N2 cDNA ORF clone, Glycine max(soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	N2
Entrez Gene ID	110806310
Full Name	cysteine proteinase inhibitor
Gene Type	protein-coding
Organism	Glycine max(soybean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001354112.1	NP_001341041.1	cysteine proteinase inhibitor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the palaeopolyploid soybean.
Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA
Nature463(7278)178-83(2010 Jan)

Differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate.
Botella MA, Xu Y, Prabha TN, Zhao Y, Narasimhan ML, Wilson KA, Nielsen SS, Bressan RA, Hasegawa PM
Plant physiology112(3)1201-10(1996 Nov)

Two wound-inducible soybean cysteine proteinase inhibitors have greater insect digestive proteinase inhibitory activities than a constitutive homolog.
Zhao Y, Botella MA, Subramanian L, Niu X, Nielsen SS, Bressan RA, Hasegawa PM
Plant physiology111(4)1299-306(1996 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following N2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGl84325	NM_001354112.1 Latest version!	Glycine max cysteine proteinase inhibitor (N2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

N2 ( NM_001354112.1 ) cDNA ORF clone, Glycine max(soybean) -> NP_001341041.1 Glycine max cysteine proteinase inhibitor (N2), mRNA.

Price & Availability↑

CloneID	OGl84325
Clone ID Related Accession (Same CDS sequence)	NM_001354112.1
Accession Version	NM_001354112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-18
Organism	Glycine max(soybean)
Product	cysteine proteinase inhibitor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FG999762.1 and ACUP03008525.1. ##Evidence-Data-START## Transcript exon combination :: EV276285.1, FG988036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00264986, SAMN00264989 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACAG AAACAAAAGT GCAAGAATTA GGTGGCATCA CCGATGTGCA TGGAGCTGCC 
AACAGCGTCG AGATCAACAA TCTCGCTCGC TTTGCTGTAG AGGAACAAAA CAAAAGAGAG 
AATTCAGTTC TGGAGTTTGT GAGGGTGATT AGTGCAAAGC AGCAAGTGGT TGCTGGAGTG 
AATTACTACA TAACATTGGA AGCAAAAGAT GGTTTGATTA AAAATGAGTA TGAAGCGAAG 
GTTTGGGTGA GGGAATGGTT GAACTCCAAA GAGTTGCTAG AATTCAAGCC AGTCAATGTT 
TCTAGCACAC CAATAGGTGG CATTACCGAT GTGACTGCGA ATAGCCTCGA GATCGACATT 
CTCGCTCGTT TTGCTGTTGA TCAACACAAC AAAAAAGAGA ATGCAAATCT GGAGTTTGTG 
GGGGTGATTA GGGCAAAGCA GCAGGTGGTG GAAGGGTTCA TATACTACAT CACTTTGGAA 
GCAAAAGATG GTGAAACCAA AAATGTGTAT GAAACGAAGG TGTGGGTGAG ATCATGGTTG 
AACTCCAAGG AGGTCCTCGA ATTCAAGCCC ATCAGTATTA ATCCTCTTTC GGTGTCGGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341041.1
CDS	14..616
Translation	MATETKVQELGGITDVHGAANSVEINNLARFAVEEQNKRENSVLEFVRVISAKQQVVAGVNYYITLEAKDGLIKNEYEAKVWVREWLNSKELLEFKPVNVSSTPIGGITDVTANSLEIDILARFAVDQHNKKENANLEFVGVIRAKQQVVEGFIYYITLEAKDGETKNVYETKVWVRSWLNSKEVLEFKPISINPLSVSV

Target ORF information:

RefSeq Version	NM_001354112.1
Organism	Glycine max(soybean)
Definition	Glycine max cysteine proteinase inhibitor (N2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354112.1

ORF Insert Sequence:

ATGGCAACAG AAACAAAAGT GCAAGAATTA GGTGGCATCA CCGATGTGCA TGGAGCTGCC 
AACAGCGTCG AGATCAACAA TCTCGCTCGC TTTGCTGTAG AGGAACAAAA CAAAAGAGAG 
AATTCAGTTC TGGAGTTTGT GAGGGTGATT AGTGCAAAGC AGCAAGTGGT TGCTGGAGTG 
AATTACTACA TAACATTGGA AGCAAAAGAT GGTTTGATTA AAAATGAGTA TGAAGCGAAG 
GTTTGGGTGA GGGAATGGTT GAACTCCAAA GAGTTGCTAG AATTCAAGCC AGTCAATGTT 
TCTAGCACAC CAATAGGTGG CATTACCGAT GTGACTGCGA ATAGCCTCGA GATCGACATT 
CTCGCTCGTT TTGCTGTTGA TCAACACAAC AAAAAAGAGA ATGCAAATCT GGAGTTTGTG 
GGGGTGATTA GGGCAAAGCA GCAGGTGGTG GAAGGGTTCA TATACTACAT CACTTTGGAA 
GCAAAAGATG GTGAAACCAA AAATGTGTAT GAAACGAAGG TGTGGGTGAG ATCATGGTTG 
AACTCCAAGG AGGTCCTCGA ATTCAAGCCC ATCAGTATTA ATCCTCTTTC GGTGTCGGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the palaeopolyploid soybean.
Nature463(7278)178-83(2010 Jan)
Schmutz J,Cannon SB,Schlueter J,Ma J,Mitros T,Nelson W,Hyten DL,Song Q,Thelen JJ,Cheng J,Xu D,Hellsten U,May GD,Yu Y,Sakurai T,Umezawa T,Bhattacharyya MK,Sandhu D,Valliyodan B,Lindquist E,Peto M,Grant D,Shu S,Goodstein D,Barry K,Futrell-Griggs M,Abernathy B,Du J,Tian Z,Zhu L,Gill N,Joshi T,Libault M,Sethuraman A,Zhang XC,Shinozaki K,Nguyen HT,Wing RA,Cregan P,Specht J,Grimwood J,Rokhsar D,Stacey G,Shoemaker RC,Jackson SA

Differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate.
Plant physiology112(3)1201-10(1996 Nov)
Botella MA,Xu Y,Prabha TN,Zhao Y,Narasimhan ML,Wilson KA,Nielsen SS,Bressan RA,Hasegawa PM

Two wound-inducible soybean cysteine proteinase inhibitors have greater insect digestive proteinase inhibitory activities than a constitutive homolog.
Plant physiology111(4)1299-306(1996 Aug)
Zhao Y,Botella MA,Subramanian L,Niu X,Nielsen SS,Bressan RA,Hasegawa PM