NRT1-2 cDNA ORF clone, Glycine max(soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRT1-2
Entrez Gene ID	100101839
Full Name	nitrate transporter NRT1-2
Synonyms	NRT1-1
Gene Type	protein-coding
Organism	Glycine max(soybean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001251041.2	NP_001237970.2	nitrate transporter NRT1-2

Pathways from BioSystems
Gene Ontology

The following NRT1-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRT1-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGl60000	NM_001251041.2 Latest version!	Glycine max nitrate transporter NRT1-2 (NRT1-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRT1-2 ( NM_001251041.2 ) cDNA ORF clone, Glycine max(soybean) -> NP_001237970.2 Glycine max nitrate transporter NRT1-2 (NRT1-2), mRNA.

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CloneID	OGl60000
Clone ID Related Accession (Same CDS sequence)	NM_001251041.2
Accession Version	NM_001251041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-18
Organism	Glycine max(soybean)
Product	nitrate transporter NRT1-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB052785.1 and ACUP03010362.1. On Mar 9, 2017 this sequence version replaced NM_001251041.1. ##Evidence-Data-START## Transcript exon combination :: AB052785.1, AB052784.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00264988, SAMN00769648 [ECO:0000348] ##Evidence-Data-END##

ATGACAGGCG TAGAGACAAT GGGAAAAAAT GAGAATCAAA GTGTGGAAAA AAATGAGAAA 
AGTGTCATAG ATGAGGAGCC AAAGATTAAC TACAGAGGAT GGAAAGTCAT GCCCTTTATC 
ATTGGGAATG AAACTTTTGA GAAGCTAGGA ACCATTGGAA CCTTAGCCAA CCTCTTGGTC 
TATCTCACAA CCGTCTTCAA CTTGAGCAGT CTTACAGCTA CAAATATTAT TAACATCTTC 
ACTGGCAGCG CCAGTTTATC TACCTTGCTT GGGGCTTTTC TTTGTGACAC TTATTTTGGC 
CGCTACAAGA CACTGGGATT CTGCACAATT GCTTCTTTTC TGGGGTTGCT TGTTATACAA 
CTAACAGCAT GGATTAAGAA AATGCATCCA CCTCACTGTG GAAGTGAGAG CATCACATGC 
ACAGGCCCAA CCACAGGGCA AATGACATTT CTGCTTGCTG GATTTGGACT TCTAATTGTG 
GGAGCTGCAG GGATCAGACC TTGTAACTTA GCATTTGGAG TTGATCAGTT CAACCCCAAC 
ACTGAGTCTG GAAAAAAGGG AATCAACAGC TTCTTCAATT GGTACTTCTT TACCTACACA 
TTTGCTCAAA TGGTGTCTTT GTCACTAATT GTCTACATAC AGTCAAACGT TAGCTGGGCA 
ATAGGGTTGG GAATTCCTGC GGCTTTGATG CTAATCTCTT GCACACTCTA CTACACGGGA 
AGCAATTATT ACGTGAAAGT TAAAGCAACT GGTCCAGCTC CACTAACAAG TTTAGCTCAA 
GCTGTAGTTG TGGCTATAAA GAAAAGAAGA CTGAATCTAT CAGAATATCC ACTGGATTCT 
TCGCTCTTTG CCTATGTTTC TCCCCAGTCT ATTAACTCCA AGCTTCTTCA CACGTCTCAG 
TTCAGGTTCC TAGACAAAGC TGCAATTATA ACCCCACAAG ACGGCATAAA CCCAGACGGG 
TCTGCATCAG ATCCTTGGAG TCTTTGCAGC ATGCAACAAG TGGAAGAGCT AAAATGCTTG 
CTGAGAGTGA TCCCCATTTG GTTCGCAGGA ATTTTCTTCT ACATTGCAAT AGTCCAACAG 
AACACAATGC TGGTCTTCCA AGCCCTTCAA TCCGACAGAC GCATTCTCAG CACCAACTTC 
AAGATCCTAG CAGCCTCCTA CACCATCTTC CAGATGCTGA GCCTAACCAT ATGGCTACCC 
ATCTACGACC GAATCCTCGT GCCGTCGCTC CAAAGGGTCA CCAAAAAAGA GGGTGGCATC 
ACAGTTCTTC AAAGAATAGG CTTTGGCATG TTCCTCTCTA TACTATGCAC AATGGTGTCT 
GGTGTAGTGG AAGAAAGAAG AAGGACATTG GCTTTGACCA ATCCTATTGG ATTAGAGCCA 
AGAAAAGGTG CCATTTCTTC AATGTCAGGT CTATGGTTGG TTCCTCAGCT AACACTGGCA 
GGGTTATCTG ATGCATTCGC GATTGTGGGA CAAGTTGAGT TTTTCTACAA ACAGTTTCCA 
GAGAACATGA AAAGCCTTGC AGCGTCTCTA TTTTTCTGTG GCCTCGCGGG ATCAAGTTAT 
TTGAGTAGTT TGCTGATTTC TATTATCCAT AGGGCGACAG CTAAATCTTC AACTGGGAAT 
TGGTTACCTC AGGATCTCAA TAAGGGGAGA TTGGATTATT TTTATTACAT TATAACTGCT 
CTAGAAGTCG TTAATTTCGG TTACTTTATA TTGTGTGCCA AGTGGTATAA ATACAAAGGG 
ACTGGTTCTA GCAGCAGTGG TGACCTTCAA CTCGATCAAG TATCCAAACC ATCCGAAAGA 
ACTGTTAACA CTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001237970.2
CDS	136..1953
Translation	MTGVETMGKNENQSVEKNEKSVIDEEPKINYRGWKVMPFIIGNETFEKLGTIGTLANLLVYLTTVFNLSSLTATNIINIFTGSASLSTLLGAFLCDTYFGRYKTLGFCTIASFLGLLVIQLTAWIKKMHPPHCGSESITCTGPTTGQMTFLLAGFGLLIVGAAGIRPCNLAFGVDQFNPNTESGKKGINSFFNWYFFTYTFAQMVSLSLIVYIQSNVSWAIGLGIPAALMLISCTLYYTGSNYYVKVKATGPAPLTSLAQAVVVAIKKRRLNLSEYPLDSSLFAYVSPQSINSKLLHTSQFRFLDKAAIITPQDGINPDGSASDPWSLCSMQQVEELKCLLRVIPIWFAGIFFYIAIVQQNTMLVFQALQSDRRILSTNFKILAASYTIFQMLSLTIWLPIYDRILVPSLQRVTKKEGGITVLQRIGFGMFLSILCTMVSGVVEERRRTLALTNPIGLEPRKGAISSMSGLWLVPQLTLAGLSDAFAIVGQVEFFYKQFPENMKSLAASLFFCGLAGSSYLSSLLISIIHRATAKSSTGNWLPQDLNKGRLDYFYYIITALEVVNFGYFILCAKWYKYKGTGSSSSGDLQLDQVSKPSERTVNTV

Target ORF information:

RefSeq Version	NM_001251041.2
Organism	Glycine max(soybean)
Definition	Glycine max nitrate transporter NRT1-2 (NRT1-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251041.2

ORF Insert Sequence:

ATGACAGGCG TAGAGACAAT GGGAAAAAAT GAGAATCAAA GTGTGGAAAA AAATGAGAAA 
AGTGTCATAG ATGAGGAGCC AAAGATTAAC TACAGAGGAT GGAAAGTCAT GCCCTTTATC 
ATTGGGAATG AAACTTTTGA GAAGCTAGGA ACCATTGGAA CCTTAGCCAA CCTCTTGGTC 
TATCTCACAA CCGTCTTCAA CTTGAGCAGT CTTACAGCTA CAAATATTAT TAACATCTTC 
ACTGGCAGCG CCAGTTTATC TACCTTGCTT GGGGCTTTTC TTTGTGACAC TTATTTTGGC 
CGCTACAAGA CACTGGGATT CTGCACAATT GCTTCTTTTC TGGGGTTGCT TGTTATACAA 
CTAACAGCAT GGATTAAGAA AATGCATCCA CCTCACTGTG GAAGTGAGAG CATCACATGC 
ACAGGCCCAA CCACAGGGCA AATGACATTT CTGCTTGCTG GATTTGGACT TCTAATTGTG 
GGAGCTGCAG GGATCAGACC TTGTAACTTA GCATTTGGAG TTGATCAGTT CAACCCCAAC 
ACTGAGTCTG GAAAAAAGGG AATCAACAGC TTCTTCAATT GGTACTTCTT TACCTACACA 
TTTGCTCAAA TGGTGTCTTT GTCACTAATT GTCTACATAC AGTCAAACGT TAGCTGGGCA 
ATAGGGTTGG GAATTCCTGC GGCTTTGATG CTAATCTCTT GCACACTCTA CTACACGGGA 
AGCAATTATT ACGTGAAAGT TAAAGCAACT GGTCCAGCTC CACTAACAAG TTTAGCTCAA 
GCTGTAGTTG TGGCTATAAA GAAAAGAAGA CTGAATCTAT CAGAATATCC ACTGGATTCT 
TCGCTCTTTG CCTATGTTTC TCCCCAGTCT ATTAACTCCA AGCTTCTTCA CACGTCTCAG 
TTCAGGTTCC TAGACAAAGC TGCAATTATA ACCCCACAAG ACGGCATAAA CCCAGACGGG 
TCTGCATCAG ATCCTTGGAG TCTTTGCAGC ATGCAACAAG TGGAAGAGCT AAAATGCTTG 
CTGAGAGTGA TCCCCATTTG GTTCGCAGGA ATTTTCTTCT ACATTGCAAT AGTCCAACAG 
AACACAATGC TGGTCTTCCA AGCCCTTCAA TCCGACAGAC GCATTCTCAG CACCAACTTC 
AAGATCCTAG CAGCCTCCTA CACCATCTTC CAGATGCTGA GCCTAACCAT ATGGCTACCC 
ATCTACGACC GAATCCTCGT GCCGTCGCTC CAAAGGGTCA CCAAAAAAGA GGGTGGCATC 
ACAGTTCTTC AAAGAATAGG CTTTGGCATG TTCCTCTCTA TACTATGCAC AATGGTGTCT 
GGTGTAGTGG AAGAAAGAAG AAGGACATTG GCTTTGACCA ATCCTATTGG ATTAGAGCCA 
AGAAAAGGTG CCATTTCTTC AATGTCAGGT CTATGGTTGG TTCCTCAGCT AACACTGGCA 
GGGTTATCTG ATGCATTCGC GATTGTGGGA CAAGTTGAGT TTTTCTACAA ACAGTTTCCA 
GAGAACATGA AAAGCCTTGC AGCGTCTCTA TTTTTCTGTG GCCTCGCGGG ATCAAGTTAT 
TTGAGTAGTT TGCTGATTTC TATTATCCAT AGGGCGACAG CTAAATCTTC AACTGGGAAT 
TGGTTACCTC AGGATCTCAA TAAGGGGAGA TTGGATTATT TTTATTACAT TATAACTGCT 
CTAGAAGTCG TTAATTTCGG TTACTTTATA TTGTGTGCCA AGTGGTATAA ATACAAAGGG 
ACTGGTTCTA GCAGCAGTGG TGACCTTCAA CTCGATCAAG TATCCAAACC ATCCGAAAGA 
ACTGTTAACA CTGTCTAG