SCAM-4 cDNA ORF clone, Glycine max(soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCAM-4
Entrez Gene ID	547787
Full Name	calmodulin
Synonyms	cam-4
Gene Type	protein-coding
Organism	Glycine max(soybean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001250973.1	NP_001237902.1	calmodulin
NM_001250973.2	NP_001237902.1	calmodulin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparing the calcium binding abilities of two soybean calmodulins: towards understanding the divergent nature of plant calmodulins.
Gifford JL, Jamshidiha M, Mo J, Ishida H, Vogel HJ
The Plant cell25(11)4512-24(2013 Nov)

Structural studies of soybean calmodulin isoform 4 bound to the calmodulin-binding domain of tobacco mitogen-activated protein kinase phosphatase-1 provide insights into a sequential target binding mode.
Ishida H, Rainaldi M, Vogel HJ
The Journal of biological chemistry284(41)28292-305(2009 Oct)

The solution structures of two soybean calmodulin isoforms provide a structural basis for their selective target activation properties.
Ishida H, Huang H, Yamniuk AP, Takaya Y, Vogel HJ
The Journal of biological chemistry283(21)14619-28(2008 May)

Pathogen-induced binding of the soybean zinc finger homeodomain proteins GmZF-HD1 and GmZF-HD2 to two repeats of ATTA homeodomain binding site in the calmodulin isoform 4 (GmCaM4) promoter.
Park HC, Kim ML, Lee SM, Bahk JD, Yun DJ, Lim CO, Hong JC, Lee SY, Cho MJ, Chung WS
Nucleic acids research35(11)3612-23(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ca2+ binds CaM4 through a semisequential binding pathway favoring the C-terminal EF-hands.
Title: Comparing the calcium binding abilities of two soybean calmodulins: towards understanding the divergent nature of plant calmodulins.

Results provide a high resolution solution structure of the complex of soybean Ca(2+)-CaM4 (SCaM4) and the calmodulin-binding domain region of Nicotiana tabacum MAPK phosphatase-1.
Title: Structural studies of soybean calmodulin isoform 4 bound to the calmodulin-binding domain of tobacco mitogen-activated protein kinase phosphatase-1 provide insights into a sequential target binding mode.

despite the high sequence identity with aCaM, the C-lobe of Ca(2+)-sCaM1 has a more open conformation and consequently a larger hydrophobic target-protein binding pocket than Ca(2+)-aCaM or -sCaM4
Title: The solution structures of two soybean calmodulin isoforms provide a structural basis for their selective target activation properties.

Results suggest that the GmZF-HD1 and -2 proteins function as transcription factors to activate GmCaM4 gene expression in response to pathogen.
Title: Pathogen-induced binding of the soybean zinc finger homeodomain proteins GmZF-HD1 and GmZF-HD2 to two repeats of ATTA homeodomain binding site in the calmodulin isoform 4 (GmCaM4) promoter.

The following SCAM-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCAM-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGl54540	NM_001250973.1 Latest version!	Glycine max calmodulin (SCAM-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OGl84525	NM_001250973.2 Latest version!	Glycine max calmodulin (SCAM-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCAM-4 ( NM_001250973.1 ) cDNA ORF clone, Glycine max(soybean) -> NP_001237902.1 Glycine max calmodulin (SCAM-4), mRNA.

Price & Availability↑

CloneID	OGl54540
Clone ID Related Accession (Same CDS sequence)	NM_001250973.1
Accession Version	NM_001250973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-28
Organism	Glycine max(Soybean)
Product	calmodulin
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT091456.1. ##Evidence-Data-START## Transcript exon combination :: BT091456.1, EV271157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03003437, SAMN03784697 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGATA TCCTGAGTGA AGAACAGATT GTTGATTTTA AAGAGGCCTT TGGCTTGTTT 
GACAAAGATG GGGATGGTTG CATTACTGTG GAAGAACTTG CCACTGTCAT TCGGTCATTG 
GATCAGAACC CCACTGAAGA AGAGCTCCAA GATATGATAA GCGAAGTCGA TGCAGATGGC 
AATGGAACCA TTGAATTTGA CGAGTTCTTG AGCTTGATGG CCAAGAAAGT CAAAGACACT 
GATGCAGAGG AGGAGCTCAA AGAAGCTTTC AAGGTTTTTG ACAAAGATCA AAATGGCTAC 
ATATCAGCTA GTGAGTTGAG ACACGTAATG ATCAATCTAG GGGAAAAACT AACCGATGAA 
GAGGTGGAGC AGATGATTAA AGAAGCAGAT TTGGACGGTG ATGGCCAAGT TAACTATGAG 
GAATTCGTCA AGATGATGAT GACCGTTCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001237902.1
CDS	93..545
Translation	MADILSEEQIVDFKEAFGLFDKDGDGCITVEELATVIRSLDQNPTEEELQDMISEVDADGNGTIEFDEFLSLMAKKVKDTDAEEELKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEVEQMIKEADLDGDGQVNYEEFVKMMMTVR

Target ORF information:

RefSeq Version	NM_001250973.1
Organism	Glycine max(Soybean)
Definition	Glycine max calmodulin (SCAM-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001250973.1

ORF Insert Sequence:

ATGGCAGATA TCCTGAGTGA AGAACAGATT GTTGATTTTA AAGAGGCCTT TGGCTTGTTT 
GACAAAGATG GGGATGGTTG CATTACTGTG GAAGAACTTG CCACTGTCAT TCGGTCATTG 
GATCAGAACC CCACTGAAGA AGAGCTCCAA GATATGATAA GCGAAGTCGA TGCAGATGGC 
AATGGAACCA TTGAATTTGA CGAGTTCTTG AGCTTGATGG CCAAGAAAGT CAAAGACACT 
GATGCAGAGG AGGAGCTCAA AGAAGCTTTC AAGGTTTTTG ACAAAGATCA AAATGGCTAC 
ATATCAGCTA GTGAGTTGAG ACACGTAATG ATCAATCTAG GGGAAAAACT AACCGATGAA 
GAGGTGGAGC AGATGATTAA AGAAGCAGAT TTGGACGGTG ATGGCCAAGT TAACTATGAG 
GAATTCGTCA AGATGATGAT GACCGTTCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCAM-4 ( NM_001250973.2 ) cDNA ORF clone, Glycine max(soybean) -> NP_001237902.1 Glycine max calmodulin (SCAM-4), mRNA.

Price & Availability↑

CloneID	OGl84525
Clone ID Related Accession (Same CDS sequence)	NM_001250973.2
Accession Version	NM_001250973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-01
Organism	Glycine max(soybean)
Product	calmodulin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L01433.1, BI785610.1 and BI424431.1. On Jun 2, 2017 this sequence version replaced NM_001250973.1. ##Evidence-Data-START## Transcript exon combination :: BT091456.1, L01433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03003437, SAMN03784697 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGATA TCCTGAGTGA AGAACAGATT GTTGATTTTA AAGAGGCCTT TGGCTTGTTT 
GACAAAGATG GAGATGGTTG CATTACTGTG GAAGAACTTG CCACTGTCAT TCGGTCATTG 
GATCAGAACC CCACTGAAGA AGAGCTCCAA GATATGATAA GCGAAGTCGA TGCAGATGGC 
AATGGAACCA TTGAATTTGA CGAGTTCTTG AGCTTGATGG CCAAGAAAGT CAAAGACACT 
GATGCAGAGG AGGAGCTCAA AGAAGCTTTC AAGGTTTTTG ACAAAGATCA AAATGGCTAC 
ATATCAGCTA GTGAGTTGAG ACACGTAATG ATCAATCTAG GGGAAAAACT AACCGATGAA 
GAGGTGGAGC AGATGATTAA AGAAGCAGAT TTGGACGGTG ATGGCCAAGT TAACTATGAG 
GAATTCGTCA AGATGATGAT GACCGTTCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001237902.1
CDS	91..543
Translation	MADILSEEQIVDFKEAFGLFDKDGDGCITVEELATVIRSLDQNPTEEELQDMISEVDADGNGTIEFDEFLSLMAKKVKDTDAEEELKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEVEQMIKEADLDGDGQVNYEEFVKMMMTVR

Target ORF information:

RefSeq Version	NM_001250973.2
Organism	Glycine max(soybean)
Definition	Glycine max calmodulin (SCAM-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001250973.2

ORF Insert Sequence:

ATGGCAGATA TCCTGAGTGA AGAACAGATT GTTGATTTTA AAGAGGCCTT TGGCTTGTTT 
GACAAAGATG GAGATGGTTG CATTACTGTG GAAGAACTTG CCACTGTCAT TCGGTCATTG 
GATCAGAACC CCACTGAAGA AGAGCTCCAA GATATGATAA GCGAAGTCGA TGCAGATGGC 
AATGGAACCA TTGAATTTGA CGAGTTCTTG AGCTTGATGG CCAAGAAAGT CAAAGACACT 
GATGCAGAGG AGGAGCTCAA AGAAGCTTTC AAGGTTTTTG ACAAAGATCA AAATGGCTAC 
ATATCAGCTA GTGAGTTGAG ACACGTAATG ATCAATCTAG GGGAAAAACT AACCGATGAA 
GAGGTGGAGC AGATGATTAA AGAAGCAGAT TTGGACGGTG ATGGCCAAGT TAACTATGAG 
GAATTCGTCA AGATGATGAT GACCGTTCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparing the calcium binding abilities of two soybean calmodulins: towards understanding the divergent nature of plant calmodulins.
The Plant cell25(11)4512-24(2013 Nov)
Gifford JL,Jamshidiha M,Mo J,Ishida H,Vogel HJ

Structural studies of soybean calmodulin isoform 4 bound to the calmodulin-binding domain of tobacco mitogen-activated protein kinase phosphatase-1 provide insights into a sequential target binding mode.
The Journal of biological chemistry284(41)28292-305(2009 Oct)
Ishida H,Rainaldi M,Vogel HJ

The solution structures of two soybean calmodulin isoforms provide a structural basis for their selective target activation properties.
The Journal of biological chemistry283(21)14619-28(2008 May)
Ishida H,Huang H,Yamniuk AP,Takaya Y,Vogel HJ

Pathogen-induced binding of the soybean zinc finger homeodomain proteins GmZF-HD1 and GmZF-HD2 to two repeats of ATTA homeodomain binding site in the calmodulin isoform 4 (GmCaM4) promoter.
Nucleic acids research35(11)3612-23(2007)
Park HC,Kim ML,Lee SM,Bahk JD,Yun DJ,Lim CO,Hong JC,Lee SY,Cho MJ,Chung WS