LOC8033840 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8033840
Entrez Gene ID	8033840
Full Name	E3 ubiquitin ligase, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029992997.1	XP_029848857.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_029993004.1	XP_029848864.1	E3 ubiquitin-protein ligase HECW2 isoform X3
XM_002406234.2	XP_002406278.1	E3 ubiquitin ligase, putative
XM_029992999.1	XP_029848859.1	E3 ubiquitin-protein ligase HECW2 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC8033840 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8033840 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa16373	XM_029992997.1 Latest version!	Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa16374	XM_029992999.1 Latest version!	Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa16375	XM_029993004.1 Latest version!	Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8033840 ( XM_029992997.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029848857.1 Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa16373
Clone ID Related Accession (Same CDS sequence)	XM_029992997.1
Accession Version	XM_029992997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021917992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG GCGCGTGCCG TCGCCGCCGG CCGGCCATGA TTGCCGATCG GGGCCCCCTC 
CACGCGGCCG GCAGTGGGCT CCGACTTTCA GCCGCTACCC CGGCCCCCGT GGCGCCCTCG 
CCACACCAGC AGCAGCAGCC GCCGGTGCCC CGCAGGGGCA CGGGGTCGGT GAGCACGGGT 
TCGTCGCCGC CTCCCCCTGC GCCGGCAGAG GCCCCCGGCG TGCCGCCCCG GCGCCTTCCC 
GCACTACAGG GGCGCTCCAG CCTGAGCGTG TCGCGGTCCC AGTACGTGCT GGGACGCACG 
GAGGCCCCGC TGCTGGTCAG CTGGGACATC CGGGAGGAGG TCTCCAAAGA GGACTGGATT 
GGCCTGTACC GCACGGATGA GACGGATCCT CTGCATTTCC TGGAGCACAA GAACAAGCGA 
TGTTTGAGCA ACACTGTCAA GGGGCAGGTC CTTTGGAGCA TAGACTGCAA GGACGCTTTT 
ACCGAAGAGA GGACCAATGT GTGTTTCCGC TACTACCACG GCCCTACGGG TACCCTGAGG 
GCCGTGAGCC CGGTGGTGAC CGTCTGCAGG TCAGCCGACA CCACTGGAAT CGTGAACGGC 
CACGGGTCCG AGGTCCTGGT CGGTTACTCC TCGCAGCAGA TACCGATGGA GATGGTCAAG 
TTCTATTTGA CCAACCTGCA TGCCCGCAAC CTGAAGAAGG GGATGTTCTT CAGCCCGGAC 
CCGTACGTGA AGATGGGCAT CCAGCCGGGC CTGGGGGACG GGGTCCTGCT GCTGCCCCAC 
CACGGCCAGA TCTGCCGCAC GGGGGCTGCC GAGTCCACCG TCCATCCGTC CTGGCCGGGG 
CAGTTTGAGT TTATCGGATA CCCGTCGGAC GTGCTCGAGT TTGAGGTCAA GGACAAGTTT 
GCCCGGAGCC GCCCCATCAT TGGCCGCTTC CTGGGCAGGC TCGTGGTGCG CATCTGGGAC 
CTGCTGGACC GATGCTCCGG GGGCGTACCG ATGGAGCTCA GCTACACTCT GTCCAACAAG 
ACACCACCCG AGCACATGAC GGGCCAACTC TTCTTCACTT TTGTACTGGA ACATGGGCCA 
GTGGACCCGT GGACCCACAT CCGCCAAGTC CACAATCCGA TCTACGTGTC GGACGTCCCG 
CCGGACCCCT GCCCTTCCAG CCCCCCGTCC TCCGAAGTGC CTCCCCCTCC GCCTTCCACC 
CTGCACCAGC CGCCGCTCAC CGCACGAACG ACCTCCCTCC CCAACGGGAG CGGCCACCAC 
GCCCCTCCGA CCTCCTCCTC CTCTTCCTCG TCCTCCTCCA CCTCCTCGGC AGGAGATCCG 
GAGCCGGCCC CACGGCGGGA CGCCGCACGG AGTCGCAGCG TCCGCTCCAA CAGCGAGCAC 
GGCCTCCACT CACCGGGGGC GGCCCCCCAG GCGGCCTCGC GGCGCCTCTC GCAGACGCCG 
CGACCTCCCG CGAACGCATC GTCGTCGTCC TTCGCGAACC TCGAGTACAT GCTGCAGCGG 
GAGGCCGTCC CCGGGACCGG CGCGGACTTG CCGCCGCCGC TGCCTCCAAA GCAGAAGGGT 
AACCCCCGGC GGCATCACCA TCATCATCAT CACCGGCCGT TGGAGAGGTC GCGCGCGTTG 
GATGTGGCGA GGTCGACGAC GGAGGAGGTG ACTTCTTCGT CGTGGAAGCA CGCGAGACAC 
CGGGAGGCGG GCTCACCGGG ACCGGGCACG GGGGGTGAGG CCGTCGACTC GGACGGGAGT 
TCGGCAAGCT TGGCATCTTC AAGTGCAGCG TCCCCGACAG CGACGACCGG CGAGGAAGAG 
GCGAGCCCGC CCCCAACGCT GCCCCGGAGG ACGGCGAGAC CCACCGTGCT CCAGACAACG 
TCGGGCGAAG CGGGCACCGT CGGCAGCGGG AGCGCAAGGG CGGCCCCCCT GGCGACTCCG 
GAGCAGGACT CGTCGCTCCA GGAGGGGGCA CCCTCCTCCC TGGAGAACCT GAGCGGGGTC 
CTGGACGCCC TGGACTCGGA CGAGGAGGTG GGACGGGCCG AGGACGAACG GTCGGCGTGC 
GACTTTGACC GCGCCAACAG CGAGCCGGCC AACCTGGAGT TCGACGCCGG GGGCGTCGCG 
TCCCCTTCGG CCGGAGACGA GGACAACAGC CCCTCTCCCG CGCCAGGGGG CAGCCGGGGG 
GCCTCGCCGC AGCAGTCATC CCTGGGGGAC TCATCCGTGG ACGCTTCGTC GTCCCAGACG 
CTGAGCGCGA GCGAGGAGGA GGAAGAGGAC AACGCGGAGG CGAGGAACTT CGAGTCAGGT 
CGAGAGCAGT CGTCGCAGCG CAGACGGCGT CAGACGCCGC AGCGATGCCA CTCGGACTCG 
GAGTGCTCGT TCCCACGCCC GCGCCGGCAG CACAACCGCC ACAGCCACCA CCAGGCGCAC 
CACCGGAGGC GGCAGCTAGG AGCCGAGGCC AGGCCAGATC CCCCAAGGCT GGACCCCTCG 
TCGAGGGCTG CCTTCGTGTT CCCCGTGGAA CAGTCCGGCT GCCTGGCGTC GTCCGGCAGC 
GTGCCCCTGC TGCTGCCCTA CAGCCAGAGC GACCACGGCA GCCTGTCCCT GGGTCCCCCC 
GAGGAGGCGT GCCCCTTGGG GCCCCGGGGG GCCCTTTCCC GCATGCGCAT GGGCCCCGAG 
GAGTACGGCA GGCTCCTCCC CGTGGGCGAC GGACTCGTCC TGGCGAGTGT GTCAAGCAAC 
AGAACTTCCA CTGTGGAGCC ATCCCCCGCT GCGTCGTCGG TTGCCACCCT GATGAACGTG 
CAGCCGCAGC AGCACAGCAA CACCACCCGG CTGCCTTCCA TACCGGAGCG TACCATCCGC 
TACCAGCGCG TCGAGCTCGA AGAACCGCTA CCCCCCCATT GGGAGGCCCG CATCGACAGT 
CACGGACGCG TCTTCTACAT TGACCACCTG AACCGCACCA CCACGTGGAA CCGGCCCTCT 
CTCTCGCAGC CCGTCAGTGT CGTCGCCTGC AACGAACTCC AGCGGCAACA GTTTGACCGC 
AGGTATCAGA GCATTCGACG CACAATGACG CCACGCATGA CGGCCGCGGC TGCGGAACGA 
GCTTCGGCTG CCGCGGCGGC TGCGGCGGCA GCAGCGGCGG CTTCATCTCC GGTGGCGACA 
TGTGCCGTGG GCATGGGCGC GGCCTCCCCT CCGCCTCCTC CGCCCCCAGC AATCGAGGAG 
GTTGTTCCTG GGAGCTGTTC CTCGGTGGCG CCCTCTGCAC CCCCGGCTCC GGCCCCGGCT 
CCAGTGACAC CGAGTGCCGA CAGCCTAAGG GCGCTGTTGC GGTCGCCCGC AGTTCGCTTT 
GTTACGCGCT CCGACTTCTT CAACGTGCTG CACATGAACG ACGATGCCTT GGCTGCTTAC 
AATAGAAGCT CTTCCTTGAA ACACATGATC GCCAAGATCC GGCGAGATTC CGCCAATTTC 
GAGAGGTACC AACATAACCG GGACCTTGTC AGCCTGCTGA ACAAATTTGC CGACAGGGGA 
AGAGAACTGC CTCGTGGCTG GGAGACAAAG CTAGACCGCA CGGGAAAGCA ATTCTTCATC 
GACCACACGG CGCGGAGCAC GACGTTCATC GACCCCCGGC TACCCATGGA CGTGCCCTAC 
TTGAACCCGA GCCGACTGGT GGTGCCTTTG GCCAGGAGGC GCAGCCGCAG TGCAGGGGAG 
GACGACCGGG CAGAGGCCGC TGCCAGGGCC GGAGGAGCGA CGGGGGGCCC CGTTCCCCCT 
CCCCGGCCCC CGGCGAGCAC GGCGGGGGGC GCGTCTGGGG TGGCGGGCGG GGGCGGCAGC 
GTGCCCACGG CGTACAACGA CAAGGTGGTG GCCTTCCTGC GCCAGCCCAA CATCCTGGAC 
ATCCTGAGGG AGCGCCACCC GGCCATCGCC TCCAACGCCG CCCTAAGGGA CCGCGTCCAC 
TGCATACGCG CCGACGGGAC CGCTGCCCTC GGAAGGCTCT CGCACGACCT CGACCTCACC 
ATCCTGCTCA GCCTGTTTGA GCAGGAGATC ATGTCATACA TTCCGGCCCA GTACACCTCT 
GGGAGCAGTT CTAGTCATCG CTCACCAAGA GATTCTCCGC AGCCTTCGCC GCAGGCCTCC 
CCAGGACTTC AAAGGGCCAA CGTTCGAGCG CCCGCCCCTT ACCGACGGGA CTTTGAGGCC 
AAGCTGCGGA ACTTCTACCG CAAGCTGGAG TCCAAGGGCT ATGGTCAGGG CCCCAGCAAG 
TTAAAACTAA ACATCCGTCG GGATCACCTA CTCGAAGACG CATTCACAAA GATAATGGCT 
GCGTCCAAGA AAGACCTCCA GAAGTCGAGG CTTTACATAG GATTTGCAGG AGAAGAAGGG 
CTGGACTATG GGGGCCCCTC CCGGGAGTTC TTCTTCCTGC TCTCGCGGGA GCTGTTCAAC 
CCGTACTACG GCCTGTTTGA GTACTCAGCC AACGACACGT ACACGGTGCA AGTGAGCCCC 
ATGGCAGCCT TTGTGGACAA CCAGCACGAG TGGTTCCGGT TCAGCGGGCG GGTGCTGGGC 
TTGGCCCTGG TGCACCAGCA CCTGCTCGAC GCCTTCTTCA CCCGGCCCTT CTACAAGGCC 
CTGCTCAGGC TGCCCTGCTC GCTGAGCGAC CTGGAGTACC TGGATGCGGA GTTCCACCAG 
TCGCTCGTCT GGCTGAAGGA GAACGACATC AGCGACATGG GCCTGGAGCT GAGCTTCTCG 
GTGGTGGAGG AGGTGGCCGG CCACGTGCTA GAGCGGGAGC TCAAGCCGGG CGGGCGCTCC 
CTGCCCGTGT CGGAGCGCAA CAAGAAGGAG TACATCGAGC GCATGCTCAA GTGGCGCCTG 
GAGCGGGGCG TTGCCGACCA GACGGAGTGC CTCGTCAGGG GCTTCTACGA GGTCGTCGAC 
TCTCGTCTGG TATCCGTCTT CGACGCCCGA GAGCTGGAGC TGGTGATCGC GGGGACGGCG 
GAGATAGACA CGGCGGACTG GAGGAAAAAC ACTGAGTACC GATCGGGCTA CCACGACGGC 
CACCCCGTCA TCCAGTGGTT CTGGCTGGCC ATTGAGAAAT TTGACAACGA GCGTCGCCTG 
CGGCTGCTCC AGTTTGTCAC GGGCACCTCG AGCATCCCGT ACGAGGGCTT CGCCGCGCTG 
CGCGGCAGCA ACGGGCCGCG CAAGTTCTGC ATCGAGAAGT GGGGGAAGCC CACCAGCCTG 
CCCAGAGCTC ACACCTGCTT CAACCGTCTG GACCTTCCTC CGTACACGTC CTTCGACATG 
CTGTACGAGA AGCTGCTGCT GGCCGTCGAA GAGTCCAGCA CCTTCGGCAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029848857.1
CDS	137..5413
Translation	MAGGACRRRRPAMIADRGPLHAAGSGLRLSAATPAPVAPSPHQQQQPPVPRRGTGSVSTGSSPPPPAPAEAPGVPPRRLPALQGRSSLSVSRSQYVLGRTEAPLLVSWDIREEVSKEDWIGLYRTDETDPLHFLEHKNKRCLSNTVKGQVLWSIDCKDAFTEERTNVCFRYYHGPTGTLRAVSPVVTVCRSADTTGIVNGHGSEVLVGYSSQQIPMEMVKFYLTNLHARNLKKGMFFSPDPYVKMGIQPGLGDGVLLLPHHGQICRTGAAESTVHPSWPGQFEFIGYPSDVLEFEVKDKFARSRPIIGRFLGRLVVRIWDLLDRCSGGVPMELSYTLSNKTPPEHMTGQLFFTFVLEHGPVDPWTHIRQVHNPIYVSDVPPDPCPSSPPSSEVPPPPPSTLHQPPLTARTTSLPNGSGHHAPPTSSSSSSSSSTSSAGDPEPAPRRDAARSRSVRSNSEHGLHSPGAAPQAASRRLSQTPRPPANASSSSFANLEYMLQREAVPGTGADLPPPLPPKQKGNPRRHHHHHHHRPLERSRALDVARSTTEEVTSSSWKHARHREAGSPGPGTGGEAVDSDGSSASLASSSAASPTATTGEEEASPPPTLPRRTARPTVLQTTSGEAGTVGSGSARAAPLATPEQDSSLQEGAPSSLENLSGVLDALDSDEEVGRAEDERSACDFDRANSEPANLEFDAGGVASPSAGDEDNSPSPAPGGSRGASPQQSSLGDSSVDASSSQTLSASEEEEEDNAEARNFESGREQSSQRRRRQTPQRCHSDSECSFPRPRRQHNRHSHHQAHHRRRQLGAEARPDPPRLDPSSRAAFVFPVEQSGCLASSGSVPLLLPYSQSDHGSLSLGPPEEACPLGPRGALSRMRMGPEEYGRLLPVGDGLVLASVSSNRTSTVEPSPAASSVATLMNVQPQQHSNTTRLPSIPERTIRYQRVELEEPLPPHWEARIDSHGRVFYIDHLNRTTTWNRPSLSQPVSVVACNELQRQQFDRRYQSIRRTMTPRMTAAAAERASAAAAAAAAAAAASSPVATCAVGMGAASPPPPPPPAIEEVVPGSCSSVAPSAPPAPAPAPVTPSADSLRALLRSPAVRFVTRSDFFNVLHMNDDALAAYNRSSSLKHMIAKIRRDSANFERYQHNRDLVSLLNKFADRGRELPRGWETKLDRTGKQFFIDHTARSTTFIDPRLPMDVPYLNPSRLVVPLARRRSRSAGEDDRAEAAARAGGATGGPVPPPRPPASTAGGASGVAGGGGSVPTAYNDKVVAFLRQPNILDILRERHPAIASNAALRDRVHCIRADGTAALGRLSHDLDLTILLSLFEQEIMSYIPAQYTSGSSSSHRSPRDSPQPSPQASPGLQRANVRAPAPYRRDFEAKLRNFYRKLESKGYGQGPSKLKLNIRRDHLLEDAFTKIMAASKKDLQKSRLYIGFAGEEGLDYGGPSREFFFLLSRELFNPYYGLFEYSANDTYTVQVSPMAAFVDNQHEWFRFSGRVLGLALVHQHLLDAFFTRPFYKALLRLPCSLSDLEYLDAEFHQSLVWLKENDISDMGLELSFSVVEEVAGHVLERELKPGGRSLPVSERNKKEYIERMLKWRLERGVADQTECLVRGFYEVVDSRLVSVFDARELELVIAGTAEIDTADWRKNTEYRSGYHDGHPVIQWFWLAIEKFDNERRLRLLQFVTGTSSIPYEGFAALRGSNGPRKFCIEKWGKPTSLPRAHTCFNRLDLPPYTSFDMLYEKLLLAVEESSTFGIE

Target ORF information:

RefSeq Version	XM_029992997.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029992997.1

ORF Insert Sequence:

ATGGCAGGGG GCGCGTGCCG TCGCCGCCGG CCGGCCATGA TTGCCGATCG GGGCCCCCTC 
CACGCGGCCG GCAGTGGGCT CCGACTTTCA GCCGCTACCC CGGCCCCCGT GGCGCCCTCG 
CCACACCAGC AGCAGCAGCC GCCGGTGCCC CGCAGGGGCA CGGGGTCGGT GAGCACGGGT 
TCGTCGCCGC CTCCCCCTGC GCCGGCAGAG GCCCCCGGCG TGCCGCCCCG GCGCCTTCCC 
GCACTACAGG GGCGCTCCAG CCTGAGCGTG TCGCGGTCCC AGTACGTGCT GGGACGCACG 
GAGGCCCCGC TGCTGGTCAG CTGGGACATC CGGGAGGAGG TCTCCAAAGA GGACTGGATT 
GGCCTGTACC GCACGGATGA GACGGATCCT CTGCATTTCC TGGAGCACAA GAACAAGCGA 
TGTTTGAGCA ACACTGTCAA GGGGCAGGTC CTTTGGAGCA TAGACTGCAA GGACGCTTTT 
ACCGAAGAGA GGACCAATGT GTGTTTCCGC TACTACCACG GCCCTACGGG TACCCTGAGG 
GCCGTGAGCC CGGTGGTGAC CGTCTGCAGG TCAGCCGACA CCACTGGAAT CGTGAACGGC 
CACGGGTCCG AGGTCCTGGT CGGTTACTCC TCGCAGCAGA TACCGATGGA GATGGTCAAG 
TTCTATTTGA CCAACCTGCA TGCCCGCAAC CTGAAGAAGG GGATGTTCTT CAGCCCGGAC 
CCGTACGTGA AGATGGGCAT CCAGCCGGGC CTGGGGGACG GGGTCCTGCT GCTGCCCCAC 
CACGGCCAGA TCTGCCGCAC GGGGGCTGCC GAGTCCACCG TCCATCCGTC CTGGCCGGGG 
CAGTTTGAGT TTATCGGATA CCCGTCGGAC GTGCTCGAGT TTGAGGTCAA GGACAAGTTT 
GCCCGGAGCC GCCCCATCAT TGGCCGCTTC CTGGGCAGGC TCGTGGTGCG CATCTGGGAC 
CTGCTGGACC GATGCTCCGG GGGCGTACCG ATGGAGCTCA GCTACACTCT GTCCAACAAG 
ACACCACCCG AGCACATGAC GGGCCAACTC TTCTTCACTT TTGTACTGGA ACATGGGCCA 
GTGGACCCGT GGACCCACAT CCGCCAAGTC CACAATCCGA TCTACGTGTC GGACGTCCCG 
CCGGACCCCT GCCCTTCCAG CCCCCCGTCC TCCGAAGTGC CTCCCCCTCC GCCTTCCACC 
CTGCACCAGC CGCCGCTCAC CGCACGAACG ACCTCCCTCC CCAACGGGAG CGGCCACCAC 
GCCCCTCCGA CCTCCTCCTC CTCTTCCTCG TCCTCCTCCA CCTCCTCGGC AGGAGATCCG 
GAGCCGGCCC CACGGCGGGA CGCCGCACGG AGTCGCAGCG TCCGCTCCAA CAGCGAGCAC 
GGCCTCCACT CACCGGGGGC GGCCCCCCAG GCGGCCTCGC GGCGCCTCTC GCAGACGCCG 
CGACCTCCCG CGAACGCATC GTCGTCGTCC TTCGCGAACC TCGAGTACAT GCTGCAGCGG 
GAGGCCGTCC CCGGGACCGG CGCGGACTTG CCGCCGCCGC TGCCTCCAAA GCAGAAGGGT 
AACCCCCGGC GGCATCACCA TCATCATCAT CACCGGCCGT TGGAGAGGTC GCGCGCGTTG 
GATGTGGCGA GGTCGACGAC GGAGGAGGTG ACTTCTTCGT CGTGGAAGCA CGCGAGACAC 
CGGGAGGCGG GCTCACCGGG ACCGGGCACG GGGGGTGAGG CCGTCGACTC GGACGGGAGT 
TCGGCAAGCT TGGCATCTTC AAGTGCAGCG TCCCCGACAG CGACGACCGG CGAGGAAGAG 
GCGAGCCCGC CCCCAACGCT GCCCCGGAGG ACGGCGAGAC CCACCGTGCT CCAGACAACG 
TCGGGCGAAG CGGGCACCGT CGGCAGCGGG AGCGCAAGGG CGGCCCCCCT GGCGACTCCG 
GAGCAGGACT CGTCGCTCCA GGAGGGGGCA CCCTCCTCCC TGGAGAACCT GAGCGGGGTC 
CTGGACGCCC TGGACTCGGA CGAGGAGGTG GGACGGGCCG AGGACGAACG GTCGGCGTGC 
GACTTTGACC GCGCCAACAG CGAGCCGGCC AACCTGGAGT TCGACGCCGG GGGCGTCGCG 
TCCCCTTCGG CCGGAGACGA GGACAACAGC CCCTCTCCCG CGCCAGGGGG CAGCCGGGGG 
GCCTCGCCGC AGCAGTCATC CCTGGGGGAC TCATCCGTGG ACGCTTCGTC GTCCCAGACG 
CTGAGCGCGA GCGAGGAGGA GGAAGAGGAC AACGCGGAGG CGAGGAACTT CGAGTCAGGT 
CGAGAGCAGT CGTCGCAGCG CAGACGGCGT CAGACGCCGC AGCGATGCCA CTCGGACTCG 
GAGTGCTCGT TCCCACGCCC GCGCCGGCAG CACAACCGCC ACAGCCACCA CCAGGCGCAC 
CACCGGAGGC GGCAGCTAGG AGCCGAGGCC AGGCCAGATC CCCCAAGGCT GGACCCCTCG 
TCGAGGGCTG CCTTCGTGTT CCCCGTGGAA CAGTCCGGCT GCCTGGCGTC GTCCGGCAGC 
GTGCCCCTGC TGCTGCCCTA CAGCCAGAGC GACCACGGCA GCCTGTCCCT GGGTCCCCCC 
GAGGAGGCGT GCCCCTTGGG GCCCCGGGGG GCCCTTTCCC GCATGCGCAT GGGCCCCGAG 
GAGTACGGCA GGCTCCTCCC CGTGGGCGAC GGACTCGTCC TGGCGAGTGT GTCAAGCAAC 
AGAACTTCCA CTGTGGAGCC ATCCCCCGCT GCGTCGTCGG TTGCCACCCT GATGAACGTG 
CAGCCGCAGC AGCACAGCAA CACCACCCGG CTGCCTTCCA TACCGGAGCG TACCATCCGC 
TACCAGCGCG TCGAGCTCGA AGAACCGCTA CCCCCCCATT GGGAGGCCCG CATCGACAGT 
CACGGACGCG TCTTCTACAT TGACCACCTG AACCGCACCA CCACGTGGAA CCGGCCCTCT 
CTCTCGCAGC CCGTCAGTGT CGTCGCCTGC AACGAACTCC AGCGGCAACA GTTTGACCGC 
AGGTATCAGA GCATTCGACG CACAATGACG CCACGCATGA CGGCCGCGGC TGCGGAACGA 
GCTTCGGCTG CCGCGGCGGC TGCGGCGGCA GCAGCGGCGG CTTCATCTCC GGTGGCGACA 
TGTGCCGTGG GCATGGGCGC GGCCTCCCCT CCGCCTCCTC CGCCCCCAGC AATCGAGGAG 
GTTGTTCCTG GGAGCTGTTC CTCGGTGGCG CCCTCTGCAC CCCCGGCTCC GGCCCCGGCT 
CCAGTGACAC CGAGTGCCGA CAGCCTAAGG GCGCTGTTGC GGTCGCCCGC AGTTCGCTTT 
GTTACGCGCT CCGACTTCTT CAACGTGCTG CACATGAACG ACGATGCCTT GGCTGCTTAC 
AATAGAAGCT CTTCCTTGAA ACACATGATC GCCAAGATCC GGCGAGATTC CGCCAATTTC 
GAGAGGTACC AACATAACCG GGACCTTGTC AGCCTGCTGA ACAAATTTGC CGACAGGGGA 
AGAGAACTGC CTCGTGGCTG GGAGACAAAG CTAGACCGCA CGGGAAAGCA ATTCTTCATC 
GACCACACGG CGCGGAGCAC GACGTTCATC GACCCCCGGC TACCCATGGA CGTGCCCTAC 
TTGAACCCGA GCCGACTGGT GGTGCCTTTG GCCAGGAGGC GCAGCCGCAG TGCAGGGGAG 
GACGACCGGG CAGAGGCCGC TGCCAGGGCC GGAGGAGCGA CGGGGGGCCC CGTTCCCCCT 
CCCCGGCCCC CGGCGAGCAC GGCGGGGGGC GCGTCTGGGG TGGCGGGCGG GGGCGGCAGC 
GTGCCCACGG CGTACAACGA CAAGGTGGTG GCCTTCCTGC GCCAGCCCAA CATCCTGGAC 
ATCCTGAGGG AGCGCCACCC GGCCATCGCC TCCAACGCCG CCCTAAGGGA CCGCGTCCAC 
TGCATACGCG CCGACGGGAC CGCTGCCCTC GGAAGGCTCT CGCACGACCT CGACCTCACC 
ATCCTGCTCA GCCTGTTTGA GCAGGAGATC ATGTCATACA TTCCGGCCCA GTACACCTCT 
GGGAGCAGTT CTAGTCATCG CTCACCAAGA GATTCTCCGC AGCCTTCGCC GCAGGCCTCC 
CCAGGACTTC AAAGGGCCAA CGTTCGAGCG CCCGCCCCTT ACCGACGGGA CTTTGAGGCC 
AAGCTGCGGA ACTTCTACCG CAAGCTGGAG TCCAAGGGCT ATGGTCAGGG CCCCAGCAAG 
TTAAAACTAA ACATCCGTCG GGATCACCTA CTCGAAGACG CATTCACAAA GATAATGGCT 
GCGTCCAAGA AAGACCTCCA GAAGTCGAGG CTTTACATAG GATTTGCAGG AGAAGAAGGG 
CTGGACTATG GGGGCCCCTC CCGGGAGTTC TTCTTCCTGC TCTCGCGGGA GCTGTTCAAC 
CCGTACTACG GCCTGTTTGA GTACTCAGCC AACGACACGT ACACGGTGCA AGTGAGCCCC 
ATGGCAGCCT TTGTGGACAA CCAGCACGAG TGGTTCCGGT TCAGCGGGCG GGTGCTGGGC 
TTGGCCCTGG TGCACCAGCA CCTGCTCGAC GCCTTCTTCA CCCGGCCCTT CTACAAGGCC 
CTGCTCAGGC TGCCCTGCTC GCTGAGCGAC CTGGAGTACC TGGATGCGGA GTTCCACCAG 
TCGCTCGTCT GGCTGAAGGA GAACGACATC AGCGACATGG GCCTGGAGCT GAGCTTCTCG 
GTGGTGGAGG AGGTGGCCGG CCACGTGCTA GAGCGGGAGC TCAAGCCGGG CGGGCGCTCC 
CTGCCCGTGT CGGAGCGCAA CAAGAAGGAG TACATCGAGC GCATGCTCAA GTGGCGCCTG 
GAGCGGGGCG TTGCCGACCA GACGGAGTGC CTCGTCAGGG GCTTCTACGA GGTCGTCGAC 
TCTCGTCTGG TATCCGTCTT CGACGCCCGA GAGCTGGAGC TGGTGATCGC GGGGACGGCG 
GAGATAGACA CGGCGGACTG GAGGAAAAAC ACTGAGTACC GATCGGGCTA CCACGACGGC 
CACCCCGTCA TCCAGTGGTT CTGGCTGGCC ATTGAGAAAT TTGACAACGA GCGTCGCCTG 
CGGCTGCTCC AGTTTGTCAC GGGCACCTCG AGCATCCCGT ACGAGGGCTT CGCCGCGCTG 
CGCGGCAGCA ACGGGCCGCG CAAGTTCTGC ATCGAGAAGT GGGGGAAGCC CACCAGCCTG 
CCCAGAGCTC ACACCTGCTT CAACCGTCTG GACCTTCCTC CGTACACGTC CTTCGACATG 
CTGTACGAGA AGCTGCTGCT GGCCGTCGAA GAGTCCAGCA CCTTCGGCAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8033840 ( XM_029992999.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029848859.1 Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIa16374
Clone ID Related Accession (Same CDS sequence)	XM_029992999.1
Accession Version	XM_029992999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021917992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG GCGCGTGCCG TCGCCGCCGG CCGGCCATGA TTGCCGATCG GGGCCCCCTC 
CACGCGGCCG GCAGTGGGCT CCGACTTTCA GCCGCTACCC CGGCCCCCGT GGCGCCCTCG 
CCACACCAGC AGCAGCAGCC GCCGGTGCCC CGCAGGGGCA CGGGGTCGGT GAGCACGGGT 
TCGTCGCCGC CTCCCCCTGC GCCGGCAGAG GCCCCCGGCG TGCCGCCCCG GCGCCTTCCC 
GCACTACAGG GGCGCTCCAG CCTGAGCGTG TCGCGGTCCC AGTACGTGCT GGGACGCACG 
GAGGCCCCGC TGCTGGTCAG CTGGGACATC CGGGAGGAGG TCTCCAAAGA GGACTGGATT 
GGCCTGTACC GCACGGATGA GACGGATCCT CTGCATTTCC TGGAGCACAA GAACAAGCGA 
TGTTTGAGCA ACACTGTCAA GGGGCAGGTC CTTTGGAGCA TAGACTGCAA GGACGCTTTT 
ACCGAAGAGA GGACCAATGT GTGTTTCCGC TACTACCACG GCCCTACGGG TACCCTGAGG 
GCCGTGAGCC CGGTGGTGAC CGTCTGCAGG TCAGCCGACA CCACTGGAAT CAACGGCCAC 
GGGTCCGAGG TCCTGGTCGG TTACTCCTCG CAGCAGATAC CGATGGAGAT GGTCAAGTTC 
TATTTGACCA ACCTGCATGC CCGCAACCTG AAGAAGGGGA TGTTCTTCAG CCCGGACCCG 
TACGTGAAGA TGGGCATCCA GCCGGGCCTG GGGGACGGGG TCCTGCTGCT GCCCCACCAC 
GGCCAGATCT GCCGCACGGG GGCTGCCGAG TCCACCGTCC ATCCGTCCTG GCCGGGGCAG 
TTTGAGTTTA TCGGATACCC GTCGGACGTG CTCGAGTTTG AGGTCAAGGA CAAGTTTGCC 
CGGAGCCGCC CCATCATTGG CCGCTTCCTG GGCAGGCTCG TGGTGCGCAT CTGGGACCTG 
CTGGACCGAT GCTCCGGGGG CGTACCGATG GAGCTCAGCT ACACTCTGTC CAACAAGACA 
CCACCCGAGC ACATGACGGG CCAACTCTTC TTCACTTTTG TACTGGAACA TGGGCCAGTG 
GACCCGTGGA CCCACATCCG CCAAGTCCAC AATCCGATCT ACGTGTCGGA CGTCCCGCCG 
GACCCCTGCC CTTCCAGCCC CCCGTCCTCC GAAGTGCCTC CCCCTCCGCC TTCCACCCTG 
CACCAGCCGC CGCTCACCGC ACGAACGACC TCCCTCCCCA ACGGGAGCGG CCACCACGCC 
CCTCCGACCT CCTCCTCCTC TTCCTCGTCC TCCTCCACCT CCTCGGCAGG AGATCCGGAG 
CCGGCCCCAC GGCGGGACGC CGCACGGAGT CGCAGCGTCC GCTCCAACAG CGAGCACGGC 
CTCCACTCAC CGGGGGCGGC CCCCCAGGCG GCCTCGCGGC GCCTCTCGCA GACGCCGCGA 
CCTCCCGCGA ACGCATCGTC GTCGTCCTTC GCGAACCTCG AGTACATGCT GCAGCGGGAG 
GCCGTCCCCG GGACCGGCGC GGACTTGCCG CCGCCGCTGC CTCCAAAGCA GAAGGGTAAC 
CCCCGGCGGC ATCACCATCA TCATCATCAC CGGCCGTTGG AGAGGTCGCG CGCGTTGGAT 
GTGGCGAGGT CGACGACGGA GGAGGTGACT TCTTCGTCGT GGAAGCACGC GAGACACCGG 
GAGGCGGGCT CACCGGGACC GGGCACGGGG GGTGAGGCCG TCGACTCGGA CGGGAGTTCG 
GCAAGCTTGG CATCTTCAAG TGCAGCGTCC CCGACAGCGA CGACCGGCGA GGAAGAGGCG 
AGCCCGCCCC CAACGCTGCC CCGGAGGACG GCGAGACCCA CCGTGCTCCA GACAACGTCG 
GGCGAAGCGG GCACCGTCGG CAGCGGGAGC GCAAGGGCGG CCCCCCTGGC GACTCCGGAG 
CAGGACTCGT CGCTCCAGGA GGGGGCACCC TCCTCCCTGG AGAACCTGAG CGGGGTCCTG 
GACGCCCTGG ACTCGGACGA GGAGGTGGGA CGGGCCGAGG ACGAACGGTC GGCGTGCGAC 
TTTGACCGCG CCAACAGCGA GCCGGCCAAC CTGGAGTTCG ACGCCGGGGG CGTCGCGTCC 
CCTTCGGCCG GAGACGAGGA CAACAGCCCC TCTCCCGCGC CAGGGGGCAG CCGGGGGGCC 
TCGCCGCAGC AGTCATCCCT GGGGGACTCA TCCGTGGACG CTTCGTCGTC CCAGACGCTG 
AGCGCGAGCG AGGAGGAGGA AGAGGACAAC GCGGAGGCGA GGAACTTCGA GTCAGGTCGA 
GAGCAGTCGT CGCAGCGCAG ACGGCGTCAG ACGCCGCAGC GATGCCACTC GGACTCGGAG 
TGCTCGTTCC CACGCCCGCG CCGGCAGCAC AACCGCCACA GCCACCACCA GGCGCACCAC 
CGGAGGCGGC AGCTAGGAGC CGAGGCCAGG CCAGATCCCC CAAGGCTGGA CCCCTCGTCG 
AGGGCTGCCT TCGTGTTCCC CGTGGAACAG TCCGGCTGCC TGGCGTCGTC CGGCAGCGTG 
CCCCTGCTGC TGCCCTACAG CCAGAGCGAC CACGGCAGCC TGTCCCTGGG TCCCCCCGAG 
GAGGCGTGCC CCTTGGGGCC CCGGGGGGCC CTTTCCCGCA TGCGCATGGG CCCCGAGGAG 
TACGGCAGGC TCCTCCCCGT GGGCGACGGA CTCGTCCTGG CGAGTGTGTC AAGCAACAGA 
ACTTCCACTG TGGAGCCATC CCCCGCTGCG TCGTCGGTTG CCACCCTGAT GAACGTGCAG 
CCGCAGCAGC ACAGCAACAC CACCCGGCTG CCTTCCATAC CGGAGCGTAC CATCCGCTAC 
CAGCGCGTCG AGCTCGAAGA ACCGCTACCC CCCCATTGGG AGGCCCGCAT CGACAGTCAC 
GGACGCGTCT TCTACATTGA CCACCTGAAC CGCACCACCA CGTGGAACCG GCCCTCTCTC 
TCGCAGCCCG TCAGTGTCGT CGCCTGCAAC GAACTCCAGC GGCAACAGTT TGACCGCAGG 
TATCAGAGCA TTCGACGCAC AATGACGCCA CGCATGACGG CCGCGGCTGC GGAACGAGCT 
TCGGCTGCCG CGGCGGCTGC GGCGGCAGCA GCGGCGGCTT CATCTCCGGT GGCGACATGT 
GCCGTGGGCA TGGGCGCGGC CTCCCCTCCG CCTCCTCCGC CCCCAGCAAT CGAGGAGGTT 
GTTCCTGGGA GCTGTTCCTC GGTGGCGCCC TCTGCACCCC CGGCTCCGGC CCCGGCTCCA 
GTGACACCGA GTGCCGACAG CCTAAGGGCG CTGTTGCGGT CGCCCGCAGT TCGCTTTGTT 
ACGCGCTCCG ACTTCTTCAA CGTGCTGCAC ATGAACGACG ATGCCTTGGC TGCTTACAAT 
AGAAGCTCTT CCTTGAAACA CATGATCGCC AAGATCCGGC GAGATTCCGC CAATTTCGAG 
AGGTACCAAC ATAACCGGGA CCTTGTCAGC CTGCTGAACA AATTTGCCGA CAGGGGAAGA 
GAACTGCCTC GTGGCTGGGA GACAAAGCTA GACCGCACGG GAAAGCAATT CTTCATCGAC 
CACACGGCGC GGAGCACGAC GTTCATCGAC CCCCGGCTAC CCATGGACGT GCCCTACTTG 
AACCCGAGCC GACTGGTGGT GCCTTTGGCC AGGAGGCGCA GCCGCAGTGC AGGGGAGGAC 
GACCGGGCAG AGGCCGCTGC CAGGGCCGGA GGAGCGACGG GGGGCCCCGT TCCCCCTCCC 
CGGCCCCCGG CGAGCACGGC GGGGGGCGCG TCTGGGGTGG CGGGCGGGGG CGGCAGCGTG 
CCCACGGCGT ACAACGACAA GGTGGTGGCC TTCCTGCGCC AGCCCAACAT CCTGGACATC 
CTGAGGGAGC GCCACCCGGC CATCGCCTCC AACGCCGCCC TAAGGGACCG CGTCCACTGC 
ATACGCGCCG ACGGGACCGC TGCCCTCGGA AGGCTCTCGC ACGACCTCGA CCTCACCATC 
CTGCTCAGCC TGTTTGAGCA GGAGATCATG TCATACATTC CGGCCCAGTA CACCTCTGGG 
AGCAGTTCTA GTCATCGCTC ACCAAGAGAT TCTCCGCAGC CTTCGCCGCA GGCCTCCCCA 
GGACTTCAAA GGGCCAACGT TCGAGCGCCC GCCCCTTACC GACGGGACTT TGAGGCCAAG 
CTGCGGAACT TCTACCGCAA GCTGGAGTCC AAGGGCTATG GTCAGGGCCC CAGCAAGTTA 
AAACTAAACA TCCGTCGGGA TCACCTACTC GAAGACGCAT TCACAAAGAT AATGGCTGCG 
TCCAAGAAAG ACCTCCAGAA GTCGAGGCTT TACATAGGAT TTGCAGGAGA AGAAGGGCTG 
GACTATGGGG GCCCCTCCCG GGAGTTCTTC TTCCTGCTCT CGCGGGAGCT GTTCAACCCG 
TACTACGGCC TGTTTGAGTA CTCAGCCAAC GACACGTACA CGGTGCAAGT GAGCCCCATG 
GCAGCCTTTG TGGACAACCA GCACGAGTGG TTCCGGTTCA GCGGGCGGGT GCTGGGCTTG 
GCCCTGGTGC ACCAGCACCT GCTCGACGCC TTCTTCACCC GGCCCTTCTA CAAGGCCCTG 
CTCAGGCTGC CCTGCTCGCT GAGCGACCTG GAGTACCTGG ATGCGGAGTT CCACCAGTCG 
CTCGTCTGGC TGAAGGAGAA CGACATCAGC GACATGGGCC TGGAGCTGAG CTTCTCGGTG 
GTGGAGGAGG TGGCCGGCCA CGTGCTAGAG CGGGAGCTCA AGCCGGGCGG GCGCTCCCTG 
CCCGTGTCGG AGCGCAACAA GAAGGAGTAC ATCGAGCGCA TGCTCAAGTG GCGCCTGGAG 
CGGGGCGTTG CCGACCAGAC GGAGTGCCTC GTCAGGGGCT TCTACGAGGT CGTCGACTCT 
CGTCTGGTAT CCGTCTTCGA CGCCCGAGAG CTGGAGCTGG TGATCGCGGG GACGGCGGAG 
ATAGACACGG CGGACTGGAG GAAAAACACT GAGTACCGAT CGGGCTACCA CGACGGCCAC 
CCCGTCATCC AGTGGTTCTG GCTGGCCATT GAGAAATTTG ACAACGAGCG TCGCCTGCGG 
CTGCTCCAGT TTGTCACGGG CACCTCGAGC ATCCCGTACG AGGGCTTCGC CGCGCTGCGC 
GGCAGCAACG GGCCGCGCAA GTTCTGCATC GAGAAGTGGG GGAAGCCCAC CAGCCTGCCC 
AGAGCTCACA CCTGCTTCAA CCGTCTGGAC CTTCCTCCGT ACACGTCCTT CGACATGCTG 
TACGAGAAGC TGCTGCTGGC CGTCGAAGAG TCCAGCACCT TCGGCATCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029848859.1
CDS	137..5410
Translation	MAGGACRRRRPAMIADRGPLHAAGSGLRLSAATPAPVAPSPHQQQQPPVPRRGTGSVSTGSSPPPPAPAEAPGVPPRRLPALQGRSSLSVSRSQYVLGRTEAPLLVSWDIREEVSKEDWIGLYRTDETDPLHFLEHKNKRCLSNTVKGQVLWSIDCKDAFTEERTNVCFRYYHGPTGTLRAVSPVVTVCRSADTTGINGHGSEVLVGYSSQQIPMEMVKFYLTNLHARNLKKGMFFSPDPYVKMGIQPGLGDGVLLLPHHGQICRTGAAESTVHPSWPGQFEFIGYPSDVLEFEVKDKFARSRPIIGRFLGRLVVRIWDLLDRCSGGVPMELSYTLSNKTPPEHMTGQLFFTFVLEHGPVDPWTHIRQVHNPIYVSDVPPDPCPSSPPSSEVPPPPPSTLHQPPLTARTTSLPNGSGHHAPPTSSSSSSSSSTSSAGDPEPAPRRDAARSRSVRSNSEHGLHSPGAAPQAASRRLSQTPRPPANASSSSFANLEYMLQREAVPGTGADLPPPLPPKQKGNPRRHHHHHHHRPLERSRALDVARSTTEEVTSSSWKHARHREAGSPGPGTGGEAVDSDGSSASLASSSAASPTATTGEEEASPPPTLPRRTARPTVLQTTSGEAGTVGSGSARAAPLATPEQDSSLQEGAPSSLENLSGVLDALDSDEEVGRAEDERSACDFDRANSEPANLEFDAGGVASPSAGDEDNSPSPAPGGSRGASPQQSSLGDSSVDASSSQTLSASEEEEEDNAEARNFESGREQSSQRRRRQTPQRCHSDSECSFPRPRRQHNRHSHHQAHHRRRQLGAEARPDPPRLDPSSRAAFVFPVEQSGCLASSGSVPLLLPYSQSDHGSLSLGPPEEACPLGPRGALSRMRMGPEEYGRLLPVGDGLVLASVSSNRTSTVEPSPAASSVATLMNVQPQQHSNTTRLPSIPERTIRYQRVELEEPLPPHWEARIDSHGRVFYIDHLNRTTTWNRPSLSQPVSVVACNELQRQQFDRRYQSIRRTMTPRMTAAAAERASAAAAAAAAAAAASSPVATCAVGMGAASPPPPPPPAIEEVVPGSCSSVAPSAPPAPAPAPVTPSADSLRALLRSPAVRFVTRSDFFNVLHMNDDALAAYNRSSSLKHMIAKIRRDSANFERYQHNRDLVSLLNKFADRGRELPRGWETKLDRTGKQFFIDHTARSTTFIDPRLPMDVPYLNPSRLVVPLARRRSRSAGEDDRAEAAARAGGATGGPVPPPRPPASTAGGASGVAGGGGSVPTAYNDKVVAFLRQPNILDILRERHPAIASNAALRDRVHCIRADGTAALGRLSHDLDLTILLSLFEQEIMSYIPAQYTSGSSSSHRSPRDSPQPSPQASPGLQRANVRAPAPYRRDFEAKLRNFYRKLESKGYGQGPSKLKLNIRRDHLLEDAFTKIMAASKKDLQKSRLYIGFAGEEGLDYGGPSREFFFLLSRELFNPYYGLFEYSANDTYTVQVSPMAAFVDNQHEWFRFSGRVLGLALVHQHLLDAFFTRPFYKALLRLPCSLSDLEYLDAEFHQSLVWLKENDISDMGLELSFSVVEEVAGHVLERELKPGGRSLPVSERNKKEYIERMLKWRLERGVADQTECLVRGFYEVVDSRLVSVFDARELELVIAGTAEIDTADWRKNTEYRSGYHDGHPVIQWFWLAIEKFDNERRLRLLQFVTGTSSIPYEGFAALRGSNGPRKFCIEKWGKPTSLPRAHTCFNRLDLPPYTSFDMLYEKLLLAVEESSTFGIE

Target ORF information:

RefSeq Version	XM_029992999.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029992999.1

ORF Insert Sequence:

ATGGCAGGGG GCGCGTGCCG TCGCCGCCGG CCGGCCATGA TTGCCGATCG GGGCCCCCTC 
CACGCGGCCG GCAGTGGGCT CCGACTTTCA GCCGCTACCC CGGCCCCCGT GGCGCCCTCG 
CCACACCAGC AGCAGCAGCC GCCGGTGCCC CGCAGGGGCA CGGGGTCGGT GAGCACGGGT 
TCGTCGCCGC CTCCCCCTGC GCCGGCAGAG GCCCCCGGCG TGCCGCCCCG GCGCCTTCCC 
GCACTACAGG GGCGCTCCAG CCTGAGCGTG TCGCGGTCCC AGTACGTGCT GGGACGCACG 
GAGGCCCCGC TGCTGGTCAG CTGGGACATC CGGGAGGAGG TCTCCAAAGA GGACTGGATT 
GGCCTGTACC GCACGGATGA GACGGATCCT CTGCATTTCC TGGAGCACAA GAACAAGCGA 
TGTTTGAGCA ACACTGTCAA GGGGCAGGTC CTTTGGAGCA TAGACTGCAA GGACGCTTTT 
ACCGAAGAGA GGACCAATGT GTGTTTCCGC TACTACCACG GCCCTACGGG TACCCTGAGG 
GCCGTGAGCC CGGTGGTGAC CGTCTGCAGG TCAGCCGACA CCACTGGAAT CAACGGCCAC 
GGGTCCGAGG TCCTGGTCGG TTACTCCTCG CAGCAGATAC CGATGGAGAT GGTCAAGTTC 
TATTTGACCA ACCTGCATGC CCGCAACCTG AAGAAGGGGA TGTTCTTCAG CCCGGACCCG 
TACGTGAAGA TGGGCATCCA GCCGGGCCTG GGGGACGGGG TCCTGCTGCT GCCCCACCAC 
GGCCAGATCT GCCGCACGGG GGCTGCCGAG TCCACCGTCC ATCCGTCCTG GCCGGGGCAG 
TTTGAGTTTA TCGGATACCC GTCGGACGTG CTCGAGTTTG AGGTCAAGGA CAAGTTTGCC 
CGGAGCCGCC CCATCATTGG CCGCTTCCTG GGCAGGCTCG TGGTGCGCAT CTGGGACCTG 
CTGGACCGAT GCTCCGGGGG CGTACCGATG GAGCTCAGCT ACACTCTGTC CAACAAGACA 
CCACCCGAGC ACATGACGGG CCAACTCTTC TTCACTTTTG TACTGGAACA TGGGCCAGTG 
GACCCGTGGA CCCACATCCG CCAAGTCCAC AATCCGATCT ACGTGTCGGA CGTCCCGCCG 
GACCCCTGCC CTTCCAGCCC CCCGTCCTCC GAAGTGCCTC CCCCTCCGCC TTCCACCCTG 
CACCAGCCGC CGCTCACCGC ACGAACGACC TCCCTCCCCA ACGGGAGCGG CCACCACGCC 
CCTCCGACCT CCTCCTCCTC TTCCTCGTCC TCCTCCACCT CCTCGGCAGG AGATCCGGAG 
CCGGCCCCAC GGCGGGACGC CGCACGGAGT CGCAGCGTCC GCTCCAACAG CGAGCACGGC 
CTCCACTCAC CGGGGGCGGC CCCCCAGGCG GCCTCGCGGC GCCTCTCGCA GACGCCGCGA 
CCTCCCGCGA ACGCATCGTC GTCGTCCTTC GCGAACCTCG AGTACATGCT GCAGCGGGAG 
GCCGTCCCCG GGACCGGCGC GGACTTGCCG CCGCCGCTGC CTCCAAAGCA GAAGGGTAAC 
CCCCGGCGGC ATCACCATCA TCATCATCAC CGGCCGTTGG AGAGGTCGCG CGCGTTGGAT 
GTGGCGAGGT CGACGACGGA GGAGGTGACT TCTTCGTCGT GGAAGCACGC GAGACACCGG 
GAGGCGGGCT CACCGGGACC GGGCACGGGG GGTGAGGCCG TCGACTCGGA CGGGAGTTCG 
GCAAGCTTGG CATCTTCAAG TGCAGCGTCC CCGACAGCGA CGACCGGCGA GGAAGAGGCG 
AGCCCGCCCC CAACGCTGCC CCGGAGGACG GCGAGACCCA CCGTGCTCCA GACAACGTCG 
GGCGAAGCGG GCACCGTCGG CAGCGGGAGC GCAAGGGCGG CCCCCCTGGC GACTCCGGAG 
CAGGACTCGT CGCTCCAGGA GGGGGCACCC TCCTCCCTGG AGAACCTGAG CGGGGTCCTG 
GACGCCCTGG ACTCGGACGA GGAGGTGGGA CGGGCCGAGG ACGAACGGTC GGCGTGCGAC 
TTTGACCGCG CCAACAGCGA GCCGGCCAAC CTGGAGTTCG ACGCCGGGGG CGTCGCGTCC 
CCTTCGGCCG GAGACGAGGA CAACAGCCCC TCTCCCGCGC CAGGGGGCAG CCGGGGGGCC 
TCGCCGCAGC AGTCATCCCT GGGGGACTCA TCCGTGGACG CTTCGTCGTC CCAGACGCTG 
AGCGCGAGCG AGGAGGAGGA AGAGGACAAC GCGGAGGCGA GGAACTTCGA GTCAGGTCGA 
GAGCAGTCGT CGCAGCGCAG ACGGCGTCAG ACGCCGCAGC GATGCCACTC GGACTCGGAG 
TGCTCGTTCC CACGCCCGCG CCGGCAGCAC AACCGCCACA GCCACCACCA GGCGCACCAC 
CGGAGGCGGC AGCTAGGAGC CGAGGCCAGG CCAGATCCCC CAAGGCTGGA CCCCTCGTCG 
AGGGCTGCCT TCGTGTTCCC CGTGGAACAG TCCGGCTGCC TGGCGTCGTC CGGCAGCGTG 
CCCCTGCTGC TGCCCTACAG CCAGAGCGAC CACGGCAGCC TGTCCCTGGG TCCCCCCGAG 
GAGGCGTGCC CCTTGGGGCC CCGGGGGGCC CTTTCCCGCA TGCGCATGGG CCCCGAGGAG 
TACGGCAGGC TCCTCCCCGT GGGCGACGGA CTCGTCCTGG CGAGTGTGTC AAGCAACAGA 
ACTTCCACTG TGGAGCCATC CCCCGCTGCG TCGTCGGTTG CCACCCTGAT GAACGTGCAG 
CCGCAGCAGC ACAGCAACAC CACCCGGCTG CCTTCCATAC CGGAGCGTAC CATCCGCTAC 
CAGCGCGTCG AGCTCGAAGA ACCGCTACCC CCCCATTGGG AGGCCCGCAT CGACAGTCAC 
GGACGCGTCT TCTACATTGA CCACCTGAAC CGCACCACCA CGTGGAACCG GCCCTCTCTC 
TCGCAGCCCG TCAGTGTCGT CGCCTGCAAC GAACTCCAGC GGCAACAGTT TGACCGCAGG 
TATCAGAGCA TTCGACGCAC AATGACGCCA CGCATGACGG CCGCGGCTGC GGAACGAGCT 
TCGGCTGCCG CGGCGGCTGC GGCGGCAGCA GCGGCGGCTT CATCTCCGGT GGCGACATGT 
GCCGTGGGCA TGGGCGCGGC CTCCCCTCCG CCTCCTCCGC CCCCAGCAAT CGAGGAGGTT 
GTTCCTGGGA GCTGTTCCTC GGTGGCGCCC TCTGCACCCC CGGCTCCGGC CCCGGCTCCA 
GTGACACCGA GTGCCGACAG CCTAAGGGCG CTGTTGCGGT CGCCCGCAGT TCGCTTTGTT 
ACGCGCTCCG ACTTCTTCAA CGTGCTGCAC ATGAACGACG ATGCCTTGGC TGCTTACAAT 
AGAAGCTCTT CCTTGAAACA CATGATCGCC AAGATCCGGC GAGATTCCGC CAATTTCGAG 
AGGTACCAAC ATAACCGGGA CCTTGTCAGC CTGCTGAACA AATTTGCCGA CAGGGGAAGA 
GAACTGCCTC GTGGCTGGGA GACAAAGCTA GACCGCACGG GAAAGCAATT CTTCATCGAC 
CACACGGCGC GGAGCACGAC GTTCATCGAC CCCCGGCTAC CCATGGACGT GCCCTACTTG 
AACCCGAGCC GACTGGTGGT GCCTTTGGCC AGGAGGCGCA GCCGCAGTGC AGGGGAGGAC 
GACCGGGCAG AGGCCGCTGC CAGGGCCGGA GGAGCGACGG GGGGCCCCGT TCCCCCTCCC 
CGGCCCCCGG CGAGCACGGC GGGGGGCGCG TCTGGGGTGG CGGGCGGGGG CGGCAGCGTG 
CCCACGGCGT ACAACGACAA GGTGGTGGCC TTCCTGCGCC AGCCCAACAT CCTGGACATC 
CTGAGGGAGC GCCACCCGGC CATCGCCTCC AACGCCGCCC TAAGGGACCG CGTCCACTGC 
ATACGCGCCG ACGGGACCGC TGCCCTCGGA AGGCTCTCGC ACGACCTCGA CCTCACCATC 
CTGCTCAGCC TGTTTGAGCA GGAGATCATG TCATACATTC CGGCCCAGTA CACCTCTGGG 
AGCAGTTCTA GTCATCGCTC ACCAAGAGAT TCTCCGCAGC CTTCGCCGCA GGCCTCCCCA 
GGACTTCAAA GGGCCAACGT TCGAGCGCCC GCCCCTTACC GACGGGACTT TGAGGCCAAG 
CTGCGGAACT TCTACCGCAA GCTGGAGTCC AAGGGCTATG GTCAGGGCCC CAGCAAGTTA 
AAACTAAACA TCCGTCGGGA TCACCTACTC GAAGACGCAT TCACAAAGAT AATGGCTGCG 
TCCAAGAAAG ACCTCCAGAA GTCGAGGCTT TACATAGGAT TTGCAGGAGA AGAAGGGCTG 
GACTATGGGG GCCCCTCCCG GGAGTTCTTC TTCCTGCTCT CGCGGGAGCT GTTCAACCCG 
TACTACGGCC TGTTTGAGTA CTCAGCCAAC GACACGTACA CGGTGCAAGT GAGCCCCATG 
GCAGCCTTTG TGGACAACCA GCACGAGTGG TTCCGGTTCA GCGGGCGGGT GCTGGGCTTG 
GCCCTGGTGC ACCAGCACCT GCTCGACGCC TTCTTCACCC GGCCCTTCTA CAAGGCCCTG 
CTCAGGCTGC CCTGCTCGCT GAGCGACCTG GAGTACCTGG ATGCGGAGTT CCACCAGTCG 
CTCGTCTGGC TGAAGGAGAA CGACATCAGC GACATGGGCC TGGAGCTGAG CTTCTCGGTG 
GTGGAGGAGG TGGCCGGCCA CGTGCTAGAG CGGGAGCTCA AGCCGGGCGG GCGCTCCCTG 
CCCGTGTCGG AGCGCAACAA GAAGGAGTAC ATCGAGCGCA TGCTCAAGTG GCGCCTGGAG 
CGGGGCGTTG CCGACCAGAC GGAGTGCCTC GTCAGGGGCT TCTACGAGGT CGTCGACTCT 
CGTCTGGTAT CCGTCTTCGA CGCCCGAGAG CTGGAGCTGG TGATCGCGGG GACGGCGGAG 
ATAGACACGG CGGACTGGAG GAAAAACACT GAGTACCGAT CGGGCTACCA CGACGGCCAC 
CCCGTCATCC AGTGGTTCTG GCTGGCCATT GAGAAATTTG ACAACGAGCG TCGCCTGCGG 
CTGCTCCAGT TTGTCACGGG CACCTCGAGC ATCCCGTACG AGGGCTTCGC CGCGCTGCGC 
GGCAGCAACG GGCCGCGCAA GTTCTGCATC GAGAAGTGGG GGAAGCCCAC CAGCCTGCCC 
AGAGCTCACA CCTGCTTCAA CCGTCTGGAC CTTCCTCCGT ACACGTCCTT CGACATGCTG 
TACGAGAAGC TGCTGCTGGC CGTCGAAGAG TCCAGCACCT TCGGCATCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8033840 ( XM_029993004.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029848864.1 Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OIa16375
Clone ID Related Accession (Same CDS sequence)	XM_029993004.1
Accession Version	XM_029993004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021917992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG GCGCGTGCCG TCGCCGCCGG CCGGCCATGA TTGCCGATCG GGGCCCCCTC 
CACGCGGCCG GCAGTGGGCT CCGACTTTCA GCCGCTACCC CGGCCCCCGT GGCGCCCTCG 
CCACACCAGC AGCAGCAGCC GCCGGTGCCC CGCAGGGGCA CGGGGTCGGT GAGCACGGGT 
TCGTCGCCGC CTCCCCCTGC GCCGGCAGAG GCCCCCGGCG TGCCGCCCCG GCGCCTTCCC 
GCACTACAGG GGCGCTCCAG CCTGAGCGTG TCGCGGTCCC AGTACGTGCT GGGACGCACG 
GAGGCCCCGC TGCTGGTCAG CTGGGACATC CGGGAGGAGG TCTCCAAAGA GGACTGGATT 
GGCCTGTACC GCACGGATGA GACGGATCCT CTGCATTTCC TGGAGCACAA GAACAAGCGA 
TGTTTGAGCA ACACTGTCAA GGGGCAGGTC CTTTGGAGCA TAGACTGCAA GGACGCTTTT 
ACCGAAGAGA GGACCAATGT GTGTTTCCGC TACTACCACG GCCCTACGGG TACCCTGAGG 
GCCGTGAGCC CGGTGGTGAC CGTCTGCAGG TCAGCCGACA CCACTGGAAT CGTGAACGGC 
CACGGGTCCG AGGTCCTGGT CGGTTACTCC TCGCAGCAGA TACCGATGGA GATGGTCAAG 
TTCTATTTGA CCAACCTGCA TGCCCGCAAC CTGAAGAAGG GGATGTTCTT CAGCCCGGAC 
CCGTACGTGA AGATGGGCAT CCAGCCGGGC CTGGGGGACG GGGTCCTGCT GCTGCCCCAC 
CACGGCCAGA TCTGCCGCAC GGGGGCTGCC GAGTCCACCG TCCATCCGTC CTGGCCGGGG 
CAGTTTGAGT TTATCGGATA CCCGTCGGAC GTGCTCGAGT TTGAGGTCAA GGACAAGTTT 
GCCCGGAGCC GCCCCATCAT TGGCCGCTTC CTGGGCAGGC TCGTGGTGCG CATCTGGGAC 
CTGCTGGACC GATGCTCCGG GGGCGTACCG ATGGAGCTCA GCTACACTCT GTCCAACAAG 
ACACCACCCG AGCACATGAC GGGCCAACTC TTCTTCACTT TTGTACTGGA ACATGGGCCA 
GTGGACCCGT GGACCCACAT CCGCCAAGTC CACAATCCGA TCTACGTGTC GGACGTCCCG 
CCGGACCCCT GCCCTTCCAG CCCCCCGTCC TCCGAAGTGC CTCCCCCTCC GCCTTCCACC 
CTGCACCAGC CGCCGCTCAC CGCACGAACG ACCTCCCTCC CCAACGGGAG CGGCCACCAC 
GCCCCTCCGA CCTCCTCCTC CTCTTCCTCG TCCTCCTCCA CCTCCTCGGC AGGAGATCCG 
GAGCCGGCCC CACGGCGGGA CGCCGCACGG AGTCGCAGCG TCCGCTCCAA CAGCGAGCAC 
GGCCTCCACT CACCGGGGGC GGCCCCCCAG GCGGCCTCGC GGCGCCTCTC GCAGACGCCG 
CGACCTCCCG CGAACGCATC GTCGTCGTCC TTCGCGAACC TCGAGTACAT GCTGCAGCGG 
GAGGCCGTCC CCGGGACCGG CGCGGACTTG CCGCCGCCGC TGCCTCCAAA GCAGAAGGGT 
AACCCCCGGC GGCATCACCA TCATCATCAT CACCGGCCGT TGGAGAGGTC GCGCGCGTTG 
GATGTGGCGA GGTCGACGAC GGAGGAGGTG ACTTCTTCGT CGTGGAAGCA CGCGAGACAC 
CGGGAGGCGG GCTCACCGGG ACCGGGCACG GGGGGTGAGG CCGTCGACTC GGACGGGAGT 
TCGGCAAGCT TGGCATCTTC AAGTGCAGCG TCCCCGACAG CGACGACCGG CGAGGAAGAG 
GCGAGCCCGC CCCCAACGCT GCCCCGGAGG ACGGCGAGAC CCACCGTGCT CCAGACAACG 
TCGGGCGAAG CGGGCACCGT CGGCAGCGGG AGCGCAAGGG CGGCCCCCCT GGCGACTCCG 
GAGCAGGACT CGTCGCTCCA GGAGGGGGCA CCCTCCTCCC TGGAGAACCT GAGCGGGGTC 
CTGGACGCCC TGGACTCGGA CGAGGAGGTG GGACGGGCCG AGGACGAACG GTCGGCGTGC 
GACTTTGACC GCGCCAACAG CGAGCCGGCC AACCTGGAGT TCGACGCCGG GGGCGTCGCG 
TCCCCTTCGG CCGGAGACGA GGACAACAGC CCCTCTCCCG CGCCAGGGGG CAGCCGGGGG 
GCCTCGCCGC AGCAGTCATC CCTGGGGGAC TCATCCGTGG ACGCTTCGTC GTCCCAGACG 
CTGAGCGCGA GCGAGGAGGA GGAAGAGGAC AACGCGGAGG CGAGGAACTT CGAGTGTCGA 
GAGCAGTCGT CGCAGCGCAG ACGGCGTCAG ACGCCGCAGC GATGCCACTC GGACTCGGAG 
TGCTCGTTCC CACGCCCGCG CCGGCAGCAC AACCGCCACA GCCACCACCA GGCGCACCAC 
CGGAGGCGGC AGCTAGGAGC CGAGGCCAGG CCAGATCCCC CAAGGCTGGA CCCCTCGTCG 
AGGGCTGCCT TCGTGTTCCC CGTGGAACAG TCCGGCTGCC TGGCGTCGTC CGGCAGCGTG 
CCCCTGCTGC TGCCCTACAG CCAGAGCGAC CACGGCAGCC TGTCCCTGGG TCCCCCCGAG 
GAGGCGTGCC CCTTGGGGCC CCGGGGGGCC CTTTCCCGCA TGCGCATGGG CCCCGAGGAG 
TACGGCAGGC TCCTCCCCGT GGGCGACGGA CTCGTCCTGG CGAGTGTGTC AAGCAACAGA 
ACTTCCACTG TGGAGCCATC CCCCGCTGCG TCGTCGGTTG CCACCCTGAT GAACGTGCAG 
CCGCAGCAGC ACAGCAACAC CACCCGGCTG CCTTCCATAC CGGAGCGTAC CATCCGCTAC 
CAGCGCGTCG AGCTCGAAGA ACCGCTACCC CCCCATTGGG AGGCCCGCAT CGACAGTCAC 
GGACGCGTCT TCTACATTGA CCACCTGAAC CGCACCACCA CGTGGAACCG GCCCTCTCTC 
TCGCAGCCCG TCAGTGTCGT CGCCTGCAAC GAACTCCAGC GGCAACAGTT TGACCGCAGG 
TATCAGAGCA TTCGACGCAC AATGACGCCA CGCATGACGG CCGCGGCTGC GGAACGAGCT 
TCGGCTGCCG CGGCGGCTGC GGCGGCAGCA GCGGCGGCTT CATCTCCGGT GGCGACATGT 
GCCGTGGGCA TGGGCGCGGC CTCCCCTCCG CCTCCTCCGC CCCCAGCAAT CGAGGAGGTT 
GTTCCTGGGA GCTGTTCCTC GGTGGCGCCC TCTGCACCCC CGGCTCCGGC CCCGGCTCCA 
GTGACACCGA GTGCCGACAG CCTAAGGGCG CTGTTGCGGT CGCCCGCAGT TCGCTTTGTT 
ACGCGCTCCG ACTTCTTCAA CGTGCTGCAC ATGAACGACG ATGCCTTGGC TGCTTACAAT 
AGAAGCTCTT CCTTGAAACA CATGATCGCC AAGATCCGGC GAGATTCCGC CAATTTCGAG 
AGGTACCAAC ATAACCGGGA CCTTGTCAGC CTGCTGAACA AATTTGCCGA CAGGGGAAGA 
GAACTGCCTC GTGGCTGGGA GACAAAGCTA GACCGCACGG GAAAGCAATT CTTCATCGAC 
CACACGGCGC GGAGCACGAC GTTCATCGAC CCCCGGCTAC CCATGGACGT GCCCTACTTG 
AACCCGAGCC GACTGGTGGT GCCTTTGGCC AGGAGGCGCA GCCGCAGTGC AGGGGAGGAC 
GACCGGGCAG AGGCCGCTGC CAGGGCCGGA GGAGCGACGG GGGGCCCCGT TCCCCCTCCC 
CGGCCCCCGG CGAGCACGGC GGGGGGCGCG TCTGGGGTGG CGGGCGGGGG CGGCAGCGTG 
CCCACGGCGT ACAACGACAA GGTGGTGGCC TTCCTGCGCC AGCCCAACAT CCTGGACATC 
CTGAGGGAGC GCCACCCGGC CATCGCCTCC AACGCCGCCC TAAGGGACCG CGTCCACTGC 
ATACGCGCCG ACGGGACCGC TGCCCTCGGA AGGCTCTCGC ACGACCTCGA CCTCACCATC 
CTGCTCAGCC TGTTTGAGCA GGAGATCATG TCATACATTC CGGCCCAGTA CACCTCTGGG 
AGCAGTTCTA GTCATCGCTC ACCAAGAGAT TCTCCGCAGC CTTCGCCGCA GGCCTCCCCA 
GGACTTCAAA GGGCCAACGT TCGAGCGCCC GCCCCTTACC GACGGGACTT TGAGGCCAAG 
CTGCGGAACT TCTACCGCAA GCTGGAGTCC AAGGGCTATG GTCAGGGCCC CAGCAAGTTA 
AAACTAAACA TCCGTCGGGA TCACCTACTC GAAGACGCAT TCACAAAGAT AATGGCTGCG 
TCCAAGAAAG ACCTCCAGAA GTCGAGGCTT TACATAGGAT TTGCAGGAGA AGAAGGGCTG 
GACTATGGGG GCCCCTCCCG GGAGTTCTTC TTCCTGCTCT CGCGGGAGCT GTTCAACCCG 
TACTACGGCC TGTTTGAGTA CTCAGCCAAC GACACGTACA CGGTGCAAGT GAGCCCCATG 
GCAGCCTTTG TGGACAACCA GCACGAGTGG TTCCGGTTCA GCGGGCGGGT GCTGGGCTTG 
GCCCTGGTGC ACCAGCACCT GCTCGACGCC TTCTTCACCC GGCCCTTCTA CAAGGCCCTG 
CTCAGGCTGC CCTGCTCGCT GAGCGACCTG GAGTACCTGG ATGCGGAGTT CCACCAGTCG 
CTCGTCTGGC TGAAGGAGAA CGACATCAGC GACATGGGCC TGGAGCTGAG CTTCTCGGTG 
GTGGAGGAGG TGGCCGGCCA CGTGCTAGAG CGGGAGCTCA AGCCGGGCGG GCGCTCCCTG 
CCCGTGTCGG AGCGCAACAA GAAGGAGTAC ATCGAGCGCA TGCTCAAGTG GCGCCTGGAG 
CGGGGCGTTG CCGACCAGAC GGAGTGCCTC GTCAGGGGCT TCTACGAGGT CGTCGACTCT 
CGTCTGGTAT CCGTCTTCGA CGCCCGAGAG CTGGAGCTGG TGATCGCGGG GACGGCGGAG 
ATAGACACGG CGGACTGGAG GAAAAACACT GAGTACCGAT CGGGCTACCA CGACGGCCAC 
CCCGTCATCC AGTGGTTCTG GCTGGCCATT GAGAAATTTG ACAACGAGCG TCGCCTGCGG 
CTGCTCCAGT TTGTCACGGG CACCTCGAGC ATCCCGTACG AGGGCTTCGC CGCGCTGCGC 
GGCAGCAACG GGCCGCGCAA GTTCTGCATC GAGAAGTGGG GGAAGCCCAC CAGCCTGCCC 
AGAGCTCACA CCTGCTTCAA CCGTCTGGAC CTTCCTCCGT ACACGTCCTT CGACATGCTG 
TACGAGAAGC TGCTGCTGGC CGTCGAAGAG TCCAGCACCT TCGGCATCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029848864.1
CDS	137..5410
Translation	MAGGACRRRRPAMIADRGPLHAAGSGLRLSAATPAPVAPSPHQQQQPPVPRRGTGSVSTGSSPPPPAPAEAPGVPPRRLPALQGRSSLSVSRSQYVLGRTEAPLLVSWDIREEVSKEDWIGLYRTDETDPLHFLEHKNKRCLSNTVKGQVLWSIDCKDAFTEERTNVCFRYYHGPTGTLRAVSPVVTVCRSADTTGIVNGHGSEVLVGYSSQQIPMEMVKFYLTNLHARNLKKGMFFSPDPYVKMGIQPGLGDGVLLLPHHGQICRTGAAESTVHPSWPGQFEFIGYPSDVLEFEVKDKFARSRPIIGRFLGRLVVRIWDLLDRCSGGVPMELSYTLSNKTPPEHMTGQLFFTFVLEHGPVDPWTHIRQVHNPIYVSDVPPDPCPSSPPSSEVPPPPPSTLHQPPLTARTTSLPNGSGHHAPPTSSSSSSSSSTSSAGDPEPAPRRDAARSRSVRSNSEHGLHSPGAAPQAASRRLSQTPRPPANASSSSFANLEYMLQREAVPGTGADLPPPLPPKQKGNPRRHHHHHHHRPLERSRALDVARSTTEEVTSSSWKHARHREAGSPGPGTGGEAVDSDGSSASLASSSAASPTATTGEEEASPPPTLPRRTARPTVLQTTSGEAGTVGSGSARAAPLATPEQDSSLQEGAPSSLENLSGVLDALDSDEEVGRAEDERSACDFDRANSEPANLEFDAGGVASPSAGDEDNSPSPAPGGSRGASPQQSSLGDSSVDASSSQTLSASEEEEEDNAEARNFECREQSSQRRRRQTPQRCHSDSECSFPRPRRQHNRHSHHQAHHRRRQLGAEARPDPPRLDPSSRAAFVFPVEQSGCLASSGSVPLLLPYSQSDHGSLSLGPPEEACPLGPRGALSRMRMGPEEYGRLLPVGDGLVLASVSSNRTSTVEPSPAASSVATLMNVQPQQHSNTTRLPSIPERTIRYQRVELEEPLPPHWEARIDSHGRVFYIDHLNRTTTWNRPSLSQPVSVVACNELQRQQFDRRYQSIRRTMTPRMTAAAAERASAAAAAAAAAAAASSPVATCAVGMGAASPPPPPPPAIEEVVPGSCSSVAPSAPPAPAPAPVTPSADSLRALLRSPAVRFVTRSDFFNVLHMNDDALAAYNRSSSLKHMIAKIRRDSANFERYQHNRDLVSLLNKFADRGRELPRGWETKLDRTGKQFFIDHTARSTTFIDPRLPMDVPYLNPSRLVVPLARRRSRSAGEDDRAEAAARAGGATGGPVPPPRPPASTAGGASGVAGGGGSVPTAYNDKVVAFLRQPNILDILRERHPAIASNAALRDRVHCIRADGTAALGRLSHDLDLTILLSLFEQEIMSYIPAQYTSGSSSSHRSPRDSPQPSPQASPGLQRANVRAPAPYRRDFEAKLRNFYRKLESKGYGQGPSKLKLNIRRDHLLEDAFTKIMAASKKDLQKSRLYIGFAGEEGLDYGGPSREFFFLLSRELFNPYYGLFEYSANDTYTVQVSPMAAFVDNQHEWFRFSGRVLGLALVHQHLLDAFFTRPFYKALLRLPCSLSDLEYLDAEFHQSLVWLKENDISDMGLELSFSVVEEVAGHVLERELKPGGRSLPVSERNKKEYIERMLKWRLERGVADQTECLVRGFYEVVDSRLVSVFDARELELVIAGTAEIDTADWRKNTEYRSGYHDGHPVIQWFWLAIEKFDNERRLRLLQFVTGTSSIPYEGFAALRGSNGPRKFCIEKWGKPTSLPRAHTCFNRLDLPPYTSFDMLYEKLLLAVEESSTFGIE

Target ORF information:

RefSeq Version	XM_029993004.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis E3 ubiquitin-protein ligase HECW2 (LOC8033840), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029993004.1

ORF Insert Sequence:

ATGGCAGGGG GCGCGTGCCG TCGCCGCCGG CCGGCCATGA TTGCCGATCG GGGCCCCCTC 
CACGCGGCCG GCAGTGGGCT CCGACTTTCA GCCGCTACCC CGGCCCCCGT GGCGCCCTCG 
CCACACCAGC AGCAGCAGCC GCCGGTGCCC CGCAGGGGCA CGGGGTCGGT GAGCACGGGT 
TCGTCGCCGC CTCCCCCTGC GCCGGCAGAG GCCCCCGGCG TGCCGCCCCG GCGCCTTCCC 
GCACTACAGG GGCGCTCCAG CCTGAGCGTG TCGCGGTCCC AGTACGTGCT GGGACGCACG 
GAGGCCCCGC TGCTGGTCAG CTGGGACATC CGGGAGGAGG TCTCCAAAGA GGACTGGATT 
GGCCTGTACC GCACGGATGA GACGGATCCT CTGCATTTCC TGGAGCACAA GAACAAGCGA 
TGTTTGAGCA ACACTGTCAA GGGGCAGGTC CTTTGGAGCA TAGACTGCAA GGACGCTTTT 
ACCGAAGAGA GGACCAATGT GTGTTTCCGC TACTACCACG GCCCTACGGG TACCCTGAGG 
GCCGTGAGCC CGGTGGTGAC CGTCTGCAGG TCAGCCGACA CCACTGGAAT CGTGAACGGC 
CACGGGTCCG AGGTCCTGGT CGGTTACTCC TCGCAGCAGA TACCGATGGA GATGGTCAAG 
TTCTATTTGA CCAACCTGCA TGCCCGCAAC CTGAAGAAGG GGATGTTCTT CAGCCCGGAC 
CCGTACGTGA AGATGGGCAT CCAGCCGGGC CTGGGGGACG GGGTCCTGCT GCTGCCCCAC 
CACGGCCAGA TCTGCCGCAC GGGGGCTGCC GAGTCCACCG TCCATCCGTC CTGGCCGGGG 
CAGTTTGAGT TTATCGGATA CCCGTCGGAC GTGCTCGAGT TTGAGGTCAA GGACAAGTTT 
GCCCGGAGCC GCCCCATCAT TGGCCGCTTC CTGGGCAGGC TCGTGGTGCG CATCTGGGAC 
CTGCTGGACC GATGCTCCGG GGGCGTACCG ATGGAGCTCA GCTACACTCT GTCCAACAAG 
ACACCACCCG AGCACATGAC GGGCCAACTC TTCTTCACTT TTGTACTGGA ACATGGGCCA 
GTGGACCCGT GGACCCACAT CCGCCAAGTC CACAATCCGA TCTACGTGTC GGACGTCCCG 
CCGGACCCCT GCCCTTCCAG CCCCCCGTCC TCCGAAGTGC CTCCCCCTCC GCCTTCCACC 
CTGCACCAGC CGCCGCTCAC CGCACGAACG ACCTCCCTCC CCAACGGGAG CGGCCACCAC 
GCCCCTCCGA CCTCCTCCTC CTCTTCCTCG TCCTCCTCCA CCTCCTCGGC AGGAGATCCG 
GAGCCGGCCC CACGGCGGGA CGCCGCACGG AGTCGCAGCG TCCGCTCCAA CAGCGAGCAC 
GGCCTCCACT CACCGGGGGC GGCCCCCCAG GCGGCCTCGC GGCGCCTCTC GCAGACGCCG 
CGACCTCCCG CGAACGCATC GTCGTCGTCC TTCGCGAACC TCGAGTACAT GCTGCAGCGG 
GAGGCCGTCC CCGGGACCGG CGCGGACTTG CCGCCGCCGC TGCCTCCAAA GCAGAAGGGT 
AACCCCCGGC GGCATCACCA TCATCATCAT CACCGGCCGT TGGAGAGGTC GCGCGCGTTG 
GATGTGGCGA GGTCGACGAC GGAGGAGGTG ACTTCTTCGT CGTGGAAGCA CGCGAGACAC 
CGGGAGGCGG GCTCACCGGG ACCGGGCACG GGGGGTGAGG CCGTCGACTC GGACGGGAGT 
TCGGCAAGCT TGGCATCTTC AAGTGCAGCG TCCCCGACAG CGACGACCGG CGAGGAAGAG 
GCGAGCCCGC CCCCAACGCT GCCCCGGAGG ACGGCGAGAC CCACCGTGCT CCAGACAACG 
TCGGGCGAAG CGGGCACCGT CGGCAGCGGG AGCGCAAGGG CGGCCCCCCT GGCGACTCCG 
GAGCAGGACT CGTCGCTCCA GGAGGGGGCA CCCTCCTCCC TGGAGAACCT GAGCGGGGTC 
CTGGACGCCC TGGACTCGGA CGAGGAGGTG GGACGGGCCG AGGACGAACG GTCGGCGTGC 
GACTTTGACC GCGCCAACAG CGAGCCGGCC AACCTGGAGT TCGACGCCGG GGGCGTCGCG 
TCCCCTTCGG CCGGAGACGA GGACAACAGC CCCTCTCCCG CGCCAGGGGG CAGCCGGGGG 
GCCTCGCCGC AGCAGTCATC CCTGGGGGAC TCATCCGTGG ACGCTTCGTC GTCCCAGACG 
CTGAGCGCGA GCGAGGAGGA GGAAGAGGAC AACGCGGAGG CGAGGAACTT CGAGTGTCGA 
GAGCAGTCGT CGCAGCGCAG ACGGCGTCAG ACGCCGCAGC GATGCCACTC GGACTCGGAG 
TGCTCGTTCC CACGCCCGCG CCGGCAGCAC AACCGCCACA GCCACCACCA GGCGCACCAC 
CGGAGGCGGC AGCTAGGAGC CGAGGCCAGG CCAGATCCCC CAAGGCTGGA CCCCTCGTCG 
AGGGCTGCCT TCGTGTTCCC CGTGGAACAG TCCGGCTGCC TGGCGTCGTC CGGCAGCGTG 
CCCCTGCTGC TGCCCTACAG CCAGAGCGAC CACGGCAGCC TGTCCCTGGG TCCCCCCGAG 
GAGGCGTGCC CCTTGGGGCC CCGGGGGGCC CTTTCCCGCA TGCGCATGGG CCCCGAGGAG 
TACGGCAGGC TCCTCCCCGT GGGCGACGGA CTCGTCCTGG CGAGTGTGTC AAGCAACAGA 
ACTTCCACTG TGGAGCCATC CCCCGCTGCG TCGTCGGTTG CCACCCTGAT GAACGTGCAG 
CCGCAGCAGC ACAGCAACAC CACCCGGCTG CCTTCCATAC CGGAGCGTAC CATCCGCTAC 
CAGCGCGTCG AGCTCGAAGA ACCGCTACCC CCCCATTGGG AGGCCCGCAT CGACAGTCAC 
GGACGCGTCT TCTACATTGA CCACCTGAAC CGCACCACCA CGTGGAACCG GCCCTCTCTC 
TCGCAGCCCG TCAGTGTCGT CGCCTGCAAC GAACTCCAGC GGCAACAGTT TGACCGCAGG 
TATCAGAGCA TTCGACGCAC AATGACGCCA CGCATGACGG CCGCGGCTGC GGAACGAGCT 
TCGGCTGCCG CGGCGGCTGC GGCGGCAGCA GCGGCGGCTT CATCTCCGGT GGCGACATGT 
GCCGTGGGCA TGGGCGCGGC CTCCCCTCCG CCTCCTCCGC CCCCAGCAAT CGAGGAGGTT 
GTTCCTGGGA GCTGTTCCTC GGTGGCGCCC TCTGCACCCC CGGCTCCGGC CCCGGCTCCA 
GTGACACCGA GTGCCGACAG CCTAAGGGCG CTGTTGCGGT CGCCCGCAGT TCGCTTTGTT 
ACGCGCTCCG ACTTCTTCAA CGTGCTGCAC ATGAACGACG ATGCCTTGGC TGCTTACAAT 
AGAAGCTCTT CCTTGAAACA CATGATCGCC AAGATCCGGC GAGATTCCGC CAATTTCGAG 
AGGTACCAAC ATAACCGGGA CCTTGTCAGC CTGCTGAACA AATTTGCCGA CAGGGGAAGA 
GAACTGCCTC GTGGCTGGGA GACAAAGCTA GACCGCACGG GAAAGCAATT CTTCATCGAC 
CACACGGCGC GGAGCACGAC GTTCATCGAC CCCCGGCTAC CCATGGACGT GCCCTACTTG 
AACCCGAGCC GACTGGTGGT GCCTTTGGCC AGGAGGCGCA GCCGCAGTGC AGGGGAGGAC 
GACCGGGCAG AGGCCGCTGC CAGGGCCGGA GGAGCGACGG GGGGCCCCGT TCCCCCTCCC 
CGGCCCCCGG CGAGCACGGC GGGGGGCGCG TCTGGGGTGG CGGGCGGGGG CGGCAGCGTG 
CCCACGGCGT ACAACGACAA GGTGGTGGCC TTCCTGCGCC AGCCCAACAT CCTGGACATC 
CTGAGGGAGC GCCACCCGGC CATCGCCTCC AACGCCGCCC TAAGGGACCG CGTCCACTGC 
ATACGCGCCG ACGGGACCGC TGCCCTCGGA AGGCTCTCGC ACGACCTCGA CCTCACCATC 
CTGCTCAGCC TGTTTGAGCA GGAGATCATG TCATACATTC CGGCCCAGTA CACCTCTGGG 
AGCAGTTCTA GTCATCGCTC ACCAAGAGAT TCTCCGCAGC CTTCGCCGCA GGCCTCCCCA 
GGACTTCAAA GGGCCAACGT TCGAGCGCCC GCCCCTTACC GACGGGACTT TGAGGCCAAG 
CTGCGGAACT TCTACCGCAA GCTGGAGTCC AAGGGCTATG GTCAGGGCCC CAGCAAGTTA 
AAACTAAACA TCCGTCGGGA TCACCTACTC GAAGACGCAT TCACAAAGAT AATGGCTGCG 
TCCAAGAAAG ACCTCCAGAA GTCGAGGCTT TACATAGGAT TTGCAGGAGA AGAAGGGCTG 
GACTATGGGG GCCCCTCCCG GGAGTTCTTC TTCCTGCTCT CGCGGGAGCT GTTCAACCCG 
TACTACGGCC TGTTTGAGTA CTCAGCCAAC GACACGTACA CGGTGCAAGT GAGCCCCATG 
GCAGCCTTTG TGGACAACCA GCACGAGTGG TTCCGGTTCA GCGGGCGGGT GCTGGGCTTG 
GCCCTGGTGC ACCAGCACCT GCTCGACGCC TTCTTCACCC GGCCCTTCTA CAAGGCCCTG 
CTCAGGCTGC CCTGCTCGCT GAGCGACCTG GAGTACCTGG ATGCGGAGTT CCACCAGTCG 
CTCGTCTGGC TGAAGGAGAA CGACATCAGC GACATGGGCC TGGAGCTGAG CTTCTCGGTG 
GTGGAGGAGG TGGCCGGCCA CGTGCTAGAG CGGGAGCTCA AGCCGGGCGG GCGCTCCCTG 
CCCGTGTCGG AGCGCAACAA GAAGGAGTAC ATCGAGCGCA TGCTCAAGTG GCGCCTGGAG 
CGGGGCGTTG CCGACCAGAC GGAGTGCCTC GTCAGGGGCT TCTACGAGGT CGTCGACTCT 
CGTCTGGTAT CCGTCTTCGA CGCCCGAGAG CTGGAGCTGG TGATCGCGGG GACGGCGGAG 
ATAGACACGG CGGACTGGAG GAAAAACACT GAGTACCGAT CGGGCTACCA CGACGGCCAC 
CCCGTCATCC AGTGGTTCTG GCTGGCCATT GAGAAATTTG ACAACGAGCG TCGCCTGCGG 
CTGCTCCAGT TTGTCACGGG CACCTCGAGC ATCCCGTACG AGGGCTTCGC CGCGCTGCGC 
GGCAGCAACG GGCCGCGCAA GTTCTGCATC GAGAAGTGGG GGAAGCCCAC CAGCCTGCCC 
AGAGCTCACA CCTGCTTCAA CCGTCTGGAC CTTCCTCCGT ACACGTCCTT CGACATGCTG 
TACGAGAAGC TGCTGCTGGC CGTCGAAGAG TCCAGCACCT TCGGCATCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

FAX

1-732-210-0262

LOC8033840 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

FAX