AGACCGGGGG AGAGAAGATG CCCGTGCAGA TGATTGGGGA CATCCTGGCA 
GCCGAGCTGT CCCACATGCA GGCTTATATC CGGTTCTGTA GCTGTCAGCT TAATGGAGCA 
GCTCTGTTAC AGCAGAAGAC CGATGAAGAC ACGGATTTCA AGGAATTTTT AAAGAAGCTG 
GCATCTGACC CTCGATGTAA AGGAATGCCC CTTTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGAATCACCC GCTACCCTCT GCTCATCAGA AGTATTCTGG AGAACACCCT GGAGAGTCAT 
GTGGACCATT CCTCCCTAAA GCTGGCTCTT GAGCGGGCAG AGGAGCTTTG TTCCCAAGTT 
AATGAAGGAG TTCGGGAAAA AGAAAATTCA GACCGACTCG AGTGGATCCA GGCACACGTT 
CAGTGTGAAG GTCTTGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGGCCC 
CGGAAGCTTC TGCACAGTGG GAAACTATAC AAGACCAAGA GCAACAAGGA ACTACACGGA 
TTCCTCTTCA ATGACTTTCT GCTTCTTACC TACATGGTCA AGCAGTTTGC TGTTTCCTCA 
GGTTCTGAGA AGCTTTTCAG CTCTAAGTCC AATGCCCAAT TCAAAATGTA TAAAACGCCC 
ATTTTCCTGA ATGAAGTCCT GGTGAAACTG CCCACAGACC CTTCCAGCGA TGAGCCTGTC 
TTCCACATTT CTCACATTGA CCGGGTCTAC ACCCTCCGAA CAGACAACAT TAACGAGAGG 
ACTGCTTGGG TCCAGAAGAT CAAGGCGGCA TCTGAGCAGT ACATTGACAC GGAAAAGAAG 
AAACGGGAGA AGGCTTATCA AGCTCGCTCT CAAAAGACTT CAGGCATTGG GCGTCTGATG 
GTGCACGTCA TCGAAGCTAC AGAATTAAAA GCCTGTAAAC CGAATGGAAA GAGCAATCCA 
TACTGTGAAA TCAGCATGGG CTCCCAGAGC TATACCACCA GAACCCTCCA GGACACACTC 
AATCCCAAGT GGAACTTTAA CTGCCAGTTC TTTATTAAGG ACCTTTACCA AGATGTGCTG 
TGTCTCACCA TGTTTGACAG AGACCAGTTT TCACCAGATG ATTTCCTGGG TCGCACTGAA 
GTTCCAGTGG CAAAAATTCG CACAGAACAG GAAAGCAAAG GCCCCACAAC CCGCCGACTC 
CTGCTGCACG AGGTCCCCAC CGGGGAGGTC TGGGTCCGCT TTGACCTGCA GCTTTTTGAG 
CAAAAAACTC TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252056.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107824.1 Felis catus intersectin 2 (ITSN2), transcript variant X9, mRNA.

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CloneID	OFb73981
Clone ID Related Accession (Same CDS sequence)	XM_023252057.1 , XM_023252056.1 , XM_019827835.2
Accession Version	XM_023252056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTAAGGGTG AGCCAGCTAC TGCTATGGTG AATTATAGAG CATTGTACCG CTTTGAAGCC 
AGGAGCCATG ATGAGATGAG TTTTAATTCC GGGGATATAA TACAGGTTGA TGAAAAAACC 
GTAGGAGAAC CTGGTTGGCT TTATGGTAGT TTTCAAGGAA ATTTTGGCTG GTTTCCTTGC 
AACTATGTAG AAAAAATGCC ATCAGGTGAA AAATCTGTGT CTCCTAAGAA GGCCTTACTT 
CCCCCTACAG TATCAGTATC TGCTACCTCA ACTTCTTCTG AATCACTTTC TTCAAATCAA 
CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTACAA ACCTAACTGT TAATACATCA 
TGGCAGAAAA AATCAGCTTT TACTCGTACT GTTTCCCCTG GATCTGTATC ACCGATTCAT 
GGCCAGGGAC AGGTTGTAGA AAACCTAAAG GCGCAGGCCC TGTGTTCTTG GACTGCAAAG 
AAGGATAACC ACTTGAACTT TTCAAAACAT GATATTATCA CTGTGTTGGA ACAGCAAGAA 
AATTGGTGGT TTGGAGAGGT GCATGGAGGA AGAGGGTGGT TTCCAAAATC TTATGTCAAA 
ATACTCCCTG GGAGTGAAGT CAAGCGGGAA GAACCAGAAG CTTTGTATGC AGCTGTAAAC 
AAGAAACCTA CCTCTGCAGC CTACACAGGT GGAGAGGAAT ATATTGCACT TTATCCATAT 
TCAAGTGTAG AACCTGGAGA TTTGACTTTC ACTGAAGGTG AAGAAATATT GGTGACCCAG 
AAAGATGGAG AATGGTGGAC AGGAAGTATT GGAGATAGAA CTGGAATTTT TCCATCAAAT 
TATGTCAAAC CAAAAGATCA AGAGAGTTTT GGGAGTGCTA GCAAATCTGG AACATCAAAC 
AAGAAACCTG AGATTGCTCA AGTAACTTCA GCATATGTGG CTTCTGGTTC TGAACAACTT 
AGCCTTGCAC CCGGGCAGTT AATACTAATC CTAAAGAAAA ATTCAAGTGG GTGGTGGCAA 
GGGGAATTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT GGTTTCCTGC CAGCCATGTT 
AAACTTTTGG GTCCAAGTAG TGAAAGAACT ACACCTGCCT TTCATCCTGT ATGTCAGGTG 
ATTGCTATGT ATGACTATGT AGCAAATAAC GAGGATGAGC TGAATTTCTC CAAGGGACAA 
CTTATCAATG TTTTGAACAA AGATGATCCT GACTGGTGGC AAGGAGAAAT CAACGGGGTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCCGATCC AAGTCAACAG 
TGGTGTGCTG ATCTCCAAAG CCTGGACACC ATGCAGCCAG TTGAGAGGAA GAGGCAGGGC 
TATATTCACG AGCTGATTCA GACCGAAGAG CGGTACGTGG ACGACCTTCA GCTCGTCGTG 
GAGGTTTTTC AGAAACGGAT GGTGGAGTCG GGCTTTCTCA GTGAAGGAGA AATGGCCTTG 
ATTTTTGTAA ACTGGAAAGA ACTCATCATG TCTAACACAA AGCTACTCAA GGCCTTGCGG 
GTACGGAAGA AGACCGGGGG AGAGAAGATG CCCGTGCAGA TGATTGGGGA CATCCTGGCA 
GCCGAGCTGT CCCACATGCA GGCTTATATC CGGTTCTGTA GCTGTCAGCT TAATGGAGCA 
GCTCTGTTAC AGCAGAAGAC CGATGAAGAC ACGGATTTCA AGGAATTTTT AAAGAAGCTG 
GCATCTGACC CTCGATGTAA AGGAATGCCC CTTTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGAATCACCC GCTACCCTCT GCTCATCAGA AGTATTCTGG AGAACACCCT GGAGAGTCAT 
GTGGACCATT CCTCCCTAAA GCTGGCTCTT GAGCGGGCAG AGGAGCTTTG TTCCCAAGTT 
AATGAAGGAG TTCGGGAAAA AGAAAATTCA GACCGACTCG AGTGGATCCA GGCACACGTT 
CAGTGTGAAG GTCTTGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGGCCC 
CGGAAGCTTC TGCACAGTGG GAAACTATAC AAGACCAAGA GCAACAAGGA ACTACACGGA 
TTCCTCTTCA ATGACTTTCT GCTTCTTACC TACATGGTCA AGCAGTTTGC TGTTTCCTCA 
GGTTCTGAGA AGCTTTTCAG CTCTAAGTCC AATGCCCAAT TCAAAATGTA TAAAACGCCC 
ATTTTCCTGA ATGAAGTCCT GGTGAAACTG CCCACAGACC CTTCCAGCGA TGAGCCTGTC 
TTCCACATTT CTCACATTGA CCGGGTCTAC ACCCTCCGAA CAGACAACAT TAACGAGAGG 
ACTGCTTGGG TCCAGAAGAT CAAGGCGGCA TCTGAGCAGT ACATTGACAC GGAAAAGAAG 
AAACGGGAGA AGGCTTATCA AGCTCGCTCT CAAAAGACTT CAGGCATTGG GCGTCTGATG 
GTGCACGTCA TCGAAGCTAC AGAATTAAAA GCCTGTAAAC CGAATGGAAA GAGCAATCCA 
TACTGTGAAA TCAGCATGGG CTCCCAGAGC TATACCACCA GAACCCTCCA GGACACACTC 
AATCCCAAGT GGAACTTTAA CTGCCAGTTC TTTATTAAGG ACCTTTACCA AGATGTGCTG 
TGTCTCACCA TGTTTGACAG AGACCAGTTT TCACCAGATG ATTTCCTGGG TCGCACTGAA 
GTTCCAGTGG CAAAAATTCG CACAGAACAG GAAAGCAAAG GCCCCACAAC CCGCCGACTC 
CTGCTGCACG AGGTCCCCAC CGGGGAGGTC TGGGTCCGCT TTGACCTGCA GCTTTTTGAG 
CAAAAAACTC TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107824.1
CDS	136..5193
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252056.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252056.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTAAGGGTG AGCCAGCTAC TGCTATGGTG AATTATAGAG CATTGTACCG CTTTGAAGCC 
AGGAGCCATG ATGAGATGAG TTTTAATTCC GGGGATATAA TACAGGTTGA TGAAAAAACC 
GTAGGAGAAC CTGGTTGGCT TTATGGTAGT TTTCAAGGAA ATTTTGGCTG GTTTCCTTGC 
AACTATGTAG AAAAAATGCC ATCAGGTGAA AAATCTGTGT CTCCTAAGAA GGCCTTACTT 
CCCCCTACAG TATCAGTATC TGCTACCTCA ACTTCTTCTG AATCACTTTC TTCAAATCAA 
CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTACAA ACCTAACTGT TAATACATCA 
TGGCAGAAAA AATCAGCTTT TACTCGTACT GTTTCCCCTG GATCTGTATC ACCGATTCAT 
GGCCAGGGAC AGGTTGTAGA AAACCTAAAG GCGCAGGCCC TGTGTTCTTG GACTGCAAAG 
AAGGATAACC ACTTGAACTT TTCAAAACAT GATATTATCA CTGTGTTGGA ACAGCAAGAA 
AATTGGTGGT TTGGAGAGGT GCATGGAGGA AGAGGGTGGT TTCCAAAATC TTATGTCAAA 
ATACTCCCTG GGAGTGAAGT CAAGCGGGAA GAACCAGAAG CTTTGTATGC AGCTGTAAAC 
AAGAAACCTA CCTCTGCAGC CTACACAGGT GGAGAGGAAT ATATTGCACT TTATCCATAT 
TCAAGTGTAG AACCTGGAGA TTTGACTTTC ACTGAAGGTG AAGAAATATT GGTGACCCAG 
AAAGATGGAG AATGGTGGAC AGGAAGTATT GGAGATAGAA CTGGAATTTT TCCATCAAAT 
TATGTCAAAC CAAAAGATCA AGAGAGTTTT GGGAGTGCTA GCAAATCTGG AACATCAAAC 
AAGAAACCTG AGATTGCTCA AGTAACTTCA GCATATGTGG CTTCTGGTTC TGAACAACTT 
AGCCTTGCAC CCGGGCAGTT AATACTAATC CTAAAGAAAA ATTCAAGTGG GTGGTGGCAA 
GGGGAATTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT GGTTTCCTGC CAGCCATGTT 
AAACTTTTGG GTCCAAGTAG TGAAAGAACT ACACCTGCCT TTCATCCTGT ATGTCAGGTG 
ATTGCTATGT ATGACTATGT AGCAAATAAC GAGGATGAGC TGAATTTCTC CAAGGGACAA 
CTTATCAATG TTTTGAACAA AGATGATCCT GACTGGTGGC AAGGAGAAAT CAACGGGGTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCCGATCC AAGTCAACAG 
TGGTGTGCTG ATCTCCAAAG CCTGGACACC ATGCAGCCAG TTGAGAGGAA GAGGCAGGGC 
TATATTCACG AGCTGATTCA GACCGAAGAG CGGTACGTGG ACGACCTTCA GCTCGTCGTG 
GAGGTTTTTC AGAAACGGAT GGTGGAGTCG GGCTTTCTCA GTGAAGGAGA AATGGCCTTG 
ATTTTTGTAA ACTGGAAAGA ACTCATCATG TCTAACACAA AGCTACTCAA GGCCTTGCGG 
GTACGGAAGA AGACCGGGGG AGAGAAGATG CCCGTGCAGA TGATTGGGGA CATCCTGGCA 
GCCGAGCTGT CCCACATGCA GGCTTATATC CGGTTCTGTA GCTGTCAGCT TAATGGAGCA 
GCTCTGTTAC AGCAGAAGAC CGATGAAGAC ACGGATTTCA AGGAATTTTT AAAGAAGCTG 
GCATCTGACC CTCGATGTAA AGGAATGCCC CTTTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGAATCACCC GCTACCCTCT GCTCATCAGA AGTATTCTGG AGAACACCCT GGAGAGTCAT 
GTGGACCATT CCTCCCTAAA GCTGGCTCTT GAGCGGGCAG AGGAGCTTTG TTCCCAAGTT 
AATGAAGGAG TTCGGGAAAA AGAAAATTCA GACCGACTCG AGTGGATCCA GGCACACGTT 
CAGTGTGAAG GTCTTGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGGCCC 
CGGAAGCTTC TGCACAGTGG GAAACTATAC AAGACCAAGA GCAACAAGGA ACTACACGGA 
TTCCTCTTCA ATGACTTTCT GCTTCTTACC TACATGGTCA AGCAGTTTGC TGTTTCCTCA 
GGTTCTGAGA AGCTTTTCAG CTCTAAGTCC AATGCCCAAT TCAAAATGTA TAAAACGCCC 
ATTTTCCTGA ATGAAGTCCT GGTGAAACTG CCCACAGACC CTTCCAGCGA TGAGCCTGTC 
TTCCACATTT CTCACATTGA CCGGGTCTAC ACCCTCCGAA CAGACAACAT TAACGAGAGG 
ACTGCTTGGG TCCAGAAGAT CAAGGCGGCA TCTGAGCAGT ACATTGACAC GGAAAAGAAG 
AAACGGGAGA AGGCTTATCA AGCTCGCTCT CAAAAGACTT CAGGCATTGG GCGTCTGATG 
GTGCACGTCA TCGAAGCTAC AGAATTAAAA GCCTGTAAAC CGAATGGAAA GAGCAATCCA 
TACTGTGAAA TCAGCATGGG CTCCCAGAGC TATACCACCA GAACCCTCCA GGACACACTC 
AATCCCAAGT GGAACTTTAA CTGCCAGTTC TTTATTAAGG ACCTTTACCA AGATGTGCTG 
TGTCTCACCA TGTTTGACAG AGACCAGTTT TCACCAGATG ATTTCCTGGG TCGCACTGAA 
GTTCCAGTGG CAAAAATTCG CACAGAACAG GAAAGCAAAG GCCCCACAAC CCGCCGACTC 
CTGCTGCACG AGGTCCCCAC CGGGGAGGTC TGGGTCCGCT TTGACCTGCA GCTTTTTGAG 
CAAAAAACTC TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827835.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683394.2 Felis catus intersectin 2 (ITSN2), transcript variant X11, mRNA.

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CloneID	OFb73981
Clone ID Related Accession (Same CDS sequence)	XM_023252057.1 , XM_023252056.1 , XM_019827835.2
Accession Version	XM_019827835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019827835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTAAGGGTG AGCCAGCTAC TGCTATGGTG AATTATAGAG CATTGTACCG CTTTGAAGCC 
AGGAGCCATG ATGAGATGAG TTTTAATTCC GGGGATATAA TACAGGTTGA TGAAAAAACC 
GTAGGAGAAC CTGGTTGGCT TTATGGTAGT TTTCAAGGAA ATTTTGGCTG GTTTCCTTGC 
AACTATGTAG AAAAAATGCC ATCAGGTGAA AAATCTGTGT CTCCTAAGAA GGCCTTACTT 
CCCCCTACAG TATCAGTATC TGCTACCTCA ACTTCTTCTG AATCACTTTC TTCAAATCAA 
CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTACAA ACCTAACTGT TAATACATCA 
TGGCAGAAAA AATCAGCTTT TACTCGTACT GTTTCCCCTG GATCTGTATC ACCGATTCAT 
GGCCAGGGAC AGGTTGTAGA AAACCTAAAG GCGCAGGCCC TGTGTTCTTG GACTGCAAAG 
AAGGATAACC ACTTGAACTT TTCAAAACAT GATATTATCA CTGTGTTGGA ACAGCAAGAA 
AATTGGTGGT TTGGAGAGGT GCATGGAGGA AGAGGGTGGT TTCCAAAATC TTATGTCAAA 
ATACTCCCTG GGAGTGAAGT CAAGCGGGAA GAACCAGAAG CTTTGTATGC AGCTGTAAAC 
AAGAAACCTA CCTCTGCAGC CTACACAGGT GGAGAGGAAT ATATTGCACT TTATCCATAT 
TCAAGTGTAG AACCTGGAGA TTTGACTTTC ACTGAAGGTG AAGAAATATT GGTGACCCAG 
AAAGATGGAG AATGGTGGAC AGGAAGTATT GGAGATAGAA CTGGAATTTT TCCATCAAAT 
TATGTCAAAC CAAAAGATCA AGAGAGTTTT GGGAGTGCTA GCAAATCTGG AACATCAAAC 
AAGAAACCTG AGATTGCTCA AGTAACTTCA GCATATGTGG CTTCTGGTTC TGAACAACTT 
AGCCTTGCAC CCGGGCAGTT AATACTAATC CTAAAGAAAA ATTCAAGTGG GTGGTGGCAA 
GGGGAATTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT GGTTTCCTGC CAGCCATGTT 
AAACTTTTGG GTCCAAGTAG TGAAAGAACT ACACCTGCCT TTCATCCTGT ATGTCAGGTG 
ATTGCTATGT ATGACTATGT AGCAAATAAC GAGGATGAGC TGAATTTCTC CAAGGGACAA 
CTTATCAATG TTTTGAACAA AGATGATCCT GACTGGTGGC AAGGAGAAAT CAACGGGGTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCCGATCC AAGTCAACAG 
TGGTGTGCTG ATCTCCAAAG CCTGGACACC ATGCAGCCAG TTGAGAGGAA GAGGCAGGGC 
TATATTCACG AGCTGATTCA GACCGAAGAG CGGTACGTGG ACGACCTTCA GCTCGTCGTG 
GAGGTTTTTC AGAAACGGAT GGTGGAGTCG GGCTTTCTCA GTGAAGGAGA AATGGCCTTG 
ATTTTTGTAA ACTGGAAAGA ACTCATCATG TCTAACACAA AGCTACTCAA GGCCTTGCGG 
GTACGGAAGA AGACCGGGGG AGAGAAGATG CCCGTGCAGA TGATTGGGGA CATCCTGGCA 
GCCGAGCTGT CCCACATGCA GGCTTATATC CGGTTCTGTA GCTGTCAGCT TAATGGAGCA 
GCTCTGTTAC AGCAGAAGAC CGATGAAGAC ACGGATTTCA AGGAATTTTT AAAGAAGCTG 
GCATCTGACC CTCGATGTAA AGGAATGCCC CTTTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGAATCACCC GCTACCCTCT GCTCATCAGA AGTATTCTGG AGAACACCCT GGAGAGTCAT 
GTGGACCATT CCTCCCTAAA GCTGGCTCTT GAGCGGGCAG AGGAGCTTTG TTCCCAAGTT 
AATGAAGGAG TTCGGGAAAA AGAAAATTCA GACCGACTCG AGTGGATCCA GGCACACGTT 
CAGTGTGAAG GTCTTGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGGCCC 
CGGAAGCTTC TGCACAGTGG GAAACTATAC AAGACCAAGA GCAACAAGGA ACTACACGGA 
TTCCTCTTCA ATGACTTTCT GCTTCTTACC TACATGGTCA AGCAGTTTGC TGTTTCCTCA 
GGTTCTGAGA AGCTTTTCAG CTCTAAGTCC AATGCCCAAT TCAAAATGTA TAAAACGCCC 
ATTTTCCTGA ATGAAGTCCT GGTGAAACTG CCCACAGACC CTTCCAGCGA TGAGCCTGTC 
TTCCACATTT CTCACATTGA CCGGGTCTAC ACCCTCCGAA CAGACAACAT TAACGAGAGG 
ACTGCTTGGG TCCAGAAGAT CAAGGCGGCA TCTGAGCAGT ACATTGACAC GGAAAAGAAG 
AAACGGGAGA AGGCTTATCA AGCTCGCTCT CAAAAGACTT CAGGCATTGG GCGTCTGATG 
GTGCACGTCA TCGAAGCTAC AGAATTAAAA GCCTGTAAAC CGAATGGAAA GAGCAATCCA 
TACTGTGAAA TCAGCATGGG CTCCCAGAGC TATACCACCA GAACCCTCCA GGACACACTC 
AATCCCAAGT GGAACTTTAA CTGCCAGTTC TTTATTAAGG ACCTTTACCA AGATGTGCTG 
TGTCTCACCA TGTTTGACAG AGACCAGTTT TCACCAGATG ATTTCCTGGG TCGCACTGAA 
GTTCCAGTGG CAAAAATTCG CACAGAACAG GAAAGCAAAG GCCCCACAAC CCGCCGACTC 
CTGCTGCACG AGGTCCCCAC CGGGGAGGTC TGGGTCCGCT TTGACCTGCA GCTTTTTGAG 
CAAAAAACTC TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683394.2
CDS	160..5217
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827835.2
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827835.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTAAGGGTG AGCCAGCTAC TGCTATGGTG AATTATAGAG CATTGTACCG CTTTGAAGCC 
AGGAGCCATG ATGAGATGAG TTTTAATTCC GGGGATATAA TACAGGTTGA TGAAAAAACC 
GTAGGAGAAC CTGGTTGGCT TTATGGTAGT TTTCAAGGAA ATTTTGGCTG GTTTCCTTGC 
AACTATGTAG AAAAAATGCC ATCAGGTGAA AAATCTGTGT CTCCTAAGAA GGCCTTACTT 
CCCCCTACAG TATCAGTATC TGCTACCTCA ACTTCTTCTG AATCACTTTC TTCAAATCAA 
CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTACAA ACCTAACTGT TAATACATCA 
TGGCAGAAAA AATCAGCTTT TACTCGTACT GTTTCCCCTG GATCTGTATC ACCGATTCAT 
GGCCAGGGAC AGGTTGTAGA AAACCTAAAG GCGCAGGCCC TGTGTTCTTG GACTGCAAAG 
AAGGATAACC ACTTGAACTT TTCAAAACAT GATATTATCA CTGTGTTGGA ACAGCAAGAA 
AATTGGTGGT TTGGAGAGGT GCATGGAGGA AGAGGGTGGT TTCCAAAATC TTATGTCAAA 
ATACTCCCTG GGAGTGAAGT CAAGCGGGAA GAACCAGAAG CTTTGTATGC AGCTGTAAAC 
AAGAAACCTA CCTCTGCAGC CTACACAGGT GGAGAGGAAT ATATTGCACT TTATCCATAT 
TCAAGTGTAG AACCTGGAGA TTTGACTTTC ACTGAAGGTG AAGAAATATT GGTGACCCAG 
AAAGATGGAG AATGGTGGAC AGGAAGTATT GGAGATAGAA CTGGAATTTT TCCATCAAAT 
TATGTCAAAC CAAAAGATCA AGAGAGTTTT GGGAGTGCTA GCAAATCTGG AACATCAAAC 
AAGAAACCTG AGATTGCTCA AGTAACTTCA GCATATGTGG CTTCTGGTTC TGAACAACTT 
AGCCTTGCAC CCGGGCAGTT AATACTAATC CTAAAGAAAA ATTCAAGTGG GTGGTGGCAA 
GGGGAATTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT GGTTTCCTGC CAGCCATGTT 
AAACTTTTGG GTCCAAGTAG TGAAAGAACT ACACCTGCCT TTCATCCTGT ATGTCAGGTG 
ATTGCTATGT ATGACTATGT AGCAAATAAC GAGGATGAGC TGAATTTCTC CAAGGGACAA 
CTTATCAATG TTTTGAACAA AGATGATCCT GACTGGTGGC AAGGAGAAAT CAACGGGGTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCCGATCC AAGTCAACAG 
TGGTGTGCTG ATCTCCAAAG CCTGGACACC ATGCAGCCAG TTGAGAGGAA GAGGCAGGGC 
TATATTCACG AGCTGATTCA GACCGAAGAG CGGTACGTGG ACGACCTTCA GCTCGTCGTG 
GAGGTTTTTC AGAAACGGAT GGTGGAGTCG GGCTTTCTCA GTGAAGGAGA AATGGCCTTG 
ATTTTTGTAA ACTGGAAAGA ACTCATCATG TCTAACACAA AGCTACTCAA GGCCTTGCGG 
GTACGGAAGA AGACCGGGGG AGAGAAGATG CCCGTGCAGA TGATTGGGGA CATCCTGGCA 
GCCGAGCTGT CCCACATGCA GGCTTATATC CGGTTCTGTA GCTGTCAGCT TAATGGAGCA 
GCTCTGTTAC AGCAGAAGAC CGATGAAGAC ACGGATTTCA AGGAATTTTT AAAGAAGCTG 
GCATCTGACC CTCGATGTAA AGGAATGCCC CTTTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGAATCACCC GCTACCCTCT GCTCATCAGA AGTATTCTGG AGAACACCCT GGAGAGTCAT 
GTGGACCATT CCTCCCTAAA GCTGGCTCTT GAGCGGGCAG AGGAGCTTTG TTCCCAAGTT 
AATGAAGGAG TTCGGGAAAA AGAAAATTCA GACCGACTCG AGTGGATCCA GGCACACGTT 
CAGTGTGAAG GTCTTGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGGCCC 
CGGAAGCTTC TGCACAGTGG GAAACTATAC AAGACCAAGA GCAACAAGGA ACTACACGGA 
TTCCTCTTCA ATGACTTTCT GCTTCTTACC TACATGGTCA AGCAGTTTGC TGTTTCCTCA 
GGTTCTGAGA AGCTTTTCAG CTCTAAGTCC AATGCCCAAT TCAAAATGTA TAAAACGCCC 
ATTTTCCTGA ATGAAGTCCT GGTGAAACTG CCCACAGACC CTTCCAGCGA TGAGCCTGTC 
TTCCACATTT CTCACATTGA CCGGGTCTAC ACCCTCCGAA CAGACAACAT TAACGAGAGG 
ACTGCTTGGG TCCAGAAGAT CAAGGCGGCA TCTGAGCAGT ACATTGACAC GGAAAAGAAG 
AAACGGGAGA AGGCTTATCA AGCTCGCTCT CAAAAGACTT CAGGCATTGG GCGTCTGATG 
GTGCACGTCA TCGAAGCTAC AGAATTAAAA GCCTGTAAAC CGAATGGAAA GAGCAATCCA 
TACTGTGAAA TCAGCATGGG CTCCCAGAGC TATACCACCA GAACCCTCCA GGACACACTC 
AATCCCAAGT GGAACTTTAA CTGCCAGTTC TTTATTAAGG ACCTTTACCA AGATGTGCTG 
TGTCTCACCA TGTTTGACAG AGACCAGTTT TCACCAGATG ATTTCCTGGG TCGCACTGAA 
GTTCCAGTGG CAAAAATTCG CACAGAACAG GAAAGCAAAG GCCCCACAAC CCGCCGACTC 
CTGCTGCACG AGGTCCCCAC CGGGGAGGTC TGGGTCCGCT TTGACCTGCA GCTTTTTGAG 
CAAAAAACTC TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252058.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107826.1 Felis catus intersectin 2 (ITSN2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OFb73982
Clone ID Related Accession (Same CDS sequence)	XM_023252058.1
Accession Version	XM_023252058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTGAGCCAG CTACTGCTAT GGTGAATTAT AGAGCATTGT ACCGCTTTGA AGCCAGGAGC 
CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCGTAGGA 
GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT 
GTAGAAAAAA TGCCATCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT 
ACAGTATCAG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG 
AAAAAATCAG CTTTTACTCG TACTGTTTCC CCTGGATCTG TATCACCGAT TCATGGCCAG 
GGACAGGTTG TAGAAAACCT AAAGGCGCAG GCCCTGTGTT CTTGGACTGC AAAGAAGGAT 
AACCACTTGA ACTTTTCAAA ACATGATATT ATCACTGTGT TGGAACAGCA AGAAAATTGG 
TGGTTTGGAG AGGTGCATGG AGGAAGAGGG TGGTTTCCAA AATCTTATGT CAAAATACTC 
CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAACAAGAAA 
CCTACCTCTG CAGCCTACAC AGGTGGAGAG GAATATATTG CACTTTATCC ATATTCAAGT 
GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAATGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC 
AAACCAAAAG ATCAAGAGAG TTTTGGGAGT GCTAGCAAAT CTGGAACATC AAACAAGAAA 
CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTGGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCCGGGC AGTTAATACT AATCCTAAAG AAAAATTCAA GTGGGTGGTG GCAAGGGGAA 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AACTACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGTAGCAAA TAACGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC 
AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCCG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTCC AAAGCCTGGA CACCATGCAG CCAGTTGAGA GGAAGAGGCA GGGCTATATT 
CACGAGCTGA TTCAGACCGA AGAGCGGTAC GTGGACGACC TTCAGCTCGT CGTGGAGGTT 
TTTCAGAAAC GGATGGTGGA GTCGGGCTTT CTCAGTGAAG GAGAAATGGC CTTGATTTTT 
GTAAACTGGA AAGAACTCAT CATGTCTAAC ACAAAGCTAC TCAAGGCCTT GCGGGTACGG 
AAGAAGACCG GGGGAGAGAA GATGCCCGTG CAGATGATTG GGGACATCCT GGCAGCCGAG 
CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACCGATGA AGACACGGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT 
GACCCTCGAT GTAAAGGAAT GCCCCTTTCC AGCTTCCTGC TGAAGCCCAT GCAGAGAATC 
ACCCGCTACC CTCTGCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAG TCATGTGGAC 
CATTCCTCCC TAAAGCTGGC TCTTGAGCGG GCAGAGGAGC TTTGTTCCCA AGTTAATGAA 
GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTCGAGTGGA TCCAGGCACA CGTTCAGTGT 
GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCCCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTTCTGCACA GTGGGAAACT ATACAAGACC AAGAGCAACA AGGAACTACA CGGATTCCTC 
TTCAATGACT TTCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCAGGTTCT 
GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCCTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCTCACA TTGACCGGGT CTACACCCTC CGAACAGACA ACATTAACGA GAGGACTGCT 
TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATTG ACACGGAAAA GAAGAAACGG 
GAGAAGGCTT ATCAAGCTCG CTCTCAAAAG ACTTCAGGCA TTGGGCGTCT GATGGTGCAC 
GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCGAATG GAAAGAGCAA TCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCAGGACAC ACTCAATCCC 
AAGTGGAACT TTAACTGCCA GTTCTTTATT AAGGACCTTT ACCAAGATGT GCTGTGTCTC 
ACCATGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGCAC TGAAGTTCCA 
GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTCCTGCTG 
CACGAGGTCC CCACCGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107826.1
CDS	136..5187
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252058.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252058.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTGAGCCAG CTACTGCTAT GGTGAATTAT AGAGCATTGT ACCGCTTTGA AGCCAGGAGC 
CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCGTAGGA 
GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT 
GTAGAAAAAA TGCCATCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT 
ACAGTATCAG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG 
AAAAAATCAG CTTTTACTCG TACTGTTTCC CCTGGATCTG TATCACCGAT TCATGGCCAG 
GGACAGGTTG TAGAAAACCT AAAGGCGCAG GCCCTGTGTT CTTGGACTGC AAAGAAGGAT 
AACCACTTGA ACTTTTCAAA ACATGATATT ATCACTGTGT TGGAACAGCA AGAAAATTGG 
TGGTTTGGAG AGGTGCATGG AGGAAGAGGG TGGTTTCCAA AATCTTATGT CAAAATACTC 
CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAACAAGAAA 
CCTACCTCTG CAGCCTACAC AGGTGGAGAG GAATATATTG CACTTTATCC ATATTCAAGT 
GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAATGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC 
AAACCAAAAG ATCAAGAGAG TTTTGGGAGT GCTAGCAAAT CTGGAACATC AAACAAGAAA 
CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTGGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCCGGGC AGTTAATACT AATCCTAAAG AAAAATTCAA GTGGGTGGTG GCAAGGGGAA 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AACTACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGTAGCAAA TAACGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC 
AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCCG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTCC AAAGCCTGGA CACCATGCAG CCAGTTGAGA GGAAGAGGCA GGGCTATATT 
CACGAGCTGA TTCAGACCGA AGAGCGGTAC GTGGACGACC TTCAGCTCGT CGTGGAGGTT 
TTTCAGAAAC GGATGGTGGA GTCGGGCTTT CTCAGTGAAG GAGAAATGGC CTTGATTTTT 
GTAAACTGGA AAGAACTCAT CATGTCTAAC ACAAAGCTAC TCAAGGCCTT GCGGGTACGG 
AAGAAGACCG GGGGAGAGAA GATGCCCGTG CAGATGATTG GGGACATCCT GGCAGCCGAG 
CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACCGATGA AGACACGGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT 
GACCCTCGAT GTAAAGGAAT GCCCCTTTCC AGCTTCCTGC TGAAGCCCAT GCAGAGAATC 
ACCCGCTACC CTCTGCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAG TCATGTGGAC 
CATTCCTCCC TAAAGCTGGC TCTTGAGCGG GCAGAGGAGC TTTGTTCCCA AGTTAATGAA 
GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTCGAGTGGA TCCAGGCACA CGTTCAGTGT 
GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCCCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTTCTGCACA GTGGGAAACT ATACAAGACC AAGAGCAACA AGGAACTACA CGGATTCCTC 
TTCAATGACT TTCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCAGGTTCT 
GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCCTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCTCACA TTGACCGGGT CTACACCCTC CGAACAGACA ACATTAACGA GAGGACTGCT 
TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATTG ACACGGAAAA GAAGAAACGG 
GAGAAGGCTT ATCAAGCTCG CTCTCAAAAG ACTTCAGGCA TTGGGCGTCT GATGGTGCAC 
GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCGAATG GAAAGAGCAA TCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCAGGACAC ACTCAATCCC 
AAGTGGAACT TTAACTGCCA GTTCTTTATT AAGGACCTTT ACCAAGATGT GCTGTGTCTC 
ACCATGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGCAC TGAAGTTCCA 
GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTCCTGCTG 
CACGAGGTCC CCACCGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827838.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683397.2 Felis catus intersectin 2 (ITSN2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OFb73983
Clone ID Related Accession (Same CDS sequence)	XM_019827838.2
Accession Version	XM_019827838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019827838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTA TTACCTCTGA AGAACGGACT AAGCATGACA AGCAGTTTGA TAACCTCAAA 
CCCTCAGGAG GTTACATAAC AGGTGATCAA GCACGTACCT TTTTCTTACA GTCAGGCCTG 
CCGGCTCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG 
GATCAGCAAG AATTCTCCAT AGCGATGAAG CTCATCAAAC TGAAGCTACA AGGCCAGCAG 
CTGCCCGTGG TCCTCCCTCC TATTATGAAA CAACCTCCTA TGTTCTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCACTCATT GCCTCCAGTT 
GCACCTATGG CAACCCCCTT ACCCTCTGCG ACTTCAGGGA CCACCCTTCC TCCCTTAATG 
ATGCCTGCTC CCCTAGTGCC TTCTGTTAGC ACATCGTCAC TACCAAATGG AACCGCTGGC 
CTCATTCAGC CGTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC 
AGTCTCATGA TGGGAGGACT CGGCGGTGCT AGTTTACAGA AAGCCCAGTC TCTGATTGAT 
TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCACTCT CTGGAAACTC GCCCAAGACT 
GGGACCTTAG AGTGGGCGGT TCCTCAGCCT TCAAGGTTAA AATATCGGCA AAAATTTAAT 
AGTCTTGACA AAAGCATGAG TGGATATCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT 
CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATTTGGA CCCTTGCTGA CATTGATGGC 
GACGGACAGC TGAAAGCTGA AGAGTTCATC CTTGCGATGC ATCTCACTGA CATGGCCAAA 
GCTGGACAGC CCTTGCCACT GACGTTGCCT CCTGAACTTG TCCCTCCATC TTTCAGAGGA 
GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCCT ATCAGAAAAT TCAAGAAGAG 
GAACCTCAGA AAAAATTACC AGTTACTTTC GAAGACAAGC GGAAGGCCAA CTACGAGCGA 
GGGAACGTGG AACTGGAGAA GCGGCGCCAA GCCCTGATGG AGCAGCAGCA GAGGGAGGCA 
GAGCGAAAAG CCCAGAAAGA AAAGGAAGAG TGGGAGCGAA AACAGAGAGA ATTACAGGAA 
CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCTTGG AGAAGCAGAG GGAATTGGAG 
AGACAGCGGG AGGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAA 
CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGGATTCGTC GCCAGGAGCT TCTCAATCAA 
AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG TCTTCATCTC 
GAGTTGGAAG CACTGAATGG CAAACACCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA 
CTCCGAAAAC AAACTCAAAA GACTGAGCTG GAAGTTCTAG ATAAACAGTG TGACCTGGAA 
ATTATGGAAA TCAAGCAACT CCAACAAGAA CTTCAGGATT ATCAAAATAA ACTTATCTAT 
TTGGTACCGG AGAAGCAATT ATTAAATGAA AGAATTAAGA ACATGCAGCT TGGTAACACA 
CCTGATTTAG GGATTGGTTT GCTTCATAAA AAATCATTGG AAAAGGAAGA ATTATGCCAA 
AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT ATCTGAAATG 
GATTCATTTA ACAATCAATT AAAGTGTGGG AATATGGATG ACTCTGTTCT GCAGTGCCTT 
TTGTCTCTGC TAAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT GAGAGAAAGC 
TACAATACAC AGCAGTTAGC CCTTGAACAA CTTTATAAGA TCAAACGTGA CAAGTTGAAG 
GAAATTGAAA GAAAAAGGTC AGAGCTAATA CAGAAGAAGA AACTAGAAGA TGAGGCTGTA 
AGGAAAGCAA AGCAAGGAAA AGAAAACTTA TGGAGAGAAA ACCTTAGAAA AGAGGAAGAA 
GAAAAACAAA AGCGACTCCA GGAAGAAAAA ACACAAGAAA AAGTTCAAGA AGAGGAACGG 
AAGGCTGAGG AGAAACCATG TAAGGGTGAG CCAGCTACTG CTATGGTGAA TTATAGAGCA 
TTGTACCGCT TTGAAGCCAG GAGCCATGAT GAGATGAGTT TTAATTCCGG GGATATAATA 
CAGGTTGATG AAAAAACCGT AGGAGAACCT GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT 
TTTGGCTGGT TTCCTTGCAA CTATGTAGAA AAAATGCCAT CAGGTGAAAA ATCTGTGTCT 
CCTAAGAAGG CCTTACTTCC CCCTACAGTA TCAGTATCTG CTACCTCAAC TTCTTCTGAA 
TCACTTTCTT CAAATCAACC AGCATCAGTG ACTGATTATC AAAATGTATC TTTTACAAAC 
CTAACTGTTA ATACATCATG GCAGAAAAAA TCAGCTTTTA CTCGTACTGT TTCCCCTGGA 
TCTGTATCAC CGATTCATGG CCAGGGACAG GTTGTAGAAA ACCTAAAGGC GCAGGCCCTG 
TGTTCTTGGA CTGCAAAGAA GGATAACCAC TTGAACTTTT CAAAACATGA TATTATCACT 
GTGTTGGAAC AGCAAGAAAA TTGGTGGTTT GGAGAGGTGC ATGGAGGAAG AGGGTGGTTT 
CCAAAATCTT ATGTCAAAAT ACTCCCTGGG AGTGAAGTCA AGCGGGAAGA ACCAGAAGCT 
TTGTATGCAG CTGTAAACAA GAAACCTACC TCTGCAGCCT ACACAGGTGG AGAGGAATAT 
ATTGCACTTT ATCCATATTC AAGTGTAGAA CCTGGAGATT TGACTTTCAC TGAAGGTGAA 
GAAATATTGG TGACCCAGAA AGATGGAGAA TGGTGGACAG GAAGTATTGG AGATAGAACT 
GGAATTTTTC CATCAAATTA TGTCAAACCA AAAGATCAAG AGAGTTTTGG GAGTGCTAGC 
AAATCTGGAA CATCAAACAA GAAACCTGAG ATTGCTCAAG TAACTTCAGC ATATGTGGCT 
TCTGGTTCTG AACAACTTAG CCTTGCACCC GGGCAGTTAA TACTAATCCT AAAGAAAAAT 
TCAAGTGGGT GGTGGCAAGG GGAATTACAG GCCAGAGGAA AAAAGCGACA GAAAGGATGG 
TTTCCTGCCA GCCATGTTAA ACTTTTGGGT CCAAGTAGTG AAAGAACTAC ACCTGCCTTT 
CATCCTGTAT GTCAGGTGAT TGCTATGTAT GACTATGTAG CAAATAACGA GGATGAGCTG 
AATTTCTCCA AGGGACAACT TATCAATGTT TTGAACAAAG ATGATCCTGA CTGGTGGCAA 
GGAGAAATCA ACGGGGTGAC TGGTCTCTTT CCTTCAAACT ATGTTAAGAT GACAACAGAC 
TCCGATCCAA GTCAACAGTG GTGTGCTGAT CTCCAAAGCC TGGACACCAT GCAGCCAGTT 
GAGAGGAAGA GGCAGGGCTA TATTCACGAG CTGATTCAGA CCGAAGAGCG GTACGTGGAC 
GACCTTCAGC TCGTCGTGGA GGTTTTTCAG AAACGGATGG TGGAGTCGGG CTTTCTCAGT 
GAAGGAGAAA TGGCCTTGAT TTTTGTAAAC TGGAAAGAAC TCATCATGTC TAACACAAAG 
CTACTCAAGG CCTTGCGGGT ACGGAAGAAG ACCGGGGGAG AGAAGATGCC CGTGCAGATG 
ATTGGGGACA TCCTGGCAGC CGAGCTGTCC CACATGCAGG CTTATATCCG GTTCTGTAGC 
TGTCAGCTTA ATGGAGCAGC TCTGTTACAG CAGAAGACCG ATGAAGACAC GGATTTCAAG 
GAATTTTTAA AGAAGCTGGC ATCTGACCCT CGATGTAAAG GAATGCCCCT TTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG AATCACCCGC TACCCTCTGC TCATCAGAAG TATTCTGGAG 
AACACCCTGG AGAGTCATGT GGACCATTCC TCCCTAAAGC TGGCTCTTGA GCGGGCAGAG 
GAGCTTTGTT CCCAAGTTAA TGAAGGAGTT CGGGAAAAAG AAAATTCAGA CCGACTCGAG 
TGGATCCAGG CACACGTTCA GTGTGAAGGT CTTGCAGAGC AACTTATTTT CAACTCCCTC 
ACCAACTGCC TGGGGCCCCG GAAGCTTCTG CACAGTGGGA AACTATACAA GACCAAGAGC 
AACAAGGAAC TACACGGATT CCTCTTCAAT GACTTTCTGC TTCTTACCTA CATGGTCAAG 
CAGTTTGCTG TTTCCTCAGG TTCTGAGAAG CTTTTCAGCT CTAAGTCCAA TGCCCAATTC 
AAAATGTATA AAACGCCCAT TTTCCTGAAT GAAGTCCTGG TGAAACTGCC CACAGACCCT 
TCCAGCGATG AGCCTGTCTT CCACATTTCT CACATTGACC GGGTCTACAC CCTCCGAACA 
GACAACATTA ACGAGAGGAC TGCTTGGGTC CAGAAGATCA AGGCGGCATC TGAGCAGTAC 
ATTGACACGG AAAAGAAGAA ACGGGAGAAG GCTTATCAAG CTCGCTCTCA AAAGACTTCA 
GGCATTGGGC GTCTGATGGT GCACGTCATC GAAGCTACAG AATTAAAAGC CTGTAAACCG 
AATGGAAAGA GCAATCCATA CTGTGAAATC AGCATGGGCT CCCAGAGCTA TACCACCAGA 
ACCCTCCAGG ACACACTCAA TCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAC 
CTTTACCAAG ATGTGCTGTG TCTCACCATG TTTGACAGAG ACCAGTTTTC ACCAGATGAT 
TTCCTGGGTC GCACTGAAGT TCCAGTGGCA AAAATTCGCA CAGAACAGGA AAGCAAAGGC 
CCCACAACCC GCCGACTCCT GCTGCACGAG GTCCCCACCG GGGAGGTCTG GGTCCGCTTT 
GACCTGCAGC TTTTTGAGCA AAAAACTCTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683397.2
CDS	108..5123
Translation	MWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827838.2
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827838.2

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGGACT AAGCATGACA AGCAGTTTGA TAACCTCAAA 
CCCTCAGGAG GTTACATAAC AGGTGATCAA GCACGTACCT TTTTCTTACA GTCAGGCCTG 
CCGGCTCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG 
GATCAGCAAG AATTCTCCAT AGCGATGAAG CTCATCAAAC TGAAGCTACA AGGCCAGCAG 
CTGCCCGTGG TCCTCCCTCC TATTATGAAA CAACCTCCTA TGTTCTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCACTCATT GCCTCCAGTT 
GCACCTATGG CAACCCCCTT ACCCTCTGCG ACTTCAGGGA CCACCCTTCC TCCCTTAATG 
ATGCCTGCTC CCCTAGTGCC TTCTGTTAGC ACATCGTCAC TACCAAATGG AACCGCTGGC 
CTCATTCAGC CGTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATAC GTCATCTTAC 
AGTCTCATGA TGGGAGGACT CGGCGGTGCT AGTTTACAGA AAGCCCAGTC TCTGATTGAT 
TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCACTCT CTGGAAACTC GCCCAAGACT 
GGGACCTTAG AGTGGGCGGT TCCTCAGCCT TCAAGGTTAA AATATCGGCA AAAATTTAAT 
AGTCTTGACA AAAGCATGAG TGGATATCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT 
CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATTTGGA CCCTTGCTGA CATTGATGGC 
GACGGACAGC TGAAAGCTGA AGAGTTCATC CTTGCGATGC ATCTCACTGA CATGGCCAAA 
GCTGGACAGC CCTTGCCACT GACGTTGCCT CCTGAACTTG TCCCTCCATC TTTCAGAGGA 
GGAAAGCAAA TTGATTCCAT TAATGGAACT CTGCCTTCCT ATCAGAAAAT TCAAGAAGAG 
GAACCTCAGA AAAAATTACC AGTTACTTTC GAAGACAAGC GGAAGGCCAA CTACGAGCGA 
GGGAACGTGG AACTGGAGAA GCGGCGCCAA GCCCTGATGG AGCAGCAGCA GAGGGAGGCA 
GAGCGAAAAG CCCAGAAAGA AAAGGAAGAG TGGGAGCGAA AACAGAGAGA ATTACAGGAA 
CAAGAATGGA AGAAACAACT TGAATTAGAA AAACGCTTGG AGAAGCAGAG GGAATTGGAG 
AGACAGCGGG AGGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAA 
CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGGATTCGTC GCCAGGAGCT TCTCAATCAA 
AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG TCTTCATCTC 
GAGTTGGAAG CACTGAATGG CAAACACCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA 
CTCCGAAAAC AAACTCAAAA GACTGAGCTG GAAGTTCTAG ATAAACAGTG TGACCTGGAA 
ATTATGGAAA TCAAGCAACT CCAACAAGAA CTTCAGGATT ATCAAAATAA ACTTATCTAT 
TTGGTACCGG AGAAGCAATT ATTAAATGAA AGAATTAAGA ACATGCAGCT TGGTAACACA 
CCTGATTTAG GGATTGGTTT GCTTCATAAA AAATCATTGG AAAAGGAAGA ATTATGCCAA 
AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT ATCTGAAATG 
GATTCATTTA ACAATCAATT AAAGTGTGGG AATATGGATG ACTCTGTTCT GCAGTGCCTT 
TTGTCTCTGC TAAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT GAGAGAAAGC 
TACAATACAC AGCAGTTAGC CCTTGAACAA CTTTATAAGA TCAAACGTGA CAAGTTGAAG 
GAAATTGAAA GAAAAAGGTC AGAGCTAATA CAGAAGAAGA AACTAGAAGA TGAGGCTGTA 
AGGAAAGCAA AGCAAGGAAA AGAAAACTTA TGGAGAGAAA ACCTTAGAAA AGAGGAAGAA 
GAAAAACAAA AGCGACTCCA GGAAGAAAAA ACACAAGAAA AAGTTCAAGA AGAGGAACGG 
AAGGCTGAGG AGAAACCATG TAAGGGTGAG CCAGCTACTG CTATGGTGAA TTATAGAGCA 
TTGTACCGCT TTGAAGCCAG GAGCCATGAT GAGATGAGTT TTAATTCCGG GGATATAATA 
CAGGTTGATG AAAAAACCGT AGGAGAACCT GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT 
TTTGGCTGGT TTCCTTGCAA CTATGTAGAA AAAATGCCAT CAGGTGAAAA ATCTGTGTCT 
CCTAAGAAGG CCTTACTTCC CCCTACAGTA TCAGTATCTG CTACCTCAAC TTCTTCTGAA 
TCACTTTCTT CAAATCAACC AGCATCAGTG ACTGATTATC AAAATGTATC TTTTACAAAC 
CTAACTGTTA ATACATCATG GCAGAAAAAA TCAGCTTTTA CTCGTACTGT TTCCCCTGGA 
TCTGTATCAC CGATTCATGG CCAGGGACAG GTTGTAGAAA ACCTAAAGGC GCAGGCCCTG 
TGTTCTTGGA CTGCAAAGAA GGATAACCAC TTGAACTTTT CAAAACATGA TATTATCACT 
GTGTTGGAAC AGCAAGAAAA TTGGTGGTTT GGAGAGGTGC ATGGAGGAAG AGGGTGGTTT 
CCAAAATCTT ATGTCAAAAT ACTCCCTGGG AGTGAAGTCA AGCGGGAAGA ACCAGAAGCT 
TTGTATGCAG CTGTAAACAA GAAACCTACC TCTGCAGCCT ACACAGGTGG AGAGGAATAT 
ATTGCACTTT ATCCATATTC AAGTGTAGAA CCTGGAGATT TGACTTTCAC TGAAGGTGAA 
GAAATATTGG TGACCCAGAA AGATGGAGAA TGGTGGACAG GAAGTATTGG AGATAGAACT 
GGAATTTTTC CATCAAATTA TGTCAAACCA AAAGATCAAG AGAGTTTTGG GAGTGCTAGC 
AAATCTGGAA CATCAAACAA GAAACCTGAG ATTGCTCAAG TAACTTCAGC ATATGTGGCT 
TCTGGTTCTG AACAACTTAG CCTTGCACCC GGGCAGTTAA TACTAATCCT AAAGAAAAAT 
TCAAGTGGGT GGTGGCAAGG GGAATTACAG GCCAGAGGAA AAAAGCGACA GAAAGGATGG 
TTTCCTGCCA GCCATGTTAA ACTTTTGGGT CCAAGTAGTG AAAGAACTAC ACCTGCCTTT 
CATCCTGTAT GTCAGGTGAT TGCTATGTAT GACTATGTAG CAAATAACGA GGATGAGCTG 
AATTTCTCCA AGGGACAACT TATCAATGTT TTGAACAAAG ATGATCCTGA CTGGTGGCAA 
GGAGAAATCA ACGGGGTGAC TGGTCTCTTT CCTTCAAACT ATGTTAAGAT GACAACAGAC 
TCCGATCCAA GTCAACAGTG GTGTGCTGAT CTCCAAAGCC TGGACACCAT GCAGCCAGTT 
GAGAGGAAGA GGCAGGGCTA TATTCACGAG CTGATTCAGA CCGAAGAGCG GTACGTGGAC 
GACCTTCAGC TCGTCGTGGA GGTTTTTCAG AAACGGATGG TGGAGTCGGG CTTTCTCAGT 
GAAGGAGAAA TGGCCTTGAT TTTTGTAAAC TGGAAAGAAC TCATCATGTC TAACACAAAG 
CTACTCAAGG CCTTGCGGGT ACGGAAGAAG ACCGGGGGAG AGAAGATGCC CGTGCAGATG 
ATTGGGGACA TCCTGGCAGC CGAGCTGTCC CACATGCAGG CTTATATCCG GTTCTGTAGC 
TGTCAGCTTA ATGGAGCAGC TCTGTTACAG CAGAAGACCG ATGAAGACAC GGATTTCAAG 
GAATTTTTAA AGAAGCTGGC ATCTGACCCT CGATGTAAAG GAATGCCCCT TTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG AATCACCCGC TACCCTCTGC TCATCAGAAG TATTCTGGAG 
AACACCCTGG AGAGTCATGT GGACCATTCC TCCCTAAAGC TGGCTCTTGA GCGGGCAGAG 
GAGCTTTGTT CCCAAGTTAA TGAAGGAGTT CGGGAAAAAG AAAATTCAGA CCGACTCGAG 
TGGATCCAGG CACACGTTCA GTGTGAAGGT CTTGCAGAGC AACTTATTTT CAACTCCCTC 
ACCAACTGCC TGGGGCCCCG GAAGCTTCTG CACAGTGGGA AACTATACAA GACCAAGAGC 
AACAAGGAAC TACACGGATT CCTCTTCAAT GACTTTCTGC TTCTTACCTA CATGGTCAAG 
CAGTTTGCTG TTTCCTCAGG TTCTGAGAAG CTTTTCAGCT CTAAGTCCAA TGCCCAATTC 
AAAATGTATA AAACGCCCAT TTTCCTGAAT GAAGTCCTGG TGAAACTGCC CACAGACCCT 
TCCAGCGATG AGCCTGTCTT CCACATTTCT CACATTGACC GGGTCTACAC CCTCCGAACA 
GACAACATTA ACGAGAGGAC TGCTTGGGTC CAGAAGATCA AGGCGGCATC TGAGCAGTAC 
ATTGACACGG AAAAGAAGAA ACGGGAGAAG GCTTATCAAG CTCGCTCTCA AAAGACTTCA 
GGCATTGGGC GTCTGATGGT GCACGTCATC GAAGCTACAG AATTAAAAGC CTGTAAACCG 
AATGGAAAGA GCAATCCATA CTGTGAAATC AGCATGGGCT CCCAGAGCTA TACCACCAGA 
ACCCTCCAGG ACACACTCAA TCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAC 
CTTTACCAAG ATGTGCTGTG TCTCACCATG TTTGACAGAG ACCAGTTTTC ACCAGATGAT 
TTCCTGGGTC GCACTGAAGT TCCAGTGGCA AAAATTCGCA CAGAACAGGA AAGCAAAGGC 
CCCACAACCC GCCGACTCCT GCTGCACGAG GTCCCCACCG GGGAGGTCTG GGTCCGCTTT 
GACCTGCAGC TTTTTGAGCA AAAAACTCTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827837.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683396.2 Felis catus intersectin 2 (ITSN2), transcript variant X14, mRNA.

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CloneID	OFb73984
Clone ID Related Accession (Same CDS sequence)	XM_019827837.2
Accession Version	XM_019827837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019827837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA ACTTTATAAG 
ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAGAAG 
AAACTAGAAG ATGAGGCTGT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGAGAA 
AACCTTAGAA AAGAGGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AACACAAGAA 
AAAGTTCAAG AAGAGGAACG GAAGGCTGAG GAGAAACCAT GTAAGGGTGA GCCAGCTACT 
GCTATGGTGA ATTATAGAGC ATTGTACCGC TTTGAAGCCA GGAGCCATGA TGAGATGAGT 
TTTAATTCCG GGGATATAAT ACAGGTTGAT GAAAAAACCG TAGGAGAACC TGGTTGGCTT 
TATGGTAGTT TTCAAGGAAA TTTTGGCTGG TTTCCTTGCA ACTATGTAGA AAAAATGCCA 
TCAGGTGAAA AATCTGTGTC TCCTAAGAAG GCCTTACTTC CCCCTACAGT ATCAGTATCT 
GCTACCTCAA CTTCTTCTGA ATCACTTTCT TCAAATCAAC CAGCATCAGT GACTGATTAT 
CAAAATGTAT CTTTTACAAA CCTAACTGTT AATACATCAT GGCAGAAAAA ATCAGCTTTT 
ACTCGTACTG TTTCCCCTGG ATCTGTATCA CCGATTCATG GCCAGGGACA GGTTGTAGAA 
AACCTAAAGG CGCAGGCCCT GTGTTCTTGG ACTGCAAAGA AGGATAACCA CTTGAACTTT 
TCAAAACATG ATATTATCAC TGTGTTGGAA CAGCAAGAAA ATTGGTGGTT TGGAGAGGTG 
CATGGAGGAA GAGGGTGGTT TCCAAAATCT TATGTCAAAA TACTCCCTGG GAGTGAAGTC 
AAGCGGGAAG AACCAGAAGC TTTGTATGCA GCTGTAAACA AGAAACCTAC CTCTGCAGCC 
TACACAGGTG GAGAGGAATA TATTGCACTT TATCCATATT CAAGTGTAGA ACCTGGAGAT 
TTGACTTTCA CTGAAGGTGA AGAAATATTG GTGACCCAGA AAGATGGAGA ATGGTGGACA 
GGAAGTATTG GAGATAGAAC TGGAATTTTT CCATCAAATT ATGTCAAACC AAAAGATCAA 
GAGAGTTTTG GGAGTGCTAG CAAATCTGGA ACATCAAACA AGAAACCTGA GATTGCTCAA 
GTAACTTCAG CATATGTGGC TTCTGGTTCT GAACAACTTA GCCTTGCACC CGGGCAGTTA 
ATACTAATCC TAAAGAAAAA TTCAAGTGGG TGGTGGCAAG GGGAATTACA GGCCAGAGGA 
AAAAAGCGAC AGAAAGGATG GTTTCCTGCC AGCCATGTTA AACTTTTGGG TCCAAGTAGT 
GAAAGAACTA CACCTGCCTT TCATCCTGTA TGTCAGGTGA TTGCTATGTA TGACTATGTA 
GCAAATAACG AGGATGAGCT GAATTTCTCC AAGGGACAAC TTATCAATGT TTTGAACAAA 
GATGATCCTG ACTGGTGGCA AGGAGAAATC AACGGGGTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCCGATCCA AGTCAACAGT GGTGTGCTGA TCTCCAAAGC 
CTGGACACCA TGCAGCCAGT TGAGAGGAAG AGGCAGGGCT ATATTCACGA GCTGATTCAG 
ACCGAAGAGC GGTACGTGGA CGACCTTCAG CTCGTCGTGG AGGTTTTTCA GAAACGGATG 
GTGGAGTCGG GCTTTCTCAG TGAAGGAGAA ATGGCCTTGA TTTTTGTAAA CTGGAAAGAA 
CTCATCATGT CTAACACAAA GCTACTCAAG GCCTTGCGGG TACGGAAGAA GACCGGGGGA 
GAGAAGATGC CCGTGCAGAT GATTGGGGAC ATCCTGGCAG CCGAGCTGTC CCACATGCAG 
GCTTATATCC GGTTCTGTAG CTGTCAGCTT AATGGAGCAG CTCTGTTACA GCAGAAGACC 
GATGAAGACA CGGATTTCAA GGAATTTTTA AAGAAGCTGG CATCTGACCC TCGATGTAAA 
GGAATGCCCC TTTCCAGCTT CCTGCTGAAG CCCATGCAGA GAATCACCCG CTACCCTCTG 
CTCATCAGAA GTATTCTGGA GAACACCCTG GAGAGTCATG TGGACCATTC CTCCCTAAAG 
CTGGCTCTTG AGCGGGCAGA GGAGCTTTGT TCCCAAGTTA ATGAAGGAGT TCGGGAAAAA 
GAAAATTCAG ACCGACTCGA GTGGATCCAG GCACACGTTC AGTGTGAAGG TCTTGCAGAG 
CAACTTATTT TCAACTCCCT CACCAACTGC CTGGGGCCCC GGAAGCTTCT GCACAGTGGG 
AAACTATACA AGACCAAGAG CAACAAGGAA CTACACGGAT TCCTCTTCAA TGACTTTCTG 
CTTCTTACCT ACATGGTCAA GCAGTTTGCT GTTTCCTCAG GTTCTGAGAA GCTTTTCAGC 
TCTAAGTCCA ATGCCCAATT CAAAATGTAT AAAACGCCCA TTTTCCTGAA TGAAGTCCTG 
GTGAAACTGC CCACAGACCC TTCCAGCGAT GAGCCTGTCT TCCACATTTC TCACATTGAC 
CGGGTCTACA CCCTCCGAAC AGACAACATT AACGAGAGGA CTGCTTGGGT CCAGAAGATC 
AAGGCGGCAT CTGAGCAGTA CATTGACACG GAAAAGAAGA AACGGGAGAA GGCTTATCAA 
GCTCGCTCTC AAAAGACTTC AGGCATTGGG CGTCTGATGG TGCACGTCAT CGAAGCTACA 
GAATTAAAAG CCTGTAAACC GAATGGAAAG AGCAATCCAT ACTGTGAAAT CAGCATGGGC 
TCCCAGAGCT ATACCACCAG AACCCTCCAG GACACACTCA ATCCCAAGTG GAACTTTAAC 
TGCCAGTTCT TTATTAAGGA CCTTTACCAA GATGTGCTGT GTCTCACCAT GTTTGACAGA 
GACCAGTTTT CACCAGATGA TTTCCTGGGT CGCACTGAAG TTCCAGTGGC AAAAATTCGC 
ACAGAACAGG AAAGCAAAGG CCCCACAACC CGCCGACTCC TGCTGCACGA GGTCCCCACC 
GGGGAGGTCT GGGTCCGCTT TGACCTGCAG CTTTTTGAGC AAAAAACTCT CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683396.2
CDS	34..5010
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827837.2
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827837.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA ACTTTATAAG 
ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAGAAG 
AAACTAGAAG ATGAGGCTGT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGAGAA 
AACCTTAGAA AAGAGGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AACACAAGAA 
AAAGTTCAAG AAGAGGAACG GAAGGCTGAG GAGAAACCAT GTAAGGGTGA GCCAGCTACT 
GCTATGGTGA ATTATAGAGC ATTGTACCGC TTTGAAGCCA GGAGCCATGA TGAGATGAGT 
TTTAATTCCG GGGATATAAT ACAGGTTGAT GAAAAAACCG TAGGAGAACC TGGTTGGCTT 
TATGGTAGTT TTCAAGGAAA TTTTGGCTGG TTTCCTTGCA ACTATGTAGA AAAAATGCCA 
TCAGGTGAAA AATCTGTGTC TCCTAAGAAG GCCTTACTTC CCCCTACAGT ATCAGTATCT 
GCTACCTCAA CTTCTTCTGA ATCACTTTCT TCAAATCAAC CAGCATCAGT GACTGATTAT 
CAAAATGTAT CTTTTACAAA CCTAACTGTT AATACATCAT GGCAGAAAAA ATCAGCTTTT 
ACTCGTACTG TTTCCCCTGG ATCTGTATCA CCGATTCATG GCCAGGGACA GGTTGTAGAA 
AACCTAAAGG CGCAGGCCCT GTGTTCTTGG ACTGCAAAGA AGGATAACCA CTTGAACTTT 
TCAAAACATG ATATTATCAC TGTGTTGGAA CAGCAAGAAA ATTGGTGGTT TGGAGAGGTG 
CATGGAGGAA GAGGGTGGTT TCCAAAATCT TATGTCAAAA TACTCCCTGG GAGTGAAGTC 
AAGCGGGAAG AACCAGAAGC TTTGTATGCA GCTGTAAACA AGAAACCTAC CTCTGCAGCC 
TACACAGGTG GAGAGGAATA TATTGCACTT TATCCATATT CAAGTGTAGA ACCTGGAGAT 
TTGACTTTCA CTGAAGGTGA AGAAATATTG GTGACCCAGA AAGATGGAGA ATGGTGGACA 
GGAAGTATTG GAGATAGAAC TGGAATTTTT CCATCAAATT ATGTCAAACC AAAAGATCAA 
GAGAGTTTTG GGAGTGCTAG CAAATCTGGA ACATCAAACA AGAAACCTGA GATTGCTCAA 
GTAACTTCAG CATATGTGGC TTCTGGTTCT GAACAACTTA GCCTTGCACC CGGGCAGTTA 
ATACTAATCC TAAAGAAAAA TTCAAGTGGG TGGTGGCAAG GGGAATTACA GGCCAGAGGA 
AAAAAGCGAC AGAAAGGATG GTTTCCTGCC AGCCATGTTA AACTTTTGGG TCCAAGTAGT 
GAAAGAACTA CACCTGCCTT TCATCCTGTA TGTCAGGTGA TTGCTATGTA TGACTATGTA 
GCAAATAACG AGGATGAGCT GAATTTCTCC AAGGGACAAC TTATCAATGT TTTGAACAAA 
GATGATCCTG ACTGGTGGCA AGGAGAAATC AACGGGGTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCCGATCCA AGTCAACAGT GGTGTGCTGA TCTCCAAAGC 
CTGGACACCA TGCAGCCAGT TGAGAGGAAG AGGCAGGGCT ATATTCACGA GCTGATTCAG 
ACCGAAGAGC GGTACGTGGA CGACCTTCAG CTCGTCGTGG AGGTTTTTCA GAAACGGATG 
GTGGAGTCGG GCTTTCTCAG TGAAGGAGAA ATGGCCTTGA TTTTTGTAAA CTGGAAAGAA 
CTCATCATGT CTAACACAAA GCTACTCAAG GCCTTGCGGG TACGGAAGAA GACCGGGGGA 
GAGAAGATGC CCGTGCAGAT GATTGGGGAC ATCCTGGCAG CCGAGCTGTC CCACATGCAG 
GCTTATATCC GGTTCTGTAG CTGTCAGCTT AATGGAGCAG CTCTGTTACA GCAGAAGACC 
GATGAAGACA CGGATTTCAA GGAATTTTTA AAGAAGCTGG CATCTGACCC TCGATGTAAA 
GGAATGCCCC TTTCCAGCTT CCTGCTGAAG CCCATGCAGA GAATCACCCG CTACCCTCTG 
CTCATCAGAA GTATTCTGGA GAACACCCTG GAGAGTCATG TGGACCATTC CTCCCTAAAG 
CTGGCTCTTG AGCGGGCAGA GGAGCTTTGT TCCCAAGTTA ATGAAGGAGT TCGGGAAAAA 
GAAAATTCAG ACCGACTCGA GTGGATCCAG GCACACGTTC AGTGTGAAGG TCTTGCAGAG 
CAACTTATTT TCAACTCCCT CACCAACTGC CTGGGGCCCC GGAAGCTTCT GCACAGTGGG 
AAACTATACA AGACCAAGAG CAACAAGGAA CTACACGGAT TCCTCTTCAA TGACTTTCTG 
CTTCTTACCT ACATGGTCAA GCAGTTTGCT GTTTCCTCAG GTTCTGAGAA GCTTTTCAGC 
TCTAAGTCCA ATGCCCAATT CAAAATGTAT AAAACGCCCA TTTTCCTGAA TGAAGTCCTG 
GTGAAACTGC CCACAGACCC TTCCAGCGAT GAGCCTGTCT TCCACATTTC TCACATTGAC 
CGGGTCTACA CCCTCCGAAC AGACAACATT AACGAGAGGA CTGCTTGGGT CCAGAAGATC 
AAGGCGGCAT CTGAGCAGTA CATTGACACG GAAAAGAAGA AACGGGAGAA GGCTTATCAA 
GCTCGCTCTC AAAAGACTTC AGGCATTGGG CGTCTGATGG TGCACGTCAT CGAAGCTACA 
GAATTAAAAG CCTGTAAACC GAATGGAAAG AGCAATCCAT ACTGTGAAAT CAGCATGGGC 
TCCCAGAGCT ATACCACCAG AACCCTCCAG GACACACTCA ATCCCAAGTG GAACTTTAAC 
TGCCAGTTCT TTATTAAGGA CCTTTACCAA GATGTGCTGT GTCTCACCAT GTTTGACAGA 
GACCAGTTTT CACCAGATGA TTTCCTGGGT CGCACTGAAG TTCCAGTGGC AAAAATTCGC 
ACAGAACAGG AAAGCAAAGG CCCCACAACC CGCCGACTCC TGCTGCACGA GGTCCCCACC 
GGGGAGGTCT GGGTCCGCTT TGACCTGCAG CTTTTTGAGC AAAAAACTCT CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_023252059.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107827.1 Felis catus intersectin 2 (ITSN2), transcript variant X15, mRNA.

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CloneID	OFb73985
Clone ID Related Accession (Same CDS sequence)	XM_023252059.1
Accession Version	XM_023252059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	intersectin-2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA ACTTTATAAG 
ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAGAAG 
AAACTAGAAG ATGAGGCTGT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGAGAA 
AACCTTAGAA AAGAGGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AACACAAGAA 
AAAGTTCAAG AAGAGGAACG GAAGGCTGAG GAGAAACCAT GTGAGCCAGC TACTGCTATG 
GTGAATTATA GAGCATTGTA CCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT 
TCCGGGGATA TAATACAGGT TGATGAAAAA ACCGTAGGAG AACCTGGTTG GCTTTATGGT 
AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCCATCAGGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCAGT ATCTGCTACC 
TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT 
ACTGTTTCCC CTGGATCTGT ATCACCGATT CATGGCCAGG GACAGGTTGT AGAAAACCTA 
AAGGCGCAGG CCCTGTGTTC TTGGACTGCA AAGAAGGATA ACCACTTGAA CTTTTCAAAA 
CATGATATTA TCACTGTGTT GGAACAGCAA GAAAATTGGT GGTTTGGAGA GGTGCATGGA 
GGAAGAGGGT GGTTTCCAAA ATCTTATGTC AAAATACTCC CTGGGAGTGA AGTCAAGCGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTACACA 
GGTGGAGAGG AATATATTGC ACTTTATCCA TATTCAAGTG TAGAACCTGG AGATTTGACT 
TTCACTGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAATGGTG GACAGGAAGT 
ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAATC TGGAACATCA AACAAGAAAC CTGAGATTGC TCAAGTAACT 
TCAGCATATG TGGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCCGGGCA GTTAATACTA 
ATCCTAAAGA AAAATTCAAG TGGGTGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
ACTACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGTAGCAAAT 
AACGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT 
CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT 
AAGATGACAA CAGACTCCGA TCCAAGTCAA CAGTGGTGTG CTGATCTCCA AAGCCTGGAC 
ACCATGCAGC CAGTTGAGAG GAAGAGGCAG GGCTATATTC ACGAGCTGAT TCAGACCGAA 
GAGCGGTACG TGGACGACCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGTGGAG 
TCGGGCTTTC TCAGTGAAGG AGAAATGGCC TTGATTTTTG TAAACTGGAA AGAACTCATC 
ATGTCTAACA CAAAGCTACT CAAGGCCTTG CGGGTACGGA AGAAGACCGG GGGAGAGAAG 
ATGCCCGTGC AGATGATTGG GGACATCCTG GCAGCCGAGC TGTCCCACAT GCAGGCTTAT 
ATCCGGTTCT GTAGCTGTCA GCTTAATGGA GCAGCTCTGT TACAGCAGAA GACCGATGAA 
GACACGGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGATG TAAAGGAATG 
CCCCTTTCCA GCTTCCTGCT GAAGCCCATG CAGAGAATCA CCCGCTACCC TCTGCTCATC 
AGAAGTATTC TGGAGAACAC CCTGGAGAGT CATGTGGACC ATTCCTCCCT AAAGCTGGCT 
CTTGAGCGGG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT 
TCAGACCGAC TCGAGTGGAT CCAGGCACAC GTTCAGTGTG AAGGTCTTGC AGAGCAACTT 
ATTTTCAACT CCCTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAACTA 
TACAAGACCA AGAGCAACAA GGAACTACAC GGATTCCTCT TCAATGACTT TCTGCTTCTT 
ACCTACATGG TCAAGCAGTT TGCTGTTTCC TCAGGTTCTG AGAAGCTTTT CAGCTCTAAG 
TCCAATGCCC AATTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CCTGGTGAAA 
CTGCCCACAG ACCCTTCCAG CGATGAGCCT GTCTTCCACA TTTCTCACAT TGACCGGGTC 
TACACCCTCC GAACAGACAA CATTAACGAG AGGACTGCTT GGGTCCAGAA GATCAAGGCG 
GCATCTGAGC AGTACATTGA CACGGAAAAG AAGAAACGGG AGAAGGCTTA TCAAGCTCGC 
TCTCAAAAGA CTTCAGGCAT TGGGCGTCTG ATGGTGCACG TCATCGAAGC TACAGAATTA 
AAAGCCTGTA AACCGAATGG AAAGAGCAAT CCATACTGTG AAATCAGCAT GGGCTCCCAG 
AGCTATACCA CCAGAACCCT CCAGGACACA CTCAATCCCA AGTGGAACTT TAACTGCCAG 
TTCTTTATTA AGGACCTTTA CCAAGATGTG CTGTGTCTCA CCATGTTTGA CAGAGACCAG 
TTTTCACCAG ATGATTTCCT GGGTCGCACT GAAGTTCCAG TGGCAAAAAT TCGCACAGAA 
CAGGAAAGCA AAGGCCCCAC AACCCGCCGA CTCCTGCTGC ACGAGGTCCC CACCGGGGAG 
GTCTGGGTCC GCTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107827.1
CDS	136..5106
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCEPATAMVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_023252059.1
Organism	Felis catus(domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252059.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA ACTTTATAAG 
ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAGAAG 
AAACTAGAAG ATGAGGCTGT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGAGAA 
AACCTTAGAA AAGAGGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AACACAAGAA 
AAAGTTCAAG AAGAGGAACG GAAGGCTGAG GAGAAACCAT GTGAGCCAGC TACTGCTATG 
GTGAATTATA GAGCATTGTA CCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT 
TCCGGGGATA TAATACAGGT TGATGAAAAA ACCGTAGGAG AACCTGGTTG GCTTTATGGT 
AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCCATCAGGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCAGT ATCTGCTACC 
TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT 
ACTGTTTCCC CTGGATCTGT ATCACCGATT CATGGCCAGG GACAGGTTGT AGAAAACCTA 
AAGGCGCAGG CCCTGTGTTC TTGGACTGCA AAGAAGGATA ACCACTTGAA CTTTTCAAAA 
CATGATATTA TCACTGTGTT GGAACAGCAA GAAAATTGGT GGTTTGGAGA GGTGCATGGA 
GGAAGAGGGT GGTTTCCAAA ATCTTATGTC AAAATACTCC CTGGGAGTGA AGTCAAGCGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTACACA 
GGTGGAGAGG AATATATTGC ACTTTATCCA TATTCAAGTG TAGAACCTGG AGATTTGACT 
TTCACTGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAATGGTG GACAGGAAGT 
ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAATC TGGAACATCA AACAAGAAAC CTGAGATTGC TCAAGTAACT 
TCAGCATATG TGGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCCGGGCA GTTAATACTA 
ATCCTAAAGA AAAATTCAAG TGGGTGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
ACTACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGTAGCAAAT 
AACGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT 
CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT 
AAGATGACAA CAGACTCCGA TCCAAGTCAA CAGTGGTGTG CTGATCTCCA AAGCCTGGAC 
ACCATGCAGC CAGTTGAGAG GAAGAGGCAG GGCTATATTC ACGAGCTGAT TCAGACCGAA 
GAGCGGTACG TGGACGACCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGTGGAG 
TCGGGCTTTC TCAGTGAAGG AGAAATGGCC TTGATTTTTG TAAACTGGAA AGAACTCATC 
ATGTCTAACA CAAAGCTACT CAAGGCCTTG CGGGTACGGA AGAAGACCGG GGGAGAGAAG 
ATGCCCGTGC AGATGATTGG GGACATCCTG GCAGCCGAGC TGTCCCACAT GCAGGCTTAT 
ATCCGGTTCT GTAGCTGTCA GCTTAATGGA GCAGCTCTGT TACAGCAGAA GACCGATGAA 
GACACGGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGATG TAAAGGAATG 
CCCCTTTCCA GCTTCCTGCT GAAGCCCATG CAGAGAATCA CCCGCTACCC TCTGCTCATC 
AGAAGTATTC TGGAGAACAC CCTGGAGAGT CATGTGGACC ATTCCTCCCT AAAGCTGGCT 
CTTGAGCGGG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT 
TCAGACCGAC TCGAGTGGAT CCAGGCACAC GTTCAGTGTG AAGGTCTTGC AGAGCAACTT 
ATTTTCAACT CCCTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAACTA 
TACAAGACCA AGAGCAACAA GGAACTACAC GGATTCCTCT TCAATGACTT TCTGCTTCTT 
ACCTACATGG TCAAGCAGTT TGCTGTTTCC TCAGGTTCTG AGAAGCTTTT CAGCTCTAAG 
TCCAATGCCC AATTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CCTGGTGAAA 
CTGCCCACAG ACCCTTCCAG CGATGAGCCT GTCTTCCACA TTTCTCACAT TGACCGGGTC 
TACACCCTCC GAACAGACAA CATTAACGAG AGGACTGCTT GGGTCCAGAA GATCAAGGCG 
GCATCTGAGC AGTACATTGA CACGGAAAAG AAGAAACGGG AGAAGGCTTA TCAAGCTCGC 
TCTCAAAAGA CTTCAGGCAT TGGGCGTCTG ATGGTGCACG TCATCGAAGC TACAGAATTA 
AAAGCCTGTA AACCGAATGG AAAGAGCAAT CCATACTGTG AAATCAGCAT GGGCTCCCAG 
AGCTATACCA CCAGAACCCT CCAGGACACA CTCAATCCCA AGTGGAACTT TAACTGCCAG 
TTCTTTATTA AGGACCTTTA CCAAGATGTG CTGTGTCTCA CCATGTTTGA CAGAGACCAG 
TTTTCACCAG ATGATTTCCT GGGTCGCACT GAAGTTCCAG TGGCAAAAAT TCGCACAGAA 
CAGGAAAGCA AAGGCCCCAC AACCCGCCGA CTCCTGCTGC ACGAGGTCCC CACCGGGGAG 
GTCTGGGTCC GCTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827837.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683396.1 Felis catus intersectin 2 (ITSN2), transcript variant X7, mRNA.

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CloneID	OFb32214
Clone ID Related Accession (Same CDS sequence)	XM_019827837.1
Accession Version	XM_019827837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA ACTTTATAAG 
ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAGAAG 
AAACTAGAAG ATGAGGCTGT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGAGAA 
AACCTTAGAA AAGAGGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AACACAAGAA 
AAAGTTCAAG AAGAGGAACG GAAGGCTGAG GAGAAACCAT GTAAGGGTGA GCCAGCTACT 
GCTTTGGTGA ATTATAGAGC ATTGTACCGC TTTGAAGCCA GGAGCCATGA TGAGATGAGT 
TTTAATTCCG GGGATATAAT ACAGGTTGAT GAAAAAACCG TAGGAGAACC TGGTTGGCTT 
TATGGTAGTT TTCAAGGAAA TTTTGGCTGG TTTCCTTGCA ACTATGTAGA AAAAATGCCA 
TCAGGTGAAA AATCTGTGTC TCCTAAGAAG GCCTTACTTC CCCCTACAGT ATCAGTATCT 
GCTACCTCAA CTTCTTCTGA ATCACTTTCT TCAAATCAAC CAGCATCAGT GACTGATTAT 
CAAAATGTAT CTTTTACAAA CCTAACTGTT AATACATCAT GGCAGAAAAA ATCAGCTTTT 
ACTCGTACTG TTTCCCCTGG ATCTGTATCA CCGATTCATG GCCAGGGACA GGTTGTAGAA 
AACCTAAAGG CGCAGGCCCT GTGTTCTTGG ACTGCAAAGA AGGATAACCA CTTGAACTTT 
TCAAAACATG ATATTATCAC TGTGTTGGAA CAGCAAGAAA ATTGGTGGTT TGGAGAGGTG 
CATGGAGGAA GAGGGTGGTT TCCAAAATCT TATGTCAAAA TACTCCCTGG GAGTGAAGTC 
AAGCGGGAAG AACCAGAAGC TTTGTATGCA GCTGTAAACA AGAAACCTAC CTCTGCAGCC 
TACACAGGTG GAGAGGAATA TATTGCACTT TATCCATATT CAAGTGTAGA ACCTGGAGAT 
TTGACTTTCA CTGAAGGTGA AGAAATATTG GTGACCCAGA AAGATGGAGA ATGGTGGACA 
GGAAGTATTG GAGATAGAAC TGGAATTTTT CCATCAAATT ATGTCAAACC AAAAGATCAA 
GAGAGTTTTG GGAGTGCTAG CAAATCTGGA ACATCAAACA AGAAACCTGA GATTGCTCAA 
GTAACTTCAG CATATGTGGC TTCTGGTTCT GAACAACTTA GCCTTGCACC CGGGCAGTTA 
ATACTAATCC TAAAGAAAAA TTCAAGTGGG TGGTGGCAAG GGGAATTACA GGCCAGAGGA 
AAAAAGCGAC AGAAAGGATG GTTTCCTGCC AGCCATGTTA AACTTTTGGG TCCAAGTAGT 
GAAAGAACTA CACCTGCCTT TCATCCTGTA TGTCAGGTGA TTGCTATGTA TGACTATGTA 
GCAAATAACG AGGATGAGCT GAATTTCTCC AAGGGACAAC TTATCAATGT TTTGAACAAA 
GATGATCCTG ACTGGTGGCA AGGAGAAATC AACGGGGTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCCGATCCA AGTCAACAGT GGTGTGCTGA TCTCCAAAGC 
CTGGACACCA TGCAGCCAGT TGAGAGGAAG AGGCAGGGCT ATATTCACGA GCTGATTCAG 
ACCGAAGAGC GGTACGTGGA CGACCTTCAG CTCGTCGTGG AGGTTTTTCA GAAACGGATG 
GTGGAGTCGG GCTTTCTCAG TGAAGGAGAA ATGGCCTTGA TTTTTGTAAA CTGGAAAGAA 
CTCATCATGT CTAACACAAA GCTACTCAAG GCCTTGCGGG TACGGAAGAA GACCGGGGGA 
GAGAAGATGC CCGTGCAGAT GATTGGGGAC ATCCTGGCAG CCGAGCTGTC CCACATGCAG 
GCTTATATCC GGTTCTGTAG CTGTCAGCTT AATGGAGCAG CTCTGTTACA GCAGAAGACC 
GATGAAGACA CGGATTTCAA GGAATTTTTA AAGAAGCTGG CATCTGACCC TCGATGTAAA 
GGAATGCCCC TTTCCAGCTT CCTGCTGAAG CCCATGCAGA GAATCACCCG CTACCCTCTG 
CTCATCAGAA GTATTCTGGA GAACACCCTG GAGAGTCATG TGGACCATTC CTCCCTAAAG 
CTGGCTCTTG AGCGGGCAGA GGAGCTTTGT TCCCAAGTTA ATGAAGGAGT TCGGGAAAAA 
GAAAATTCAG ACCGACTCGA GTGGATCCAG GCACACGTTC AGTGTGAAGG TCTTGCAGAG 
CAACTTATTT TCAACTCCCT CACCAACTGC CTGGGGCCCC GGAAGCTTCT GCACAGTGGG 
AAACTATACA AGACCAAGAG CAACAAGGAA CTACACGGAT TCCTCTTCAA TGACTTTCTG 
CTTCTTACCT ACATGGTCAA GCAGTTTGCT GTTTCCTCAG GTTCTGAGAA GCTTTTCAGC 
TCTAAGTCCA ATGCCCAATT CAAAATGTAT AAAACGCCCA TTTTCCTGAA TGAAGTCCTG 
GTGAAACTGC CCACAGACCC TTCCAGCGAT GAGCCTGTCT TCCACATTTC TCACATTGAC 
CGGGTCTACA CCCTCCGAAC AGACAACATT AACGAGAGGA CTGCTTGGGT CCAGAAGATC 
AAGGCGGCAT CTGAGCAGTA CATTGACACG GAAAAGAAGA AACGGGAGAA GGCTTATCAA 
GCTCGCTCTC AAAAGACTTC AGGCATTGGG CGTCTGATGG TGCACGTCAT CGAAGCTACA 
GAATTAAAAG CCTGTAAACC GAATGGAAAG AGCAATCCAT ACTGTGAAAT CAGCATGGGC 
TCCCAGAGCT ATACCACCAG AACCCTCCAG GACACACTCA ATCCCAAGTG GAACTTTAAC 
TGCCAGTTCT TTATTAAGGA CCTTTACCAA GATGTGCTGT GTCTCACCAT GTTTGACAGA 
GACCAGTTTT CACCAGATGA TTTCCTGGGT CGCACTGAAG TTCCAGTGGC AAAAATTCGC 
ACAGAACAGG AAAGCAAAGG CCCCACAACC CGCCGACTCC TGCTGCACGA GGTCCCCACC 
GGGGAGGTCT GGGTCCGCTT TGACCTGCAG CTTTTTGAGC AAAAAACTCT CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683396.1
CDS	16..4992
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827837.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827837.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA ACTTTATAAG 
ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAGAAG 
AAACTAGAAG ATGAGGCTGT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGAGAA 
AACCTTAGAA AAGAGGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AACACAAGAA 
AAAGTTCAAG AAGAGGAACG GAAGGCTGAG GAGAAACCAT GTAAGGGTGA GCCAGCTACT 
GCTTTGGTGA ATTATAGAGC ATTGTACCGC TTTGAAGCCA GGAGCCATGA TGAGATGAGT 
TTTAATTCCG GGGATATAAT ACAGGTTGAT GAAAAAACCG TAGGAGAACC TGGTTGGCTT 
TATGGTAGTT TTCAAGGAAA TTTTGGCTGG TTTCCTTGCA ACTATGTAGA AAAAATGCCA 
TCAGGTGAAA AATCTGTGTC TCCTAAGAAG GCCTTACTTC CCCCTACAGT ATCAGTATCT 
GCTACCTCAA CTTCTTCTGA ATCACTTTCT TCAAATCAAC CAGCATCAGT GACTGATTAT 
CAAAATGTAT CTTTTACAAA CCTAACTGTT AATACATCAT GGCAGAAAAA ATCAGCTTTT 
ACTCGTACTG TTTCCCCTGG ATCTGTATCA CCGATTCATG GCCAGGGACA GGTTGTAGAA 
AACCTAAAGG CGCAGGCCCT GTGTTCTTGG ACTGCAAAGA AGGATAACCA CTTGAACTTT 
TCAAAACATG ATATTATCAC TGTGTTGGAA CAGCAAGAAA ATTGGTGGTT TGGAGAGGTG 
CATGGAGGAA GAGGGTGGTT TCCAAAATCT TATGTCAAAA TACTCCCTGG GAGTGAAGTC 
AAGCGGGAAG AACCAGAAGC TTTGTATGCA GCTGTAAACA AGAAACCTAC CTCTGCAGCC 
TACACAGGTG GAGAGGAATA TATTGCACTT TATCCATATT CAAGTGTAGA ACCTGGAGAT 
TTGACTTTCA CTGAAGGTGA AGAAATATTG GTGACCCAGA AAGATGGAGA ATGGTGGACA 
GGAAGTATTG GAGATAGAAC TGGAATTTTT CCATCAAATT ATGTCAAACC AAAAGATCAA 
GAGAGTTTTG GGAGTGCTAG CAAATCTGGA ACATCAAACA AGAAACCTGA GATTGCTCAA 
GTAACTTCAG CATATGTGGC TTCTGGTTCT GAACAACTTA GCCTTGCACC CGGGCAGTTA 
ATACTAATCC TAAAGAAAAA TTCAAGTGGG TGGTGGCAAG GGGAATTACA GGCCAGAGGA 
AAAAAGCGAC AGAAAGGATG GTTTCCTGCC AGCCATGTTA AACTTTTGGG TCCAAGTAGT 
GAAAGAACTA CACCTGCCTT TCATCCTGTA TGTCAGGTGA TTGCTATGTA TGACTATGTA 
GCAAATAACG AGGATGAGCT GAATTTCTCC AAGGGACAAC TTATCAATGT TTTGAACAAA 
GATGATCCTG ACTGGTGGCA AGGAGAAATC AACGGGGTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCCGATCCA AGTCAACAGT GGTGTGCTGA TCTCCAAAGC 
CTGGACACCA TGCAGCCAGT TGAGAGGAAG AGGCAGGGCT ATATTCACGA GCTGATTCAG 
ACCGAAGAGC GGTACGTGGA CGACCTTCAG CTCGTCGTGG AGGTTTTTCA GAAACGGATG 
GTGGAGTCGG GCTTTCTCAG TGAAGGAGAA ATGGCCTTGA TTTTTGTAAA CTGGAAAGAA 
CTCATCATGT CTAACACAAA GCTACTCAAG GCCTTGCGGG TACGGAAGAA GACCGGGGGA 
GAGAAGATGC CCGTGCAGAT GATTGGGGAC ATCCTGGCAG CCGAGCTGTC CCACATGCAG 
GCTTATATCC GGTTCTGTAG CTGTCAGCTT AATGGAGCAG CTCTGTTACA GCAGAAGACC 
GATGAAGACA CGGATTTCAA GGAATTTTTA AAGAAGCTGG CATCTGACCC TCGATGTAAA 
GGAATGCCCC TTTCCAGCTT CCTGCTGAAG CCCATGCAGA GAATCACCCG CTACCCTCTG 
CTCATCAGAA GTATTCTGGA GAACACCCTG GAGAGTCATG TGGACCATTC CTCCCTAAAG 
CTGGCTCTTG AGCGGGCAGA GGAGCTTTGT TCCCAAGTTA ATGAAGGAGT TCGGGAAAAA 
GAAAATTCAG ACCGACTCGA GTGGATCCAG GCACACGTTC AGTGTGAAGG TCTTGCAGAG 
CAACTTATTT TCAACTCCCT CACCAACTGC CTGGGGCCCC GGAAGCTTCT GCACAGTGGG 
AAACTATACA AGACCAAGAG CAACAAGGAA CTACACGGAT TCCTCTTCAA TGACTTTCTG 
CTTCTTACCT ACATGGTCAA GCAGTTTGCT GTTTCCTCAG GTTCTGAGAA GCTTTTCAGC 
TCTAAGTCCA ATGCCCAATT CAAAATGTAT AAAACGCCCA TTTTCCTGAA TGAAGTCCTG 
GTGAAACTGC CCACAGACCC TTCCAGCGAT GAGCCTGTCT TCCACATTTC TCACATTGAC 
CGGGTCTACA CCCTCCGAAC AGACAACATT AACGAGAGGA CTGCTTGGGT CCAGAAGATC 
AAGGCGGCAT CTGAGCAGTA CATTGACACG GAAAAGAAGA AACGGGAGAA GGCTTATCAA 
GCTCGCTCTC AAAAGACTTC AGGCATTGGG CGTCTGATGG TGCACGTCAT CGAAGCTACA 
GAATTAAAAG CCTGTAAACC GAATGGAAAG AGCAATCCAT ACTGTGAAAT CAGCATGGGC 
TCCCAGAGCT ATACCACCAG AACCCTCCAG GACACACTCA ATCCCAAGTG GAACTTTAAC 
TGCCAGTTCT TTATTAAGGA CCTTTACCAA GATGTGCTGT GTCTCACCAT GTTTGACAGA 
GACCAGTTTT CACCAGATGA TTTCCTGGGT CGCACTGAAG TTCCAGTGGC AAAAATTCGC 
ACAGAACAGG AAAGCAAAGG CCCCACAACC CGCCGACTCC TGCTGCACGA GGTCCCCACC 
GGGGAGGTCT GGGTCCGCTT TGACCTGCAG CTTTTTGAGC AAAAAACTCT CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827839.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683398.1 Felis catus intersectin 2 (ITSN2), transcript variant X9, mRNA.

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CloneID	OFb32215
Clone ID Related Accession (Same CDS sequence)	XM_019827839.1
Accession Version	XM_019827839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA ACTTTATAAG 
ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAGAAG 
AAACTAGAAG ATGAGGCTGT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGAGAA 
AACCTTAGAA AAGAGGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AACACAAGAA 
AAAGTTCAAG AAGAGGAACG GAAGGCTGAG GAGAAACCAT GTGAGCCAGC TACTGCTTTG 
GTGAATTATA GAGCATTGTA CCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT 
TCCGGGGATA TAATACAGGT TGATGAAAAA ACCGTAGGAG AACCTGGTTG GCTTTATGGT 
AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCCATCAGGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCAGT ATCTGCTACC 
TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT 
ACTGTTTCCC CTGGATCTGT ATCACCGATT CATGGCCAGG GACAGGTTGT AGAAAACCTA 
AAGGCGCAGG CCCTGTGTTC TTGGACTGCA AAGAAGGATA ACCACTTGAA CTTTTCAAAA 
CATGATATTA TCACTGTGTT GGAACAGCAA GAAAATTGGT GGTTTGGAGA GGTGCATGGA 
GGAAGAGGGT GGTTTCCAAA ATCTTATGTC AAAATACTCC CTGGGAGTGA AGTCAAGCGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTACACA 
GGTGGAGAGG AATATATTGC ACTTTATCCA TATTCAAGTG TAGAACCTGG AGATTTGACT 
TTCACTGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAATGGTG GACAGGAAGT 
ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAATC TGGAACATCA AACAAGAAAC CTGAGATTGC TCAAGTAACT 
TCAGCATATG TGGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCCGGGCA GTTAATACTA 
ATCCTAAAGA AAAATTCAAG TGGGTGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
ACTACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGTAGCAAAT 
AACGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT 
CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT 
AAGATGACAA CAGACTCCGA TCCAAGTCAA CAGTGGTGTG CTGATCTCCA AAGCCTGGAC 
ACCATGCAGC CAGTTGAGAG GAAGAGGCAG GGCTATATTC ACGAGCTGAT TCAGACCGAA 
GAGCGGTACG TGGACGACCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGTGGAG 
TCGGGCTTTC TCAGTGAAGG AGAAATGGCC TTGATTTTTG TAAACTGGAA AGAACTCATC 
ATGTCTAACA CAAAGCTACT CAAGGCCTTG CGGGTACGGA AGAAGACCGG GGGAGAGAAG 
ATGCCCGTGC AGATGATTGG GGACATCCTG GCAGCCGAGC TGTCCCACAT GCAGGCTTAT 
ATCCGGTTCT GTAGCTGTCA GCTTAATGGA GCAGCTCTGT TACAGCAGAA GACCGATGAA 
GACACGGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGATG TAAAGGAATG 
CCCCTTTCCA GCTTCCTGCT GAAGCCCATG CAGAGAATCA CCCGCTACCC TCTGCTCATC 
AGAAGTATTC TGGAGAACAC CCTGGAGAGT CATGTGGACC ATTCCTCCCT AAAGCTGGCT 
CTTGAGCGGG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT 
TCAGACCGAC TCGAGTGGAT CCAGGCACAC GTTCAGTGTG AAGGTCTTGC AGAGCAACTT 
ATTTTCAACT CCCTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAACTA 
TACAAGACCA AGAGCAACAA GGAACTACAC GGATTCCTCT TCAATGACTT TCTGCTTCTT 
ACCTACATGG TCAAGCAGTT TGCTGTTTCC TCAGGTTCTG AGAAGCTTTT CAGCTCTAAG 
TCCAATGCCC AATTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CCTGGTGAAA 
CTGCCCACAG ACCCTTCCAG CGATGAGCCT GTCTTCCACA TTTCTCACAT TGACCGGGTC 
TACACCCTCC GAACAGACAA CATTAACGAG AGGACTGCTT GGGTCCAGAA GATCAAGGCG 
GCATCTGAGC AGTACATTGA CACGGAAAAG AAGAAACGGG AGAAGGCTTA TCAAGCTCGC 
TCTCAAAAGA CTTCAGGCAT TGGGCGTCTG ATGGTGCACG TCATCGAAGC TACAGAATTA 
AAAGCCTGTA AACCGAATGG AAAGAGCAAT CCATACTGTG AAATCAGCAT GGGCTCCCAG 
AGCTATACCA CCAGAACCCT CCAGGACACA CTCAATCCCA AGTGGAACTT TAACTGCCAG 
TTCTTTATTA AGGACCTTTA CCAAGATGTG CTGTGTCTCA CCATGTTTGA CAGAGACCAG 
TTTTCACCAG ATGATTTCCT GGGTCGCACT GAAGTTCCAG TGGCAAAAAT TCGCACAGAA 
CAGGAAAGCA AAGGCCCCAC AACCCGCCGA CTCCTGCTGC ACGAGGTCCC CACCGGGGAG 
GTCTGGGTCC GCTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683398.1
CDS	381..5351
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827839.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827839.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA ACTTTATAAG 
ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAGAAG 
AAACTAGAAG ATGAGGCTGT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGAGAA 
AACCTTAGAA AAGAGGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AACACAAGAA 
AAAGTTCAAG AAGAGGAACG GAAGGCTGAG GAGAAACCAT GTGAGCCAGC TACTGCTTTG 
GTGAATTATA GAGCATTGTA CCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT 
TCCGGGGATA TAATACAGGT TGATGAAAAA ACCGTAGGAG AACCTGGTTG GCTTTATGGT 
AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCCATCAGGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCAGT ATCTGCTACC 
TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT 
ACTGTTTCCC CTGGATCTGT ATCACCGATT CATGGCCAGG GACAGGTTGT AGAAAACCTA 
AAGGCGCAGG CCCTGTGTTC TTGGACTGCA AAGAAGGATA ACCACTTGAA CTTTTCAAAA 
CATGATATTA TCACTGTGTT GGAACAGCAA GAAAATTGGT GGTTTGGAGA GGTGCATGGA 
GGAAGAGGGT GGTTTCCAAA ATCTTATGTC AAAATACTCC CTGGGAGTGA AGTCAAGCGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTACACA 
GGTGGAGAGG AATATATTGC ACTTTATCCA TATTCAAGTG TAGAACCTGG AGATTTGACT 
TTCACTGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAATGGTG GACAGGAAGT 
ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAATC TGGAACATCA AACAAGAAAC CTGAGATTGC TCAAGTAACT 
TCAGCATATG TGGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCCGGGCA GTTAATACTA 
ATCCTAAAGA AAAATTCAAG TGGGTGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
ACTACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGTAGCAAAT 
AACGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT 
CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT 
AAGATGACAA CAGACTCCGA TCCAAGTCAA CAGTGGTGTG CTGATCTCCA AAGCCTGGAC 
ACCATGCAGC CAGTTGAGAG GAAGAGGCAG GGCTATATTC ACGAGCTGAT TCAGACCGAA 
GAGCGGTACG TGGACGACCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGTGGAG 
TCGGGCTTTC TCAGTGAAGG AGAAATGGCC TTGATTTTTG TAAACTGGAA AGAACTCATC 
ATGTCTAACA CAAAGCTACT CAAGGCCTTG CGGGTACGGA AGAAGACCGG GGGAGAGAAG 
ATGCCCGTGC AGATGATTGG GGACATCCTG GCAGCCGAGC TGTCCCACAT GCAGGCTTAT 
ATCCGGTTCT GTAGCTGTCA GCTTAATGGA GCAGCTCTGT TACAGCAGAA GACCGATGAA 
GACACGGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGATG TAAAGGAATG 
CCCCTTTCCA GCTTCCTGCT GAAGCCCATG CAGAGAATCA CCCGCTACCC TCTGCTCATC 
AGAAGTATTC TGGAGAACAC CCTGGAGAGT CATGTGGACC ATTCCTCCCT AAAGCTGGCT 
CTTGAGCGGG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT 
TCAGACCGAC TCGAGTGGAT CCAGGCACAC GTTCAGTGTG AAGGTCTTGC AGAGCAACTT 
ATTTTCAACT CCCTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAACTA 
TACAAGACCA AGAGCAACAA GGAACTACAC GGATTCCTCT TCAATGACTT TCTGCTTCTT 
ACCTACATGG TCAAGCAGTT TGCTGTTTCC TCAGGTTCTG AGAAGCTTTT CAGCTCTAAG 
TCCAATGCCC AATTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CCTGGTGAAA 
CTGCCCACAG ACCCTTCCAG CGATGAGCCT GTCTTCCACA TTTCTCACAT TGACCGGGTC 
TACACCCTCC GAACAGACAA CATTAACGAG AGGACTGCTT GGGTCCAGAA GATCAAGGCG 
GCATCTGAGC AGTACATTGA CACGGAAAAG AAGAAACGGG AGAAGGCTTA TCAAGCTCGC 
TCTCAAAAGA CTTCAGGCAT TGGGCGTCTG ATGGTGCACG TCATCGAAGC TACAGAATTA 
AAAGCCTGTA AACCGAATGG AAAGAGCAAT CCATACTGTG AAATCAGCAT GGGCTCCCAG 
AGCTATACCA CCAGAACCCT CCAGGACACA CTCAATCCCA AGTGGAACTT TAACTGCCAG 
TTCTTTATTA AGGACCTTTA CCAAGATGTG CTGTGTCTCA CCATGTTTGA CAGAGACCAG 
TTTTCACCAG ATGATTTCCT GGGTCGCACT GAAGTTCCAG TGGCAAAAAT TCGCACAGAA 
CAGGAAAGCA AAGGCCCCAC AACCCGCCGA CTCCTGCTGC ACGAGGTCCC CACCGGGGAG 
GTCTGGGTCC GCTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_019827835.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683394.1 Felis catus intersectin 2 (ITSN2), transcript variant X5, mRNA.

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CloneID	OFb32216
Clone ID Related Accession (Same CDS sequence)	XM_019827835.1
Accession Version	XM_019827835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	intersectin-2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTAAGGGTG AGCCAGCTAC TGCTTTGGTG AATTATAGAG CATTGTACCG CTTTGAAGCC 
AGGAGCCATG ATGAGATGAG TTTTAATTCC GGGGATATAA TACAGGTTGA TGAAAAAACC 
GTAGGAGAAC CTGGTTGGCT TTATGGTAGT TTTCAAGGAA ATTTTGGCTG GTTTCCTTGC 
AACTATGTAG AAAAAATGCC ATCAGGTGAA AAATCTGTGT CTCCTAAGAA GGCCTTACTT 
CCCCCTACAG TATCAGTATC TGCTACCTCA ACTTCTTCTG AATCACTTTC TTCAAATCAA 
CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTACAA ACCTAACTGT TAATACATCA 
TGGCAGAAAA AATCAGCTTT TACTCGTACT GTTTCCCCTG GATCTGTATC ACCGATTCAT 
GGCCAGGGAC AGGTTGTAGA AAACCTAAAG GCGCAGGCCC TGTGTTCTTG GACTGCAAAG 
AAGGATAACC ACTTGAACTT TTCAAAACAT GATATTATCA CTGTGTTGGA ACAGCAAGAA 
AATTGGTGGT TTGGAGAGGT GCATGGAGGA AGAGGGTGGT TTCCAAAATC TTATGTCAAA 
ATACTCCCTG GGAGTGAAGT CAAGCGGGAA GAACCAGAAG CTTTGTATGC AGCTGTAAAC 
AAGAAACCTA CCTCTGCAGC CTACACAGGT GGAGAGGAAT ATATTGCACT TTATCCATAT 
TCAAGTGTAG AACCTGGAGA TTTGACTTTC ACTGAAGGTG AAGAAATATT GGTGACCCAG 
AAAGATGGAG AATGGTGGAC AGGAAGTATT GGAGATAGAA CTGGAATTTT TCCATCAAAT 
TATGTCAAAC CAAAAGATCA AGAGAGTTTT GGGAGTGCTA GCAAATCTGG AACATCAAAC 
AAGAAACCTG AGATTGCTCA AGTAACTTCA GCATATGTGG CTTCTGGTTC TGAACAACTT 
AGCCTTGCAC CCGGGCAGTT AATACTAATC CTAAAGAAAA ATTCAAGTGG GTGGTGGCAA 
GGGGAATTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT GGTTTCCTGC CAGCCATGTT 
AAACTTTTGG GTCCAAGTAG TGAAAGAACT ACACCTGCCT TTCATCCTGT ATGTCAGGTG 
ATTGCTATGT ATGACTATGT AGCAAATAAC GAGGATGAGC TGAATTTCTC CAAGGGACAA 
CTTATCAATG TTTTGAACAA AGATGATCCT GACTGGTGGC AAGGAGAAAT CAACGGGGTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCCGATCC AAGTCAACAG 
TGGTGTGCTG ATCTCCAAAG CCTGGACACC ATGCAGCCAG TTGAGAGGAA GAGGCAGGGC 
TATATTCACG AGCTGATTCA GACCGAAGAG CGGTACGTGG ACGACCTTCA GCTCGTCGTG 
GAGGTTTTTC AGAAACGGAT GGTGGAGTCG GGCTTTCTCA GTGAAGGAGA AATGGCCTTG 
ATTTTTGTAA ACTGGAAAGA ACTCATCATG TCTAACACAA AGCTACTCAA GGCCTTGCGG 
GTACGGAAGA AGACCGGGGG AGAGAAGATG CCCGTGCAGA TGATTGGGGA CATCCTGGCA 
GCCGAGCTGT CCCACATGCA GGCTTATATC CGGTTCTGTA GCTGTCAGCT TAATGGAGCA 
GCTCTGTTAC AGCAGAAGAC CGATGAAGAC ACGGATTTCA AGGAATTTTT AAAGAAGCTG 
GCATCTGACC CTCGATGTAA AGGAATGCCC CTTTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGAATCACCC GCTACCCTCT GCTCATCAGA AGTATTCTGG AGAACACCCT GGAGAGTCAT 
GTGGACCATT CCTCCCTAAA GCTGGCTCTT GAGCGGGCAG AGGAGCTTTG TTCCCAAGTT 
AATGAAGGAG TTCGGGAAAA AGAAAATTCA GACCGACTCG AGTGGATCCA GGCACACGTT 
CAGTGTGAAG GTCTTGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGGCCC 
CGGAAGCTTC TGCACAGTGG GAAACTATAC AAGACCAAGA GCAACAAGGA ACTACACGGA 
TTCCTCTTCA ATGACTTTCT GCTTCTTACC TACATGGTCA AGCAGTTTGC TGTTTCCTCA 
GGTTCTGAGA AGCTTTTCAG CTCTAAGTCC AATGCCCAAT TCAAAATGTA TAAAACGCCC 
ATTTTCCTGA ATGAAGTCCT GGTGAAACTG CCCACAGACC CTTCCAGCGA TGAGCCTGTC 
TTCCACATTT CTCACATTGA CCGGGTCTAC ACCCTCCGAA CAGACAACAT TAACGAGAGG 
ACTGCTTGGG TCCAGAAGAT CAAGGCGGCA TCTGAGCAGT ACATTGACAC GGAAAAGAAG 
AAACGGGAGA AGGCTTATCA AGCTCGCTCT CAAAAGACTT CAGGCATTGG GCGTCTGATG 
GTGCACGTCA TCGAAGCTAC AGAATTAAAA GCCTGTAAAC CGAATGGAAA GAGCAATCCA 
TACTGTGAAA TCAGCATGGG CTCCCAGAGC TATACCACCA GAACCCTCCA GGACACACTC 
AATCCCAAGT GGAACTTTAA CTGCCAGTTC TTTATTAAGG ACCTTTACCA AGATGTGCTG 
TGTCTCACCA TGTTTGACAG AGACCAGTTT TCACCAGATG ATTTCCTGGG TCGCACTGAA 
GTTCCAGTGG CAAAAATTCG CACAGAACAG GAAAGCAAAG GCCCCACAAC CCGCCGACTC 
CTGCTGCACG AGGTCCCCAC CGGGGAGGTC TGGGTCCGCT TTGACCTGCA GCTTTTTGAG 
CAAAAAACTC TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683394.1
CDS	91..5148
Translation	MMAQFPTTMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPHSLPPVAPMATPLPSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGGASLQKAQSLIDLGSSSSTSSTASLSGNSPKTGTLEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLYKIKRDKLKEIERKRSELIQKKKLEDEAVRKAKQGKENLWRENLRKEEEEKQKRLQEEKTQEKVQEEERKAEEKPCKGEPATALVNYRALYRFEARSHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVSFTNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKILPGSEVKREEPEALYAAVNKKPTSAAYTGGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQESFGSASKSGTSNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYVANNEDELNFSKGQLINVLNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPVERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLSEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLESHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_019827835.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus intersectin 2 (ITSN2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827835.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AACTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCCTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA AGAATTCTCC 
ATAGCGATGA AGCTCATCAA ACTGAAGCTA CAAGGCCAGC AGCTGCCCGT GGTCCTCCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GGCAACCCCC 
TTACCCTCTG CGACTTCAGG GACCACCCTT CCTCCCTTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACATCGTC ACTACCAAAT GGAACCGCTG GCCTCATTCA GCCGTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTCAT GATGGGAGGA 
CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCACT CTCTGGAAAC TCGCCCAAGA CTGGGACCTT AGAGTGGGCG 
GTTCCTCAGC CTTCAAGGTT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG 
AGTGGATATC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATTTG GACCCTTGCT GACATTGATG GCGACGGACA GCTGAAAGCT 
GAAGAGTTCA TCCTTGCGAT GCATCTCACT GACATGGCCA AAGCTGGACA GCCCTTGCCA 
CTGACGTTGC CTCCTGAACT TGTCCCTCCA TCTTTCAGAG GAGGAAAGCA AATTGATTCC 
ATTAATGGAA CTCTGCCTTC CTATCAGAAA ATTCAAGAAG AGGAACCTCA GAAAAAATTA 
CCAGTTACTT TCGAAGACAA GCGGAAGGCC AACTACGAGC GAGGGAACGT GGAACTGGAG 
AAGCGGCGCC AAGCCCTGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGAAA AGCCCAGAAA 
GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AACAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGAGACAGCG GGAGGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGGATTCG TCGCCAGGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TCGAGTTGGA AGCACTGAAT 
GGCAAACACC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA 
AAGACTGAGC TGGAAGTTCT AGATAAACAG TGTGACCTGG AAATTATGGA AATCAAGCAA 
CTCCAACAAG AACTTCAGGA TTATCAAAAT AAACTTATCT ATTTGGTACC GGAGAAGCAA 
TTATTAAATG AAAGAATTAA GAACATGCAG CTTGGTAACA CACCTGATTT AGGGATTGGT 
TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAATCAA 
TTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTTATAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG 
TCAGAGCTAA TACAGAAGAA GAAACTAGAA GATGAGGCTG TAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TATGGAGAGA AAACCTTAGA AAAGAGGAAG AAGAAAAACA AAAGCGACTC 
CAGGAAGAAA AAACACAAGA AAAAGTTCAA GAAGAGGAAC GGAAGGCTGA GGAGAAACCA 
TGTAAGGGTG AGCCAGCTAC TGCTTTGGTG AATTATAGAG CATTGTACCG CTTTGAAGCC 
AGGAGCCATG ATGAGATGAG TTTTAATTCC GGGGATATAA TACAGGTTGA TGAAAAAACC 
GTAGGAGAAC CTGGTTGGCT TTATGGTAGT TTTCAAGGAA ATTTTGGCTG GTTTCCTTGC 
AACTATGTAG AAAAAATGCC ATCAGGTGAA AAATCTGTGT CTCCTAAGAA GGCCTTACTT 
CCCCCTACAG TATCAGTATC TGCTACCTCA ACTTCTTCTG AATCACTTTC TTCAAATCAA 
CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTACAA ACCTAACTGT TAATACATCA 
TGGCAGAAAA AATCAGCTTT TACTCGTACT GTTTCCCCTG GATCTGTATC ACCGATTCAT 
GGCCAGGGAC AGGTTGTAGA AAACCTAAAG GCGCAGGCCC TGTGTTCTTG GACTGCAAAG 
AAGGATAACC ACTTGAACTT TTCAAAACAT GATATTATCA CTGTGTTGGA ACAGCAAGAA 
AATTGGTGGT TTGGAGAGGT GCATGGAGGA AGAGGGTGGT TTCCAAAATC TTATGTCAAA 
ATACTCCCTG GGAGTGAAGT CAAGCGGGAA GAACCAGAAG CTTTGTATGC AGCTGTAAAC 
AAGAAACCTA CCTCTGCAGC CTACACAGGT GGAGAGGAAT ATATTGCACT TTATCCATAT 
TCAAGTGTAG AACCTGGAGA TTTGACTTTC ACTGAAGGTG AAGAAATATT GGTGACCCAG 
AAAGATGGAG AATGGTGGAC AGGAAGTATT GGAGATAGAA CTGGAATTTT TCCATCAAAT 
TATGTCAAAC CAAAAGATCA AGAGAGTTTT GGGAGTGCTA GCAAATCTGG AACATCAAAC 
AAGAAACCTG AGATTGCTCA AGTAACTTCA GCATATGTGG CTTCTGGTTC TGAACAACTT 
AGCCTTGCAC CCGGGCAGTT AATACTAATC CTAAAGAAAA ATTCAAGTGG GTGGTGGCAA 
GGGGAATTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT GGTTTCCTGC CAGCCATGTT 
AAACTTTTGG GTCCAAGTAG TGAAAGAACT ACACCTGCCT TTCATCCTGT ATGTCAGGTG 
ATTGCTATGT ATGACTATGT AGCAAATAAC GAGGATGAGC TGAATTTCTC CAAGGGACAA 
CTTATCAATG TTTTGAACAA AGATGATCCT GACTGGTGGC AAGGAGAAAT CAACGGGGTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCCGATCC AAGTCAACAG 
TGGTGTGCTG ATCTCCAAAG CCTGGACACC ATGCAGCCAG TTGAGAGGAA GAGGCAGGGC 
TATATTCACG AGCTGATTCA GACCGAAGAG CGGTACGTGG ACGACCTTCA GCTCGTCGTG 
GAGGTTTTTC AGAAACGGAT GGTGGAGTCG GGCTTTCTCA GTGAAGGAGA AATGGCCTTG 
ATTTTTGTAA ACTGGAAAGA ACTCATCATG TCTAACACAA AGCTACTCAA GGCCTTGCGG 
GTACGGAAGA AGACCGGGGG AGAGAAGATG CCCGTGCAGA TGATTGGGGA CATCCTGGCA 
GCCGAGCTGT CCCACATGCA GGCTTATATC CGGTTCTGTA GCTGTCAGCT TAATGGAGCA 
GCTCTGTTAC AGCAGAAGAC CGATGAAGAC ACGGATTTCA AGGAATTTTT AAAGAAGCTG 
GCATCTGACC CTCGATGTAA AGGAATGCCC CTTTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGAATCACCC GCTACCCTCT GCTCATCAGA AGTATTCTGG AGAACACCCT GGAGAGTCAT 
GTGGACCATT CCTCCCTAAA GCTGGCTCTT GAGCGGGCAG AGGAGCTTTG TTCCCAAGTT 
AATGAAGGAG TTCGGGAAAA AGAAAATTCA GACCGACTCG AGTGGATCCA GGCACACGTT 
CAGTGTGAAG GTCTTGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGGCCC 
CGGAAGCTTC TGCACAGTGG GAAACTATAC AAGACCAAGA GCAACAAGGA ACTACACGGA 
TTCCTCTTCA ATGACTTTCT GCTTCTTACC TACATGGTCA AGCAGTTTGC TGTTTCCTCA 
GGTTCTGAGA AGCTTTTCAG CTCTAAGTCC AATGCCCAAT TCAAAATGTA TAAAACGCCC 
ATTTTCCTGA ATGAAGTCCT GGTGAAACTG CCCACAGACC CTTCCAGCGA TGAGCCTGTC 
TTCCACATTT CTCACATTGA CCGGGTCTAC ACCCTCCGAA CAGACAACAT TAACGAGAGG 
ACTGCTTGGG TCCAGAAGAT CAAGGCGGCA TCTGAGCAGT ACATTGACAC GGAAAAGAAG 
AAACGGGAGA AGGCTTATCA AGCTCGCTCT CAAAAGACTT CAGGCATTGG GCGTCTGATG 
GTGCACGTCA TCGAAGCTAC AGAATTAAAA GCCTGTAAAC CGAATGGAAA GAGCAATCCA 
TACTGTGAAA TCAGCATGGG CTCCCAGAGC TATACCACCA GAACCCTCCA GGACACACTC 
AATCCCAAGT GGAACTTTAA CTGCCAGTTC TTTATTAAGG ACCTTTACCA AGATGTGCTG 
TGTCTCACCA TGTTTGACAG AGACCAGTTT TCACCAGATG ATTTCCTGGG TCGCACTGAA 
GTTCCAGTGG CAAAAATTCG CACAGAACAG GAAAGCAAAG GCCCCACAAC CCGCCGACTC 
CTGCTGCACG AGGTCCCCAC CGGGGAGGTC TGGGTCCGCT TTGACCTGCA GCTTTTTGAG 
CAAAAAACTC TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ITSN2
Entrez Gene ID	101095168
Full Name	intersectin 2
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ITSN2 cDNA ORF clone, Felis catus(domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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