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CXCL8 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CXCL8
Entrez Gene ID 3576
Full Name C-X-C motif chemokine ligand 8
Synonyms GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
General protein information
Preferred Names

C-X-C motif chemokine ligand 8

Names

interleukin-8
T-cell chemotactic factor
alveolar macrophage chemotactic factor I
beta endothelial cell-derived neutrophil activating peptide
beta-thromboglobulin-like protein
chemokine (C-X-C motif) ligand 8
emoctakin
granulocyte chemotactic protein 1
interleukin 8
lung giant cell carcinoma-derived chemotactic protein
lymphocyte derived neutrophil activating peptide
monocyte-derived neutrophil chemotactic factor
monocyte-derived neutrophil-activating peptide
neutrophil-activating peptide 1
small inducible cytokine subfamily B, member 8
tumor necrosis factor-induced gene 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

4

4q13.3

Summary The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008].
Disorder MIM:

146930


mRNA and Protein(s)

mRNA Protein Name
NM_000584.3 NP_000575.1 interleukin-8 precursor



Macaca mulatta (rhesus monkey) IL8 NP_001028137.1
Homo sapiens (human) IL8 NP_000575.1
Pan troglodytes (chimpanzee) IL8 XP_001156432.1
Canis lupus familiaris (dog) IL8 NP_001003200.1
Bos taurus (cattle) IL8 NP_776350.1


Related articles in PubMed

Cot kinase plays a critical role in Helicobacter pylori-induced IL-8 expression.
Jang S, Kim J, Cha JH
Journal of microbiology (Seoul, Korea)55(4)311-317(2017 Apr)

Abnormal peritoneal regulation of chemokine activation-The role of IL-8 in pathogenesis of endometriosis.
Sikora J, Smycz-Kubańska M, Mielczarek-Palacz A, Kondera-Anasz Z
American journal of reproductive immunology (New York, N.Y. : 1989)77(4)(2017 Apr)

CXCL8 induces epithelial-mesenchymal transition in colon cancer cells via the PI3K/Akt/NF-κB signaling pathway.
Shen T, Yang Z, Cheng X, Xiao Y, Yu K, Cai X, Xia C, Li Y
Oncology reports37(4)2095-2100(2017 Apr)

IL-8 -251A/T polymorphism contributes to coronary artery disease susceptibility in a Chinese population.
Zhang RJ, Li XD, Zhang SW, Li XH, Wu L
Genetics and molecular research : GMR16(1)(2017 Feb)

Association between higher expression of interleukin-8 (IL-8) and haplotype -353A/-251A/+678T of IL-8 gene with preeclampsia: A case-control study.
Sun L, Mao D, Cai Y, Tan W, Hao Y, Li L, Liu W
Medicine95(52)e5537(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IL-8 and GRO-alpha were measured by enzyme immunoassay in the first 48 h. After multiple regression analysis only absence of preeclampsia was associated with high IL-8 levels
Title: Chemokines plasma levels in preterm newborns of preeclamptic mothers.

Findings demonstrate an unequivocal role for MACs in angiogenesis, which is linked to paracrine release of cytokines such as IL-8.
Title: Myeloid angiogenic cells act as alternative M2 macrophages and modulate angiogenesis through interleukin-8.

Polymorphisms in IL-8 did not influence the thrombotic risk and the cytokine levels in study participants.
Title: The role of IL-6, IL-8 and MCP-1 and their promoter polymorphisms IL-6 -174GC, IL-8 -251AT and MCP-1 -2518AG in the risk of venous thromboembolism: a case-control study.

Cytokine concentrations in amniotic fluid during the mid-trimester did not differ with parity or fetal gender. IL-6, IL-8, and LBP levels appeared stable with gestational week (GW), whereas GW significantly influenced TNF-alpha concentrations.
Title: Mid-trimester amniotic fluid concentrations of the proinflammatory cytokines IL-6, IL-8, TNF-α, and lipopolysaccharide binding protein in normal pregnancies: a prospective evaluation according to parity, gestational age, and fetal gender.

a novel inflammatory crosstalk between natural killer cells and eosinophils via IL-15/IL-8 axis that can be modulated by vitamin D(3).
Title: Modulation of NK cell autocrine-induced eosinophil chemotaxis by interleukin-15 and vitamin D(3): a possible NK-eosinophil crosstalk via IL-8 in the pathophysiology of allergic rhinitis.

The following CXCL8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CXCL8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20145 NM_000584.3
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Homo sapiens C-X-C motif chemokine ligand 8 (CXCL8), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20145
Clone ID Related Accession (Same CDS sequence) NM_000584.3
Accession Version NM_000584.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product interleukin-8 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG497712.1 and BC013615.1. This sequence is a reference standard in the RefSeqGene project. On Feb 25, 2011 this sequence version replaced NM_000584.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M26383.1, AK131067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
ATGACTTCCA AGCTGGCCGT GGCTCTCTTG GCAGCCTTCC TGATTTCTGC AGCTCTGTGT 
GAAGGTGCAG TTTTGCCAAG GAGTGCTAAA GAACTTAGAT GTCAGTGCAT AAAGACATAC
TCCAAACCTT TCCACCCCAA ATTTATCAAA GAACTGAGAG TGATTGAGAG TGGACCACAC
TGCGCCAACA CAGAAATTAT TGTAAAGCTT TCTGATGGAA GAGAGCTCTG TCTGGACCCC
AAGGAAAACT GGGTGCAGAG GGTTGTGGAG AAGTTTTTGA AGAGGGCTGA GAATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000575.1
CDS154..453
Misc Feature(1)58..60
Misc Feature(2)232..237
Misc Feature(3)232..234
Misc Feature(4)232..234
Misc Feature(5)235..240
Misc Feature(6)235..237
Misc Feature(7)244..435
Misc Feature(8)order(244..252,268..270,283..300,325..333,361..366,373..381,397..399,412..414)
Misc Feature(9)244..252
Misc Feature(10)order(247..249,256..258,298..303,310..318,346..357,361..363,373..378,385..387,406..408,418..420,427..432)
Misc Feature(11)order(247..249,256..258,310..312,316..318,346..348,352..354,361..363,373..378)
Misc Feature(12)order(250..255,259..261)
Misc Feature(13)order(256..276,283..300)
Misc Feature(14)order(262..267,271..273,283..285,295..297,352..354,361..363,373..375,379..381)
Misc Feature(15)order(286..288,292..294,394..396,403..405,409..420,424..426,433..435)
Misc Feature(16)order(292..294,301..303,358..360,364..366,373..378)
Misc Feature(17)order(298..303,313..315,349..351,355..357,385..387,406..408,418..420,427..432)
Misc Feature(18)325..333
Misc Feature(19)325..333
Misc Feature(20)364..372
Exon (1)1..217
Exon (2)218..353
Exon (3)354..437
Exon (4)438..1705
Translation

Target ORF information:

RefSeq Version NM_000584.3
Organism Homo sapiens(Human)
Definition Homo sapiens C-X-C motif chemokine ligand 8 (CXCL8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000584.3

ORF Insert Sequence:

1
61
121
181
241
ATGACTTCCA AGCTGGCCGT GGCTCTCTTG GCAGCCTTCC TGATTTCTGC AGCTCTGTGT 
GAAGGTGCAG TTTTGCCAAG GAGTGCTAAA GAACTTAGAT GTCAGTGCAT AAAGACATAC
TCCAAACCTT TCCACCCCAA ATTTATCAAA GAACTGAGAG TGATTGAGAG TGGACCACAC
TGCGCCAACA CAGAAATTAT TGTAAAGCTT TCTGATGGAA GAGAGCTCTG TCTGGACCCC
AAGGAAAACT GGGTGCAGAG GGTTGTGGAG AAGTTTTTGA AGAGGGCTGA GAATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Cot kinase plays a critical role in Helicobacter pylori-induced IL-8 expression.
Journal of microbiology (Seoul, Korea)55(4)311-317(2017 Apr)
Jang S,Kim J,Cha JH


book

Abnormal peritoneal regulation of chemokine activation-The role of IL-8 in pathogenesis of endometriosis.
American journal of reproductive immunology (New York, N.Y. : 1989)77(4)(2017 Apr)
Sikora J,Smycz-Kubańska M,Mielczarek-Palacz A,Kondera-Anasz Z


book

CXCL8 induces epithelial-mesenchymal transition in colon cancer cells via the PI3K/Akt/NF-κB signaling pathway.
Oncology reports37(4)2095-2100(2017 Apr)
Shen T,Yang Z,Cheng X,Xiao Y,Yu K,Cai X,Xia C,Li Y


book

IL-8 -251A/T polymorphism contributes to coronary artery disease susceptibility in a Chinese population.
Genetics and molecular research : GMR16(1)(2017 Feb)
Zhang RJ,Li XD,Zhang SW,Li XH,Wu L


book

Association between higher expression of interleukin-8 (IL-8) and haplotype -353A/-251A/+678T of IL-8 gene with preeclampsia: A case-control study.
Medicine95(52)e5537(2016 Dec)
Sun L,Mao D,Cai Y,Tan W,Hao Y,Li L,Liu W


 
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